id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-018469-3ip6566z	Wang, Denong	Carbohydrate Microarrays	2015-06-01		.txt	text/plain	7828	436	39	However, whether such a carbohydrate moiety preserves a B cell epitope or a potent antigenic determinant must be determined immunologically, including at least demonstration of its antibody-binding specificity and capacity in eliciting immune responses in vivo (Wang et al. Methods currently in use include non-covalent binding of underivatized carbohydrate antigens by passive adsorption on a chip, such as nitrocellulose-coated glass slides (Wang et al. For example, the method of nitrocellulose-based immobilization of carbohydratecontaining macromolecules, including polysaccharides, glycoproteins, and glycolipids, is suitable for the high-throughput construction of carbohydrate antigen microarrays (Wang et al. A high-precision robot designed to produce cDNA microarrays is utilized to spot carbohydrate antigens of various structural configurations onto a nitrocellulose-coated glass slide. Given the structural diversity of carbohydrate antigens, examining each antigen preparation to determine the efficacy of its immobilization in a given type of substrate and the surface display of the desired glyco-epitopes in a microarray assay is essential.	./cache/cord-018469-3ip6566z.txt	./txt/cord-018469-3ip6566z.txt