id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-272467-8heg5iql	Armstrong, John	Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNA	2004-02-19		.txt	text/plain	2752	132	54	We have expressed the cloned cDNA for glycoproteins from two such viruses, the E1 protein of coronavirus, which buds in the Golgi region, and VP10 protein of rotavirus, which assembles in the endoplasmic reticulum. We are investigating two viral model proteins, one for the endoplasmic reticulum and one for the Golgi complex, with a view to determining the features of each molecule responsible for its correct localisation. RNAs prepared by this method for VPlO and E l were translated efficiencly in reticulocyte lysates, Xenopus oocytes, and cultured CVl cells (Figs. However, not all of the viral protein is restricted to the flattened cisternae of the Golgi complex; some at least is found in smooth membranes which are in the same region of the cell but are in fact continuous with the rough endoplasmic reticulum [ 12, 221 .	./cache/cord-272467-8heg5iql.txt	./txt/cord-272467-8heg5iql.txt