Impact of Fungiform Papillae Count on Taste Perception and Different Methods of Taste Assessment and their Clinical Applications A comprehensive review *Asim M. Khan,1 Saqib Ali,1 Reshma V. Jameela,1 Muhaseena Muhamood,1 Maryam F. Haqh2 Sultan Qaboos University Med J, August 2019, Vol. 19, Iss. 3, pp. e184–191, Epub. 5 Nov 19 Submitted 23 Dec 18 Revision Req. 6 Feb 19; Revision Recd. 10 Mar 19 Accepted 12 Apr 19 1Department of Biomedical Dental Science, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia; 2Department of Pedodontics & Preventive Dentistry, Oxford Dental College & Hospital, Bangalore, India *Corresponding Author’s e-mail: amkhan@iau.edu.sa Fungiform papillae are mushroom-shaped structures located on the dorsum of the anterior two-thirds of the tongue.1–3 Due to their rich capillary network, larger size and patchy distribution, fungiform papillae can be identified as reddish dots that contrast to the smaller and more numerous filiform papillae. According to cadaveric data, each individual has an estimated 200 fungiform papillae which are denser on the tip of the tongue in comparison to the middle region (29 versus 7–8 papillae per cm2).2,3 Each fungiform papilla carries between 0–20 taste buds, with an average of 2–4 buds; however, not all fungiform papillae contain taste buds and function as taste receptors at a given time. There are approximately 2,500 taste buds in the fungiform papillae of the anterior two- thirds of the tongue, although this has been reported to vary by approximately 18-fold between individuals.1–3 Based on their morphology, fungiform papillae are classified into four types that represent varying degrees of pathological severity.4 Type 1 fungiform papillae are the healthiest and are egg-shaped or long and elliptical and devoid of surface thickness, while type 2 are slightly thicker. Type 3 are thick and have an irregular surface while type 4—which represent the most pathological state—are flat and have an atrophic surface.4 Most studies investigating taste function utilise fungiform papillae count as a tool to indicate either decreased or increased taste sensitivity.5–7 To this end, fungiform papillae count has been correlated with various electrogustometric and chemogustometric thre- sholds. This review provides an overview of fungiform papillae, different methods for their quantification and the factors affecting these structures. In addition, various methods for recording taste sensation and their clinical applications are also discussed. review أثر َعّد احلليمات الكميئة على إدراك حاسَُّة الذَّوق والطرق املختلفة لتقييم الذَّوق، وتطبيقاهتا السريرية مراجعة شاملة عا�صم م�صطفى خان، ثاقب علي، ر�صما جميلة، حما�صنا حممود، مرمي ف�صيحة حق abstract: Fungiform papillae are raised lingual structures which contain taste buds and thus play an important role in taste perception. These structures vary in number due to their relative sensitivity to a range of systemic and local factors which affect the dorsum of the tongue. Taste sensation can be measured using both chemical and electrical methods; however, the number of fungiform papillae has a direct effect on chemogustometric and electrogustometric values during evaluation. This review provides a general overview of fungiform papillae, their quantification methods and the various factors which may affect these structures. In addition, numerous methods of recording taste sensation and their clinical applications are highlighted. Keywords: Sensation; Taste; Taste Perception; Tongue; Taste Buds; Investigative Techniques. ُة �لذَّوق. وتتفاوت امللخ�ص: �حلليمات �لكمئية هي بنيات ل�صينية مرتفعة حتتوي على بر�عم �لذوق، ولهذ� فهي توؤدي دور� مهما يف �إدر�ك حا�صَّ �أعد�د تلك �لبنيات ن�صبة للتفاوت يف ح�صا�صيتها �لن�صبية جتاه طيف من �لعو�مل �جلهازية و�ملحلية �ملوؤثرة على ظهر �لل�صان. وميكن قيا�س ُة �لذَّوق �ملقا�صة كيميائيا، و كهربائيا، يف ُة �لذَّوق بطرق كيميائية وكهربائية. �إال �أن لَعّد �حلليمات �لكميئة �أثر� مبا�رص� على قيم حا�صَّ حا�صَّ غ�صون فرتة �لتقييم. وتهدف هذه املراجعة إىل تقديم ��صتعر��س �صامل للحليمات �لكميئة، وطرق عدها، و�لعو�مل �ملختلفة �لتي توؤثر على تركيبها. باالإ�صافة لذلك يتطرق �ملقال للطرق �ملختلفة �لتي ميكن بها قيا�س حا�صة �لذَّوق، واستعراضتطبيقاتها �ل�رصيرية. ُة �لذَّوق؛ ال�صان؛ بر�عم �لذوق؛ طرق �ال�صتق�صاء. وق؛ �إدر�ك حا�صَّ الكلمات املفتاحية: ح�س؛ ذَّ This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License. https://doi.org/10.18295/squmj.2019.19.03.003 https://creativecommons.org/licenses/by-nd/4.0/ Asim M. Khan, Saqib Ali, Reshma V. Jameela, Muhaseena Muhamood and Maryam F. Haqh Review | e185 Taste Receptors The average human has a total of approximately 10,000 taste buds.8 These are located in the mucosa of the epiglottis, palate and pharynx as well as in fungiform and circumvallate papillae on the tongue. Fungiform papillae are most numerous around the tip of the tongue, whereas circumvallate papillae are arranged in a ‘V’ pattern on the posterior third of the tongue immediately anterior to the sulcus terminalis.8 The taste buds on the fungiform papilla are mostly embedded on the surface of the papilla. In contrast, the larger circumvallate papillae have approximately 100 taste buds located in the side walls.8 The minute conical filiform papillae spread over the remaining tongue surface lack taste buds. Taste buds are composed of ovoid bodies measuring 50–70 µm.8 Each bud consists of basal cells as well as type 1, 2 and 3 cells. Type 1 and 2 cells (i.e. sustentacular cells) support type 3 cells (i.e. the main gustatory cells) and are connected with sensory nerve fibres.8 Type 3 cells open up in the oral cavity via an opening called the taste pore which contain microvilli projecting from the taste cells. The necks of all three cell types are connected to each other and to the surrounding epithelial cells by tight junctions, so that only the microvilli are exposed to fluids in the oral cavity [Figure 1A].9 Each taste bud is innervated by 50 nerve fibres which connect up to five taste buds each.8 The cells surrounding the taste buds give rise to basal cells which in turn differentiate into new taste receptor cells which replace the older ones every 10 days.8 Taste buds degen- erate and finally disappear when the sensory nerve supplying them is severed. However, if the nerve regen- erates, the surrounding cells become organised into new taste buds; this is attributed to the influence of a chem- ical inductive from the redeveloping nerve fibre.8 Taste Pathway The chorda tympani is a branch of the facial nerve which innervates the taste buds on the anterior two-thirds of the tongue, while the posterior third of the tongue and taste buds situated in other areas (i.e. the palate and epiglottis) are innervated via the glossopharyngeal and vagus nerves, respectively.10 Taste sensation is carried to the gustatory area of the nucleus tractus solitarius in the medulla oblongata by slow-conducting myelinated nerve fibres. From the nucleus tractus solitarius, the neurons relay into the medial lemniscus of the medulla oblongata.10 The axons of these second-order neurons then transmit directly to the ventral posteromedial nucleus of the thalamus where they travel via thalamic radiation to the anterior part of the ipsilateral insula which facil- itates conscious perception of taste and taste discrim- ination [Figure 1B].10 However, in some individuals, all or some of these second-order neurons may cross over to the contralateral side and synapse at the thalamus, projecting to the contralateral cerebral cortex, while the remaining fibres continue to project to the ipsilateral cerebral cortex, thus resulting in bilateral representation.11 Taste Sensation Previously, it was believed that different areas of the tongue were responsible for perceiving the five different categories of taste (sweet, salty, bitter, sour and umami).12 Figure 1: Annotated diagrams of (A) taste bud morphology and (B) the taste pathway. Figure 1A was modified and reproduced with permission from Gowthamarajan et al.9 Impact of Fungiform Papillae Count on Taste Perception and Different Methods of Taste Assessment and their Clinical Applications A comprehensive review e186 | SQU Medical Journal, August 2019, Volume 19, Issue 3 However, it is now understood that the process of con- verting different taste stimuli into signals is not restricted to different zones of the tongue. Flavours are identified as a result of a backdrop of complex chemical mechanisms which involve ionic exchange through specific channels and secondary messenger activity.8,13 This process is known as taste signal transduction. s u p e r ta s t e r p h e n o m e n o n Supertaster phenomenon refers to the existence of individuals who experience tastes with far greater intensity than normal, to the degree that they can perceive usually tasteless substances, such as phenylthiocarbamide (PTC) and propylthiouracil (PROP). This concept was first described by Fox in 1932 when it was noted that PTC was tasteless to some individuals, yet perceived as bitter by others.14 While assessing the PTC taste response of over 2,500 subjects, Fox found that 65% of individuals recorded the taste as bitter, while 28% described it as tasteless and the remaining 6% described the taste in other ways.14 In recent years, research has revealed that this phenomenon is genetic in nature.13,15 This has been attributed to varying genotypes of the taste receptor 2 (TAS2R) member 38 gene.15–17 McMahon investigated supertaster phenomenon by assessing PROP taste status and papillae count among subjects who were able to appreciate unsweetened grapefruit juice; the results showed increased fungiform papillae density among supertasters, with non-supertasters having the lowest mean papillae densities.17 However, more recent research has shown that no correlation exists between fungiform papillae density and differences in taste perception.18,19 Methods of Counting Fungiform Papillae d i g i ta l p h o t o g r a p h y Nasri-Heir et al. utilised digital photography to assess fungiform papillae count by using a camera with a resolution of at least five megapixels to photograph the tongue alongside a millimetre slide rule.6 Subsequently, using a computer, a grid was superimposed over the image and stretched to coincide with markings on the ruler in order to create 1 cm2-sized boxes. The papillae within these boxes were then counted and averaged to arrive at a mean value.6 This technique has also been supplemented with image analysis software [Figure 2].7 c o n ta c t e n d o s c o p y a n d s ta i n i n g Pavlos et al. evaluated fungiform papillae density using contact endoscopy and the application of a methylene blue stain.20 First, a contact technique without staining was performed to aid imaging of the subepithelial capillaries; subsequently, the stain was applied to the epithelia and taste pores using a 1 cm2-sized piece of filter paper placed around the tip of the tongue. The fungi- form papillae were easily distinguishable as they were lightly stained in comparison to the darker filiform papillae.20 f o o d c o l o u r i n g d y e Zhang et al. used food dye (brilliant blue FCF133) to stain the tip of the tongue.21 The dye was transferred to the tongue using a 6-mm circular piece of filter paper. The stained area was then photographed using a digital camera and the photographs subjected to analysis using Adobe Photoshop® software (Adobe Inc., San Jose, California, USA).21 An example of this technique is shown in Figure 3.19 d e n v e r pa p i l l a e p r o t o c o l Nuessle et al. proposed a standardised method known as the Denver papillae protocol to define and prioritise the characteristics of fungiform papillae.22 This method involved manually counting the number of papillae in a 10-mm circular section of the tongue stained with a blue-coloured dye. Using image analysis software and digital photography, the fungiform papillae were then characterised based on their shape, colour, size and height.22 Individual fungiform papilla were rejected from the count if they were amorphous, stained blue in comparison to their surroundings, less than 0.5 mm in length or if they were lower in height compared to the surface of the tongue floor or the adjacent papillae.22 a u t o m at e d d e t e c t i o n Eldeghaidy et al. reported a method to automatically detect fungiform papillae on the dorsum of the tongue in collaboration with MATLAB® image analysis software (MathWorks, Natick, Massachusetts, USA).18 The anterior 2 cm section of the dorsum of the tongue was automatically divided into eight regions by the software in which fungiform papillae were counted using various algorithms. However, one limitation of Figure 2: Image of fungiform papillae quantification using digital photography and computer software. Reproduced with permission from Khan et al.7 Asim M. Khan, Saqib Ali, Reshma V. Jameela, Muhaseena Muhamood and Maryam F. Haqh Review | e187 this methodology is that the software may inaccurately assess the diameter of the papillae because the software considers all fungiform papillae to be exactly circular in shape.18 Discrepancies have therefore been noted when the diameters of fungiform papillae are validated manually.18,23 Factors Affecting Fungiform Papillae Count Nutrition and age are two major factors that affect the number of papillae present on the dorsum of the tongue.24,25 Certain nutrients such as vitamin B12 and folate are important to maintain an optimum balance between cell regeneration and deterioration;24 this is particularly important as the taste cells in the papillae have a high turnover rate.8 In addition, vitamin A deficiency may result in keratinisation and the loss of integrity of such cells in the epithelium. Zinc deficiency can also have similar consequences.24 Increasing age also slows down cellular regeneration, with 70% fewer taste buds recorded in individuals aged 70 years old compared to those aged 30 years.24 This also explains why many older individuals often have decreased taste sensitivity.25 Due to their high metabolic activity, cells forming filiform and fungiform papillae are also sensitive to enzyme, circulation or nutrient disturbances which can lead to atrophy. During atrophy, filiform papillae are more vulnerable to such disturbances compared to fungiform papillae; moreover, following atrophy, fungiform papillae regenerate faster in comparison to filiform papillae.24 Saito et al. reported that fungiform papillae atrophied among patients with normal pre- operative gustatory function after sectioning of the chorda tympani nerve; subsequently, the papillae reco- vered after regeneration or re-adaption of the nerve.26 Spielman et al. biopsied fungiform papillae from the dorsum of the tongue and reported papillae regener- ation at the same site after 40 days.27 Nasri-Heir et al. reported that the fungiform papillae count differed between patients with burning mouth syndrome and healthy controls (27.554 ± 2.122 papillae per cm2 versus 31.575 ± 3.112 papillae per cm2).6 Zhang et al. reported an inverse correlation between the number of fungiform papillae and taste thresholds using sucrose among young male subjects.21 Numerous other factors also affect the density of fungiform papillae, such as smoking.20 Various other potential causes of lingual papillae loss are listed in Table 1.28 Methods of Evaluating Taste Sensation The two primary methods of evaluating taste sensation are chemogustometry and electrogustometry. Figure 3: Photographs of tongue stained with brilliant blue FCF before (A) and after (B) fungiform papillae quantification. Reproduced with permission from Jilani et al.19 Table 1: Factors potentially resulting in lingual papillae loss28 Nutritional deficiencies Peripheral vascular diseases Local factors Therapeutic agents • Iron-deficiency anaemia • Plummer-Vinson syndrome • Pernicious anaemia • Anaemia associated with parasitic infections (e.g. ascariasis and bilharziasis) • Tropical sprue or coeliac disease • Chronic alcoholism • Vitamin B deficiency (especially vitamin B2, B6, B12, folic acid and nicotinic acid) • Diabetic angiopathy • Vasculitis in patients with SLE • Endarteritis obliterans • Syphilitic glossitis • Obliteration of the small blood vessels (e.g. in scleroderma or submucous fibrosis) • Localised vascular insufficiency in elderly patients • Frictional irritation to the tip and lateral borders of the tongue • Atrophic lichen planus • Epidermolysis bullosa or ulceration which heals with scarring • Long-standing xerostomia • Drugs that interfere with the growth and maturation of the epithelium (e.g. cyclosporine) • Drugs that induce candidosis (e.g. antibiotics and steroids) • Drugs that induce xerostomia (e.g. anticholinergic drugs and radiotherapy) SLE = systemic lupus erythematosus. Impact of Fungiform Papillae Count on Taste Perception and Different Methods of Taste Assessment and their Clinical Applications A comprehensive review e188 | SQU Medical Journal, August 2019, Volume 19, Issue 3 c h e m o g u s t o m e t r y Chemogustometry involves the application of chemical solutions to the oral mucosa; subsequently, the degree to which any of the five types of taste presents itself is evaluated by equating the taste with that of a reference material.29 Thus, taste detection thresholds are eval- uated by asking the subject to taste a particular sub- stance in various concentrations. This is done system- atically by either increasing or decreasing the dilution of the substance. Water is used as a control during the process, with the subject ideally being able to discriminate between water and the diluted test solution.29 In order to appreciate any differences in the taste intensity of the substance being tested, there should be at least a 30% difference in dilution between solutions. Bitterness is detected by the action of the bitter substance on TAS2R receptors, a type of G protein-linked receptor.16 Chemically, the threshold for bitterness is assessed using quinine hydrochloride at a dilution of 1 g in 2 L of water.23 Saltiness is evaluated by assessing the action of the salty substance on epithelial sodium channel (ENaC) receptors using a diluted solution of sodium chloride. Taste transduction is thus induced by the influx of sodium ions in the ENaC receptors which facilitates the release of glutamate to depolarise neurons. Sourness, measured using diluted hydrochloric acid, also acts via the ENaC receptors which allow the inflow of protons and triggers neurons.8,23 Sweetness is assessed using a diluted sweet sub- stance such as sucrose or artificial sweeteners like saccharin, which differ in taste due to their distinct chemical structures. Taste transduction occurs via another G protein receptor known as taste receptor 1. The final type of taste, umami, is savoury in nature. Umami taste is induced by the activation of meta- botropic glutamate receptor 4 as a result of stimulants such as monosodium glutamate. Inosine monophosphate and guanosine monophosphate act as agonists during umami taste induction.8,23 Taste strips and disks One method of chemogustometric evaluation involves presenting tastants to subjects in a clinical setting via soaked elongated strips or circular disks.30,31 These taste strips or disks are usually made up of pullulan (α-1,4- and α-1,6-glucan) which is also combined with the polymer hydroxypropyl methylcellulose. Such strips or disks are dissolvable in the oral cavity and do not need to be retrieved afterwards.30 The three-drop method Using this method, three drops of a chemical tasting solution are placed in the middle of the dorsum of the tongue, approximately 1.5 cm from the tip.31 One drop contains the actual tasting solution and the other two are distilled water drops which act as a solvent. The testing process usually starts with the lowest concent- ration, with the solution increasing in concentration until the subject’s tasting threshold is detected.31 Electroencephalography Event-related potentials refer to the electrical responses evoked in the brain when an individual is presented with a stimulus. As such, after applying chemical gustatory stimuli to the tongue, electroencephalography can show cortical brain activity associated with the taste sensation, along with its topographical distribution.32 Advantages and disadvantages Chemogustometry consists of using an array of chem- ical solutions in multiple concentrations to assess taste sensation. Some advantages of this method include the long shelf-life of the materials needed, the ease of administration, the rapidity of testing and the fact that this method allows for evaluation of each side of the tongue separately.31 However, this method is qualitative in nature and more cumbersome in a clinical setting in comparison to electrogustometry. e l e c t r o g u s t o m e t r y Electrogustometry quantifies taste and measures the threshold of taste sensation by passing a controlled current through the tongue using electrodes. As cathodal stimuli do not produce any significant recordable sen- sation, a weak anodal current is used.33 The stimulus is a constant direct current of predefined amplitude and duration. The taste perceived during electrogustometry is described as sour-metallic or ‘battery’-like and is attributed to the absorption of protons (or hydronium ions) released by the stimulus.33 In 1754, Sulzer first described the ‘ferro-sulphate’- like metallic taste which occurs when two dissimilar metals come into contact with the tongue.34 In 1955, Skouby invented the first electrogustometer based on taste thresholds determined by placing chemical solutions on the tongue.35 Subsequently, the measure- ment of taste using an electric stimulus was reported by Krarup in 1958.34 Over time, electrogustometers have evolved in terms of their design, electrode composition and size.33,35 Electrogustometry is now a viable clinical tool to estimate taste function, though this method is yet to be commonly used. When an electrode from an electrogustometer is placed on tongue, two types of sensations are induced—tingling and taste.33 These two sensations are conducted via different nerves, with taste perceived as a visceral sensation by the chorda tympani nerve while the tingling is a mechanical sensation conducted by the lingual nerve. Therefore, electrogustometry aids in differentiating between the chorda tympani and lingual Asim M. Khan, Saqib Ali, Reshma V. Jameela, Muhaseena Muhamood and Maryam F. Haqh Review | e189 nerves and is especially important in determining the integrity of the neural pathway.6,33 However, since electrogustometric taste threshold measurements are subjective, uniformity must be maintained in the environment/set-up of the test and the way the subject is trained to respond to the sensations. Advantages and disadvantages Electrogustometry is a quick and quantitative tool to assess taste threshold, particularly among patients with taste disorders such as hemiageusia and ageusia.32,36 Moreover, it allows for the evaluation of the most minute taste deficits, even in the absence of symptoms, and can be used to determine the topographical location of such deficits along the taste pathway and glossopharyngeal nerve. Furthermore, electrogustometry can also aid in determining patient prognosis.36 However, a major drawback of electrogustometry is that it is subjective and relies on feedback from the subject.36 It also cannot be used to investigate or diagnose symptoms commonly associated with certain taste disorders, such as heterogeusia and spontaneous dysgeusia. Finally, this technique cannot be used for patients with artificial pacemakers as electrical stimuli from the electrodes may cause interference with electrical signals from the pacemaker.36 Clinical Applications b u r n i n g m o u t h s y n d r o m e Braud et al. reported a significant association between electrogustometric values and pain intensity measured by visual analogue scale among patients with burning mouth syndrome, indicating a potent interaction between gustatory and nociceptive components among affected subjects.37 Nasri-Heir et al. also noted signif- icantly higher electrogustometric responses among patients with burning mouth syndrome in comparison to a normal control group.6 The researchers concluded that burning mouth syndrome is a neurodegenerative phenomenon with decreased chorda tympani activity.6 c a n c e r Ovesen et al. reported higher taste thresholds in subjects with small-cell lung, breast and ovarian cancer compared to controls with non-neoplastic disease.38 Moreover, taste thresholds decreased among those patients who responded to chemotherapy, suggesting that malignant disease has an effect on taste sensation. These findings indicate that electrogustometry could be a useful diag- nostic tool in neoplastic cancers.38 Epstein et al. found that patients with cancer devel- oped taste disorders (i.e. dysgeusia) as a result of other factors apart from pathology, such as chemotherapy treatment.39 This is because chemotherapy drugs are released into the saliva and adhere directly to the taste buds, causing altered taste perceptions and resulting in a metallic or chemical taste. As such, it is recommended that taste and olfactory electrogustometric evaluations be made mandatory for all patients undergoing cancer treatment.39 n e u r o l o g i c a l d i s e a s e s Dzaman et al. reported that 13 out of 35 subjects with nasal polyps had increased taste and olfactory thresholds as assessed by electrogustometry compared to controls.40 Deeb et al. reported a deficit in electrogustometric thresholds among subjects with Parkinson’s disease, indicative of disease severity.41 p o s t o p e r at i v e pat i e n t s Doty et al. assessed the impact of factors such as age and gender on taste perception among individuals under- going chorda tympani nerve resection.42,43 Taste assess- ment was done in different regions of the tongue using filter paper soaked in tastants such as sucrose, sodium chloride and caffeine; in addition, the patients were subjected to electrical stimuli via electrogustometry. The researchers reported a deterioration in taste sensiti- vity commencing in middle age and progressively reducing after 50 years of age.42,43 This decline in taste sensitivity occurred for all stimuli at the anterior part of the tongue, with chorda tympani nerve resection resulting in taste deficits on the same side as well as the middle portion of the tongue.42,43 Boucher et al. investigated taste defects by electro- gustometry in patients with severed afferent connections caused by dental treatment.44 Higher electrogustometric thresholds were recorded in subjects with more than seven deafferented teeth compared to those with fewer deafferented teeth, with a significant direct correlation between electrogustometric thresholds and the number of deafferented teeth, regardless of age.44 Similarly, Michael et al. found a greater prevalence of electro- gustometric taste changes among patients following middle-ear surgery; this was ascribed to damage caused by the distention and, to a lesser degree, severance of the chorda tympani nerve.45 s m o k i n g Depressed or altered taste sensation has been reported among chronic smokers.46,47 Smoke from burning tobacco includes a variety of irritants, oncogenic particles such as tar and lead, as well as other poisonous substances such as carbon monoxide and nicotine. These not only topically affect taste receptors cells and impede the normal mechanism of taste conduction Impact of Fungiform Papillae Count on Taste Perception and Different Methods of Taste Assessment and their Clinical Applications A comprehensive review e190 | SQU Medical Journal, August 2019, Volume 19, Issue 3 but also affect the neurological transmission of taste sensations.46 The effects of tobacco on taste thresholds depend upon the individual’s susceptibility, the quantity and frequency of use and the age of the individual when they started smoking. Using a modified form of electro- gustometry, Khan et al. reported significantly lower fungiform papillae counts and greater electrical taste thresholds in smokers compared to non-smokers.7 Moreover, in a follow-up study of smokers before and after quitting, Chéruel et al. found that smoking cessation lead to a recovery in taste sensitivity.46 How- ever, the time required to regain taste functionality depended on the susceptibility of the region of the tongue that had been affected. The researchers advoc- ated for the use of electrogustometry as a method of motivating chronic smokers to stop smoking.46 Yekta et al. studied somatosensory function in the mucosa of the tongue.47 Subjects were tested bilaterally in tongue regions innervated by the lingual nerves according to sensory heat, pain and mechanical detection thresholds. Increased heat thresholds were reported in smokers in comparison to non-smokers; this was attributed to damage caused by smoking to the myelinated Aδ- and C-fibres of the tongue.47 Conclusion Fungiform papillae density offers valuable information regarding an individual’s taste perception and taste sensation thresholds, with both chemical and electrical tools available for quantification purposes. However, as chemogustometry is a mostly qualitative method of determining taste sensitivity and requires a complex array of chemical solutions, it can be cumbersome in a clinical setting. In contrast, electrogustometry is a quick and quantitative tool and has a wide range of clinical applications, including for patients with taste disorders, burning mouth syndrome and neoplastic cancers as well as for smoking cessation purposes. References 1. Miller IJ Jr, Bartoshuk LM. Taste perception, taste bud distribution, and spatial relationships. 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