id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260071-z29b30sd	Zhong, Yu	Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton	2005-03-27		.txt	text/plain	5477	274	60	All of these compounds are assumed to exert their anti-HIV activity by shielding the positively charged sites in the V3 loop region of the viral gp120 envelope glycoprotein, and interrupting virus attachment to the negatively charged heparan sulfate proteoglycans on cell surface, and inhibiting the specific binding to the CD4 receptor of CD4 + cells. PHA (1 g/ml, Sigma-Aldrich)/IL-2 (100 U/ml, Shionogi, Osaka, Japan) activated PBMCs were infected for 2 h with 20 ng of HIV-1 p24 Gag (X4/NL4-3 or R5/JRCSF) in the presence or absence of the Sukumo extract (0.64-400 g/ml), washed three times with PBS and cultured for 7 days in RPMI-1640 medium/10% FBS plus 100 U/ml IL-2 with or without the Sukumo extract. Sukumo extract was also evaluated for the inhibition of wild-type herpes simplex virus-1 replication in infected Vero cells, using a standard viral plaque assay (Fig. 1B) .	./cache/cord-260071-z29b30sd.txt	./txt/cord-260071-z29b30sd.txt