id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_dyh7wdlzergmfamc2ynymotk2a	Andjin Siegenthaler	Quantifying pigment cover to assess variation in animal colouration	2017.0	9	.pdf	application/pdf	6405	845	67	illustrate a freely available and user-friendly method to quantify pigment cover (PiC) with high precision and low effort using digital images, where the foreground (i.e. pigments in chromatophores) can be detected and separated from the background. Images of the brown shrimp, Crangon crangon, were used to compare PiC with the traditional Chromatophore Index Results indicate that PiC outcompetes CI for pigment detection and transparency measures in terms of speed, accuracy The proposed methodology provides researchers with a useful tool to answer essential physiological, behavioural and evolutionary questions on animal colouration in a wide range of species. achieved with the 'Color Threshold' function (Fig. 1B), which segments 24-bit RGB images based on pixel values (see the ImageJ 'Saturation' and 'Brightness' scroll bar in the colour threshold settings window (Fig. 1B) until the whole area covered by the pigment(s) of interest is selected [44, 49].	./cache/work_dyh7wdlzergmfamc2ynymotk2a.pdf	./txt/work_dyh7wdlzergmfamc2ynymotk2a.txt