id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-012768-9fvumvdc	Chen, Bao-yi	SGK1 mediates the hypotonic protective effect against H(2)O(2)-induced apoptosis of rat basilar artery smooth muscle cells by inhibiting the FOXO3a/Bim signaling pathway	2020-03-05		.txt	text/plain	6088	331	47	title: SGK1 mediates the hypotonic protective effect against H(2)O(2)-induced apoptosis of rat basilar artery smooth muscle cells by inhibiting the FOXO3a/Bim signaling pathway Our previous study shows that activation of volume-regulated Cl(−) channels (VRCCs) protects rat basilar artery smooth muscle cells (BASMCs) against hydrogen peroxide (H(2)O(2))-induced apoptosis. The protective effect of hypotonic challenge against H(2)O(2)-induced apoptosis was mediated through inhibiting mitochondria-dependent apoptotic pathway, evidenced by increased Bcl-2/Bax ratio, stabilizing mitochondrial membrane potential (MMP), decreased cytochrome c release from the mitochondria to the cytoplasm, and inhibition of the activation of caspase-9 and caspase-3. Thus, we further explored whether the underlying mechanism of the protective effect of SGK1 on H 2 O 2induced apoptosis is related to the inhibition of the FOXO3a/Bim signaling pathway in BASMCs. Reagents and antibodies Cell culture medium (Dulbecco's modified Eagle's medium: nutrient mixture F-12 (DMEM/F-12)), fetal calf serum, bovine serum albumin (BSA), and protease inhibitor cocktail were obtained from GIBCO/Invitrogen (Carlsbad, CA, USA).	./cache/cord-012768-9fvumvdc.txt	./txt/cord-012768-9fvumvdc.txt