id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-308576-iw8oobbe	Wuxing, Dai	Expression and purification of SARS coronavirus membrane protein	2004		.txt	text/plain	1785	130	67	To construct a recombinant plasmid Pet23a-M, the gene encoding severe acute respiratory syndrome (SARS) coronavirus membrane protein was amplified by RT-PCR and cloned into the expression plasmid Pet23a. To screen and prepare effective SARS virus vaccine and diagnostic antigens, we designed a pair of primers to amplify the gene encoding SARS coronavirus membrane protein and cloned it into an expression plasmid Pet23a. Coli BI.21 ( D E 3 ) and induced by I P T G , Western-blot showed that the expressed 27 kD protein which was characterized by SDS-PAGE and purified by metal chelated chromatography could react with antibodies in sera of SARS patients during convalescence, demonstrating the recombinant protein possessed the biological activity of membrane protein.	./cache/cord-308576-iw8oobbe.txt	./txt/cord-308576-iw8oobbe.txt