id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-012826-72mz834w	Xu, Zhen-dong	Propofol affects mouse embryonic fibroblast survival and proliferation in vitro via ATG5- and calcium-dependent regulation of autophagy	2019-10-23		.txt	text/plain	4776	256	49	In addition, ATG5(−/−) MEF themselves released more Ca(2+) in cytosolic space and endoplasmic reticulum compared with WT cells, suggesting that autophagy deficiency made intracellular calcium signaling more vulnerable to external stimuli (propofol). After 24-h exposure to propofol, the MTT signal of ATG5 −/− cells was significantly enhanced at clinically relevant concentrations (10 µM); however, this effect was inverted at high pharmacological General anesthetics and autophagy ZD Xu et al. ATG5 plays a key role in propofol effects on cell growth We next determined whether the elevation of MTT by propofol at clinically relevant concentrations in ATG5 −/− cells (Fig. 1c) was caused by changes in mitochondrial reductase activity (early cell damage) or an increase in cell numbers (proliferation). While this enhances vulnerability to cell death triggered by calcium overload at high propofol doses, propofol at clinically relevant concentrations (10 μM) induces a moderate increase in Ca 2+ release from the ER into the cytosol, favoring cell survival and proliferation of autophagy-deficient cells (Fig. 6) .	./cache/cord-012826-72mz834w.txt	./txt/cord-012826-72mz834w.txt