id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-010247-cug21fnf	Hollingshead, Melinda G.	An ELISA system for evaluating antiretroviral activity against Rauscher murine leukemia virus	1992-06-15		.txt	text/plain	2412	141	54	A system for evaluating the activity of antiviral agents against Rauscher murine leukemia virus (R-MuLV) has been developed using an enzyme linked immunosorbent assay technique. The assay is based upon detection of R-MuLV encoded p30 protein production in virus infected murine cells. Cytotoxicity evaluations are conducted in parallel to the Rauscher MuLV ELISA assay in order to assess drug-induced reductions in cell viability. Cytotoxicity evaluations are important to interpretation of the ELISA results since reductions in cell viability reduce viral protein production which would indicate an antiviral drug effect. In the case of this compound, the activity detected by the UV-XC plaque reduction assay was believed to result from cytotoxicity to the XC cells used as an overlay. The primary difference between the-ELISA assay and the UV-XC plaque reduction assay is in the drug concentrations required to suppress the virus production endpoint. The UV-XC plaque reduction assay measures the capacity of an antiviral compound to reduce production of infectious virus.	./cache/cord-010247-cug21fnf.txt	./txt/cord-010247-cug21fnf.txt