id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-345254-glm2dxhh	Hwang, Mihyun	Distinct CD4 T-cell effects on primary versus recall CD8 T-cell responses during viral encephalomyelitis	2015-02-13		.txt	text/plain	7372	372	54	By contrast, unhelped memory CD8 T cells mounted poor recall responses when transferred into CD4 T-cell-sufficient mice and could not be sustained in the CNS, despite efficient virus control. Donor mice were treated with anti-mouse CD4 or control mAb at day À2 and 0 relative to intraperitoneal immunization for comparative analysis of 'unhelped' versus 'helped' CD8 T cells. As T cells are the primary mediators of JHMV control in the CNS during the first 14 days p.i. 29, 30 the inability of CD4-depleted mice to reduce viral load suggested impaired CD8 T-cell recruitment or function. 15 We therefore examined potential defects in effector functions of brain-derived unhelped virus-specific CD8 T cells by measurement of ex vivo cytolytic activity and IFN-c expression. To generate unhelped or helped donor memory CD8 T cells, Thy-1.1 mice were either treated with anti-CD4 or control mAb before JHMV immunization.	./cache/cord-345254-glm2dxhh.txt	./txt/cord-345254-glm2dxhh.txt