id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300808-fgdyzzty	Tan, Joshua	A LAIR-1 insertion generates broadly reactive antibodies against malaria variant antigens	2015-12-23		.txt	text/plain	4599	231	50	In each of the two donors studied, the antibodies are produced by a single expanded B cell clone and carry distinct somatic mutations in the LAIR-1 domain that abolish binding to collagen and increase binding to infected erythrocytes. Plasma from adults (n = 557) living in a malaria-endemic region in Kilifi, Kenya, were initially tested in pools of five (Fig. 1b) and then individually for their capacity to agglutinate mixtures of erythrocytes infected with three culture-adapted Kenyan parasite isolates, each stained with a different DNA dye. The binding of the LAIR-1-containing antibodies to specific RIFINs was confirmed by the finding that MGD21 stained CHO cells transfected with the candidate antigens (PF3D7_1400600 and PF3D7_1040300), but not with irrelevant RIFINs that were similarly expressed (PF3D7_0100400 and PF3D7_0100200) or not detected (PF3D7_1100500) in 3D7-MGD21 + and 3D7-MGD21ghosts (Fig. 4c ).	./cache/cord-300808-fgdyzzty.txt	./txt/cord-300808-fgdyzzty.txt