A study of distribution, sex differences and stability of lip print patterns in an Indian population Saudi Journal of Biological Sciences (2015) xxx, xxx–xxx King Saud University Saudi Journal of Biological Sciences www.ksu.edu.sa www.sciencedirect.com ORIGINAL ARTICLE A study of distribution, sex differences and stability of lip print patterns in an Indian population * Corresponding author at: Department of Forensic Science, Govt. Institute of Forensic Science, R.T. Road, Civil Lines, Nagpur, Maharashtra 440001, India. Tel.: +91 7387490889. E-mail addresses: Neeti.kapoor86@gmail.com (N. Kapoor), Badiye. ashish@gmail.com (A. Badiye). Peer review under responsibility of King Saud University. Production and hosting by Elsevier http://dx.doi.org/10.1016/j.sjbs.2015.01.014 1319-562X ª 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Please cite this article in press as: Kapoor, N., Badiye, A. A study of distribution, sex differences and stability of lip print patterns in an Indian populatio Journal of Biological Sciences (2015), http://dx.doi.org/10.1016/j.sjbs.2015.01.014 Neeti Kapoor, Ashish Badiye * Department of Forensic Science, Govt. Institute of Forensic Science, Nagpur, Maharashtra, India Received 12 November 2014; revised 1 January 2015; accepted 24 January 2015 KEYWORDS Cheiloscopy; Lip print pattern; Forensic identification; Sex differences; Marathi population Abstract Lip prints are very useful in forensic investigations. The objective of this study is to deter- mine predominant lip print pattern found among a central Indian population, to evaluate whether any sex difference exists and to study the permanence of the pattern over a 6 month duration. This study included 200 healthy adult subjects comprising of 100 males and 100 females in the age group of 18–25 years. A convenient and easier method of data collection i.e., digital photography was used instead of the traditional lipstick methods. Lip prints were then divided into four quadrants and recognized as per Suzuki and Tsuchihashi’s classification. Type I (30.63%) was found to be most predominant overall in the Marathi population. Type I (29.75%) and Type III (35.75%) were found most prevalent in males and females respectively. Applying the Chi-Square test, statistically significant differences (p < 0.05) were observed between male and female lip print patterns in each of the quadrants individually and all quadrants taken together. The lip print patterns remained stable over a period of six-months. Being stable and with significant sex differences, lip prints can be effectively used as an important tool in forensic investigations for individualization as well as identification of sex of the donor, thus, narrowing down the scope of investigation to almost half. ª 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 1. Introduction In various civil, criminal and mass disaster cases, positive identification of a person can be very difficult. Out of the many existing techniques implied for the purpose, comparison of fingerprints, DNA and dental records are probably the most common techniques used in this context. However, ‘human lip recognition’ also known as cheiloscopy, is one of the most interesting emerging fields which find its roots in criminal and forensic practices (Caldas et al., 2007; Sharma et al., 2009; Reddy and Reddy, 2011). Previous studies have shown to establish the fact that lip prints can positively distinguish indi- viduals and hence have potential use in human identification n. Saudi http://creativecommons.org/licenses/by-nc-nd/4.0/ mailto:Neeti.kapoor86@gmail.com mailto:Badiye.ashish@gmail.com mailto:Badiye.ashish@gmail.com http://dx.doi.org/10.1016/j.sjbs.2015.01.014 http://dx.doi.org/10.1016/j.sjbs.2015.01.014 http://www.sciencedirect.com/science/journal/1319562X http://dx.doi.org/10.1016/j.sjbs.2015.01.014 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://dx.doi.org/10.1016/j.sjbs.2015.01.014 2 N. Kapoor, A. Badiye (Venkatesh and David, 2011; Prabhu et al., 2012, 2013; Dwivedi et al., 2013). The term ‘‘Cheiloscopy’’ is derived from the Greek words cheilos meaning ‘lips’ and e skopein meaning ‘to see’ and is defined as the study of the characteristic patterns of the wrin- kles and grooves present on the labial mucosa (sulci laborium), called as lip prints (Sivapathasundharam et al., 2001; Molano et al., 2002; Rajendran and Sivapathasundharam, 2006; Shafer et al., 2009). R. Fischer was among the first to take notice of the biological phenomenon of systems of furrows on the red part of human lips in the year 1902 (Thomas and Van Wyk, 1988; Kasprzak, 1990, 2000). Use of lip prints in personal iden- tification and criminalization was first recommended in France by Edmond Locard as early as 1932 (Warren, 1976; Thomas and Van Wyk, 1988). Le Moyne Snyder was the first to intro- duce a case in which lip prints helped the crime investigators in an unusual way (Suzuki and Tsuchihashi, 1970a,b; Williams, 1991; Ball, 2002). Santos, Suzuki and Tsuchihashi were among the first to classify the various patterns present on the human lips (Suzuki and Tsuchihashi, 1970a,b; Tsuchihashi, 1974; Williams, 1991). The importance of cheiloscopy is linked to the fact that the lip prints are unique to one person, except in monozygotic twins (Neville et al., 2002), like fingerprints and palatal rugae, the lip grooves are permanent throughout life (Tsuchihashi, 1974). It is possible to identify lip patterns as early as the 6th week of uterine life (Caldas et al., 2007; Koneru et al., 2013). The oily and moist secretions from sebaceous and salivary glands located at the vermillion border and subsequent mois- turization from the tongue enables the formation of a latent lip print whenever there is contact (Ball, 2002) and is likely to be encountered and should be suspected to be present on the scene of the crime of burglary, sexual assault, house tress-pass, homicide, rape, etc. Depending upon the scenario of/at the crime scene, lip prints may be found on various phys- ical evidences at the crime scene, such as shirt, handkerchief, tissue paper/wipes, cups, photographs, letters, glass, window panes, cutlery, fruit skin/peel, cigarette butts, clothing, and even biological materials such as skin (Kavitha et al., 2009; Vats et al., 2012). Lip prints are very useful in forensic investigations and are considered to be important forms of transfer evidence, and are analogous to fingerprints (Tsuchihashi, 1974). Apart from identification and evidential use, lip prints may also be used in detection work, being the source of tactical and criminal- istic information. Being unknowingly left at the scene of the crime, lip prints can directly and effectively be helpful in placing the suspect on the scene (Satyanarayana et al., 2011). A lip print at the scene of crime can be a basis for con- clusions as to the character of the event, the number of the people involved, sexes, cosmetics used, habits, occupational traits, and the pathological changes of lips themselves (Vahanwala and Parekh, 2000). If a complete match or identification is not possible, proper examination of lip prints may help in establishing other relative facts like sex identification of the donor, hence reducing the burden of the forensic examiner to half. The objective of this study is to determine predominant lip print pattern found among a central Indian population, to evaluate whether any sex difference exists and to study the permanence of the same over a 6 month duration. Please cite this article in press as: Kapoor, N., Badiye, A. A study of distribution, s Journal of Biological Sciences (2015), http://dx.doi.org/10.1016/j.sjbs.2015.01.014 2. Methodology 2.1. Sample The study comprised of 200 healthy individuals (100 males and 100 females), in the age group of 18–25 years and belonged to Marathi population of the Nagpur region of the Maharashtra state, India. Informed consent was obtained from all the subjects. 2.2. Inclusion criteria Only healthy subjects, free from any oral pathologies, inflam- mation, abnormalities or deformities such as cleft lip, cut marks, surgical scars or lesions of the lip were included in the study. 2.3. Recording the lip prints Recording of the data is an extremely important step for the success of this study; still digital photography was used, as the mobile nature of the human lips can affect the accuracy of the lip print impressions even with slight variations in the strength or the direction of the pressure applied (Tsuchihashi, 1974). The subjects were made to stand erect with the head positioned in Frankfurt plane. From a fixed dis- tance, lips of volunteers in ‘natural condition’ (without the application of lipstick, lip fillers, lip gloss or any other cosmetic product) were photographed twice using a digital camera (Nikon D3100-14.2 MP-AF-S NIKKOR 18–55 mm lens kit). This method is relatively easier and involved nil physical con- tact with the volunteers in terms of application of lip gloss or lipstick as previously used and suggested by others, which can be quite laborious and unhygienic (if the same lipstick is used for all the subjects). 2.4. Classification used In this study, we followed (Fig. 1) the classification of patterns of the lines on the lips proposed by Suzuki and Tsuchihashi (Suzuki and Tsuchihashi, 1971; Tsuchihashi, 1974). � Type I: Long vertical (Clear-cut vertical grooves that run across the lips). � Type I0: Short vertical (Partial length groove of type I). � Type II: Branched grooves (Branching Y-shaped pattern). � Type III: Intersected grooves (Criss-cross/’x’ pattern grooves). � Type IV: Reticular pattern (Grooves that forms rectangular shape). � Type V: Mixed/Indefinite (Grooves that do not fall into any of the above categories, combination of two or more pat- terns and/or cannot be differentiated morphologically/ undetermined). 2.5. Examination of the prints After transferring the photographs of the lips on a computer, lip prints (Fig. 2) were divided into four quadrants namely ex differences and stability of lip print patterns in an Indian population. Saudi http://dx.doi.org/10.1016/j.sjbs.2015.01.014 Lip Print Type Type I Long Vertical Type I’ Short Vertical Type II Branched Type III Intersecting Type IV Reticulate Type V Indefinite/Mixed Figure 1 Photographs showing all the patterns of the lip prints followed/observed in this study. A B CD Figure 2 Lip print divided clockwise in four quadrants namely A, B, C and D. Table 1 Percentage distribution of lip patterns in the Marathi population. Lip pattern types All quadrants (A + B + C + D) Percentage Long vertical (Type I) 30.63% Short vertical (Type I0) 1.88% Branched (Type II) 16.50% Intersecting (Type III) 25.38% Reticulate (Type IV) 8.63% Mixed/Indefinite (Type V) 17% Total 100 Study of lip print patterns in an Indian population 3 A, B, C, D moving clockwise, starting from the left side of the upper lip to the left side of the lower lip. Quadrants were man- ually classified twice by both authors independently, so as to assess the inter- and intra-rater reliability of the observations. The same criteria of inclusion and exclusion; procedure for recording the prints; classification and same method of exam- Please cite this article in press as: Kapoor, N., Badiye, A. A study of distribution, s Journal of Biological Sciences (2015), http://dx.doi.org/10.1016/j.sjbs.2015.01.014 ination were repeated for the same individuals after 6 months. The older photographs were compared with the recent ones by the same observers. 2.6. Statistical analysis The obtained results were statistically analyzed using the Chi-Square test, wherein a value of p < 0.05 was considered as significant. Kappa (K) value was also calculated to check the inter-observer and intra-observer agreement strength. 3. Result and discussion The division of lip prints into four quadrants was in accordance to Tsuchihashi (1974), Gondivkar et al. (2009), Saraswathi et al. (2009), ElDomiaty et al. (2010), Satyanarayana et al. (2011), Gupta et al. (2011), Venkatesh and David (2011), Prabhu et al. (2012), Koneru et al. (2013) and Prabhu et al. (2013). In the present study, overall, Type I (long vertical) was the most frequently observed pattern (Table 1) in the examined subjects of the Marathi population of the Nagpur region of the Maharashtra state in India. The result is in accordance to results of the studies conducted by Koneru et al. (2013) on Kerala and Manipur population and by Vahanwala and Parekh (2000) on the Mumbai population, who also found the Type I pattern to be most predominant, while Type I0 was observed to be the least common type. Type I (long vertical) and Type III (intersecting) lip print patterns were found to be most commonly found in males and females respectively. In male lip prints, the order of appearance of patterns were Type I > Type V > Type II > Type III > Type IV > Type I0 (least common). In female lip prints, the order of appearance of patterns were Type III > Type I > Type II > Type IV > Type V > Type I0 (least common) shown in Table 2 and Fig. 3. Quadrant wise distributions of lip print patterns are shown in Table 3. Some interesting points to be noted as they appear here are that the upper lips of males and females have about the same frequency of Type V prints, while the lower lips are vastly different (66 Type V lower lip patterns in males; 2 in females). Thus, the difference in the frequency of Type V between males and females is coming almost entirely from the lower lip, and is a much stronger sex difference than found when combining upper and lower lip frequencies. Other upper/ lower lip differences are seen in Type II patterns (no sex differ- ences here), and type I and type IV, females only. ex differences and stability of lip print patterns in an Indian population. Saudi http://dx.doi.org/10.1016/j.sjbs.2015.01.014 Table 4 Statistical analysis result (applying the Chi-Square test). Statistical analysis Lip print patterns: males Vs females Quadrant A * Quadrant B * v2 17.1 14.9 df 5 5 Probability 0.004 0.011 v2 = Value of Chi-Square, df = Degree of freedom. * Significant at p < 0.05. Table 2 Distribution of patterns among male and females. Pattern Distribution of patterns Male Female n % n % Long vertical (Type I) 119 29.75 126 31.50 Short vertical (Type I0) 14 3.5 1 0.25 Branched (Type II) 75 18.75 57 14.25 Intersecting (Type III) 62 15.5 141 35.75 Reticulate (Type IV) 27 6.75 42 10.5 Mixed/Indefinite (Type V) 103 25.75 33 8.25 Total 400 100 400 100 0 5 10 15 20 25 30 35 40 (Type I) (Type I’) (Type II) (Type III) (Type IV) (Type V) Male Female Figure 3 Percentage distribution of lip print patterns in the studied group. Table 3 Quadrant wise distribution of patterns in lip prints of mal Pattern type Male A B C Long vertical (Type I) 20 23 43 Short vertical (Type I0) 5 4 2 Branched (Type II) 34 34 2 Intersecting (Type III) 12 16 15 Reticulate (Type IV) 9 6 8 Mixed/Indefinite (Type V) 20 17 30 Total 100 100 100 A, B, C, D = Quadrant. 4 N. Kapoor, A. Badiye Please cite this article in press as: Kapoor, N., Badiye, A. A study of distribution, s Journal of Biological Sciences (2015), http://dx.doi.org/10.1016/j.sjbs.2015.01.014 Statistical analysis, applying the Chi-Square test, shows sig- nificant difference (p < 0.05) between lip print patterns in males and females in all quadrants individually as well as com- bined (Table 4). The result is in accordance with the results obtained by Kumar et al. (2012) in the Pondicherry popula- tion, Vats et al. (2012), Dwivedi et al. (2013) and Koneru et al. (2013) in Kerala and Manipuri populations, but is in con- trast to the results obtained by Sivapathasundharam et al. (2001), Saraswathi et al. (2009), Sandhu et al. (2012), Prabhu et al. (2013) and Verghese et al. (2010) who did not find any statistically significant differences. Statistically insignificant (p > 0.05) inter-observer and intra-observer variability was observed in assessing the mor- phologic pattern of the lips in all the four quadrants by both authors. The calculated Kappa (K) values showed that the strength of agreement between both the observers was ‘good’ to ‘very good’. Considered an environmental factor, peeling off the super- ficial layers of the skin of the lips was not seen in the present study, which is in contrast to the study by ElDomiaty et al. (2010) who observed it as a common feature of the lips in their study and explained it as a probable result of the dry weather in the study area, which dried the lips and made the people accustomed to bite away the dried skin. However, they also noted that, this did not mask the pattern of the lip print as it appeared after taking the print many times. This phenomenon could help a great deal in identification of subjects as the lip pattern does not change due to differences in climate or any illness present around the mouth (Tsuchihashi, 1974). No two lip prints showed the exactly same pattern in our study, which is in accordance to the detailed study by Tsuchihashi (1974) on 1364 Japanese subjects (757 males and 607 females) where no lip print showed the same pattern in Quadrant C * Quadrant D * All quadrants * 46.8 47.1 84.0 5 5 5 0.000 0.000 0.000 es and females. Female D A B C D 33 12 15 49 50 3 0 0 0 1 5 28 25 2 2 19 27 28 45 41 4 18 16 3 5 36 15 16 1 1 100 100 100 100 100 ex differences and stability of lip print patterns in an Indian population. Saudi http://dx.doi.org/10.1016/j.sjbs.2015.01.014 Study of lip print patterns in an Indian population 5 the investigation. He also observed that although the lip print patterns of the uniovular twins are in duplicate, in detail no two of them were exactly identical. The lip prints of the studied individuals in our study remained unchanged even after 6 months, which is practically useful in a criminal search where the unchanged pattern even for short period (6 month) would be helpful (Tsuchihashi, 1974). These findings are in accordance with the results of (Tsuchihashi, 1974) who studied the lip prints of the same indi- viduals every month for three years to see whether the lip prints are permanent or not. None showed any change throughout this period. Similar results in terms of permanence were demonstrated in a very recent study by ElDomiaty et al., 2014 who investigated the stability of lip-print patterns in, Saudi females, over time to validate their secure use in civil and criminal investigations by analyzing and comparing the prints of the same subjects taken 3 years earlier. They also urged the need for similar studies on a larger sample size of both sexes to confirm the rate of stable lip-print patterns and to investigate the sex differences, so as to validate lip prints as a powerful forensic tool. 4. Conclusion The study revealed that lip print Type I (30.63%) and Type I0 (1.88%) were the most and least predominant (respectively) among the Marathi population of the Nagpur region of the Maharashtra state in India. In males Type I (29.75%) and in females Type III (35.75%) were found to be most prevalent and Type I0 least seen in both sexes. The statistically significant difference (p < 0.05) obtained in all the quadrants individually and taken together suggests the potential of usage in sex dis- crimination. The lip print patterns were also found to be stable over a period of 6 months. Further studies on similar grounds considering different populations should be done in order to create a comprehensive database so that the hidden potential of lip prints as an important source of information can be utilized optimally. Acknowledgement The authors are thankful to all the individuals who donated their prints for the study. We also express our deepest grati- tude to Dr. Anjali M. Rahatgaonkar, Director, Govt. Institute of Forensic Science, Nagpur, for her constant support, motiva- tion, encouragement and appreciation for research activities and submitting the research to international journals of repute. We also express our deepest regards to the anonymous review- ers for their valuable suggestions which have contributed to the enhancement of the quality of this paper. References Ball, J., 2002. The current status of lip prints and their use for identification. J. Forensic Odontostomatol. 20, 43–46. Caldas, I.M., Magalhaes, T., Afonso, A., 2007. Establishing identity using cheiloscopy and palatoscopy. Forensic Sci. Int. 165 (1), 1–9. Dwivedi, N., Agarwal, A., Kashyap, B., Raj, V., Chandra, S., 2013. Latent lip print development and its role in suspect identification. J. Forensic Dent. Sci. 5, 22–27. Please cite this article in press as: Kapoor, N., Badiye, A. A study of distribution, s Journal of Biological Sciences (2015), http://dx.doi.org/10.1016/j.sjbs.2015.01.014 ElDomiaty, M.A., Al-gaidi, S.A., Elayat, A.A., Safwat, M.D., Galal, S.A., 2010. Morphological patterns of lip prints in Saudi Arabia at Almadinah Almonawarah province. Forensic Sci. Int. 200 (179), 1–9. ElDomiaty, M.A., Anwar, Rasha I., Algaidi, Sami A., 2014. Stability of lip-print patterns: a longitudinal study of Saudi females. J. Forensic Legal Med. 22, 154–158. Gondivkar, S.M., Indurkar, A., Degwekar, S., Bhowate, R., 2009. Cheiloscopy for sex determination. J. Forensic Dent. Sci. 1, 56–60. Gupta, S., Gupta, K., Gupta, O.P., 2011. A study of morphological patterns of lip prints in relation to gender of North Indian population. J. Oral Biol. Craniofac. Res. 1, 12–16. Kasprzak, J., 2000. Cheiloscopy. In: Siegel, J.A., Saukko, P.J., Knupfer, G.C. (Eds.), Encyclopaedia of Forensic Sciences, 1. Academic Press, London, pp. 358–361. Kasprzak, J., 1990. Possibilities of cheiloscopy. Forensic Sci. Int. 46, 145–151. Kavitha, Einstein, Sivapathasundharam, B., Saraswathi, T.R., 2009. Limitations in forensic odontology. J. Forensic Odontol. 1, 8–10. Koneru, A., Surekha, R., Nellithady, G.S., Vanishree, M., Ramesh, D., Patil, R.S., 2013. Comparison of lip prints in two different populations of India: reflections based on a preliminary examina- tion. J. Forensic Dent. Sci. 5, 11–15. Kumar, G.S., Vezhavendhan, N., Vendhan, P., 2012. A study of lip prints among Pondicherry population. J. Forensic Dent. Sci. 4, 84–87. Molano, M.A., Gil, J.H., Jaramillo, J.A., Ruiz, S.M., 2002. Estudio queiloscó pico en estudiantes de la facultad de odontologı́a de la Universidad de Antı́oquia. Rev. Fac. Odontol. Univ. Antioq. 14 (1), 26–33. Neville, B., Damm, D., Allen, C., Bouquot, J., 2002. Oral and Maxillofacial Pathology, second ed. WB Saunders Company, Philadelphia, pp. 763–774. Prabhu, R.V., Dinkar, A., Prabhu, V., 2012. A study of lip print pattern in Goan dental students – a digital approach. J. Forensic Legal Med. 19, 390–395. Prabhu, R.V., Dinkar, A., Prabhu, V., 2013. Digital method for lip print analysis: a new approach. J. Forensic Dent. Sci. 5, 96–105. Rajendran, R., Sivapathasundharam, B., 2006. Shafer’s Textbook of Oral Pathology, sixth ed. Elsevier, New Delhi, India, pp. 896–897. Reddy, L., Reddy, Vamsi Krishna, 2011. Lip prints: an overview in forensic dentistry. JADR II. Sandhu, S.V., Bansal, H., Monga, P., Bhandari, R., 2012. Study of lip print pattern in a Punjabi population. J. Forensic Dent. Sci. 4, 24–28. Saraswathi, T.R., Mishra, G., Ranganathan, K., 2009. Study of lip prints. J. Forensic Dent. Sci. 1, 28–31. Satyanarayana, N.K., Prabhu, A., Nargund, R., 2011. Forensic odontology: cheiloscopy. Hong Kong Dent. J. 8, 25–28. Shafer, Hine, Levy, 2009. Shafer’s Textbook of Oral Pathology, sixth ed. Elsevier, Noida, India, pp. 871–897. Sharma, P., Saxena, S., Rathod, V., 2009. Comparative reliability of cheiloscopy and palatoscopy in human identification. Indian J. Dent. Res. 20 (4), 453–457. Sivapathasundharam, B., Prakash, P.A., Sivakumar, G., 2001. Lip prints (cheiloscopy). Indian Dent. Res. 12, 234–237. Suzuki, K., Tsuchihashi, Y., 1971. A new attempt of personal identification by means of lip print. Can. Soc. Forensic Sci. J. 4, 154–158. Suzuki, K., Tsuchihashi, Y., 1970a. New attempt of personal identi- fication by means of lip print. JADA 42, 8–9. Suzuki, K., Tsuchihashi, Y., 1970b. Personal identification by means of lip prints. J. Forensic Med. 17, 52–57. Thomas, C.J., VanWyk, C.W., 1988. The palatal rugae in identifica- tion. J. Forensic Odontostomatol. 6, 21–27. ex differences and stability of lip print patterns in an Indian population. Saudi http://refhub.elsevier.com/S1319-562X(15)00015-7/h0005 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0005 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0010 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0010 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0015 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0015 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0015 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0025 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0025 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0025 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0025 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0030 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0030 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0030 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0035 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0035 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0040 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0040 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0040 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0045 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0045 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0045 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0050 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0050 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0020 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0020 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0020 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0055 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0055 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0055 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0055 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0060 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0060 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0060 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0065 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0065 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0065 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0065 http://refhub.elsevier.com/S1319-562X(15)00015-7/h9000 http://refhub.elsevier.com/S1319-562X(15)00015-7/h9000 http://refhub.elsevier.com/S1319-562X(15)00015-7/h9000 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0075 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0075 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0075 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0080 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0080 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0085 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0085 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0090 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0090 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0095 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0095 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0095 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0100 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0100 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0105 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0105 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0110 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0110 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0115 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0115 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0115 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0120 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0120 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0125 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0125 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0125 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0130 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0130 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0135 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0135 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0140 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0140 http://dx.doi.org/10.1016/j.sjbs.2015.01.014 6 N. Kapoor, A. Badiye Tsuchihashi, Y., 1974. Studies on personal identification by means of lip prints. Forensic Sci. Int. 3, 233–248. Vahanwala, S.P., Parekh, D.K., 2000. Study of lip prints as an aid to forensic methodology. J. Indian Dent. Assoc. 71, 269–271. Vats, Y., Dhall, J.K., Kapoor, A.K., 2012. Gender variation in morphological patterns of lip prints among some north Indian populations. J. Forensic Dent. Sci. 4, 19–23. Venkatesh, R., David, M.P., 2011. Cheiloscopy: an aid for personal identification. J. Forensic Dent. Sci. 3, 67–70. Please cite this article in press as: Kapoor, N., Badiye, A. A study of distribution, s Journal of Biological Sciences (2015), http://dx.doi.org/10.1016/j.sjbs.2015.01.014 Verghese, A.J., Somasekar, M., Umesh, B.R., 2010. A study of lip print types among the people of Kerala. J Indian Acad. Forensic Med. 32 (1), 6–7. Warren, H., 1976. Look, ask, listen and note. In: Henry, K. (Ed.), Dental Identification and Forensic Odontology. Henry Kimpton Publishers, London, pp. 22–23. Williams, T.R., 1991. Lip prints – another means of identification. J. Forensic Ident. 41, 190–194. ex differences and stability of lip print patterns in an Indian population. Saudi http://refhub.elsevier.com/S1319-562X(15)00015-7/h0145 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0145 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0150 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0150 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0155 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0155 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0155 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0160 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0160 http://refhub.elsevier.com/S1319-562X(15)00015-7/h9005 http://refhub.elsevier.com/S1319-562X(15)00015-7/h9005 http://refhub.elsevier.com/S1319-562X(15)00015-7/h9005 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0170 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0170 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0170 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0175 http://refhub.elsevier.com/S1319-562X(15)00015-7/h0175 http://dx.doi.org/10.1016/j.sjbs.2015.01.014 A study of distribution, sex differences and stability of lip print patterns in an Indian population 1 Introduction 2 Methodology 2.1 Sample 2.2 Inclusion criteria 2.3 Recording the lip prints 2.4 Classification used 2.5 Examination of the prints 2.6 Statistical analysis 3 Result and discussion 4 Conclusion Acknowledgement References