id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002006-pwlybr2h	Liu, Yuan-yuan	Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo	2016-03-14		.txt	text/plain	3906	197	50	AIM: To investigate the antiviral effects of vectors expressing specific short hairpin RNAs (shRNAs) against Hantaan virus (HTNV) infection in vitro and in vivo. In mice infected with lethal doses of HTNV, intraperitoneal injection of pSilencer-S or pSilencer-M (30 μg) considerably increased the survival rates and mean time to death, and significantly reduced the mean virus yields and viral RNA level, and alleviated virus-induced pathological lesions in lungs, brains and kidneys. Based on these results and the viral antigen expression results detected by IFA (data not shown), we concluded that the shRNAs that targeted the S and M segments of the HTNV gene were able to inhibit RNA transcript and virus production in the HTNV-infected cells and that shRNA-S1 and shRNA-M2 exhibited a stronger inhibitory effect against HTNV. The RNAi pSilencer-S and pSilencer-M plasmids were constructed, and their antiviral effects were further evaluated by detecting the viral protein synthesis and RNA transcript and progeny virus titers in the HTNV-infected cells.	./cache/cord-002006-pwlybr2h.txt	./txt/cord-002006-pwlybr2h.txt