id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274129-vaygaqe5	Cheng, Ming Soon	Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses	2015-08-15		.txt	text/plain	4669	221	49	We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 New Guinea C strain (including degenerative morphological changes, detachment, membrane degradation and death of host cells), were reflected by drastic decrease in impedance signal response detected as early as ~30 hours post-infection (hpi). In comparison to conventional inspection methods such as microscopy and plaque assay that rely on observable changes in the morphology and surface coverage of cells, the EIS technique offers a more promising platform for studying virus-host interactions based on the real-time measurement of CPE-induced impedance response (Cho et al., 2007) . Here we report an impedimetric cell-based biosensor constructed from poly-L-lysine (PLL)-modified screen-printed carbon electrode (SPCE) for real-time monitoring of DENV infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells.	./cache/cord-274129-vaygaqe5.txt	./txt/cord-274129-vaygaqe5.txt