id author title date pages extension mime words sentences flesch summary cache txt cord-319999-cpdpsg3i Zheng, Xiaotian Macrolide-Resistant Mycoplasma pneumoniae, United States 2015-08-17 .txt text/plain 1268 67 43 Testing for 23S rRNA mutations conferring macrolide resistance was performed on original specimens by real-time PCR melt curve analysis (6) and confirmed by DNA sequencing at the Lurie Children's Hospital (Chicago). We found a 23S rRNA point mutation A2063G known to confer macrolide resistance in 10 (10.9%) of 91 specimens by direct real-time PCR with melting curve analysis. Our finding of high-level macrolide resistance in 13.2% of specimens from all 6 centers throughout a broad geographic area in the United States proves this problem has emerged in all regions of the nation and might increase over time, as it has in other countries. Increased macrolide resistance of Mycoplasma pneumoniae in France directly detected in clinical specimens by real-time PCR and melting curve analysis Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR and high-resolution melt analysis Investigations of Mycoplasma pneumoniae infections in the United States: trends in molecular typing and macrolide resistance from 2006 to 2013 ./cache/cord-319999-cpdpsg3i.txt ./txt/cord-319999-cpdpsg3i.txt