Acta Derm Venereol 94 SHORT COMMUNICATION Acta Derm Venereol 2014; 94: 585–587 © 2014 The Authors. doi: 10.2340/00015555-1740 Journal Compilation © 2014 Acta Dermato-Venereologica. ISSN 0001-5555 Despite their limitations and side-effects whole-body UVB phototherapy and corticosteroids are still the mainstay in first-line treatment of vitiligo in both adults and children (1). As vitiligo usually does not affect more than 20% of the body surface, topical treatment strate- gies have become the focus of interest. Several clinical studies have recently shown important advantages of focused broad- (BB) or narrow-band (NB) UVB as they allow selective treatment of target lesions, along with an energy adaptation to the respective body part while sparing the normally pigmented surrounding skin (2–4). Given twice weekly up to every second week for less than 60 sessions they allow both the reduction of number of treatments as well as the cumulative doses along with higher efficiency. Furthermore, calcineurin antagonists like tacrolimus and pimecrolimus were introduced as topical immunomodulators for vitiligo and proved to be equally effective as clobetasol (5–8). Using overnight occlusive hydrocolloid dressings they proved to be ef- fective and safe also in poorly repigmenting body areas like forearms and shins (4, 5). Recently, it was shown that tacrolimus increases pigmentation and migration of human melanocytes (9). Until now, it is not known whether either of the 2 treatment options is generally superior to the other or whether intra-individual responses will be similar or different. In a small case study we compared for the first time the efficacy of targeted intense pulsed light (IPL) phototherapy versus tacrolimus 0.1% ointment in an intra-individual right–left comparative way for up to 9 months. MATERIALS AND METHODS Eleven patients (8 women, 3 men; 15–69 years old; mean 41 ± 19), with symmetrical vitiligo of < 20% body surface area (BSA), were treated after they or their parents had signed detailed written informed consent. They had Fitzpatrick skin phototype I–IV and disease duration of 8 months up to 30 years (mean 11.1 ± 10 years). Patients with a tendency to spontaneous repigmentation, those who had received topical or systemic treatment during the last 6 months, pregnant women, and pa- tients with history of premalignant or malignant skin lesions were excluded. Tacrolimus 0.1% ointment (Protopic®, Astellas Pharma GmbH, Munich, Germany) was applied twice daily on the depigmented lesions on the face, trunk, and extremities of the right side, combined with overnight hydrocolloid dres- sing (Comfeel plus transparent, Coloplast GmbH, Hamburg, Germany) on the shins. Targeted phototherapy was performed once weekly on the respective lesions of the left side, using a BBUVB light source with peak emission at 311 nm (Relume- Mode, Lumenis). Treatment of hands and feet was spared, as these regions are known to be treatment resistant (3, 5–7). If repigmentation was not visible after 6 months the treatment was stopped. Otherwise, after 9 months therapy was continued with the most effective regimen on both treatment sides. Patients were free to withdraw at any time during the treat- ment procedure. All procedures were in accordance with the ethical standards on human experimentation and with the Helsinki Declaration, as revised in 2008. Baseline photographs performed by digital photography using natural as well as Wood’s light were performed at the beginning of the treatment and were compared with photographs after 3, 6, and 9 months, and at the end of treatment, respectively. Precent repigmenta- tion was evaluated. Because affected BSA was < 10% in all patients and as we performed a right–left comparative treat- ment we calculated percent repigmentation for each treated body region instead of percentage of total vitiligo body area, as recommended by the Vitiligo European Task Force (VETF). For grading the following generally accepted scale was used: minimal < 25%; moderate: 25–50%; good: 50–75%; excellent: > 75%. In addition, repigmentation was also assessed by the 0–3 VETF staging score as recommended (10). To the best of our knowledge, this score has been used so far only in one pilot clinical trial but did not show significant improvement (11). For statistical evaluation, the student t-test was used to calculate the difference between the means of 2 independent samples. The significance level was set at p = 0.05. RESULTS In the 11 patients vitiligo lesions in 35 different body areas (1–325 cm2) were treated. Mean starting fluence was 50.5 ± 17.6 mJ/cm2 in UV-sensitive regions (face, neck, trunk, upper arms, and thighs) and 87.7 ± 34.4 mJ/ cm² in UV-insensitive areas (forearms, elbows, knees, lower extremities), respectively. After 9 months, mean highest fluence was 277 ± 202 mJ/cm2 in UV-sensitive and 705 ± 535 mJ/cm2 in insensitive areas, respectively. After 6 months, 2 of 11 patients did not show any repigmentation and thus treatment was stopped. In the remaining 9 patients, mean time of initial repigmenta- tion on the tacrolimus-treated side was comparable to the UVB-treated side, even if face, trunk and extremities were considered separately (face: tacrolimus 8.0 ± 1.0 weeks versus UVB-IPL 9.3 ± 4.9 weeks, p = 0.69; trunk: tacrolimus 10.0 ± 2.0 weeks versus UVB-IPL 10.0 ± 5.4 weeks, p = 0.73; extremities: tacrolimus 14.8 ± 5.8 weeks versus UVB-IPL 14.3 ± 6.2 weeks, p = 0.99). After 6 months, the mean percentage of repigmentation of all Treatment of Generalised Vitiligo with Tacrolimus 0.1% Ointment vs. UVB Intense Pulsed Light Phototherapy: A Pilot Study Anke Hartmann, Lisa Löhberg, Petra Keikavoussi, Saskia Eichner and Gerold Schuler Department of Dermatology, University Hospital Erlangen, Ulmenweg 18, DE-91054 Erlangen, Germany. E-mail: anke.hartmann@uk-erlangen.de Accepted Aug 18, 2013; Epub ahead of print Jan 28, 2014 mailto:anke.hartmann@uk-erlangen.de 586 Short communication treated body sites, non-responding lesions included, was comparable between tacrolimus- and UVB-treated lesions (Fig. 1). After 9 months, 38% of lesions on the tacrolimus-treated side on the trunk showed repig- mentation compared to 63% on the UVB-treated side, whereas on face and extremities 67% and 80% of the lesions on both treatment sides responded. Mean per- centage of repigmentation on the face and extremities was higher on the tacrolimus-treated side compared to the UVB-treated side; however, the differences were not statistically significant (Fig. 1). According to the VETF staging scale mean staging sco- re before and after treatment was similar for each side of the body (1.85 ± 0.4 on the tacrolimus side and 1.85 ± 0.5 on the UVB-treated side at the start, and 1.25 ± 0.6 and 1.25 ± 0.7 at the end of treatment, respectively). Thus, mean decrease was 0.6, translating to a significant 32% improvement (p = 0.001 and 0.003, respectively). Four patients showed moderate to excellent repig- mentation after treatment with either tacrolimus or UVB-IPL, respectively; however, on the respective opposite region there was only minimal or no reaction (Fig. S1A1 and Fig. S21). Five patients showed repig- mentation with both treatment modalities; however, the bilateral responses were intra-individually considerably different (Fig. S1B1, Fig. S31), with either a follicular or a homogeneous interfollicular pattern of repigmentation or a mixture of both, without any correlation to treat- ment mode or localisation of the respective area. After 9 months, 4 patients continued with the more effective regimen on both treatment sides for up to additional 9 months and, interestingly, all revealed repigmentation similar to the original opposite side (Fig. S2C1). Side effects included initial mild prickling on the tacrolimus- treated and transient erythema and perilesional tanning on the left side. DISCUSSION In our pilot study we compared for the first time 2 in- creasingly accepted localised approaches for vitiligo treatment in patients with generalised vitiligo in a direct right/left fashion. Long-term treatment with weekly sessions of targeted UVB-IPL phototherapy, as well as twice daily tacrolimus 0.1% ointment proved overall to be comparably effective as measured by mean time to initial repigmentation and mean percent repigmen- tation. Our pilot study in 11 patients, although small, clearly indicated that intra-individual differences occur and that treatment response to one of the topical ap- proaches does not generally imply response to another treatment as well. Although it is known that tacrolimus – in contrast to corticosteroids – does not penetrate through an intact skin barrier and into the circulation, the possibility and relevance of systemic effects of either of the 2 topical treatment approaches cannot be completely excluded (6, 12). Importantly, for the in- dividual patient, response to either of the 2 treatments is currently not predictable and may vary considerably between different body areas. Given these facts it is not surprising that the results of inter-individual com- parative studies were often incongruent, and would require very large cohorts to come to reliable results. Our findings confirm the importance of intra-individual right/left comparative studies, which has also been sug- gested by others (13). Our preliminary results should be confirmed in a larger prospective, randomised clinical trial, which may also allow to outline urgently needed parameters predicting responses. REFERENCES 1. Gawkrodger DJ, Ormerod AD, Shaw L, Mauri-Sole I, Whit- ton ME, Watts MJ, et al. Vitiligo: concise evidence based guidelines on diagnosis and management. Postgrad Med J 2010; 86: 466–471. 2. Hofer A, Hassan AS, Legat FJ, Kerl H, Wolf P. The efficacy of excimer laser (308 nm) for vitiligo at different body sites. J Eur Acad Dermatol Venereol 2006; 20: 558–564. 3. Welsh O, Herz-Ruelas ME, Gómez M, Ocampo-Candiani J. 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Mean percentage repigmentation of lesions on the face, trunk and extremities after 6 months (non-responders included) and 9 months treatment (without non-responder, which stopped treatment after 6 months) with tacrolimus 0.1% ointment (right side of the body) versus targeted UVB-phototherapy (left side of the body), respectively. 1http://www.medicaljournals.se/acta/content/?doi=10.2340/00015555-1740 Acta Derm Venereol 94 http://www.medicaljournals.se/acta/content/?doi=10.2340/00015555-1740 http://www.medicaljournals.se/acta/content/?doi=10.2340/00015555-1740 http://www.medicaljournals.se/acta/content/?doi=10.2340/00015555-1740 http://www.medicaljournals.se/acta/content/?doi=10.2340/00015555-1740 http://www.medicaljournals.se/acta/content/?doi=10.2340/00015555-1740 587Short communication domized trial of 0.1% tacrolimus vs 0.05% clobetasol for the treatment of childhood vitiligo. Arch Dermatol 2003; 139: 581–585. 6. Hartmann A, Bröcker EB, Hamm H. 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