id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-256280-ihj102hi	Takano, Tomomi	The cholesterol transport inhibitor U18666A inhibits type I feline coronavirus infection	2017-08-03		.txt	text/plain	4180	264	54	title: The cholesterol transport inhibitor U18666A inhibits type I feline coronavirus infection In this study, we investigated whether U18666A, the cholesterol synthesis and transport inhibitor, shows antiviral effects against type I FCoV. U18666A induced cholesterol accumulation in cells and inhibited type I FCoV replication. These findings suggest that cell membrane cholesterol plays an important role in type I FCoV infection. In order to confirm the effects of the accumulation of cholesterol on FCoV infection, fcwf-4 cells were pretreated with U18666A, followed by virus inoculation. When cells were pretreated with cholesterol biosynthesis inhibitors, only itraconazole significantly inhibited FCoV-I KU2 plaque formation. In addition, treatment of cells with U18666A did not influence the cellular cholesterol level, suggesting that U18666A inhibits type I FCoV replication at a concentration suppressing cholesterol transporter but not influencing the synthesis system. It was suggested that U18666A inhibits type I FCoV replication by suppressing the intracellular cholesterol transporter.	./cache/cord-256280-ihj102hi.txt	./txt/cord-256280-ihj102hi.txt