id	sid	tid	token	lemma	pos
cord-300796-rmjv56ia	1	1	key	key	NN
cord-300796-rmjv56ia	1	2	:	:	:
cord-300796-rmjv56ia	1	3	cord-300796-rmjv56ia	cord-300796-rmjv56ia	NNS
cord-300796-rmjv56ia	1	4	authors	author	NNS
cord-300796-rmjv56ia	1	5	:	:	:
cord-300796-rmjv56ia	2	1	nan	nan	NNP
cord-300796-rmjv56ia	2	2	title	title	NN
cord-300796-rmjv56ia	2	3	:	:	:
cord-300796-rmjv56ia	3	1	The	the	DT
cord-300796-rmjv56ia	3	2	signal	signal	JJ
cord-300796-rmjv56ia	3	3	sequence	sequence	NN
cord-300796-rmjv56ia	3	4	of	of	IN
cord-300796-rmjv56ia	3	5	the	the	DT
cord-300796-rmjv56ia	3	6	p62	p62	NN
cord-300796-rmjv56ia	3	7	protein	protein	NN
cord-300796-rmjv56ia	3	8	of	of	IN
cord-300796-rmjv56ia	3	9	Semliki	Semliki	NNP
cord-300796-rmjv56ia	3	10	Forest	Forest	NNP
cord-300796-rmjv56ia	3	11	virus	virus	NN
cord-300796-rmjv56ia	3	12	is	be	VBZ
cord-300796-rmjv56ia	3	13	involved	involve	VBN
cord-300796-rmjv56ia	3	14	in	in	IN
cord-300796-rmjv56ia	3	15	initiation	initiation	NN
cord-300796-rmjv56ia	3	16	but	but	CC
cord-300796-rmjv56ia	3	17	not	not	RB
cord-300796-rmjv56ia	3	18	in	in	IN
cord-300796-rmjv56ia	3	19	completing	complete	VBG
cord-300796-rmjv56ia	3	20	chain	chain	NN
cord-300796-rmjv56ia	3	21	translocation	translocation	NN
cord-300796-rmjv56ia	3	22	date	date	NN
cord-300796-rmjv56ia	3	23	:	:	:
cord-300796-rmjv56ia	3	24	1990	1990	CD
cord-300796-rmjv56ia	3	25	-	-	SYM
cord-300796-rmjv56ia	3	26	09	09	CD
cord-300796-rmjv56ia	3	27	-	-	HYPH
cord-300796-rmjv56ia	3	28	01	01	CD
cord-300796-rmjv56ia	3	29	journal	journal	NN
cord-300796-rmjv56ia	3	30	:	:	:
cord-300796-rmjv56ia	4	1	J	J	NNP
cord-300796-rmjv56ia	4	2	Cell	Cell	NNP
cord-300796-rmjv56ia	4	3	Biol	Biol	NNP
cord-300796-rmjv56ia	4	4	DOI	DOI	NNP
cord-300796-rmjv56ia	4	5	:	:	:
cord-300796-rmjv56ia	5	1	nan	nan	NNP
cord-300796-rmjv56ia	5	2	sha	sha	NNP
cord-300796-rmjv56ia	5	3	:	:	:
cord-300796-rmjv56ia	5	4	4b1b2e95b29b2fb4926c6c0191689f86537985bb	4b1b2e95b29b2fb4926c6c0191689f86537985bb	CD
cord-300796-rmjv56ia	5	5	doc_id	doc_id	CD
cord-300796-rmjv56ia	5	6	:	:	:
cord-300796-rmjv56ia	5	7	300796	300796	CD
cord-300796-rmjv56ia	5	8	cord_uid	cord_uid	NNS
cord-300796-rmjv56ia	5	9	:	:	:
cord-300796-rmjv56ia	5	10	rmjv56ia	rmjv56ia	NNP
cord-300796-rmjv56ia	6	1	So	so	RB
cord-300796-rmjv56ia	6	2	far	far	RB
cord-300796-rmjv56ia	6	3	it	-PRON-	PRP
cord-300796-rmjv56ia	6	4	has	have	VBZ
cord-300796-rmjv56ia	6	5	been	be	VBN
cord-300796-rmjv56ia	6	6	demonstrated	demonstrate	VBN
cord-300796-rmjv56ia	6	7	that	that	IN
cord-300796-rmjv56ia	6	8	the	the	DT
cord-300796-rmjv56ia	6	9	signal	signal	JJ
cord-300796-rmjv56ia	6	10	sequence	sequence	NN
cord-300796-rmjv56ia	6	11	of	of	IN
cord-300796-rmjv56ia	6	12	proteins	protein	NNS
cord-300796-rmjv56ia	6	13	which	which	WDT
cord-300796-rmjv56ia	6	14	are	be	VBP
cord-300796-rmjv56ia	6	15	made	make	VBN
cord-300796-rmjv56ia	6	16	at	at	IN
cord-300796-rmjv56ia	6	17	the	the	DT
cord-300796-rmjv56ia	6	18	ER	ER	NNP
cord-300796-rmjv56ia	6	19	functions	function	NNS
cord-300796-rmjv56ia	6	20	both	both	CC
cord-300796-rmjv56ia	6	21	at	at	IN
cord-300796-rmjv56ia	6	22	the	the	DT
cord-300796-rmjv56ia	6	23	level	level	NN
cord-300796-rmjv56ia	6	24	of	of	IN
cord-300796-rmjv56ia	6	25	protein	protein	NN
cord-300796-rmjv56ia	6	26	targeting	target	VBG
cord-300796-rmjv56ia	6	27	to	to	IN
cord-300796-rmjv56ia	6	28	the	the	DT
cord-300796-rmjv56ia	6	29	ER	ER	NNP
cord-300796-rmjv56ia	6	30	and	and	CC
cord-300796-rmjv56ia	6	31	in	in	IN
cord-300796-rmjv56ia	6	32	initiation	initiation	NN
cord-300796-rmjv56ia	6	33	of	of	IN
cord-300796-rmjv56ia	6	34	chain	chain	NN
cord-300796-rmjv56ia	6	35	translocation	translocation	NN
cord-300796-rmjv56ia	6	36	across	across	IN
cord-300796-rmjv56ia	6	37	the	the	DT
cord-300796-rmjv56ia	6	38	ER	ER	NNP
cord-300796-rmjv56ia	6	39	membrane	membrane	NN
cord-300796-rmjv56ia	6	40	.	.	.
cord-300796-rmjv56ia	7	1	However	however	RB
cord-300796-rmjv56ia	7	2	,	,	,
cord-300796-rmjv56ia	7	3	its	-PRON-	PRP$
cord-300796-rmjv56ia	7	4	possible	possible	JJ
cord-300796-rmjv56ia	7	5	role	role	NN
cord-300796-rmjv56ia	7	6	in	in	IN
cord-300796-rmjv56ia	7	7	completing	complete	VBG
cord-300796-rmjv56ia	7	8	the	the	DT
cord-300796-rmjv56ia	7	9	process	process	NN
cord-300796-rmjv56ia	7	10	of	of	IN
cord-300796-rmjv56ia	7	11	chain	chain	NN
cord-300796-rmjv56ia	7	12	transfer	transfer	NN
cord-300796-rmjv56ia	7	13	(	(	-LRB-
cord-300796-rmjv56ia	7	14	see	see	VB
cord-300796-rmjv56ia	7	15	Singer	Singer	NNP
cord-300796-rmjv56ia	7	16	,	,	,
cord-300796-rmjv56ia	7	17	S.	S.	NNP
cord-300796-rmjv56ia	7	18	J.	J.	NNP
cord-300796-rmjv56ia	7	19	,	,	,
cord-300796-rmjv56ia	7	20	P.	P.	NNP
cord-300796-rmjv56ia	7	21	A.	a.	NN
cord-300796-rmjv56ia	7	22	Maher	Maher	NNP
cord-300796-rmjv56ia	7	23	,	,	,
cord-300796-rmjv56ia	7	24	and	and	CC
cord-300796-rmjv56ia	7	25	M.	M.	NNP
cord-300796-rmjv56ia	7	26	P.	P.	NNP
cord-300796-rmjv56ia	7	27	Yaffe	Yaffe	NNP
cord-300796-rmjv56ia	7	28	.	.	.
cord-300796-rmjv56ia	8	1	Proc	proc	VB
cord-300796-rmjv56ia	8	2	.	.	.
cord-300796-rmjv56ia	9	1	Natl	Natl	NNP
cord-300796-rmjv56ia	9	2	.	.	.
cord-300796-rmjv56ia	10	1	Acad	Acad	NNP
cord-300796-rmjv56ia	10	2	.	.	.
cord-300796-rmjv56ia	11	1	Sci	Sci	NNP
cord-300796-rmjv56ia	11	2	.	.	NNP
cord-300796-rmjv56ia	11	3	USA	USA	NNP
cord-300796-rmjv56ia	11	4	.	.	.
cord-300796-rmjv56ia	11	5	1987	1987	CD
cord-300796-rmjv56ia	11	6	.	.	.
cord-300796-rmjv56ia	12	1	84:1015	84:1015	CD
cord-300796-rmjv56ia	12	2	-	-	SYM
cord-300796-rmjv56ia	12	3	1019	1019	CD
cord-300796-rmjv56ia	12	4	)	)	-RRB-
cord-300796-rmjv56ia	12	5	has	have	VBZ
cord-300796-rmjv56ia	12	6	remained	remain	VBN
cord-300796-rmjv56ia	12	7	elusive	elusive	JJ
cord-300796-rmjv56ia	12	8	.	.	.
cord-300796-rmjv56ia	13	1	In	in	IN
cord-300796-rmjv56ia	13	2	this	this	DT
cord-300796-rmjv56ia	13	3	work	work	NN
cord-300796-rmjv56ia	13	4	we	-PRON-	PRP
cord-300796-rmjv56ia	13	5	show	show	VBP
cord-300796-rmjv56ia	13	6	that	that	IN
cord-300796-rmjv56ia	13	7	the	the	DT
cord-300796-rmjv56ia	13	8	p62	p62	NN
cord-300796-rmjv56ia	13	9	protein	protein	NN
cord-300796-rmjv56ia	13	10	of	of	IN
cord-300796-rmjv56ia	13	11	Semliki	Semliki	NNP
cord-300796-rmjv56ia	13	12	Forest	Forest	NNP
cord-300796-rmjv56ia	13	13	virus	virus	NN
cord-300796-rmjv56ia	13	14	contains	contain	VBZ
cord-300796-rmjv56ia	13	15	an	an	DT
cord-300796-rmjv56ia	13	16	uncleaved	uncleaved	JJ
cord-300796-rmjv56ia	13	17	signal	signal	NN
cord-300796-rmjv56ia	13	18	sequence	sequence	NN
cord-300796-rmjv56ia	13	19	at	at	IN
cord-300796-rmjv56ia	13	20	its	-PRON-	PRP$
cord-300796-rmjv56ia	13	21	NH2-terminus	nh2-terminus	NN
cord-300796-rmjv56ia	13	22	and	and	CC
cord-300796-rmjv56ia	13	23	that	that	IN
cord-300796-rmjv56ia	13	24	this	this	DT
cord-300796-rmjv56ia	13	25	becomes	become	VBZ
cord-300796-rmjv56ia	13	26	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	13	27	early	early	RB
cord-300796-rmjv56ia	13	28	during	during	IN
cord-300796-rmjv56ia	13	29	synthesis	synthesis	NN
cord-300796-rmjv56ia	13	30	and	and	CC
cord-300796-rmjv56ia	13	31	translocation	translocation	NN
cord-300796-rmjv56ia	13	32	of	of	IN
cord-300796-rmjv56ia	13	33	the	the	DT
cord-300796-rmjv56ia	13	34	p62	p62	NN
cord-300796-rmjv56ia	13	35	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	13	36	.	.	.
cord-300796-rmjv56ia	14	1	As	as	IN
cord-300796-rmjv56ia	14	2	the	the	DT
cord-300796-rmjv56ia	14	3	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	14	4	of	of	IN
cord-300796-rmjv56ia	14	5	the	the	DT
cord-300796-rmjv56ia	14	6	signal	signal	NN
cord-300796-rmjv56ia	14	7	sequence	sequence	NN
cord-300796-rmjv56ia	14	8	most	most	RBS
cord-300796-rmjv56ia	14	9	likely	likely	RB
cord-300796-rmjv56ia	14	10	occurs	occur	VBZ
cord-300796-rmjv56ia	14	11	after	after	IN
cord-300796-rmjv56ia	14	12	its	-PRON-	PRP$
cord-300796-rmjv56ia	14	13	release	release	NN
cord-300796-rmjv56ia	14	14	from	from	IN
cord-300796-rmjv56ia	14	15	the	the	DT
cord-300796-rmjv56ia	14	16	ER	ER	NNP
cord-300796-rmjv56ia	14	17	membrane	membrane	NN
cord-300796-rmjv56ia	14	18	our	-PRON-	PRP$
cord-300796-rmjv56ia	14	19	results	result	NNS
cord-300796-rmjv56ia	14	20	suggest	suggest	VBP
cord-300796-rmjv56ia	14	21	that	that	IN
cord-300796-rmjv56ia	14	22	this	this	DT
cord-300796-rmjv56ia	14	23	region	region	NN
cord-300796-rmjv56ia	14	24	has	have	VBZ
cord-300796-rmjv56ia	14	25	no	no	DT
cord-300796-rmjv56ia	14	26	role	role	NN
cord-300796-rmjv56ia	14	27	in	in	IN
cord-300796-rmjv56ia	14	28	completing	complete	VBG
cord-300796-rmjv56ia	14	29	the	the	DT
cord-300796-rmjv56ia	14	30	transfer	transfer	NN
cord-300796-rmjv56ia	14	31	process	process	NN
cord-300796-rmjv56ia	14	32	.	.	.
cord-300796-rmjv56ia	15	1	IOSYNTHESIS	IOSYNTHESIS	NNP
cord-300796-rmjv56ia	15	2	of	of	IN
cord-300796-rmjv56ia	15	3	proteins	protein	NNS
cord-300796-rmjv56ia	15	4	at	at	IN
cord-300796-rmjv56ia	15	5	the	the	DT
cord-300796-rmjv56ia	15	6	ER	ER	NNP
cord-300796-rmjv56ia	15	7	can	can	MD
cord-300796-rmjv56ia	15	8	be	be	VB
cord-300796-rmjv56ia	15	9	subdivided	subdivide	VBN
cord-300796-rmjv56ia	15	10	into	into	IN
cord-300796-rmjv56ia	15	11	several	several	JJ
cord-300796-rmjv56ia	15	12	steps	step	NNS
cord-300796-rmjv56ia	15	13	.	.	.
cord-300796-rmjv56ia	16	1	These	these	DT
cord-300796-rmjv56ia	16	2	are	be	VBP
cord-300796-rmjv56ia	16	3	(	(	-LRB-
cord-300796-rmjv56ia	16	4	a	a	LS
cord-300796-rmjv56ia	16	5	)	)	-RRB-
cord-300796-rmjv56ia	16	6	targeting	targeting	NN
cord-300796-rmjv56ia	16	7	of	of	IN
cord-300796-rmjv56ia	16	8	translation	translation	NN
cord-300796-rmjv56ia	16	9	complexes	complex	NNS
cord-300796-rmjv56ia	16	10	to	to	IN
cord-300796-rmjv56ia	16	11	the	the	DT
cord-300796-rmjv56ia	16	12	ER	ER	NNP
cord-300796-rmjv56ia	16	13	membrane	membrane	NN
cord-300796-rmjv56ia	16	14	;	;	:
cord-300796-rmjv56ia	16	15	(	(	-LRB-
cord-300796-rmjv56ia	16	16	b	b	NN
cord-300796-rmjv56ia	16	17	)	)	-RRB-
cord-300796-rmjv56ia	16	18	synthesis	synthesis	NN
cord-300796-rmjv56ia	16	19	and	and	CC
cord-300796-rmjv56ia	16	20	transfer	transfer	NN
cord-300796-rmjv56ia	16	21	(	(	-LRB-
cord-300796-rmjv56ia	16	22	translocation	translocation	NN
cord-300796-rmjv56ia	16	23	)	)	-RRB-
cord-300796-rmjv56ia	16	24	of	of	IN
cord-300796-rmjv56ia	16	25	the	the	DT
cord-300796-rmjv56ia	16	26	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	16	27	chain	chain	NN
cord-300796-rmjv56ia	16	28	across	across	IN
cord-300796-rmjv56ia	16	29	the	the	DT
cord-300796-rmjv56ia	16	30	lipid	lipid	NN
cord-300796-rmjv56ia	16	31	bilayer	bilayer	NN
cord-300796-rmjv56ia	16	32	;	;	:
cord-300796-rmjv56ia	16	33	and	and	CC
cord-300796-rmjv56ia	16	34	(	(	-LRB-
cord-300796-rmjv56ia	16	35	c	c	NN
cord-300796-rmjv56ia	16	36	)	)	-RRB-
cord-300796-rmjv56ia	16	37	protein	protein	NN
cord-300796-rmjv56ia	16	38	maturation	maturation	NN
cord-300796-rmjv56ia	16	39	in	in	IN
cord-300796-rmjv56ia	16	40	the	the	DT
cord-300796-rmjv56ia	16	41	lumen	lumen	NN
cord-300796-rmjv56ia	16	42	of	of	IN
cord-300796-rmjv56ia	16	43	ER	ER	NNP
cord-300796-rmjv56ia	16	44	(	(	-LRB-
cord-300796-rmjv56ia	16	45	chain	chain	NN
cord-300796-rmjv56ia	16	46	folding	folding	NN
cord-300796-rmjv56ia	16	47	,	,	,
cord-300796-rmjv56ia	16	48	disulphide	disulphide	JJ
cord-300796-rmjv56ia	16	49	bridge	bridge	NN
cord-300796-rmjv56ia	16	50	formation	formation	NN
cord-300796-rmjv56ia	16	51	,	,	,
cord-300796-rmjv56ia	16	52	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	16	53	,	,	,
cord-300796-rmjv56ia	16	54	and	and	CC
cord-300796-rmjv56ia	16	55	oligomerization	oligomerization	NN
cord-300796-rmjv56ia	16	56	)	)	-RRB-
cord-300796-rmjv56ia	16	57	.	.	.
cord-300796-rmjv56ia	17	1	The	the	DT
cord-300796-rmjv56ia	17	2	mechanisms	mechanism	NNS
cord-300796-rmjv56ia	17	3	for	for	IN
cord-300796-rmjv56ia	17	4	these	these	DT
cord-300796-rmjv56ia	17	5	processes	process	NNS
cord-300796-rmjv56ia	17	6	have	have	VBP
cord-300796-rmjv56ia	17	7	been	be	VBN
cord-300796-rmjv56ia	17	8	studied	study	VBN
cord-300796-rmjv56ia	17	9	extensively	extensively	RB
cord-300796-rmjv56ia	17	10	during	during	IN
cord-300796-rmjv56ia	17	11	recent	recent	JJ
cord-300796-rmjv56ia	17	12	years	year	NNS
cord-300796-rmjv56ia	17	13	(	(	-LRB-
cord-300796-rmjv56ia	17	14	Kornfeld	Kornfeld	NNP
cord-300796-rmjv56ia	17	15	and	and	CC
cord-300796-rmjv56ia	17	16	Kornfeld	Kornfeld	NNP
cord-300796-rmjv56ia	17	17	,	,	,
cord-300796-rmjv56ia	17	18	1985	1985	CD
cord-300796-rmjv56ia	17	19	;	;	:
cord-300796-rmjv56ia	17	20	Wickner	Wickner	NNP
cord-300796-rmjv56ia	17	21	and	and	CC
cord-300796-rmjv56ia	17	22	Lodish	Lodish	NNP
cord-300796-rmjv56ia	17	23	,	,	,
cord-300796-rmjv56ia	17	24	1985	1985	CD
cord-300796-rmjv56ia	17	25	;	;	:
cord-300796-rmjv56ia	17	26	Rapoport	Rapoport	NNP
cord-300796-rmjv56ia	17	27	,	,	,
cord-300796-rmjv56ia	17	28	1986	1986	CD
cord-300796-rmjv56ia	17	29	;	;	:
cord-300796-rmjv56ia	17	30	Lodish	Lodish	NNP
cord-300796-rmjv56ia	17	31	,	,	,
cord-300796-rmjv56ia	17	32	1988	1988	CD
cord-300796-rmjv56ia	17	33	;	;	:
cord-300796-rmjv56ia	17	34	Rothman	Rothman	NNP
cord-300796-rmjv56ia	17	35	,	,	,
cord-300796-rmjv56ia	17	36	1989	1989	CD
cord-300796-rmjv56ia	17	37	)	)	-RRB-
cord-300796-rmjv56ia	17	38	.	.	.
cord-300796-rmjv56ia	18	1	A	a	DT
cord-300796-rmjv56ia	18	2	most	most	RBS
cord-300796-rmjv56ia	18	3	important	important	JJ
cord-300796-rmjv56ia	18	4	finding	finding	NN
cord-300796-rmjv56ia	18	5	has	have	VBZ
cord-300796-rmjv56ia	18	6	been	be	VBN
cord-300796-rmjv56ia	18	7	that	that	IN
cord-300796-rmjv56ia	18	8	all	all	DT
cord-300796-rmjv56ia	18	9	proteins	protein	NNS
cord-300796-rmjv56ia	18	10	made	make	VBN
cord-300796-rmjv56ia	18	11	at	at	IN
cord-300796-rmjv56ia	18	12	the	the	DT
cord-300796-rmjv56ia	18	13	ER	ER	NNP
cord-300796-rmjv56ia	18	14	carry	carry	VB
cord-300796-rmjv56ia	18	15	a	a	DT
cord-300796-rmjv56ia	18	16	signal	signal	NN
cord-300796-rmjv56ia	18	17	sequence	sequence	NN
cord-300796-rmjv56ia	18	18	(	(	-LRB-
cord-300796-rmjv56ia	18	19	also	also	RB
cord-300796-rmjv56ia	18	20	called	call	VBN
cord-300796-rmjv56ia	18	21	signal	signal	JJ
cord-300796-rmjv56ia	18	22	peptide	peptide	NN
cord-300796-rmjv56ia	18	23	)	)	-RRB-
cord-300796-rmjv56ia	18	24	,	,	,
cord-300796-rmjv56ia	18	25	a	a	DT
cord-300796-rmjv56ia	18	26	hydrophobic	hydrophobic	JJ
cord-300796-rmjv56ia	18	27	peptide	peptide	NN
cord-300796-rmjv56ia	18	28	which	which	WDT
cord-300796-rmjv56ia	18	29	is	be	VBZ
cord-300796-rmjv56ia	18	30	usually	usually	RB
cord-300796-rmjv56ia	18	31	located	locate	VBN
cord-300796-rmjv56ia	18	32	at	at	IN
cord-300796-rmjv56ia	18	33	the	the	DT
cord-300796-rmjv56ia	18	34	NH2-terminal	NH2-terminal	NNP
cord-300796-rmjv56ia	18	35	region	region	NN
cord-300796-rmjv56ia	18	36	of	of	IN
cord-300796-rmjv56ia	18	37	the	the	DT
cord-300796-rmjv56ia	18	38	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	18	39	chain	chain	NN
cord-300796-rmjv56ia	18	40	.	.	.
cord-300796-rmjv56ia	19	1	One	one	CD
cord-300796-rmjv56ia	19	2	function	function	NN
cord-300796-rmjv56ia	19	3	of	of	IN
cord-300796-rmjv56ia	19	4	the	the	DT
cord-300796-rmjv56ia	19	5	signal	signal	JJ
cord-300796-rmjv56ia	19	6	peptide	peptide	NN
cord-300796-rmjv56ia	19	7	is	be	VBZ
cord-300796-rmjv56ia	19	8	to	to	TO
cord-300796-rmjv56ia	19	9	achieve	achieve	VB
cord-300796-rmjv56ia	19	10	targeting	targeting	NN
cord-300796-rmjv56ia	19	11	of	of	IN
cord-300796-rmjv56ia	19	12	the	the	DT
cord-300796-rmjv56ia	19	13	polysome	polysome	NN
cord-300796-rmjv56ia	19	14	to	to	IN
cord-300796-rmjv56ia	19	15	the	the	DT
cord-300796-rmjv56ia	19	16	ER	ER	NNP
cord-300796-rmjv56ia	19	17	membrane	membrane	NN
cord-300796-rmjv56ia	19	18	(	(	-LRB-
cord-300796-rmjv56ia	19	19	Rapoport	Rapoport	NNP
cord-300796-rmjv56ia	19	20	,	,	,
cord-300796-rmjv56ia	19	21	1986	1986	CD
cord-300796-rmjv56ia	19	22	)	)	-RRB-
cord-300796-rmjv56ia	19	23	.	.	.
cord-300796-rmjv56ia	20	1	When	when	WRB
cord-300796-rmjv56ia	20	2	the	the	DT
cord-300796-rmjv56ia	20	3	signal	signal	NN
cord-300796-rmjv56ia	20	4	sequence	sequence	NN
cord-300796-rmjv56ia	20	5	emerges	emerge	VBZ
cord-300796-rmjv56ia	20	6	from	from	IN
cord-300796-rmjv56ia	20	7	the	the	DT
cord-300796-rmjv56ia	20	8	ribosome	ribosome	NN
cord-300796-rmjv56ia	20	9	it	-PRON-	PRP
cord-300796-rmjv56ia	20	10	binds	bind	VBZ
cord-300796-rmjv56ia	20	11	to	to	IN
cord-300796-rmjv56ia	20	12	the	the	DT
cord-300796-rmjv56ia	20	13	signal	signal	NN
cord-300796-rmjv56ia	20	14	recognition	recognition	NN
cord-300796-rmjv56ia	20	15	particle	particle	NN
cord-300796-rmjv56ia	20	16	,	,	,
cord-300796-rmjv56ia	20	17	which	which	WDT
cord-300796-rmjv56ia	20	18	mediates	mediate	VBZ
cord-300796-rmjv56ia	20	19	binding	binding	NN
cord-300796-rmjv56ia	20	20	of	of	IN
cord-300796-rmjv56ia	20	21	the	the	DT
cord-300796-rmjv56ia	20	22	polysome	polysome	NN
cord-300796-rmjv56ia	20	23	to	to	IN
cord-300796-rmjv56ia	20	24	the	the	DT
cord-300796-rmjv56ia	20	25	docking	docking	NN
cord-300796-rmjv56ia	20	26	protein	protein	NN
cord-300796-rmjv56ia	20	27	in	in	IN
cord-300796-rmjv56ia	20	28	the	the	DT
cord-300796-rmjv56ia	20	29	ER	ER	NNP
cord-300796-rmjv56ia	20	30	.	.	.
cord-300796-rmjv56ia	21	1	After	after	IN
cord-300796-rmjv56ia	21	2	this	this	DT
cord-300796-rmjv56ia	21	3	another	another	DT
cord-300796-rmjv56ia	21	4	function	function	NN
cord-300796-rmjv56ia	21	5	of	of	IN
cord-300796-rmjv56ia	21	6	the	the	DT
cord-300796-rmjv56ia	21	7	signal	signal	JJ
cord-300796-rmjv56ia	21	8	sequence	sequence	NN
cord-300796-rmjv56ia	21	9	is	be	VBZ
cord-300796-rmjv56ia	21	10	expressed	express	VBN
cord-300796-rmjv56ia	21	11	,	,	,
cord-300796-rmjv56ia	21	12	that	that	DT
cord-300796-rmjv56ia	21	13	is	be	VBZ
cord-300796-rmjv56ia	21	14	to	to	TO
cord-300796-rmjv56ia	21	15	interact	interact	VB
cord-300796-rmjv56ia	21	16	with	with	IN
cord-300796-rmjv56ia	21	17	some	some	DT
cord-300796-rmjv56ia	21	18	components	component	NNS
cord-300796-rmjv56ia	21	19	of	of	IN
cord-300796-rmjv56ia	21	20	the	the	DT
cord-300796-rmjv56ia	21	21	ER	ER	NNP
cord-300796-rmjv56ia	21	22	membrane	membrane	NN
cord-300796-rmjv56ia	21	23	and	and	CC
cord-300796-rmjv56ia	21	24	thereby	thereby	RB
cord-300796-rmjv56ia	21	25	initiate	initiate	VB
cord-300796-rmjv56ia	21	26	translocation	translocation	NN
cord-300796-rmjv56ia	21	27	of	of	IN
cord-300796-rmjv56ia	21	28	the	the	DT
cord-300796-rmjv56ia	21	29	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	21	30	chain	chain	NN
cord-300796-rmjv56ia	21	31	into	into	IN
cord-300796-rmjv56ia	21	32	the	the	DT
cord-300796-rmjv56ia	21	33	lumen	lumen	NN
cord-300796-rmjv56ia	21	34	of	of	IN
cord-300796-rmjv56ia	21	35	the	the	DT
cord-300796-rmjv56ia	21	36	ER	ER	NNP
cord-300796-rmjv56ia	21	37	(	(	-LRB-
cord-300796-rmjv56ia	21	38	Gilmore	Gilmore	NNP
cord-300796-rmjv56ia	21	39	and	and	CC
cord-300796-rmjv56ia	21	40	Blobel	Blobel	NNP
cord-300796-rmjv56ia	21	41	,	,	,
cord-300796-rmjv56ia	21	42	1985	1985	CD
cord-300796-rmjv56ia	21	43	;	;	:
cord-300796-rmjv56ia	21	44	Robinson	Robinson	NNP
cord-300796-rmjv56ia	21	45	et	et	NNP
cord-300796-rmjv56ia	21	46	al	al	NNP
cord-300796-rmjv56ia	21	47	.	.	NNP
cord-300796-rmjv56ia	21	48	,	,	,
cord-300796-rmjv56ia	21	49	1987	1987	CD
cord-300796-rmjv56ia	21	50	;	;	:
cord-300796-rmjv56ia	21	51	Wiedmann	Wiedmann	NNP
cord-300796-rmjv56ia	21	52	et	et	NNP
cord-300796-rmjv56ia	21	53	al	al	NNP
cord-300796-rmjv56ia	21	54	.	.	NNP
cord-300796-rmjv56ia	21	55	,	,	,
cord-300796-rmjv56ia	21	56	1987	1987	CD
cord-300796-rmjv56ia	21	57	)	)	-RRB-
cord-300796-rmjv56ia	21	58	.	.	.
cord-300796-rmjv56ia	22	1	Further	further	JJ
cord-300796-rmjv56ia	22	2	synthesis	synthesis	NN
cord-300796-rmjv56ia	22	3	of	of	IN
cord-300796-rmjv56ia	22	4	the	the	DT
cord-300796-rmjv56ia	22	5	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	22	6	then	then	RB
cord-300796-rmjv56ia	22	7	continues	continue	VBZ
cord-300796-rmjv56ia	22	8	with	with	IN
cord-300796-rmjv56ia	22	9	concomitant	concomitant	JJ
cord-300796-rmjv56ia	22	10	chain	chain	NN
cord-300796-rmjv56ia	22	11	translocation	translocation	NN
cord-300796-rmjv56ia	22	12	.	.	.
cord-300796-rmjv56ia	23	1	An	an	DT
cord-300796-rmjv56ia	23	2	important	important	JJ
cord-300796-rmjv56ia	23	3	but	but	CC
cord-300796-rmjv56ia	23	4	as	as	IN
cord-300796-rmjv56ia	23	5	yet	yet	RB
cord-300796-rmjv56ia	23	6	unresolved	unresolved	JJ
cord-300796-rmjv56ia	23	7	question	question	NN
cord-300796-rmjv56ia	23	8	is	be	VBZ
cord-300796-rmjv56ia	23	9	whether	whether	IN
cord-300796-rmjv56ia	23	10	the	the	DT
cord-300796-rmjv56ia	23	11	signal	signal	NN
cord-300796-rmjv56ia	23	12	sequence	sequence	NN
cord-300796-rmjv56ia	23	13	has	have	VBZ
cord-300796-rmjv56ia	23	14	any	any	DT
cord-300796-rmjv56ia	23	15	role	role	NN
cord-300796-rmjv56ia	23	16	in	in	IN
cord-300796-rmjv56ia	23	17	the	the	DT
cord-300796-rmjv56ia	23	18	translocation	translocation	NN
cord-300796-rmjv56ia	23	19	process	process	NN
cord-300796-rmjv56ia	23	20	per	per	FW
cord-300796-rmjv56ia	23	21	se	se	FW
cord-300796-rmjv56ia	23	22	or	or	CC
cord-300796-rmjv56ia	23	23	whether	whether	IN
cord-300796-rmjv56ia	23	24	its	-PRON-	PRP$
cord-300796-rmjv56ia	23	25	functions	function	NNS
cord-300796-rmjv56ia	23	26	are	be	VBP
cord-300796-rmjv56ia	23	27	limited	limit	VBN
cord-300796-rmjv56ia	23	28	to	to	IN
cord-300796-rmjv56ia	23	29	the	the	DT
cord-300796-rmjv56ia	23	30	targeting	targeting	NN
cord-300796-rmjv56ia	23	31	and	and	CC
cord-300796-rmjv56ia	23	32	translocation	translocation	NN
cord-300796-rmjv56ia	23	33	-	-	HYPH
cord-300796-rmjv56ia	23	34	initiation	initiation	NN
cord-300796-rmjv56ia	23	35	steps	step	NNS
cord-300796-rmjv56ia	23	36	.	.	.
cord-300796-rmjv56ia	24	1	For	for	IN
cord-300796-rmjv56ia	24	2	instance	instance	NN
cord-300796-rmjv56ia	24	3	,	,	,
cord-300796-rmjv56ia	24	4	Singer	singer	NN
cord-300796-rmjv56ia	24	5	and	and	CC
cord-300796-rmjv56ia	24	6	co	co	NNS
cord-300796-rmjv56ia	24	7	-	-	NNS
cord-300796-rmjv56ia	24	8	workers	worker	NNS
cord-300796-rmjv56ia	24	9	Danny	Danny	NNP
cord-300796-rmjv56ia	24	10	Huylebroeck	Huylebroeck	NNP
cord-300796-rmjv56ia	24	11	's	's	POS
cord-300796-rmjv56ia	24	12	present	present	JJ
cord-300796-rmjv56ia	24	13	address	address	NN
cord-300796-rmjv56ia	24	14	is	be	VBZ
cord-300796-rmjv56ia	24	15	Innogenetics	Innogenetics	NNP
cord-300796-rmjv56ia	24	16	,	,	,
cord-300796-rmjv56ia	24	17	Industriepark	Industriepark	NNP
cord-300796-rmjv56ia	24	18	7	7	CD
cord-300796-rmjv56ia	24	19	,	,	,
cord-300796-rmjv56ia	24	20	Box	Box	NNP
cord-300796-rmjv56ia	24	21	4	4	CD
cord-300796-rmjv56ia	24	22	,	,	,
cord-300796-rmjv56ia	24	23	B-9710	B-9710	NNS
cord-300796-rmjv56ia	24	24	,	,	,
cord-300796-rmjv56ia	24	25	Ghent	Ghent	NNP
cord-300796-rmjv56ia	24	26	,	,	,
cord-300796-rmjv56ia	24	27	Belgium	Belgium	NNP
cord-300796-rmjv56ia	24	28	.	.	.
cord-300796-rmjv56ia	25	1	(	(	-LRB-
cord-300796-rmjv56ia	25	2	1987a	1987a	CD
cord-300796-rmjv56ia	25	3	)	)	-RRB-
cord-300796-rmjv56ia	25	4	have	have	VBP
cord-300796-rmjv56ia	25	5	suggested	suggest	VBN
cord-300796-rmjv56ia	25	6	a	a	DT
cord-300796-rmjv56ia	25	7	translocator	translocator	NN
cord-300796-rmjv56ia	25	8	protein	protein	NN
cord-300796-rmjv56ia	25	9	model	model	NN
cord-300796-rmjv56ia	25	10	in	in	IN
cord-300796-rmjv56ia	25	11	which	which	WDT
cord-300796-rmjv56ia	25	12	the	the	DT
cord-300796-rmjv56ia	25	13	signal	signal	NN
cord-300796-rmjv56ia	25	14	sequence	sequence	NN
cord-300796-rmjv56ia	25	15	helps	help	VBZ
cord-300796-rmjv56ia	25	16	to	to	TO
cord-300796-rmjv56ia	25	17	keep	keep	VB
cord-300796-rmjv56ia	25	18	the	the	DT
cord-300796-rmjv56ia	25	19	machinery	machinery	NN
cord-300796-rmjv56ia	25	20	open	open	JJ
cord-300796-rmjv56ia	25	21	for	for	IN
cord-300796-rmjv56ia	25	22	chain	chain	NN
cord-300796-rmjv56ia	25	23	transfer	transfer	NN
cord-300796-rmjv56ia	25	24	.	.	.
cord-300796-rmjv56ia	26	1	It	-PRON-	PRP
cord-300796-rmjv56ia	26	2	is	be	VBZ
cord-300796-rmjv56ia	26	3	specifically	specifically	RB
cord-300796-rmjv56ia	26	4	this	this	DT
cord-300796-rmjv56ia	26	5	last	last	JJ
cord-300796-rmjv56ia	26	6	question	question	NN
cord-300796-rmjv56ia	26	7	we	-PRON-	PRP
cord-300796-rmjv56ia	26	8	have	have	VBP
cord-300796-rmjv56ia	26	9	addressed	address	VBN
cord-300796-rmjv56ia	26	10	in	in	IN
cord-300796-rmjv56ia	26	11	the	the	DT
cord-300796-rmjv56ia	26	12	present	present	JJ
cord-300796-rmjv56ia	26	13	work	work	NN
cord-300796-rmjv56ia	26	14	.	.	.
cord-300796-rmjv56ia	27	1	We	-PRON-	PRP
cord-300796-rmjv56ia	27	2	describe	describe	VBP
cord-300796-rmjv56ia	27	3	the	the	DT
cord-300796-rmjv56ia	27	4	characteristics	characteristic	NNS
cord-300796-rmjv56ia	27	5	and	and	CC
cord-300796-rmjv56ia	27	6	behavior	behavior	NN
cord-300796-rmjv56ia	27	7	of	of	IN
cord-300796-rmjv56ia	27	8	the	the	DT
cord-300796-rmjv56ia	27	9	uncleaved	uncleaved	JJ
cord-300796-rmjv56ia	27	10	signal	signal	NN
cord-300796-rmjv56ia	27	11	sequence	sequence	NN
cord-300796-rmjv56ia	27	12	of	of	IN
cord-300796-rmjv56ia	27	13	the	the	DT
cord-300796-rmjv56ia	27	14	p62	p62	NN
cord-300796-rmjv56ia	27	15	protein	protein	NN
cord-300796-rmjv56ia	27	16	of	of	IN
cord-300796-rmjv56ia	27	17	Semliki	Semliki	NNP
cord-300796-rmjv56ia	27	18	Forest	Forest	NNP
cord-300796-rmjv56ia	27	19	virus	virus	NN
cord-300796-rmjv56ia	27	20	(	(	-LRB-
cord-300796-rmjv56ia	27	21	SFV	SFV	NNP
cord-300796-rmjv56ia	27	22	)	)	-RRB-
cord-300796-rmjv56ia	27	23	l	l	NN
cord-300796-rmjv56ia	27	24	upon	upon	IN
cord-300796-rmjv56ia	27	25	translocation	translocation	NN
cord-300796-rmjv56ia	27	26	across	across	IN
cord-300796-rmjv56ia	27	27	the	the	DT
cord-300796-rmjv56ia	27	28	ER	ER	NNP
cord-300796-rmjv56ia	27	29	membrane	membrane	NN
cord-300796-rmjv56ia	27	30	in	in	IN
cord-300796-rmjv56ia	27	31	vitro	vitro	FW
cord-300796-rmjv56ia	27	32	.	.	.
cord-300796-rmjv56ia	28	1	The	the	DT
cord-300796-rmjv56ia	28	2	p62	p62	NN
cord-300796-rmjv56ia	28	3	protein	protein	NN
cord-300796-rmjv56ia	28	4	is	be	VBZ
cord-300796-rmjv56ia	28	5	one	one	CD
cord-300796-rmjv56ia	28	6	subunit	subunit	NN
cord-300796-rmjv56ia	28	7	of	of	IN
cord-300796-rmjv56ia	28	8	the	the	DT
cord-300796-rmjv56ia	28	9	heterodimeric	heterodimeric	NNP
cord-300796-rmjv56ia	28	10	spike	spike	NN
cord-300796-rmjv56ia	28	11	protein	protein	NN
cord-300796-rmjv56ia	28	12	of	of	IN
cord-300796-rmjv56ia	28	13	the	the	DT
cord-300796-rmjv56ia	28	14	SFV	SFV	NNP
cord-300796-rmjv56ia	28	15	membrane	membrane	NN
cord-300796-rmjv56ia	28	16	(	(	-LRB-
cord-300796-rmjv56ia	28	17	reviewed	review	VBN
cord-300796-rmjv56ia	28	18	in	in	IN
cord-300796-rmjv56ia	28	19	Garoff	Garoff	NNP
cord-300796-rmjv56ia	28	20	et	et	NNP
cord-300796-rmjv56ia	28	21	al	al	NNP
cord-300796-rmjv56ia	28	22	.	.	NNP
cord-300796-rmjv56ia	28	23	,	,	,
cord-300796-rmjv56ia	28	24	1982	1982	CD
cord-300796-rmjv56ia	28	25	)	)	-RRB-
cord-300796-rmjv56ia	28	26	.	.	.
cord-300796-rmjv56ia	29	1	It	-PRON-	PRP
cord-300796-rmjv56ia	29	2	is	be	VBZ
cord-300796-rmjv56ia	29	3	made	make	VBN
cord-300796-rmjv56ia	29	4	as	as	IN
cord-300796-rmjv56ia	29	5	a	a	DT
cord-300796-rmjv56ia	29	6	precursor	precursor	NN
cord-300796-rmjv56ia	29	7	protein	protein	NN
cord-300796-rmjv56ia	29	8	together	together	RB
cord-300796-rmjv56ia	29	9	with	with	IN
cord-300796-rmjv56ia	29	10	the	the	DT
cord-300796-rmjv56ia	29	11	other	other	JJ
cord-300796-rmjv56ia	29	12	structural	structural	JJ
cord-300796-rmjv56ia	29	13	proteins	protein	NNS
cord-300796-rmjv56ia	29	14	of	of	IN
cord-300796-rmjv56ia	29	15	SFV	sfv	NN
cord-300796-rmjv56ia	29	16	,	,	,
cord-300796-rmjv56ia	29	17	i.e.	i.e.	FW
cord-300796-rmjv56ia	29	18	,	,	,
cord-300796-rmjv56ia	29	19	the	the	DT
cord-300796-rmjv56ia	29	20	nucleocapsid	nucleocapsid	NNP
cord-300796-rmjv56ia	29	21	protein	protein	NN
cord-300796-rmjv56ia	29	22	,	,	,
cord-300796-rmjv56ia	29	23	C	C	NNP
cord-300796-rmjv56ia	29	24	,	,	,
cord-300796-rmjv56ia	29	25	and	and	CC
cord-300796-rmjv56ia	29	26	the	the	DT
cord-300796-rmjv56ia	29	27	other	other	JJ
cord-300796-rmjv56ia	29	28	spike	spike	NN
cord-300796-rmjv56ia	29	29	subunit	subunit	NN
cord-300796-rmjv56ia	29	30	,	,	,
cord-300796-rmjv56ia	29	31	El	El	NNP
cord-300796-rmjv56ia	29	32	.	.	.
cord-300796-rmjv56ia	30	1	The	the	DT
cord-300796-rmjv56ia	30	2	three	three	CD
cord-300796-rmjv56ia	30	3	proteins	protein	NNS
cord-300796-rmjv56ia	30	4	are	be	VBP
cord-300796-rmjv56ia	30	5	synthesized	synthesize	VBN
cord-300796-rmjv56ia	30	6	from	from	IN
cord-300796-rmjv56ia	30	7	a	a	DT
cord-300796-rmjv56ia	30	8	4.1-kb	4.1-kb	CD
cord-300796-rmjv56ia	30	9	long	long	JJ
cord-300796-rmjv56ia	30	10	mRNA	mrna	NN
cord-300796-rmjv56ia	30	11	in	in	IN
cord-300796-rmjv56ia	30	12	the	the	DT
cord-300796-rmjv56ia	30	13	order	order	NN
cord-300796-rmjv56ia	30	14	C	c	NN
cord-300796-rmjv56ia	30	15	,	,	,
cord-300796-rmjv56ia	30	16	p62	p62	NN
cord-300796-rmjv56ia	30	17	,	,	,
cord-300796-rmjv56ia	30	18	and	and	CC
cord-300796-rmjv56ia	30	19	El	El	NNP
cord-300796-rmjv56ia	30	20	,	,	,
cord-300796-rmjv56ia	30	21	and	and	CC
cord-300796-rmjv56ia	30	22	separated	separate	VBN
cord-300796-rmjv56ia	30	23	by	by	IN
cord-300796-rmjv56ia	30	24	cleavage	cleavage	NN
cord-300796-rmjv56ia	30	25	of	of	IN
cord-300796-rmjv56ia	30	26	the	the	DT
cord-300796-rmjv56ia	30	27	growing	grow	VBG
cord-300796-rmjv56ia	30	28	precursor	precursor	NN
cord-300796-rmjv56ia	30	29	chain	chain	NN
cord-300796-rmjv56ia	30	30	.	.	.
cord-300796-rmjv56ia	31	1	During	during	IN
cord-300796-rmjv56ia	31	2	synthesis	synthesis	NN
cord-300796-rmjv56ia	31	3	of	of	IN
cord-300796-rmjv56ia	31	4	the	the	DT
cord-300796-rmjv56ia	31	5	p62	p62	NN
cord-300796-rmjv56ia	31	6	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	31	7	at	at	IN
cord-300796-rmjv56ia	31	8	the	the	DT
cord-300796-rmjv56ia	31	9	ER	ER	NNP
cord-300796-rmjv56ia	31	10	all	all	RB
cord-300796-rmjv56ia	31	11	but	but	CC
cord-300796-rmjv56ia	31	12	a	a	DT
cord-300796-rmjv56ia	31	13	31	31	CD
cord-300796-rmjv56ia	31	14	residue	residue	NN
cord-300796-rmjv56ia	31	15	COOH	COOH	NNP
cord-300796-rmjv56ia	31	16	-	-	HYPH
cord-300796-rmjv56ia	31	17	terminal	terminal	NN
cord-300796-rmjv56ia	31	18	portion	portion	NN
cord-300796-rmjv56ia	31	19	and	and	CC
cord-300796-rmjv56ia	31	20	the	the	DT
cord-300796-rmjv56ia	31	21	membrane	membrane	NN
cord-300796-rmjv56ia	31	22	anchor	anchor	NN
cord-300796-rmjv56ia	31	23	is	be	VBZ
cord-300796-rmjv56ia	31	24	translocated	translocate	VBN
cord-300796-rmjv56ia	31	25	across	across	IN
cord-300796-rmjv56ia	31	26	the	the	DT
cord-300796-rmjv56ia	31	27	membrane	membrane	NN
cord-300796-rmjv56ia	31	28	.	.	.
cord-300796-rmjv56ia	32	1	The	the	DT
cord-300796-rmjv56ia	32	2	p62	p62	NN
cord-300796-rmjv56ia	32	3	signal	signal	NN
cord-300796-rmjv56ia	32	4	sequence	sequence	NN
cord-300796-rmjv56ia	32	5	has	have	VBZ
cord-300796-rmjv56ia	32	6	so	so	RB
cord-300796-rmjv56ia	32	7	far	far	RB
cord-300796-rmjv56ia	32	8	been	be	VBN
cord-300796-rmjv56ia	32	9	only	only	RB
cord-300796-rmjv56ia	32	10	roughly	roughly	RB
cord-300796-rmjv56ia	32	11	localized	localize	VBN
cord-300796-rmjv56ia	32	12	to	to	IN
cord-300796-rmjv56ia	32	13	the	the	DT
cord-300796-rmjv56ia	32	14	NH2-terminal	nh2-terminal	JJ
cord-300796-rmjv56ia	32	15	third	third	NN
cord-300796-rmjv56ia	32	16	of	of	IN
cord-300796-rmjv56ia	32	17	the	the	DT
cord-300796-rmjv56ia	32	18	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	32	19	chain	chain	NN
cord-300796-rmjv56ia	33	1	(	(	-LRB-
cord-300796-rmjv56ia	33	2	Garoffet	Garoffet	NNP
cord-300796-rmjv56ia	33	3	al	al	NNP
cord-300796-rmjv56ia	33	4	.	.	.
cord-300796-rmjv56ia	34	1	,	,	,
cord-300796-rmjv56ia	34	2	1978	1978	CD
cord-300796-rmjv56ia	34	3	;	;	:
cord-300796-rmjv56ia	34	4	Bonatti	Bonatti	NNP
cord-300796-rmjv56ia	34	5	et	et	NNP
cord-300796-rmjv56ia	34	6	al	al	NNP
cord-300796-rmjv56ia	34	7	.	.	NNP
cord-300796-rmjv56ia	34	8	,	,	,
cord-300796-rmjv56ia	34	9	1984	1984	CD
cord-300796-rmjv56ia	34	10	)	)	-RRB-
cord-300796-rmjv56ia	34	11	.	.	.
cord-300796-rmjv56ia	35	1	We	-PRON-	PRP
cord-300796-rmjv56ia	35	2	show	show	VBP
cord-300796-rmjv56ia	35	3	here	here	RB
cord-300796-rmjv56ia	35	4	that	that	IN
cord-300796-rmjv56ia	35	5	the	the	DT
cord-300796-rmjv56ia	35	6	signal	signal	JJ
cord-300796-rmjv56ia	35	7	sequence	sequence	NN
cord-300796-rmjv56ia	35	8	of	of	IN
cord-300796-rmjv56ia	35	9	p62	p62	NN
cord-300796-rmjv56ia	35	10	consists	consist	VBZ
cord-300796-rmjv56ia	35	11	of	of	IN
cord-300796-rmjv56ia	35	12	a	a	DT
cord-300796-rmjv56ia	35	13	16	16	CD
cord-300796-rmjv56ia	35	14	residue	residue	NN
cord-300796-rmjv56ia	35	15	peptide	peptide	NN
cord-300796-rmjv56ia	35	16	at	at	IN
cord-300796-rmjv56ia	35	17	its	-PRON-	PRP$
cord-300796-rmjv56ia	35	18	NH2-terminal	nh2-terminal	JJ
cord-300796-rmjv56ia	35	19	region	region	NN
cord-300796-rmjv56ia	35	20	.	.	.
cord-300796-rmjv56ia	36	1	This	this	DT
cord-300796-rmjv56ia	36	2	region	region	NN
cord-300796-rmjv56ia	36	3	includes	include	VBZ
cord-300796-rmjv56ia	36	4	one	one	CD
cord-300796-rmjv56ia	36	5	out	out	IN
cord-300796-rmjv56ia	36	6	of	of	IN
cord-300796-rmjv56ia	36	7	four	four	CD
cord-300796-rmjv56ia	36	8	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	36	9	sites	site	NNS
cord-300796-rmjv56ia	36	10	(	(	-LRB-
cord-300796-rmjv56ia	36	11	Asn~3	Asn~3	NNP
cord-300796-rmjv56ia	36	12	)	)	-RRB-
cord-300796-rmjv56ia	36	13	for	for	IN
cord-300796-rmjv56ia	36	14	N	n	CD
cord-300796-rmjv56ia	36	15	-	-	HYPH
cord-300796-rmjv56ia	36	16	linked	link	VBN
cord-300796-rmjv56ia	36	17	oligosaccharide	oligosaccharide	NN
cord-300796-rmjv56ia	36	18	on	on	IN
cord-300796-rmjv56ia	36	19	the	the	DT
cord-300796-rmjv56ia	36	20	p62	p62	NN
cord-300796-rmjv56ia	36	21	chain	chain	NN
cord-300796-rmjv56ia	36	22	.	.	.
cord-300796-rmjv56ia	37	1	We	-PRON-	PRP
cord-300796-rmjv56ia	37	2	also	also	RB
cord-300796-rmjv56ia	37	3	demonstrate	demonstrate	VBP
cord-300796-rmjv56ia	37	4	that	that	IN
cord-300796-rmjv56ia	37	5	the	the	DT
cord-300796-rmjv56ia	37	6	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	37	7	of	of	IN
cord-300796-rmjv56ia	37	8	the	the	DT
cord-300796-rmjv56ia	37	9	p62	p62	NN
cord-300796-rmjv56ia	37	10	signal	signal	NN
cord-300796-rmjv56ia	37	11	sequence	sequence	NN
cord-300796-rmjv56ia	37	12	occurs	occur	VBZ
cord-300796-rmjv56ia	37	13	early	early	RB
cord-300796-rmjv56ia	37	14	during	during	IN
cord-300796-rmjv56ia	37	15	chain	chain	NN
cord-300796-rmjv56ia	37	16	translocation	translocation	NN
cord-300796-rmjv56ia	37	17	.	.	.
cord-300796-rmjv56ia	38	1	As	as	IN
cord-300796-rmjv56ia	38	2	this	this	DT
cord-300796-rmjv56ia	38	3	modification	modification	NN
cord-300796-rmjv56ia	38	4	of	of	IN
cord-300796-rmjv56ia	38	5	the	the	DT
cord-300796-rmjv56ia	38	6	signal	signal	NN
cord-300796-rmjv56ia	38	7	region	region	NN
cord-300796-rmjv56ia	38	8	most	most	RBS
cord-300796-rmjv56ia	38	9	likely	likely	RB
cord-300796-rmjv56ia	38	10	correlates	correlate	VBZ
cord-300796-rmjv56ia	38	11	with	with	IN
cord-300796-rmjv56ia	38	12	its	-PRON-	PRP$
cord-300796-rmjv56ia	38	13	release	release	NN
cord-300796-rmjv56ia	38	14	into	into	IN
cord-300796-rmjv56ia	38	15	the	the	DT
cord-300796-rmjv56ia	38	16	lumen	lumen	NN
cord-300796-rmjv56ia	38	17	of	of	IN
cord-300796-rmjv56ia	38	18	ER	ER	NNP
cord-300796-rmjv56ia	38	19	it	-PRON-	PRP
cord-300796-rmjv56ia	38	20	follows	follow	VBZ
cord-300796-rmjv56ia	38	21	that	that	IN
cord-300796-rmjv56ia	38	22	the	the	DT
cord-300796-rmjv56ia	38	23	signal	signal	JJ
cord-300796-rmjv56ia	38	24	sequence	sequence	NN
cord-300796-rmjv56ia	38	25	of	of	IN
cord-300796-rmjv56ia	38	26	p62	p62	NN
cord-300796-rmjv56ia	38	27	is	be	VBZ
cord-300796-rmjv56ia	38	28	probably	probably	RB
cord-300796-rmjv56ia	38	29	only	only	RB
cord-300796-rmjv56ia	38	30	needed	need	VBN
cord-300796-rmjv56ia	38	31	for	for	IN
cord-300796-rmjv56ia	38	32	an	an	DT
cord-300796-rmjv56ia	38	33	initial	initial	JJ
cord-300796-rmjv56ia	38	34	step	step	NN
cord-300796-rmjv56ia	38	35	in	in	IN
cord-300796-rmjv56ia	38	36	chain	chain	NN
cord-300796-rmjv56ia	38	37	translocation	translocation	NN
cord-300796-rmjv56ia	38	38	and	and	CC
cord-300796-rmjv56ia	38	39	not	not	RB
cord-300796-rmjv56ia	38	40	to	to	IN
cord-300796-rmjv56ia	38	41	Small	small	JJ
cord-300796-rmjv56ia	38	42	scale	scale	NN
cord-300796-rmjv56ia	38	43	plasrnid	plasrnid	NN
cord-300796-rmjv56ia	38	44	DNA	dna	NN
cord-300796-rmjv56ia	38	45	preparations	preparation	NNS
cord-300796-rmjv56ia	38	46	were	be	VBD
cord-300796-rmjv56ia	38	47	done	do	VBN
cord-300796-rmjv56ia	38	48	using	use	VBG
cord-300796-rmjv56ia	38	49	the	the	DT
cord-300796-rmjv56ia	38	50	alkali	alkali	JJ
cord-300796-rmjv56ia	38	51	-	-	HYPH
cord-300796-rmjv56ia	38	52	SDS	sds	NN
cord-300796-rmjv56ia	38	53	method	method	NN
cord-300796-rmjv56ia	38	54	essentially	essentially	RB
cord-300796-rmjv56ia	38	55	as	as	IN
cord-300796-rmjv56ia	38	56	described	describe	VBN
cord-300796-rmjv56ia	38	57	by	by	IN
cord-300796-rmjv56ia	38	58	Birnboim	Birnboim	NNP
cord-300796-rmjv56ia	38	59	and	and	CC
cord-300796-rmjv56ia	38	60	Dnly	Dnly	NNP
cord-300796-rmjv56ia	38	61	(	(	-LRB-
cord-300796-rmjv56ia	38	62	1979	1979	CD
cord-300796-rmjv56ia	38	63	)	)	-RRB-
cord-300796-rmjv56ia	38	64	.	.	.
cord-300796-rmjv56ia	39	1	Large	large	JJ
cord-300796-rmjv56ia	39	2	quantities	quantity	NNS
cord-300796-rmjv56ia	39	3	of	of	IN
cord-300796-rmjv56ia	39	4	plasmids	plasmid	NNS
cord-300796-rmjv56ia	39	5	to	to	TO
cord-300796-rmjv56ia	39	6	be	be	VB
cord-300796-rmjv56ia	39	7	used	use	VBN
cord-300796-rmjv56ia	39	8	for	for	IN
cord-300796-rmjv56ia	39	9	in	in	FW
cord-300796-rmjv56ia	39	10	vitro	vitro	FW
cord-300796-rmjv56ia	39	11	transcription	transcription	NN
cord-300796-rmjv56ia	39	12	were	be	VBD
cord-300796-rmjv56ia	39	13	prepared	prepare	VBN
cord-300796-rmjv56ia	39	14	by	by	IN
cord-300796-rmjv56ia	39	15	lysozyme	lysozyme	NNS
cord-300796-rmjv56ia	39	16	-	-	HYPH
cord-300796-rmjv56ia	39	17	Triton	Triton	NNP
cord-300796-rmjv56ia	39	18	lysis	lysis	NN
cord-300796-rmjv56ia	39	19	of	of	IN
cord-300796-rmjv56ia	39	20	the	the	DT
cord-300796-rmjv56ia	39	21	bacteria	bacteria	NNS
cord-300796-rmjv56ia	39	22	,	,	,
cord-300796-rmjv56ia	39	23	followed	follow	VBN
cord-300796-rmjv56ia	39	24	by	by	IN
cord-300796-rmjv56ia	39	25	CsC1-EtBr	CsC1-EtBr	NNP
cord-300796-rmjv56ia	39	26	banding	banding	NN
cord-300796-rmjv56ia	39	27	(	(	-LRB-
cord-300796-rmjv56ia	39	28	Kahn	Kahn	NNP
cord-300796-rmjv56ia	39	29	et	et	NNP
cord-300796-rmjv56ia	39	30	al	al	NNP
cord-300796-rmjv56ia	39	31	.	.	NNP
cord-300796-rmjv56ia	39	32	,	,	,
cord-300796-rmjv56ia	39	33	1979	1979	CD
cord-300796-rmjv56ia	39	34	)	)	-RRB-
cord-300796-rmjv56ia	39	35	.	.	.
cord-300796-rmjv56ia	40	1	EtBr	EtBr	NNP
cord-300796-rmjv56ia	40	2	was	be	VBD
cord-300796-rmjv56ia	40	3	removed	remove	VBN
cord-300796-rmjv56ia	40	4	by	by	IN
cord-300796-rmjv56ia	40	5	several	several	JJ
cord-300796-rmjv56ia	40	6	extractions	extraction	NNS
cord-300796-rmjv56ia	40	7	with	with	IN
cord-300796-rmjv56ia	40	8	isopropanol	isopropanol	NN
cord-300796-rmjv56ia	40	9	and	and	CC
cord-300796-rmjv56ia	40	10	,	,	,
cord-300796-rmjv56ia	40	11	after	after	IN
cord-300796-rmjv56ia	40	12	fivefold	fivefold	JJ
cord-300796-rmjv56ia	40	13	dilution	dilution	NN
cord-300796-rmjv56ia	40	14	,	,	,
cord-300796-rmjv56ia	40	15	the	the	DT
cord-300796-rmjv56ia	40	16	DNA	dna	NN
cord-300796-rmjv56ia	40	17	was	be	VBD
cord-300796-rmjv56ia	40	18	precipitated	precipitate	VBN
cord-300796-rmjv56ia	40	19	twice	twice	RB
cord-300796-rmjv56ia	40	20	with	with	IN
cord-300796-rmjv56ia	40	21	ethanol	ethanol	NN
cord-300796-rmjv56ia	40	22	and	and	CC
cord-300796-rmjv56ia	40	23	further	further	RB
cord-300796-rmjv56ia	40	24	purified	purify	VBN
cord-300796-rmjv56ia	40	25	over	over	IN
cord-300796-rmjv56ia	40	26	a	a	DT
cord-300796-rmjv56ia	40	27	Biorad	Biorad	NNP
cord-300796-rmjv56ia	40	28	A-50	A-50	NNP
cord-300796-rmjv56ia	40	29	m	m	CD
cord-300796-rmjv56ia	40	30	column	column	NN
cord-300796-rmjv56ia	40	31	.	.	.
cord-300796-rmjv56ia	41	1	Restriction	restriction	NN
cord-300796-rmjv56ia	41	2	endonucleases	endonuclease	NNS
cord-300796-rmjv56ia	41	3	and	and	CC
cord-300796-rmjv56ia	41	4	DNA	dna	NN
cord-300796-rmjv56ia	41	5	-	-	HYPH
cord-300796-rmjv56ia	41	6	modifying	modify	VBG
cord-300796-rmjv56ia	41	7	enzymes	enzyme	NNS
cord-300796-rmjv56ia	41	8	were	be	VBD
cord-300796-rmjv56ia	41	9	used	use	VBN
cord-300796-rmjv56ia	41	10	according	accord	VBG
cord-300796-rmjv56ia	41	11	to	to	IN
cord-300796-rmjv56ia	41	12	the	the	DT
cord-300796-rmjv56ia	41	13	suppliers	supplier	NNS
cord-300796-rmjv56ia	41	14	instructions	instruction	NNS
cord-300796-rmjv56ia	41	15	.	.	.
cord-300796-rmjv56ia	42	1	Removal	removal	NN
cord-300796-rmjv56ia	42	2	of	of	IN
cord-300796-rmjv56ia	42	3	the	the	DT
cord-300796-rmjv56ia	42	4	3	3	CD
cord-300796-rmjv56ia	42	5	'	'	``
cord-300796-rmjv56ia	42	6	sticky	sticky	JJ
cord-300796-rmjv56ia	42	7	end	end	NN
cord-300796-rmjv56ia	42	8	from	from	IN
cord-300796-rmjv56ia	42	9	the	the	DT
cord-300796-rmjv56ia	42	10	Sac	Sac	NNP
cord-300796-rmjv56ia	42	11	I	-PRON-	PRP
cord-300796-rmjv56ia	42	12	site	site	VBP
cord-300796-rmjv56ia	42	13	in	in	IN
cord-300796-rmjv56ia	42	14	pGEM2-alphaG	pGEM2-alphaG	NNS
cord-300796-rmjv56ia	42	15	(	(	-LRB-
cord-300796-rmjv56ia	42	16	Zerial	Zerial	NNP
cord-300796-rmjv56ia	42	17	et	et	FW
cord-300796-rmjv56ia	42	18	al	al	NNP
cord-300796-rmjv56ia	42	19	.	.	NNP
cord-300796-rmjv56ia	42	20	,	,	,
cord-300796-rmjv56ia	42	21	1986	1986	CD
cord-300796-rmjv56ia	42	22	)	)	-RRB-
cord-300796-rmjv56ia	42	23	with	with	IN
cord-300796-rmjv56ia	42	24	T4	T4	NNP
cord-300796-rmjv56ia	42	25	DNA	DNA	NNP
cord-300796-rmjv56ia	42	26	Polymerase	Polymerase	NNP
cord-300796-rmjv56ia	42	27	was	be	VBD
cord-300796-rmjv56ia	42	28	done	do	VBN
cord-300796-rmjv56ia	42	29	at	at	IN
cord-300796-rmjv56ia	42	30	15	15	CD
cord-300796-rmjv56ia	42	31	°	°	NN
cord-300796-rmjv56ia	42	32	C	c	NN
cord-300796-rmjv56ia	42	33	(	(	-LRB-
cord-300796-rmjv56ia	42	34	2	2	CD
cord-300796-rmjv56ia	42	35	h	h	NNP
cord-300796-rmjv56ia	42	36	)	)	-RRB-
cord-300796-rmjv56ia	42	37	,	,	,
cord-300796-rmjv56ia	42	38	dNTPs	dntp	NNS
cord-300796-rmjv56ia	42	39	were	be	VBD
cord-300796-rmjv56ia	42	40	added	add	VBN
cord-300796-rmjv56ia	42	41	(	(	-LRB-
cord-300796-rmjv56ia	42	42	end	end	NN
cord-300796-rmjv56ia	42	43	concentration	concentration	NN
cord-300796-rmjv56ia	42	44	100	100	CD
cord-300796-rmjv56ia	42	45	#	#	NN
cord-300796-rmjv56ia	42	46	M	m	NN
cord-300796-rmjv56ia	42	47	each	each	DT
cord-300796-rmjv56ia	42	48	)	)	-RRB-
cord-300796-rmjv56ia	42	49	,	,	,
cord-300796-rmjv56ia	42	50	and	and	CC
cord-300796-rmjv56ia	42	51	the	the	DT
cord-300796-rmjv56ia	42	52	DNA	dna	NN
cord-300796-rmjv56ia	42	53	was	be	VBD
cord-300796-rmjv56ia	42	54	subsequently	subsequently	RB
cord-300796-rmjv56ia	42	55	filled	fill	VBN
cord-300796-rmjv56ia	42	56	in	in	RP
cord-300796-rmjv56ia	42	57	at	at	IN
cord-300796-rmjv56ia	42	58	15	15	CD
cord-300796-rmjv56ia	42	59	°	°	NN
cord-300796-rmjv56ia	42	60	C	c	NN
cord-300796-rmjv56ia	42	61	for	for	IN
cord-300796-rmjv56ia	42	62	1	1	CD
cord-300796-rmjv56ia	42	63	h.	h.	NN
cord-300796-rmjv56ia	43	1	All	all	DT
cord-300796-rmjv56ia	43	2	ligations	ligation	NNS
cord-300796-rmjv56ia	43	3	were	be	VBD
cord-300796-rmjv56ia	43	4	done	do	VBN
cord-300796-rmjv56ia	43	5	at	at	IN
cord-300796-rmjv56ia	43	6	24	24	CD
cord-300796-rmjv56ia	43	7	°	°	NN
cord-300796-rmjv56ia	43	8	C	c	NN
cord-300796-rmjv56ia	43	9	for	for	IN
cord-300796-rmjv56ia	43	10	4	4	CD
cord-300796-rmjv56ia	43	11	h	h	NN
cord-300796-rmjv56ia	43	12	except	except	IN
cord-300796-rmjv56ia	43	13	for	for	IN
cord-300796-rmjv56ia	43	14	linker	linker	NN
cord-300796-rmjv56ia	43	15	ligations	ligation	NNS
cord-300796-rmjv56ia	43	16	(	(	-LRB-
cord-300796-rmjv56ia	43	17	4	4	CD
cord-300796-rmjv56ia	43	18	°	°	NN
cord-300796-rmjv56ia	43	19	C	c	NN
cord-300796-rmjv56ia	43	20	,	,	,
cord-300796-rmjv56ia	43	21	16	16	CD
cord-300796-rmjv56ia	43	22	h	h	NN
cord-300796-rmjv56ia	43	23	)	)	-RRB-
cord-300796-rmjv56ia	43	24	.	.	.
cord-300796-rmjv56ia	44	1	All	all	DT
cord-300796-rmjv56ia	44	2	other	other	JJ
cord-300796-rmjv56ia	44	3	molecular	molecular	JJ
cord-300796-rmjv56ia	44	4	biological	biological	JJ
cord-300796-rmjv56ia	44	5	manipulations	manipulation	NNS
cord-300796-rmjv56ia	44	6	were	be	VBD
cord-300796-rmjv56ia	44	7	done	do	VBN
cord-300796-rmjv56ia	44	8	using	use	VBG
cord-300796-rmjv56ia	44	9	slightly	slightly	RB
cord-300796-rmjv56ia	44	10	modified	modify	VBN
cord-300796-rmjv56ia	44	11	standard	standard	JJ
cord-300796-rmjv56ia	44	12	protocols	protocol	NNS
cord-300796-rmjv56ia	44	13	(	(	-LRB-
cord-300796-rmjv56ia	44	14	Maniatis	Maniatis	NNP
cord-300796-rmjv56ia	44	15	et	et	NNP
cord-300796-rmjv56ia	44	16	al	al	NNP
cord-300796-rmjv56ia	44	17	.	.	NNP
cord-300796-rmjv56ia	44	18	,	,	,
cord-300796-rmjv56ia	44	19	1982	1982	CD
cord-300796-rmjv56ia	44	20	)	)	-RRB-
cord-300796-rmjv56ia	44	21	.	.	.
cord-300796-rmjv56ia	45	1	In	in	IN
cord-300796-rmjv56ia	45	2	vitro	vitro	FW
cord-300796-rmjv56ia	45	3	transcription	transcription	NN
cord-300796-rmjv56ia	45	4	(	(	-LRB-
cord-300796-rmjv56ia	45	5	0.3	0.3	CD
cord-300796-rmjv56ia	45	6	#	#	NN
cord-300796-rmjv56ia	45	7	g	g	NN
cord-300796-rmjv56ia	45	8	supercoiled	supercoile	VBN
cord-300796-rmjv56ia	45	9	template	template	NN
cord-300796-rmjv56ia	45	10	DNA	dna	NN
cord-300796-rmjv56ia	45	11	per	per	IN
cord-300796-rmjv56ia	45	12	10	10	CD
cord-300796-rmjv56ia	45	13	#	#	$
cord-300796-rmjv56ia	45	14	1	1	CD
cord-300796-rmjv56ia	45	15	vol	vol	NN
cord-300796-rmjv56ia	45	16	)	)	-RRB-
cord-300796-rmjv56ia	45	17	in	in	IN
cord-300796-rmjv56ia	45	18	the	the	DT
cord-300796-rmjv56ia	45	19	presence	presence	NN
cord-300796-rmjv56ia	45	20	of	of	IN
cord-300796-rmjv56ia	45	21	SP6	SP6	NNP
cord-300796-rmjv56ia	45	22	RNA	RNA	NNP
cord-300796-rmjv56ia	45	23	Polymerase	Polymerase	NNP
cord-300796-rmjv56ia	45	24	(	(	-LRB-
cord-300796-rmjv56ia	45	25	6	6	CD
cord-300796-rmjv56ia	45	26	-	-	SYM
cord-300796-rmjv56ia	45	27	8	8	CD
cord-300796-rmjv56ia	45	28	U	U	NNP
cord-300796-rmjv56ia	45	29	)	)	-RRB-
cord-300796-rmjv56ia	45	30	and	and	CC
cord-300796-rmjv56ia	45	31	the	the	DT
cord-300796-rmjv56ia	45	32	cap	cap	NN
cord-300796-rmjv56ia	45	33	structure	structure	NN
cord-300796-rmjv56ia	45	34	was	be	VBD
cord-300796-rmjv56ia	45	35	carried	carry	VBN
cord-300796-rmjv56ia	45	36	out	out	RP
cord-300796-rmjv56ia	45	37	as	as	IN
cord-300796-rmjv56ia	45	38	previously	previously	RB
cord-300796-rmjv56ia	45	39	described	describe	VBN
cord-300796-rmjv56ia	45	40	(	(	-LRB-
cord-300796-rmjv56ia	45	41	Zerial	Zerial	NNP
cord-300796-rmjv56ia	45	42	et	et	FW
cord-300796-rmjv56ia	45	43	al	al	NNP
cord-300796-rmjv56ia	45	44	.	.	NNP
cord-300796-rmjv56ia	45	45	,	,	,
cord-300796-rmjv56ia	45	46	1986	1986	CD
cord-300796-rmjv56ia	45	47	)	)	-RRB-
cord-300796-rmjv56ia	45	48	.	.	.
cord-300796-rmjv56ia	46	1	In	in	IN
cord-300796-rmjv56ia	46	2	vitro	vitro	FW
cord-300796-rmjv56ia	46	3	translation	translation	NN
cord-300796-rmjv56ia	46	4	reactions	reaction	NNS
cord-300796-rmjv56ia	46	5	using	use	VBG
cord-300796-rmjv56ia	46	6	a	a	DT
cord-300796-rmjv56ia	46	7	rabbit	rabbit	NN
cord-300796-rmjv56ia	46	8	reticulocyte	reticulocyte	NN
cord-300796-rmjv56ia	46	9	lysate	lysate	NN
cord-300796-rmjv56ia	46	10	were	be	VBD
cord-300796-rmjv56ia	46	11	performed	perform	VBN
cord-300796-rmjv56ia	46	12	at	at	IN
cord-300796-rmjv56ia	46	13	30	30	CD
cord-300796-rmjv56ia	46	14	°	°	NN
cord-300796-rmjv56ia	46	15	C	c	NN
cord-300796-rmjv56ia	46	16	essentially	essentially	RB
cord-300796-rmjv56ia	46	17	as	as	IN
cord-300796-rmjv56ia	46	18	described	describe	VBN
cord-300796-rmjv56ia	46	19	.	.	.
cord-300796-rmjv56ia	47	1	1.5	1.5	CD
cord-300796-rmjv56ia	47	2	#	#	$
cord-300796-rmjv56ia	47	3	1	1	CD
cord-300796-rmjv56ia	47	4	of	of	IN
cord-300796-rmjv56ia	47	5	the	the	DT
cord-300796-rmjv56ia	47	6	in	in	FW
cord-300796-rmjv56ia	47	7	vitro	vitro	FW
cord-300796-rmjv56ia	47	8	synthesized	synthesize	VBN
cord-300796-rmjv56ia	47	9	RNA	RNA	NNP
cord-300796-rmjv56ia	47	10	was	be	VBD
cord-300796-rmjv56ia	47	11	translated	translate	VBN
cord-300796-rmjv56ia	47	12	in	in	IN
cord-300796-rmjv56ia	47	13	a	a	DT
cord-300796-rmjv56ia	47	14	total	total	JJ
cord-300796-rmjv56ia	47	15	volume	volume	NN
cord-300796-rmjv56ia	47	16	of	of	IN
cord-300796-rmjv56ia	47	17	15	15	CD
cord-300796-rmjv56ia	47	18	#	#	$
cord-300796-rmjv56ia	47	19	1	1	CD
cord-300796-rmjv56ia	47	20	.	.	.
cord-300796-rmjv56ia	48	1	Potassium	potassium	NN
cord-300796-rmjv56ia	48	2	,	,	,
cord-300796-rmjv56ia	48	3	magnesium	magnesium	NN
cord-300796-rmjv56ia	48	4	,	,	,
cord-300796-rmjv56ia	48	5	and	and	CC
cord-300796-rmjv56ia	48	6	spermidine	spermidine	NN
cord-300796-rmjv56ia	48	7	concentrations	concentration	NNS
cord-300796-rmjv56ia	48	8	were	be	VBD
cord-300796-rmjv56ia	48	9	100	100	CD
cord-300796-rmjv56ia	48	10	,	,	,
cord-300796-rmjv56ia	48	11	1.2	1.2	CD
cord-300796-rmjv56ia	48	12	,	,	,
cord-300796-rmjv56ia	48	13	and	and	CC
cord-300796-rmjv56ia	48	14	0.375	0.375	CD
cord-300796-rmjv56ia	48	15	mM	mm	CD
cord-300796-rmjv56ia	48	16	,	,	,
cord-300796-rmjv56ia	48	17	respectively	respectively	RB
cord-300796-rmjv56ia	48	18	.	.	.
cord-300796-rmjv56ia	49	1	When	when	WRB
cord-300796-rmjv56ia	49	2	indicated	indicate	VBN
cord-300796-rmjv56ia	49	3	,	,	,
cord-300796-rmjv56ia	49	4	1	1	CD
cord-300796-rmjv56ia	49	5	#	#	$
cord-300796-rmjv56ia	49	6	1	1	CD
cord-300796-rmjv56ia	49	7	of	of	IN
cord-300796-rmjv56ia	49	8	ER	ER	NNP
cord-300796-rmjv56ia	49	9	membranes	membrane	NNS
cord-300796-rmjv56ia	49	10	was	be	VBD
cord-300796-rmjv56ia	49	11	included	include	VBN
cord-300796-rmjv56ia	49	12	.	.	.
cord-300796-rmjv56ia	50	1	In	in	IN
cord-300796-rmjv56ia	50	2	some	some	DT
cord-300796-rmjv56ia	50	3	translocations	translocation	NNS
cord-300796-rmjv56ia	50	4	the	the	DT
cord-300796-rmjv56ia	50	5	membranes	membrane	NNS
cord-300796-rmjv56ia	50	6	were	be	VBD
cord-300796-rmjv56ia	50	7	pretreated	pretreate	VBN
cord-300796-rmjv56ia	50	8	with	with	IN
cord-300796-rmjv56ia	50	9	200	200	CD
cord-300796-rmjv56ia	50	10	#	#	NN
cord-300796-rmjv56ia	50	11	M	m	NN
cord-300796-rmjv56ia	50	12	peptide	peptide	NN
cord-300796-rmjv56ia	50	13	for	for	IN
cord-300796-rmjv56ia	50	14	5	5	CD
cord-300796-rmjv56ia	50	15	min	min	NN
cord-300796-rmjv56ia	50	16	on	on	IN
cord-300796-rmjv56ia	50	17	ice	ice	NN
cord-300796-rmjv56ia	50	18	.	.	.
cord-300796-rmjv56ia	51	1	The	the	DT
cord-300796-rmjv56ia	51	2	final	final	JJ
cord-300796-rmjv56ia	51	3	peptide	peptide	NN
cord-300796-rmjv56ia	51	4	concentration	concentration	NN
cord-300796-rmjv56ia	51	5	in	in	IN
cord-300796-rmjv56ia	51	6	the	the	DT
cord-300796-rmjv56ia	51	7	total	total	JJ
cord-300796-rmjv56ia	51	8	translation	translation	NN
cord-300796-rmjv56ia	51	9	mixture	mixture	NN
cord-300796-rmjv56ia	51	10	here	here	RB
cord-300796-rmjv56ia	51	11	was	be	VBD
cord-300796-rmjv56ia	51	12	,	,	,
cord-300796-rmjv56ia	51	13	after	after	IN
cord-300796-rmjv56ia	51	14	addition	addition	NN
cord-300796-rmjv56ia	51	15	of	of	IN
cord-300796-rmjv56ia	51	16	the	the	DT
cord-300796-rmjv56ia	51	17	pretreated	pretreate	VBN
cord-300796-rmjv56ia	51	18	membranes	membrane	NNS
cord-300796-rmjv56ia	51	19	,	,	,
cord-300796-rmjv56ia	51	20	adjusted	adjust	VBN
cord-300796-rmjv56ia	51	21	to	to	IN
cord-300796-rmjv56ia	51	22	100/~M.	100/~m.	CD
cord-300796-rmjv56ia	51	23	To	to	TO
cord-300796-rmjv56ia	51	24	obtain	obtain	VB
cord-300796-rmjv56ia	51	25	partial	partial	JJ
cord-300796-rmjv56ia	51	26	synchronization	synchronization	NN
cord-300796-rmjv56ia	51	27	of	of	IN
cord-300796-rmjv56ia	51	28	translation	translation	NN
cord-300796-rmjv56ia	51	29	,	,	,
cord-300796-rmjv56ia	51	30	ATA	ATA	NNP
cord-300796-rmjv56ia	51	31	was	be	VBD
cord-300796-rmjv56ia	51	32	added	add	VBN
cord-300796-rmjv56ia	51	33	after	after	IN
cord-300796-rmjv56ia	51	34	a	a	DT
cord-300796-rmjv56ia	51	35	preincubation	preincubation	NN
cord-300796-rmjv56ia	51	36	of	of	IN
cord-300796-rmjv56ia	51	37	1.5	1.5	CD
cord-300796-rmjv56ia	51	38	-	-	SYM
cord-300796-rmjv56ia	51	39	3.0	3.0	CD
cord-300796-rmjv56ia	51	40	rain	rain	NN
cord-300796-rmjv56ia	51	41	(	(	-LRB-
cord-300796-rmjv56ia	51	42	Borgese	Borgese	NNP
cord-300796-rmjv56ia	51	43	et	et	NNP
cord-300796-rmjv56ia	51	44	al	al	NNP
cord-300796-rmjv56ia	51	45	.	.	NNP
cord-300796-rmjv56ia	51	46	,	,	,
cord-300796-rmjv56ia	51	47	1974	1974	CD
cord-300796-rmjv56ia	51	48	)	)	-RRB-
cord-300796-rmjv56ia	51	49	.	.	.
cord-300796-rmjv56ia	52	1	A	a	DT
cord-300796-rmjv56ia	52	2	final	final	JJ
cord-300796-rmjv56ia	52	3	ATA	ATA	NNP
cord-300796-rmjv56ia	52	4	concentration	concentration	NN
cord-300796-rmjv56ia	52	5	of	of	IN
cord-300796-rmjv56ia	52	6	0.075	0.075	CD
cord-300796-rmjv56ia	52	7	mM	mm	NN
cord-300796-rmjv56ia	52	8	was	be	VBD
cord-300796-rmjv56ia	52	9	found	find	VBN
cord-300796-rmjv56ia	52	10	to	to	TO
cord-300796-rmjv56ia	52	11	be	be	VB
cord-300796-rmjv56ia	52	12	sufficient	sufficient	JJ
cord-300796-rmjv56ia	52	13	for	for	IN
cord-300796-rmjv56ia	52	14	inhibiting	inhibit	VBG
cord-300796-rmjv56ia	52	15	initiation	initiation	NN
cord-300796-rmjv56ia	52	16	of	of	IN
cord-300796-rmjv56ia	52	17	chain	chain	NN
cord-300796-rmjv56ia	52	18	synthesis	synthesis	NN
cord-300796-rmjv56ia	52	19	(	(	-LRB-
cord-300796-rmjv56ia	52	20	see	see	VB
cord-300796-rmjv56ia	52	21	control	control	NN
cord-300796-rmjv56ia	52	22	in	in	IN
cord-300796-rmjv56ia	52	23	Fig	Fig	NNP
cord-300796-rmjv56ia	52	24	.	.	NNP
cord-300796-rmjv56ia	52	25	6	6	CD
cord-300796-rmjv56ia	52	26	,	,	,
cord-300796-rmjv56ia	52	27	lane/	lane/	CD
cord-300796-rmjv56ia	52	28	)	)	-RRB-
cord-300796-rmjv56ia	52	29	.	.	.
cord-300796-rmjv56ia	53	1	Higher	high	JJR
cord-300796-rmjv56ia	53	2	concentrations	concentration	NNS
cord-300796-rmjv56ia	53	3	of	of	IN
cord-300796-rmjv56ia	53	4	ATA	ATA	NNP
cord-300796-rmjv56ia	53	5	inhibited	inhibit	VBD
cord-300796-rmjv56ia	53	6	first	first	JJ
cord-300796-rmjv56ia	53	7	transloeation	transloeation	NN
cord-300796-rmjv56ia	53	8	and	and	CC
cord-300796-rmjv56ia	53	9	then	then	RB
cord-300796-rmjv56ia	53	10	also	also	RB
cord-300796-rmjv56ia	53	11	chain	chain	VBP
cord-300796-rmjv56ia	53	12	elongation	elongation	NN
cord-300796-rmjv56ia	53	13	.	.	.
cord-300796-rmjv56ia	54	1	For	for	IN
cord-300796-rmjv56ia	54	2	protease	protease	NN
cord-300796-rmjv56ia	54	3	protection	protection	NN
cord-300796-rmjv56ia	54	4	experiments	experiment	NNS
cord-300796-rmjv56ia	54	5	,	,	,
cord-300796-rmjv56ia	54	6	proteinase	proteinase	NN
cord-300796-rmjv56ia	54	7	K	K	NNP
cord-300796-rmjv56ia	54	8	was	be	VBD
cord-300796-rmjv56ia	54	9	added	add	VBN
cord-300796-rmjv56ia	54	10	to	to	IN
cord-300796-rmjv56ia	54	11	a	a	DT
cord-300796-rmjv56ia	54	12	final	final	JJ
cord-300796-rmjv56ia	54	13	concentration	concentration	NN
cord-300796-rmjv56ia	54	14	of	of	IN
cord-300796-rmjv56ia	54	15	0.1	0.1	CD
cord-300796-rmjv56ia	54	16	mg	mg	NNP
cord-300796-rmjv56ia	54	17	/	/	SYM
cord-300796-rmjv56ia	54	18	ml	ml	NN
cord-300796-rmjv56ia	54	19	and	and	CC
cord-300796-rmjv56ia	54	20	the	the	DT
cord-300796-rmjv56ia	54	21	samples	sample	NNS
cord-300796-rmjv56ia	54	22	were	be	VBD
cord-300796-rmjv56ia	54	23	incubated	incubate	VBN
cord-300796-rmjv56ia	54	24	at	at	IN
cord-300796-rmjv56ia	54	25	0*C	0*c	CD
cord-300796-rmjv56ia	54	26	for	for	IN
cord-300796-rmjv56ia	54	27	30	30	CD
cord-300796-rmjv56ia	54	28	min	min	NN
cord-300796-rmjv56ia	54	29	in	in	IN
cord-300796-rmjv56ia	54	30	the	the	DT
cord-300796-rmjv56ia	54	31	presence	presence	NN
cord-300796-rmjv56ia	54	32	or	or	CC
cord-300796-rmjv56ia	54	33	absence	absence	NN
cord-300796-rmjv56ia	54	34	of	of	IN
cord-300796-rmjv56ia	54	35	1	1	CD
cord-300796-rmjv56ia	54	36	%	%	NN
cord-300796-rmjv56ia	54	37	Triton	Triton	NNP
cord-300796-rmjv56ia	54	38	X-100	x-100	RB
cord-300796-rmjv56ia	54	39	.	.	.
cord-300796-rmjv56ia	55	1	Proteolysis	Proteolysis	NNP
cord-300796-rmjv56ia	55	2	was	be	VBD
cord-300796-rmjv56ia	55	3	stopped	stop	VBN
cord-300796-rmjv56ia	55	4	by	by	IN
cord-300796-rmjv56ia	55	5	the	the	DT
cord-300796-rmjv56ia	55	6	addition	addition	NN
cord-300796-rmjv56ia	55	7	of	of	IN
cord-300796-rmjv56ia	55	8	PMSF	PMSF	NNP
cord-300796-rmjv56ia	55	9	(	(	-LRB-
cord-300796-rmjv56ia	55	10	final	final	JJ
cord-300796-rmjv56ia	55	11	concentration	concentration	NN
cord-300796-rmjv56ia	55	12	2	2	CD
cord-300796-rmjv56ia	55	13	mg	mg	NNP
cord-300796-rmjv56ia	55	14	/	/	SYM
cord-300796-rmjv56ia	55	15	ml	ml	NN
cord-300796-rmjv56ia	55	16	)	)	-RRB-
cord-300796-rmjv56ia	55	17	and	and	CC
cord-300796-rmjv56ia	55	18	samples	sample	NNS
cord-300796-rmjv56ia	55	19	were	be	VBD
cord-300796-rmjv56ia	55	20	kept	keep	VBN
cord-300796-rmjv56ia	55	21	at	at	IN
cord-300796-rmjv56ia	55	22	0*C	0*c	CD
cord-300796-rmjv56ia	55	23	for	for	IN
cord-300796-rmjv56ia	55	24	5	5	CD
cord-300796-rmjv56ia	55	25	min	min	NN
cord-300796-rmjv56ia	55	26	before	before	IN
cord-300796-rmjv56ia	55	27	further	further	JJ
cord-300796-rmjv56ia	55	28	processing	processing	NN
cord-300796-rmjv56ia	55	29	for	for	IN
cord-300796-rmjv56ia	55	30	electrophoresis	electrophoresis	NN
cord-300796-rmjv56ia	55	31	(	(	-LRB-
cord-300796-rmjv56ia	55	32	Cutler	Cutler	NNP
cord-300796-rmjv56ia	55	33	and	and	CC
cord-300796-rmjv56ia	55	34	Garoff	Garoff	NNP
cord-300796-rmjv56ia	55	35	,	,	,
cord-300796-rmjv56ia	55	36	1986	1986	CD
cord-300796-rmjv56ia	55	37	)	)	-RRB-
cord-300796-rmjv56ia	55	38	.	.	.
cord-300796-rmjv56ia	56	1	Bands	band	NNS
cord-300796-rmjv56ia	56	2	containing	contain	VBG
cord-300796-rmjv56ia	56	3	labeled	label	VBN
cord-300796-rmjv56ia	56	4	protein	protein	NN
cord-300796-rmjv56ia	56	5	were	be	VBD
cord-300796-rmjv56ia	56	6	visualized	visualize	VBN
cord-300796-rmjv56ia	56	7	by	by	IN
cord-300796-rmjv56ia	56	8	fluorography	fluorography	NNP
cord-300796-rmjv56ia	56	9	.	.	.
cord-300796-rmjv56ia	57	1	Quantitation	quantitation	NN
cord-300796-rmjv56ia	57	2	of	of	IN
cord-300796-rmjv56ia	57	3	proteins	protein	NNS
cord-300796-rmjv56ia	57	4	was	be	VBD
cord-300796-rmjv56ia	57	5	done	do	VBN
cord-300796-rmjv56ia	57	6	by	by	IN
cord-300796-rmjv56ia	57	7	cutting	cut	VBG
cord-300796-rmjv56ia	57	8	the	the	DT
cord-300796-rmjv56ia	57	9	bands	band	NNS
cord-300796-rmjv56ia	57	10	out	out	IN
cord-300796-rmjv56ia	57	11	of	of	IN
cord-300796-rmjv56ia	57	12	the	the	DT
cord-300796-rmjv56ia	57	13	dried	dry	VBN
cord-300796-rmjv56ia	57	14	gel	gel	NN
cord-300796-rmjv56ia	57	15	,	,	,
cord-300796-rmjv56ia	57	16	solubilizing	solubilize	VBG
cord-300796-rmjv56ia	57	17	them	-PRON-	PRP
cord-300796-rmjv56ia	57	18	with	with	IN
cord-300796-rmjv56ia	57	19	Protosol	Protosol	NNP
cord-300796-rmjv56ia	57	20	(	(	-LRB-
cord-300796-rmjv56ia	57	21	from	from	IN
cord-300796-rmjv56ia	57	22	DuPont	DuPont	NNP
cord-300796-rmjv56ia	57	23	de	de	NNP
cord-300796-rmjv56ia	57	24	Nemours	Nemours	NNP
cord-300796-rmjv56ia	57	25	,	,	,
cord-300796-rmjv56ia	57	26	NEN	NEN	NNP
cord-300796-rmjv56ia	57	27	)	)	-RRB-
cord-300796-rmjv56ia	57	28	according	accord	VBG
cord-300796-rmjv56ia	57	29	to	to	IN
cord-300796-rmjv56ia	57	30	the	the	DT
cord-300796-rmjv56ia	57	31	instructions	instruction	NNS
cord-300796-rmjv56ia	57	32	of	of	IN
cord-300796-rmjv56ia	57	33	the	the	DT
cord-300796-rmjv56ia	57	34	manufacturer	manufacturer	NN
cord-300796-rmjv56ia	57	35	,	,	,
cord-300796-rmjv56ia	57	36	and	and	CC
cord-300796-rmjv56ia	57	37	finally	finally	RB
cord-300796-rmjv56ia	57	38	counting	count	VBG
cord-300796-rmjv56ia	57	39	the	the	DT
cord-300796-rmjv56ia	57	40	3~S	3~s	CD
cord-300796-rmjv56ia	57	41	radioactivity	radioactivity	NN
cord-300796-rmjv56ia	57	42	in	in	IN
cord-300796-rmjv56ia	57	43	a	a	DT
cord-300796-rmjv56ia	57	44	liquid	liquid	JJ
cord-300796-rmjv56ia	57	45	scintillator	scintillator	NN
cord-300796-rmjv56ia	58	1	(	(	-LRB-
cord-300796-rmjv56ia	58	2	Wallac	Wallac	NNP
cord-300796-rmjv56ia	58	3	LKB	LKB	NNP
cord-300796-rmjv56ia	58	4	,	,	,
cord-300796-rmjv56ia	58	5	Turku	Turku	NNP
cord-300796-rmjv56ia	58	6	,	,	,
cord-300796-rmjv56ia	58	7	Finland	Finland	NNP
cord-300796-rmjv56ia	58	8	)	)	-RRB-
cord-300796-rmjv56ia	58	9	.	.	.
cord-300796-rmjv56ia	59	1	The	the	DT
cord-300796-rmjv56ia	59	2	localization	localization	NN
cord-300796-rmjv56ia	59	3	of	of	IN
cord-300796-rmjv56ia	59	4	the	the	DT
cord-300796-rmjv56ia	59	5	bands	band	NNS
cord-300796-rmjv56ia	59	6	on	on	IN
cord-300796-rmjv56ia	59	7	the	the	DT
cord-300796-rmjv56ia	59	8	dried	dry	VBN
cord-300796-rmjv56ia	59	9	gel	gel	NN
cord-300796-rmjv56ia	59	10	was	be	VBD
cord-300796-rmjv56ia	59	11	done	do	VBN
cord-300796-rmjv56ia	59	12	with	with	IN
cord-300796-rmjv56ia	59	13	the	the	DT
cord-300796-rmjv56ia	59	14	aid	aid	NN
cord-300796-rmjv56ia	59	15	of	of	IN
cord-300796-rmjv56ia	59	16	the	the	DT
cord-300796-rmjv56ia	59	17	fluorograph	fluorograph	NN
cord-300796-rmjv56ia	59	18	in	in	IN
cord-300796-rmjv56ia	59	19	transillumination	transillumination	NN
cord-300796-rmjv56ia	59	20	.	.	.
cord-300796-rmjv56ia	60	1	15-#1	15-#1	CD
cord-300796-rmjv56ia	60	2	translation	translation	NN
cord-300796-rmjv56ia	60	3	mixtures	mixture	NNS
cord-300796-rmjv56ia	60	4	were	be	VBD
cord-300796-rmjv56ia	60	5	adjusted	adjust	VBN
cord-300796-rmjv56ia	60	6	to	to	IN
cord-300796-rmjv56ia	60	7	pH	pH	NNP
cord-300796-rmjv56ia	60	8	11	11	CD
cord-300796-rmjv56ia	60	9	-	-	SYM
cord-300796-rmjv56ia	60	10	11.5	11.5	CD
cord-300796-rmjv56ia	60	11	by	by	IN
cord-300796-rmjv56ia	60	12	adding	add	VBG
cord-300796-rmjv56ia	60	13	an	an	DT
cord-300796-rmjv56ia	60	14	appropriate	appropriate	JJ
cord-300796-rmjv56ia	60	15	volume	volume	NN
cord-300796-rmjv56ia	60	16	(	(	-LRB-
cord-300796-rmjv56ia	60	17	pretitrated	pretitrated	JJ
cord-300796-rmjv56ia	60	18	)	)	-RRB-
cord-300796-rmjv56ia	60	19	of	of	IN
cord-300796-rmjv56ia	60	20	0.1	0.1	CD
cord-300796-rmjv56ia	60	21	N	n	CD
cord-300796-rmjv56ia	60	22	NaOH	NaOH	NNP
cord-300796-rmjv56ia	60	23	.	.	.
cord-300796-rmjv56ia	61	1	After	after	IN
cord-300796-rmjv56ia	61	2	a	a	DT
cord-300796-rmjv56ia	61	3	10-rain	10-rain	CD
cord-300796-rmjv56ia	61	4	incubation	incubation	NN
cord-300796-rmjv56ia	61	5	on	on	IN
cord-300796-rmjv56ia	61	6	ice	ice	NN
cord-300796-rmjv56ia	61	7	the	the	DT
cord-300796-rmjv56ia	61	8	samples	sample	NNS
cord-300796-rmjv56ia	61	9	were	be	VBD
cord-300796-rmjv56ia	61	10	separated	separate	VBN
cord-300796-rmjv56ia	61	11	into	into	IN
cord-300796-rmjv56ia	61	12	a	a	DT
cord-300796-rmjv56ia	61	13	pellet	pellet	NN
cord-300796-rmjv56ia	61	14	fraction	fraction	NN
cord-300796-rmjv56ia	61	15	and	and	CC
cord-300796-rmjv56ia	61	16	a	a	DT
cord-300796-rmjv56ia	61	17	supernatant	supernatant	JJ
cord-300796-rmjv56ia	61	18	fraction	fraction	NN
cord-300796-rmjv56ia	61	19	by	by	IN
cord-300796-rmjv56ia	61	20	centrifugation	centrifugation	NN
cord-300796-rmjv56ia	61	21	through	through	IN
cord-300796-rmjv56ia	61	22	a	a	DT
cord-300796-rmjv56ia	61	23	100-#1	100-#1	CD
cord-300796-rmjv56ia	61	24	alkaline	alkaline	JJ
cord-300796-rmjv56ia	61	25	socmse	socmse	JJ
cord-300796-rmjv56ia	61	26	cushion	cushion	NN
cord-300796-rmjv56ia	61	27	(	(	-LRB-
cord-300796-rmjv56ia	61	28	Gilmore	Gilmore	NNP
cord-300796-rmjv56ia	61	29	and	and	CC
cord-300796-rmjv56ia	61	30	Blobel	Blobel	NNP
cord-300796-rmjv56ia	61	31	,	,	,
cord-300796-rmjv56ia	61	32	1985	1985	CD
cord-300796-rmjv56ia	61	33	)	)	-RRB-
cord-300796-rmjv56ia	61	34	for	for	IN
cord-300796-rmjv56ia	61	35	10	10	CD
cord-300796-rmjv56ia	61	36	rain	rain	NN
cord-300796-rmjv56ia	61	37	at	at	IN
cord-300796-rmjv56ia	61	38	30	30	CD
cord-300796-rmjv56ia	61	39	psi	psi	NN
cord-300796-rmjv56ia	61	40	in	in	IN
cord-300796-rmjv56ia	61	41	an	an	DT
cord-300796-rmjv56ia	61	42	airfuge	airfuge	JJ
cord-300796-rmjv56ia	61	43	(	(	-LRB-
cord-300796-rmjv56ia	61	44	Beckman	Beckman	NNP
cord-300796-rmjv56ia	61	45	Instruments	Instruments	NNP
cord-300796-rmjv56ia	61	46	,	,	,
cord-300796-rmjv56ia	61	47	Inc.	Inc.	NNP
cord-300796-rmjv56ia	61	48	,	,	,
cord-300796-rmjv56ia	61	49	Palo	Palo	NNP
cord-300796-rmjv56ia	61	50	Alto	Alto	NNP
cord-300796-rmjv56ia	61	51	,	,	,
cord-300796-rmjv56ia	61	52	CA	CA	NNP
cord-300796-rmjv56ia	61	53	)	)	-RRB-
cord-300796-rmjv56ia	61	54	using	use	VBG
cord-300796-rmjv56ia	61	55	the	the	DT
cord-300796-rmjv56ia	61	56	A-100/30	A-100/30	NNP
cord-300796-rmjv56ia	61	57	rotor	rotor	NN
cord-300796-rmjv56ia	61	58	and	and	CC
cord-300796-rmjv56ia	61	59	cellulose	cellulose	NN
cord-300796-rmjv56ia	61	60	propionate	propionate	NN
cord-300796-rmjv56ia	61	61	tubes	tube	NNS
cord-300796-rmjv56ia	61	62	precoated	precoate	VBN
cord-300796-rmjv56ia	61	63	with	with	IN
cord-300796-rmjv56ia	61	64	BSA	BSA	NNP
cord-300796-rmjv56ia	61	65	(	(	-LRB-
cord-300796-rmjv56ia	61	66	1	1	CD
cord-300796-rmjv56ia	61	67	%	%	NN
cord-300796-rmjv56ia	61	68	solution	solution	NN
cord-300796-rmjv56ia	61	69	)	)	-RRB-
cord-300796-rmjv56ia	61	70	.	.	.
cord-300796-rmjv56ia	62	1	The	the	DT
cord-300796-rmjv56ia	62	2	entire	entire	JJ
cord-300796-rmjv56ia	62	3	supernatant	supernatant	NN
cord-300796-rmjv56ia	62	4	was	be	VBD
cord-300796-rmjv56ia	62	5	removed	remove	VBN
cord-300796-rmjv56ia	62	6	,	,	,
cord-300796-rmjv56ia	62	7	neutralized	neutralize	VBN
cord-300796-rmjv56ia	62	8	with	with	IN
cord-300796-rmjv56ia	62	9	1	1	CD
cord-300796-rmjv56ia	62	10	N	N	NNP
cord-300796-rmjv56ia	62	11	HCI	HCI	NNP
cord-300796-rmjv56ia	62	12	,	,	,
cord-300796-rmjv56ia	62	13	diluted	dilute	VBD
cord-300796-rmjv56ia	62	14	2.5	2.5	CD
cord-300796-rmjv56ia	62	15	times	time	NNS
cord-300796-rmjv56ia	62	16	with	with	IN
cord-300796-rmjv56ia	62	17	water	water	NN
cord-300796-rmjv56ia	62	18	,	,	,
cord-300796-rmjv56ia	62	19	and	and	CC
cord-300796-rmjv56ia	62	20	then	then	RB
cord-300796-rmjv56ia	62	21	precipitated	precipitate	VBN
cord-300796-rmjv56ia	62	22	by	by	IN
cord-300796-rmjv56ia	62	23	adding	add	VBG
cord-300796-rmjv56ia	62	24	3.5	3.5	CD
cord-300796-rmjv56ia	62	25	vol	vol	NN
cord-300796-rmjv56ia	62	26	of	of	IN
cord-300796-rmjv56ia	62	27	acetone	acetone	NN
cord-300796-rmjv56ia	62	28	.	.	.
cord-300796-rmjv56ia	63	1	These	these	DT
cord-300796-rmjv56ia	63	2	precipitated	precipitate	VBN
cord-300796-rmjv56ia	63	3	proteins	protein	NNS
cord-300796-rmjv56ia	63	4	and	and	CC
cord-300796-rmjv56ia	63	5	pelleted	pellete	VBN
cord-300796-rmjv56ia	63	6	membranes	membrane	NNS
cord-300796-rmjv56ia	63	7	(	(	-LRB-
cord-300796-rmjv56ia	63	8	obtained	obtain	VBN
cord-300796-rmjv56ia	63	9	from	from	IN
cord-300796-rmjv56ia	63	10	the	the	DT
cord-300796-rmjv56ia	63	11	airfuge	airfuge	NNP
cord-300796-rmjv56ia	63	12	tube	tube	NN
cord-300796-rmjv56ia	63	13	)	)	-RRB-
cord-300796-rmjv56ia	63	14	were	be	VBD
cord-300796-rmjv56ia	63	15	taken	take	VBN
cord-300796-rmjv56ia	63	16	up	up	RP
cord-300796-rmjv56ia	63	17	in	in	IN
cord-300796-rmjv56ia	63	18	4	4	CD
cord-300796-rmjv56ia	63	19	%	%	NN
cord-300796-rmjv56ia	63	20	SDS	SDS	NNP
cord-300796-rmjv56ia	63	21	by	by	IN
cord-300796-rmjv56ia	63	22	incubating	incubate	VBG
cord-300796-rmjv56ia	63	23	at	at	IN
cord-300796-rmjv56ia	63	24	56	56	CD
cord-300796-rmjv56ia	63	25	°	°	NN
cord-300796-rmjv56ia	63	26	C	c	NN
cord-300796-rmjv56ia	63	27	for	for	IN
cord-300796-rmjv56ia	63	28	15	15	CD
cord-300796-rmjv56ia	63	29	min	min	NN
cord-300796-rmjv56ia	63	30	and	and	CC
cord-300796-rmjv56ia	63	31	then	then	RB
cord-300796-rmjv56ia	63	32	processed	process	VBN
cord-300796-rmjv56ia	63	33	for	for	IN
cord-300796-rmjv56ia	63	34	immunoprecipitation	immunoprecipitation	NN
cord-300796-rmjv56ia	63	35	reactions	reaction	NNS
cord-300796-rmjv56ia	63	36	as	as	IN
cord-300796-rmjv56ia	63	37	described	describe	VBN
cord-300796-rmjv56ia	63	38	below	below	RB
cord-300796-rmjv56ia	63	39	.	.	.
cord-300796-rmjv56ia	64	1	Total	total	JJ
cord-300796-rmjv56ia	64	2	translation	translation	NN
cord-300796-rmjv56ia	64	3	mixtures	mixture	NNS
cord-300796-rmjv56ia	64	4	were	be	VBD
cord-300796-rmjv56ia	64	5	adjusted	adjust	VBN
cord-300796-rmjv56ia	64	6	to	to	IN
cord-300796-rmjv56ia	64	7	4	4	CD
cord-300796-rmjv56ia	64	8	%	%	NN
cord-300796-rmjv56ia	64	9	SDS	SDS	NNP
cord-300796-rmjv56ia	64	10	,	,	,
cord-300796-rmjv56ia	64	11	then	then	RB
cord-300796-rmjv56ia	64	12	boiled	boil	VBD
cord-300796-rmjv56ia	64	13	for	for	IN
cord-300796-rmjv56ia	64	14	4	4	CD
cord-300796-rmjv56ia	64	15	rain	rain	NN
cord-300796-rmjv56ia	64	16	and	and	CC
cord-300796-rmjv56ia	64	17	diluted	dilute	VBN
cord-300796-rmjv56ia	64	18	1:2	1:2	CD
cord-300796-rmjv56ia	64	19	with	with	IN
cord-300796-rmjv56ia	64	20	water	water	NN
cord-300796-rmjv56ia	64	21	.	.	.
cord-300796-rmjv56ia	65	1	4	4	LS
cord-300796-rmjv56ia	65	2	vol	vol	NN
cord-300796-rmjv56ia	65	3	of	of	IN
cord-300796-rmjv56ia	65	4	immunoprecipitation	immunoprecipitation	NN
cord-300796-rmjv56ia	65	5	buffer	buffer	NN
cord-300796-rmjv56ia	65	6	(	(	-LRB-
cord-300796-rmjv56ia	65	7	2.5	2.5	CD
cord-300796-rmjv56ia	65	8	%	%	NN
cord-300796-rmjv56ia	65	9	Triton	Triton	NNP
cord-300796-rmjv56ia	65	10	X-100	x-100	NN
cord-300796-rmjv56ia	65	11	,	,	,
cord-300796-rmjv56ia	65	12	190	190	CD
cord-300796-rmjv56ia	65	13	mM	mm	CD
cord-300796-rmjv56ia	65	14	NaC1	NaC1	NNPS
cord-300796-rmjv56ia	65	15	,	,	,
cord-300796-rmjv56ia	65	16	60	60	CD
cord-300796-rmjv56ia	65	17	mM	mM	NNP
cord-300796-rmjv56ia	65	18	Tris	Tris	NNP
cord-300796-rmjv56ia	65	19	-	-	HYPH
cord-300796-rmjv56ia	65	20	HC1	HC1	NNP
cord-300796-rmjv56ia	65	21	,	,	,
cord-300796-rmjv56ia	65	22	pH	pH	NNP
cord-300796-rmjv56ia	65	23	7.4	7.4	CD
cord-300796-rmjv56ia	65	24	,	,	,
cord-300796-rmjv56ia	65	25	6	6	CD
cord-300796-rmjv56ia	65	26	mM	mm	CD
cord-300796-rmjv56ia	65	27	EDTA	EDTA	NNP
cord-300796-rmjv56ia	65	28	,	,	,
cord-300796-rmjv56ia	65	29	and	and	CC
cord-300796-rmjv56ia	65	30	20/~g	20/~g	NNP
cord-300796-rmjv56ia	65	31	PMSF	PMSF	NNP
cord-300796-rmjv56ia	65	32	/	/	SYM
cord-300796-rmjv56ia	65	33	rni	rni	NNP
cord-300796-rmjv56ia	65	34	)	)	-RRB-
cord-300796-rmjv56ia	65	35	and	and	CC
cord-300796-rmjv56ia	65	36	2	2	CD
cord-300796-rmjv56ia	65	37	#	#	$
cord-300796-rmjv56ia	65	38	1	1	CD
cord-300796-rmjv56ia	65	39	of	of	IN
cord-300796-rmjv56ia	65	40	antibody	antibody	NN
cord-300796-rmjv56ia	65	41	were	be	VBD
cord-300796-rmjv56ia	65	42	added	add	VBN
cord-300796-rmjv56ia	65	43	for	for	IN
cord-300796-rmjv56ia	65	44	16	16	CD
cord-300796-rmjv56ia	65	45	h	h	NN
cord-300796-rmjv56ia	65	46	at	at	IN
cord-300796-rmjv56ia	65	47	40C.	40c.	CD
cord-300796-rmjv56ia	66	1	The	the	DT
cord-300796-rmjv56ia	66	2	mixture	mixture	NN
cord-300796-rmjv56ia	66	3	was	be	VBD
cord-300796-rmjv56ia	66	4	briefly	briefly	RB
cord-300796-rmjv56ia	66	5	centrifuged	centrifuge	VBN
cord-300796-rmjv56ia	66	6	(	(	-LRB-
cord-300796-rmjv56ia	66	7	2	2	CD
cord-300796-rmjv56ia	66	8	-	-	SYM
cord-300796-rmjv56ia	66	9	3	3	CD
cord-300796-rmjv56ia	66	10	min	min	NN
cord-300796-rmjv56ia	66	11	in	in	IN
cord-300796-rmjv56ia	66	12	an	an	DT
cord-300796-rmjv56ia	66	13	Eppendorf	Eppendorf	NNP
cord-300796-rmjv56ia	66	14	minifuge	minifuge	NN
cord-300796-rmjv56ia	66	15	)	)	-RRB-
cord-300796-rmjv56ia	66	16	and	and	CC
cord-300796-rmjv56ia	66	17	to	to	IN
cord-300796-rmjv56ia	66	18	the	the	DT
cord-300796-rmjv56ia	66	19	supernatant	supernatant	JJ
cord-300796-rmjv56ia	66	20	one	one	CD
cord-300796-rmjv56ia	66	21	fifth	fifth	JJ
cord-300796-rmjv56ia	66	22	volume	volume	NN
cord-300796-rmjv56ia	66	23	of	of	IN
cord-300796-rmjv56ia	66	24	a	a	DT
cord-300796-rmjv56ia	66	25	1:1	1:1	CD
cord-300796-rmjv56ia	66	26	slurry	slurry	NN
cord-300796-rmjv56ia	66	27	of	of	IN
cord-300796-rmjv56ia	66	28	protein	protein	NN
cord-300796-rmjv56ia	67	1	A	a	DT
cord-300796-rmjv56ia	67	2	beads	bead	NNS
cord-300796-rmjv56ia	67	3	were	be	VBD
cord-300796-rmjv56ia	67	4	added	add	VBN
cord-300796-rmjv56ia	67	5	and	and	CC
cord-300796-rmjv56ia	67	6	incubated	incubate	VBN
cord-300796-rmjv56ia	67	7	at	at	IN
cord-300796-rmjv56ia	67	8	24"C	24"c	CD
cord-300796-rmjv56ia	67	9	for	for	IN
cord-300796-rmjv56ia	67	10	2	2	CD
cord-300796-rmjv56ia	67	11	h	h	NN
cord-300796-rmjv56ia	67	12	under	under	IN
cord-300796-rmjv56ia	67	13	constant	constant	JJ
cord-300796-rmjv56ia	67	14	agitation	agitation	NN
cord-300796-rmjv56ia	67	15	.	.	.
cord-300796-rmjv56ia	68	1	The	the	DT
cord-300796-rmjv56ia	68	2	beads	bead	NNS
cord-300796-rmjv56ia	68	3	were	be	VBD
cord-300796-rmjv56ia	68	4	collected	collect	VBN
cord-300796-rmjv56ia	68	5	and	and	CC
cord-300796-rmjv56ia	68	6	washed	wash	VBD
cord-300796-rmjv56ia	68	7	four	four	CD
cord-300796-rmjv56ia	68	8	times	time	NNS
cord-300796-rmjv56ia	68	9	with	with	IN
cord-300796-rmjv56ia	68	10	1	1	CD
cord-300796-rmjv56ia	68	11	ml	ml	NN
cord-300796-rmjv56ia	68	12	RIPA	RIPA	NNP
cord-300796-rmjv56ia	68	13	buffer	buffer	NN
cord-300796-rmjv56ia	68	14	(	(	-LRB-
cord-300796-rmjv56ia	68	15	Gielkens	Gielkens	NNP
cord-300796-rmjv56ia	68	16	et	et	FW
cord-300796-rmjv56ia	68	17	al	al	NNP
cord-300796-rmjv56ia	68	18	.	.	NNP
cord-300796-rmjv56ia	68	19	,	,	,
cord-300796-rmjv56ia	68	20	1976	1976	CD
cord-300796-rmjv56ia	68	21	)	)	-RRB-
cord-300796-rmjv56ia	68	22	by	by	IN
cord-300796-rmjv56ia	68	23	centrifugation	centrifugation	NN
cord-300796-rmjv56ia	68	24	,	,	,
cord-300796-rmjv56ia	68	25	followed	follow	VBN
cord-300796-rmjv56ia	68	26	by	by	IN
cord-300796-rmjv56ia	68	27	a	a	DT
cord-300796-rmjv56ia	68	28	single	single	JJ
cord-300796-rmjv56ia	68	29	wash	wash	NN
cord-300796-rmjv56ia	68	30	with	with	IN
cord-300796-rmjv56ia	68	31	a	a	DT
cord-300796-rmjv56ia	68	32	buffer	buffer	NN
cord-300796-rmjv56ia	68	33	containing	contain	VBG
cord-300796-rmjv56ia	68	34	150	150	CD
cord-300796-rmjv56ia	68	35	mM	mm	CD
cord-300796-rmjv56ia	68	36	NaCI	naci	NN
cord-300796-rmjv56ia	68	37	,	,	,
cord-300796-rmjv56ia	68	38	10	10	CD
cord-300796-rmjv56ia	68	39	mM	mM	NNP
cord-300796-rmjv56ia	68	40	Tris	Tris	NNP
cord-300796-rmjv56ia	68	41	-	-	HYPH
cord-300796-rmjv56ia	68	42	HCl	HCl	NNP
cord-300796-rmjv56ia	68	43	pH	pH	NNP
cord-300796-rmjv56ia	68	44	7.4	7.4	CD
cord-300796-rmjv56ia	68	45	,	,	,
cord-300796-rmjv56ia	68	46	and	and	CC
cord-300796-rmjv56ia	68	47	20	20	CD
cord-300796-rmjv56ia	68	48	t	t	NN
cord-300796-rmjv56ia	68	49	~	~	NFP
cord-300796-rmjv56ia	68	50	g	g	NNP
cord-300796-rmjv56ia	68	51	PMSF	PMSF	NNP
cord-300796-rmjv56ia	68	52	/	/	SYM
cord-300796-rmjv56ia	68	53	ml	ml	NN
cord-300796-rmjv56ia	68	54	.	.	.
cord-300796-rmjv56ia	69	1	The	the	DT
cord-300796-rmjv56ia	69	2	beads	bead	NNS
cord-300796-rmjv56ia	69	3	were	be	VBD
cord-300796-rmjv56ia	69	4	then	then	RB
cord-300796-rmjv56ia	69	5	taken	take	VBN
cord-300796-rmjv56ia	69	6	up	up	RP
cord-300796-rmjv56ia	69	7	in	in	IN
cord-300796-rmjv56ia	69	8	excess	excess	JJ
cord-300796-rmjv56ia	69	9	gel	gel	NN
cord-300796-rmjv56ia	69	10	loading	loading	NN
cord-300796-rmjv56ia	69	11	buffer	buffer	NN
cord-300796-rmjv56ia	69	12	(	(	-LRB-
cord-300796-rmjv56ia	69	13	Cutler	Cutler	NNP
cord-300796-rmjv56ia	69	14	and	and	CC
cord-300796-rmjv56ia	69	15	Garoff	Garoff	NNP
cord-300796-rmjv56ia	69	16	,	,	,
cord-300796-rmjv56ia	69	17	1986	1986	CD
cord-300796-rmjv56ia	69	18	)	)	-RRB-
cord-300796-rmjv56ia	69	19	,	,	,
cord-300796-rmjv56ia	69	20	heated	heat	VBN
cord-300796-rmjv56ia	69	21	at	at	IN
cord-300796-rmjv56ia	69	22	95	95	CD
cord-300796-rmjv56ia	69	23	°	°	NN
cord-300796-rmjv56ia	69	24	C	c	NN
cord-300796-rmjv56ia	69	25	for	for	IN
cord-300796-rmjv56ia	69	26	5	5	CD
cord-300796-rmjv56ia	69	27	min	min	NN
cord-300796-rmjv56ia	69	28	,	,	,
cord-300796-rmjv56ia	69	29	and	and	CC
cord-300796-rmjv56ia	69	30	cleared	clear	VBN
cord-300796-rmjv56ia	69	31	by	by	IN
cord-300796-rmjv56ia	69	32	centrifugation	centrifugation	NN
cord-300796-rmjv56ia	69	33	before	before	IN
cord-300796-rmjv56ia	69	34	loading	load	VBG
cord-300796-rmjv56ia	69	35	the	the	DT
cord-300796-rmjv56ia	69	36	immunoprecipitate	immunoprecipitate	NN
cord-300796-rmjv56ia	69	37	on	on	IN
cord-300796-rmjv56ia	69	38	the	the	DT
cord-300796-rmjv56ia	69	39	gel	gel	NN
cord-300796-rmjv56ia	69	40	.	.	.
cord-300796-rmjv56ia	70	1	Constructions	construction	NNS
cord-300796-rmjv56ia	70	2	of	of	IN
cord-300796-rmjv56ia	70	3	pGEM2alphaGX	pGEM2alphaGX	NNP
cord-300796-rmjv56ia	70	4	and	and	CC
cord-300796-rmjv56ia	70	5	pGEM2dhfrX.	pGEM2dhfrX.	NNP
cord-300796-rmjv56ia	71	1	For	for	IN
cord-300796-rmjv56ia	71	2	the	the	DT
cord-300796-rmjv56ia	71	3	construction	construction	NN
cord-300796-rmjv56ia	71	4	of	of	IN
cord-300796-rmjv56ia	71	5	the	the	DT
cord-300796-rmjv56ia	71	6	final	final	JJ
cord-300796-rmjv56ia	71	7	fusion	fusion	NN
cord-300796-rmjv56ia	71	8	protein	protein	NN
cord-300796-rmjv56ia	71	9	-	-	HYPH
cord-300796-rmjv56ia	71	10	coding	code	VBG
cord-300796-rmjv56ia	71	11	plasmids	plasmid	NNS
cord-300796-rmjv56ia	71	12	used	use	VBN
cord-300796-rmjv56ia	71	13	in	in	IN
cord-300796-rmjv56ia	71	14	this	this	DT
cord-300796-rmjv56ia	71	15	study	study	NN
cord-300796-rmjv56ia	71	16	we	-PRON-	PRP
cord-300796-rmjv56ia	71	17	first	first	RB
cord-300796-rmjv56ia	71	18	had	have	VBD
cord-300796-rmjv56ia	71	19	to	to	TO
cord-300796-rmjv56ia	71	20	make	make	VB
cord-300796-rmjv56ia	71	21	plasmids	plasmid	NNS
cord-300796-rmjv56ia	71	22	pGEM2alphaGX	pGEM2alphaGX	NNP
cord-300796-rmjv56ia	71	23	,	,	,
cord-300796-rmjv56ia	71	24	which	which	WDT
cord-300796-rmjv56ia	71	25	are	be	VBP
cord-300796-rmjv56ia	71	26	derived	derive	VBN
cord-300796-rmjv56ia	71	27	from	from	IN
cord-300796-rmjv56ia	71	28	pGEM2alphaG	pGEM2alphaG	NNP
cord-300796-rmjv56ia	71	29	and	and	CC
cord-300796-rmjv56ia	71	30	pGEM2dhfrX	pGEM2dhfrX	NNP
cord-300796-rmjv56ia	71	31	,	,	,
cord-300796-rmjv56ia	71	32	which	which	WDT
cord-300796-rmjv56ia	71	33	are	be	VBP
cord-300796-rmjv56ia	71	34	derived	derive	VBN
cord-300796-rmjv56ia	71	35	from	from	IN
cord-300796-rmjv56ia	71	36	pGEM2dhfr	pgem2dhfr	PRP
cord-300796-rmjv56ia	71	37	(	(	-LRB-
cord-300796-rmjv56ia	71	38	Zerial	Zerial	NNP
cord-300796-rmjv56ia	71	39	et	et	FW
cord-300796-rmjv56ia	71	40	al	al	NNP
cord-300796-rmjv56ia	71	41	.	.	NNP
cord-300796-rmjv56ia	71	42	,	,	,
cord-300796-rmjv56ia	71	43	1986	1986	CD
cord-300796-rmjv56ia	71	44	)	)	-RRB-
cord-300796-rmjv56ia	71	45	.	.	.
cord-300796-rmjv56ia	72	1	Plasmid	Plasmid	NNP
cord-300796-rmjv56ia	72	2	pGEM2alphaG	pGEM2alphaG	NNP
cord-300796-rmjv56ia	72	3	contains	contain	VBZ
cord-300796-rmjv56ia	72	4	a	a	DT
cord-300796-rmjv56ia	72	5	548	548	CD
cord-300796-rmjv56ia	72	6	bp	bp	NNP
cord-300796-rmjv56ia	72	7	-	-	HYPH
cord-300796-rmjv56ia	72	8	long	long	JJ
cord-300796-rmjv56ia	72	9	Nco	Nco	NNP
cord-300796-rmjv56ia	72	10	I	I	NNP
cord-300796-rmjv56ia	72	11	-	-	HYPH
cord-300796-rmjv56ia	72	12	Pst	Pst	NNP
cord-300796-rmjv56ia	73	1	I	-PRON-	PRP
cord-300796-rmjv56ia	73	2	fragment	fragment	VBP
cord-300796-rmjv56ia	73	3	encompassing	encompass	VBG
cord-300796-rmjv56ia	73	4	the	the	DT
cord-300796-rmjv56ia	73	5	entire	entire	JJ
cord-300796-rmjv56ia	73	6	chimpanzee	chimpanzee	NN
cord-300796-rmjv56ia	73	7	alpha	alpha	NN
cord-300796-rmjv56ia	73	8	-	-	HYPH
cord-300796-rmjv56ia	73	9	globin	globin	NN
cord-300796-rmjv56ia	73	10	coding	code	VBG
cord-300796-rmjv56ia	73	11	region	region	NN
cord-300796-rmjv56ia	73	12	between	between	IN
cord-300796-rmjv56ia	73	13	the	the	DT
cord-300796-rmjv56ia	73	14	Hinc	Hinc	NNP
cord-300796-rmjv56ia	73	15	II	II	NNP
cord-300796-rmjv56ia	73	16	and	and	CC
cord-300796-rmjv56ia	74	1	Pst	Pst	NNP
cord-300796-rmjv56ia	74	2	I	i	CD
cord-300796-rmjv56ia	74	3	sites	site	NNS
cord-300796-rmjv56ia	74	4	of	of	IN
cord-300796-rmjv56ia	74	5	the	the	DT
cord-300796-rmjv56ia	74	6	polylinker	polylinker	NN
cord-300796-rmjv56ia	74	7	of	of	IN
cord-300796-rmjv56ia	74	8	the	the	DT
cord-300796-rmjv56ia	74	9	plasmid	plasmid	NN
cord-300796-rmjv56ia	74	10	pGEM1	pgem1	XX
cord-300796-rmjv56ia	75	1	(	(	-LRB-
cord-300796-rmjv56ia	75	2	Pmmega	Pmmega	NNP
cord-300796-rmjv56ia	75	3	Biotech	Biotech	NNP
cord-300796-rmjv56ia	75	4	)	)	-RRB-
cord-300796-rmjv56ia	75	5	.	.	.
cord-300796-rmjv56ia	76	1	The	the	DT
cord-300796-rmjv56ia	76	2	Nco	Nco	NNP
cord-300796-rmjv56ia	76	3	I	-PRON-	PRP
cord-300796-rmjv56ia	76	4	site	site	NN
cord-300796-rmjv56ia	76	5	contains	contain	VBZ
cord-300796-rmjv56ia	76	6	the	the	DT
cord-300796-rmjv56ia	76	7	translation	translation	NN
cord-300796-rmjv56ia	76	8	initiation	initiation	NN
cord-300796-rmjv56ia	76	9	codon	codon	NN
cord-300796-rmjv56ia	76	10	from	from	IN
cord-300796-rmjv56ia	76	11	alpha	alpha	NN
cord-300796-rmjv56ia	76	12	-	-	HYPH
cord-300796-rmjv56ia	76	13	globin	globin	NN
cord-300796-rmjv56ia	77	1	(	(	-LRB-
cord-300796-rmjv56ia	77	2	Zerial	Zerial	NNP
cord-300796-rmjv56ia	77	3	et	et	FW
cord-300796-rmjv56ia	77	4	al	al	NNP
cord-300796-rmjv56ia	77	5	.	.	NNP
cord-300796-rmjv56ia	77	6	,	,	,
cord-300796-rmjv56ia	77	7	1986	1986	CD
cord-300796-rmjv56ia	77	8	)	)	-RRB-
cord-300796-rmjv56ia	77	9	.	.	.
cord-300796-rmjv56ia	78	1	An	an	DT
cord-300796-rmjv56ia	78	2	Xho	Xho	NNP
cord-300796-rmjv56ia	78	3	I	-PRON-	PRP
cord-300796-rmjv56ia	78	4	site	site	NN
cord-300796-rmjv56ia	78	5	,	,	,
cord-300796-rmjv56ia	78	6	allowing	allow	VBG
cord-300796-rmjv56ia	78	7	subsequent	subsequent	JJ
cord-300796-rmjv56ia	78	8	in	in	IN
cord-300796-rmjv56ia	78	9	-	-	HYPH
cord-300796-rmjv56ia	78	10	frame	frame	NN
cord-300796-rmjv56ia	78	11	ligations	ligation	NNS
cord-300796-rmjv56ia	78	12	of	of	IN
cord-300796-rmjv56ia	78	13	SFV	sfv	NN
cord-300796-rmjv56ia	78	14	sequences	sequence	NNS
cord-300796-rmjv56ia	78	15	,	,	,
cord-300796-rmjv56ia	78	16	had	have	VBD
cord-300796-rmjv56ia	78	17	to	to	TO
cord-300796-rmjv56ia	78	18	be	be	VB
cord-300796-rmjv56ia	78	19	introduced	introduce	VBN
cord-300796-rmjv56ia	78	20	in	in	IN
cord-300796-rmjv56ia	78	21	pGEM2alphaG.	pgem2alphag.	NN
cord-300796-rmjv56ia	78	22	Therefore	therefore	RB
cord-300796-rmjv56ia	78	23	,	,	,
cord-300796-rmjv56ia	78	24	this	this	DT
cord-300796-rmjv56ia	78	25	plasmid	plasmid	NN
cord-300796-rmjv56ia	78	26	was	be	VBD
cord-300796-rmjv56ia	78	27	cut	cut	VBN
cord-300796-rmjv56ia	78	28	(	(	-LRB-
cord-300796-rmjv56ia	78	29	upstream	upstream	RB
cord-300796-rmjv56ia	78	30	of	of	IN
cord-300796-rmjv56ia	78	31	the	the	DT
cord-300796-rmjv56ia	78	32	Nco	Nco	NNP
cord-300796-rmjv56ia	78	33	I	I	NNP
cord-300796-rmjv56ia	78	34	site	site	NN
cord-300796-rmjv56ia	78	35	)	)	-RRB-
cord-300796-rmjv56ia	78	36	with	with	IN
cord-300796-rmjv56ia	78	37	Sac	Sac	NNP
cord-300796-rmjv56ia	78	38	I	I	NNP
cord-300796-rmjv56ia	78	39	,	,	,
cord-300796-rmjv56ia	78	40	the	the	DT
cord-300796-rmjv56ia	78	41	3	3	CD
cord-300796-rmjv56ia	78	42	'	'	``
cord-300796-rmjv56ia	78	43	sticky	sticky	JJ
cord-300796-rmjv56ia	78	44	ends	end	NNS
cord-300796-rmjv56ia	78	45	removed	remove	VBN
cord-300796-rmjv56ia	78	46	with	with	IN
cord-300796-rmjv56ia	78	47	"	"	``
cord-300796-rmjv56ia	78	48	1"4	1"4	CD
cord-300796-rmjv56ia	78	49	DNA	DNA	NNP
cord-300796-rmjv56ia	78	50	Folymerase	Folymerase	NNP
cord-300796-rmjv56ia	78	51	,	,	,
cord-300796-rmjv56ia	78	52	an	an	DT
cord-300796-rmjv56ia	78	53	Xho	Xho	NNP
cord-300796-rmjv56ia	79	1	I	-PRON-	PRP
cord-300796-rmjv56ia	79	2	octamer	octamer	VBP
cord-300796-rmjv56ia	79	3	linker	linker	NNP
cord-300796-rmjv56ia	79	4	introduced	introduce	VBD
cord-300796-rmjv56ia	79	5	and	and	CC
cord-300796-rmjv56ia	79	6	,	,	,
cord-300796-rmjv56ia	79	7	after	after	IN
cord-300796-rmjv56ia	79	8	cutting	cut	VBG
cord-300796-rmjv56ia	79	9	with	with	IN
cord-300796-rmjv56ia	79	10	Xho	Xho	NNP
cord-300796-rmjv56ia	80	1	I	-PRON-	PRP
cord-300796-rmjv56ia	80	2	,	,	,
cord-300796-rmjv56ia	80	3	the	the	DT
cord-300796-rmjv56ia	80	4	plasmid	plasmid	NN
cord-300796-rmjv56ia	80	5	was	be	VBD
cord-300796-rmjv56ia	80	6	religated	religate	VBN
cord-300796-rmjv56ia	80	7	at	at	IN
cord-300796-rmjv56ia	80	8	low	low	JJ
cord-300796-rmjv56ia	80	9	DNA	dna	NN
cord-300796-rmjv56ia	80	10	concentration	concentration	NN
cord-300796-rmjv56ia	80	11	(	(	-LRB-
cord-300796-rmjv56ia	80	12	1	1	CD
cord-300796-rmjv56ia	80	13	#	#	$
cord-300796-rmjv56ia	80	14	g	g	NN
cord-300796-rmjv56ia	80	15	/	/	SYM
cord-300796-rmjv56ia	80	16	ml	ml	NN
cord-300796-rmjv56ia	80	17	)	)	-RRB-
cord-300796-rmjv56ia	80	18	.	.	.
cord-300796-rmjv56ia	81	1	Plasmid	Plasmid	NNP
cord-300796-rmjv56ia	81	2	pGEM2alphaGX	pGEM2alphaGX	NNP
cord-300796-rmjv56ia	81	3	then	then	RB
cord-300796-rmjv56ia	81	4	contains	contain	VBZ
cord-300796-rmjv56ia	81	5	the	the	DT
cord-300796-rmjv56ia	81	6	2,057	2,057	CD
cord-300796-rmjv56ia	81	7	bp	bp	NNP
cord-300796-rmjv56ia	81	8	-	-	NN
cord-300796-rmjv56ia	81	9	long	long	JJ
cord-300796-rmjv56ia	82	1	Xbo	Xbo	NNP
cord-300796-rmjv56ia	83	1	I	-PRON-	PRP
cord-300796-rmjv56ia	83	2	-	-	HYPH
cord-300796-rmjv56ia	83	3	Pvu	Pvu	NNP
cord-300796-rmjv56ia	84	1	I	-PRON-	PRP
cord-300796-rmjv56ia	84	2	fragment	fragment	VBP
cord-300796-rmjv56ia	84	3	needed	need	VBN
cord-300796-rmjv56ia	84	4	for	for	IN
cord-300796-rmjv56ia	84	5	the	the	DT
cord-300796-rmjv56ia	84	6	construction	construction	NN
cord-300796-rmjv56ia	84	7	of	of	IN
cord-300796-rmjv56ia	84	8	the	the	DT
cord-300796-rmjv56ia	84	9	fusion	fusion	NN
cord-300796-rmjv56ia	84	10	protein	protein	NN
cord-300796-rmjv56ia	84	11	-	-	HYPH
cord-300796-rmjv56ia	84	12	coding	code	VBG
cord-300796-rmjv56ia	84	13	plasmid	plasmid	NN
cord-300796-rmjv56ia	84	14	pC62alphaG.	pc62alphag.	XX
cord-300796-rmjv56ia	85	1	An	an	DT
cord-300796-rmjv56ia	85	2	intermediate	intermediate	JJ
cord-300796-rmjv56ia	85	3	construct	construct	NN
cord-300796-rmjv56ia	85	4	,	,	,
cord-300796-rmjv56ia	85	5	analogous	analogous	JJ
cord-300796-rmjv56ia	85	6	to	to	IN
cord-300796-rmjv56ia	85	7	pGEM2alphaGX	pGEM2alphaGX	NNP
cord-300796-rmjv56ia	85	8	,	,	,
cord-300796-rmjv56ia	85	9	and	and	CC
cord-300796-rmjv56ia	85	10	also	also	RB
cord-300796-rmjv56ia	85	11	conraining	conraine	VBG
cord-300796-rmjv56ia	85	12	a	a	DT
cord-300796-rmjv56ia	85	13	unique	unique	JJ
cord-300796-rmjv56ia	85	14	Xho	Xho	NNP
cord-300796-rmjv56ia	85	15	I	-PRON-	PRP
cord-300796-rmjv56ia	85	16	site	site	NN
cord-300796-rmjv56ia	85	17	,	,	,
cord-300796-rmjv56ia	85	18	was	be	VBD
cord-300796-rmjv56ia	85	19	needed	need	VBN
cord-300796-rmjv56ia	85	20	for	for	IN
cord-300796-rmjv56ia	85	21	the	the	DT
cord-300796-rmjv56ia	85	22	constructions	construction	NNS
cord-300796-rmjv56ia	85	23	of	of	IN
cord-300796-rmjv56ia	85	24	dhfrcontaining	dhfrcontaine	VBG
cord-300796-rmjv56ia	85	25	plasmids	plasmid	NNS
cord-300796-rmjv56ia	85	26	.	.	.
cord-300796-rmjv56ia	86	1	For	for	IN
cord-300796-rmjv56ia	86	2	this	this	DT
cord-300796-rmjv56ia	86	3	purpose	purpose	NN
cord-300796-rmjv56ia	86	4	we	-PRON-	PRP
cord-300796-rmjv56ia	86	5	inserted	insert	VBD
cord-300796-rmjv56ia	86	6	the	the	DT
cord-300796-rmjv56ia	86	7	Xho	Xho	NNP
cord-300796-rmjv56ia	86	8	I	-PRON-	PRP
cord-300796-rmjv56ia	86	9	linker	linker	VBP
cord-300796-rmjv56ia	86	10	into	into	IN
cord-300796-rmjv56ia	86	11	partially	partially	RB
cord-300796-rmjv56ia	86	12	Xmn	xmn	NN
cord-300796-rmjv56ia	87	1	I	-PRON-	PRP
cord-300796-rmjv56ia	87	2	cut	cut	VBD
cord-300796-rmjv56ia	87	3	pGEM2dhfr	pGEM2dhfr	NNS
cord-300796-rmjv56ia	87	4	(	(	-LRB-
cord-300796-rmjv56ia	87	5	Zerial	Zerial	NNP
cord-300796-rmjv56ia	87	6	et	et	FW
cord-300796-rmjv56ia	87	7	al	al	NNP
cord-300796-rmjv56ia	87	8	.	.	NNP
cord-300796-rmjv56ia	87	9	,	,	,
cord-300796-rmjv56ia	87	10	1986	1986	CD
cord-300796-rmjv56ia	87	11	)	)	-RRB-
cord-300796-rmjv56ia	87	12	.	.	.
cord-300796-rmjv56ia	88	1	After	after	IN
cord-300796-rmjv56ia	88	2	cutting	cut	VBG
cord-300796-rmjv56ia	88	3	the	the	DT
cord-300796-rmjv56ia	88	4	linkers	linker	NNS
cord-300796-rmjv56ia	88	5	,	,	,
cord-300796-rmjv56ia	88	6	linear	linear	JJ
cord-300796-rmjv56ia	88	7	plasmid	plasmid	NN
cord-300796-rmjv56ia	88	8	was	be	VBD
cord-300796-rmjv56ia	88	9	purified	purify	VBN
cord-300796-rmjv56ia	88	10	on	on	IN
cord-300796-rmjv56ia	88	11	agarose	agarose	JJ
cord-300796-rmjv56ia	88	12	gel	gel	NN
cord-300796-rmjv56ia	88	13	and	and	CC
cord-300796-rmjv56ia	88	14	religated	religate	VBN
cord-300796-rmjv56ia	88	15	.	.	.
cord-300796-rmjv56ia	89	1	Since	since	IN
cord-300796-rmjv56ia	89	2	the	the	DT
cord-300796-rmjv56ia	89	3	second	second	JJ
cord-300796-rmjv56ia	89	4	Xmn	Xmn	NNP
cord-300796-rmjv56ia	89	5	I	-PRON-	PRP
cord-300796-rmjv56ia	89	6	site	site	VBP
cord-300796-rmjv56ia	89	7	in	in	IN
cord-300796-rmjv56ia	89	8	pGEM2dhfr	pGEM2dhfr	NNP
cord-300796-rmjv56ia	89	9	is	be	VBZ
cord-300796-rmjv56ia	89	10	located	locate	VBN
cord-300796-rmjv56ia	89	11	in	in	IN
cord-300796-rmjv56ia	89	12	the	the	DT
cord-300796-rmjv56ia	89	13	beta	beta	NN
cord-300796-rmjv56ia	89	14	-	-	HYPH
cord-300796-rmjv56ia	89	15	lactamase	lactamase	NN
cord-300796-rmjv56ia	89	16	coding	coding	NN
cord-300796-rmjv56ia	89	17	region	region	NN
cord-300796-rmjv56ia	89	18	of	of	IN
cord-300796-rmjv56ia	89	19	the	the	DT
cord-300796-rmjv56ia	89	20	vector	vector	NN
cord-300796-rmjv56ia	90	1	(	(	-LRB-
cord-300796-rmjv56ia	90	2	Snt	Snt	NNP
cord-300796-rmjv56ia	90	3	~	~	NNP
cord-300796-rmjv56ia	90	4	liffe	liffe	NN
cord-300796-rmjv56ia	90	5	,	,	,
cord-300796-rmjv56ia	90	6	1979	1979	CD
cord-300796-rmjv56ia	90	7	)	)	-RRB-
cord-300796-rmjv56ia	90	8	and	and	CC
cord-300796-rmjv56ia	90	9	insertion	insertion	NN
cord-300796-rmjv56ia	90	10	of	of	IN
cord-300796-rmjv56ia	90	11	an	an	DT
cord-300796-rmjv56ia	90	12	Xho	Xho	NNP
cord-300796-rmjv56ia	91	1	I	-PRON-	PRP
cord-300796-rmjv56ia	91	2	site	site	VBP
cord-300796-rmjv56ia	91	3	by	by	IN
cord-300796-rmjv56ia	91	4	an	an	DT
cord-300796-rmjv56ia	91	5	octamer	octamer	NN
cord-300796-rmjv56ia	91	6	linker	linker	NN
cord-300796-rmjv56ia	91	7	will	will	MD
cord-300796-rmjv56ia	91	8	result	result	VB
cord-300796-rmjv56ia	91	9	in	in	IN
cord-300796-rmjv56ia	91	10	an	an	DT
cord-300796-rmjv56ia	91	11	ampicillin	ampicillin	NN
cord-300796-rmjv56ia	91	12	-	-	HYPH
cord-300796-rmjv56ia	91	13	sensitive	sensitive	JJ
cord-300796-rmjv56ia	91	14	E.	E.	NNP
cord-300796-rmjv56ia	91	15	coli	coli	NNS
cord-300796-rmjv56ia	91	16	phenotype	phenotype	NN
cord-300796-rmjv56ia	91	17	after	after	IN
cord-300796-rmjv56ia	91	18	transformation	transformation	NN
cord-300796-rmjv56ia	91	19	,	,	,
cord-300796-rmjv56ia	91	20	only	only	RB
cord-300796-rmjv56ia	91	21	the	the	DT
cord-300796-rmjv56ia	91	22	desired	desire	VBN
cord-300796-rmjv56ia	91	23	pGEM2dhfrX	pgem2dhfrx	JJ
cord-300796-rmjv56ia	91	24	construct	construct	NN
cord-300796-rmjv56ia	91	25	was	be	VBD
cord-300796-rmjv56ia	91	26	obtained	obtain	VBN
cord-300796-rmjv56ia	91	27	.	.	.
cord-300796-rmjv56ia	92	1	From	from	IN
cord-300796-rmjv56ia	92	2	this	this	DT
cord-300796-rmjv56ia	92	3	plasmid	plasmid	NN
cord-300796-rmjv56ia	92	4	,	,	,
cord-300796-rmjv56ia	92	5	an	an	DT
cord-300796-rmjv56ia	92	6	Xho	Xho	NNP
cord-300796-rmjv56ia	92	7	I	I	NNP
cord-300796-rmjv56ia	92	8	-	-	HYPH
cord-300796-rmjv56ia	92	9	Pvu	Pvu	NNP
cord-300796-rmjv56ia	92	10	'	'	''
cord-300796-rmjv56ia	92	11	I	-PRON-	PRP
cord-300796-rmjv56ia	92	12	fragment	fragment	VBP
cord-300796-rmjv56ia	92	13	of	of	IN
cord-300796-rmjv56ia	92	14	at	at	RB
cord-300796-rmjv56ia	92	15	least	least	RBS
cord-300796-rmjv56ia	92	16	2,012	2,012	CD
cord-300796-rmjv56ia	92	17	bp	bp	NNP
cord-300796-rmjv56ia	92	18	(	(	-LRB-
cord-300796-rmjv56ia	92	19	the	the	DT
cord-300796-rmjv56ia	92	20	precise	precise	JJ
cord-300796-rmjv56ia	92	21	length	length	NN
cord-300796-rmjv56ia	92	22	of	of	IN
cord-300796-rmjv56ia	92	23	the	the	DT
cord-300796-rmjv56ia	92	24	cDNA	cdna	NN
cord-300796-rmjv56ia	92	25	insert	insert	NN
cord-300796-rmjv56ia	92	26	,	,	,
cord-300796-rmjv56ia	92	27	i.e.	i.e.	FW
cord-300796-rmjv56ia	92	28	,	,	,
cord-300796-rmjv56ia	92	29	the	the	DT
cord-300796-rmjv56ia	92	30	length	length	NN
cord-300796-rmjv56ia	92	31	of	of	IN
cord-300796-rmjv56ia	92	32	the	the	DT
cord-300796-rmjv56ia	92	33	3	3	CD
cord-300796-rmjv56ia	92	34	'	'	POS
cord-300796-rmjv56ia	92	35	untranslated	untranslated	JJ
cord-300796-rmjv56ia	92	36	region	region	NN
cord-300796-rmjv56ia	92	37	of	of	IN
cord-300796-rmjv56ia	92	38	dhfr	dhfr	NN
cord-300796-rmjv56ia	92	39	,	,	,
cord-300796-rmjv56ia	92	40	is	be	VBZ
cord-300796-rmjv56ia	92	41	not	not	RB
cord-300796-rmjv56ia	92	42	known	know	VBN
cord-300796-rmjv56ia	92	43	in	in	IN
cord-300796-rmjv56ia	92	44	pGEM2dhfr	pGEM2dhfr	NNS
cord-300796-rmjv56ia	92	45	)	)	-RRB-
cord-300796-rmjv56ia	92	46	was	be	VBD
cord-300796-rmjv56ia	92	47	used	use	VBN
cord-300796-rmjv56ia	92	48	for	for	IN
cord-300796-rmjv56ia	92	49	the	the	DT
cord-300796-rmjv56ia	92	50	construction	construction	NN
cord-300796-rmjv56ia	92	51	of	of	IN
cord-300796-rmjv56ia	92	52	pC62dhfr	pC62dhfr	NNP
cord-300796-rmjv56ia	92	53	.	.	.
cord-300796-rmjv56ia	93	1	Construction	construction	NN
cord-300796-rmjv56ia	93	2	of	of	IN
cord-300796-rmjv56ia	93	3	the	the	DT
cord-300796-rmjv56ia	93	4	Fusion	Fusion	NNP
cord-300796-rmjv56ia	93	5	Protein	Protein	NNP
cord-300796-rmjv56ia	93	6	-	-	HYPH
cord-300796-rmjv56ia	93	7	coding	code	VBG
cord-300796-rmjv56ia	93	8	Plasmids	Plasmids	NNP
cord-300796-rmjv56ia	93	9	pC62alphaG	pC62alphaG	NNP
cord-300796-rmjv56ia	93	10	and	and	CC
cord-300796-rmjv56ia	93	11	pC62dhfr	pc62dhfr	NN
cord-300796-rmjv56ia	93	12	.	.	.
cord-300796-rmjv56ia	94	1	Plasmid	Plasmid	NNP
cord-300796-rmjv56ia	94	2	pGEMI	pgemi	NN
cord-300796-rmjv56ia	94	3	-	-	HYPH
cord-300796-rmjv56ia	94	4	SFV	sfv	NN
cord-300796-rmjv56ia	94	5	(	(	-LRB-
cord-300796-rmjv56ia	94	6	also	also	RB
cord-300796-rmjv56ia	94	7	called	call	VBN
cord-300796-rmjv56ia	94	8	pG	pg	NN
cord-300796-rmjv56ia	94	9	-	-	NN
cord-300796-rmjv56ia	94	10	SFV-15/5	sfv-15/5	VB
cord-300796-rmjv56ia	94	11	;	;	:
cord-300796-rmjv56ia	94	12	Melancon	Melancon	NNP
cord-300796-rmjv56ia	94	13	and	and	CC
cord-300796-rmjv56ia	94	14	Garoff	Garoff	NNP
cord-300796-rmjv56ia	94	15	,	,	,
cord-300796-rmjv56ia	94	16	1986	1986	CD
cord-300796-rmjv56ia	94	17	)	)	-RRB-
cord-300796-rmjv56ia	94	18	contains	contain	VBZ
cord-300796-rmjv56ia	94	19	a	a	DT
cord-300796-rmjv56ia	94	20	re	re	NN
cord-300796-rmjv56ia	94	21	,	,	,
cord-300796-rmjv56ia	94	22	engineered	engineer	VBN
cord-300796-rmjv56ia	94	23	eDNA	edna	JJ
cord-300796-rmjv56ia	94	24	copy	copy	NN
cord-300796-rmjv56ia	94	25	of	of	IN
cord-300796-rmjv56ia	94	26	the	the	DT
cord-300796-rmjv56ia	94	27	SFV	SFV	NNP
cord-300796-rmjv56ia	94	28	26S	26S	NNP
cord-300796-rmjv56ia	94	29	mRNA	mrna	NN
cord-300796-rmjv56ia	94	30	sequences	sequence	NNS
cord-300796-rmjv56ia	94	31	cloned	clone	VBN
cord-300796-rmjv56ia	94	32	as	as	IN
cord-300796-rmjv56ia	94	33	a	a	DT
cord-300796-rmjv56ia	94	34	Barn	Barn	NNP
cord-300796-rmjv56ia	94	35	HI	HI	NNP
cord-300796-rmjv56ia	94	36	fragment	fragment	NN
cord-300796-rmjv56ia	94	37	in	in	IN
cord-300796-rmjv56ia	94	38	the	the	DT
cord-300796-rmjv56ia	94	39	Barn	Barn	NNP
cord-300796-rmjv56ia	94	40	HI	HI	NNP
cord-300796-rmjv56ia	94	41	site	site	NN
cord-300796-rmjv56ia	94	42	of	of	IN
cord-300796-rmjv56ia	94	43	the	the	DT
cord-300796-rmjv56ia	94	44	polylinker	polylinker	JJ
cord-300796-rmjv56ia	94	45	downstream	downstream	NN
cord-300796-rmjv56ia	94	46	of	of	IN
cord-300796-rmjv56ia	94	47	the	the	DT
cord-300796-rmjv56ia	94	48	SP6	SP6	NNP
cord-300796-rmjv56ia	94	49	promoter	promoter	NN
cord-300796-rmjv56ia	94	50	in	in	IN
cord-300796-rmjv56ia	94	51	the	the	DT
cord-300796-rmjv56ia	94	52	plasmid	plasmid	NN
cord-300796-rmjv56ia	94	53	pGEM1	pgem1	XX
cord-300796-rmjv56ia	95	1	(	(	-LRB-
cord-300796-rmjv56ia	95	2	Promaga	Promaga	NNP
cord-300796-rmjv56ia	95	3	Biotech	Biotech	NNP
cord-300796-rmjv56ia	95	4	)	)	-RRB-
cord-300796-rmjv56ia	95	5	.	.	.
cord-300796-rmjv56ia	96	1	From	from	IN
cord-300796-rmjv56ia	96	2	the	the	DT
cord-300796-rmjv56ia	96	3	SFV	sfv	NN
cord-300796-rmjv56ia	96	4	plasmid	plasmid	NN
cord-300796-rmjv56ia	96	5	,	,	,
cord-300796-rmjv56ia	96	6	a	a	DT
cord-300796-rmjv56ia	96	7	2,381	2,381	CD
cord-300796-rmjv56ia	96	8	hp	hp	NN
cord-300796-rmjv56ia	96	9	-	-	JJ
cord-300796-rmjv56ia	96	10	long	long	JJ
cord-300796-rmjv56ia	96	11	Pvu	Pvu	NNP
cord-300796-rmjv56ia	96	12	I	I	NNP
cord-300796-rmjv56ia	96	13	-	-	HYPH
cord-300796-rmjv56ia	96	14	Xho	Xho	NNP
cord-300796-rmjv56ia	96	15	I	-PRON-	PRP
cord-300796-rmjv56ia	96	16	fragment	fragment	NN
cord-300796-rmjv56ia	96	17	,	,	,
cord-300796-rmjv56ia	96	18	containing	contain	VBG
cord-300796-rmjv56ia	96	19	the	the	DT
cord-300796-rmjv56ia	96	20	coding	code	VBG
cord-300796-rmjv56ia	96	21	sequences	sequence	NNS
cord-300796-rmjv56ia	96	22	for	for	IN
cord-300796-rmjv56ia	96	23	the	the	DT
cord-300796-rmjv56ia	96	24	capsid	capsid	JJ
cord-300796-rmjv56ia	96	25	protein	protein	NN
cord-300796-rmjv56ia	96	26	and	and	CC
cord-300796-rmjv56ia	96	27	the	the	DT
cord-300796-rmjv56ia	96	28	NH2-terminai	NH2-terminai	NNP
cord-300796-rmjv56ia	96	29	region	region	NN
cord-300796-rmjv56ia	96	30	of	of	IN
cord-300796-rmjv56ia	96	31	the	the	DT
cord-300796-rmjv56ia	96	32	p62	p62	NN
cord-300796-rmjv56ia	96	33	protein	protein	NN
cord-300796-rmjv56ia	96	34	,	,	,
cord-300796-rmjv56ia	96	35	was	be	VBD
cord-300796-rmjv56ia	96	36	isolated	isolate	VBN
cord-300796-rmjv56ia	96	37	.	.	.
cord-300796-rmjv56ia	97	1	The	the	DT
cord-300796-rmjv56ia	97	2	Xho	Xho	NNP
cord-300796-rmjv56ia	98	1	I	-PRON-	PRP
cord-300796-rmjv56ia	98	2	-	-	HYPH
cord-300796-rmjv56ia	98	3	Pvu	Pvu	NNP
cord-300796-rmjv56ia	99	1	I	-PRON-	PRP
cord-300796-rmjv56ia	99	2	fragments	fragment	VBZ
cord-300796-rmjv56ia	99	3	from	from	IN
cord-300796-rmjv56ia	99	4	pGEMI	pgemi	NN
cord-300796-rmjv56ia	99	5	-	-	HYPH
cord-300796-rmjv56ia	99	6	SFV	sfv	NN
cord-300796-rmjv56ia	99	7	,	,	,
cord-300796-rmjv56ia	99	8	pGEM2alphaGX	pGEM2alphaGX	NNP
cord-300796-rmjv56ia	99	9	and	and	CC
cord-300796-rmjv56ia	99	10	pGEM2dhfrX	pGEM2dhfrX	NNP
cord-300796-rmjv56ia	99	11	were	be	VBD
cord-300796-rmjv56ia	99	12	isolated	isolate	VBN
cord-300796-rmjv56ia	99	13	and	and	CC
cord-300796-rmjv56ia	99	14	ligated	ligate	VBN
cord-300796-rmjv56ia	99	15	at	at	IN
cord-300796-rmjv56ia	99	16	a	a	DT
cord-300796-rmjv56ia	99	17	1:1	1:1	CD
cord-300796-rmjv56ia	99	18	molar	molar	JJ
cord-300796-rmjv56ia	99	19	ratio	ratio	NN
cord-300796-rmjv56ia	99	20	to	to	TO
cord-300796-rmjv56ia	99	21	obtain	obtain	VB
cord-300796-rmjv56ia	99	22	pC62alphaG	pC62alphaG	NNP
cord-300796-rmjv56ia	99	23	and	and	CC
cord-300796-rmjv56ia	99	24	pC62dhfr	pC62dhfr	NNP
cord-300796-rmjv56ia	99	25	,	,	,
cord-300796-rmjv56ia	99	26	respectively	respectively	RB
cord-300796-rmjv56ia	99	27	.	.	.
cord-300796-rmjv56ia	100	1	Plasmid	Plasmid	NNP
cord-300796-rmjv56ia	100	2	DNAs	dna	NNS
cord-300796-rmjv56ia	100	3	from	from	IN
cord-300796-rmjv56ia	100	4	ampicillin	ampicillin	JJ
cord-300796-rmjv56ia	100	5	-	-	HYPH
cord-300796-rmjv56ia	100	6	resistant	resistant	JJ
cord-300796-rmjv56ia	100	7	colonies	colony	NNS
cord-300796-rmjv56ia	100	8	were	be	VBD
cord-300796-rmjv56ia	100	9	screened	screen	VBN
cord-300796-rmjv56ia	100	10	and	and	CC
cord-300796-rmjv56ia	100	11	compared	compare	VBN
cord-300796-rmjv56ia	100	12	to	to	IN
cord-300796-rmjv56ia	100	13	the	the	DT
cord-300796-rmjv56ia	100	14	starting	starting	NN
cord-300796-rmjv56ia	100	15	vectors	vector	NNS
cord-300796-rmjv56ia	100	16	by	by	IN
cord-300796-rmjv56ia	100	17	restriction	restriction	NN
cord-300796-rmjv56ia	100	18	analysis	analysis	NN
cord-300796-rmjv56ia	100	19	.	.	.
cord-300796-rmjv56ia	101	1	Altogether	altogether	RB
cord-300796-rmjv56ia	101	2	,	,	,
cord-300796-rmjv56ia	101	3	the	the	DT
cord-300796-rmjv56ia	101	4	SFV	sfv	NN
cord-300796-rmjv56ia	101	5	-	-	HYPH
cord-300796-rmjv56ia	101	6	alpha	alpha	NN
cord-300796-rmjv56ia	101	7	-	-	HYPH
cord-300796-rmjv56ia	101	8	globin	globin	NN
cord-300796-rmjv56ia	101	9	eDNA	edna	JJ
cord-300796-rmjv56ia	101	10	fusion	fusion	NN
cord-300796-rmjv56ia	101	11	results	result	NNS
cord-300796-rmjv56ia	101	12	in	in	IN
cord-300796-rmjv56ia	101	13	a	a	DT
cord-300796-rmjv56ia	101	14	complete	complete	JJ
cord-300796-rmjv56ia	101	15	C	c	NN
cord-300796-rmjv56ia	101	16	region	region	NN
cord-300796-rmjv56ia	101	17	and	and	CC
cord-300796-rmjv56ia	101	18	40	40	CD
cord-300796-rmjv56ia	101	19	codons	codon	NNS
cord-300796-rmjv56ia	101	20	from	from	IN
cord-300796-rmjv56ia	101	21	the	the	DT
cord-300796-rmjv56ia	101	22	5	5	CD
cord-300796-rmjv56ia	101	23	'	'	NN
cord-300796-rmjv56ia	101	24	end	end	NN
cord-300796-rmjv56ia	101	25	of	of	IN
cord-300796-rmjv56ia	101	26	the	the	DT
cord-300796-rmjv56ia	101	27	p62	p62	NN
cord-300796-rmjv56ia	101	28	region	region	NN
cord-300796-rmjv56ia	101	29	fused	fuse	VBN
cord-300796-rmjv56ia	101	30	to	to	IN
cord-300796-rmjv56ia	101	31	the	the	DT
cord-300796-rmjv56ia	101	32	whole	whole	NN
cord-300796-rmjv56ia	101	33	of	of	IN
cord-300796-rmjv56ia	101	34	the	the	DT
cord-300796-rmjv56ia	101	35	alpha	alpha	NN
cord-300796-rmjv56ia	101	36	-	-	HYPH
cord-300796-rmjv56ia	101	37	globin	globin	NN
cord-300796-rmjv56ia	101	38	coding	code	VBG
cord-300796-rmjv56ia	101	39	sequence	sequence	NN
cord-300796-rmjv56ia	101	40	(	(	-LRB-
cord-300796-rmjv56ia	101	41	see	see	VB
cord-300796-rmjv56ia	101	42	Fig	Fig	NNP
cord-300796-rmjv56ia	101	43	.	.	NNP
cord-300796-rmjv56ia	101	44	1	1	CD
cord-300796-rmjv56ia	101	45	)	)	-RRB-
cord-300796-rmjv56ia	101	46	.	.	.
cord-300796-rmjv56ia	102	1	Eight	eight	CD
cord-300796-rmjv56ia	102	2	new	new	JJ
cord-300796-rmjv56ia	102	3	codons	codon	NNS
cord-300796-rmjv56ia	102	4	have	have	VBP
cord-300796-rmjv56ia	102	5	been	be	VBN
cord-300796-rmjv56ia	102	6	introduced	introduce	VBN
cord-300796-rmjv56ia	102	7	at	at	IN
cord-300796-rmjv56ia	102	8	the	the	DT
cord-300796-rmjv56ia	102	9	point	point	NN
cord-300796-rmjv56ia	102	10	of	of	IN
cord-300796-rmjv56ia	102	11	eDNA	edna	JJ
cord-300796-rmjv56ia	102	12	fusion	fusion	NN
cord-300796-rmjv56ia	102	13	.	.	.
cord-300796-rmjv56ia	103	1	In	in	IN
cord-300796-rmjv56ia	103	2	the	the	DT
cord-300796-rmjv56ia	103	3	SFV	SFV	NNP
cord-300796-rmjv56ia	103	4	-	-	HYPH
cord-300796-rmjv56ia	103	5	dhfr	dhfr	NN
cord-300796-rmjv56ia	103	6	construction	construction	NN
cord-300796-rmjv56ia	103	7	the	the	DT
cord-300796-rmjv56ia	103	8	C	C	NNP
cord-300796-rmjv56ia	103	9	region	region	NN
cord-300796-rmjv56ia	103	10	and	and	CC
cord-300796-rmjv56ia	103	11	the	the	DT
cord-300796-rmjv56ia	103	12	40	40	CD
cord-300796-rmjv56ia	103	13	first	first	JJ
cord-300796-rmjv56ia	103	14	codons	codon	NNS
cord-300796-rmjv56ia	103	15	of	of	IN
cord-300796-rmjv56ia	103	16	p62	p62	NN
cord-300796-rmjv56ia	103	17	are	be	VBP
cord-300796-rmjv56ia	103	18	fused	fuse	VBN
cord-300796-rmjv56ia	103	19	to	to	IN
cord-300796-rmjv56ia	103	20	the	the	DT
cord-300796-rmjv56ia	103	21	dhfr	dhfr	NN
cord-300796-rmjv56ia	103	22	coding	code	VBG
cord-300796-rmjv56ia	103	23	sequence	sequence	NN
cord-300796-rmjv56ia	103	24	such	such	JJ
cord-300796-rmjv56ia	103	25	that	that	IN
cord-300796-rmjv56ia	103	26	one	one	CD
cord-300796-rmjv56ia	103	27	new	new	JJ
cord-300796-rmjv56ia	103	28	codon	codon	NN
cord-300796-rmjv56ia	103	29	is	be	VBZ
cord-300796-rmjv56ia	103	30	introduced	introduce	VBN
cord-300796-rmjv56ia	103	31	and	and	CC
cord-300796-rmjv56ia	103	32	the	the	DT
cord-300796-rmjv56ia	103	33	first	first	JJ
cord-300796-rmjv56ia	103	34	31	31	CD
cord-300796-rmjv56ia	103	35	codons	codon	NNS
cord-300796-rmjv56ia	103	36	of	of	IN
cord-300796-rmjv56ia	103	37	dhfr	dhfr	NNS
cord-300796-rmjv56ia	103	38	are	be	VBP
cord-300796-rmjv56ia	103	39	lost	lose	VBN
cord-300796-rmjv56ia	103	40	.	.	.
cord-300796-rmjv56ia	104	1	Construction	construction	NN
cord-300796-rmjv56ia	104	2	of	of	IN
cord-300796-rmjv56ia	104	3	Plasmidp62dhfr	Plasmidp62dhfr	NNP
cord-300796-rmjv56ia	104	4	.	.	.
cord-300796-rmjv56ia	105	1	For	for	IN
cord-300796-rmjv56ia	105	2	engineering	engineering	NN
cord-300796-rmjv56ia	105	3	of	of	IN
cord-300796-rmjv56ia	105	4	a	a	DT
cord-300796-rmjv56ia	105	5	p62	p62	NN
cord-300796-rmjv56ia	105	6	protein	protein	NN
cord-300796-rmjv56ia	105	7	signal	signal	NN
cord-300796-rmjv56ia	105	8	sequence	sequence	NN
cord-300796-rmjv56ia	105	9	-	-	HYPH
cord-300796-rmjv56ia	105	10	dhfr	dhfr	NN
cord-300796-rmjv56ia	105	11	fusion	fusion	NN
cord-300796-rmjv56ia	105	12	protein	protein	NN
cord-300796-rmjv56ia	105	13	which	which	WDT
cord-300796-rmjv56ia	105	14	is	be	VBZ
cord-300796-rmjv56ia	105	15	not	not	RB
cord-300796-rmjv56ia	105	16	derived	derive	VBN
cord-300796-rmjv56ia	105	17	from	from	IN
cord-300796-rmjv56ia	105	18	a	a	DT
cord-300796-rmjv56ia	105	19	C	C	NNP
cord-300796-rmjv56ia	105	20	proteincontaining	proteincontaining	NN
cord-300796-rmjv56ia	105	21	precursor	precursor	NN
cord-300796-rmjv56ia	105	22	we	-PRON-	PRP
cord-300796-rmjv56ia	105	23	synthesized	synthesize	VBD
cord-300796-rmjv56ia	105	24	the	the	DT
cord-300796-rmjv56ia	105	25	whole	whole	JJ
cord-300796-rmjv56ia	105	26	p62	p62	NN
cord-300796-rmjv56ia	105	27	signal	signal	NN
cord-300796-rmjv56ia	105	28	sequence	sequence	NN
cord-300796-rmjv56ia	105	29	region	region	NN
cord-300796-rmjv56ia	105	30	.	.	.
cord-300796-rmjv56ia	106	1	Two	two	CD
cord-300796-rmjv56ia	106	2	overlapping	overlap	VBG
cord-300796-rmjv56ia	106	3	oligonucleotides	oligonucleotide	NNS
cord-300796-rmjv56ia	106	4	were	be	VBD
cord-300796-rmjv56ia	106	5	made	make	VBN
cord-300796-rmjv56ia	106	6	(	(	-LRB-
cord-300796-rmjv56ia	106	7	DNA	dna	NN
cord-300796-rmjv56ia	106	8	-	-	HYPH
cord-300796-rmjv56ia	106	9	synthesizer	synthesizer	NN
cord-300796-rmjv56ia	106	10	;	;	:
cord-300796-rmjv56ia	106	11	Applied	Applied	NNP
cord-300796-rmjv56ia	106	12	Biosystems	Biosystems	NNP
cord-300796-rmjv56ia	106	13	,	,	,
cord-300796-rmjv56ia	106	14	Foster	Foster	NNP
cord-300796-rmjv56ia	106	15	City	City	NNP
cord-300796-rmjv56ia	106	16	,	,	,
cord-300796-rmjv56ia	106	17	CA	CA	NNP
cord-300796-rmjv56ia	106	18	)	)	-RRB-
cord-300796-rmjv56ia	106	19	:	:	:
cord-300796-rmjv56ia	106	20	(	(	-LRB-
cord-300796-rmjv56ia	106	21	1	1	LS
cord-300796-rmjv56ia	106	22	)	)	-RRB-
cord-300796-rmjv56ia	106	23	5	5	CD
cord-300796-rmjv56ia	106	24	'	'	POS
cord-300796-rmjv56ia	106	25	ATACACAGAATTCAGCACCATGT	ATACACAGAATTCAGCACCATGT	NNP
cord-300796-rmjv56ia	106	26	-	-	HYPH
cord-300796-rmjv56ia	106	27	CCGCCCCGCTGATTAC	CCGCCCCGCTGATTAC	NNP
cord-300796-rmjv56ia	106	28	TGCCATGTGTGTCCTIV	TGCCATGTGTGTCCTIV	NNP
cord-300796-rmjv56ia	107	1	~CAATC_~TACCT	~CAATC_~TACCT	NNP
cord-300796-rmjv56ia	107	2	-	-	HYPH
cord-300796-rmjv56ia	107	3	TCCCGTC~	TCCCGTC~	NNP
cord-300796-rmjv56ia	108	1	TTCCAGCCCCCGTGTGTACC~	TTCCAGCCCCCGTGTGTACC~	NNP
cord-300796-rmjv56ia	108	2	,	,	,
cord-300796-rmjv56ia	108	3	(	(	-LRB-
cord-300796-rmjv56ia	108	4	2	2	LS
cord-300796-rmjv56ia	108	5	)	)	-RRB-
cord-300796-rmjv56ia	108	6	5	5	CD
cord-300796-rmjv56ia	108	7	'	'	''
cord-300796-rmjv56ia	109	1	GTTATCCT	GTTATCCT	NNP
cord-300796-rmjv56ia	109	2	-	-	HYPH
cord-300796-rmjv56ia	109	3	CGAGCATCCGTAGTGTGGCCTCTGCGTTGTTTTCATAGCAGCA	CGAGCATCCGTAGTGTGGCCTCTGCGTTGTTTTCATAGCAGCA	NNP
cord-300796-rmjv56ia	109	4	-	-	HYPH
cord-300796-rmjv56ia	109	5	AGGTACACACGGGGGC	AGGTACACACGGGGGC	NNP
cord-300796-rmjv56ia	110	1	TGGAAGCAC	TGGAAGCAC	NNP
cord-300796-rmjv56ia	110	2	GGGAAGGTAGCATTGCJCA	GGGAAGGTAGCATTGCJCA	NNP
cord-300796-rmjv56ia	110	3	-	-	HYPH
cord-300796-rmjv56ia	110	4	AGGAC	AGGAC	NNP
cord-300796-rmjv56ia	110	5	.	.	.
cord-300796-rmjv56ia	111	1	They	-PRON-	PRP
cord-300796-rmjv56ia	111	2	correspond	correspond	VBP
cord-300796-rmjv56ia	111	3	to	to	IN
cord-300796-rmjv56ia	111	4	both	both	DT
cord-300796-rmjv56ia	111	5	strands	strand	NNS
cord-300796-rmjv56ia	111	6	of	of	IN
cord-300796-rmjv56ia	111	7	the	the	DT
cord-300796-rmjv56ia	111	8	p62	p62	NN
cord-300796-rmjv56ia	111	9	signal	signal	NN
cord-300796-rmjv56ia	111	10	sequence	sequence	NN
cord-300796-rmjv56ia	111	11	region	region	NN
cord-300796-rmjv56ia	111	12	of	of	IN
cord-300796-rmjv56ia	111	13	the	the	DT
cord-300796-rmjv56ia	111	14	SFV	sfv	NN
cord-300796-rmjv56ia	112	1	eDNA	eDNA	NNP
cord-300796-rmjv56ia	112	2	.	.	.
cord-300796-rmjv56ia	113	1	Together	together	RB
cord-300796-rmjv56ia	113	2	they	-PRON-	PRP
cord-300796-rmjv56ia	113	3	span	span	VBP
cord-300796-rmjv56ia	113	4	the	the	DT
cord-300796-rmjv56ia	113	5	coding	code	VBG
cord-300796-rmjv56ia	113	6	region	region	NN
cord-300796-rmjv56ia	113	7	of	of	IN
cord-300796-rmjv56ia	113	8	amino	amino	NN
cord-300796-rmjv56ia	113	9	acid	acid	NN
cord-300796-rmjv56ia	113	10	residues	residue	NNS
cord-300796-rmjv56ia	114	1	1	1	LS
cord-300796-rmjv56ia	114	2	-	-	SYM
cord-300796-rmjv56ia	114	3	40	40	CD
cord-300796-rmjv56ia	114	4	of	of	IN
cord-300796-rmjv56ia	114	5	p62	p62	NN
cord-300796-rmjv56ia	114	6	.	.	.
cord-300796-rmjv56ia	115	1	Oligo	Oligo	NNP
cord-300796-rmjv56ia	115	2	1	1	CD
cord-300796-rmjv56ia	115	3	(	(	-LRB-
cord-300796-rmjv56ia	115	4	the	the	DT
cord-300796-rmjv56ia	115	5	coding	code	VBG
cord-300796-rmjv56ia	115	6	strand	strand	NN
cord-300796-rmjv56ia	115	7	)	)	-RRB-
cord-300796-rmjv56ia	115	8	includes	include	VBZ
cord-300796-rmjv56ia	115	9	,	,	,
cord-300796-rmjv56ia	115	10	in	in	IN
cord-300796-rmjv56ia	115	11	addition	addition	NN
cord-300796-rmjv56ia	115	12	,	,	,
cord-300796-rmjv56ia	115	13	the	the	DT
cord-300796-rmjv56ia	115	14	region	region	NN
cord-300796-rmjv56ia	115	15	coding	code	VBG
cord-300796-rmjv56ia	115	16	for	for	IN
cord-300796-rmjv56ia	115	17	initiator	initiator	NN
cord-300796-rmjv56ia	115	18	methionine	methionine	NN
cord-300796-rmjv56ia	115	19	of	of	IN
cord-300796-rmjv56ia	115	20	the	the	DT
cord-300796-rmjv56ia	115	21	C	C	NNP
cord-300796-rmjv56ia	115	22	protein	protein	NN
cord-300796-rmjv56ia	115	23	plus	plus	CC
cord-300796-rmjv56ia	115	24	its	-PRON-	PRP$
cord-300796-rmjv56ia	115	25	5	5	CD
cord-300796-rmjv56ia	115	26	'	'	POS
cord-300796-rmjv56ia	115	27	flanking	flanking	JJ
cord-300796-rmjv56ia	115	28	sequences	sequence	NNS
cord-300796-rmjv56ia	115	29	(	(	-LRB-
cord-300796-rmjv56ia	115	30	5	5	CD
cord-300796-rmjv56ia	115	31	'	'	''
cord-300796-rmjv56ia	115	32	AGCACCATG	AGCACCATG	NNP
cord-300796-rmjv56ia	115	33	)	)	-RRB-
cord-300796-rmjv56ia	115	34	.	.	.
cord-300796-rmjv56ia	116	1	At	at	IN
cord-300796-rmjv56ia	116	2	the	the	DT
cord-300796-rmjv56ia	116	3	extreme	extreme	JJ
cord-300796-rmjv56ia	116	4	5	5	CD
cord-300796-rmjv56ia	116	5	'	'	NN
cord-300796-rmjv56ia	116	6	end	end	NN
cord-300796-rmjv56ia	116	7	of	of	IN
cord-300796-rmjv56ia	116	8	this	this	DT
cord-300796-rmjv56ia	116	9	oligo	oligo	NN
cord-300796-rmjv56ia	116	10	we	-PRON-	PRP
cord-300796-rmjv56ia	116	11	have	have	VBP
cord-300796-rmjv56ia	116	12	added	add	VBN
cord-300796-rmjv56ia	116	13	the	the	DT
cord-300796-rmjv56ia	116	14	recognition	recognition	NN
cord-300796-rmjv56ia	116	15	sequence	sequence	NN
cord-300796-rmjv56ia	116	16	for	for	IN
cord-300796-rmjv56ia	116	17	Eco	Eco	NNP
cord-300796-rmjv56ia	116	18	RI	RI	NNP
cord-300796-rmjv56ia	116	19	and	and	CC
cord-300796-rmjv56ia	116	20	its	-PRON-	PRP$
cord-300796-rmjv56ia	116	21	flanking	flanking	JJ
cord-300796-rmjv56ia	116	22	sequences	sequence	NNS
cord-300796-rmjv56ia	116	23	from	from	IN
cord-300796-rmjv56ia	116	24	the	the	DT
cord-300796-rmjv56ia	116	25	5	5	CD
cord-300796-rmjv56ia	116	26	'	'	NN
cord-300796-rmjv56ia	116	27	end	end	NN
cord-300796-rmjv56ia	116	28	of	of	IN
cord-300796-rmjv56ia	116	29	the	the	DT
cord-300796-rmjv56ia	116	30	structural	structural	JJ
cord-300796-rmjv56ia	116	31	part	part	NN
cord-300796-rmjv56ia	116	32	of	of	IN
cord-300796-rmjv56ia	116	33	the	the	DT
cord-300796-rmjv56ia	116	34	SFV	SFV	NNP
cord-300796-rmjv56ia	116	35	cDNA	cdna	NN
cord-300796-rmjv56ia	116	36	(	(	-LRB-
cord-300796-rmjv56ia	116	37	5	5	CD
cord-300796-rmjv56ia	116	38	'	'	POS
cord-300796-rmjv56ia	116	39	ATACACAGATTC	ATACACAGATTC	NNS
cord-300796-rmjv56ia	116	40	)	)	-RRB-
cord-300796-rmjv56ia	116	41	.	.	.
cord-300796-rmjv56ia	117	1	Oligo	Oligo	NNP
cord-300796-rmjv56ia	117	2	2	2	CD
cord-300796-rmjv56ia	117	3	ends	end	VBZ
cord-300796-rmjv56ia	117	4	at	at	IN
cord-300796-rmjv56ia	117	5	its	-PRON-	PRP$
cord-300796-rmjv56ia	117	6	3	3	CD
cord-300796-rmjv56ia	117	7	'	'	''
cord-300796-rmjv56ia	117	8	end	end	NN
cord-300796-rmjv56ia	117	9	with	with	IN
cord-300796-rmjv56ia	117	10	the	the	DT
cord-300796-rmjv56ia	117	11	Xho	Xho	NNP
cord-300796-rmjv56ia	118	1	I	-PRON-	PRP
cord-300796-rmjv56ia	118	2	site	site	NN
cord-300796-rmjv56ia	118	3	which	which	WDT
cord-300796-rmjv56ia	118	4	follows	follow	VBZ
cord-300796-rmjv56ia	118	5	the	the	DT
cord-300796-rmjv56ia	118	6	signal	signal	NN
cord-300796-rmjv56ia	118	7	sequence	sequence	NN
cord-300796-rmjv56ia	118	8	region	region	NN
cord-300796-rmjv56ia	118	9	on	on	IN
cord-300796-rmjv56ia	118	10	the	the	DT
cord-300796-rmjv56ia	118	11	p62	p62	NN
cord-300796-rmjv56ia	118	12	gene	gene	NN
cord-300796-rmjv56ia	118	13	.	.	.
cord-300796-rmjv56ia	119	1	The	the	DT
cord-300796-rmjv56ia	119	2	two	two	CD
cord-300796-rmjv56ia	119	3	oligonucleotides	oligonucleotide	NNS
cord-300796-rmjv56ia	119	4	were	be	VBD
cord-300796-rmjv56ia	119	5	hybridized	hybridize	VBN
cord-300796-rmjv56ia	119	6	(	(	-LRB-
cord-300796-rmjv56ia	119	7	51	51	CD
cord-300796-rmjv56ia	119	8	complementary	complementary	JJ
cord-300796-rmjv56ia	119	9	bases	basis	NNS
cord-300796-rmjv56ia	119	10	)	)	-RRB-
cord-300796-rmjv56ia	119	11	,	,	,
cord-300796-rmjv56ia	119	12	filled	fill	VBN
cord-300796-rmjv56ia	119	13	in	in	IN
cord-300796-rmjv56ia	119	14	using	use	VBG
cord-300796-rmjv56ia	119	15	Sequenase	Sequenase	NNP
cord-300796-rmjv56ia	119	16	(	(	-LRB-
cord-300796-rmjv56ia	119	17	United	United	NNP
cord-300796-rmjv56ia	119	18	States	States	NNP
cord-300796-rmjv56ia	119	19	Biochemical	Biochemical	NNP
cord-300796-rmjv56ia	119	20	Co.	Co.	NNP
cord-300796-rmjv56ia	119	21	,	,	,
cord-300796-rmjv56ia	119	22	Cleveland	Cleveland	NNP
cord-300796-rmjv56ia	119	23	,	,	,
cord-300796-rmjv56ia	119	24	OH	OH	NNP
cord-300796-rmjv56ia	119	25	)	)	-RRB-
cord-300796-rmjv56ia	119	26	and	and	CC
cord-300796-rmjv56ia	119	27	restricted	restrict	VBN
cord-300796-rmjv56ia	119	28	with	with	IN
cord-300796-rmjv56ia	119	29	Eco	Eco	NNP
cord-300796-rmjv56ia	119	30	RI	RI	NNP
cord-300796-rmjv56ia	119	31	and	and	CC
cord-300796-rmjv56ia	119	32	Xho	Xho	NNP
cord-300796-rmjv56ia	119	33	I.	I.	NNP
cord-300796-rmjv56ia	120	1	The	the	DT
cord-300796-rmjv56ia	120	2	resulting	result	VBG
cord-300796-rmjv56ia	120	3	DNA	dna	NN
cord-300796-rmjv56ia	120	4	fragment	fragment	NN
cord-300796-rmjv56ia	120	5	was	be	VBD
cord-300796-rmjv56ia	120	6	then	then	RB
cord-300796-rmjv56ia	120	7	purified	purify	VBN
cord-300796-rmjv56ia	120	8	and	and	CC
cord-300796-rmjv56ia	120	9	inserted	insert	VBN
cord-300796-rmjv56ia	120	10	into	into	IN
cord-300796-rmjv56ia	120	11	pCp62dhfr	pCp62dhfr	NNP
cord-300796-rmjv56ia	120	12	instead	instead	RB
cord-300796-rmjv56ia	120	13	of	of	IN
cord-300796-rmjv56ia	120	14	the	the	DT
cord-300796-rmjv56ia	120	15	C	C	NNP
cord-300796-rmjv56ia	120	16	and	and	CC
cord-300796-rmjv56ia	120	17	p62	p62	NN
cord-300796-rmjv56ia	120	18	sequences	sequence	NNS
cord-300796-rmjv56ia	120	19	.	.	.
cord-300796-rmjv56ia	121	1	For	for	IN
cord-300796-rmjv56ia	121	2	this	this	DT
cord-300796-rmjv56ia	121	3	purpose	purpose	NN
cord-300796-rmjv56ia	121	4	the	the	DT
cord-300796-rmjv56ia	121	5	pCp62dhfr	pCp62dhfr	NNP
cord-300796-rmjv56ia	121	6	plasmid	plasmid	NN
cord-300796-rmjv56ia	121	7	was	be	VBD
cord-300796-rmjv56ia	121	8	Eco	eco	JJ
cord-300796-rmjv56ia	121	9	RI	RI	NNP
cord-300796-rmjv56ia	121	10	and	and	CC
cord-300796-rmjv56ia	121	11	Xho	Xho	NNP
cord-300796-rmjv56ia	122	1	I	-PRON-	PRP
cord-300796-rmjv56ia	122	2	restricted	restrict	VBD
cord-300796-rmjv56ia	122	3	and	and	CC
cord-300796-rmjv56ia	122	4	the	the	DT
cord-300796-rmjv56ia	122	5	plasmid	plasmid	NN
cord-300796-rmjv56ia	122	6	part	part	NN
cord-300796-rmjv56ia	122	7	with	with	IN
cord-300796-rmjv56ia	122	8	the	the	DT
cord-300796-rmjv56ia	122	9	dhfr	dhfr	NN
cord-300796-rmjv56ia	122	10	sequences	sequence	NNS
cord-300796-rmjv56ia	122	11	isolated	isolate	VBN
cord-300796-rmjv56ia	122	12	.	.	.
cord-300796-rmjv56ia	123	1	The	the	DT
cord-300796-rmjv56ia	123	2	resulting	result	VBG
cord-300796-rmjv56ia	123	3	plasmid	plasmid	NN
cord-300796-rmjv56ia	123	4	p62dhfr	p62dhfr	NN
cord-300796-rmjv56ia	123	5	contains	contain	VBZ
cord-300796-rmjv56ia	123	6	thus	thus	RB
cord-300796-rmjv56ia	123	7	the	the	DT
cord-300796-rmjv56ia	123	8	coding	code	VBG
cord-300796-rmjv56ia	123	9	sequences	sequence	NNS
cord-300796-rmjv56ia	123	10	for	for	IN
cord-300796-rmjv56ia	123	11	the	the	DT
cord-300796-rmjv56ia	123	12	initiator	initiator	NN
cord-300796-rmjv56ia	123	13	methionine	methionine	NN
cord-300796-rmjv56ia	123	14	of	of	IN
cord-300796-rmjv56ia	123	15	C	C	NNP
cord-300796-rmjv56ia	123	16	and	and	CC
cord-300796-rmjv56ia	123	17	the	the	DT
cord-300796-rmjv56ia	123	18	first	first	JJ
cord-300796-rmjv56ia	123	19	40	40	CD
cord-300796-rmjv56ia	123	20	residues	residue	NNS
cord-300796-rmjv56ia	123	21	of	of	IN
cord-300796-rmjv56ia	123	22	the	the	DT
cord-300796-rmjv56ia	123	23	p62	p62	NN
cord-300796-rmjv56ia	123	24	protein	protein	NN
cord-300796-rmjv56ia	123	25	,	,	,
cord-300796-rmjv56ia	123	26	including	include	VBG
cord-300796-rmjv56ia	123	27	the	the	DT
cord-300796-rmjv56ia	123	28	signal	signal	NN
cord-300796-rmjv56ia	123	29	sequence	sequence	NN
cord-300796-rmjv56ia	123	30	,	,	,
cord-300796-rmjv56ia	123	31	in	in	IN
cord-300796-rmjv56ia	123	32	front	front	NN
cord-300796-rmjv56ia	123	33	of	of	IN
cord-300796-rmjv56ia	123	34	the	the	DT
cord-300796-rmjv56ia	123	35	dhfr	dhfr	NN
cord-300796-rmjv56ia	123	36	gene	gene	NN
cord-300796-rmjv56ia	123	37	(	(	-LRB-
cord-300796-rmjv56ia	123	38	see	see	VB
cord-300796-rmjv56ia	123	39	Fig	Fig	NNP
cord-300796-rmjv56ia	123	40	.	.	NNP
cord-300796-rmjv56ia	123	41	1	1	CD
cord-300796-rmjv56ia	123	42	)	)	-RRB-
cord-300796-rmjv56ia	123	43	.	.	.
cord-300796-rmjv56ia	124	1	Construction	construction	NN
cord-300796-rmjv56ia	124	2	of	of	IN
cord-300796-rmjv56ia	124	3	pGEM	pgem	JJ
cord-300796-rmjv56ia	124	4	SFV	sfv	NN
cord-300796-rmjv56ia	124	5	d-4	d-4	NN
cord-300796-rmjv56ia	124	6	.	.	.
cord-300796-rmjv56ia	125	1	This	this	DT
cord-300796-rmjv56ia	125	2	plasmid	plasmid	NN
cord-300796-rmjv56ia	125	3	was	be	VBD
cord-300796-rmjv56ia	125	4	constructed	construct	VBN
cord-300796-rmjv56ia	125	5	by	by	IN
cord-300796-rmjv56ia	125	6	ligatiag	ligatiag	NNP
cord-300796-rmjv56ia	125	7	three	three	CD
cord-300796-rmjv56ia	125	8	fragments	fragment	NNS
cord-300796-rmjv56ia	125	9	together	together	RB
cord-300796-rmjv56ia	125	10	.	.	.
cord-300796-rmjv56ia	126	1	The	the	DT
cord-300796-rmjv56ia	126	2	first	first	JJ
cord-300796-rmjv56ia	126	3	one	one	NN
cord-300796-rmjv56ia	126	4	was	be	VBD
cord-300796-rmjv56ia	126	5	the	the	DT
cord-300796-rmjv56ia	126	6	major	major	JJ
cord-300796-rmjv56ia	126	7	part	part	NN
cord-300796-rmjv56ia	126	8	of	of	IN
cord-300796-rmjv56ia	126	9	pGEM1	pGEM1	NNP
cord-300796-rmjv56ia	126	10	,	,	,
cord-300796-rmjv56ia	126	11	cut	cut	VBN
cord-300796-rmjv56ia	126	12	just	just	RB
cord-300796-rmjv56ia	126	13	after	after	IN
cord-300796-rmjv56ia	126	14	the	the	DT
cord-300796-rmjv56ia	126	15	promoter	promoter	NN
cord-300796-rmjv56ia	126	16	region	region	NN
cord-300796-rmjv56ia	126	17	with	with	IN
cord-300796-rmjv56ia	126	18	Hind	Hind	NNP
cord-300796-rmjv56ia	126	19	III	III	NNP
cord-300796-rmjv56ia	126	20	and	and	CC
cord-300796-rmjv56ia	126	21	Barn	Barn	NNP
cord-300796-rmjv56ia	126	22	HI	HI	NNP
cord-300796-rmjv56ia	126	23	.	.	.
cord-300796-rmjv56ia	127	1	The	the	DT
cord-300796-rmjv56ia	127	2	second	second	JJ
cord-300796-rmjv56ia	127	3	fragment	fragment	NN
cord-300796-rmjv56ia	127	4	(	(	-LRB-
cord-300796-rmjv56ia	127	5	Hind	hind	JJ
cord-300796-rmjv56ia	127	6	I~-Xho	i~-xho	NN
cord-300796-rmjv56ia	127	7	I	i	NN
cord-300796-rmjv56ia	127	8	)	)	-RRB-
cord-300796-rmjv56ia	127	9	was	be	VBD
cord-300796-rmjv56ia	127	10	isolated	isolate	VBN
cord-300796-rmjv56ia	127	11	from	from	IN
cord-300796-rmjv56ia	127	12	the	the	DT
cord-300796-rmjv56ia	127	13	plasmid	plasmid	NN
cord-300796-rmjv56ia	127	14	pSVS	psvs	NN
cord-300796-rmjv56ia	127	15	-	-	HYPH
cord-300796-rmjv56ia	127	16	SFV	sfv	NN
cord-300796-rmjv56ia	127	17	.	.	.
cord-300796-rmjv56ia	128	1	This	this	DT
cord-300796-rmjv56ia	128	2	fragment	fragment	NN
cord-300796-rmjv56ia	128	3	contains	contain	VBZ
cord-300796-rmjv56ia	128	4	the	the	DT
cord-300796-rmjv56ia	128	5	sequences	sequence	NNS
cord-300796-rmjv56ia	128	6	encoding	encode	VBG
cord-300796-rmjv56ia	128	7	the	the	DT
cord-300796-rmjv56ia	128	8	capsid	capsid	NN
cord-300796-rmjv56ia	128	9	and	and	CC
cord-300796-rmjv56ia	128	10	the	the	DT
cord-300796-rmjv56ia	128	11	NH2-terminal	nh2-terminal	JJ
cord-300796-rmjv56ia	128	12	part	part	NN
cord-300796-rmjv56ia	128	13	of	of	IN
cord-300796-rmjv56ia	128	14	the	the	DT
cord-300796-rmjv56ia	128	15	1962	1962	CD
cord-300796-rmjv56ia	128	16	protein	protein	NN
cord-300796-rmjv56ia	128	17	of	of	IN
cord-300796-rmjv56ia	128	18	SFV	SFV	NNP
cord-300796-rmjv56ia	128	19	.	.	.
cord-300796-rmjv56ia	129	1	The	the	DT
cord-300796-rmjv56ia	129	2	third	third	JJ
cord-300796-rmjv56ia	129	3	fragment	fragment	NN
cord-300796-rmjv56ia	129	4	was	be	VBD
cord-300796-rmjv56ia	129	5	obtained	obtain	VBN
cord-300796-rmjv56ia	129	6	by	by	IN
cord-300796-rmjv56ia	129	7	cleaving	cleave	VBG
cord-300796-rmjv56ia	129	8	plasmid	plasmid	NN
cord-300796-rmjv56ia	129	9	pL1	pl1	NN
cord-300796-rmjv56ia	129	10	SFV	sfv	NN
cord-300796-rmjv56ia	129	11	d-4	d-4	NN
cord-300796-rmjv56ia	129	12	(	(	-LRB-
cord-300796-rmjv56ia	129	13	see	see	VB
cord-300796-rmjv56ia	129	14	below	below	RB
cord-300796-rmjv56ia	129	15	)	)	-RRB-
cord-300796-rmjv56ia	129	16	with	with	IN
cord-300796-rmjv56ia	130	1	Xho	Xho	NNP
cord-300796-rmjv56ia	130	2	I	I	NNP
cord-300796-rmjv56ia	130	3	and	and	CC
cord-300796-rmjv56ia	130	4	Barn	Barn	NNP
cord-300796-rmjv56ia	130	5	HI	HI	NNP
cord-300796-rmjv56ia	130	6	and	and	CC
cord-300796-rmjv56ia	130	7	isolating	isolate	VBG
cord-300796-rmjv56ia	130	8	the	the	DT
cord-300796-rmjv56ia	130	9	fragment	fragment	NN
cord-300796-rmjv56ia	130	10	containing	contain	VBG
cord-300796-rmjv56ia	130	11	the	the	DT
cord-300796-rmjv56ia	130	12	3	3	CD
cord-300796-rmjv56ia	130	13	'	'	''
cord-300796-rmjv56ia	130	14	part	part	NN
cord-300796-rmjv56ia	130	15	of	of	IN
cord-300796-rmjv56ia	130	16	the	the	DT
cord-300796-rmjv56ia	130	17	coding	coding	NN
cord-300796-rmjv56ia	130	18	sequence	sequence	NN
cord-300796-rmjv56ia	130	19	for	for	IN
cord-300796-rmjv56ia	130	20	the	the	DT
cord-300796-rmjv56ia	130	21	p62	p62	NN
cord-300796-rmjv56ia	130	22	protein	protein	NN
cord-300796-rmjv56ia	130	23	.	.	.
cord-300796-rmjv56ia	131	1	However	however	RB
cord-300796-rmjv56ia	131	2	,	,	,
cord-300796-rmjv56ia	131	3	it	-PRON-	PRP
cord-300796-rmjv56ia	131	4	should	should	MD
cord-300796-rmjv56ia	131	5	be	be	VB
cord-300796-rmjv56ia	131	6	noted	note	VBN
cord-300796-rmjv56ia	131	7	that	that	IN
cord-300796-rmjv56ia	131	8	in	in	IN
cord-300796-rmjv56ia	131	9	the	the	DT
cord-300796-rmjv56ia	131	10	d-4	d-4	NN
cord-300796-rmjv56ia	131	11	version	version	NN
cord-300796-rmjv56ia	131	12	there	there	EX
cord-300796-rmjv56ia	131	13	is	be	VBZ
cord-300796-rmjv56ia	131	14	an	an	DT
cord-300796-rmjv56ia	131	15	exchange	exchange	NN
cord-300796-rmjv56ia	131	16	of	of	IN
cord-300796-rmjv56ia	131	17	15	15	CD
cord-300796-rmjv56ia	131	18	codons	codon	NNS
cord-300796-rmjv56ia	131	19	at	at	IN
cord-300796-rmjv56ia	131	20	the	the	DT
cord-300796-rmjv56ia	131	21	3	3	CD
cord-300796-rmjv56ia	131	22	'	'	NN
cord-300796-rmjv56ia	131	23	end	end	NN
cord-300796-rmjv56ia	131	24	of	of	IN
cord-300796-rmjv56ia	131	25	the	the	DT
cord-300796-rmjv56ia	131	26	1062	1062	CD
cord-300796-rmjv56ia	131	27	gene	gene	NN
cord-300796-rmjv56ia	131	28	for	for	IN
cord-300796-rmjv56ia	131	29	six	six	CD
cord-300796-rmjv56ia	131	30	aberrant	aberrant	JJ
cord-300796-rmjv56ia	131	31	ones	one	NNS
cord-300796-rmjv56ia	131	32	.	.	.
cord-300796-rmjv56ia	132	1	The	the	DT
cord-300796-rmjv56ia	132	2	corresponding	correspond	VBG
cord-300796-rmjv56ia	132	3	p62	p62	NN
cord-300796-rmjv56ia	132	4	protein	protein	NN
cord-300796-rmjv56ia	132	5	variant	variant	NN
cord-300796-rmjv56ia	132	6	is	be	VBZ
cord-300796-rmjv56ia	132	7	called	call	VBN
cord-300796-rmjv56ia	132	8	1962	1962	CD
cord-300796-rmjv56ia	132	9	d-4	d-4	NN
cord-300796-rmjv56ia	132	10	(	(	-LRB-
cord-300796-rmjv56ia	132	11	see	see	VB
cord-300796-rmjv56ia	132	12	Fig	Fig	NNP
cord-300796-rmjv56ia	132	13	.	.	NNP
cord-300796-rmjv56ia	132	14	1	1	CD
cord-300796-rmjv56ia	132	15	)	)	-RRB-
cord-300796-rmjv56ia	132	16	.	.	.
cord-300796-rmjv56ia	133	1	It	-PRON-	PRP
cord-300796-rmjv56ia	133	2	should	should	MD
cord-300796-rmjv56ia	133	3	also	also	RB
cord-300796-rmjv56ia	133	4	be	be	VB
cord-300796-rmjv56ia	133	5	mentioned	mention	VBN
cord-300796-rmjv56ia	133	6	that	that	IN
cord-300796-rmjv56ia	133	7	pL1	pl1	NN
cord-300796-rmjv56ia	133	8	SFV	SFV	NNP
cord-300796-rmjv56ia	133	9	d-4	d-4	NN
cord-300796-rmjv56ia	133	10	has	have	VBZ
cord-300796-rmjv56ia	133	11	been	be	VBN
cord-300796-rmjv56ia	133	12	derived	derive	VBN
cord-300796-rmjv56ia	133	13	from	from	IN
cord-300796-rmjv56ia	133	14	pL1	pl1	NN
cord-300796-rmjv56ia	133	15	SFV	SFV	NNP
cord-300796-rmjv56ia	133	16	d-9	d-9	NN
cord-300796-rmjv56ia	133	17	,	,	,
cord-300796-rmjv56ia	133	18	(	(	-LRB-
cord-300796-rmjv56ia	133	19	Cutler	Cutler	NNP
cord-300796-rmjv56ia	133	20	and	and	CC
cord-300796-rmjv56ia	133	21	Garoff	Garoff	NNP
cord-300796-rmjv56ia	133	22	,	,	,
cord-300796-rmjv56ia	133	23	1986	1986	CD
cord-300796-rmjv56ia	133	24	)	)	-RRB-
cord-300796-rmjv56ia	133	25	by	by	IN
cord-300796-rmjv56ia	133	26	exchanging	exchange	VBG
cord-300796-rmjv56ia	133	27	the	the	DT
cord-300796-rmjv56ia	133	28	Xho	Xho	NNP
cord-300796-rmjv56ia	133	29	I	I	NNP
cord-300796-rmjv56ia	133	30	-	-	HYPH
cord-300796-rmjv56ia	133	31	Cla	Cla	NNP
cord-300796-rmjv56ia	133	32	I	I	NNP
cord-300796-rmjv56ia	133	33	region	region	NN
cord-300796-rmjv56ia	133	34	containing	contain	VBG
cord-300796-rmjv56ia	133	35	the	the	DT
cord-300796-rmjv56ia	133	36	3	3	CD
cord-300796-rmjv56ia	133	37	'	'	''
cord-300796-rmjv56ia	133	38	part	part	NN
cord-300796-rmjv56ia	133	39	of	of	IN
cord-300796-rmjv56ia	133	40	the	the	DT
cord-300796-rmjv56ia	133	41	p62	p62	NN
cord-300796-rmjv56ia	133	42	coding	code	VBG
cord-300796-rmjv56ia	133	43	region	region	NN
cord-300796-rmjv56ia	133	44	with	with	IN
cord-300796-rmjv56ia	133	45	the	the	DT
cord-300796-rmjv56ia	133	46	similar	similar	JJ
cord-300796-rmjv56ia	133	47	fragment	fragment	NN
cord-300796-rmjv56ia	133	48	from	from	IN
cord-300796-rmjv56ia	133	49	pSV2	pSV2	NNP
cord-300796-rmjv56ia	133	50	SFV	SFV	NNP
cord-300796-rmjv56ia	133	51	d-4	d-4	NN
cord-300796-rmjv56ia	133	52	.	.	.
cord-300796-rmjv56ia	134	1	This	this	DT
cord-300796-rmjv56ia	134	2	latter	latter	JJ
cord-300796-rmjv56ia	134	3	plasmid	plasmid	NN
cord-300796-rmjv56ia	134	4	is	be	VBZ
cord-300796-rmjv56ia	134	5	described	describe	VBN
cord-300796-rmjv56ia	134	6	in	in	IN
cord-300796-rmjv56ia	134	7	Garoff	Garoff	NNP
cord-300796-rmjv56ia	134	8	et	et	NNP
cord-300796-rmjv56ia	134	9	al	al	NNP
cord-300796-rmjv56ia	134	10	.	.	.
cord-300796-rmjv56ia	135	1	(	(	-LRB-
cord-300796-rmjv56ia	135	2	1983	1983	CD
cord-300796-rmjv56ia	135	3	)	)	-RRB-
cord-300796-rmjv56ia	135	4	.	.	.
cord-300796-rmjv56ia	136	1	To	to	TO
cord-300796-rmjv56ia	136	2	define	define	VB
cord-300796-rmjv56ia	136	3	the	the	DT
cord-300796-rmjv56ia	136	4	p62	p62	NN
cord-300796-rmjv56ia	136	5	signal	signal	NN
cord-300796-rmjv56ia	136	6	sequence	sequence	NN
cord-300796-rmjv56ia	136	7	we	-PRON-	PRP
cord-300796-rmjv56ia	136	8	have	have	VBP
cord-300796-rmjv56ia	136	9	studied	study	VBN
cord-300796-rmjv56ia	136	10	the	the	DT
cord-300796-rmjv56ia	136	11	translocation	translocation	NN
cord-300796-rmjv56ia	136	12	phenotype	phenotype	NN
cord-300796-rmjv56ia	136	13	of	of	IN
cord-300796-rmjv56ia	136	14	two	two	CD
cord-300796-rmjv56ia	136	15	reporter	reporter	NN
cord-300796-rmjv56ia	136	16	molecules	molecule	NNS
cord-300796-rmjv56ia	136	17	,	,	,
cord-300796-rmjv56ia	136	18	the	the	DT
cord-300796-rmjv56ia	136	19	rabbit	rabbit	NN
cord-300796-rmjv56ia	136	20	alpha	alpha	NN
cord-300796-rmjv56ia	136	21	-	-	HYPH
cord-300796-rmjv56ia	136	22	globin	globin	NN
cord-300796-rmjv56ia	136	23	and	and	CC
cord-300796-rmjv56ia	136	24	the	the	DT
cord-300796-rmjv56ia	136	25	mouse	mouse	NN
cord-300796-rmjv56ia	136	26	dihydrofolate	dihydrofolate	NNP
cord-300796-rmjv56ia	136	27	reductase	reductase	NNP
cord-300796-rmjv56ia	136	28	(	(	-LRB-
cord-300796-rmjv56ia	136	29	dhfr	dhfr	NNP
cord-300796-rmjv56ia	136	30	)	)	-RRB-
cord-300796-rmjv56ia	136	31	,	,	,
cord-300796-rmjv56ia	136	32	both	both	DT
cord-300796-rmjv56ia	136	33	of	of	IN
cord-300796-rmjv56ia	136	34	which	which	WDT
cord-300796-rmjv56ia	136	35	have	have	VBP
cord-300796-rmjv56ia	136	36	been	be	VBN
cord-300796-rmjv56ia	136	37	extended	extend	VBN
cord-300796-rmjv56ia	136	38	at	at	IN
cord-300796-rmjv56ia	136	39	their	-PRON-	PRP$
cord-300796-rmjv56ia	136	40	NH:-termini	NH:-termini	NNP
cord-300796-rmjv56ia	136	41	with	with	IN
cord-300796-rmjv56ia	136	42	an	an	DT
cord-300796-rmjv56ia	136	43	NH2-terminal	nh2-terminal	NN
cord-300796-rmjv56ia	136	44	40	40	CD
cord-300796-rmjv56ia	136	45	residue	residue	NN
cord-300796-rmjv56ia	136	46	peptide	peptide	NN
cord-300796-rmjv56ia	136	47	from	from	IN
cord-300796-rmjv56ia	136	48	p62	p62	NN
cord-300796-rmjv56ia	136	49	.	.	.
cord-300796-rmjv56ia	137	1	The	the	DT
cord-300796-rmjv56ia	137	2	hybrid	hybrid	JJ
cord-300796-rmjv56ia	137	3	molecules	molecule	NNS
cord-300796-rmjv56ia	137	4	were	be	VBD
cord-300796-rmjv56ia	137	5	tested	test	VBN
cord-300796-rmjv56ia	137	6	in	in	IN
cord-300796-rmjv56ia	137	7	a	a	DT
cord-300796-rmjv56ia	137	8	microsome	microsome	NN
cord-300796-rmjv56ia	137	9	-	-	HYPH
cord-300796-rmjv56ia	137	10	supplemented	supplement	VBN
cord-300796-rmjv56ia	137	11	in	in	IN
cord-300796-rmjv56ia	137	12	vitro	vitro	FW
cord-300796-rmjv56ia	137	13	translation	translation	NN
cord-300796-rmjv56ia	137	14	system	system	NN
cord-300796-rmjv56ia	137	15	.	.	.
cord-300796-rmjv56ia	138	1	The	the	DT
cord-300796-rmjv56ia	138	2	alpha	alpha	NN
cord-300796-rmjv56ia	138	3	-	-	HYPH
cord-300796-rmjv56ia	138	4	globin	globin	NN
cord-300796-rmjv56ia	138	5	and	and	CC
cord-300796-rmjv56ia	138	6	the	the	DT
cord-300796-rmjv56ia	138	7	dhfr	dhfr	NNS
cord-300796-rmjv56ia	138	8	have	have	VBP
cord-300796-rmjv56ia	138	9	earlier	early	RBR
cord-300796-rmjv56ia	138	10	been	be	VBN
cord-300796-rmjv56ia	138	11	shown	show	VBN
cord-300796-rmjv56ia	138	12	to	to	TO
cord-300796-rmjv56ia	138	13	be	be	VB
cord-300796-rmjv56ia	138	14	translocation	translocation	NN
cord-300796-rmjv56ia	138	15	incompetent	incompetent	JJ
cord-300796-rmjv56ia	138	16	if	if	IN
cord-300796-rmjv56ia	138	17	not	not	RB
cord-300796-rmjv56ia	138	18	extended	extend	VBN
cord-300796-rmjv56ia	138	19	with	with	IN
cord-300796-rmjv56ia	138	20	a	a	DT
cord-300796-rmjv56ia	138	21	heterologous	heterologous	JJ
cord-300796-rmjv56ia	138	22	signal	signal	NN
cord-300796-rmjv56ia	138	23	sequence	sequence	NN
cord-300796-rmjv56ia	138	24	at	at	IN
cord-300796-rmjv56ia	138	25	their	-PRON-	PRP$
cord-300796-rmjv56ia	138	26	NH2termini	nh2termini	NN
cord-300796-rmjv56ia	139	1	(	(	-LRB-
cord-300796-rmjv56ia	139	2	Zerial	Zerial	NNP
cord-300796-rmjv56ia	139	3	et	et	FW
cord-300796-rmjv56ia	139	4	al	al	NNP
cord-300796-rmjv56ia	139	5	.	.	NNP
cord-300796-rmjv56ia	139	6	,	,	,
cord-300796-rmjv56ia	139	7	1986	1986	CD
cord-300796-rmjv56ia	139	8	)	)	-RRB-
cord-300796-rmjv56ia	139	9	.	.	.
cord-300796-rmjv56ia	140	1	We	-PRON-	PRP
cord-300796-rmjv56ia	140	2	first	first	RB
cord-300796-rmjv56ia	140	3	tested	test	VBD
cord-300796-rmjv56ia	140	4	the	the	DT
cord-300796-rmjv56ia	140	5	expression	expression	NN
cord-300796-rmjv56ia	140	6	of	of	IN
cord-300796-rmjv56ia	140	7	in	in	FW
cord-300796-rmjv56ia	140	8	vitro	vitro	FW
cord-300796-rmjv56ia	140	9	-	-	HYPH
cord-300796-rmjv56ia	140	10	made	make	VBN
cord-300796-rmjv56ia	140	11	RNA	RNA	NNP
cord-300796-rmjv56ia	140	12	from	from	IN
cord-300796-rmjv56ia	140	13	the	the	DT
cord-300796-rmjv56ia	140	14	construction	construction	NN
cord-300796-rmjv56ia	140	15	pCp62dhfr	pCp62dhfr	NNP
cord-300796-rmjv56ia	140	16	in	in	IN
cord-300796-rmjv56ia	140	17	an	an	DT
cord-300796-rmjv56ia	140	18	in	in	FW
cord-300796-rmjv56ia	140	19	vitro	vitro	FW
cord-300796-rmjv56ia	140	20	translation	translation	NN
cord-300796-rmjv56ia	140	21	system	system	NN
cord-300796-rmjv56ia	140	22	.	.	.
cord-300796-rmjv56ia	141	1	This	this	DT
cord-300796-rmjv56ia	141	2	would	would	MD
cord-300796-rmjv56ia	141	3	be	be	VB
cord-300796-rmjv56ia	141	4	expected	expect	VBN
cord-300796-rmjv56ia	141	5	to	to	TO
cord-300796-rmjv56ia	141	6	yield	yield	VB
cord-300796-rmjv56ia	141	7	free	free	JJ
cord-300796-rmjv56ia	141	8	C	c	NN
cord-300796-rmjv56ia	141	9	protein	protein	NN
cord-300796-rmjv56ia	141	10	and	and	CC
cord-300796-rmjv56ia	141	11	p62reporter	p62report	JJR
cord-300796-rmjv56ia	141	12	hybrid	hybrid	NN
cord-300796-rmjv56ia	141	13	(	(	-LRB-
cord-300796-rmjv56ia	141	14	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	141	15	)	)	-RRB-
cord-300796-rmjv56ia	141	16	through	through	IN
cord-300796-rmjv56ia	141	17	C	c	NN
cord-300796-rmjv56ia	141	18	-	-	HYPH
cord-300796-rmjv56ia	141	19	catalyzed	catalyze	VBN
cord-300796-rmjv56ia	141	20	autoproteolytic	autoproteolytic	JJ
cord-300796-rmjv56ia	141	21	cleavage	cleavage	NN
cord-300796-rmjv56ia	141	22	of	of	IN
cord-300796-rmjv56ia	141	23	the	the	DT
cord-300796-rmjv56ia	141	24	nascent	nascent	JJ
cord-300796-rmjv56ia	141	25	C	C	NNP
cord-300796-rmjv56ia	141	26	-	-	HYPH
cord-300796-rmjv56ia	141	27	p62-reporter	p62-reporter	NN
cord-300796-rmjv56ia	141	28	precursor	precursor	NN
cord-300796-rmjv56ia	141	29	(	(	-LRB-
cord-300796-rmjv56ia	141	30	Fig	Fig	NNP
cord-300796-rmjv56ia	141	31	.	.	.
cord-300796-rmjv56ia	141	32	 	 	_SP
cord-300796-rmjv56ia	142	1	1	1	LS
cord-300796-rmjv56ia	142	2	)	)	-RRB-
cord-300796-rmjv56ia	143	1	(	(	-LRB-
cord-300796-rmjv56ia	143	2	Aliperti	Aliperti	NNP
cord-300796-rmjv56ia	143	3	and	and	CC
cord-300796-rmjv56ia	143	4	Schlesinger	Schlesinger	NNP
cord-300796-rmjv56ia	143	5	,	,	,
cord-300796-rmjv56ia	143	6	1978	1978	CD
cord-300796-rmjv56ia	143	7	;	;	:
cord-300796-rmjv56ia	143	8	Hahn	Hahn	NNP
cord-300796-rmjv56ia	143	9	et	et	NNP
cord-300796-rmjv56ia	143	10	al	al	NNP
cord-300796-rmjv56ia	143	11	.	.	NNP
cord-300796-rmjv56ia	143	12	,	,	,
cord-300796-rmjv56ia	143	13	1985	1985	CD
cord-300796-rmjv56ia	143	14	;	;	:
cord-300796-rmjv56ia	143	15	Melancon	Melancon	NNP
cord-300796-rmjv56ia	143	16	and	and	CC
cord-300796-rmjv56ia	143	17	Garoff	Garoff	NNP
cord-300796-rmjv56ia	143	18	,	,	,
cord-300796-rmjv56ia	143	19	1987	1987	CD
cord-300796-rmjv56ia	143	20	)	)	-RRB-
cord-300796-rmjv56ia	143	21	.	.	.
cord-300796-rmjv56ia	144	1	Furthermore	furthermore	RB
cord-300796-rmjv56ia	144	2	,	,	,
cord-300796-rmjv56ia	144	3	the	the	DT
cord-300796-rmjv56ia	144	4	p62-reporter	p62-reporter	HYPH
cord-300796-rmjv56ia	144	5	hybrid	hybrid	NN
cord-300796-rmjv56ia	144	6	should	should	MD
cord-300796-rmjv56ia	144	7	be	be	VB
cord-300796-rmjv56ia	144	8	translocated	translocate	VBN
cord-300796-rmjv56ia	144	9	across	across	IN
cord-300796-rmjv56ia	144	10	microsomal	microsomal	JJ
cord-300796-rmjv56ia	144	11	membranes	membrane	NNS
cord-300796-rmjv56ia	144	12	and	and	CC
cord-300796-rmjv56ia	144	13	possibly	possibly	RB
cord-300796-rmjv56ia	144	14	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	144	15	at	at	IN
cord-300796-rmjv56ia	144	16	Asn~3	Asn~3	NNP
cord-300796-rmjv56ia	144	17	of	of	IN
cord-300796-rmjv56ia	144	18	the	the	DT
cord-300796-rmjv56ia	144	19	p62	p62	NN
cord-300796-rmjv56ia	144	20	sequence	sequence	NN
cord-300796-rmjv56ia	144	21	if	if	IN
cord-300796-rmjv56ia	144	22	the	the	DT
cord-300796-rmjv56ia	144	23	40	40	CD
cord-300796-rmjv56ia	144	24	residues	residue	NNS
cord-300796-rmjv56ia	144	25	long	long	JJ
cord-300796-rmjv56ia	144	26	NH2-terminal	nh2-terminal	NN
cord-300796-rmjv56ia	144	27	p62	p62	NN
cord-300796-rmjv56ia	144	28	peptide	peptide	NN
cord-300796-rmjv56ia	144	29	carries	carry	VBZ
cord-300796-rmjv56ia	144	30	a	a	DT
cord-300796-rmjv56ia	144	31	signal	signal	JJ
cord-300796-rmjv56ia	144	32	sequence	sequence	NN
cord-300796-rmjv56ia	144	33	.	.	.
cord-300796-rmjv56ia	144	34	 	 	_SP
cord-300796-rmjv56ia	145	1	is	be	VBZ
cord-300796-rmjv56ia	145	2	shown	show	VBN
cord-300796-rmjv56ia	145	3	to	to	TO
cord-300796-rmjv56ia	145	4	be	be	VB
cord-300796-rmjv56ia	145	5	linked	link	VBN
cord-300796-rmjv56ia	145	6	to	to	IN
cord-300796-rmjv56ia	145	7	Asn	Asn	NNP
cord-300796-rmjv56ia	145	8	residue	residue	NN
cord-300796-rmjv56ia	145	9	13	13	CD
cord-300796-rmjv56ia	145	10	in	in	IN
cord-300796-rmjv56ia	145	11	the	the	DT
cord-300796-rmjv56ia	145	12	p62	p62	NN
cord-300796-rmjv56ia	145	13	part	part	NN
cord-300796-rmjv56ia	145	14	(	(	-LRB-
cord-300796-rmjv56ia	145	15	Garoff	Garoff	NNP
cord-300796-rmjv56ia	145	16	et	et	FW
cord-300796-rmjv56ia	145	17	al	al	NNP
cord-300796-rmjv56ia	145	18	.	.	NNP
cord-300796-rmjv56ia	145	19	,	,	,
cord-300796-rmjv56ia	145	20	1982	1982	CD
cord-300796-rmjv56ia	145	21	)	)	-RRB-
cord-300796-rmjv56ia	145	22	.	.	.
cord-300796-rmjv56ia	146	1	Additional	additional	JJ
cord-300796-rmjv56ia	146	2	amino	amino	NN
cord-300796-rmjv56ia	146	3	acids	acid	NNS
cord-300796-rmjv56ia	146	4	resulting	result	VBG
cord-300796-rmjv56ia	146	5	from	from	IN
cord-300796-rmjv56ia	146	6	in	in	IN
cord-300796-rmjv56ia	146	7	frame	frame	NN
cord-300796-rmjv56ia	146	8	translation	translation	NN
cord-300796-rmjv56ia	146	9	of	of	IN
cord-300796-rmjv56ia	146	10	the	the	DT
cord-300796-rmjv56ia	146	11	multicloning	multiclone	VBG
cord-300796-rmjv56ia	146	12	region	region	NN
cord-300796-rmjv56ia	146	13	of	of	IN
cord-300796-rmjv56ia	146	14	pGEM2	pGEM2	NNP
cord-300796-rmjv56ia	146	15	and	and	CC
cord-300796-rmjv56ia	146	16	the	the	DT
cord-300796-rmjv56ia	146	17	added	add	VBN
cord-300796-rmjv56ia	146	18	Xho	Xho	NNP
cord-300796-rmjv56ia	146	19	I	-PRON-	PRP
cord-300796-rmjv56ia	146	20	linker	linker	VBP
cord-300796-rmjv56ia	146	21	as	as	RB
cord-300796-rmjv56ia	146	22	well	well	RB
cord-300796-rmjv56ia	146	23	as	as	IN
cord-300796-rmjv56ia	146	24	the	the	DT
cord-300796-rmjv56ia	146	25	initiator	initiator	NN
cord-300796-rmjv56ia	146	26	Met	Met	NNP
cord-300796-rmjv56ia	146	27	of	of	IN
cord-300796-rmjv56ia	146	28	p62dhfr	p62dhfr	NNP
cord-300796-rmjv56ia	146	29	are	be	VBP
cord-300796-rmjv56ia	146	30	also	also	RB
cord-300796-rmjv56ia	146	31	indicated	indicate	VBN
cord-300796-rmjv56ia	146	32	.	.	.
cord-300796-rmjv56ia	147	1	analysis	analysis	NN
cord-300796-rmjv56ia	147	2	showing	show	VBG
cord-300796-rmjv56ia	147	3	the	the	DT
cord-300796-rmjv56ia	147	4	translocation	translocation	NN
cord-300796-rmjv56ia	147	5	activity	activity	NN
cord-300796-rmjv56ia	147	6	of	of	IN
cord-300796-rmjv56ia	147	7	the	the	DT
cord-300796-rmjv56ia	147	8	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	147	9	protein	protein	NN
cord-300796-rmjv56ia	147	10	.	.	.
cord-300796-rmjv56ia	148	1	In	in	IN
cord-300796-rmjv56ia	148	2	the	the	DT
cord-300796-rmjv56ia	148	3	absence	absence	NN
cord-300796-rmjv56ia	148	4	of	of	IN
cord-300796-rmjv56ia	148	5	membranes	membrane	NNS
cord-300796-rmjv56ia	148	6	(	(	-LRB-
cord-300796-rmjv56ia	148	7	lane/	lane/	CD
cord-300796-rmjv56ia	148	8	)	)	-RRB-
cord-300796-rmjv56ia	149	1	two	two	CD
cord-300796-rmjv56ia	149	2	major	major	JJ
cord-300796-rmjv56ia	149	3	protein	protein	NN
cord-300796-rmjv56ia	149	4	species	specie	NNS
cord-300796-rmjv56ia	149	5	were	be	VBD
cord-300796-rmjv56ia	149	6	translated	translate	VBN
cord-300796-rmjv56ia	149	7	from	from	IN
cord-300796-rmjv56ia	149	8	the	the	DT
cord-300796-rmjv56ia	149	9	SP6-directed	sp6-directe	VBN
cord-300796-rmjv56ia	149	10	transcript	transcript	NN
cord-300796-rmjv56ia	149	11	.	.	.
cord-300796-rmjv56ia	150	1	One	one	CD
cord-300796-rmjv56ia	150	2	of	of	IN
cord-300796-rmjv56ia	150	3	these	these	DT
cord-300796-rmjv56ia	150	4	had	have	VBD
cord-300796-rmjv56ia	150	5	the	the	DT
cord-300796-rmjv56ia	150	6	expected	expect	VBN
cord-300796-rmjv56ia	150	7	size	size	NN
cord-300796-rmjv56ia	150	8	of	of	IN
cord-300796-rmjv56ia	150	9	C	C	NNP
cord-300796-rmjv56ia	150	10	(	(	-LRB-
cord-300796-rmjv56ia	150	11	33	33	CD
cord-300796-rmjv56ia	150	12	kD	kd	JJ
cord-300796-rmjv56ia	150	13	)	)	-RRB-
cord-300796-rmjv56ia	150	14	and	and	CC
cord-300796-rmjv56ia	150	15	the	the	DT
cord-300796-rmjv56ia	150	16	other	other	JJ
cord-300796-rmjv56ia	150	17	one	one	CD
cord-300796-rmjv56ia	150	18	that	that	DT
cord-300796-rmjv56ia	150	19	of	of	IN
cord-300796-rmjv56ia	150	20	the	the	DT
cord-300796-rmjv56ia	150	21	p62-dhfr	p62-dhfr	JJ
cord-300796-rmjv56ia	150	22	hybrid	hybrid	NN
cord-300796-rmjv56ia	150	23	molecule	molecule	NN
cord-300796-rmjv56ia	150	24	(	(	-LRB-
cord-300796-rmjv56ia	150	25	21	21	CD
cord-300796-rmjv56ia	150	26	kD	kd	JJ
cord-300796-rmjv56ia	150	27	)	)	-RRB-
cord-300796-rmjv56ia	150	28	.	.	.
cord-300796-rmjv56ia	151	1	The	the	DT
cord-300796-rmjv56ia	151	2	coding	code	VBG
cord-300796-rmjv56ia	151	3	region	region	NN
cord-300796-rmjv56ia	151	4	has	have	VBZ
cord-300796-rmjv56ia	151	5	apparently	apparently	RB
cord-300796-rmjv56ia	151	6	been	be	VBN
cord-300796-rmjv56ia	151	7	translated	translate	VBN
cord-300796-rmjv56ia	151	8	faithfully	faithfully	RB
cord-300796-rmjv56ia	151	9	and	and	CC
cord-300796-rmjv56ia	151	10	the	the	DT
cord-300796-rmjv56ia	151	11	precursor	precursor	NN
cord-300796-rmjv56ia	151	12	protein	protein	NN
cord-300796-rmjv56ia	151	13	cleaved	cleave	VBD
cord-300796-rmjv56ia	151	14	efficiently	efficiently	RB
cord-300796-rmjv56ia	151	15	.	.	.
cord-300796-rmjv56ia	152	1	The	the	DT
cord-300796-rmjv56ia	152	2	identity	identity	NN
cord-300796-rmjv56ia	152	3	of	of	IN
cord-300796-rmjv56ia	152	4	the	the	DT
cord-300796-rmjv56ia	152	5	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	152	6	was	be	VBD
cord-300796-rmjv56ia	152	7	directly	directly	RB
cord-300796-rmjv56ia	152	8	proven	prove	VBN
cord-300796-rmjv56ia	152	9	by	by	IN
cord-300796-rmjv56ia	152	10	immunoprecipitation	immunoprecipitation	NN
cord-300796-rmjv56ia	152	11	with	with	IN
cord-300796-rmjv56ia	152	12	a	a	DT
cord-300796-rmjv56ia	152	13	dhfr	dhfr	NN
cord-300796-rmjv56ia	152	14	specific	specific	JJ
cord-300796-rmjv56ia	152	15	antiserum	antiserum	NN
cord-300796-rmjv56ia	152	16	(	(	-LRB-
cord-300796-rmjv56ia	152	17	see	see	VB
cord-300796-rmjv56ia	152	18	Fig	Fig	NNP
cord-300796-rmjv56ia	152	19	.	.	.
cord-300796-rmjv56ia	152	20	3	3	CD
cord-300796-rmjv56ia	152	21	)	)	-RRB-
cord-300796-rmjv56ia	152	22	.	.	.
cord-300796-rmjv56ia	153	1	The	the	DT
cord-300796-rmjv56ia	153	2	two	two	CD
cord-300796-rmjv56ia	153	3	weaker	weak	JJR
cord-300796-rmjv56ia	153	4	bands	band	NNS
cord-300796-rmjv56ia	153	5	migrating	migrate	VBG
cord-300796-rmjv56ia	153	6	faster	fast	RBR
cord-300796-rmjv56ia	153	7	than	than	IN
cord-300796-rmjv56ia	153	8	the	the	DT
cord-300796-rmjv56ia	153	9	capsid	capsid	NN
cord-300796-rmjv56ia	153	10	in	in	IN
cord-300796-rmjv56ia	153	11	Fig	Fig	NNP
cord-300796-rmjv56ia	153	12	.	.	.
cord-300796-rmjv56ia	154	1	2	2	LS
cord-300796-rmjv56ia	154	2	,	,	,
cord-300796-rmjv56ia	154	3	lane	lane	NN
cord-300796-rmjv56ia	155	1	I	-PRON-	PRP
cord-300796-rmjv56ia	155	2	were	be	VBD
cord-300796-rmjv56ia	155	3	most	most	RBS
cord-300796-rmjv56ia	155	4	likely	likely	RB
cord-300796-rmjv56ia	155	5	derived	derive	VBN
cord-300796-rmjv56ia	155	6	from	from	IN
cord-300796-rmjv56ia	155	7	C	c	NN
cord-300796-rmjv56ia	155	8	coding	coding	NN
cord-300796-rmjv56ia	155	9	sequences	sequence	NNS
cord-300796-rmjv56ia	155	10	because	because	IN
cord-300796-rmjv56ia	155	11	they	-PRON-	PRP
cord-300796-rmjv56ia	155	12	are	be	VBP
cord-300796-rmjv56ia	155	13	found	find	VBN
cord-300796-rmjv56ia	155	14	in	in	IN
cord-300796-rmjv56ia	155	15	all	all	DT
cord-300796-rmjv56ia	155	16	protein	protein	NN
cord-300796-rmjv56ia	155	17	analyses	analysis	NNS
cord-300796-rmjv56ia	155	18	of	of	IN
cord-300796-rmjv56ia	155	19	in	in	FW
cord-300796-rmjv56ia	155	20	vitro	vitro	FW
cord-300796-rmjv56ia	155	21	transcrip-	transcrip-	XX
cord-300796-rmjv56ia	156	1	Figure	figure	NN
cord-300796-rmjv56ia	156	2	3	3	CD
cord-300796-rmjv56ia	156	3	.	.	.
cord-300796-rmjv56ia	157	1	Immunological	immunological	JJ
cord-300796-rmjv56ia	157	2	identification	identification	NN
cord-300796-rmjv56ia	157	3	of	of	IN
cord-300796-rmjv56ia	157	4	the	the	DT
cord-300796-rmjv56ia	157	5	p62-dhfr	p62-dhfr	JJ
cord-300796-rmjv56ia	157	6	hybrid	hybrid	NN
cord-300796-rmjv56ia	157	7	protein	protein	NN
cord-300796-rmjv56ia	157	8	and	and	CC
cord-300796-rmjv56ia	157	9	analysis	analysis	NN
cord-300796-rmjv56ia	157	10	of	of	IN
cord-300796-rmjv56ia	157	11	its	-PRON-	PRP$
cord-300796-rmjv56ia	157	12	association	association	NN
cord-300796-rmjv56ia	157	13	with	with	IN
cord-300796-rmjv56ia	157	14	membranes	membrane	NNS
cord-300796-rmjv56ia	157	15	.	.	.
cord-300796-rmjv56ia	158	1	RNA	RNA	NNP
cord-300796-rmjv56ia	158	2	transcribed	transcribe	VBD
cord-300796-rmjv56ia	158	3	from	from	IN
cord-300796-rmjv56ia	158	4	pC62dhfr	pC62dhfr	NNP
cord-300796-rmjv56ia	158	5	was	be	VBD
cord-300796-rmjv56ia	158	6	translated	translate	VBN
cord-300796-rmjv56ia	158	7	in	in	IN
cord-300796-rmjv56ia	158	8	vitro	vitro	FW
cord-300796-rmjv56ia	158	9	in	in	IN
cord-300796-rmjv56ia	158	10	the	the	DT
cord-300796-rmjv56ia	158	11	presence	presence	NN
cord-300796-rmjv56ia	158	12	of	of	IN
cord-300796-rmjv56ia	158	13	membranes	membrane	NNS
cord-300796-rmjv56ia	158	14	which	which	WDT
cord-300796-rmjv56ia	158	15	in	in	IN
cord-300796-rmjv56ia	158	16	some	some	DT
cord-300796-rmjv56ia	158	17	cases	case	NNS
cord-300796-rmjv56ia	158	18	had	have	VBD
cord-300796-rmjv56ia	158	19	been	be	VBN
cord-300796-rmjv56ia	158	20	treated	treat	VBN
cord-300796-rmjv56ia	158	21	with	with	IN
cord-300796-rmjv56ia	158	22	an	an	DT
cord-300796-rmjv56ia	158	23	acceptor	acceptor	NN
cord-300796-rmjv56ia	158	24	(	(	-LRB-
cord-300796-rmjv56ia	158	25	Acc	Acc	NNP
cord-300796-rmjv56ia	158	26	)	)	-RRB-
cord-300796-rmjv56ia	158	27	or	or	CC
cord-300796-rmjv56ia	158	28	nonacceptor	nonacceptor	NNP
cord-300796-rmjv56ia	158	29	(	(	-LRB-
cord-300796-rmjv56ia	158	30	Non	Non	NNP
cord-300796-rmjv56ia	158	31	)	)	-RRB-
cord-300796-rmjv56ia	158	32	peptide	peptide	NN
cord-300796-rmjv56ia	158	33	.	.	.
cord-300796-rmjv56ia	159	1	The	the	DT
cord-300796-rmjv56ia	159	2	samples	sample	NNS
cord-300796-rmjv56ia	159	3	were	be	VBD
cord-300796-rmjv56ia	159	4	treated	treat	VBN
cord-300796-rmjv56ia	159	5	,	,	,
cord-300796-rmjv56ia	159	6	after	after	IN
cord-300796-rmjv56ia	159	7	translation	translation	NN
cord-300796-rmjv56ia	159	8	,	,	,
cord-300796-rmjv56ia	159	9	at	at	IN
cord-300796-rmjv56ia	159	10	pH	pH	NNP
cord-300796-rmjv56ia	159	11	11	11	CD
cord-300796-rmjv56ia	159	12	-	-	SYM
cord-300796-rmjv56ia	159	13	11.5	11.5	CD
cord-300796-rmjv56ia	159	14	,	,	,
cord-300796-rmjv56ia	159	15	and	and	CC
cord-300796-rmjv56ia	159	16	the	the	DT
cord-300796-rmjv56ia	159	17	proteins	protein	NNS
cord-300796-rmjv56ia	159	18	then	then	RB
cord-300796-rmjv56ia	159	19	separated	separate	VBD
cord-300796-rmjv56ia	159	20	into	into	IN
cord-300796-rmjv56ia	159	21	a	a	DT
cord-300796-rmjv56ia	159	22	membrane	membrane	NN
cord-300796-rmjv56ia	159	23	-	-	HYPH
cord-300796-rmjv56ia	159	24	bound	bind	VBN
cord-300796-rmjv56ia	159	25	pellet	pellet	NN
cord-300796-rmjv56ia	159	26	fraction	fraction	NN
cord-300796-rmjv56ia	159	27	(	(	-LRB-
cord-300796-rmjv56ia	159	28	P	p	NN
cord-300796-rmjv56ia	159	29	)	)	-RRB-
cord-300796-rmjv56ia	159	30	and	and	CC
cord-300796-rmjv56ia	159	31	a	a	DT
cord-300796-rmjv56ia	159	32	supernatant	supernatant	JJ
cord-300796-rmjv56ia	159	33	fraction	fraction	NN
cord-300796-rmjv56ia	159	34	(	(	-LRB-
cord-300796-rmjv56ia	159	35	S	s	NN
cord-300796-rmjv56ia	159	36	)	)	-RRB-
cord-300796-rmjv56ia	159	37	by	by	IN
cord-300796-rmjv56ia	159	38	centrifugation	centrifugation	NN
cord-300796-rmjv56ia	159	39	.	.	.
cord-300796-rmjv56ia	160	1	In	in	IN
cord-300796-rmjv56ia	160	2	all	all	DT
cord-300796-rmjv56ia	160	3	samples	sample	NNS
cord-300796-rmjv56ia	160	4	the	the	DT
cord-300796-rmjv56ia	160	5	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	160	6	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	160	7	were	be	VBD
cord-300796-rmjv56ia	160	8	isolated	isolate	VBN
cord-300796-rmjv56ia	160	9	using	use	VBG
cord-300796-rmjv56ia	160	10	an	an	DT
cord-300796-rmjv56ia	160	11	anti	anti	JJ
cord-300796-rmjv56ia	160	12	-	-	JJ
cord-300796-rmjv56ia	160	13	dhfr	dhfr	JJ
cord-300796-rmjv56ia	160	14	antibody	antibody	NN
cord-300796-rmjv56ia	160	15	.	.	.
cord-300796-rmjv56ia	161	1	The	the	DT
cord-300796-rmjv56ia	161	2	proteins	protein	NNS
cord-300796-rmjv56ia	161	3	were	be	VBD
cord-300796-rmjv56ia	161	4	then	then	RB
cord-300796-rmjv56ia	161	5	analyzed	analyze	VBN
cord-300796-rmjv56ia	161	6	by	by	IN
cord-300796-rmjv56ia	161	7	SDS	SDS	NNP
cord-300796-rmjv56ia	161	8	-	-	HYPH
cord-300796-rmjv56ia	161	9	PAGE	PAGE	NNP
cord-300796-rmjv56ia	161	10	(	(	-LRB-
cord-300796-rmjv56ia	161	11	10	10	CD
cord-300796-rmjv56ia	161	12	%	%	NN
cord-300796-rmjv56ia	161	13	)	)	-RRB-
cord-300796-rmjv56ia	161	14	and	and	CC
cord-300796-rmjv56ia	161	15	subsequent	subsequent	JJ
cord-300796-rmjv56ia	161	16	autoradiography	autoradiography	NN
cord-300796-rmjv56ia	161	17	.	.	.
cord-300796-rmjv56ia	162	1	The	the	DT
cord-300796-rmjv56ia	162	2	slower	slow	RBR
cord-300796-rmjv56ia	162	3	migrating	migrate	VBG
cord-300796-rmjv56ia	162	4	band	band	NN
cord-300796-rmjv56ia	162	5	corresponds	correspond	VBZ
cord-300796-rmjv56ia	162	6	to	to	IN
cord-300796-rmjv56ia	162	7	glycosylated	glycosylated	NN
cord-300796-rmjv56ia	162	8	and	and	CC
cord-300796-rmjv56ia	162	9	the	the	DT
cord-300796-rmjv56ia	162	10	faster	fast	JJR
cord-300796-rmjv56ia	162	11	one	one	CD
cord-300796-rmjv56ia	162	12	to	to	IN
cord-300796-rmjv56ia	162	13	nonglycosylated	nonglycosylated	JJ
cord-300796-rmjv56ia	162	14	forms	form	NNS
cord-300796-rmjv56ia	162	15	of	of	IN
cord-300796-rmjv56ia	162	16	p62-dhfr	p62-dhfr	NNP
cord-300796-rmjv56ia	162	17	(	(	-LRB-
cord-300796-rmjv56ia	162	18	compare	compare	VB
cord-300796-rmjv56ia	162	19	Fig	Fig	NNP
cord-300796-rmjv56ia	162	20	.	.	.
cord-300796-rmjv56ia	162	21	3	3	CD
cord-300796-rmjv56ia	162	22	)	)	-RRB-
cord-300796-rmjv56ia	162	23	.	.	.
cord-300796-rmjv56ia	163	1	tion	tion	NN
cord-300796-rmjv56ia	163	2	/	/	SYM
cord-300796-rmjv56ia	163	3	translation	translation	NN
cord-300796-rmjv56ia	163	4	mixtures	mixture	NNS
cord-300796-rmjv56ia	163	5	involving	involve	VBG
cord-300796-rmjv56ia	163	6	cDNAs	cDNAs	NNPS
cord-300796-rmjv56ia	163	7	with	with	IN
cord-300796-rmjv56ia	163	8	C	c	NN
cord-300796-rmjv56ia	163	9	regions	region	NNS
cord-300796-rmjv56ia	164	1	(	(	-LRB-
cord-300796-rmjv56ia	164	2	compare	compare	VB
cord-300796-rmjv56ia	164	3	Fig	Fig	NNP
cord-300796-rmjv56ia	164	4	.	.	NNP
cord-300796-rmjv56ia	164	5	6	6	CD
cord-300796-rmjv56ia	164	6	)	)	-RRB-
cord-300796-rmjv56ia	164	7	.	.	.
cord-300796-rmjv56ia	165	1	When	when	WRB
cord-300796-rmjv56ia	165	2	microsomes	microsome	NNS
cord-300796-rmjv56ia	165	3	were	be	VBD
cord-300796-rmjv56ia	165	4	added	add	VBN
cord-300796-rmjv56ia	165	5	to	to	IN
cord-300796-rmjv56ia	165	6	the	the	DT
cord-300796-rmjv56ia	165	7	C	C	NNP
cord-300796-rmjv56ia	165	8	-	-	HYPH
cord-300796-rmjv56ia	165	9	p62-dhfr	p62-dhfr	NNP
cord-300796-rmjv56ia	165	10	in	in	FW
cord-300796-rmjv56ia	165	11	vitro	vitro	FW
cord-300796-rmjv56ia	165	12	translation	translation	NN
cord-300796-rmjv56ia	165	13	system	system	NN
cord-300796-rmjv56ia	165	14	a	a	DT
cord-300796-rmjv56ia	165	15	new	new	JJ
cord-300796-rmjv56ia	165	16	band	band	NN
cord-300796-rmjv56ia	165	17	appeared	appear	VBD
cord-300796-rmjv56ia	165	18	which	which	WDT
cord-300796-rmjv56ia	165	19	migrated	migrate	VBD
cord-300796-rmjv56ia	165	20	somewhat	somewhat	RB
cord-300796-rmjv56ia	165	21	slower	slow	JJR
cord-300796-rmjv56ia	165	22	than	than	IN
cord-300796-rmjv56ia	165	23	the	the	DT
cord-300796-rmjv56ia	165	24	p62-dhfr	p62-dhfr	JJ
cord-300796-rmjv56ia	165	25	band	band	NN
cord-300796-rmjv56ia	165	26	seen	see	VBN
cord-300796-rmjv56ia	165	27	in	in	IN
cord-300796-rmjv56ia	165	28	the	the	DT
cord-300796-rmjv56ia	165	29	analysis	analysis	NN
cord-300796-rmjv56ia	165	30	of	of	IN
cord-300796-rmjv56ia	165	31	the	the	DT
cord-300796-rmjv56ia	165	32	mixture	mixture	NN
cord-300796-rmjv56ia	165	33	lacking	lack	VBG
cord-300796-rmjv56ia	165	34	membranes	membrane	NNS
cord-300796-rmjv56ia	165	35	(	(	-LRB-
cord-300796-rmjv56ia	165	36	Fig	Fig	NNP
cord-300796-rmjv56ia	165	37	.	.	NNP
cord-300796-rmjv56ia	165	38	2	2	CD
cord-300796-rmjv56ia	165	39	,	,	,
cord-300796-rmjv56ia	165	40	lane	lane	NN
cord-300796-rmjv56ia	165	41	2	2	CD
cord-300796-rmjv56ia	165	42	)	)	-RRB-
cord-300796-rmjv56ia	165	43	.	.	.
cord-300796-rmjv56ia	166	1	It	-PRON-	PRP
cord-300796-rmjv56ia	166	2	almost	almost	RB
cord-300796-rmjv56ia	166	3	comigrated	comigrate	VBD
cord-300796-rmjv56ia	166	4	with	with	IN
cord-300796-rmjv56ia	166	5	one	one	CD
cord-300796-rmjv56ia	166	6	of	of	IN
cord-300796-rmjv56ia	166	7	the	the	DT
cord-300796-rmjv56ia	166	8	two	two	CD
cord-300796-rmjv56ia	166	9	weak	weak	JJ
cord-300796-rmjv56ia	166	10	C	c	NN
cord-300796-rmjv56ia	166	11	derived	derive	VBN
cord-300796-rmjv56ia	166	12	bands	band	NNS
cord-300796-rmjv56ia	166	13	.	.	.
cord-300796-rmjv56ia	167	1	The	the	DT
cord-300796-rmjv56ia	167	2	new	new	JJ
cord-300796-rmjv56ia	167	3	band	band	NN
cord-300796-rmjv56ia	167	4	apparently	apparently	RB
cord-300796-rmjv56ia	167	5	corresponds	correspond	VBZ
cord-300796-rmjv56ia	167	6	to	to	IN
cord-300796-rmjv56ia	167	7	IRi2-dhfr	IRi2-dhfr	NNP
cord-300796-rmjv56ia	167	8	hybrids	hybrid	NNS
cord-300796-rmjv56ia	167	9	that	that	WDT
cord-300796-rmjv56ia	167	10	have	have	VBP
cord-300796-rmjv56ia	167	11	been	be	VBN
cord-300796-rmjv56ia	167	12	translocated	translocate	VBN
cord-300796-rmjv56ia	167	13	into	into	IN
cord-300796-rmjv56ia	167	14	the	the	DT
cord-300796-rmjv56ia	167	15	lumen	lumen	NN
cord-300796-rmjv56ia	167	16	of	of	IN
cord-300796-rmjv56ia	167	17	the	the	DT
cord-300796-rmjv56ia	167	18	added	add	VBN
cord-300796-rmjv56ia	167	19	microsomes	microsome	NNS
cord-300796-rmjv56ia	167	20	and	and	CC
cord-300796-rmjv56ia	167	21	have	have	VBP
cord-300796-rmjv56ia	167	22	become	become	VBN
cord-300796-rmjv56ia	167	23	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	167	24	.	.	.
cord-300796-rmjv56ia	168	1	The	the	DT
cord-300796-rmjv56ia	168	2	immunoprecipitation	immunoprecipitation	NN
cord-300796-rmjv56ia	168	3	analysis	analysis	NN
cord-300796-rmjv56ia	168	4	shown	show	VBN
cord-300796-rmjv56ia	168	5	in	in	IN
cord-300796-rmjv56ia	168	6	Fig	Fig	NNP
cord-300796-rmjv56ia	168	7	.	.	.
cord-300796-rmjv56ia	169	1	3	3	CD
cord-300796-rmjv56ia	169	2	confirmed	confirm	VBD
cord-300796-rmjv56ia	169	3	the	the	DT
cord-300796-rmjv56ia	169	4	identity	identity	NN
cord-300796-rmjv56ia	169	5	of	of	IN
cord-300796-rmjv56ia	169	6	this	this	DT
cord-300796-rmjv56ia	169	7	material	material	NN
cord-300796-rmjv56ia	169	8	.	.	.
cord-300796-rmjv56ia	170	1	The	the	DT
cord-300796-rmjv56ia	170	2	protease	protease	NN
cord-300796-rmjv56ia	170	3	digestions	digestion	NNS
cord-300796-rmjv56ia	170	4	in	in	IN
cord-300796-rmjv56ia	170	5	the	the	DT
cord-300796-rmjv56ia	170	6	absence	absence	NN
cord-300796-rmjv56ia	170	7	(	(	-LRB-
cord-300796-rmjv56ia	170	8	Fig	Fig	NNP
cord-300796-rmjv56ia	170	9	.	.	NNP
cord-300796-rmjv56ia	170	10	2	2	CD
cord-300796-rmjv56ia	170	11	,	,	,
cord-300796-rmjv56ia	170	12	lane	lane	NN
cord-300796-rmjv56ia	170	13	 	 	_SP
cord-300796-rmjv56ia	170	14	3	3	CD
cord-300796-rmjv56ia	170	15	)	)	-RRB-
cord-300796-rmjv56ia	170	16	and	and	CC
cord-300796-rmjv56ia	170	17	presence	presence	NN
cord-300796-rmjv56ia	170	18	of	of	IN
cord-300796-rmjv56ia	170	19	Triton	Triton	NNP
cord-300796-rmjv56ia	171	1	X-100	X-100	NNP
cord-300796-rmjv56ia	171	2	(	(	-LRB-
cord-300796-rmjv56ia	171	3	lane	lane	NNP
cord-300796-rmjv56ia	171	4	4	4	CD
cord-300796-rmjv56ia	171	5	)	)	-RRB-
cord-300796-rmjv56ia	171	6	clearly	clearly	RB
cord-300796-rmjv56ia	171	7	demonstrated	demonstrate	VBD
cord-300796-rmjv56ia	171	8	that	that	IN
cord-300796-rmjv56ia	171	9	the	the	DT
cord-300796-rmjv56ia	171	10	slower	slow	RBR
cord-300796-rmjv56ia	171	11	migrating	migrate	VBG
cord-300796-rmjv56ia	171	12	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	171	13	molecules	molecule	NNS
cord-300796-rmjv56ia	171	14	were	be	VBD
cord-300796-rmjv56ia	171	15	indeed	indeed	RB
cord-300796-rmjv56ia	171	16	translocated	translocate	VBN
cord-300796-rmjv56ia	171	17	.	.	.
cord-300796-rmjv56ia	172	1	About	about	RB
cord-300796-rmjv56ia	172	2	half	half	NN
cord-300796-rmjv56ia	172	3	of	of	IN
cord-300796-rmjv56ia	172	4	this	this	DT
cord-300796-rmjv56ia	172	5	material	material	NN
cord-300796-rmjv56ia	172	6	remains	remain	VBZ
cord-300796-rmjv56ia	172	7	protected	protect	VBN
cord-300796-rmjv56ia	172	8	in	in	IN
cord-300796-rmjv56ia	172	9	the	the	DT
cord-300796-rmjv56ia	172	10	presence	presence	NN
cord-300796-rmjv56ia	172	11	of	of	IN
cord-300796-rmjv56ia	172	12	intact	intact	JJ
cord-300796-rmjv56ia	172	13	microsomes	microsome	NNS
cord-300796-rmjv56ia	172	14	whereas	whereas	IN
cord-300796-rmjv56ia	172	15	all	all	DT
cord-300796-rmjv56ia	172	16	is	be	VBZ
cord-300796-rmjv56ia	172	17	digested	digest	VBN
cord-300796-rmjv56ia	172	18	when	when	WRB
cord-300796-rmjv56ia	172	19	the	the	DT
cord-300796-rmjv56ia	172	20	membranes	membrane	NNS
cord-300796-rmjv56ia	172	21	are	be	VBP
cord-300796-rmjv56ia	172	22	solubilized	solubilize	VBN
cord-300796-rmjv56ia	172	23	with	with	IN
cord-300796-rmjv56ia	172	24	detergent	detergent	NN
cord-300796-rmjv56ia	172	25	.	.	.
cord-300796-rmjv56ia	173	1	In	in	IN
cord-300796-rmjv56ia	173	2	contrast	contrast	NN
cord-300796-rmjv56ia	173	3	,	,	,
cord-300796-rmjv56ia	173	4	the	the	DT
cord-300796-rmjv56ia	173	5	other	other	JJ
cord-300796-rmjv56ia	173	6	translated	translate	VBN
cord-300796-rmjv56ia	173	7	material	material	NN
cord-300796-rmjv56ia	173	8	did	do	VBD
cord-300796-rmjv56ia	173	9	not	not	RB
cord-300796-rmjv56ia	173	10	show	show	VB
cord-300796-rmjv56ia	173	11	such	such	PDT
cord-300796-rmjv56ia	173	12	a	a	DT
cord-300796-rmjv56ia	173	13	pronounced	pronounced	JJ
cord-300796-rmjv56ia	173	14	membrane	membrane	NN
cord-300796-rmjv56ia	173	15	-	-	HYPH
cord-300796-rmjv56ia	173	16	dependent	dependent	JJ
cord-300796-rmjv56ia	173	17	protease	protease	NN
cord-300796-rmjv56ia	173	18	resistance	resistance	NN
cord-300796-rmjv56ia	173	19	.	.	.
cord-300796-rmjv56ia	174	1	Note	note	VB
cord-300796-rmjv56ia	174	2	that	that	IN
cord-300796-rmjv56ia	174	3	protease	protease	NN
cord-300796-rmjv56ia	174	4	treatment	treatment	NN
cord-300796-rmjv56ia	174	5	of	of	IN
cord-300796-rmjv56ia	174	6	all	all	DT
cord-300796-rmjv56ia	174	7	samples	sample	NNS
cord-300796-rmjv56ia	174	8	yielded	yield	VBD
cord-300796-rmjv56ia	174	9	a	a	DT
cord-300796-rmjv56ia	174	10	resistant	resistant	JJ
cord-300796-rmjv56ia	174	11	protein	protein	NN
cord-300796-rmjv56ia	174	12	of	of	IN
cord-300796-rmjv56ia	174	13	a	a	DT
cord-300796-rmjv56ia	174	14	small	small	JJ
cord-300796-rmjv56ia	174	15	size	size	NN
cord-300796-rmjv56ia	174	16	.	.	.
cord-300796-rmjv56ia	175	1	This	this	DT
cord-300796-rmjv56ia	175	2	most	most	RBS
cord-300796-rmjv56ia	175	3	likely	likely	RB
cord-300796-rmjv56ia	175	4	represents	represent	VBZ
cord-300796-rmjv56ia	175	5	a	a	DT
cord-300796-rmjv56ia	175	6	protease	protease	NN
cord-300796-rmjv56ia	175	7	-	-	HYPH
cord-300796-rmjv56ia	175	8	resistant	resistant	JJ
cord-300796-rmjv56ia	175	9	C	c	NN
cord-300796-rmjv56ia	175	10	fragment	fragment	NN
cord-300796-rmjv56ia	175	11	.	.	.
cord-300796-rmjv56ia	176	1	The	the	DT
cord-300796-rmjv56ia	176	2	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	176	3	of	of	IN
cord-300796-rmjv56ia	176	4	the	the	DT
cord-300796-rmjv56ia	176	5	translocated	translocate	VBN
cord-300796-rmjv56ia	176	6	p62-hybrid	p62-hybrid	NN
cord-300796-rmjv56ia	176	7	and	and	CC
cord-300796-rmjv56ia	176	8	its	-PRON-	PRP$
cord-300796-rmjv56ia	176	9	effect	effect	NN
cord-300796-rmjv56ia	176	10	on	on	IN
cord-300796-rmjv56ia	176	11	the	the	DT
cord-300796-rmjv56ia	176	12	apparent	apparent	JJ
cord-300796-rmjv56ia	176	13	size	size	NN
cord-300796-rmjv56ia	176	14	of	of	IN
cord-300796-rmjv56ia	176	15	the	the	DT
cord-300796-rmjv56ia	176	16	protein	protein	NN
cord-300796-rmjv56ia	176	17	was	be	VBD
cord-300796-rmjv56ia	176	18	shown	show	VBN
cord-300796-rmjv56ia	176	19	in	in	IN
cord-300796-rmjv56ia	176	20	an	an	DT
cord-300796-rmjv56ia	176	21	experiment	experiment	NN
cord-300796-rmjv56ia	176	22	where	where	WRB
cord-300796-rmjv56ia	176	23	a	a	DT
cord-300796-rmjv56ia	176	24	short	short	JJ
cord-300796-rmjv56ia	176	25	peptide	peptide	NN
cord-300796-rmjv56ia	176	26	(	(	-LRB-
cord-300796-rmjv56ia	176	27	Asn	Asn	NNP
cord-300796-rmjv56ia	176	28	-	-	HYPH
cord-300796-rmjv56ia	176	29	Leu	Leu	NNP
cord-300796-rmjv56ia	176	30	-	-	HYPH
cord-300796-rmjv56ia	176	31	Thr	Thr	NNP
cord-300796-rmjv56ia	176	32	)	)	-RRB-
cord-300796-rmjv56ia	176	33	,	,	,
cord-300796-rmjv56ia	176	34	which	which	WDT
cord-300796-rmjv56ia	176	35	competes	compete	VBZ
cord-300796-rmjv56ia	176	36	for	for	IN
cord-300796-rmjv56ia	176	37	N	n	CD
cord-300796-rmjv56ia	176	38	-	-	HYPH
cord-300796-rmjv56ia	176	39	linked	link	VBN
cord-300796-rmjv56ia	176	40	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	176	41	,	,	,
cord-300796-rmjv56ia	176	42	was	be	VBD
cord-300796-rmjv56ia	176	43	included	include	VBN
cord-300796-rmjv56ia	176	44	during	during	IN
cord-300796-rmjv56ia	176	45	translation	translation	NN
cord-300796-rmjv56ia	176	46	.	.	.
cord-300796-rmjv56ia	177	1	Apparently	apparently	RB
cord-300796-rmjv56ia	177	2	only	only	RB
cord-300796-rmjv56ia	177	3	unglycosylated	unglycosylate	VBN
cord-300796-rmjv56ia	177	4	faster	fast	RBR
cord-300796-rmjv56ia	177	5	migrating	migrate	VBG
cord-300796-rmjv56ia	177	6	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	177	7	hybrids	hybrid	NNS
cord-300796-rmjv56ia	177	8	were	be	VBD
cord-300796-rmjv56ia	177	9	formed	form	VBN
cord-300796-rmjv56ia	177	10	in	in	IN
cord-300796-rmjv56ia	177	11	these	these	DT
cord-300796-rmjv56ia	177	12	conditions	condition	NNS
cord-300796-rmjv56ia	177	13	although	although	IN
cord-300796-rmjv56ia	177	14	chain	chain	NN
cord-300796-rmjv56ia	177	15	translocation	translocation	NN
cord-300796-rmjv56ia	177	16	took	take	VBD
cord-300796-rmjv56ia	177	17	place	place	NN
cord-300796-rmjv56ia	177	18	conferring	confer	VBG
cord-300796-rmjv56ia	177	19	protease	protease	NN
cord-300796-rmjv56ia	177	20	resistance	resistance	NN
cord-300796-rmjv56ia	177	21	(	(	-LRB-
cord-300796-rmjv56ia	177	22	Fig	Fig	NNP
cord-300796-rmjv56ia	177	23	.	.	NNP
cord-300796-rmjv56ia	177	24	2	2	CD
cord-300796-rmjv56ia	177	25	,	,	,
cord-300796-rmjv56ia	177	26	lanes	lane	NNS
cord-300796-rmjv56ia	177	27	5	5	CD
cord-300796-rmjv56ia	177	28	-	-	SYM
cord-300796-rmjv56ia	177	29	7	7	CD
cord-300796-rmjv56ia	177	30	)	)	-RRB-
cord-300796-rmjv56ia	177	31	.	.	.
cord-300796-rmjv56ia	178	1	Additional	additional	JJ
cord-300796-rmjv56ia	178	2	analyses	analysis	NNS
cord-300796-rmjv56ia	178	3	(	(	-LRB-
cord-300796-rmjv56ia	178	4	lanes	lane	NNS
cord-300796-rmjv56ia	178	5	8	8	CD
cord-300796-rmjv56ia	178	6	-	-	SYM
cord-300796-rmjv56ia	178	7	10	10	CD
cord-300796-rmjv56ia	178	8	)	)	-RRB-
cord-300796-rmjv56ia	178	9	illustrate	illustrate	VBP
cord-300796-rmjv56ia	178	10	that	that	IN
cord-300796-rmjv56ia	178	11	a	a	DT
cord-300796-rmjv56ia	178	12	control	control	NN
cord-300796-rmjv56ia	178	13	peptide	peptide	NN
cord-300796-rmjv56ia	178	14	(	(	-LRB-
cord-300796-rmjv56ia	178	15	Asn	Asn	NNP
cord-300796-rmjv56ia	178	16	-	-	HYPH
cord-300796-rmjv56ia	178	17	Leu	Leu	NNP
cord-300796-rmjv56ia	178	18	-	-	HYPH
cord-300796-rmjv56ia	178	19	aThr	aThr	NNP
cord-300796-rmjv56ia	178	20	)	)	-RRB-
cord-300796-rmjv56ia	178	21	which	which	WDT
cord-300796-rmjv56ia	178	22	can	can	MD
cord-300796-rmjv56ia	178	23	not	not	RB
cord-300796-rmjv56ia	178	24	serve	serve	VB
cord-300796-rmjv56ia	178	25	as	as	IN
cord-300796-rmjv56ia	178	26	an	an	DT
cord-300796-rmjv56ia	178	27	acceptor	acceptor	NN
cord-300796-rmjv56ia	178	28	site	site	NN
cord-300796-rmjv56ia	178	29	for	for	IN
cord-300796-rmjv56ia	178	30	N	n	CD
cord-300796-rmjv56ia	178	31	-	-	HYPH
cord-300796-rmjv56ia	178	32	linked	link	VBN
cord-300796-rmjv56ia	178	33	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	178	34	,	,	,
cord-300796-rmjv56ia	178	35	had	have	VBD
cord-300796-rmjv56ia	178	36	no	no	DT
cord-300796-rmjv56ia	178	37	effect	effect	NN
cord-300796-rmjv56ia	178	38	on	on	IN
cord-300796-rmjv56ia	178	39	the	the	DT
cord-300796-rmjv56ia	178	40	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	178	41	of	of	IN
cord-300796-rmjv56ia	178	42	the	the	DT
cord-300796-rmjv56ia	178	43	p62-reporter	p62-reporter	NNS
cord-300796-rmjv56ia	178	44	hybrids	hybrid	NNS
cord-300796-rmjv56ia	178	45	when	when	WRB
cord-300796-rmjv56ia	178	46	tested	test	VBN
cord-300796-rmjv56ia	178	47	in	in	IN
cord-300796-rmjv56ia	178	48	an	an	DT
cord-300796-rmjv56ia	178	49	analogous	analogous	JJ
cord-300796-rmjv56ia	178	50	way	way	NN
cord-300796-rmjv56ia	178	51	.	.	.
cord-300796-rmjv56ia	179	1	Similar	similar	JJ
cord-300796-rmjv56ia	179	2	studies	study	NNS
cord-300796-rmjv56ia	179	3	as	as	IN
cord-300796-rmjv56ia	179	4	with	with	IN
cord-300796-rmjv56ia	179	5	pCp62dhfr	pCp62dhfr	NNP
cord-300796-rmjv56ia	179	6	were	be	VBD
cord-300796-rmjv56ia	179	7	also	also	RB
cord-300796-rmjv56ia	179	8	performed	perform	VBN
cord-300796-rmjv56ia	179	9	with	with	IN
cord-300796-rmjv56ia	179	10	the	the	DT
cord-300796-rmjv56ia	179	11	pCp62globin	pCp62globin	NNP
cord-300796-rmjv56ia	179	12	coded	code	VBN
cord-300796-rmjv56ia	179	13	proteins	protein	NNS
cord-300796-rmjv56ia	179	14	in	in	IN
cord-300796-rmjv56ia	179	15	vitro	vitro	FW
cord-300796-rmjv56ia	179	16	.	.	.
cord-300796-rmjv56ia	180	1	The	the	DT
cord-300796-rmjv56ia	180	2	results	result	NNS
cord-300796-rmjv56ia	180	3	(	(	-LRB-
cord-300796-rmjv56ia	180	4	not	not	RB
cord-300796-rmjv56ia	180	5	shown	show	VBN
cord-300796-rmjv56ia	180	6	)	)	-RRB-
cord-300796-rmjv56ia	180	7	were	be	VBD
cord-300796-rmjv56ia	180	8	analogous	analogous	JJ
cord-300796-rmjv56ia	180	9	to	to	IN
cord-300796-rmjv56ia	180	10	those	those	DT
cord-300796-rmjv56ia	180	11	described	describe	VBN
cord-300796-rmjv56ia	180	12	above	above	RB
cord-300796-rmjv56ia	180	13	for	for	IN
cord-300796-rmjv56ia	180	14	the	the	DT
cord-300796-rmjv56ia	180	15	pCp62dhfr	pCp62dhfr	NNP
cord-300796-rmjv56ia	180	16	construct	construct	VB
cord-300796-rmjv56ia	180	17	.	.	.
cord-300796-rmjv56ia	181	1	C	C	NNP
cord-300796-rmjv56ia	181	2	protein	protein	NN
cord-300796-rmjv56ia	181	3	and	and	CC
cord-300796-rmjv56ia	181	4	p62-globin	p62-globin	CD
cord-300796-rmjv56ia	181	5	hybrid	hybrid	NN
cord-300796-rmjv56ia	181	6	were	be	VBD
cord-300796-rmjv56ia	181	7	synthesized	synthesize	VBN
cord-300796-rmjv56ia	181	8	in	in	IN
cord-300796-rmjv56ia	181	9	the	the	DT
cord-300796-rmjv56ia	181	10	absence	absence	NN
cord-300796-rmjv56ia	181	11	of	of	IN
cord-300796-rmjv56ia	181	12	membranes	membrane	NNS
cord-300796-rmjv56ia	181	13	.	.	.
cord-300796-rmjv56ia	182	1	When	when	WRB
cord-300796-rmjv56ia	182	2	membranes	membrane	NNS
cord-300796-rmjv56ia	182	3	were	be	VBD
cord-300796-rmjv56ia	182	4	added	add	VBN
cord-300796-rmjv56ia	182	5	,	,	,
cord-300796-rmjv56ia	182	6	a	a	DT
cord-300796-rmjv56ia	182	7	protease	protease	NN
cord-300796-rmjv56ia	182	8	-	-	HYPH
cord-300796-rmjv56ia	182	9	protected	protect	VBN
cord-300796-rmjv56ia	182	10	form	form	NN
cord-300796-rmjv56ia	182	11	of	of	IN
cord-300796-rmjv56ia	182	12	the	the	DT
cord-300796-rmjv56ia	182	13	hybrid	hybrid	NN
cord-300796-rmjv56ia	182	14	appeared	appear	VBD
cord-300796-rmjv56ia	182	15	.	.	.
cord-300796-rmjv56ia	183	1	This	this	DT
cord-300796-rmjv56ia	183	2	hybrid	hybrid	NN
cord-300796-rmjv56ia	183	3	was	be	VBD
cord-300796-rmjv56ia	183	4	also	also	RB
cord-300796-rmjv56ia	183	5	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	183	6	as	as	IN
cord-300796-rmjv56ia	183	7	deduced	deduce	VBN
cord-300796-rmjv56ia	183	8	from	from	IN
cord-300796-rmjv56ia	183	9	an	an	DT
cord-300796-rmjv56ia	183	10	experiment	experiment	NN
cord-300796-rmjv56ia	183	11	involving	involve	VBG
cord-300796-rmjv56ia	183	12	the	the	DT
cord-300796-rmjv56ia	183	13	acceptor	acceptor	NN
cord-300796-rmjv56ia	183	14	peptide	peptide	NN
cord-300796-rmjv56ia	183	15	for	for	IN
cord-300796-rmjv56ia	183	16	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	183	17	.	.	.
cord-300796-rmjv56ia	184	1	Fig	fig	NN
cord-300796-rmjv56ia	184	2	.	.	.
cord-300796-rmjv56ia	184	3	3	3	CD
cord-300796-rmjv56ia	185	1	(	(	-LRB-
cord-300796-rmjv56ia	185	2	lanes	lane	NNS
cord-300796-rmjv56ia	185	3	1	1	CD
cord-300796-rmjv56ia	185	4	-	-	SYM
cord-300796-rmjv56ia	185	5	6	6	CD
cord-300796-rmjv56ia	185	6	)	)	-RRB-
cord-300796-rmjv56ia	185	7	shows	show	VBZ
cord-300796-rmjv56ia	185	8	the	the	DT
cord-300796-rmjv56ia	185	9	results	result	NNS
cord-300796-rmjv56ia	185	10	of	of	IN
cord-300796-rmjv56ia	185	11	analyses	analysis	NNS
cord-300796-rmjv56ia	185	12	in	in	IN
cord-300796-rmjv56ia	185	13	which	which	WDT
cord-300796-rmjv56ia	185	14	we	-PRON-	PRP
cord-300796-rmjv56ia	185	15	have	have	VBP
cord-300796-rmjv56ia	185	16	tested	test	VBN
cord-300796-rmjv56ia	185	17	whether	whether	IN
cord-300796-rmjv56ia	185	18	the	the	DT
cord-300796-rmjv56ia	185	19	p62	p62	NN
cord-300796-rmjv56ia	185	20	signal	signal	NN
cord-300796-rmjv56ia	185	21	sequence	sequence	NN
cord-300796-rmjv56ia	185	22	region	region	NN
cord-300796-rmjv56ia	185	23	confers	confer	VBZ
cord-300796-rmjv56ia	185	24	stable	stable	JJ
cord-300796-rmjv56ia	185	25	membrane	membrane	NN
cord-300796-rmjv56ia	185	26	attachment	attachment	NN
cord-300796-rmjv56ia	185	27	to	to	IN
cord-300796-rmjv56ia	185	28	the	the	DT
cord-300796-rmjv56ia	185	29	p62-dhfr	p62-dhfr	JJ
cord-300796-rmjv56ia	185	30	hybrid	hybrid	NN
cord-300796-rmjv56ia	185	31	.	.	.
cord-300796-rmjv56ia	186	1	Microsome	Microsome	NNP
cord-300796-rmjv56ia	186	2	-	-	HYPH
cord-300796-rmjv56ia	186	3	supplemented	supplement	VBN
cord-300796-rmjv56ia	186	4	translations	translation	NNS
cord-300796-rmjv56ia	186	5	were	be	VBD
cord-300796-rmjv56ia	186	6	adjusted	adjust	VBN
cord-300796-rmjv56ia	186	7	to	to	IN
cord-300796-rmjv56ia	186	8	pH	pH	NNP
cord-300796-rmjv56ia	186	9	11	11	CD
cord-300796-rmjv56ia	186	10	-	-	SYM
cord-300796-rmjv56ia	186	11	11.5	11.5	CD
cord-300796-rmjv56ia	186	12	with	with	IN
cord-300796-rmjv56ia	186	13	NaOH	NaOH	NNP
cord-300796-rmjv56ia	186	14	,	,	,
cord-300796-rmjv56ia	186	15	incubated	incubate	VBN
cord-300796-rmjv56ia	186	16	on	on	IN
cord-300796-rmjv56ia	186	17	ice	ice	NN
cord-300796-rmjv56ia	186	18	for	for	IN
cord-300796-rmjv56ia	186	19	10	10	CD
cord-300796-rmjv56ia	186	20	min	min	NN
cord-300796-rmjv56ia	186	21	,	,	,
cord-300796-rmjv56ia	186	22	and	and	CC
cord-300796-rmjv56ia	186	23	then	then	RB
cord-300796-rmjv56ia	186	24	separated	separate	VBD
cord-300796-rmjv56ia	186	25	into	into	IN
cord-300796-rmjv56ia	186	26	a	a	DT
cord-300796-rmjv56ia	186	27	membrane	membrane	NN
cord-300796-rmjv56ia	186	28	pellet	pellet	NN
cord-300796-rmjv56ia	186	29	and	and	CC
cord-300796-rmjv56ia	186	30	supernatant	supernatant	JJ
cord-300796-rmjv56ia	186	31	fraction	fraction	NN
cord-300796-rmjv56ia	186	32	by	by	IN
cord-300796-rmjv56ia	186	33	ultracentrifugation	ultracentrifugation	NN
cord-300796-rmjv56ia	186	34	.	.	.
cord-300796-rmjv56ia	187	1	In	in	IN
cord-300796-rmjv56ia	187	2	all	all	DT
cord-300796-rmjv56ia	187	3	samples	sample	NNS
cord-300796-rmjv56ia	187	4	the	the	DT
cord-300796-rmjv56ia	187	5	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	187	6	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	187	7	were	be	VBD
cord-300796-rmjv56ia	187	8	isolated	isolate	VBN
cord-300796-rmjv56ia	187	9	using	use	VBG
cord-300796-rmjv56ia	187	10	an	an	DT
cord-300796-rmjv56ia	187	11	anti	anti	JJ
cord-300796-rmjv56ia	187	12	-	-	JJ
cord-300796-rmjv56ia	187	13	dhfr	dhfr	JJ
cord-300796-rmjv56ia	187	14	antibody	antibody	NN
cord-300796-rmjv56ia	187	15	,	,	,
cord-300796-rmjv56ia	187	16	SDS	SDS	NNP
cord-300796-rmjv56ia	187	17	-	-	HYPH
cord-300796-rmjv56ia	187	18	PAGE	PAGE	NNP
cord-300796-rmjv56ia	187	19	shows	show	VBZ
cord-300796-rmjv56ia	187	20	that	that	IN
cord-300796-rmjv56ia	187	21	the	the	DT
cord-300796-rmjv56ia	187	22	hybrid	hybrid	JJ
cord-300796-rmjv56ia	187	23	protein	protein	NN
cord-300796-rmjv56ia	187	24	segregates	segregate	VBZ
cord-300796-rmjv56ia	187	25	almost	almost	RB
cord-300796-rmjv56ia	187	26	quantitatively	quantitatively	RB
cord-300796-rmjv56ia	187	27	into	into	IN
cord-300796-rmjv56ia	187	28	the	the	DT
cord-300796-rmjv56ia	187	29	supernatant	supernatant	JJ
cord-300796-rmjv56ia	187	30	fraction	fraction	NN
cord-300796-rmjv56ia	187	31	(	(	-LRB-
cord-300796-rmjv56ia	187	32	compare	compare	VB
cord-300796-rmjv56ia	187	33	lane	lane	NN
cord-300796-rmjv56ia	187	34	I	-PRON-	PRP
cord-300796-rmjv56ia	187	35	with	with	IN
cord-300796-rmjv56ia	187	36	lane	lane	NN
cord-300796-rmjv56ia	187	37	2	2	CD
cord-300796-rmjv56ia	187	38	)	)	-RRB-
cord-300796-rmjv56ia	187	39	.	.	.
cord-300796-rmjv56ia	188	1	In	in	IN
cord-300796-rmjv56ia	188	2	similar	similar	JJ
cord-300796-rmjv56ia	188	3	conditions	condition	NNS
cord-300796-rmjv56ia	188	4	an	an	DT
cord-300796-rmjv56ia	188	5	integral	integral	JJ
cord-300796-rmjv56ia	188	6	membrane	membrane	NN
cord-300796-rmjv56ia	188	7	protein	protein	NN
cord-300796-rmjv56ia	188	8	,	,	,
cord-300796-rmjv56ia	188	9	the	the	DT
cord-300796-rmjv56ia	188	10	human	human	JJ
cord-300796-rmjv56ia	188	11	transferrin	transferrin	NN
cord-300796-rmjv56ia	188	12	receptor	receptor	NN
cord-300796-rmjv56ia	188	13	,	,	,
cord-300796-rmjv56ia	188	14	was	be	VBD
cord-300796-rmjv56ia	188	15	found	find	VBN
cord-300796-rmjv56ia	188	16	to	to	TO
cord-300796-rmjv56ia	188	17	sediment	sediment	VB
cord-300796-rmjv56ia	188	18	with	with	IN
cord-300796-rmjv56ia	188	19	the	the	DT
cord-300796-rmjv56ia	188	20	membranes	membrane	NNS
cord-300796-rmjv56ia	188	21	into	into	IN
cord-300796-rmjv56ia	188	22	the	the	DT
cord-300796-rmjv56ia	188	23	pellet	pellet	NN
cord-300796-rmjv56ia	188	24	fraction	fraction	NN
cord-300796-rmjv56ia	188	25	and	and	CC
cord-300796-rmjv56ia	188	26	a	a	DT
cord-300796-rmjv56ia	188	27	secretory	secretory	JJ
cord-300796-rmjv56ia	188	28	protein	protein	NN
cord-300796-rmjv56ia	188	29	,	,	,
cord-300796-rmjv56ia	188	30	Ig	ig	JJ
cord-300796-rmjv56ia	188	31	light	light	NN
cord-300796-rmjv56ia	188	32	chain	chain	NN
cord-300796-rmjv56ia	188	33	,	,	,
cord-300796-rmjv56ia	188	34	was	be	VBD
cord-300796-rmjv56ia	188	35	only	only	RB
cord-300796-rmjv56ia	188	36	recovered	recover	VBN
cord-300796-rmjv56ia	188	37	in	in	IN
cord-300796-rmjv56ia	188	38	the	the	DT
cord-300796-rmjv56ia	188	39	supernatant	supernatant	NN
cord-300796-rmjv56ia	188	40	(	(	-LRB-
cord-300796-rmjv56ia	188	41	not	not	RB
cord-300796-rmjv56ia	188	42	shown	show	VBN
cord-300796-rmjv56ia	188	43	)	)	-RRB-
cord-300796-rmjv56ia	188	44	.	.	.
cord-300796-rmjv56ia	189	1	If	if	IN
cord-300796-rmjv56ia	189	2	the	the	DT
cord-300796-rmjv56ia	189	3	acceptor	acceptor	NN
cord-300796-rmjv56ia	189	4	peptide	peptide	NN
cord-300796-rmjv56ia	189	5	for	for	IN
cord-300796-rmjv56ia	189	6	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	189	7	was	be	VBD
cord-300796-rmjv56ia	189	8	included	include	VBN
cord-300796-rmjv56ia	189	9	in	in	IN
cord-300796-rmjv56ia	189	10	the	the	DT
cord-300796-rmjv56ia	189	11	in	in	FW
cord-300796-rmjv56ia	189	12	vitro	vitro	FW
cord-300796-rmjv56ia	189	13	translation	translation	NN
cord-300796-rmjv56ia	189	14	and	and	CC
cord-300796-rmjv56ia	189	15	the	the	DT
cord-300796-rmjv56ia	189	16	mixture	mixture	NN
cord-300796-rmjv56ia	189	17	then	then	RB
cord-300796-rmjv56ia	189	18	analyzed	analyze	VBD
cord-300796-rmjv56ia	189	19	we	-PRON-	PRP
cord-300796-rmjv56ia	189	20	found	find	VBD
cord-300796-rmjv56ia	189	21	that	that	IN
cord-300796-rmjv56ia	189	22	the	the	DT
cord-300796-rmjv56ia	189	23	now	now	RB
cord-300796-rmjv56ia	189	24	unglycosylated	unglycosylate	VBN
cord-300796-rmjv56ia	189	25	but	but	CC
cord-300796-rmjv56ia	189	26	still	still	RB
cord-300796-rmjv56ia	189	27	translocated	translocate	VBN
cord-300796-rmjv56ia	189	28	p62-hdfr	p62-hdfr	JJ
cord-300796-rmjv56ia	189	29	hybrids	hybrid	NNS
cord-300796-rmjv56ia	189	30	were	be	VBD
cord-300796-rmjv56ia	189	31	again	again	RB
cord-300796-rmjv56ia	189	32	mostly	mostly	RB
cord-300796-rmjv56ia	189	33	found	find	VBN
cord-300796-rmjv56ia	189	34	in	in	IN
cord-300796-rmjv56ia	189	35	the	the	DT
cord-300796-rmjv56ia	189	36	supernatant	supernatant	JJ
cord-300796-rmjv56ia	189	37	fraction	fraction	NN
cord-300796-rmjv56ia	189	38	(	(	-LRB-
cord-300796-rmjv56ia	189	39	lanes	lane	NNS
cord-300796-rmjv56ia	189	40	3	3	CD
cord-300796-rmjv56ia	189	41	and	and	CC
cord-300796-rmjv56ia	189	42	4	4	CD
cord-300796-rmjv56ia	189	43	)	)	-RRB-
cord-300796-rmjv56ia	189	44	.	.	.
cord-300796-rmjv56ia	190	1	Lanes	lane	NNS
cord-300796-rmjv56ia	190	2	5	5	CD
cord-300796-rmjv56ia	190	3	and	and	CC
cord-300796-rmjv56ia	190	4	6	6	CD
cord-300796-rmjv56ia	190	5	show	show	VBP
cord-300796-rmjv56ia	190	6	the	the	DT
cord-300796-rmjv56ia	190	7	analyses	analysis	NNS
cord-300796-rmjv56ia	190	8	with	with	IN
cord-300796-rmjv56ia	190	9	the	the	DT
cord-300796-rmjv56ia	190	10	control	control	NN
cord-300796-rmjv56ia	190	11	peptide	peptide	NN
cord-300796-rmjv56ia	190	12	.	.	.
cord-300796-rmjv56ia	191	1	To	to	TO
cord-300796-rmjv56ia	191	2	see	see	VB
cord-300796-rmjv56ia	191	3	whether	whether	IN
cord-300796-rmjv56ia	191	4	the	the	DT
cord-300796-rmjv56ia	191	5	C	C	NNP
cord-300796-rmjv56ia	191	6	protein	protein	NN
cord-300796-rmjv56ia	191	7	exerts	exert	VBZ
cord-300796-rmjv56ia	191	8	an	an	DT
cord-300796-rmjv56ia	191	9	influence	influence	NN
cord-300796-rmjv56ia	191	10	on	on	IN
cord-300796-rmjv56ia	191	11	the	the	DT
cord-300796-rmjv56ia	191	12	translation	translation	NN
cord-300796-rmjv56ia	191	13	phenotype	phenotype	NN
cord-300796-rmjv56ia	191	14	of	of	IN
cord-300796-rmjv56ia	191	15	the	the	DT
cord-300796-rmjv56ia	191	16	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	191	17	protein	protein	NN
cord-300796-rmjv56ia	191	18	the	the	DT
cord-300796-rmjv56ia	191	19	p62dhfr	p62dhfr	NN
cord-300796-rmjv56ia	191	20	plasmid	plasmid	NN
cord-300796-rmjv56ia	191	21	(	(	-LRB-
cord-300796-rmjv56ia	191	22	see	see	VB
cord-300796-rmjv56ia	191	23	Fig	Fig	NNP
cord-300796-rmjv56ia	191	24	.	.	NNP
cord-300796-rmjv56ia	191	25	1	1	CD
cord-300796-rmjv56ia	191	26	)	)	-RRB-
cord-300796-rmjv56ia	191	27	,	,	,
cord-300796-rmjv56ia	191	28	lacking	lack	VBG
cord-300796-rmjv56ia	191	29	the	the	DT
cord-300796-rmjv56ia	191	30	C	C	NNP
cord-300796-rmjv56ia	191	31	gene	gene	NN
cord-300796-rmjv56ia	191	32	,	,	,
cord-300796-rmjv56ia	191	33	was	be	VBD
cord-300796-rmjv56ia	191	34	tested	test	VBN
cord-300796-rmjv56ia	191	35	.	.	.
cord-300796-rmjv56ia	192	1	The	the	DT
cord-300796-rmjv56ia	192	2	results	result	NNS
cord-300796-rmjv56ia	192	3	shown	show	VBN
cord-300796-rmjv56ia	192	4	in	in	IN
cord-300796-rmjv56ia	192	5	Fig	Fig	NNP
cord-300796-rmjv56ia	192	6	.	.	.
cord-300796-rmjv56ia	193	1	4	4	LS
cord-300796-rmjv56ia	193	2	show	show	VBP
cord-300796-rmjv56ia	193	3	clearly	clearly	RB
cord-300796-rmjv56ia	193	4	that	that	IN
cord-300796-rmjv56ia	193	5	the	the	DT
cord-300796-rmjv56ia	193	6	p62-dhfr	p62-dhfr	JJ
cord-300796-rmjv56ia	193	7	hybrid	hybrid	NN
cord-300796-rmjv56ia	193	8	is	be	VBZ
cord-300796-rmjv56ia	193	9	translocated	translocate	VBN
cord-300796-rmjv56ia	193	10	and	and	CC
cord-300796-rmjv56ia	193	11	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	193	12	in	in	IN
cord-300796-rmjv56ia	193	13	the	the	DT
cord-300796-rmjv56ia	193	14	same	same	JJ
cord-300796-rmjv56ia	193	15	way	way	NN
cord-300796-rmjv56ia	193	16	as	as	IN
cord-300796-rmjv56ia	193	17	when	when	WRB
cord-300796-rmjv56ia	193	18	expressed	express	VBN
cord-300796-rmjv56ia	193	19	from	from	IN
cord-300796-rmjv56ia	193	20	pCp62dhfr	pCp62dhfr	NNP
cord-300796-rmjv56ia	193	21	.	.	.
cord-300796-rmjv56ia	194	1	Thus	thus	RB
cord-300796-rmjv56ia	194	2	,	,	,
cord-300796-rmjv56ia	194	3	apart	apart	RB
cord-300796-rmjv56ia	194	4	from	from	IN
cord-300796-rmjv56ia	194	5	providing	provide	VBG
cord-300796-rmjv56ia	194	6	a	a	DT
cord-300796-rmjv56ia	194	7	free	free	JJ
cord-300796-rmjv56ia	194	8	NH2-terminal	nh2-terminal	NN
cord-300796-rmjv56ia	194	9	end	end	NN
cord-300796-rmjv56ia	194	10	to	to	IN
cord-300796-rmjv56ia	194	11	the	the	DT
cord-300796-rmjv56ia	194	12	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	194	13	protein	protein	NN
cord-300796-rmjv56ia	194	14	by	by	IN
cord-300796-rmjv56ia	194	15	autoproteolysis	autoproteolysis	NNP
cord-300796-rmjv56ia	194	16	of	of	IN
cord-300796-rmjv56ia	194	17	the	the	DT
cord-300796-rmjv56ia	194	18	C	C	NNP
cord-300796-rmjv56ia	194	19	-	-	HYPH
cord-300796-rmjv56ia	194	20	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	194	21	precursor	precursor	NN
cord-300796-rmjv56ia	194	22	the	the	DT
cord-300796-rmjv56ia	194	23	C	C	NNP
cord-300796-rmjv56ia	194	24	protein	protein	NN
cord-300796-rmjv56ia	194	25	has	have	VBZ
cord-300796-rmjv56ia	194	26	no	no	DT
cord-300796-rmjv56ia	194	27	role	role	NN
cord-300796-rmjv56ia	194	28	in	in	IN
cord-300796-rmjv56ia	194	29	the	the	DT
cord-300796-rmjv56ia	194	30	translocation	translocation	NN
cord-300796-rmjv56ia	194	31	process	process	NN
cord-300796-rmjv56ia	194	32	.	.	.
cord-300796-rmjv56ia	195	1	We	-PRON-	PRP
cord-300796-rmjv56ia	195	2	conclude	conclude	VBP
cord-300796-rmjv56ia	195	3	that	that	IN
cord-300796-rmjv56ia	195	4	the	the	DT
cord-300796-rmjv56ia	195	5	40	40	CD
cord-300796-rmjv56ia	195	6	residue	residue	NN
cord-300796-rmjv56ia	195	7	peptide	peptide	NN
cord-300796-rmjv56ia	195	8	from	from	IN
cord-300796-rmjv56ia	195	9	the	the	DT
cord-300796-rmjv56ia	195	10	p62	p62	NNP
cord-300796-rmjv56ia	195	11	NH2-terminal	NH2-terminal	NNP
cord-300796-rmjv56ia	195	12	region	region	NN
cord-300796-rmjv56ia	195	13	confers	confer	VBZ
cord-300796-rmjv56ia	195	14	a	a	DT
cord-300796-rmjv56ia	195	15	translocation	translocation	NN
cord-300796-rmjv56ia	195	16	positive	positive	JJ
cord-300796-rmjv56ia	195	17	phenotype	phenotype	NN
cord-300796-rmjv56ia	195	18	to	to	IN
cord-300796-rmjv56ia	195	19	the	the	DT
cord-300796-rmjv56ia	195	20	p62-globin	p62-globin	JJ
cord-300796-rmjv56ia	195	21	and	and	CC
cord-300796-rmjv56ia	195	22	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	195	23	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	195	24	and	and	CC
cord-300796-rmjv56ia	195	25	therefore	therefore	RB
cord-300796-rmjv56ia	195	26	must	must	MD
cord-300796-rmjv56ia	195	27	contain	contain	VB
cord-300796-rmjv56ia	195	28	a	a	DT
cord-300796-rmjv56ia	195	29	functional	functional	JJ
cord-300796-rmjv56ia	195	30	signal	signal	NN
cord-300796-rmjv56ia	195	31	sequence	sequence	NN
cord-300796-rmjv56ia	195	32	.	.	.
cord-300796-rmjv56ia	196	1	The	the	DT
cord-300796-rmjv56ia	196	2	translocated	translocate	VBN
cord-300796-rmjv56ia	196	3	fusion	fusion	NN
cord-300796-rmjv56ia	196	4	proteins	protein	NNS
cord-300796-rmjv56ia	196	5	were	be	VBD
cord-300796-rmjv56ia	196	6	also	also	RB
cord-300796-rmjv56ia	196	7	shown	show	VBN
cord-300796-rmjv56ia	196	8	to	to	TO
cord-300796-rmjv56ia	196	9	be	be	VB
cord-300796-rmjv56ia	196	10	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	196	11	.	.	.
cord-300796-rmjv56ia	197	1	This	this	DT
cord-300796-rmjv56ia	197	2	must	must	MD
cord-300796-rmjv56ia	197	3	involve	involve	VB
cord-300796-rmjv56ia	197	4	Asn~3	Asn~3	NNP
cord-300796-rmjv56ia	197	5	of	of	IN
cord-300796-rmjv56ia	197	6	the	the	DT
cord-300796-rmjv56ia	197	7	p62	p62	NN
cord-300796-rmjv56ia	197	8	peptide	peptide	NN
cord-300796-rmjv56ia	197	9	as	as	IN
cord-300796-rmjv56ia	197	10	it	-PRON-	PRP
cord-300796-rmjv56ia	197	11	is	be	VBZ
cord-300796-rmjv56ia	197	12	part	part	NN
cord-300796-rmjv56ia	197	13	of	of	IN
cord-300796-rmjv56ia	197	14	the	the	DT
cord-300796-rmjv56ia	197	15	only	only	JJ
cord-300796-rmjv56ia	197	16	potential	potential	JJ
cord-300796-rmjv56ia	197	17	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	197	18	site	site	NN
cord-300796-rmjv56ia	197	19	on	on	IN
cord-300796-rmjv56ia	197	20	the	the	DT
cord-300796-rmjv56ia	197	21	hybrid	hybrid	NN
cord-300796-rmjv56ia	197	22	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	198	1	(	(	-LRB-
cord-300796-rmjv56ia	198	2	Garoff	Garoff	NNP
cord-300796-rmjv56ia	198	3	et	et	FW
cord-300796-rmjv56ia	198	4	al	al	NNP
cord-300796-rmjv56ia	198	5	.	.	NNP
cord-300796-rmjv56ia	198	6	,	,	,
cord-300796-rmjv56ia	198	7	1980	1980	CD
cord-300796-rmjv56ia	198	8	;	;	:
cord-300796-rmjv56ia	198	9	references	reference	NNS
cord-300796-rmjv56ia	198	10	on	on	IN
cord-300796-rmjv56ia	198	11	dhfr	dhfr	NN
cord-300796-rmjv56ia	198	12	sequence	sequence	NN
cord-300796-rmjv56ia	198	13	in	in	IN
cord-300796-rmjv56ia	198	14	legend	legend	NN
cord-300796-rmjv56ia	198	15	to	to	IN
cord-300796-rmjv56ia	198	16	Fig	Fig	NNP
cord-300796-rmjv56ia	198	17	.	.	.
cord-300796-rmjv56ia	198	18	1	1	CD
cord-300796-rmjv56ia	198	19	)	)	-RRB-
cord-300796-rmjv56ia	198	20	,	,	,
cord-300796-rmjv56ia	198	21	Finally	finally	RB
cord-300796-rmjv56ia	198	22	,	,	,
cord-300796-rmjv56ia	198	23	we	-PRON-	PRP
cord-300796-rmjv56ia	198	24	can	can	MD
cord-300796-rmjv56ia	198	25	also	also	RB
cord-300796-rmjv56ia	198	26	conclude	conclude	VB
cord-300796-rmjv56ia	198	27	that	that	IN
cord-300796-rmjv56ia	198	28	the	the	DT
cord-300796-rmjv56ia	198	29	p62	p62	NN
cord-300796-rmjv56ia	198	30	signal	signal	NN
cord-300796-rmjv56ia	198	31	sequence	sequence	NN
cord-300796-rmjv56ia	198	32	does	do	VBZ
cord-300796-rmjv56ia	198	33	not	not	RB
cord-300796-rmjv56ia	198	34	provide	provide	VB
cord-300796-rmjv56ia	198	35	a	a	DT
cord-300796-rmjv56ia	198	36	stable	stable	JJ
cord-300796-rmjv56ia	198	37	membrane	membrane	NN
cord-300796-rmjv56ia	198	38	anchor	anchor	NN
cord-300796-rmjv56ia	198	39	to	to	IN
cord-300796-rmjv56ia	198	40	the	the	DT
cord-300796-rmjv56ia	198	41	translocated	translocate	VBN
cord-300796-rmjv56ia	198	42	chain	chain	NN
cord-300796-rmjv56ia	198	43	.	.	.
cord-300796-rmjv56ia	199	1	To	to	TO
cord-300796-rmjv56ia	199	2	define	define	VB
cord-300796-rmjv56ia	199	3	at	at	IN
cord-300796-rmjv56ia	199	4	what	what	WDT
cord-300796-rmjv56ia	199	5	time	time	NN
cord-300796-rmjv56ia	199	6	point	point	NN
cord-300796-rmjv56ia	199	7	during	during	IN
cord-300796-rmjv56ia	199	8	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	199	9	chain	chain	NN
cord-300796-rmjv56ia	199	10	synthesis	synthesis	NN
cord-300796-rmjv56ia	199	11	the	the	DT
cord-300796-rmjv56ia	199	12	Asn13	Asn13	NNP
cord-300796-rmjv56ia	199	13	becomes	become	VBZ
cord-300796-rmjv56ia	199	14	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	200	1	we	-PRON-	PRP
cord-300796-rmjv56ia	200	2	performed	perform	VBD
cord-300796-rmjv56ia	200	3	a	a	DT
cord-300796-rmjv56ia	200	4	time	time	NN
cord-300796-rmjv56ia	200	5	-	-	HYPH
cord-300796-rmjv56ia	200	6	course	course	NN
cord-300796-rmjv56ia	200	7	experiment	experiment	NN
cord-300796-rmjv56ia	200	8	essentially	essentially	RB
cord-300796-rmjv56ia	200	9	as	as	IN
cord-300796-rmjv56ia	200	10	described	describe	VBN
cord-300796-rmjv56ia	200	11	by	by	IN
cord-300796-rmjv56ia	200	12	Rothman	Rothman	NNP
cord-300796-rmjv56ia	200	13	and	and	CC
cord-300796-rmjv56ia	200	14	Lodish	Lodish	NNP
cord-300796-rmjv56ia	200	15	(	(	-LRB-
cord-300796-rmjv56ia	200	16	1977	1977	CD
cord-300796-rmjv56ia	200	17	)	)	-RRB-
cord-300796-rmjv56ia	201	1	(	(	-LRB-
cord-300796-rmjv56ia	201	2	Fig	Fig	NNP
cord-300796-rmjv56ia	201	3	.	.	.
cord-300796-rmjv56ia	202	1	5	5	LS
cord-300796-rmjv56ia	202	2	)	)	-RRB-
cord-300796-rmjv56ia	202	3	.	.	.
cord-300796-rmjv56ia	203	1	In	in	IN
cord-300796-rmjv56ia	203	2	this	this	DT
cord-300796-rmjv56ia	203	3	experiment	experiment	NN
cord-300796-rmjv56ia	203	4	a	a	DT
cord-300796-rmjv56ia	203	5	150-#1	150-#1	CD
cord-300796-rmjv56ia	203	6	translation	translation	NN
cord-300796-rmjv56ia	203	7	was	be	VBD
cord-300796-rmjv56ia	203	8	initiated	initiate	VBN
cord-300796-rmjv56ia	203	9	.	.	.
cord-300796-rmjv56ia	204	1	After	after	IN
cord-300796-rmjv56ia	204	2	1.5	1.5	CD
cord-300796-rmjv56ia	204	3	rain	rain	NN
cord-300796-rmjv56ia	204	4	ATA	ATA	NNP
cord-300796-rmjv56ia	204	5	was	be	VBD
cord-300796-rmjv56ia	204	6	added	add	VBN
cord-300796-rmjv56ia	204	7	(	(	-LRB-
cord-300796-rmjv56ia	204	8	0.075	0.075	CD
cord-300796-rmjv56ia	204	9	raM	ram	NN
cord-300796-rmjv56ia	204	10	)	)	-RRB-
cord-300796-rmjv56ia	204	11	to	to	TO
cord-300796-rmjv56ia	204	12	block	block	VB
cord-300796-rmjv56ia	204	13	additional	additional	JJ
cord-300796-rmjv56ia	204	14	starting	starting	NN
cord-300796-rmjv56ia	204	15	of	of	IN
cord-300796-rmjv56ia	204	16	chain	chain	NN
cord-300796-rmjv56ia	204	17	synthesis	synthesis	NN
cord-300796-rmjv56ia	204	18	.	.	.
cord-300796-rmjv56ia	205	1	Then	then	RB
cord-300796-rmjv56ia	205	2	,	,	,
cord-300796-rmjv56ia	205	3	at	at	IN
cord-300796-rmjv56ia	205	4	0.5-rain	0.5-rain	CD
cord-300796-rmjv56ia	205	5	intervals	interval	NNS
cord-300796-rmjv56ia	205	6	,	,	,
cord-300796-rmjv56ia	205	7	two	two	CD
cord-300796-rmjv56ia	205	8	7.5/~1	7.5/~1	CD
cord-300796-rmjv56ia	205	9	aliquots	aliquot	NNS
cord-300796-rmjv56ia	205	10	were	be	VBD
cord-300796-rmjv56ia	205	11	removed	remove	VBN
cord-300796-rmjv56ia	205	12	;	;	:
cord-300796-rmjv56ia	205	13	one	one	CD
cord-300796-rmjv56ia	205	14	for	for	IN
cord-300796-rmjv56ia	205	15	mixing	mix	VBG
cord-300796-rmjv56ia	205	16	with	with	IN
cord-300796-rmjv56ia	205	17	40	40	CD
cord-300796-rmjv56ia	205	18	#	#	$
cord-300796-rmjv56ia	205	19	1	1	CD
cord-300796-rmjv56ia	205	20	of	of	IN
cord-300796-rmjv56ia	205	21	hot	hot	JJ
cord-300796-rmjv56ia	205	22	PAGE	page	NN
cord-300796-rmjv56ia	205	23	sample	sample	NN
cord-300796-rmjv56ia	205	24	buffer	buffer	NN
cord-300796-rmjv56ia	205	25	(	(	-LRB-
cord-300796-rmjv56ia	205	26	2	2	CD
cord-300796-rmjv56ia	205	27	%	%	NN
cord-300796-rmjv56ia	205	28	SDS	SDS	NNP
cord-300796-rmjv56ia	205	29	)	)	-RRB-
cord-300796-rmjv56ia	205	30	and	and	CC
cord-300796-rmjv56ia	205	31	the	the	DT
cord-300796-rmjv56ia	205	32	other	other	JJ
cord-300796-rmjv56ia	205	33	one	one	NN
cord-300796-rmjv56ia	205	34	for	for	IN
cord-300796-rmjv56ia	205	35	further	further	JJ
cord-300796-rmjv56ia	205	36	incubation	incubation	NN
cord-300796-rmjv56ia	205	37	after	after	IN
cord-300796-rmjv56ia	205	38	mixing	mix	VBG
cord-300796-rmjv56ia	205	39	with	with	IN
cord-300796-rmjv56ia	205	40	0.75/~1	0.75/~1	CD
cord-300796-rmjv56ia	205	41	of	of	IN
cord-300796-rmjv56ia	205	42	20	20	CD
cord-300796-rmjv56ia	205	43	%	%	NN
cord-300796-rmjv56ia	205	44	TX-100	TX-100	NNP
cord-300796-rmjv56ia	205	45	.	.	.
cord-300796-rmjv56ia	206	1	The	the	DT
cord-300796-rmjv56ia	206	2	first	first	JJ
cord-300796-rmjv56ia	206	3	sample	sample	NN
cord-300796-rmjv56ia	206	4	from	from	IN
cord-300796-rmjv56ia	206	5	each	each	DT
cord-300796-rmjv56ia	206	6	time	time	NN
cord-300796-rmjv56ia	206	7	point	point	NN
cord-300796-rmjv56ia	206	8	was	be	VBD
cord-300796-rmjv56ia	206	9	used	use	VBN
cord-300796-rmjv56ia	206	10	for	for	IN
cord-300796-rmjv56ia	206	11	the	the	DT
cord-300796-rmjv56ia	206	12	determination	determination	NN
cord-300796-rmjv56ia	206	13	of	of	IN
cord-300796-rmjv56ia	206	14	the	the	DT
cord-300796-rmjv56ia	206	15	time	time	NN
cord-300796-rmjv56ia	206	16	needed	need	VBN
cord-300796-rmjv56ia	206	17	for	for	IN
cord-300796-rmjv56ia	206	18	chain	chain	NN
cord-300796-rmjv56ia	206	19	completion	completion	NN
cord-300796-rmjv56ia	206	20	,	,	,
cord-300796-rmjv56ia	206	21	which	which	WDT
cord-300796-rmjv56ia	206	22	is	be	VBZ
cord-300796-rmjv56ia	206	23	a	a	DT
cord-300796-rmjv56ia	206	24	function	function	NN
cord-300796-rmjv56ia	206	25	of	of	IN
cord-300796-rmjv56ia	206	26	the	the	DT
cord-300796-rmjv56ia	206	27	translation	translation	NN
cord-300796-rmjv56ia	206	28	rate	rate	NN
cord-300796-rmjv56ia	206	29	,	,	,
cord-300796-rmjv56ia	206	30	and	and	CC
cord-300796-rmjv56ia	206	31	the	the	DT
cord-300796-rmjv56ia	206	32	other	other	JJ
cord-300796-rmjv56ia	206	33	one	one	NN
cord-300796-rmjv56ia	206	34	allowed	allow	VBN
cord-300796-rmjv56ia	206	35	determination	determination	NN
cord-300796-rmjv56ia	206	36	of	of	IN
cord-300796-rmjv56ia	206	37	the	the	DT
cord-300796-rmjv56ia	206	38	time	time	NN
cord-300796-rmjv56ia	206	39	course	course	NN
cord-300796-rmjv56ia	206	40	of	of	IN
cord-300796-rmjv56ia	206	41	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	206	42	of	of	IN
cord-300796-rmjv56ia	206	43	the	the	DT
cord-300796-rmjv56ia	206	44	translocated	translocate	VBN
cord-300796-rmjv56ia	206	45	chain	chain	NN
cord-300796-rmjv56ia	206	46	.	.	.
cord-300796-rmjv56ia	207	1	Triton	Triton	NNP
cord-300796-rmjv56ia	207	2	X-100	x-100	IN
cord-300796-rmjv56ia	207	3	solubilizes	solubilizes	NN
cord-300796-rmjv56ia	208	1	the	the	DT
cord-300796-rmjv56ia	208	2	microsomal	microsomal	NNP
cord-300796-rmjv56ia	208	3	membranes	membrane	NNS
cord-300796-rmjv56ia	208	4	and	and	CC
cord-300796-rmjv56ia	208	5	thereby	thereby	RB
cord-300796-rmjv56ia	208	6	inactivates	inactivate	VBZ
cord-300796-rmjv56ia	208	7	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	208	8	(	(	-LRB-
cord-300796-rmjv56ia	208	9	but	but	CC
cord-300796-rmjv56ia	208	10	not	not	RB
cord-300796-rmjv56ia	208	11	chain	chain	NN
cord-300796-rmjv56ia	208	12	elongation	elongation	NN
cord-300796-rmjv56ia	208	13	)	)	-RRB-
cord-300796-rmjv56ia	208	14	.	.	.
cord-300796-rmjv56ia	209	1	Therefore	therefore	RB
cord-300796-rmjv56ia	209	2	,	,	,
cord-300796-rmjv56ia	209	3	only	only	RB
cord-300796-rmjv56ia	209	4	those	those	DT
cord-300796-rmjv56ia	209	5	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	209	6	chains	chain	NNS
cord-300796-rmjv56ia	209	7	that	that	WDT
cord-300796-rmjv56ia	209	8	have	have	VBP
cord-300796-rmjv56ia	209	9	presented	present	VBN
cord-300796-rmjv56ia	209	10	Asnl3	asnl3	JJ
cord-300796-rmjv56ia	209	11	tO	to	IN
cord-300796-rmjv56ia	209	12	the	the	DT
cord-300796-rmjv56ia	209	13	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	209	14	machinery	machinery	NN
cord-300796-rmjv56ia	209	15	before	before	IN
cord-300796-rmjv56ia	209	16	TX-100	TX-100	NNP
cord-300796-rmjv56ia	209	17	addition	addition	NN
cord-300796-rmjv56ia	209	18	have	have	VBP
cord-300796-rmjv56ia	209	19	had	have	VBN
cord-300796-rmjv56ia	209	20	the	the	DT
cord-300796-rmjv56ia	209	21	possibility	possibility	NN
cord-300796-rmjv56ia	209	22	to	to	TO
cord-300796-rmjv56ia	209	23	become	become	VB
cord-300796-rmjv56ia	209	24	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	209	25	.	.	.
cord-300796-rmjv56ia	210	1	In	in	IN
cord-300796-rmjv56ia	210	2	Fig	Fig	NNP
cord-300796-rmjv56ia	210	3	.	.	.
cord-300796-rmjv56ia	211	1	5	5	LS
cord-300796-rmjv56ia	211	2	,	,	,
cord-300796-rmjv56ia	211	3	lanes	lane	NNS
cord-300796-rmjv56ia	211	4	1	1	CD
cord-300796-rmjv56ia	211	5	-	-	SYM
cord-300796-rmjv56ia	211	6	10	10	CD
cord-300796-rmjv56ia	211	7	,	,	,
cord-300796-rmjv56ia	211	8	one	one	PRP
cord-300796-rmjv56ia	211	9	can	can	MD
cord-300796-rmjv56ia	211	10	see	see	VB
cord-300796-rmjv56ia	211	11	that	that	DT
cord-300796-rmjv56ia	211	12	completed	complete	VBN
cord-300796-rmjv56ia	211	13	p62dhfr	p62dhfr	NN
cord-300796-rmjv56ia	211	14	chains	chain	NNS
cord-300796-rmjv56ia	211	15	(	(	-LRB-
cord-300796-rmjv56ia	211	16	197	197	CD
cord-300796-rmjv56ia	211	17	residues	residue	NNS
cord-300796-rmjv56ia	211	18	with	with	IN
cord-300796-rmjv56ia	211	19	initiator	initiator	NN
cord-300796-rmjv56ia	211	20	Met	Met	NNP
cord-300796-rmjv56ia	211	21	)	)	-RRB-
cord-300796-rmjv56ia	211	22	appear	appear	VBP
cord-300796-rmjv56ia	211	23	after	after	IN
cord-300796-rmjv56ia	211	24	a	a	DT
cord-300796-rmjv56ia	211	25	3-min	3-min	JJ
cord-300796-rmjv56ia	211	26	incubation	incubation	NN
cord-300796-rmjv56ia	211	27	from	from	IN
cord-300796-rmjv56ia	211	28	the	the	DT
cord-300796-rmjv56ia	211	29	time	time	NN
cord-300796-rmjv56ia	211	30	point	point	NN
cord-300796-rmjv56ia	211	31	of	of	IN
cord-300796-rmjv56ia	211	32	ATA	ATA	NNP
cord-300796-rmjv56ia	211	33	addition	addition	NN
cord-300796-rmjv56ia	211	34	.	.	.
cord-300796-rmjv56ia	212	1	If	if	IN
cord-300796-rmjv56ia	212	2	one	one	PRP
cord-300796-rmjv56ia	212	3	assumes	assume	VBZ
cord-300796-rmjv56ia	212	4	constant	constant	JJ
cord-300796-rmjv56ia	212	5	chain	chain	NN
cord-300796-rmjv56ia	212	6	initiation	initiation	NN
cord-300796-rmjv56ia	212	7	during	during	IN
cord-300796-rmjv56ia	212	8	the	the	DT
cord-300796-rmjv56ia	212	9	Figure	figure	NN
cord-300796-rmjv56ia	212	10	5	5	CD
cord-300796-rmjv56ia	212	11	.	.	.
cord-300796-rmjv56ia	213	1	Time	time	NN
cord-300796-rmjv56ia	213	2	course	course	NN
cord-300796-rmjv56ia	213	3	of	of	IN
cord-300796-rmjv56ia	213	4	p62	p62	NN
cord-300796-rmjv56ia	213	5	dhfr	dhfr	NN
cord-300796-rmjv56ia	213	6	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	213	7	.	.	.
cord-300796-rmjv56ia	214	1	A	a	DT
cord-300796-rmjv56ia	214	2	150-#1	150-#1	CD
cord-300796-rmjv56ia	214	3	translation	translation	NN
cord-300796-rmjv56ia	214	4	was	be	VBD
cord-300796-rmjv56ia	214	5	initiated	initiate	VBN
cord-300796-rmjv56ia	214	6	.	.	.
cord-300796-rmjv56ia	215	1	After	after	IN
cord-300796-rmjv56ia	215	2	a	a	DT
cord-300796-rmjv56ia	215	3	preincubation	preincubation	NN
cord-300796-rmjv56ia	215	4	time	time	NN
cord-300796-rmjv56ia	215	5	of	of	IN
cord-300796-rmjv56ia	215	6	1.5	1.5	CD
cord-300796-rmjv56ia	215	7	min	min	NN
cord-300796-rmjv56ia	215	8	ATA	ATA	NNP
cord-300796-rmjv56ia	215	9	was	be	VBD
cord-300796-rmjv56ia	215	10	added	add	VBN
cord-300796-rmjv56ia	215	11	to	to	TO
cord-300796-rmjv56ia	215	12	inhibit	inhibit	VB
cord-300796-rmjv56ia	215	13	further	further	JJ
cord-300796-rmjv56ia	215	14	initiation	initiation	NN
cord-300796-rmjv56ia	215	15	of	of	IN
cord-300796-rmjv56ia	215	16	chain	chain	NN
cord-300796-rmjv56ia	215	17	synthesis	synthesis	NN
cord-300796-rmjv56ia	215	18	.	.	.
cord-300796-rmjv56ia	216	1	Then	then	RB
cord-300796-rmjv56ia	216	2	,	,	,
cord-300796-rmjv56ia	216	3	at	at	IN
cord-300796-rmjv56ia	216	4	intervals	interval	NNS
cord-300796-rmjv56ia	216	5	of	of	IN
cord-300796-rmjv56ia	216	6	0.5	0.5	CD
cord-300796-rmjv56ia	216	7	min	min	NN
cord-300796-rmjv56ia	216	8	(	(	-LRB-
cord-300796-rmjv56ia	216	9	indicated	indicate	VBN
cord-300796-rmjv56ia	216	10	by	by	IN
cord-300796-rmjv56ia	216	11	0.5	0.5	NNP
cord-300796-rmjv56ia	216	12	'	'	''
cord-300796-rmjv56ia	216	13	,	,	,
cord-300796-rmjv56ia	216	14	1.0	1.0	CD
cord-300796-rmjv56ia	216	15	'	'	''
cord-300796-rmjv56ia	216	16	,	,	,
cord-300796-rmjv56ia	216	17	1.5	1.5	CD
cord-300796-rmjv56ia	216	18	'	'	POS
cord-300796-rmjv56ia	216	19	,	,	,
cord-300796-rmjv56ia	216	20	2.0	2.0	CD
cord-300796-rmjv56ia	216	21	'	'	POS
cord-300796-rmjv56ia	216	22	,	,	,
cord-300796-rmjv56ia	216	23	2.5	2.5	CD
cord-300796-rmjv56ia	216	24	'	'	''
cord-300796-rmjv56ia	216	25	,	,	,
cord-300796-rmjv56ia	216	26	3.0	3.0	NNP
cord-300796-rmjv56ia	216	27	'	'	POS
cord-300796-rmjv56ia	216	28	,	,	,
cord-300796-rmjv56ia	216	29	3.5	3.5	CD
cord-300796-rmjv56ia	216	30	'	'	POS
cord-300796-rmjv56ia	216	31	,	,	,
cord-300796-rmjv56ia	216	32	4.0	4.0	CD
cord-300796-rmjv56ia	216	33	'	'	POS
cord-300796-rmjv56ia	216	34	,	,	,
cord-300796-rmjv56ia	216	35	4.5	4.5	CD
cord-300796-rmjv56ia	216	36	'	'	''
cord-300796-rmjv56ia	216	37	,	,	,
cord-300796-rmjv56ia	216	38	and	and	CC
cord-300796-rmjv56ia	216	39	5.0	5.0	CD
cord-300796-rmjv56ia	216	40	'	'	''
cord-300796-rmjv56ia	216	41	)	)	-RRB-
cord-300796-rmjv56ia	217	1	two	two	CD
cord-300796-rmjv56ia	217	2	7.5-/zl	7.5-/zl	CD
cord-300796-rmjv56ia	217	3	samples	sample	NNS
cord-300796-rmjv56ia	217	4	were	be	VBD
cord-300796-rmjv56ia	217	5	removed	remove	VBN
cord-300796-rmjv56ia	217	6	,	,	,
cord-300796-rmjv56ia	217	7	one	one	CD
cord-300796-rmjv56ia	217	8	for	for	IN
cord-300796-rmjv56ia	217	9	mixing	mix	VBG
cord-300796-rmjv56ia	217	10	with	with	IN
cord-300796-rmjv56ia	217	11	PAGE	page	NN
cord-300796-rmjv56ia	217	12	sample	sample	NN
cord-300796-rmjv56ia	217	13	buffer	buffer	NN
cord-300796-rmjv56ia	217	14	and	and	CC
cord-300796-rmjv56ia	217	15	another	another	DT
cord-300796-rmjv56ia	217	16	one	one	NN
cord-300796-rmjv56ia	217	17	for	for	IN
cord-300796-rmjv56ia	217	18	mixing	mix	VBG
cord-300796-rmjv56ia	217	19	with	with	IN
cord-300796-rmjv56ia	217	20	TX-100	tx-100	FW
cord-300796-rmjv56ia	217	21	(	(	-LRB-
cord-300796-rmjv56ia	217	22	final	final	JJ
cord-300796-rmjv56ia	217	23	concentration	concentration	NN
cord-300796-rmjv56ia	217	24	1	1	CD
cord-300796-rmjv56ia	217	25	%	%	NN
cord-300796-rmjv56ia	217	26	)	)	-RRB-
cord-300796-rmjv56ia	217	27	and	and	CC
cord-300796-rmjv56ia	217	28	further	further	JJ
cord-300796-rmjv56ia	217	29	incubation	incubation	NN
cord-300796-rmjv56ia	217	30	at	at	IN
cord-300796-rmjv56ia	217	31	30	30	CD
cord-300796-rmjv56ia	217	32	°	°	NN
cord-300796-rmjv56ia	217	33	C	c	NN
cord-300796-rmjv56ia	217	34	(	(	-LRB-
cord-300796-rmjv56ia	217	35	for	for	IN
cord-300796-rmjv56ia	217	36	a	a	DT
cord-300796-rmjv56ia	217	37	total	total	JJ
cord-300796-rmjv56ia	217	38	time	time	NN
cord-300796-rmjv56ia	217	39	of	of	IN
cord-300796-rmjv56ia	217	40	20	20	CD
cord-300796-rmjv56ia	217	41	min	min	NN
cord-300796-rmjv56ia	217	42	after	after	IN
cord-300796-rmjv56ia	217	43	ATA	ATA	NNP
cord-300796-rmjv56ia	217	44	addition	addition	NN
cord-300796-rmjv56ia	217	45	as	as	IN
cord-300796-rmjv56ia	217	46	indicated	indicate	VBN
cord-300796-rmjv56ia	217	47	by	by	IN
cord-300796-rmjv56ia	217	48	the	the	DT
cord-300796-rmjv56ia	217	49	lower	low	JJR
cord-300796-rmjv56ia	217	50	row	row	NN
cord-300796-rmjv56ia	217	51	of	of	IN
cord-300796-rmjv56ia	217	52	time	time	NN
cord-300796-rmjv56ia	217	53	points	point	NNS
cord-300796-rmjv56ia	217	54	in	in	IN
cord-300796-rmjv56ia	217	55	the	the	DT
cord-300796-rmjv56ia	217	56	figure	figure	NN
cord-300796-rmjv56ia	217	57	)	)	-RRB-
cord-300796-rmjv56ia	217	58	.	.	.
cord-300796-rmjv56ia	218	1	Lanes	lane	NNS
cord-300796-rmjv56ia	218	2	1	1	CD
cord-300796-rmjv56ia	218	3	-	-	SYM
cord-300796-rmjv56ia	218	4	10	10	CD
cord-300796-rmjv56ia	218	5	show	show	VBP
cord-300796-rmjv56ia	218	6	the	the	DT
cord-300796-rmjv56ia	218	7	samples	sample	NNS
cord-300796-rmjv56ia	218	8	removed	remove	VBN
cord-300796-rmjv56ia	218	9	for	for	IN
cord-300796-rmjv56ia	218	10	mixing	mix	VBG
cord-300796-rmjv56ia	218	11	with	with	IN
cord-300796-rmjv56ia	218	12	the	the	DT
cord-300796-rmjv56ia	218	13	PAGE	page	NN
cord-300796-rmjv56ia	218	14	sample	sample	NN
cord-300796-rmjv56ia	218	15	buffer	buffer	NN
cord-300796-rmjv56ia	218	16	.	.	.
cord-300796-rmjv56ia	219	1	From	from	IN
cord-300796-rmjv56ia	219	2	these	these	DT
cord-300796-rmjv56ia	219	3	results	result	NNS
cord-300796-rmjv56ia	219	4	the	the	DT
cord-300796-rmjv56ia	219	5	approximate	approximate	JJ
cord-300796-rmjv56ia	219	6	rate	rate	NN
cord-300796-rmjv56ia	219	7	of	of	IN
cord-300796-rmjv56ia	219	8	translation	translation	NN
cord-300796-rmjv56ia	219	9	can	can	MD
cord-300796-rmjv56ia	219	10	be	be	VB
cord-300796-rmjv56ia	219	11	derived	derive	VBN
cord-300796-rmjv56ia	219	12	.	.	.
cord-300796-rmjv56ia	220	1	Completed	complete	VBN
cord-300796-rmjv56ia	220	2	chains	chain	NNS
cord-300796-rmjv56ia	220	3	appear	appear	VBP
cord-300796-rmjv56ia	220	4	in	in	IN
cord-300796-rmjv56ia	220	5	the	the	DT
cord-300796-rmjv56ia	220	6	3-min	3-min	JJ
cord-300796-rmjv56ia	220	7	sample	sample	NN
cord-300796-rmjv56ia	220	8	.	.	.
cord-300796-rmjv56ia	221	1	Lanes	lane	NNS
cord-300796-rmjv56ia	221	2	11	11	CD
cord-300796-rmjv56ia	221	3	-	-	SYM
cord-300796-rmjv56ia	221	4	20	20	CD
cord-300796-rmjv56ia	221	5	show	show	VBP
cord-300796-rmjv56ia	221	6	the	the	DT
cord-300796-rmjv56ia	221	7	samples	sample	NNS
cord-300796-rmjv56ia	221	8	in	in	IN
cord-300796-rmjv56ia	221	9	which	which	WDT
cord-300796-rmjv56ia	221	10	the	the	DT
cord-300796-rmjv56ia	221	11	membranes	membrane	NNS
cord-300796-rmjv56ia	221	12	have	have	VBP
cord-300796-rmjv56ia	221	13	been	be	VBN
cord-300796-rmjv56ia	221	14	solubilized	solubilize	VBN
cord-300796-rmjv56ia	221	15	with	with	IN
cord-300796-rmjv56ia	221	16	Triton	Triton	NNP
cord-300796-rmjv56ia	221	17	X-100	x-100	NN
cord-300796-rmjv56ia	221	18	for	for	IN
cord-300796-rmjv56ia	221	19	inactivation	inactivation	NN
cord-300796-rmjv56ia	221	20	of	of	IN
cord-300796-rmjv56ia	221	21	the	the	DT
cord-300796-rmjv56ia	221	22	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	221	23	machinery	machinery	NN
cord-300796-rmjv56ia	221	24	.	.	.
cord-300796-rmjv56ia	222	1	From	from	IN
cord-300796-rmjv56ia	222	2	these	these	DT
cord-300796-rmjv56ia	222	3	analyses	analysis	NNS
cord-300796-rmjv56ia	222	4	it	-PRON-	PRP
cord-300796-rmjv56ia	222	5	is	be	VBZ
cord-300796-rmjv56ia	222	6	possible	possible	JJ
cord-300796-rmjv56ia	222	7	to	to	TO
cord-300796-rmjv56ia	222	8	estimate	estimate	VB
cord-300796-rmjv56ia	222	9	when	when	WRB
cord-300796-rmjv56ia	222	10	Asn13	Asn13	NNP
cord-300796-rmjv56ia	222	11	is	be	VBZ
cord-300796-rmjv56ia	222	12	modified	modify	VBN
cord-300796-rmjv56ia	222	13	during	during	IN
cord-300796-rmjv56ia	222	14	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	222	15	synthesis	synthesis	NN
cord-300796-rmjv56ia	222	16	.	.	.
cord-300796-rmjv56ia	223	1	The	the	DT
cord-300796-rmjv56ia	223	2	first	first	JJ
cord-300796-rmjv56ia	223	3	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	223	4	forms	form	NNS
cord-300796-rmjv56ia	223	5	are	be	VBP
cord-300796-rmjv56ia	223	6	clearly	clearly	RB
cord-300796-rmjv56ia	223	7	visible	visible	JJ
cord-300796-rmjv56ia	223	8	in	in	IN
cord-300796-rmjv56ia	223	9	the	the	DT
cord-300796-rmjv56ia	223	10	1.5-min	1.5-min	CD
cord-300796-rmjv56ia	223	11	sample	sample	NN
cord-300796-rmjv56ia	223	12	,	,	,
cord-300796-rmjv56ia	223	13	a	a	DT
cord-300796-rmjv56ia	223	14	time	time	NN
cord-300796-rmjv56ia	223	15	point	point	NN
cord-300796-rmjv56ia	223	16	where	where	WRB
cord-300796-rmjv56ia	223	17	only	only	RB
cord-300796-rmjv56ia	223	18	about	about	IN
cord-300796-rmjv56ia	223	19	half	half	NN
cord-300796-rmjv56ia	223	20	of	of	IN
cord-300796-rmjv56ia	223	21	the	the	DT
cord-300796-rmjv56ia	223	22	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	223	23	chain	chain	NN
cord-300796-rmjv56ia	223	24	has	have	VBZ
cord-300796-rmjv56ia	223	25	been	be	VBN
cord-300796-rmjv56ia	223	26	synthesized	synthesize	VBN
cord-300796-rmjv56ia	223	27	.	.	.
cord-300796-rmjv56ia	224	1	The	the	DT
cord-300796-rmjv56ia	224	2	nature	nature	NN
cord-300796-rmjv56ia	224	3	of	of	IN
cord-300796-rmjv56ia	224	4	the	the	DT
cord-300796-rmjv56ia	224	5	material	material	NN
cord-300796-rmjv56ia	224	6	in	in	IN
cord-300796-rmjv56ia	224	7	the	the	DT
cord-300796-rmjv56ia	224	8	two	two	CD
cord-300796-rmjv56ia	224	9	weak	weak	JJ
cord-300796-rmjv56ia	224	10	bands	band	NNS
cord-300796-rmjv56ia	224	11	seen	see	VBN
cord-300796-rmjv56ia	224	12	in	in	IN
cord-300796-rmjv56ia	224	13	lanes	lane	NNS
cord-300796-rmjv56ia	224	14	1	1	CD
cord-300796-rmjv56ia	224	15	-	-	SYM
cord-300796-rmjv56ia	224	16	5	5	CD
cord-300796-rmjv56ia	224	17	is	be	VBZ
cord-300796-rmjv56ia	224	18	unclear	unclear	JJ
cord-300796-rmjv56ia	224	19	.	.	.
cord-300796-rmjv56ia	225	1	Their	-PRON-	PRP$
cord-300796-rmjv56ia	225	2	transient	transient	JJ
cord-300796-rmjv56ia	225	3	appearance	appearance	NN
cord-300796-rmjv56ia	225	4	before	before	IN
cord-300796-rmjv56ia	225	5	the	the	DT
cord-300796-rmjv56ia	225	6	completion	completion	NN
cord-300796-rmjv56ia	225	7	of	of	IN
cord-300796-rmjv56ia	225	8	the	the	DT
cord-300796-rmjv56ia	225	9	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	225	10	chain	chain	NN
cord-300796-rmjv56ia	225	11	suggests	suggest	VBZ
cord-300796-rmjv56ia	225	12	that	that	IN
cord-300796-rmjv56ia	225	13	they	-PRON-	PRP
cord-300796-rmjv56ia	225	14	represent	represent	VBP
cord-300796-rmjv56ia	225	15	complexes	complex	NNS
cord-300796-rmjv56ia	225	16	of	of	IN
cord-300796-rmjv56ia	225	17	nascent	nascent	JJ
cord-300796-rmjv56ia	225	18	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	225	19	chains	chain	NNS
cord-300796-rmjv56ia	225	20	.	.	.
cord-300796-rmjv56ia	226	1	Figure	figure	NN
cord-300796-rmjv56ia	226	2	6	6	CD
cord-300796-rmjv56ia	226	3	.	.	.
cord-300796-rmjv56ia	227	1	Time	time	NN
cord-300796-rmjv56ia	227	2	course	course	NN
cord-300796-rmjv56ia	227	3	of	of	IN
cord-300796-rmjv56ia	227	4	p62	p62	NN
cord-300796-rmjv56ia	227	5	d-4	d-4	NN
cord-300796-rmjv56ia	227	6	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	227	7	.	.	.
cord-300796-rmjv56ia	228	1	Seven	seven	CD
cord-300796-rmjv56ia	228	2	translations	translation	NNS
cord-300796-rmjv56ia	228	3	in	in	IN
cord-300796-rmjv56ia	228	4	the	the	DT
cord-300796-rmjv56ia	228	5	presence	presence	NN
cord-300796-rmjv56ia	228	6	of	of	IN
cord-300796-rmjv56ia	228	7	microsomal	microsomal	NNP
cord-300796-rmjv56ia	228	8	membranes	membrane	NNS
cord-300796-rmjv56ia	228	9	were	be	VBD
cord-300796-rmjv56ia	228	10	started	start	VBN
cord-300796-rmjv56ia	228	11	in	in	IN
cord-300796-rmjv56ia	228	12	parallel	parallel	NN
cord-300796-rmjv56ia	228	13	.	.	.
cord-300796-rmjv56ia	229	1	After	after	IN
cord-300796-rmjv56ia	229	2	a	a	DT
cord-300796-rmjv56ia	229	3	3-rain	3-rain	CD
cord-300796-rmjv56ia	229	4	initial	initial	JJ
cord-300796-rmjv56ia	229	5	incubation	incubation	NN
cord-300796-rmjv56ia	229	6	at	at	IN
cord-300796-rmjv56ia	229	7	30	30	CD
cord-300796-rmjv56ia	229	8	°	°	NN
cord-300796-rmjv56ia	229	9	C	c	NN
cord-300796-rmjv56ia	229	10	,	,	,
cord-300796-rmjv56ia	229	11	ATA	ATA	NNP
cord-300796-rmjv56ia	229	12	was	be	VBD
cord-300796-rmjv56ia	229	13	added	add	VBN
cord-300796-rmjv56ia	229	14	in	in	IN
cord-300796-rmjv56ia	229	15	order	order	NN
cord-300796-rmjv56ia	229	16	to	to	TO
cord-300796-rmjv56ia	229	17	inhibit	inhibit	VB
cord-300796-rmjv56ia	229	18	further	further	JJ
cord-300796-rmjv56ia	229	19	initiation	initiation	NN
cord-300796-rmjv56ia	229	20	of	of	IN
cord-300796-rmjv56ia	229	21	chain	chain	NN
cord-300796-rmjv56ia	229	22	synthesis	synthesis	NN
cord-300796-rmjv56ia	229	23	.	.	.
cord-300796-rmjv56ia	230	1	Incubation	incubation	NN
cord-300796-rmjv56ia	230	2	was	be	VBD
cord-300796-rmjv56ia	230	3	then	then	RB
cord-300796-rmjv56ia	230	4	continued	continue	VBN
cord-300796-rmjv56ia	230	5	for	for	IN
cord-300796-rmjv56ia	230	6	35	35	CD
cord-300796-rmjv56ia	230	7	rain	rain	NN
cord-300796-rmjv56ia	230	8	.	.	.
cord-300796-rmjv56ia	231	1	At	at	IN
cord-300796-rmjv56ia	231	2	the	the	DT
cord-300796-rmjv56ia	231	3	indicated	indicate	VBN
cord-300796-rmjv56ia	231	4	time	time	NN
cord-300796-rmjv56ia	231	5	points	point	NNS
cord-300796-rmjv56ia	231	6	(	(	-LRB-
cord-300796-rmjv56ia	231	7	5	5	CD
cord-300796-rmjv56ia	231	8	,	,	,
cord-300796-rmjv56ia	231	9	10	10	CD
cord-300796-rmjv56ia	231	10	,	,	,
cord-300796-rmjv56ia	231	11	15	15	CD
cord-300796-rmjv56ia	231	12	,	,	,
cord-300796-rmjv56ia	231	13	20	20	CD
cord-300796-rmjv56ia	231	14	,	,	,
cord-300796-rmjv56ia	231	15	25	25	CD
cord-300796-rmjv56ia	231	16	,	,	,
cord-300796-rmjv56ia	231	17	30	30	CD
cord-300796-rmjv56ia	231	18	,	,	,
cord-300796-rmjv56ia	231	19	and	and	CC
cord-300796-rmjv56ia	231	20	35	35	CD
cord-300796-rmjv56ia	231	21	rain	rain	NN
cord-300796-rmjv56ia	231	22	)	)	-RRB-
cord-300796-rmjv56ia	231	23	TX-100	TX-100	NNP
cord-300796-rmjv56ia	231	24	(	(	-LRB-
cord-300796-rmjv56ia	231	25	TX	TX	NNP
cord-300796-rmjv56ia	231	26	)	)	-RRB-
cord-300796-rmjv56ia	231	27	was	be	VBD
cord-300796-rmjv56ia	231	28	added	add	VBN
cord-300796-rmjv56ia	231	29	to	to	TO
cord-300796-rmjv56ia	231	30	stop	stop	VB
cord-300796-rmjv56ia	231	31	further	further	JJ
cord-300796-rmjv56ia	231	32	chain	chain	NN
cord-300796-rmjv56ia	231	33	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	231	34	.	.	.
cord-300796-rmjv56ia	232	1	At	at	IN
cord-300796-rmjv56ia	232	2	the	the	DT
cord-300796-rmjv56ia	232	3	same	same	JJ
cord-300796-rmjv56ia	232	4	time	time	NN
cord-300796-rmjv56ia	232	5	one	one	CD
cord-300796-rmjv56ia	232	6	half	half	NN
cord-300796-rmjv56ia	232	7	of	of	IN
cord-300796-rmjv56ia	232	8	each	each	DT
cord-300796-rmjv56ia	232	9	sample	sample	NN
cord-300796-rmjv56ia	232	10	was	be	VBD
cord-300796-rmjv56ia	232	11	removed	remove	VBN
cord-300796-rmjv56ia	232	12	and	and	CC
cord-300796-rmjv56ia	232	13	put	put	VBN
cord-300796-rmjv56ia	232	14	on	on	IN
cord-300796-rmjv56ia	232	15	ice	ice	NN
cord-300796-rmjv56ia	232	16	in	in	IN
cord-300796-rmjv56ia	232	17	order	order	NN
cord-300796-rmjv56ia	232	18	to	to	TO
cord-300796-rmjv56ia	232	19	measure	measure	VB
cord-300796-rmjv56ia	232	20	the	the	DT
cord-300796-rmjv56ia	232	21	extent	extent	NN
cord-300796-rmjv56ia	232	22	of	of	IN
cord-300796-rmjv56ia	232	23	chain	chain	NN
cord-300796-rmjv56ia	232	24	elongation	elongation	NN
cord-300796-rmjv56ia	232	25	at	at	IN
cord-300796-rmjv56ia	232	26	each	each	DT
cord-300796-rmjv56ia	232	27	time	time	NN
cord-300796-rmjv56ia	232	28	point	point	NN
cord-300796-rmjv56ia	232	29	.	.	.
cord-300796-rmjv56ia	233	1	All	all	DT
cord-300796-rmjv56ia	233	2	samples	sample	NNS
cord-300796-rmjv56ia	233	3	were	be	VBD
cord-300796-rmjv56ia	233	4	analyzed	analyze	VBN
cord-300796-rmjv56ia	233	5	by	by	IN
cord-300796-rmjv56ia	233	6	SDS	SDS	NNP
cord-300796-rmjv56ia	233	7	-	-	HYPH
cord-300796-rmjv56ia	233	8	PAGE	PAGE	NNP
cord-300796-rmjv56ia	233	9	(	(	-LRB-
cord-300796-rmjv56ia	233	10	10	10	CD
cord-300796-rmjv56ia	233	11	%	%	NN
cord-300796-rmjv56ia	233	12	)	)	-RRB-
cord-300796-rmjv56ia	233	13	and	and	CC
cord-300796-rmjv56ia	233	14	autoradiography	autoradiography	NN
cord-300796-rmjv56ia	233	15	.	.	.
cord-300796-rmjv56ia	234	1	Lanes	lane	NNS
cord-300796-rmjv56ia	234	2	3	3	CD
cord-300796-rmjv56ia	234	3	-	-	SYM
cord-300796-rmjv56ia	234	4	9	9	CD
cord-300796-rmjv56ia	234	5	show	show	VBP
cord-300796-rmjv56ia	234	6	the	the	DT
cord-300796-rmjv56ia	234	7	analysis	analysis	NN
cord-300796-rmjv56ia	234	8	of	of	IN
cord-300796-rmjv56ia	234	9	the	the	DT
cord-300796-rmjv56ia	234	10	samples	sample	NNS
cord-300796-rmjv56ia	234	11	incubatedwith	incubatedwith	NN
cord-300796-rmjv56ia	234	12	TX-100	TX-100	NNP
cord-300796-rmjv56ia	234	13	and	and	CC
cord-300796-rmjv56ia	234	14	lanes	lane	NNS
cord-300796-rmjv56ia	234	15	10	10	CD
cord-300796-rmjv56ia	234	16	-	-	HYPH
cord-300796-rmjv56ia	234	17	-16	-16	NN
cord-300796-rmjv56ia	235	1	the	the	DT
cord-300796-rmjv56ia	235	2	analysis	analysis	NN
cord-300796-rmjv56ia	235	3	of	of	IN
cord-300796-rmjv56ia	235	4	the	the	DT
cord-300796-rmjv56ia	235	5	portions	portion	NNS
cord-300796-rmjv56ia	235	6	put	put	VBN
cord-300796-rmjv56ia	235	7	on	on	IN
cord-300796-rmjv56ia	235	8	ice	ice	NN
cord-300796-rmjv56ia	235	9	at	at	IN
cord-300796-rmjv56ia	235	10	the	the	DT
cord-300796-rmjv56ia	235	11	different	different	JJ
cord-300796-rmjv56ia	235	12	time	time	NN
cord-300796-rmjv56ia	235	13	points	point	NNS
cord-300796-rmjv56ia	235	14	.	.	.
cord-300796-rmjv56ia	236	1	The	the	DT
cord-300796-rmjv56ia	236	2	complete	complete	JJ
cord-300796-rmjv56ia	236	3	sequence	sequence	NN
cord-300796-rmjv56ia	236	4	of	of	IN
cord-300796-rmjv56ia	236	5	treatments	treatment	NNS
cord-300796-rmjv56ia	236	6	for	for	IN
cord-300796-rmjv56ia	236	7	each	each	DT
cord-300796-rmjv56ia	236	8	sample	sample	NN
cord-300796-rmjv56ia	236	9	is	be	VBZ
cord-300796-rmjv56ia	236	10	indicated	indicate	VBN
cord-300796-rmjv56ia	236	11	by	by	IN
cord-300796-rmjv56ia	236	12	the	the	DT
cord-300796-rmjv56ia	236	13	labeling	labeling	NN
cord-300796-rmjv56ia	236	14	in	in	IN
cord-300796-rmjv56ia	236	15	each	each	DT
cord-300796-rmjv56ia	236	16	lane	lane	NN
cord-300796-rmjv56ia	236	17	(	(	-LRB-
cord-300796-rmjv56ia	236	18	upper	upper	JJ
cord-300796-rmjv56ia	236	19	row	row	NN
cord-300796-rmjv56ia	236	20	of	of	IN
cord-300796-rmjv56ia	236	21	time	time	NN
cord-300796-rmjv56ia	236	22	points	point	NNS
cord-300796-rmjv56ia	236	23	indicate	indicate	VBP
cord-300796-rmjv56ia	236	24	TX-100	TX-100	NNP
cord-300796-rmjv56ia	236	25	addition	addition	NN
cord-300796-rmjv56ia	236	26	and	and	CC
cord-300796-rmjv56ia	236	27	cooling	cool	VBG
cord-300796-rmjv56ia	236	28	on	on	IN
cord-300796-rmjv56ia	236	29	ice	ice	NN
cord-300796-rmjv56ia	236	30	,	,	,
cord-300796-rmjv56ia	236	31	respectively	respectively	RB
cord-300796-rmjv56ia	236	32	;	;	:
cord-300796-rmjv56ia	236	33	lower	low	JJR
cord-300796-rmjv56ia	236	34	row	row	NN
cord-300796-rmjv56ia	236	35	of	of	IN
cord-300796-rmjv56ia	236	36	timepoints	timepoint	NNS
cord-300796-rmjv56ia	236	37	indicate	indicate	VBP
cord-300796-rmjv56ia	236	38	incubation	incubation	NN
cord-300796-rmjv56ia	236	39	in	in	IN
cord-300796-rmjv56ia	236	40	the	the	DT
cord-300796-rmjv56ia	236	41	presence	presence	NN
cord-300796-rmjv56ia	236	42	of	of	IN
cord-300796-rmjv56ia	236	43	TX-100	TX-100	NNP
cord-300796-rmjv56ia	236	44	)	)	-RRB-
cord-300796-rmjv56ia	236	45	.	.	.
cord-300796-rmjv56ia	237	1	Lanes	lane	NNS
cord-300796-rmjv56ia	237	2	I	-PRON-	PRP
cord-300796-rmjv56ia	237	3	and	and	CC
cord-300796-rmjv56ia	237	4	2	2	CD
cord-300796-rmjv56ia	237	5	represent	represent	VBP
cord-300796-rmjv56ia	237	6	controls	control	NNS
cord-300796-rmjv56ia	237	7	.	.	.
cord-300796-rmjv56ia	238	1	In	in	IN
cord-300796-rmjv56ia	238	2	the	the	DT
cord-300796-rmjv56ia	238	3	experiment	experiment	NN
cord-300796-rmjv56ia	238	4	shown	show	VBN
cord-300796-rmjv56ia	238	5	in	in	IN
cord-300796-rmjv56ia	238	6	lane	lane	NN
cord-300796-rmjv56ia	238	7	I	I	NNP
cord-300796-rmjv56ia	238	8	,	,	,
cord-300796-rmjv56ia	238	9	ATA	ATA	NNP
cord-300796-rmjv56ia	238	10	was	be	VBD
cord-300796-rmjv56ia	238	11	added	add	VBN
cord-300796-rmjv56ia	238	12	before	before	IN
cord-300796-rmjv56ia	238	13	starting	start	VBG
cord-300796-rmjv56ia	238	14	a	a	DT
cord-300796-rmjv56ia	238	15	40-rain	40-rain	CD
cord-300796-rmjv56ia	238	16	membrane	membrane	NN
cord-300796-rmjv56ia	238	17	-	-	HYPH
cord-300796-rmjv56ia	238	18	supplemented	supplement	VBN
cord-300796-rmjv56ia	238	19	translation	translation	NN
cord-300796-rmjv56ia	238	20	.	.	.
cord-300796-rmjv56ia	239	1	In	in	IN
cord-300796-rmjv56ia	239	2	the	the	DT
cord-300796-rmjv56ia	239	3	experiment	experiment	NN
cord-300796-rmjv56ia	239	4	shown	show	VBN
cord-300796-rmjv56ia	239	5	in	in	IN
cord-300796-rmjv56ia	239	6	lane	lane	NN
cord-300796-rmjv56ia	239	7	2	2	CD
cord-300796-rmjv56ia	239	8	a	a	DT
cord-300796-rmjv56ia	239	9	translation	translation	NN
cord-300796-rmjv56ia	239	10	with	with	IN
cord-300796-rmjv56ia	239	11	membranes	membrane	NNS
cord-300796-rmjv56ia	239	12	was	be	VBD
cord-300796-rmjv56ia	239	13	allowed	allow	VBN
cord-300796-rmjv56ia	239	14	to	to	TO
cord-300796-rmjv56ia	239	15	proceed	proceed	VB
cord-300796-rmjv56ia	239	16	for	for	IN
cord-300796-rmjv56ia	239	17	40	40	CD
cord-300796-rmjv56ia	239	18	min	min	NN
cord-300796-rmjv56ia	239	19	.	.	.
cord-300796-rmjv56ia	240	1	ATA	ATA	NNP
cord-300796-rmjv56ia	240	2	was	be	VBD
cord-300796-rmjv56ia	240	3	added	add	VBN
cord-300796-rmjv56ia	240	4	as	as	IN
cord-300796-rmjv56ia	240	5	in	in	IN
cord-300796-rmjv56ia	240	6	the	the	DT
cord-300796-rmjv56ia	240	7	time	time	NN
cord-300796-rmjv56ia	240	8	course	course	NN
cord-300796-rmjv56ia	240	9	samples	sample	NNS
cord-300796-rmjv56ia	240	10	but	but	CC
cord-300796-rmjv56ia	240	11	TX-100	TX-100	NNP
cord-300796-rmjv56ia	240	12	was	be	VBD
cord-300796-rmjv56ia	240	13	omitted	omit	VBN
cord-300796-rmjv56ia	240	14	.	.	.
cord-300796-rmjv56ia	241	1	The	the	DT
cord-300796-rmjv56ia	241	2	C	C	NNP
cord-300796-rmjv56ia	241	3	protein	protein	NN
cord-300796-rmjv56ia	241	4	,	,	,
cord-300796-rmjv56ia	241	5	the	the	DT
cord-300796-rmjv56ia	241	6	unglycosylated	unglycosylated	JJ
cord-300796-rmjv56ia	241	7	(	(	-LRB-
cord-300796-rmjv56ia	241	8	p62	p62	NN
cord-300796-rmjv56ia	241	9	)	)	-RRB-
cord-300796-rmjv56ia	241	10	and	and	CC
cord-300796-rmjv56ia	241	11	the	the	DT
cord-300796-rmjv56ia	241	12	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	241	13	(	(	-LRB-
cord-300796-rmjv56ia	241	14	gp62	gp62	NNP
cord-300796-rmjv56ia	241	15	)	)	-RRB-
cord-300796-rmjv56ia	241	16	forms	form	NNS
cord-300796-rmjv56ia	241	17	of	of	IN
cord-300796-rmjv56ia	241	18	p62	p62	NN
cord-300796-rmjv56ia	241	19	d-4	d-4	NN
cord-300796-rmjv56ia	241	20	are	be	VBP
cord-300796-rmjv56ia	241	21	labeled	label	VBN
cord-300796-rmjv56ia	241	22	at	at	IN
cord-300796-rmjv56ia	241	23	right	right	RB
cord-300796-rmjv56ia	241	24	in	in	IN
cord-300796-rmjv56ia	241	25	the	the	DT
cord-300796-rmjv56ia	241	26	figure	figure	NN
cord-300796-rmjv56ia	241	27	.	.	.
cord-300796-rmjv56ia	242	1	Arrowheads	arrowhead	NNS
cord-300796-rmjv56ia	242	2	at	at	IN
cord-300796-rmjv56ia	242	3	left	left	JJ
cord-300796-rmjv56ia	242	4	indicate	indicate	VBP
cord-300796-rmjv56ia	242	5	(	(	-LRB-
cord-300796-rmjv56ia	242	6	from	from	IN
cord-300796-rmjv56ia	242	7	above	above	RB
cord-300796-rmjv56ia	242	8	)	)	-RRB-
cord-300796-rmjv56ia	243	1	the	the	DT
cord-300796-rmjv56ia	243	2	migration	migration	NN
cord-300796-rmjv56ia	243	3	of	of	IN
cord-300796-rmjv56ia	243	4	the	the	DT
cord-300796-rmjv56ia	243	5	53-kD	53-kd	CD
cord-300796-rmjv56ia	243	6	IgG	IgG	NNP
cord-300796-rmjv56ia	243	7	heavy	heavy	JJ
cord-300796-rmjv56ia	243	8	chain	chain	NN
cord-300796-rmjv56ia	243	9	,	,	,
cord-300796-rmjv56ia	243	10	the	the	DT
cord-300796-rmjv56ia	243	11	46-kD	46-kd	CD
cord-300796-rmjv56ia	243	12	ovalbumin	ovalbumin	NNP
cord-300796-rmjv56ia	243	13	,	,	,
cord-300796-rmjv56ia	243	14	and	and	CC
cord-300796-rmjv56ia	243	15	the	the	DT
cord-300796-rmjv56ia	243	16	30-kD	30-kd	CD
cord-300796-rmjv56ia	243	17	carbonic	carbonic	JJ
cord-300796-rmjv56ia	243	18	anhydrase	anhydrase	NN
cord-300796-rmjv56ia	243	19	.	.	.
cord-300796-rmjv56ia	244	1	Note	note	VB
cord-300796-rmjv56ia	244	2	that	that	IN
cord-300796-rmjv56ia	244	3	somewhat	somewhat	RB
cord-300796-rmjv56ia	244	4	different	different	JJ
cord-300796-rmjv56ia	244	5	amounts	amount	NNS
cord-300796-rmjv56ia	244	6	of	of	IN
cord-300796-rmjv56ia	244	7	translation	translation	NN
cord-300796-rmjv56ia	244	8	mixtures	mixture	NNS
cord-300796-rmjv56ia	244	9	have	have	VBP
cord-300796-rmjv56ia	244	10	been	be	VBN
cord-300796-rmjv56ia	244	11	analyzed	analyze	VBN
cord-300796-rmjv56ia	244	12	in	in	IN
cord-300796-rmjv56ia	244	13	the	the	DT
cord-300796-rmjv56ia	244	14	various	various	JJ
cord-300796-rmjv56ia	244	15	lanes	lane	NNS
cord-300796-rmjv56ia	244	16	(	(	-LRB-
cord-300796-rmjv56ia	244	17	compare	compare	VB
cord-300796-rmjv56ia	244	18	intensities	intensity	NNS
cord-300796-rmjv56ia	244	19	of	of	IN
cord-300796-rmjv56ia	244	20	C	C	NNP
cord-300796-rmjv56ia	244	21	and	and	CC
cord-300796-rmjv56ia	244	22	C	c	NN
cord-300796-rmjv56ia	244	23	-	-	HYPH
cord-300796-rmjv56ia	244	24	derived	derive	VBN
cord-300796-rmjv56ia	244	25	bands	band	NNS
cord-300796-rmjv56ia	244	26	)	)	-RRB-
cord-300796-rmjv56ia	244	27	.	.	.
cord-300796-rmjv56ia	245	1	1.5-rain	1.5-rain	CD
cord-300796-rmjv56ia	245	2	preincubation	preincubation	NN
cord-300796-rmjv56ia	245	3	without	without	IN
cord-300796-rmjv56ia	245	4	ATA	ATA	NNP
cord-300796-rmjv56ia	245	5	then	then	RB
cord-300796-rmjv56ia	245	6	the	the	DT
cord-300796-rmjv56ia	245	7	total	total	JJ
cord-300796-rmjv56ia	245	8	time	time	NN
cord-300796-rmjv56ia	245	9	for	for	IN
cord-300796-rmjv56ia	245	10	chain	chain	NN
cord-300796-rmjv56ia	245	11	synthesis	synthesis	NN
cord-300796-rmjv56ia	245	12	is	be	VBZ
cord-300796-rmjv56ia	245	13	,	,	,
cord-300796-rmjv56ia	245	14	~3.75	~3.75	NFP
cord-300796-rmjv56ia	245	15	rain	rain	NN
cord-300796-rmjv56ia	245	16	(	(	-LRB-
cord-300796-rmjv56ia	245	17	3	3	CD
cord-300796-rmjv56ia	245	18	+	+	SYM
cord-300796-rmjv56ia	245	19	0.75	0.75	CD
cord-300796-rmjv56ia	245	20	rain	rain	NN
cord-300796-rmjv56ia	245	21	)	)	-RRB-
cord-300796-rmjv56ia	245	22	.	.	.
cord-300796-rmjv56ia	246	1	This	this	DT
cord-300796-rmjv56ia	246	2	corresponds	correspond	VBZ
cord-300796-rmjv56ia	246	3	to	to	IN
cord-300796-rmjv56ia	246	4	a	a	DT
cord-300796-rmjv56ia	246	5	mean	mean	JJ
cord-300796-rmjv56ia	246	6	translation	translation	NN
cord-300796-rmjv56ia	246	7	rate	rate	NN
cord-300796-rmjv56ia	246	8	of	of	IN
cord-300796-rmjv56ia	246	9	52.5	52.5	CD
cord-300796-rmjv56ia	246	10	peptide	peptide	NN
cord-300796-rmjv56ia	246	11	bonds	bond	NNS
cord-300796-rmjv56ia	246	12	per	per	IN
cord-300796-rmjv56ia	246	13	rain	rain	NN
cord-300796-rmjv56ia	246	14	.	.	.
cord-300796-rmjv56ia	247	1	Lanes	lane	NNS
cord-300796-rmjv56ia	247	2	11	11	CD
cord-300796-rmjv56ia	247	3	-	-	SYM
cord-300796-rmjv56ia	247	4	20	20	CD
cord-300796-rmjv56ia	247	5	show	show	NN
cord-300796-rmjv56ia	247	6	that	that	IN
cord-300796-rmjv56ia	247	7	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	247	8	chains	chain	NNS
cord-300796-rmjv56ia	247	9	appear	appear	VBP
cord-300796-rmjv56ia	247	10	in	in	IN
cord-300796-rmjv56ia	247	11	all	all	PDT
cord-300796-rmjv56ia	247	12	those	those	DT
cord-300796-rmjv56ia	247	13	samples	sample	NNS
cord-300796-rmjv56ia	247	14	that	that	WDT
cord-300796-rmjv56ia	247	15	have	have	VBP
cord-300796-rmjv56ia	247	16	had	have	VBN
cord-300796-rmjv56ia	247	17	the	the	DT
cord-300796-rmjv56ia	247	18	membranes	membrane	NNS
cord-300796-rmjv56ia	247	19	intact	intact	JJ
cord-300796-rmjv56ia	247	20	for	for	IN
cord-300796-rmjv56ia	247	21	1.5	1.5	CD
cord-300796-rmjv56ia	247	22	min	min	NNS
cord-300796-rmjv56ia	247	23	or	or	CC
cord-300796-rmjv56ia	247	24	more	more	JJR
cord-300796-rmjv56ia	247	25	after	after	IN
cord-300796-rmjv56ia	247	26	ATA	ATA	NNP
cord-300796-rmjv56ia	247	27	addition	addition	NN
cord-300796-rmjv56ia	247	28	.	.	.
cord-300796-rmjv56ia	248	1	This	this	DT
cord-300796-rmjv56ia	248	2	means	mean	VBZ
cord-300796-rmjv56ia	248	3	that	that	IN
cord-300796-rmjv56ia	248	4	p62dhfr	p62dhfr	NN
cord-300796-rmjv56ia	248	5	chains	chain	NNS
cord-300796-rmjv56ia	248	6	that	that	WDT
cord-300796-rmjv56ia	248	7	have	have	VBP
cord-300796-rmjv56ia	248	8	been	be	VBN
cord-300796-rmjv56ia	248	9	elongated	elongated	JJ
cord-300796-rmjv56ia	248	10	for	for	IN
cord-300796-rmjv56ia	248	11	'	'	NNS
cord-300796-rmjv56ia	248	12	~2.25	~2.25	CD
cord-300796-rmjv56ia	248	13	rain	rain	NN
cord-300796-rmjv56ia	248	14	(	(	-LRB-
cord-300796-rmjv56ia	248	15	1.5	1.5	CD
cord-300796-rmjv56ia	248	16	min	min	NN
cord-300796-rmjv56ia	248	17	incubation	incubation	NN
cord-300796-rmjv56ia	248	18	after	after	IN
cord-300796-rmjv56ia	248	19	and	and	CC
cord-300796-rmjv56ia	248	20	0.75	0.75	CD
cord-300796-rmjv56ia	248	21	min	min	NN
cord-300796-rmjv56ia	248	22	before	before	IN
cord-300796-rmjv56ia	248	23	ATA	ATA	NNP
cord-300796-rmjv56ia	248	24	addition	addition	NN
cord-300796-rmjv56ia	248	25	)	)	-RRB-
cord-300796-rmjv56ia	248	26	,	,	,
cord-300796-rmjv56ia	248	27	to	to	IN
cord-300796-rmjv56ia	248	28	the	the	DT
cord-300796-rmjv56ia	248	29	length	length	NN
cord-300796-rmjv56ia	248	30	of	of	IN
cord-300796-rmjv56ia	248	31	,	,	,
cord-300796-rmjv56ia	248	32	'	'	''
cord-300796-rmjv56ia	248	33	~118	~118	NFP
cord-300796-rmjv56ia	248	34	residues	residue	NNS
cord-300796-rmjv56ia	248	35	already	already	RB
cord-300796-rmjv56ia	248	36	carry	carry	VBP
cord-300796-rmjv56ia	248	37	a	a	DT
cord-300796-rmjv56ia	248	38	sugar	sugar	NN
cord-300796-rmjv56ia	248	39	unit	unit	NN
cord-300796-rmjv56ia	248	40	at	at	IN
cord-300796-rmjv56ia	248	41	Asn13	Asn13	NNP
cord-300796-rmjv56ia	248	42	.	.	.
cord-300796-rmjv56ia	249	1	As	as	IN
cord-300796-rmjv56ia	249	2	~60	~60	CD
cord-300796-rmjv56ia	249	3	residues	residue	NNS
cord-300796-rmjv56ia	249	4	of	of	IN
cord-300796-rmjv56ia	249	5	the	the	DT
cord-300796-rmjv56ia	249	6	nascent	nascent	JJ
cord-300796-rmjv56ia	249	7	chain	chain	NN
cord-300796-rmjv56ia	249	8	are	be	VBP
cord-300796-rmjv56ia	249	9	required	require	VBN
cord-300796-rmjv56ia	249	10	to	to	TO
cord-300796-rmjv56ia	249	11	span	span	VB
cord-300796-rmjv56ia	249	12	the	the	DT
cord-300796-rmjv56ia	249	13	ribosome	ribosome	NN
cord-300796-rmjv56ia	249	14	and	and	CC
cord-300796-rmjv56ia	249	15	the	the	DT
cord-300796-rmjv56ia	249	16	lipid	lipid	NN
cord-300796-rmjv56ia	249	17	bilayer	bilayer	NN
cord-300796-rmjv56ia	249	18	we	-PRON-	PRP
cord-300796-rmjv56ia	249	19	conclude	conclude	VBP
cord-300796-rmjv56ia	249	20	that	that	IN
cord-300796-rmjv56ia	249	21	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	249	22	occurs	occur	VBZ
cord-300796-rmjv56ia	249	23	when	when	WRB
cord-300796-rmjv56ia	249	24	the	the	DT
cord-300796-rmjv56ia	249	25	first	first	JJ
cord-300796-rmjv56ia	249	26	50	50	CD
cord-300796-rmjv56ia	249	27	-	-	SYM
cord-300796-rmjv56ia	249	28	60	60	CD
cord-300796-rmjv56ia	249	29	residues	residue	NNS
cord-300796-rmjv56ia	249	30	of	of	IN
cord-300796-rmjv56ia	249	31	p62-dhfr	p62-dhfr	NN
cord-300796-rmjv56ia	249	32	appear	appear	VBP
cord-300796-rmjv56ia	249	33	within	within	IN
cord-300796-rmjv56ia	249	34	the	the	DT
cord-300796-rmjv56ia	249	35	lumen	lumen	NN
cord-300796-rmjv56ia	249	36	of	of	IN
cord-300796-rmjv56ia	249	37	the	the	DT
cord-300796-rmjv56ia	249	38	ER	ER	NNP
cord-300796-rmjv56ia	249	39	(	(	-LRB-
cord-300796-rmjv56ia	249	40	Malkin	Malkin	NNP
cord-300796-rmjv56ia	249	41	and	and	CC
cord-300796-rmjv56ia	249	42	Rich	Rich	NNP
cord-300796-rmjv56ia	249	43	,	,	,
cord-300796-rmjv56ia	249	44	1967	1967	CD
cord-300796-rmjv56ia	249	45	;	;	:
cord-300796-rmjv56ia	249	46	Blobel	Blobel	NNP
cord-300796-rmjv56ia	249	47	and	and	CC
cord-300796-rmjv56ia	249	48	Sabatini	Sabatini	NNP
cord-300796-rmjv56ia	249	49	,	,	,
cord-300796-rmjv56ia	249	50	1970	1970	CD
cord-300796-rmjv56ia	249	51	;	;	.
cord-300796-rmjv56ia	250	1	Bergman	Bergman	NNP
cord-300796-rmjv56ia	250	2	and	and	CC
cord-300796-rmjv56ia	250	3	Kuehl	Kuehl	NNP
cord-300796-rmjv56ia	250	4	,	,	,
cord-300796-rmjv56ia	250	5	1977	1977	CD
cord-300796-rmjv56ia	250	6	;	;	:
cord-300796-rmjv56ia	250	7	Smith	Smith	NNP
cord-300796-rmjv56ia	250	8	et	et	NNP
cord-300796-rmjv56ia	250	9	al	al	NNP
cord-300796-rmjv56ia	250	10	.	.	NNP
cord-300796-rmjv56ia	250	11	,	,	,
cord-300796-rmjv56ia	250	12	1978	1978	CD
cord-300796-rmjv56ia	250	13	;	;	:
cord-300796-rmjv56ia	250	14	Glabe	Glabe	NNP
cord-300796-rmjv56ia	250	15	et	et	NNP
cord-300796-rmjv56ia	250	16	al	al	NNP
cord-300796-rmjv56ia	250	17	.	.	NNP
cord-300796-rmjv56ia	250	18	,	,	,
cord-300796-rmjv56ia	250	19	1980	1980	CD
cord-300796-rmjv56ia	250	20	;	;	:
cord-300796-rmjv56ia	250	21	Randall	Randall	NNP
cord-300796-rmjv56ia	250	22	,	,	,
cord-300796-rmjv56ia	250	23	1983	1983	CD
cord-300796-rmjv56ia	250	24	)	)	-RRB-
cord-300796-rmjv56ia	250	25	.	.	.
cord-300796-rmjv56ia	251	1	We	-PRON-	PRP
cord-300796-rmjv56ia	251	2	also	also	RB
cord-300796-rmjv56ia	251	3	studied	study	VBD
cord-300796-rmjv56ia	251	4	the	the	DT
cord-300796-rmjv56ia	251	5	timing	timing	NN
cord-300796-rmjv56ia	251	6	of	of	IN
cord-300796-rmjv56ia	251	7	the	the	DT
cord-300796-rmjv56ia	251	8	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	251	9	of	of	IN
cord-300796-rmjv56ia	251	10	Asn~3	Asn~3	NNP
cord-300796-rmjv56ia	251	11	in	in	IN
cord-300796-rmjv56ia	251	12	its	-PRON-	PRP$
cord-300796-rmjv56ia	251	13	normal	normal	JJ
cord-300796-rmjv56ia	251	14	background	background	NN
cord-300796-rmjv56ia	251	15	,	,	,
cord-300796-rmjv56ia	251	16	i.e.	i.e.	FW
cord-300796-rmjv56ia	251	17	,	,	,
cord-300796-rmjv56ia	251	18	during	during	IN
cord-300796-rmjv56ia	251	19	p62	p62	NN
cord-300796-rmjv56ia	251	20	chain	chain	NN
cord-300796-rmjv56ia	251	21	synthesis	synthesis	NN
cord-300796-rmjv56ia	251	22	.	.	.
cord-300796-rmjv56ia	252	1	For	for	IN
cord-300796-rmjv56ia	252	2	this	this	DT
cord-300796-rmjv56ia	252	3	experiment	experiment	NN
cord-300796-rmjv56ia	252	4	we	-PRON-	PRP
cord-300796-rmjv56ia	252	5	used	use	VBD
cord-300796-rmjv56ia	252	6	the	the	DT
cord-300796-rmjv56ia	252	7	pGEM	pgem	NN
cord-300796-rmjv56ia	252	8	SFVd-4	sfvd-4	VB
cord-300796-rmjv56ia	252	9	construct	construct	NN
cord-300796-rmjv56ia	252	10	.	.	.
cord-300796-rmjv56ia	253	1	This	this	DT
cord-300796-rmjv56ia	253	2	encodes	encode	VBZ
cord-300796-rmjv56ia	253	3	the	the	DT
cord-300796-rmjv56ia	253	4	C	C	NNP
cord-300796-rmjv56ia	253	5	and	and	CC
cord-300796-rmjv56ia	253	6	the	the	DT
cord-300796-rmjv56ia	253	7	p62	p62	NN
cord-300796-rmjv56ia	253	8	membrane	membrane	NN
cord-300796-rmjv56ia	253	9	protein	protein	NN
cord-300796-rmjv56ia	253	10	variant	variant	NN
cord-300796-rmjv56ia	253	11	,	,	,
cord-300796-rmjv56ia	253	12	p62	p62	NN
cord-300796-rmjv56ia	253	13	d-4	d-4	NN
cord-300796-rmjv56ia	253	14	,	,	,
cord-300796-rmjv56ia	253	15	in	in	IN
cord-300796-rmjv56ia	253	16	which	which	WDT
cord-300796-rmjv56ia	253	17	a	a	DT
cord-300796-rmjv56ia	253	18	few	few	JJ
cord-300796-rmjv56ia	253	19	residues	residue	NNS
cord-300796-rmjv56ia	253	20	of	of	IN
cord-300796-rmjv56ia	253	21	the	the	DT
cord-300796-rmjv56ia	253	22	cytoplasmic	cytoplasmic	JJ
cord-300796-rmjv56ia	253	23	protein	protein	NN
cord-300796-rmjv56ia	253	24	domain	domain	NN
cord-300796-rmjv56ia	253	25	have	have	VBP
cord-300796-rmjv56ia	253	26	been	be	VBN
cord-300796-rmjv56ia	253	27	exchanged	exchange	VBN
cord-300796-rmjv56ia	253	28	as	as	IN
cord-300796-rmjv56ia	253	29	compared	compare	VBN
cord-300796-rmjv56ia	253	30	to	to	IN
cord-300796-rmjv56ia	253	31	the	the	DT
cord-300796-rmjv56ia	253	32	wild	wild	JJ
cord-300796-rmjv56ia	253	33	type	type	NN
cord-300796-rmjv56ia	253	34	sequence	sequence	NN
cord-300796-rmjv56ia	253	35	(	(	-LRB-
cord-300796-rmjv56ia	253	36	see	see	VB
cord-300796-rmjv56ia	253	37	Materials	material	NNS
cord-300796-rmjv56ia	253	38	and	and	CC
cord-300796-rmjv56ia	253	39	Methods	Methods	NNPS
cord-300796-rmjv56ia	253	40	and	and	CC
cord-300796-rmjv56ia	253	41	Fig	Fig	NNP
cord-300796-rmjv56ia	253	42	,	,	,
cord-300796-rmjv56ia	253	43	1	1	CD
cord-300796-rmjv56ia	253	44	)	)	-RRB-
cord-300796-rmjv56ia	253	45	.	.	.
cord-300796-rmjv56ia	254	1	Fig	Fig	NNP
cord-300796-rmjv56ia	254	2	.	.	.
cord-300796-rmjv56ia	255	1	6	6	CD
cord-300796-rmjv56ia	255	2	,	,	,
cord-300796-rmjv56ia	255	3	lane	lane	NN
cord-300796-rmjv56ia	255	4	2	2	CD
cord-300796-rmjv56ia	255	5	,	,	,
cord-300796-rmjv56ia	255	6	shows	show	VBZ
cord-300796-rmjv56ia	255	7	that	that	IN
cord-300796-rmjv56ia	255	8	RNA	RNA	NNP
cord-300796-rmjv56ia	255	9	,	,	,
cord-300796-rmjv56ia	255	10	which	which	WDT
cord-300796-rmjv56ia	255	11	has	have	VBZ
cord-300796-rmjv56ia	255	12	been	be	VBN
cord-300796-rmjv56ia	255	13	transcribed	transcribe	VBN
cord-300796-rmjv56ia	255	14	from	from	IN
cord-300796-rmjv56ia	255	15	this	this	DT
cord-300796-rmjv56ia	255	16	construct	construct	NN
cord-300796-rmjv56ia	255	17	,	,	,
cord-300796-rmjv56ia	255	18	directs	direct	VBZ
cord-300796-rmjv56ia	255	19	the	the	DT
cord-300796-rmjv56ia	255	20	synthesis	synthesis	NN
cord-300796-rmjv56ia	255	21	of	of	IN
cord-300796-rmjv56ia	255	22	C	C	NNP
cord-300796-rmjv56ia	255	23	and	and	CC
cord-300796-rmjv56ia	255	24	p62	p62	NN
cord-300796-rmjv56ia	255	25	d-4	d-4	NN
cord-300796-rmjv56ia	255	26	chains	chain	NNS
cord-300796-rmjv56ia	255	27	.	.	.
cord-300796-rmjv56ia	256	1	The	the	DT
cord-300796-rmjv56ia	256	2	protein	protein	NN
cord-300796-rmjv56ia	256	3	has	have	VBZ
cord-300796-rmjv56ia	256	4	catalyzed	catalyze	VBN
cord-300796-rmjv56ia	256	5	correct	correct	JJ
cord-300796-rmjv56ia	256	6	C	c	NN
cord-300796-rmjv56ia	256	7	-	-	HYPH
cord-300796-rmjv56ia	256	8	p62	p62	NN
cord-300796-rmjv56ia	256	9	cleavage	cleavage	NN
cord-300796-rmjv56ia	256	10	and	and	CC
cord-300796-rmjv56ia	256	11	the	the	DT
cord-300796-rmjv56ia	256	12	p62	p62	NN
cord-300796-rmjv56ia	256	13	signal	signal	NN
cord-300796-rmjv56ia	256	14	sequence	sequence	NN
cord-300796-rmjv56ia	256	15	has	have	VBZ
cord-300796-rmjv56ia	256	16	catalyzed	catalyze	VBN
cord-300796-rmjv56ia	256	17	the	the	DT
cord-300796-rmjv56ia	256	18	insertion	insertion	NN
cord-300796-rmjv56ia	256	19	of	of	IN
cord-300796-rmjv56ia	256	20	about	about	IN
cord-300796-rmjv56ia	256	21	half	half	NN
cord-300796-rmjv56ia	256	22	of	of	IN
cord-300796-rmjv56ia	256	23	the	the	DT
cord-300796-rmjv56ia	256	24	p62	p62	NN
cord-300796-rmjv56ia	256	25	d-4	d-4	NN
cord-300796-rmjv56ia	256	26	chains	chain	NNS
cord-300796-rmjv56ia	256	27	across	across	IN
cord-300796-rmjv56ia	256	28	the	the	DT
cord-300796-rmjv56ia	256	29	added	add	VBN
cord-300796-rmjv56ia	256	30	microsomal	microsomal	NNP
cord-300796-rmjv56ia	256	31	membranes	membrane	NNS
cord-300796-rmjv56ia	256	32	.	.	.
cord-300796-rmjv56ia	257	1	These	these	DT
cord-300796-rmjv56ia	257	2	migrate	migrate	VBP
cord-300796-rmjv56ia	257	3	as	as	IN
cord-300796-rmjv56ia	257	4	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	257	5	60	60	CD
cord-300796-rmjv56ia	257	6	-	-	SYM
cord-300796-rmjv56ia	257	7	58	58	CD
cord-300796-rmjv56ia	257	8	kD	kd	JJ
cord-300796-rmjv56ia	257	9	proteins	protein	NNS
cord-300796-rmjv56ia	257	10	in	in	IN
cord-300796-rmjv56ia	257	11	contrast	contrast	NN
cord-300796-rmjv56ia	257	12	to	to	IN
cord-300796-rmjv56ia	257	13	the	the	DT
cord-300796-rmjv56ia	257	14	noninserted	noninserted	JJ
cord-300796-rmjv56ia	257	15	molecules	molecule	NNS
cord-300796-rmjv56ia	257	16	which	which	WDT
cord-300796-rmjv56ia	257	17	have	have	VBP
cord-300796-rmjv56ia	257	18	an	an	DT
cord-300796-rmjv56ia	257	19	apparent	apparent	JJ
cord-300796-rmjv56ia	257	20	molecular	molecular	JJ
cord-300796-rmjv56ia	257	21	mass	mass	NN
cord-300796-rmjv56ia	257	22	of	of	IN
cord-300796-rmjv56ia	257	23	'	'	''
cord-300796-rmjv56ia	257	24	x,52	x,52	.
cord-300796-rmjv56ia	258	1	kD.	kD.	NNP
cord-300796-rmjv56ia	259	1	The	the	DT
cord-300796-rmjv56ia	259	2	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	259	3	and	and	CC
cord-300796-rmjv56ia	259	4	translocated	translocated	JJ
cord-300796-rmjv56ia	259	5	nature	nature	NN
cord-300796-rmjv56ia	259	6	of	of	IN
cord-300796-rmjv56ia	259	7	the	the	DT
cord-300796-rmjv56ia	259	8	60	60	CD
cord-300796-rmjv56ia	259	9	-	-	SYM
cord-300796-rmjv56ia	259	10	58	58	CD
cord-300796-rmjv56ia	259	11	kD	kd	JJ
cord-300796-rmjv56ia	259	12	material	material	NN
cord-300796-rmjv56ia	259	13	was	be	VBD
cord-300796-rmjv56ia	259	14	clearly	clearly	RB
cord-300796-rmjv56ia	259	15	demon	demon	NN
cord-300796-rmjv56ia	259	16	-	-	HYPH
cord-300796-rmjv56ia	259	17	strated	strate	VBN
cord-300796-rmjv56ia	259	18	in	in	IN
cord-300796-rmjv56ia	259	19	experiments	experiment	NNS
cord-300796-rmjv56ia	259	20	similar	similar	JJ
cord-300796-rmjv56ia	259	21	to	to	IN
cord-300796-rmjv56ia	259	22	those	those	DT
cord-300796-rmjv56ia	259	23	described	describe	VBN
cord-300796-rmjv56ia	259	24	above	above	RB
cord-300796-rmjv56ia	259	25	for	for	IN
cord-300796-rmjv56ia	259	26	the	the	DT
cord-300796-rmjv56ia	259	27	p62-dhfr	p62-dhfr	JJ
cord-300796-rmjv56ia	259	28	hybrid	hybrid	NN
cord-300796-rmjv56ia	259	29	molecule	molecule	NN
cord-300796-rmjv56ia	259	30	(	(	-LRB-
cord-300796-rmjv56ia	259	31	not	not	RB
cord-300796-rmjv56ia	259	32	shown	show	VBN
cord-300796-rmjv56ia	259	33	)	)	-RRB-
cord-300796-rmjv56ia	259	34	.	.	.
cord-300796-rmjv56ia	260	1	Altogether	altogether	RB
cord-300796-rmjv56ia	260	2	there	there	EX
cord-300796-rmjv56ia	260	3	are	be	VBP
cord-300796-rmjv56ia	260	4	four	four	CD
cord-300796-rmjv56ia	260	5	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	260	6	sites	site	NNS
cord-300796-rmjv56ia	260	7	within	within	IN
cord-300796-rmjv56ia	260	8	the	the	DT
cord-300796-rmjv56ia	260	9	p62	p62	NN
cord-300796-rmjv56ia	260	10	d-4	d-4	NN
cord-300796-rmjv56ia	260	11	sequence	sequence	NN
cord-300796-rmjv56ia	260	12	.	.	.
cord-300796-rmjv56ia	261	1	These	these	DT
cord-300796-rmjv56ia	261	2	correspond	correspond	VBP
cord-300796-rmjv56ia	261	3	to	to	IN
cord-300796-rmjv56ia	261	4	Asn	Asn	NNP
cord-300796-rmjv56ia	261	5	residues	residue	NNS
cord-300796-rmjv56ia	261	6	at	at	IN
cord-300796-rmjv56ia	261	7	positions	position	NNS
cord-300796-rmjv56ia	261	8	13	13	CD
cord-300796-rmjv56ia	261	9	,	,	,
cord-300796-rmjv56ia	261	10	60	60	CD
cord-300796-rmjv56ia	261	11	,	,	,
cord-300796-rmjv56ia	261	12	266	266	CD
cord-300796-rmjv56ia	261	13	,	,	,
cord-300796-rmjv56ia	261	14	and	and	CC
cord-300796-rmjv56ia	261	15	328	328	LS
cord-300796-rmjv56ia	261	16	(	(	-LRB-
cord-300796-rmjv56ia	261	17	see	see	VB
cord-300796-rmjv56ia	261	18	Fig	Fig	NNP
cord-300796-rmjv56ia	261	19	.	.	NNP
cord-300796-rmjv56ia	261	20	1	1	CD
cord-300796-rmjv56ia	261	21	)	)	-RRB-
cord-300796-rmjv56ia	261	22	.	.	.
cord-300796-rmjv56ia	262	1	Fig	fig	NN
cord-300796-rmjv56ia	262	2	.	.	.
cord-300796-rmjv56ia	262	3	6	6	CD
cord-300796-rmjv56ia	263	1	(	(	-LRB-
cord-300796-rmjv56ia	263	2	lanes	lane	NNS
cord-300796-rmjv56ia	263	3	3	3	CD
cord-300796-rmjv56ia	263	4	-	-	SYM
cord-300796-rmjv56ia	263	5	16	16	CD
cord-300796-rmjv56ia	263	6	)	)	-RRB-
cord-300796-rmjv56ia	263	7	shows	show	VBZ
cord-300796-rmjv56ia	263	8	the	the	DT
cord-300796-rmjv56ia	263	9	time	time	NN
cord-300796-rmjv56ia	263	10	course	course	NN
cord-300796-rmjv56ia	263	11	of	of	IN
cord-300796-rmjv56ia	263	12	the	the	DT
cord-300796-rmjv56ia	263	13	four	four	CD
cord-300796-rmjv56ia	263	14	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	263	15	events	event	NNS
cord-300796-rmjv56ia	263	16	during	during	IN
cord-300796-rmjv56ia	263	17	C	C	NNP
cord-300796-rmjv56ia	263	18	-	-	HYPH
cord-300796-rmjv56ia	263	19	p62	p62	NN
cord-300796-rmjv56ia	263	20	d-4	d-4	NN
cord-300796-rmjv56ia	263	21	translation	translation	NN
cord-300796-rmjv56ia	263	22	.	.	.
cord-300796-rmjv56ia	264	1	A	a	DT
cord-300796-rmjv56ia	264	2	slightly	slightly	RB
cord-300796-rmjv56ia	264	3	different	different	JJ
cord-300796-rmjv56ia	264	4	protocol	protocol	NN
cord-300796-rmjv56ia	264	5	was	be	VBD
cord-300796-rmjv56ia	264	6	followed	follow	VBN
cord-300796-rmjv56ia	264	7	in	in	IN
cord-300796-rmjv56ia	264	8	this	this	DT
cord-300796-rmjv56ia	264	9	experiment	experiment	NN
cord-300796-rmjv56ia	264	10	as	as	IN
cord-300796-rmjv56ia	264	11	compared	compare	VBN
cord-300796-rmjv56ia	264	12	to	to	IN
cord-300796-rmjv56ia	264	13	that	that	DT
cord-300796-rmjv56ia	264	14	with	with	IN
cord-300796-rmjv56ia	264	15	p62-dhfr	p62-dhfr	CD
cord-300796-rmjv56ia	264	16	.	.	.
cord-300796-rmjv56ia	265	1	Seven	seven	CD
cord-300796-rmjv56ia	265	2	translations	translation	NNS
cord-300796-rmjv56ia	265	3	were	be	VBD
cord-300796-rmjv56ia	265	4	initiated	initiate	VBN
cord-300796-rmjv56ia	265	5	in	in	IN
cord-300796-rmjv56ia	265	6	parallel	parallel	NN
cord-300796-rmjv56ia	265	7	and	and	CC
cord-300796-rmjv56ia	265	8	after	after	IN
cord-300796-rmjv56ia	265	9	a	a	DT
cord-300796-rmjv56ia	265	10	3-min	3-min	JJ
cord-300796-rmjv56ia	265	11	incubation	incubation	NN
cord-300796-rmjv56ia	265	12	these	these	DT
cord-300796-rmjv56ia	265	13	were	be	VBD
cord-300796-rmjv56ia	265	14	put	put	VBN
cord-300796-rmjv56ia	265	15	on	on	IN
cord-300796-rmjv56ia	265	16	ice	ice	NN
cord-300796-rmjv56ia	265	17	and	and	CC
cord-300796-rmjv56ia	265	18	ATA	ATA	NNP
cord-300796-rmjv56ia	265	19	was	be	VBD
cord-300796-rmjv56ia	265	20	added	add	VBN
cord-300796-rmjv56ia	265	21	.	.	.
cord-300796-rmjv56ia	266	1	Elongation	elongation	NN
cord-300796-rmjv56ia	266	2	of	of	IN
cord-300796-rmjv56ia	266	3	the	the	DT
cord-300796-rmjv56ia	266	4	already	already	RB
cord-300796-rmjv56ia	266	5	initiated	initiate	VBN
cord-300796-rmjv56ia	266	6	chains	chain	NNS
cord-300796-rmjv56ia	266	7	was	be	VBD
cord-300796-rmjv56ia	266	8	then	then	RB
cord-300796-rmjv56ia	266	9	continued	continue	VBN
cord-300796-rmjv56ia	266	10	for	for	IN
cord-300796-rmjv56ia	266	11	a	a	DT
cord-300796-rmjv56ia	266	12	total	total	NN
cord-300796-rmjv56ia	266	13	of	of	IN
cord-300796-rmjv56ia	266	14	35	35	CD
cord-300796-rmjv56ia	266	15	min	min	NN
cord-300796-rmjv56ia	266	16	,	,	,
cord-300796-rmjv56ia	266	17	however	however	RB
cord-300796-rmjv56ia	266	18	,	,	,
cord-300796-rmjv56ia	266	19	so	so	IN
cord-300796-rmjv56ia	266	20	that	that	IN
cord-300796-rmjv56ia	266	21	Triton	Triton	NNP
cord-300796-rmjv56ia	266	22	X-100	x-100	NN
cord-300796-rmjv56ia	266	23	was	be	VBD
cord-300796-rmjv56ia	266	24	added	add	VBN
cord-300796-rmjv56ia	266	25	to	to	IN
cord-300796-rmjv56ia	266	26	individual	individual	JJ
cord-300796-rmjv56ia	266	27	samples	sample	NNS
cord-300796-rmjv56ia	266	28	at	at	IN
cord-300796-rmjv56ia	266	29	5	5	CD
cord-300796-rmjv56ia	266	30	,	,	,
cord-300796-rmjv56ia	266	31	10	10	CD
cord-300796-rmjv56ia	266	32	,	,	,
cord-300796-rmjv56ia	266	33	15	15	CD
cord-300796-rmjv56ia	266	34	,	,	,
cord-300796-rmjv56ia	266	35	20	20	CD
cord-300796-rmjv56ia	266	36	,	,	,
cord-300796-rmjv56ia	266	37	25	25	CD
cord-300796-rmjv56ia	266	38	,	,	,
cord-300796-rmjv56ia	266	39	30	30	CD
cord-300796-rmjv56ia	266	40	,	,	,
cord-300796-rmjv56ia	266	41	and	and	CC
cord-300796-rmjv56ia	266	42	35	35	CD
cord-300796-rmjv56ia	266	43	min	min	NN
cord-300796-rmjv56ia	266	44	.	.	.
cord-300796-rmjv56ia	267	1	At	at	IN
cord-300796-rmjv56ia	267	2	these	these	DT
cord-300796-rmjv56ia	267	3	time	time	NN
cord-300796-rmjv56ia	267	4	points	point	NNS
cord-300796-rmjv56ia	267	5	half	half	NN
cord-300796-rmjv56ia	267	6	of	of	IN
cord-300796-rmjv56ia	267	7	each	each	DT
cord-300796-rmjv56ia	267	8	sample	sample	NN
cord-300796-rmjv56ia	267	9	was	be	VBD
cord-300796-rmjv56ia	267	10	also	also	RB
cord-300796-rmjv56ia	267	11	removed	remove	VBN
cord-300796-rmjv56ia	267	12	and	and	CC
cord-300796-rmjv56ia	267	13	translation	translation	NN
cord-300796-rmjv56ia	267	14	stopped	stop	VBD
cord-300796-rmjv56ia	267	15	by	by	IN
cord-300796-rmjv56ia	267	16	cooling	cool	VBG
cord-300796-rmjv56ia	267	17	on	on	IN
cord-300796-rmjv56ia	267	18	ice	ice	NN
cord-300796-rmjv56ia	267	19	.	.	.
cord-300796-rmjv56ia	268	1	Lanes	lane	NNS
cord-300796-rmjv56ia	268	2	3	3	CD
cord-300796-rmjv56ia	268	3	-	-	SYM
cord-300796-rmjv56ia	268	4	9	9	CD
cord-300796-rmjv56ia	268	5	show	show	VBP
cord-300796-rmjv56ia	268	6	the	the	DT
cord-300796-rmjv56ia	268	7	SDS	SDS	NNP
cord-300796-rmjv56ia	268	8	-	-	HYPH
cord-300796-rmjv56ia	268	9	PAGE	PAGE	NNP
cord-300796-rmjv56ia	268	10	of	of	IN
cord-300796-rmjv56ia	268	11	the	the	DT
cord-300796-rmjv56ia	268	12	samples	sample	NNS
cord-300796-rmjv56ia	268	13	that	that	WDT
cord-300796-rmjv56ia	268	14	had	have	VBD
cord-300796-rmjv56ia	268	15	received	receive	VBN
cord-300796-rmjv56ia	268	16	Triton	Triton	NNP
cord-300796-rmjv56ia	268	17	X-100	x-100	RB
cord-300796-rmjv56ia	268	18	at	at	IN
cord-300796-rmjv56ia	268	19	different	different	JJ
cord-300796-rmjv56ia	268	20	time	time	NN
cord-300796-rmjv56ia	268	21	points	point	NNS
cord-300796-rmjv56ia	268	22	.	.	.
cord-300796-rmjv56ia	269	1	We	-PRON-	PRP
cord-300796-rmjv56ia	269	2	found	find	VBD
cord-300796-rmjv56ia	269	3	the	the	DT
cord-300796-rmjv56ia	269	4	sequential	sequential	JJ
cord-300796-rmjv56ia	269	5	appearance	appearance	NN
cord-300796-rmjv56ia	269	6	of	of	IN
cord-300796-rmjv56ia	269	7	p62	p62	NN
cord-300796-rmjv56ia	269	8	d-4	d-4	NN
cord-300796-rmjv56ia	269	9	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	269	10	with	with	IN
cord-300796-rmjv56ia	269	11	no	no	DT
cord-300796-rmjv56ia	269	12	carbohydrate	carbohydrate	NN
cord-300796-rmjv56ia	269	13	(	(	-LRB-
cord-300796-rmjv56ia	269	14	lane	lane	NN
cord-300796-rmjv56ia	269	15	3	3	CD
cord-300796-rmjv56ia	269	16	)	)	-RRB-
cord-300796-rmjv56ia	269	17	,	,	,
cord-300796-rmjv56ia	269	18	with	with	IN
cord-300796-rmjv56ia	269	19	one	one	CD
cord-300796-rmjv56ia	269	20	and	and	CC
cord-300796-rmjv56ia	269	21	two	two	CD
cord-300796-rmjv56ia	269	22	units	unit	NNS
cord-300796-rmjv56ia	269	23	added	add	VBN
cord-300796-rmjv56ia	269	24	(	(	-LRB-
cord-300796-rmjv56ia	269	25	seen	see	VBN
cord-300796-rmjv56ia	269	26	as	as	IN
cord-300796-rmjv56ia	269	27	two	two	CD
cord-300796-rmjv56ia	269	28	new	new	JJ
cord-300796-rmjv56ia	269	29	bands	band	NNS
cord-300796-rmjv56ia	269	30	with	with	IN
cord-300796-rmjv56ia	269	31	slower	slow	JJR
cord-300796-rmjv56ia	269	32	migration	migration	NN
cord-300796-rmjv56ia	269	33	in	in	IN
cord-300796-rmjv56ia	269	34	lanes	lane	NNS
cord-300796-rmjv56ia	269	35	4	4	CD
cord-300796-rmjv56ia	269	36	and	and	CC
cord-300796-rmjv56ia	269	37	5	5	CD
cord-300796-rmjv56ia	269	38	)	)	-RRB-
cord-300796-rmjv56ia	269	39	,	,	,
cord-300796-rmjv56ia	269	40	with	with	IN
cord-300796-rmjv56ia	269	41	three	three	CD
cord-300796-rmjv56ia	269	42	units	unit	NNS
cord-300796-rmjv56ia	269	43	(	(	-LRB-
cord-300796-rmjv56ia	269	44	lane	lane	NNP
cord-300796-rmjv56ia	269	45	6	6	CD
cord-300796-rmjv56ia	269	46	)	)	-RRB-
cord-300796-rmjv56ia	269	47	,	,	,
cord-300796-rmjv56ia	269	48	and	and	CC
cord-300796-rmjv56ia	269	49	all	all	DT
cord-300796-rmjv56ia	269	50	four	four	CD
cord-300796-rmjv56ia	269	51	sugar	sugar	NN
cord-300796-rmjv56ia	269	52	units	unit	NNS
cord-300796-rmjv56ia	269	53	(	(	-LRB-
cord-300796-rmjv56ia	269	54	lanes	lane	NNS
cord-300796-rmjv56ia	269	55	7	7	CD
cord-300796-rmjv56ia	269	56	,	,	,
cord-300796-rmjv56ia	269	57	8	8	CD
cord-300796-rmjv56ia	269	58	,	,	,
cord-300796-rmjv56ia	269	59	and	and	CC
cord-300796-rmjv56ia	269	60	9	9	LS
cord-300796-rmjv56ia	269	61	)	)	-RRB-
cord-300796-rmjv56ia	269	62	attached	attach	VBN
cord-300796-rmjv56ia	269	63	to	to	IN
cord-300796-rmjv56ia	269	64	the	the	DT
cord-300796-rmjv56ia	269	65	protein	protein	NN
cord-300796-rmjv56ia	269	66	backbone	backbone	NN
cord-300796-rmjv56ia	269	67	as	as	IN
cord-300796-rmjv56ia	269	68	the	the	DT
cord-300796-rmjv56ia	269	69	translation	translation	NN
cord-300796-rmjv56ia	269	70	proceeded	proceed	VBD
cord-300796-rmjv56ia	269	71	coordinately	coordinately	RB
cord-300796-rmjv56ia	269	72	with	with	IN
cord-300796-rmjv56ia	269	73	time	time	NN
cord-300796-rmjv56ia	269	74	.	.	.
cord-300796-rmjv56ia	270	1	Note	note	VB
cord-300796-rmjv56ia	270	2	that	that	IN
cord-300796-rmjv56ia	270	3	the	the	DT
cord-300796-rmjv56ia	270	4	four	four	CD
cord-300796-rmjv56ia	270	5	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	270	6	events	event	NNS
cord-300796-rmjv56ia	270	7	result	result	VBP
cord-300796-rmjv56ia	270	8	in	in	IN
cord-300796-rmjv56ia	270	9	different	different	JJ
cord-300796-rmjv56ia	270	10	degrees	degree	NNS
cord-300796-rmjv56ia	270	11	of	of	IN
cord-300796-rmjv56ia	270	12	increase	increase	NN
cord-300796-rmjv56ia	270	13	of	of	IN
cord-300796-rmjv56ia	270	14	the	the	DT
cord-300796-rmjv56ia	270	15	size	size	NN
cord-300796-rmjv56ia	270	16	of	of	IN
cord-300796-rmjv56ia	270	17	p62	p62	NN
cord-300796-rmjv56ia	270	18	d-4	d-4	NN
cord-300796-rmjv56ia	270	19	.	.	.
cord-300796-rmjv56ia	271	1	The	the	DT
cord-300796-rmjv56ia	271	2	second	second	JJ
cord-300796-rmjv56ia	271	3	event	event	NN
cord-300796-rmjv56ia	271	4	causes	cause	VBZ
cord-300796-rmjv56ia	271	5	the	the	DT
cord-300796-rmjv56ia	271	6	largest	large	JJS
cord-300796-rmjv56ia	271	7	increase	increase	NN
cord-300796-rmjv56ia	271	8	and	and	CC
cord-300796-rmjv56ia	271	9	the	the	DT
cord-300796-rmjv56ia	271	10	third	third	JJ
cord-300796-rmjv56ia	271	11	one	one	NN
cord-300796-rmjv56ia	271	12	the	the	DT
cord-300796-rmjv56ia	271	13	smallest	small	JJS
cord-300796-rmjv56ia	271	14	.	.	.
cord-300796-rmjv56ia	272	1	As	as	IN
cord-300796-rmjv56ia	272	2	the	the	DT
cord-300796-rmjv56ia	272	3	sugar	sugar	NN
cord-300796-rmjv56ia	272	4	unit	unit	NN
cord-300796-rmjv56ia	272	5	added	add	VBD
cord-300796-rmjv56ia	272	6	at	at	IN
cord-300796-rmjv56ia	272	7	each	each	DT
cord-300796-rmjv56ia	272	8	step	step	NN
cord-300796-rmjv56ia	272	9	should	should	MD
cord-300796-rmjv56ia	272	10	be	be	VB
cord-300796-rmjv56ia	272	11	the	the	DT
cord-300796-rmjv56ia	272	12	same	same	JJ
cord-300796-rmjv56ia	272	13	we	-PRON-	PRP
cord-300796-rmjv56ia	272	14	think	think	VBP
cord-300796-rmjv56ia	272	15	that	that	IN
cord-300796-rmjv56ia	272	16	these	these	DT
cord-300796-rmjv56ia	272	17	differences	difference	NNS
cord-300796-rmjv56ia	272	18	reflect	reflect	VBP
cord-300796-rmjv56ia	272	19	some	some	DT
cord-300796-rmjv56ia	272	20	conformational	conformational	JJ
cord-300796-rmjv56ia	272	21	changes	change	NNS
cord-300796-rmjv56ia	272	22	in	in	IN
cord-300796-rmjv56ia	272	23	the	the	DT
cord-300796-rmjv56ia	272	24	p62	p62	NN
cord-300796-rmjv56ia	272	25	folding	folding	NN
cord-300796-rmjv56ia	272	26	which	which	WDT
cord-300796-rmjv56ia	272	27	occur	occur	VBP
cord-300796-rmjv56ia	272	28	coordinately	coordinately	RB
cord-300796-rmjv56ia	272	29	with	with	IN
cord-300796-rmjv56ia	272	30	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	272	31	.	.	.
cord-300796-rmjv56ia	273	1	In	in	IN
cord-300796-rmjv56ia	273	2	lanes	lane	NNS
cord-300796-rmjv56ia	273	3	10	10	CD
cord-300796-rmjv56ia	273	4	-	-	:
cord-300796-rmjv56ia	273	5	-16	-16	NN
cord-300796-rmjv56ia	273	6	we	-PRON-	PRP
cord-300796-rmjv56ia	273	7	have	have	VBP
cord-300796-rmjv56ia	273	8	analyzed	analyze	VBN
cord-300796-rmjv56ia	273	9	the	the	DT
cord-300796-rmjv56ia	273	10	samples	sample	NNS
cord-300796-rmjv56ia	273	11	that	that	WDT
cord-300796-rmjv56ia	273	12	were	be	VBD
cord-300796-rmjv56ia	273	13	withdrawn	withdraw	VBN
cord-300796-rmjv56ia	273	14	at	at	IN
cord-300796-rmjv56ia	273	15	the	the	DT
cord-300796-rmjv56ia	273	16	different	different	JJ
cord-300796-rmjv56ia	273	17	times	time	NNS
cord-300796-rmjv56ia	273	18	but	but	CC
cord-300796-rmjv56ia	273	19	were	be	VBD
cord-300796-rmjv56ia	273	20	kept	keep	VBN
cord-300796-rmjv56ia	273	21	on	on	IN
cord-300796-rmjv56ia	273	22	ice	ice	NN
cord-300796-rmjv56ia	273	23	.	.	.
cord-300796-rmjv56ia	274	1	As	as	IN
cord-300796-rmjv56ia	274	2	expected	expect	VBN
cord-300796-rmjv56ia	274	3	,	,	,
cord-300796-rmjv56ia	274	4	we	-PRON-	PRP
cord-300796-rmjv56ia	274	5	see	see	VBP
cord-300796-rmjv56ia	274	6	a	a	DT
cord-300796-rmjv56ia	274	7	sequential	sequential	JJ
cord-300796-rmjv56ia	274	8	appearance	appearance	NN
cord-300796-rmjv56ia	274	9	of	of	IN
cord-300796-rmjv56ia	274	10	first	first	RB
cord-300796-rmjv56ia	274	11	the	the	DT
cord-300796-rmjv56ia	274	12	capsid	capsid	NN
cord-300796-rmjv56ia	274	13	The	the	DT
cord-300796-rmjv56ia	274	14	Journal	Journal	NNP
cord-300796-rmjv56ia	274	15	of	of	IN
cord-300796-rmjv56ia	274	16	Cell	Cell	NNP
cord-300796-rmjv56ia	274	17	Biology	Biology	NNP
cord-300796-rmjv56ia	274	18	,	,	,
cord-300796-rmjv56ia	274	19	Volume	volume	NN
cord-300796-rmjv56ia	274	20	111	111	CD
cord-300796-rmjv56ia	274	21	,	,	,
cord-300796-rmjv56ia	274	22	1990	1990	CD
cord-300796-rmjv56ia	274	23	protein	protein	NN
cord-300796-rmjv56ia	274	24	(	(	-LRB-
cord-300796-rmjv56ia	274	25	in	in	IN
cord-300796-rmjv56ia	274	26	the	the	DT
cord-300796-rmjv56ia	274	27	10-min	10-min	CD
cord-300796-rmjv56ia	274	28	sample	sample	NN
cord-300796-rmjv56ia	274	29	)	)	-RRB-
cord-300796-rmjv56ia	274	30	and	and	CC
cord-300796-rmjv56ia	274	31	then	then	RB
cord-300796-rmjv56ia	274	32	the	the	DT
cord-300796-rmjv56ia	274	33	p62	p62	NN
cord-300796-rmjv56ia	274	34	d-4	d-4	NN
cord-300796-rmjv56ia	274	35	protein	protein	NN
cord-300796-rmjv56ia	274	36	(	(	-LRB-
cord-300796-rmjv56ia	274	37	barely	barely	RB
cord-300796-rmjv56ia	274	38	visible	visible	JJ
cord-300796-rmjv56ia	274	39	in	in	IN
cord-300796-rmjv56ia	274	40	the	the	DT
cord-300796-rmjv56ia	274	41	20-min	20-min	CD
cord-300796-rmjv56ia	274	42	sample	sample	NN
cord-300796-rmjv56ia	274	43	)	)	-RRB-
cord-300796-rmjv56ia	274	44	.	.	.
cord-300796-rmjv56ia	275	1	The	the	DT
cord-300796-rmjv56ia	275	2	p62	p62	NN
cord-300796-rmjv56ia	275	3	d-4	d-4	NN
cord-300796-rmjv56ia	275	4	protein	protein	NN
cord-300796-rmjv56ia	275	5	is	be	VBZ
cord-300796-rmjv56ia	275	6	partly	partly	RB
cord-300796-rmjv56ia	275	7	present	present	JJ
cord-300796-rmjv56ia	275	8	in	in	IN
cord-300796-rmjv56ia	275	9	its	-PRON-	PRP$
cord-300796-rmjv56ia	275	10	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	275	11	and	and	CC
cord-300796-rmjv56ia	275	12	partly	partly	RB
cord-300796-rmjv56ia	275	13	in	in	IN
cord-300796-rmjv56ia	275	14	its	-PRON-	PRP$
cord-300796-rmjv56ia	275	15	unglycosylated	unglycosylated	JJ
cord-300796-rmjv56ia	275	16	form	form	NN
cord-300796-rmjv56ia	275	17	.	.	.
cord-300796-rmjv56ia	276	1	Using	use	VBG
cord-300796-rmjv56ia	276	2	21.5	21.5	CD
cord-300796-rmjv56ia	276	3	min	min	NN
cord-300796-rmjv56ia	276	4	as	as	IN
cord-300796-rmjv56ia	276	5	a	a	DT
cord-300796-rmjv56ia	276	6	rough	rough	JJ
cord-300796-rmjv56ia	276	7	estimate	estimate	NN
cord-300796-rmjv56ia	276	8	for	for	IN
cord-300796-rmjv56ia	276	9	the	the	DT
cord-300796-rmjv56ia	276	10	translation	translation	NN
cord-300796-rmjv56ia	276	11	time	time	NN
cord-300796-rmjv56ia	276	12	of	of	IN
cord-300796-rmjv56ia	276	13	the	the	DT
cord-300796-rmjv56ia	276	14	746	746	CD
cord-300796-rmjv56ia	276	15	residue	residue	NN
cord-300796-rmjv56ia	276	16	long	long	JJ
cord-300796-rmjv56ia	276	17	C	c	NN
cord-300796-rmjv56ia	276	18	-	-	HYPH
cord-300796-rmjv56ia	276	19	p62	p62	NN
cord-300796-rmjv56ia	276	20	d-4	d-4	NN
cord-300796-rmjv56ia	276	21	chain	chain	NN
cord-300796-rmjv56ia	276	22	(	(	-LRB-
cord-300796-rmjv56ia	276	23	time	time	NN
cord-300796-rmjv56ia	276	24	point	point	NN
cord-300796-rmjv56ia	276	25	of	of	IN
cord-300796-rmjv56ia	276	26	p62	p62	NN
cord-300796-rmjv56ia	276	27	d-4	d-4	NN
cord-300796-rmjv56ia	276	28	detection	detection	NN
cord-300796-rmjv56ia	276	29	,	,	,
cord-300796-rmjv56ia	276	30	20	20	CD
cord-300796-rmjv56ia	276	31	min	min	NN
cord-300796-rmjv56ia	276	32	,	,	,
cord-300796-rmjv56ia	276	33	plus	plus	CC
cord-300796-rmjv56ia	276	34	half	half	NN
cord-300796-rmjv56ia	276	35	of	of	IN
cord-300796-rmjv56ia	276	36	the	the	DT
cord-300796-rmjv56ia	276	37	3-min	3-min	JJ
cord-300796-rmjv56ia	276	38	preincubation	preincubation	NN
cord-300796-rmjv56ia	276	39	time	time	NN
cord-300796-rmjv56ia	276	40	without	without	IN
cord-300796-rmjv56ia	276	41	ATA	ATA	NNP
cord-300796-rmjv56ia	276	42	)	)	-RRB-
cord-300796-rmjv56ia	276	43	we	-PRON-	PRP
cord-300796-rmjv56ia	276	44	have	have	VBP
cord-300796-rmjv56ia	276	45	calculated	calculate	VBN
cord-300796-rmjv56ia	276	46	the	the	DT
cord-300796-rmjv56ia	276	47	translation	translation	NN
cord-300796-rmjv56ia	276	48	rate	rate	NN
cord-300796-rmjv56ia	276	49	and	and	CC
cord-300796-rmjv56ia	276	50	derived	derive	VBD
cord-300796-rmjv56ia	276	51	the	the	DT
cord-300796-rmjv56ia	276	52	approximate	approximate	JJ
cord-300796-rmjv56ia	276	53	earliest	early	JJS
cord-300796-rmjv56ia	276	54	time	time	NN
cord-300796-rmjv56ia	276	55	points	point	NNS
cord-300796-rmjv56ia	276	56	when	when	WRB
cord-300796-rmjv56ia	276	57	the	the	DT
cord-300796-rmjv56ia	276	58	four	four	CD
cord-300796-rmjv56ia	276	59	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	276	60	sites	site	NNS
cord-300796-rmjv56ia	276	61	of	of	IN
cord-300796-rmjv56ia	276	62	p62	p62	NN
cord-300796-rmjv56ia	276	63	d-4	d-4	NN
cord-300796-rmjv56ia	276	64	should	should	MD
cord-300796-rmjv56ia	276	65	be	be	VB
cord-300796-rmjv56ia	276	66	available	available	JJ
cord-300796-rmjv56ia	276	67	for	for	IN
cord-300796-rmjv56ia	276	68	modification	modification	NN
cord-300796-rmjv56ia	276	69	.	.	.
cord-300796-rmjv56ia	277	1	According	accord	VBG
cord-300796-rmjv56ia	277	2	to	to	IN
cord-300796-rmjv56ia	277	3	these	these	DT
cord-300796-rmjv56ia	277	4	,	,	,
cord-300796-rmjv56ia	277	5	Asn~	asn~	NN
cord-300796-rmjv56ia	277	6	and	and	CC
cord-300796-rmjv56ia	277	7	Asn60	Asn60	NNP
cord-300796-rmjv56ia	277	8	should	should	MD
cord-300796-rmjv56ia	277	9	be	be	VB
cord-300796-rmjv56ia	277	10	the	the	DT
cord-300796-rmjv56ia	277	11	only	only	JJ
cord-300796-rmjv56ia	277	12	sites	site	NNS
cord-300796-rmjv56ia	277	13	available	available	JJ
cord-300796-rmjv56ia	277	14	for	for	IN
cord-300796-rmjv56ia	277	15	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	277	16	in	in	IN
cord-300796-rmjv56ia	277	17	the	the	DT
cord-300796-rmjv56ia	277	18	10-min	10-min	CD
cord-300796-rmjv56ia	277	19	sample	sample	NN
cord-300796-rmjv56ia	277	20	,	,	,
cord-300796-rmjv56ia	277	21	shown	show	VBN
cord-300796-rmjv56ia	277	22	in	in	IN
cord-300796-rmjv56ia	277	23	lane	lane	NN
cord-300796-rmjv56ia	277	24	4	4	CD
cord-300796-rmjv56ia	277	25	,	,	,
cord-300796-rmjv56ia	277	26	and	and	CC
cord-300796-rmjv56ia	277	27	the	the	DT
cord-300796-rmjv56ia	277	28	most	most	RBS
cord-300796-rmjv56ia	277	29	abundant	abundant	JJ
cord-300796-rmjv56ia	277	30	ones	one	NNS
cord-300796-rmjv56ia	277	31	presented	present	VBN
cord-300796-rmjv56ia	277	32	for	for	IN
cord-300796-rmjv56ia	277	33	modification	modification	NN
cord-300796-rmjv56ia	277	34	in	in	IN
cord-300796-rmjv56ia	277	35	the	the	DT
cord-300796-rmjv56ia	277	36	15-min	15-min	CD
cord-300796-rmjv56ia	277	37	sample	sample	NN
cord-300796-rmjv56ia	277	38	,	,	,
cord-300796-rmjv56ia	277	39	shown	show	VBN
cord-300796-rmjv56ia	277	40	in	in	IN
cord-300796-rmjv56ia	277	41	lane	lane	NN
cord-300796-rmjv56ia	277	42	5	5	CD
cord-300796-rmjv56ia	277	43	.	.	.
cord-300796-rmjv56ia	278	1	Therefore	therefore	RB
cord-300796-rmjv56ia	278	2	,	,	,
cord-300796-rmjv56ia	278	3	it	-PRON-	PRP
cord-300796-rmjv56ia	278	4	appears	appear	VBZ
cord-300796-rmjv56ia	278	5	reasonable	reasonable	JJ
cord-300796-rmjv56ia	278	6	to	to	TO
cord-300796-rmjv56ia	278	7	assume	assume	VB
cord-300796-rmjv56ia	278	8	that	that	IN
cord-300796-rmjv56ia	278	9	those	those	DT
cord-300796-rmjv56ia	278	10	chains	chain	NNS
cord-300796-rmjv56ia	278	11	of	of	IN
cord-300796-rmjv56ia	278	12	these	these	DT
cord-300796-rmjv56ia	278	13	two	two	CD
cord-300796-rmjv56ia	278	14	samples	sample	NNS
cord-300796-rmjv56ia	278	15	which	which	WDT
cord-300796-rmjv56ia	278	16	have	have	VBP
cord-300796-rmjv56ia	278	17	obtained	obtain	VBN
cord-300796-rmjv56ia	278	18	two	two	CD
cord-300796-rmjv56ia	278	19	sugar	sugar	NN
cord-300796-rmjv56ia	278	20	units	unit	NNS
cord-300796-rmjv56ia	278	21	carry	carry	VBP
cord-300796-rmjv56ia	278	22	these	these	DT
cord-300796-rmjv56ia	278	23	on	on	IN
cord-300796-rmjv56ia	278	24	the	the	DT
cord-300796-rmjv56ia	278	25	aforementioned	aforementioned	JJ
cord-300796-rmjv56ia	278	26	two	two	CD
cord-300796-rmjv56ia	278	27	sites	site	NNS
cord-300796-rmjv56ia	278	28	.	.	.
cord-300796-rmjv56ia	279	1	Thus	thus	RB
cord-300796-rmjv56ia	279	2	,	,	,
cord-300796-rmjv56ia	279	3	the	the	DT
cord-300796-rmjv56ia	279	4	peptide	peptide	NN
cord-300796-rmjv56ia	279	5	region	region	NN
cord-300796-rmjv56ia	279	6	with	with	IN
cord-300796-rmjv56ia	279	7	Asn	Asn	NNP
cord-300796-rmjv56ia	279	8	,	,	,
cord-300796-rmjv56ia	279	9	a	a	DT
cord-300796-rmjv56ia	279	10	seems	seem	VBZ
cord-300796-rmjv56ia	279	11	to	to	TO
cord-300796-rmjv56ia	279	12	be	be	VB
cord-300796-rmjv56ia	279	13	target	target	NN
cord-300796-rmjv56ia	279	14	for	for	IN
cord-300796-rmjv56ia	279	15	rapid	rapid	JJ
cord-300796-rmjv56ia	279	16	modification	modification	NN
cord-300796-rmjv56ia	279	17	also	also	RB
cord-300796-rmjv56ia	279	18	when	when	WRB
cord-300796-rmjv56ia	279	19	present	present	JJ
cord-300796-rmjv56ia	279	20	in	in	IN
cord-300796-rmjv56ia	279	21	its	-PRON-	PRP$
cord-300796-rmjv56ia	279	22	normal	normal	JJ
cord-300796-rmjv56ia	279	23	background	background	NN
cord-300796-rmjv56ia	279	24	,	,	,
cord-300796-rmjv56ia	279	25	that	that	RB
cord-300796-rmjv56ia	279	26	is	be	VBZ
cord-300796-rmjv56ia	279	27	with	with	IN
cord-300796-rmjv56ia	279	28	the	the	DT
cord-300796-rmjv56ia	279	29	p62	p62	NN
cord-300796-rmjv56ia	279	30	protein	protein	NN
cord-300796-rmjv56ia	279	31	.	.	.
cord-300796-rmjv56ia	280	1	The	the	DT
cord-300796-rmjv56ia	280	2	fact	fact	NN
cord-300796-rmjv56ia	280	3	that	that	IN
cord-300796-rmjv56ia	280	4	the	the	DT
cord-300796-rmjv56ia	280	5	40	40	CD
cord-300796-rmjv56ia	280	6	residue	residue	NN
cord-300796-rmjv56ia	280	7	fragment	fragment	NN
cord-300796-rmjv56ia	280	8	of	of	IN
cord-300796-rmjv56ia	280	9	the	the	DT
cord-300796-rmjv56ia	280	10	NH2-terminal	nh2-terminal	JJ
cord-300796-rmjv56ia	280	11	region	region	NN
cord-300796-rmjv56ia	280	12	of	of	IN
cord-300796-rmjv56ia	280	13	the	the	DT
cord-300796-rmjv56ia	280	14	p62	p62	NN
cord-300796-rmjv56ia	280	15	protein	protein	NN
cord-300796-rmjv56ia	280	16	is	be	VBZ
cord-300796-rmjv56ia	280	17	able	able	JJ
cord-300796-rmjv56ia	280	18	to	to	TO
cord-300796-rmjv56ia	280	19	translocate	translocate	VB
cord-300796-rmjv56ia	280	20	two	two	CD
cord-300796-rmjv56ia	280	21	different	different	JJ
cord-300796-rmjv56ia	280	22	reporter	reporter	NN
cord-300796-rmjv56ia	280	23	molecules	molecule	NNS
cord-300796-rmjv56ia	280	24	into	into	IN
cord-300796-rmjv56ia	280	25	microsomes	microsome	NNS
cord-300796-rmjv56ia	280	26	constitutes	constitute	VBZ
cord-300796-rmjv56ia	280	27	in	in	IN
cord-300796-rmjv56ia	280	28	our	-PRON-	PRP$
cord-300796-rmjv56ia	280	29	mind	mind	NN
cord-300796-rmjv56ia	280	30	convincing	convincing	JJ
cord-300796-rmjv56ia	280	31	evidence	evidence	NN
cord-300796-rmjv56ia	280	32	for	for	IN
cord-300796-rmjv56ia	280	33	signal	signal	NN
cord-300796-rmjv56ia	280	34	sequence	sequence	NN
cord-300796-rmjv56ia	280	35	activity	activity	NN
cord-300796-rmjv56ia	280	36	in	in	IN
cord-300796-rmjv56ia	280	37	this	this	DT
cord-300796-rmjv56ia	280	38	protein	protein	NN
cord-300796-rmjv56ia	280	39	fragment	fragment	NN
cord-300796-rmjv56ia	280	40	.	.	.
cord-300796-rmjv56ia	281	1	A	a	DT
cord-300796-rmjv56ia	281	2	more	more	RBR
cord-300796-rmjv56ia	281	3	precise	precise	JJ
cord-300796-rmjv56ia	281	4	location	location	NN
cord-300796-rmjv56ia	281	5	of	of	IN
cord-300796-rmjv56ia	281	6	the	the	DT
cord-300796-rmjv56ia	281	7	p62	p62	NN
cord-300796-rmjv56ia	281	8	signal	signal	NN
cord-300796-rmjv56ia	281	9	sequence	sequence	NN
cord-300796-rmjv56ia	281	10	within	within	IN
cord-300796-rmjv56ia	281	11	the	the	DT
cord-300796-rmjv56ia	281	12	40	40	CD
cord-300796-rmjv56ia	281	13	residue	residue	NN
cord-300796-rmjv56ia	281	14	p62	p62	NN
cord-300796-rmjv56ia	281	15	fragment	fragment	NN
cord-300796-rmjv56ia	281	16	can	can	MD
cord-300796-rmjv56ia	281	17	be	be	VB
cord-300796-rmjv56ia	281	18	done	do	VBN
cord-300796-rmjv56ia	281	19	with	with	IN
cord-300796-rmjv56ia	281	20	the	the	DT
cord-300796-rmjv56ia	281	21	aid	aid	NN
cord-300796-rmjv56ia	281	22	of	of	IN
cord-300796-rmjv56ia	281	23	the	the	DT
cord-300796-rmjv56ia	281	24	known	know	VBN
cord-300796-rmjv56ia	281	25	consensus	consensus	NN
cord-300796-rmjv56ia	281	26	features	feature	NNS
cord-300796-rmjv56ia	281	27	of	of	IN
cord-300796-rmjv56ia	281	28	a	a	DT
cord-300796-rmjv56ia	281	29	signal	signal	JJ
cord-300796-rmjv56ia	281	30	sequence	sequence	NN
cord-300796-rmjv56ia	281	31	.	.	.
cord-300796-rmjv56ia	282	1	The	the	DT
cord-300796-rmjv56ia	282	2	most	most	RBS
cord-300796-rmjv56ia	282	3	typical	typical	JJ
cord-300796-rmjv56ia	282	4	characteristic	characteristic	NN
cord-300796-rmjv56ia	282	5	of	of	IN
cord-300796-rmjv56ia	282	6	a	a	DT
cord-300796-rmjv56ia	282	7	signal	signal	JJ
cord-300796-rmjv56ia	282	8	sequence	sequence	NN
cord-300796-rmjv56ia	282	9	is	be	VBZ
cord-300796-rmjv56ia	282	10	a	a	DT
cord-300796-rmjv56ia	282	11	stretch	stretch	NN
cord-300796-rmjv56ia	282	12	of	of	IN
cord-300796-rmjv56ia	282	13	10	10	CD
cord-300796-rmjv56ia	282	14	-	-	SYM
cord-300796-rmjv56ia	282	15	12	12	CD
cord-300796-rmjv56ia	282	16	uncharged	uncharged	JJ
cord-300796-rmjv56ia	282	17	residues	residue	NNS
cord-300796-rmjv56ia	282	18	,	,	,
cord-300796-rmjv56ia	282	19	mostly	mostly	RB
cord-300796-rmjv56ia	282	20	hydrophobic	hydrophobic	JJ
cord-300796-rmjv56ia	282	21	ones	one	NNS
cord-300796-rmjv56ia	282	22	(	(	-LRB-
cord-300796-rmjv56ia	282	23	von	von	NNP
cord-300796-rmjv56ia	282	24	Heijne	Heijne	NNP
cord-300796-rmjv56ia	282	25	,	,	,
cord-300796-rmjv56ia	282	26	1985	1985	CD
cord-300796-rmjv56ia	282	27	)	)	-RRB-
cord-300796-rmjv56ia	282	28	.	.	.
cord-300796-rmjv56ia	283	1	This	this	DT
cord-300796-rmjv56ia	283	2	part	part	NN
cord-300796-rmjv56ia	283	3	of	of	IN
cord-300796-rmjv56ia	283	4	the	the	DT
cord-300796-rmjv56ia	283	5	signal	signal	NN
cord-300796-rmjv56ia	283	6	sequence	sequence	NN
cord-300796-rmjv56ia	283	7	probably	probably	RB
cord-300796-rmjv56ia	283	8	forms	form	VBZ
cord-300796-rmjv56ia	283	9	an	an	DT
cord-300796-rmjv56ia	283	10	alpha	alpha	NN
cord-300796-rmjv56ia	283	11	helix	helix	NN
cord-300796-rmjv56ia	283	12	in	in	IN
cord-300796-rmjv56ia	283	13	the	the	DT
cord-300796-rmjv56ia	283	14	ER	ER	NNP
cord-300796-rmjv56ia	283	15	membrane	membrane	NN
cord-300796-rmjv56ia	283	16	(	(	-LRB-
cord-300796-rmjv56ia	283	17	Emr	Emr	NNP
cord-300796-rmjv56ia	283	18	and	and	CC
cord-300796-rmjv56ia	283	19	Silhavy	Silhavy	NNP
cord-300796-rmjv56ia	283	20	,	,	,
cord-300796-rmjv56ia	283	21	1983	1983	CD
cord-300796-rmjv56ia	283	22	;	;	:
cord-300796-rmjv56ia	284	1	Briggs	Briggs	NNP
cord-300796-rmjv56ia	284	2	et	et	NNP
cord-300796-rmjv56ia	284	3	al	al	NNP
cord-300796-rmjv56ia	284	4	.	.	.
cord-300796-rmjv56ia	285	1	,	,	,
cord-300796-rmjv56ia	285	2	1985	1985	CD
cord-300796-rmjv56ia	286	1	Briggs	Briggs	NNP
cord-300796-rmjv56ia	286	2	et	et	NNP
cord-300796-rmjv56ia	286	3	al	al	NNP
cord-300796-rmjv56ia	286	4	.	.	.
cord-300796-rmjv56ia	287	1	,	,	,
cord-300796-rmjv56ia	287	2	,	,	,
cord-300796-rmjv56ia	287	3	1986	1986	CD
cord-300796-rmjv56ia	287	4	Kendall	Kendall	NNP
cord-300796-rmjv56ia	287	5	et	et	NNP
cord-300796-rmjv56ia	287	6	al	al	NNP
cord-300796-rmjv56ia	287	7	.	.	NNP
cord-300796-rmjv56ia	287	8	,	,	,
cord-300796-rmjv56ia	287	9	1986	1986	CD
cord-300796-rmjv56ia	287	10	;	;	.
cord-300796-rmjv56ia	288	1	Batenburg	Batenburg	NNP
cord-300796-rmjv56ia	288	2	et	et	NNP
cord-300796-rmjv56ia	288	3	al	al	NNP
cord-300796-rmjv56ia	288	4	.	.	NNP
cord-300796-rmjv56ia	288	5	,	,	,
cord-300796-rmjv56ia	288	6	1988	1988	CD
cord-300796-rmjv56ia	288	7	)	)	-RRB-
cord-300796-rmjv56ia	288	8	.	.	.
cord-300796-rmjv56ia	289	1	The	the	DT
cord-300796-rmjv56ia	289	2	only	only	JJ
cord-300796-rmjv56ia	289	3	possible	possible	JJ
cord-300796-rmjv56ia	289	4	candidate	candidate	NN
cord-300796-rmjv56ia	289	5	region	region	NN
cord-300796-rmjv56ia	289	6	within	within	IN
cord-300796-rmjv56ia	289	7	the	the	DT
cord-300796-rmjv56ia	289	8	40	40	CD
cord-300796-rmjv56ia	289	9	residue	residue	NN
cord-300796-rmjv56ia	289	10	p62	p62	NN
cord-300796-rmjv56ia	289	11	fragment	fragment	NN
cord-300796-rmjv56ia	289	12	having	have	VBG
cord-300796-rmjv56ia	289	13	these	these	DT
cord-300796-rmjv56ia	289	14	features	feature	NNS
cord-300796-rmjv56ia	289	15	is	be	VBZ
cord-300796-rmjv56ia	289	16	the	the	DT
cord-300796-rmjv56ia	289	17	13	13	CD
cord-300796-rmjv56ia	289	18	residue	residue	NN
cord-300796-rmjv56ia	289	19	segment	segment	NN
cord-300796-rmjv56ia	289	20	between	between	IN
cord-300796-rmjv56ia	289	21	Pro	Pro	NNP
cord-300796-rmjv56ia	289	22	3	3	CD
cord-300796-rmjv56ia	289	23	and	and	CC
cord-300796-rmjv56ia	289	24	Pro	Pro	NNP
cord-300796-rmjv56ia	289	25	17	17	CD
cord-300796-rmjv56ia	289	26	(	(	-LRB-
cord-300796-rmjv56ia	289	27	see	see	VB
cord-300796-rmjv56ia	289	28	box	box	NN
cord-300796-rmjv56ia	289	29	in	in	IN
cord-300796-rmjv56ia	289	30	uppermost	uppermost	JJ
cord-300796-rmjv56ia	289	31	sequence	sequence	NN
cord-300796-rmjv56ia	289	32	in	in	IN
cord-300796-rmjv56ia	289	33	Fig	Fig	NNP
cord-300796-rmjv56ia	289	34	.	.	NNP
cord-300796-rmjv56ia	289	35	7	7	CD
cord-300796-rmjv56ia	289	36	)	)	-RRB-
cord-300796-rmjv56ia	289	37	.	.	.
cord-300796-rmjv56ia	290	1	The	the	DT
cord-300796-rmjv56ia	290	2	Pro	pro	JJ
cord-300796-rmjv56ia	290	3	-	-	JJ
cord-300796-rmjv56ia	290	4	rich	rich	JJ
cord-300796-rmjv56ia	290	5	region	region	NN
cord-300796-rmjv56ia	290	6	in	in	IN
cord-300796-rmjv56ia	290	7	the	the	DT
cord-300796-rmjv56ia	290	8	middle	middle	NN
cord-300796-rmjv56ia	290	9	of	of	IN
cord-300796-rmjv56ia	290	10	the	the	DT
cord-300796-rmjv56ia	290	11	40	40	CD
cord-300796-rmjv56ia	290	12	residue	residue	NN
cord-300796-rmjv56ia	290	13	fragment	fragment	NN
cord-300796-rmjv56ia	290	14	would	would	MD
cord-300796-rmjv56ia	290	15	not	not	RB
cord-300796-rmjv56ia	290	16	form	form	VB
cord-300796-rmjv56ia	290	17	an	an	DT
cord-300796-rmjv56ia	290	18	alpha	alpha	NN
cord-300796-rmjv56ia	290	19	-	-	HYPH
cord-300796-rmjv56ia	290	20	helix	helix	NN
cord-300796-rmjv56ia	290	21	,	,	,
cord-300796-rmjv56ia	290	22	and	and	CC
cord-300796-rmjv56ia	290	23	the	the	DT
cord-300796-rmjv56ia	290	24	COOH	COOH	NNP
cord-300796-rmjv56ia	290	25	-	-	HYPH
cord-300796-rmjv56ia	290	26	terminal	terminal	JJ
cord-300796-rmjv56ia	290	27	part	part	NN
cord-300796-rmjv56ia	290	28	of	of	IN
cord-300796-rmjv56ia	290	29	the	the	DT
cord-300796-rmjv56ia	290	30	p62	p62	NN
cord-300796-rmjv56ia	290	31	segment	segment	NN
cord-300796-rmjv56ia	290	32	contains	contain	VBZ
cord-300796-rmjv56ia	290	33	a	a	DT
cord-300796-rmjv56ia	290	34	high	high	JJ
cord-300796-rmjv56ia	290	35	number	number	NN
cord-300796-rmjv56ia	290	36	of	of	IN
cord-300796-rmjv56ia	290	37	charged	charge	VBN
cord-300796-rmjv56ia	290	38	residues	residue	NNS
cord-300796-rmjv56ia	290	39	.	.	.
cord-300796-rmjv56ia	291	1	As	as	IN
cord-300796-rmjv56ia	291	2	shown	show	VBN
cord-300796-rmjv56ia	291	3	in	in	IN
cord-300796-rmjv56ia	291	4	Fig	Fig	NNP
cord-300796-rmjv56ia	291	5	.	.	.
cord-300796-rmjv56ia	292	1	7	7	CD
cord-300796-rmjv56ia	293	1	these	these	DT
cord-300796-rmjv56ia	293	2	features	feature	NNS
cord-300796-rmjv56ia	293	3	are	be	VBP
cord-300796-rmjv56ia	293	4	conserved	conserve	VBN
cord-300796-rmjv56ia	293	5	in	in	IN
cord-300796-rmjv56ia	293	6	all	all	PDT
cord-300796-rmjv56ia	293	7	those	those	DT
cord-300796-rmjv56ia	293	8	alphaviruses	alphavirus	NNS
cord-300796-rmjv56ia	293	9	where	where	WRB
cord-300796-rmjv56ia	293	10	the	the	DT
cord-300796-rmjv56ia	293	11	p62	p62	NN
cord-300796-rmjv56ia	293	12	protein	protein	NN
cord-300796-rmjv56ia	293	13	has	have	VBZ
cord-300796-rmjv56ia	293	14	been	be	VBN
cord-300796-rmjv56ia	293	15	sequenced	sequence	VBN
cord-300796-rmjv56ia	293	16	.	.	.
cord-300796-rmjv56ia	294	1	Thus	thus	RB
cord-300796-rmjv56ia	294	2	,	,	,
cord-300796-rmjv56ia	294	3	we	-PRON-	PRP
cord-300796-rmjv56ia	294	4	find	find	VBP
cord-300796-rmjv56ia	294	5	the	the	DT
cord-300796-rmjv56ia	294	6	experimental	experimental	JJ
cord-300796-rmjv56ia	294	7	results	result	NNS
cord-300796-rmjv56ia	294	8	,	,	,
cord-300796-rmjv56ia	294	9	together	together	RB
cord-300796-rmjv56ia	294	10	with	with	IN
cord-300796-rmjv56ia	294	11	the	the	DT
cord-300796-rmjv56ia	294	12	structural	structural	JJ
cord-300796-rmjv56ia	294	13	considerations	consideration	NNS
cord-300796-rmjv56ia	294	14	discussed	discuss	VBN
cord-300796-rmjv56ia	294	15	above	above	RB
cord-300796-rmjv56ia	294	16	,	,	,
cord-300796-rmjv56ia	294	17	highly	highly	RB
cord-300796-rmjv56ia	294	18	indicative	indicative	JJ
cord-300796-rmjv56ia	294	19	that	that	IN
cord-300796-rmjv56ia	294	20	the	the	DT
cord-300796-rmjv56ia	294	21	16	16	CD
cord-300796-rmjv56ia	294	22	NH2terminal	NH2terminal	NNP
cord-300796-rmjv56ia	294	23	residues	residue	NNS
cord-300796-rmjv56ia	294	24	of	of	IN
cord-300796-rmjv56ia	294	25	p62	p62	NN
cord-300796-rmjv56ia	294	26	constitute	constitute	VBP
cord-300796-rmjv56ia	294	27	its	-PRON-	PRP$
cord-300796-rmjv56ia	294	28	signal	signal	NN
cord-300796-rmjv56ia	294	29	sequence	sequence	NN
cord-300796-rmjv56ia	294	30	.	.	.
cord-300796-rmjv56ia	295	1	Eventually	eventually	RB
cord-300796-rmjv56ia	295	2	,	,	,
cord-300796-rmjv56ia	295	3	the	the	DT
cord-300796-rmjv56ia	295	4	signal	signal	JJ
cord-300796-rmjv56ia	295	5	sequence	sequence	NN
cord-300796-rmjv56ia	295	6	of	of	IN
cord-300796-rmjv56ia	295	7	the	the	DT
cord-300796-rmjv56ia	295	8	p62	p62	NN
cord-300796-rmjv56ia	295	9	protein	protein	NN
cord-300796-rmjv56ia	295	10	becomes	become	VBZ
cord-300796-rmjv56ia	295	11	translocated	translocate	VBN
cord-300796-rmjv56ia	295	12	across	across	IN
cord-300796-rmjv56ia	295	13	the	the	DT
cord-300796-rmjv56ia	295	14	membrane	membrane	NN
cord-300796-rmjv56ia	295	15	of	of	IN
cord-300796-rmjv56ia	295	16	the	the	DT
cord-300796-rmjv56ia	295	17	ER	ER	NNP
cord-300796-rmjv56ia	295	18	into	into	IN
cord-300796-rmjv56ia	295	19	its	-PRON-	PRP$
cord-300796-rmjv56ia	295	20	lumenal	lumenal	JJ
cord-300796-rmjv56ia	295	21	space	space	NN
cord-300796-rmjv56ia	295	22	.	.	.
cord-300796-rmjv56ia	296	1	In	in	IN
cord-300796-rmjv56ia	296	2	here	here	RB
cord-300796-rmjv56ia	296	3	it	-PRON-	PRP
cord-300796-rmjv56ia	296	4	is	be	VBZ
cord-300796-rmjv56ia	296	5	found	find	VBN
cord-300796-rmjv56ia	296	6	as	as	IN
cord-300796-rmjv56ia	296	7	a	a	DT
cord-300796-rmjv56ia	296	8	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	296	9	peptide	peptide	NN
cord-300796-rmjv56ia	296	10	which	which	WDT
cord-300796-rmjv56ia	296	11	is	be	VBZ
cord-300796-rmjv56ia	296	12	part	part	NN
cord-300796-rmjv56ia	296	13	of	of	IN
cord-300796-rmjv56ia	296	14	a	a	DT
cord-300796-rmjv56ia	296	15	66	66	CD
cord-300796-rmjv56ia	296	16	amino	amino	NN
cord-300796-rmjv56ia	296	17	acid	acid	NN
cord-300796-rmjv56ia	296	18	residues	residue	NNS
cord-300796-rmjv56ia	296	19	long	long	RB
cord-300796-rmjv56ia	296	20	"	"	``
cord-300796-rmjv56ia	296	21	pro	pro	JJ
cord-300796-rmjv56ia	296	22	-	-	JJ
cord-300796-rmjv56ia	296	23	piece	piece	NN
cord-300796-rmjv56ia	296	24	of	of	IN
cord-300796-rmjv56ia	296	25	the	the	DT
cord-300796-rmjv56ia	296	26	p62	p62	NN
cord-300796-rmjv56ia	296	27	protein	protein	NN
cord-300796-rmjv56ia	296	28	.	.	.
cord-300796-rmjv56ia	297	1	This	this	DT
cord-300796-rmjv56ia	297	2	pro	pro	JJ
cord-300796-rmjv56ia	297	3	-	-	JJ
cord-300796-rmjv56ia	297	4	peptide	peptide	JJ
cord-300796-rmjv56ia	297	5	,	,	,
cord-300796-rmjv56ia	297	6	called	call	VBN
cord-300796-rmjv56ia	297	7	E3	E3	NNP
cord-300796-rmjv56ia	297	8	,	,	,
cord-300796-rmjv56ia	297	9	is	be	VBZ
cord-300796-rmjv56ia	297	10	cleaved	cleave	VBN
cord-300796-rmjv56ia	297	11	at	at	IN
cord-300796-rmjv56ia	297	12	a	a	DT
cord-300796-rmjv56ia	297	13	late	late	JJ
cord-300796-rmjv56ia	297	14	stage	stage	NN
cord-300796-rmjv56ia	297	15	during	during	IN
cord-300796-rmjv56ia	297	16	virus	virus	NN
cord-300796-rmjv56ia	297	17	assembly	assembly	NN
cord-300796-rmjv56ia	297	18	(	(	-LRB-
cord-300796-rmjv56ia	297	19	de	de	IN
cord-300796-rmjv56ia	297	20	Curtis	Curtis	NNP
cord-300796-rmjv56ia	297	21	and	and	CC
cord-300796-rmjv56ia	297	22	Simons	Simons	NNP
cord-300796-rmjv56ia	297	23	,	,	,
cord-300796-rmjv56ia	297	24	1988	1988	CD
cord-300796-rmjv56ia	297	25	)	)	-RRB-
cord-300796-rmjv56ia	297	26	and	and	CC
cord-300796-rmjv56ia	297	27	is	be	VBZ
cord-300796-rmjv56ia	297	28	then	then	RB
cord-300796-rmjv56ia	297	29	either	either	RB
cord-300796-rmjv56ia	297	30	released	release	VBN
cord-300796-rmjv56ia	297	31	into	into	IN
cord-300796-rmjv56ia	297	32	the	the	DT
cord-300796-rmjv56ia	297	33	extracellular	extracellular	JJ
cord-300796-rmjv56ia	297	34	medium	medium	NN
cord-300796-rmjv56ia	297	35	as	as	IN
cord-300796-rmjv56ia	297	36	a	a	DT
cord-300796-rmjv56ia	297	37	soluble	soluble	JJ
cord-300796-rmjv56ia	297	38	protein	protein	NN
cord-300796-rmjv56ia	297	39	(	(	-LRB-
cord-300796-rmjv56ia	297	40	Sinbis	Sinbis	NNP
cord-300796-rmjv56ia	297	41	virus	virus	NN
cord-300796-rmjv56ia	297	42	)	)	-RRB-
cord-300796-rmjv56ia	297	43	or	or	CC
cord-300796-rmjv56ia	297	44	remains	remain	VBZ
cord-300796-rmjv56ia	297	45	as	as	IN
cord-300796-rmjv56ia	297	46	a	a	DT
cord-300796-rmjv56ia	297	47	peripheral	peripheral	JJ
cord-300796-rmjv56ia	297	48	protein	protein	NN
cord-300796-rmjv56ia	297	49	subunit	subunit	NN
cord-300796-rmjv56ia	297	50	on	on	IN
cord-300796-rmjv56ia	297	51	the	the	DT
cord-300796-rmjv56ia	297	52	virus	virus	NN
cord-300796-rmjv56ia	297	53	spike	spike	NN
cord-300796-rmjv56ia	297	54	(	(	-LRB-
cord-300796-rmjv56ia	297	55	SFV	sfv	NN
cord-300796-rmjv56ia	297	56	)	)	-RRB-
cord-300796-rmjv56ia	298	1	(	(	-LRB-
cord-300796-rmjv56ia	298	2	Garoff	Garoff	NNP
cord-300796-rmjv56ia	298	3	et	et	FW
cord-300796-rmjv56ia	298	4	al	al	NNP
cord-300796-rmjv56ia	298	5	.	.	NNP
cord-300796-rmjv56ia	298	6	,	,	,
cord-300796-rmjv56ia	298	7	1982	1982	CD
cord-300796-rmjv56ia	299	1	;	;	:
cord-300796-rmjv56ia	299	2	Mayne	Mayne	NNP
cord-300796-rmjv56ia	299	3	et	et	FW
cord-300796-rmjv56ia	299	4	al	al	NNP
cord-300796-rmjv56ia	299	5	.	.	NNP
cord-300796-rmjv56ia	299	6	,	,	,
cord-300796-rmjv56ia	299	7	1984	1984	CD
cord-300796-rmjv56ia	299	8	)	)	-RRB-
cord-300796-rmjv56ia	299	9	.	.	.
cord-300796-rmjv56ia	300	1	Our	-PRON-	PRP$
cord-300796-rmjv56ia	300	2	present	present	JJ
cord-300796-rmjv56ia	300	3	tests	test	NNS
cord-300796-rmjv56ia	300	4	of	of	IN
cord-300796-rmjv56ia	300	5	the	the	DT
cord-300796-rmjv56ia	300	6	p62-globin	p62-globin	CD
cord-300796-rmjv56ia	300	7	and	and	CC
cord-300796-rmjv56ia	300	8	p62	p62	VBG
cord-300796-rmjv56ia	300	9	dhfr	dhfr	NN
cord-300796-rmjv56ia	300	10	hybrids	hybrid	NNS
cord-300796-rmjv56ia	300	11	in	in	IN
cord-300796-rmjv56ia	300	12	the	the	DT
cord-300796-rmjv56ia	300	13	high	high	JJ
cord-300796-rmjv56ia	300	14	pH	pH	NNP
cord-300796-rmjv56ia	300	15	wash	wash	NN
cord-300796-rmjv56ia	300	16	assay	assay	NN
cord-300796-rmjv56ia	300	17	of	of	IN
cord-300796-rmjv56ia	300	18	membrane	membrane	NN
cord-300796-rmjv56ia	300	19	supplemented	supplement	VBN
cord-300796-rmjv56ia	300	20	in	in	IN
cord-300796-rmjv56ia	300	21	vitro	vitro	FW
cord-300796-rmjv56ia	300	22	mixtures	mixture	NNS
cord-300796-rmjv56ia	300	23	also	also	RB
cord-300796-rmjv56ia	300	24	support	support	VBP
cord-300796-rmjv56ia	300	25	the	the	DT
cord-300796-rmjv56ia	300	26	notion	notion	NN
cord-300796-rmjv56ia	300	27	that	that	IN
cord-300796-rmjv56ia	300	28	the	the	DT
cord-300796-rmjv56ia	300	29	p62	p62	NN
cord-300796-rmjv56ia	300	30	signal	signal	NN
cord-300796-rmjv56ia	300	31	sequence	sequence	NN
cord-300796-rmjv56ia	300	32	does	do	VBZ
cord-300796-rmjv56ia	300	33	not	not	RB
cord-300796-rmjv56ia	300	34	remain	remain	VB
cord-300796-rmjv56ia	300	35	bound	bound	JJ
cord-300796-rmjv56ia	300	36	to	to	IN
cord-300796-rmjv56ia	300	37	the	the	DT
cord-300796-rmjv56ia	300	38	membrane	membrane	NN
cord-300796-rmjv56ia	300	39	where	where	WRB
cord-300796-rmjv56ia	300	40	it	-PRON-	PRP
cord-300796-rmjv56ia	300	41	has	have	VBZ
cord-300796-rmjv56ia	300	42	exerted	exert	VBN
cord-300796-rmjv56ia	300	43	its	-PRON-	PRP$
cord-300796-rmjv56ia	300	44	function	function	NN
cord-300796-rmjv56ia	300	45	as	as	IN
cord-300796-rmjv56ia	300	46	a	a	DT
cord-300796-rmjv56ia	300	47	translocation	translocation	NN
cord-300796-rmjv56ia	300	48	signal	signal	NN
cord-300796-rmjv56ia	300	49	.	.	.
cord-300796-rmjv56ia	301	1	In	in	IN
cord-300796-rmjv56ia	301	2	this	this	DT
cord-300796-rmjv56ia	301	3	work	work	NN
cord-300796-rmjv56ia	301	4	we	-PRON-	PRP
cord-300796-rmjv56ia	301	5	like	like	VBP
cord-300796-rmjv56ia	301	6	to	to	TO
cord-300796-rmjv56ia	301	7	use	use	VB
cord-300796-rmjv56ia	301	8	the	the	DT
cord-300796-rmjv56ia	301	9	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	301	10	event	event	NN
cord-300796-rmjv56ia	301	11	at	at	IN
cord-300796-rmjv56ia	301	12	Asn,3	asn,3	NN
cord-300796-rmjv56ia	301	13	of	of	IN
cord-300796-rmjv56ia	301	14	the	the	DT
cord-300796-rmjv56ia	301	15	signal	signal	JJ
cord-300796-rmjv56ia	301	16	sequence	sequence	NN
cord-300796-rmjv56ia	301	17	to	to	TO
cord-300796-rmjv56ia	301	18	mark	mark	VB
cord-300796-rmjv56ia	301	19	the	the	DT
cord-300796-rmjv56ia	301	20	time	time	NN
cord-300796-rmjv56ia	301	21	point	point	NN
cord-300796-rmjv56ia	301	22	when	when	WRB
cord-300796-rmjv56ia	301	23	the	the	DT
cord-300796-rmjv56ia	301	24	latter	latter	NN
cord-300796-rmjv56ia	301	25	becomes	become	VBZ
cord-300796-rmjv56ia	301	26	released	release	VBN
cord-300796-rmjv56ia	301	27	into	into	IN
cord-300796-rmjv56ia	301	28	the	the	DT
cord-300796-rmjv56ia	301	29	lumen	lumen	NN
cord-300796-rmjv56ia	301	30	of	of	IN
cord-300796-rmjv56ia	301	31	the	the	DT
cord-300796-rmjv56ia	301	32	ER	ER	NNP
cord-300796-rmjv56ia	301	33	.	.	.
cord-300796-rmjv56ia	302	1	The	the	DT
cord-300796-rmjv56ia	302	2	crucial	crucial	JJ
cord-300796-rmjv56ia	302	3	question	question	NN
cord-300796-rmjv56ia	302	4	then	then	RB
cord-300796-rmjv56ia	302	5	becomes	become	VBZ
cord-300796-rmjv56ia	302	6	whether	whether	IN
cord-300796-rmjv56ia	302	7	it	-PRON-	PRP
cord-300796-rmjv56ia	302	8	is	be	VBZ
cord-300796-rmjv56ia	302	9	reasonable	reasonable	JJ
cord-300796-rmjv56ia	302	10	to	to	TO
cord-300796-rmjv56ia	302	11	assume	assume	VB
cord-300796-rmjv56ia	302	12	that	that	IN
cord-300796-rmjv56ia	302	13	the	the	DT
cord-300796-rmjv56ia	302	14	signal	signal	NN
cord-300796-rmjv56ia	302	15	peptide	peptide	NN
cord-300796-rmjv56ia	302	16	has	have	VBZ
cord-300796-rmjv56ia	302	17	to	to	TO
cord-300796-rmjv56ia	302	18	be	be	VB
cord-300796-rmjv56ia	302	19	released	release	VBN
cord-300796-rmjv56ia	302	20	from	from	IN
cord-300796-rmjv56ia	302	21	the	the	DT
cord-300796-rmjv56ia	302	22	ER	ER	NNP
cord-300796-rmjv56ia	302	23	membrane	membrane	NN
cord-300796-rmjv56ia	302	24	before	before	IN
cord-300796-rmjv56ia	302	25	it	-PRON-	PRP
cord-300796-rmjv56ia	302	26	can	can	MD
cord-300796-rmjv56ia	302	27	become	become	VB
cord-300796-rmjv56ia	302	28	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	302	29	.	.	.
cord-300796-rmjv56ia	303	1	To	to	TO
cord-300796-rmjv56ia	303	2	answer	answer	VB
cord-300796-rmjv56ia	303	3	this	this	DT
cord-300796-rmjv56ia	303	4	question	question	NN
cord-300796-rmjv56ia	303	5	we	-PRON-	PRP
cord-300796-rmjv56ia	303	6	have	have	VBP
cord-300796-rmjv56ia	303	7	to	to	TO
cord-300796-rmjv56ia	303	8	consider	consider	VB
cord-300796-rmjv56ia	303	9	what	what	WP
cord-300796-rmjv56ia	303	10	is	be	VBZ
cord-300796-rmjv56ia	303	11	known	know	VBN
cord-300796-rmjv56ia	303	12	about	about	IN
cord-300796-rmjv56ia	303	13	the	the	DT
cord-300796-rmjv56ia	303	14	topology	topology	NN
cord-300796-rmjv56ia	303	15	of	of	IN
cord-300796-rmjv56ia	303	16	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	303	17	as	as	RB
cord-300796-rmjv56ia	303	18	well	well	RB
cord-300796-rmjv56ia	303	19	as	as	IN
cord-300796-rmjv56ia	303	20	the	the	DT
cord-300796-rmjv56ia	303	21	way	way	NN
cord-300796-rmjv56ia	303	22	by	by	IN
cord-300796-rmjv56ia	303	23	which	which	WDT
cord-300796-rmjv56ia	303	24	the	the	DT
cord-300796-rmjv56ia	303	25	p62	p62	NN
cord-300796-rmjv56ia	303	26	signal	signal	NN
cord-300796-rmjv56ia	303	27	might	may	MD
cord-300796-rmjv56ia	303	28	interact	interact	VB
cord-300796-rmjv56ia	303	29	with	with	IN
cord-300796-rmjv56ia	303	30	the	the	DT
cord-300796-rmjv56ia	303	31	ER	ER	NNP
cord-300796-rmjv56ia	303	32	membrane	membrane	NN
cord-300796-rmjv56ia	303	33	.	.	.
cord-300796-rmjv56ia	304	1	Today	today	NN
cord-300796-rmjv56ia	304	2	there	there	EX
cord-300796-rmjv56ia	304	3	is	be	VBZ
cord-300796-rmjv56ia	304	4	no	no	DT
cord-300796-rmjv56ia	304	5	exact	exact	JJ
cord-300796-rmjv56ia	304	6	information	information	NN
cord-300796-rmjv56ia	304	7	about	about	IN
cord-300796-rmjv56ia	304	8	how	how	WRB
cord-300796-rmjv56ia	304	9	a	a	DT
cord-300796-rmjv56ia	304	10	signal	signal	JJ
cord-300796-rmjv56ia	304	11	sequence	sequence	NN
cord-300796-rmjv56ia	304	12	might	may	MD
cord-300796-rmjv56ia	304	13	be	be	VB
cord-300796-rmjv56ia	304	14	inserted	insert	VBN
cord-300796-rmjv56ia	304	15	into	into	IN
cord-300796-rmjv56ia	304	16	the	the	DT
cord-300796-rmjv56ia	304	17	ER	ER	NNP
cord-300796-rmjv56ia	304	18	membrane	membrane	NN
cord-300796-rmjv56ia	304	19	when	when	WRB
cord-300796-rmjv56ia	304	20	exerting	exert	VBG
cord-300796-rmjv56ia	304	21	its	-PRON-	PRP$
cord-300796-rmjv56ia	304	22	function	function	NN
cord-300796-rmjv56ia	304	23	in	in	IN
cord-300796-rmjv56ia	304	24	chain	chain	NN
cord-300796-rmjv56ia	304	25	translocation	translocation	NN
cord-300796-rmjv56ia	304	26	.	.	.
cord-300796-rmjv56ia	305	1	However	however	RB
cord-300796-rmjv56ia	305	2	,	,	,
cord-300796-rmjv56ia	305	3	the	the	DT
cord-300796-rmjv56ia	305	4	typical	typical	JJ
cord-300796-rmjv56ia	305	5	cytoplasmic	cytoplasmic	JJ
cord-300796-rmjv56ia	305	6	orientation	orientation	NN
cord-300796-rmjv56ia	305	7	of	of	IN
cord-300796-rmjv56ia	305	8	the	the	DT
cord-300796-rmjv56ia	305	9	NH2-termini	nh2-termini	NN
cord-300796-rmjv56ia	305	10	of	of	IN
cord-300796-rmjv56ia	305	11	membrane	membrane	NN
cord-300796-rmjv56ia	305	12	protein	protein	NN
cord-300796-rmjv56ia	305	13	chains	chain	NNS
cord-300796-rmjv56ia	305	14	carrying	carry	VBG
cord-300796-rmjv56ia	305	15	a	a	DT
cord-300796-rmjv56ia	305	16	combined	combine	VBN
cord-300796-rmjv56ia	305	17	signal	signal	NN
cord-300796-rmjv56ia	305	18	sequence	sequence	NN
cord-300796-rmjv56ia	305	19	-	-	HYPH
cord-300796-rmjv56ia	305	20	anchoring	anchor	VBG
cord-300796-rmjv56ia	305	21	peptide	peptide	NN
cord-300796-rmjv56ia	305	22	suggests	suggest	VBZ
cord-300796-rmjv56ia	305	23	that	that	IN
cord-300796-rmjv56ia	305	24	signal	signal	NN
cord-300796-rmjv56ia	305	25	sequences	sequence	NNS
cord-300796-rmjv56ia	305	26	in	in	IN
cord-300796-rmjv56ia	305	27	general	general	JJ
cord-300796-rmjv56ia	305	28	might	may	MD
cord-300796-rmjv56ia	305	29	direct	direct	VB
cord-300796-rmjv56ia	305	30	their	-PRON-	PRP$
cord-300796-rmjv56ia	305	31	function	function	NN
cord-300796-rmjv56ia	305	32	in	in	IN
cord-300796-rmjv56ia	305	33	translocation	translocation	NN
cord-300796-rmjv56ia	305	34	through	through	IN
cord-300796-rmjv56ia	305	35	the	the	DT
cord-300796-rmjv56ia	305	36	insertion	insertion	NN
cord-300796-rmjv56ia	305	37	of	of	IN
cord-300796-rmjv56ia	305	38	their	-PRON-	PRP$
cord-300796-rmjv56ia	305	39	hydrophobic	hydrophobic	JJ
cord-300796-rmjv56ia	305	40	and	and	CC
cord-300796-rmjv56ia	305	41	uncharged	uncharged	JJ
cord-300796-rmjv56ia	305	42	stretch	stretch	NN
cord-300796-rmjv56ia	305	43	of	of	IN
cord-300796-rmjv56ia	305	44	amino	amino	NN
cord-300796-rmjv56ia	305	45	acid	acid	NN
cord-300796-rmjv56ia	305	46	residues	residue	NNS
cord-300796-rmjv56ia	305	47	into	into	IN
cord-300796-rmjv56ia	305	48	the	the	DT
cord-300796-rmjv56ia	305	49	membrane	membrane	NN
cord-300796-rmjv56ia	305	50	in	in	IN
cord-300796-rmjv56ia	305	51	such	such	PDT
cord-300796-rmjv56ia	305	52	an	an	DT
cord-300796-rmjv56ia	305	53	orientation	orientation	NN
cord-300796-rmjv56ia	305	54	that	that	WDT
cord-300796-rmjv56ia	305	55	the	the	DT
cord-300796-rmjv56ia	305	56	NHEterminus	NHEterminus	NNP
cord-300796-rmjv56ia	305	57	of	of	IN
cord-300796-rmjv56ia	305	58	the	the	DT
cord-300796-rmjv56ia	305	59	signal	signal	NN
cord-300796-rmjv56ia	305	60	remains	remain	VBZ
cord-300796-rmjv56ia	305	61	on	on	IN
cord-300796-rmjv56ia	305	62	the	the	DT
cord-300796-rmjv56ia	305	63	outside	outside	NN
cord-300796-rmjv56ia	305	64	of	of	IN
cord-300796-rmjv56ia	305	65	the	the	DT
cord-300796-rmjv56ia	305	66	ER	ER	NNP
cord-300796-rmjv56ia	305	67	mem-	mem-	NN
cord-300796-rmjv56ia	306	1	(	(	-LRB-
cord-300796-rmjv56ia	306	2	Garoff	Garoff	NNP
cord-300796-rmjv56ia	306	3	et	et	FW
cord-300796-rmjv56ia	306	4	al	al	NNP
cord-300796-rmjv56ia	306	5	.	.	NNP
cord-300796-rmjv56ia	306	6	,	,	,
cord-300796-rmjv56ia	306	7	1980	1980	CD
cord-300796-rmjv56ia	306	8	;	;	:
cord-300796-rmjv56ia	306	9	Rice	Rice	NNP
cord-300796-rmjv56ia	306	10	and	and	CC
cord-300796-rmjv56ia	306	11	Strauss	Strauss	NNP
cord-300796-rmjv56ia	306	12	,	,	,
cord-300796-rmjv56ia	306	13	1981	1981	CD
cord-300796-rmjv56ia	306	14	;	;	:
cord-300796-rmjv56ia	306	15	Dalgarno	Dalgarno	NNP
cord-300796-rmjv56ia	306	16	et	et	NNP
cord-300796-rmjv56ia	306	17	al	al	NNP
cord-300796-rmjv56ia	306	18	.	.	NNP
cord-300796-rmjv56ia	306	19	,	,	,
cord-300796-rmjv56ia	306	20	1983	1983	CD
cord-300796-rmjv56ia	306	21	;	;	:
cord-300796-rmjv56ia	306	22	Kinney	Kinney	NNP
cord-300796-rmjv56ia	306	23	et	et	NNP
cord-300796-rmjv56ia	306	24	al	al	NNP
cord-300796-rmjv56ia	306	25	.	.	NNP
cord-300796-rmjv56ia	306	26	,	,	,
cord-300796-rmjv56ia	306	27	1986	1986	CD
cord-300796-rmjv56ia	306	28	;	;	:
cord-300796-rmjv56ia	306	29	Chang	Chang	NNP
cord-300796-rmjv56ia	306	30	and	and	CC
cord-300796-rmjv56ia	306	31	Trent	Trent	NNP
cord-300796-rmjv56ia	306	32	,	,	,
cord-300796-rmjv56ia	306	33	1987	1987	CD
cord-300796-rmjv56ia	306	34	)	)	-RRB-
cord-300796-rmjv56ia	306	35	.	.	.
cord-300796-rmjv56ia	307	1	Amino	amino	NN
cord-300796-rmjv56ia	307	2	acid	acid	NN
cord-300796-rmjv56ia	307	3	residues	residue	NNS
cord-300796-rmjv56ia	307	4	are	be	VBP
cord-300796-rmjv56ia	307	5	given	give	VBN
cord-300796-rmjv56ia	307	6	using	use	VBG
cord-300796-rmjv56ia	307	7	the	the	DT
cord-300796-rmjv56ia	307	8	one	one	CD
cord-300796-rmjv56ia	307	9	letter	letter	NN
cord-300796-rmjv56ia	307	10	code	code	NN
cord-300796-rmjv56ia	307	11	and	and	CC
cord-300796-rmjv56ia	307	12	they	-PRON-	PRP
cord-300796-rmjv56ia	307	13	are	be	VBP
cord-300796-rmjv56ia	307	14	numbered	number	VBN
cord-300796-rmjv56ia	307	15	from	from	IN
cord-300796-rmjv56ia	307	16	the	the	DT
cord-300796-rmjv56ia	307	17	NH2-towards	nh2-toward	NNS
cord-300796-rmjv56ia	307	18	the	the	DT
cord-300796-rmjv56ia	307	19	COOH	COOH	NNP
cord-300796-rmjv56ia	307	20	-	-	HYPH
cord-300796-rmjv56ia	307	21	terminus	terminus	NN
cord-300796-rmjv56ia	307	22	.	.	.
cord-300796-rmjv56ia	308	1	The	the	DT
cord-300796-rmjv56ia	308	2	boxes	box	NNS
cord-300796-rmjv56ia	308	3	indicate	indicate	VBP
cord-300796-rmjv56ia	308	4	that	that	IN
cord-300796-rmjv56ia	308	5	region	region	NN
cord-300796-rmjv56ia	308	6	in	in	IN
cord-300796-rmjv56ia	308	7	each	each	DT
cord-300796-rmjv56ia	308	8	sequence	sequence	NN
cord-300796-rmjv56ia	308	9	which	which	WDT
cord-300796-rmjv56ia	308	10	best	good	JJS
cord-300796-rmjv56ia	308	11	fulfills	fulfill	VBZ
cord-300796-rmjv56ia	308	12	the	the	DT
cord-300796-rmjv56ia	308	13	consensus	consensus	NN
cord-300796-rmjv56ia	308	14	features	feature	NNS
cord-300796-rmjv56ia	308	15	of	of	IN
cord-300796-rmjv56ia	308	16	a	a	DT
cord-300796-rmjv56ia	308	17	signal	signal	JJ
cord-300796-rmjv56ia	308	18	sequence	sequence	NN
cord-300796-rmjv56ia	308	19	(	(	-LRB-
cord-300796-rmjv56ia	308	20	the	the	DT
cord-300796-rmjv56ia	308	21	uncharged	uncharged	JJ
cord-300796-rmjv56ia	308	22	and	and	CC
cord-300796-rmjv56ia	308	23	hydrophobic	hydrophobic	JJ
cord-300796-rmjv56ia	308	24	region	region	NN
cord-300796-rmjv56ia	308	25	)	)	-RRB-
cord-300796-rmjv56ia	308	26	.	.	.
cord-300796-rmjv56ia	309	1	The	the	DT
cord-300796-rmjv56ia	309	2	*	*	NFP
cord-300796-rmjv56ia	309	3	symbols	symbol	NNS
cord-300796-rmjv56ia	309	4	represent	represent	VBP
cord-300796-rmjv56ia	309	5	attachment	attachment	NN
cord-300796-rmjv56ia	309	6	sites	site	NNS
cord-300796-rmjv56ia	309	7	for	for	IN
cord-300796-rmjv56ia	309	8	oligosaccharide	oligosaccharide	NN
cord-300796-rmjv56ia	309	9	and	and	CC
cord-300796-rmjv56ia	309	10	the	the	DT
cord-300796-rmjv56ia	309	11	(	(	-LRB-
cord-300796-rmjv56ia	309	12	+	+	NN
cord-300796-rmjv56ia	309	13	)	)	-RRB-
cord-300796-rmjv56ia	309	14	and	and	CC
cord-300796-rmjv56ia	309	15	(	(	-LRB-
cord-300796-rmjv56ia	309	16	-	-	:
cord-300796-rmjv56ia	309	17	)	)	-RRB-
cord-300796-rmjv56ia	309	18	the	the	DT
cord-300796-rmjv56ia	309	19	presence	presence	NN
cord-300796-rmjv56ia	309	20	of	of	IN
cord-300796-rmjv56ia	309	21	a	a	DT
cord-300796-rmjv56ia	309	22	charged	charge	VBN
cord-300796-rmjv56ia	309	23	amino	amino	NN
cord-300796-rmjv56ia	309	24	acid	acid	NN
cord-300796-rmjv56ia	309	25	side	side	NN
cord-300796-rmjv56ia	309	26	chain	chain	NN
cord-300796-rmjv56ia	309	27	.	.	.
cord-300796-rmjv56ia	310	1	Proline	proline	NN
cord-300796-rmjv56ia	310	2	residues	residue	NNS
cord-300796-rmjv56ia	310	3	are	be	VBP
cord-300796-rmjv56ia	310	4	labeled	label	VBN
cord-300796-rmjv56ia	310	5	with	with	IN
cord-300796-rmjv56ia	310	6	a	a	DT
cord-300796-rmjv56ia	310	7	dot	dot	NN
cord-300796-rmjv56ia	310	8	.	.	.
cord-300796-rmjv56ia	311	1	The	the	DT
cord-300796-rmjv56ia	311	2	sequences	sequence	NNS
cord-300796-rmjv56ia	311	3	are	be	VBP
cord-300796-rmjv56ia	311	4	aligned	align	VBN
cord-300796-rmjv56ia	311	5	according	accord	VBG
cord-300796-rmjv56ia	311	6	to	to	IN
cord-300796-rmjv56ia	311	7	maximum	maximum	JJ
cord-300796-rmjv56ia	311	8	amino	amino	NN
cord-300796-rmjv56ia	311	9	acid	acid	NN
cord-300796-rmjv56ia	311	10	sequence	sequence	NN
cord-300796-rmjv56ia	311	11	homology	homology	NN
cord-300796-rmjv56ia	311	12	.	.	.
cord-300796-rmjv56ia	312	1	brane	brane	NNP
cord-300796-rmjv56ia	312	2	(	(	-LRB-
cord-300796-rmjv56ia	312	3	Bos	Bos	NNP
cord-300796-rmjv56ia	312	4	et	et	NNP
cord-300796-rmjv56ia	312	5	al	al	NNP
cord-300796-rmjv56ia	312	6	.	.	NNP
cord-300796-rmjv56ia	312	7	,	,	,
cord-300796-rmjv56ia	312	8	1984	1984	CD
cord-300796-rmjv56ia	312	9	;	;	.
cord-300796-rmjv56ia	313	1	Lipp	Lipp	NNP
cord-300796-rmjv56ia	313	2	and	and	CC
cord-300796-rmjv56ia	313	3	Dobberstein	Dobberstein	NNP
cord-300796-rmjv56ia	313	4	,	,	,
cord-300796-rmjv56ia	313	5	1986	1986	CD
cord-300796-rmjv56ia	313	6	;	;	:
cord-300796-rmjv56ia	313	7	Spiess	Spiess	NNP
cord-300796-rmjv56ia	313	8	and	and	CC
cord-300796-rmjv56ia	313	9	Lodish	Lodish	NNP
cord-300796-rmjv56ia	313	10	,	,	,
cord-300796-rmjv56ia	313	11	1986	1986	CD
cord-300796-rmjv56ia	313	12	;	;	:
cord-300796-rmjv56ia	313	13	Zerial	Zerial	NNP
cord-300796-rmjv56ia	313	14	et	et	FW
cord-300796-rmjv56ia	313	15	al	al	NNP
cord-300796-rmjv56ia	313	16	.	.	NNP
cord-300796-rmjv56ia	313	17	,	,	,
cord-300796-rmjv56ia	313	18	1986	1986	CD
cord-300796-rmjv56ia	313	19	;	;	:
cord-300796-rmjv56ia	313	20	see	see	VB
cord-300796-rmjv56ia	313	21	also	also	RB
cord-300796-rmjv56ia	313	22	Shaw	Shaw	NNP
cord-300796-rmjv56ia	313	23	et	et	NNP
cord-300796-rmjv56ia	313	24	al	al	NNP
cord-300796-rmjv56ia	313	25	.	.	NNP
cord-300796-rmjv56ia	313	26	,	,	,
cord-300796-rmjv56ia	313	27	1988	1988	CD
cord-300796-rmjv56ia	313	28	)	)	-RRB-
cord-300796-rmjv56ia	313	29	.	.	.
cord-300796-rmjv56ia	314	1	In	in	IN
cord-300796-rmjv56ia	314	2	addition	addition	NN
cord-300796-rmjv56ia	314	3	,	,	,
cord-300796-rmjv56ia	314	4	it	-PRON-	PRP
cord-300796-rmjv56ia	314	5	is	be	VBZ
cord-300796-rmjv56ia	314	6	known	know	VBN
cord-300796-rmjv56ia	314	7	from	from	IN
cord-300796-rmjv56ia	314	8	physical	physical	JJ
cord-300796-rmjv56ia	314	9	studies	study	NNS
cord-300796-rmjv56ia	314	10	using	use	VBG
cord-300796-rmjv56ia	314	11	synthetic	synthetic	JJ
cord-300796-rmjv56ia	314	12	signal	signal	NN
cord-300796-rmjv56ia	314	13	peptides	peptide	NNS
cord-300796-rmjv56ia	314	14	and	and	CC
cord-300796-rmjv56ia	314	15	artificial	artificial	JJ
cord-300796-rmjv56ia	314	16	lipid	lipid	NN
cord-300796-rmjv56ia	314	17	membranes	membrane	NNS
cord-300796-rmjv56ia	314	18	that	that	WDT
cord-300796-rmjv56ia	314	19	the	the	DT
cord-300796-rmjv56ia	314	20	signal	signal	NN
cord-300796-rmjv56ia	314	21	peptides	peptide	NNS
cord-300796-rmjv56ia	314	22	readily	readily	RB
cord-300796-rmjv56ia	314	23	insert	insert	VBP
cord-300796-rmjv56ia	314	24	into	into	IN
cord-300796-rmjv56ia	314	25	the	the	DT
cord-300796-rmjv56ia	314	26	membrane	membrane	NN
cord-300796-rmjv56ia	314	27	and	and	CC
cord-300796-rmjv56ia	314	28	there	there	EX
cord-300796-rmjv56ia	314	29	obtain	obtain	VB
cord-300796-rmjv56ia	314	30	an	an	DT
cord-300796-rmjv56ia	314	31	alpha	alpha	JJ
cord-300796-rmjv56ia	314	32	-	-	HYPH
cord-300796-rmjv56ia	314	33	helical	helical	JJ
cord-300796-rmjv56ia	314	34	conformation	conformation	NN
cord-300796-rmjv56ia	315	1	(	(	-LRB-
cord-300796-rmjv56ia	315	2	Briggs	Briggs	NNP
cord-300796-rmjv56ia	315	3	et	et	NNP
cord-300796-rmjv56ia	315	4	al	al	NNP
cord-300796-rmjv56ia	315	5	.	.	NNP
cord-300796-rmjv56ia	315	6	,	,	,
cord-300796-rmjv56ia	315	7	1985	1985	CD
cord-300796-rmjv56ia	316	1	(	(	-LRB-
cord-300796-rmjv56ia	316	2	Briggs	Briggs	NNP
cord-300796-rmjv56ia	316	3	et	et	NNP
cord-300796-rmjv56ia	316	4	al	al	NNP
cord-300796-rmjv56ia	316	5	.	.	.
cord-300796-rmjv56ia	317	1	,	,	,
cord-300796-rmjv56ia	317	2	,	,	,
cord-300796-rmjv56ia	317	3	1986	1986	CD
cord-300796-rmjv56ia	317	4	Batenburg	Batenburg	NNP
cord-300796-rmjv56ia	317	5	et	et	NNP
cord-300796-rmjv56ia	317	6	al	al	NNP
cord-300796-rmjv56ia	317	7	.	.	NNP
cord-300796-rmjv56ia	317	8	,	,	,
cord-300796-rmjv56ia	317	9	1988	1988	CD
cord-300796-rmjv56ia	317	10	;	;	:
cord-300796-rmjv56ia	317	11	Cornell	Cornell	NNP
cord-300796-rmjv56ia	317	12	et	et	NNP
cord-300796-rmjv56ia	317	13	al	al	NNP
cord-300796-rmjv56ia	317	14	.	.	NNP
cord-300796-rmjv56ia	317	15	,	,	,
cord-300796-rmjv56ia	317	16	1989	1989	CD
cord-300796-rmjv56ia	317	17	)	)	-RRB-
cord-300796-rmjv56ia	317	18	.	.	.
cord-300796-rmjv56ia	318	1	If	if	IN
cord-300796-rmjv56ia	318	2	the	the	DT
cord-300796-rmjv56ia	318	3	p62	p62	NN
cord-300796-rmjv56ia	318	4	signal	signal	NN
cord-300796-rmjv56ia	318	5	sequence	sequence	NN
cord-300796-rmjv56ia	318	6	adapts	adapt	VBZ
cord-300796-rmjv56ia	318	7	such	such	PDT
cord-300796-rmjv56ia	318	8	an	an	DT
cord-300796-rmjv56ia	318	9	orientation	orientation	NN
cord-300796-rmjv56ia	318	10	and	and	CC
cord-300796-rmjv56ia	318	11	conformation	conformation	NN
cord-300796-rmjv56ia	318	12	in	in	IN
cord-300796-rmjv56ia	318	13	the	the	DT
cord-300796-rmjv56ia	318	14	ER	ER	NNP
cord-300796-rmjv56ia	318	15	membrane	membrane	NN
cord-300796-rmjv56ia	318	16	it	-PRON-	PRP
cord-300796-rmjv56ia	318	17	would	would	MD
cord-300796-rmjv56ia	318	18	mean	mean	VB
cord-300796-rmjv56ia	318	19	that	that	IN
cord-300796-rmjv56ia	318	20	the	the	DT
cord-300796-rmjv56ia	318	21	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	318	22	site	site	NN
cord-300796-rmjv56ia	318	23	at	at	IN
cord-300796-rmjv56ia	318	24	Asnt3	Asnt3	NNP
cord-300796-rmjv56ia	318	25	would	would	MD
cord-300796-rmjv56ia	318	26	locate	locate	VB
cord-300796-rmjv56ia	318	27	inside	inside	IN
cord-300796-rmjv56ia	318	28	the	the	DT
cord-300796-rmjv56ia	318	29	membrane	membrane	NN
cord-300796-rmjv56ia	318	30	(	(	-LRB-
cord-300796-rmjv56ia	318	31	von	von	NNP
cord-300796-rmjv56ia	318	32	Heijne	Heijne	NNP
cord-300796-rmjv56ia	318	33	,	,	,
cord-300796-rmjv56ia	318	34	1985	1985	CD
cord-300796-rmjv56ia	318	35	)	)	-RRB-
cord-300796-rmjv56ia	318	36	.	.	.
cord-300796-rmjv56ia	319	1	In	in	IN
cord-300796-rmjv56ia	319	2	this	this	DT
cord-300796-rmjv56ia	319	3	location	location	NN
cord-300796-rmjv56ia	319	4	the	the	DT
cord-300796-rmjv56ia	319	5	site	site	NN
cord-300796-rmjv56ia	319	6	can	can	MD
cord-300796-rmjv56ia	319	7	hardly	hardly	RB
cord-300796-rmjv56ia	319	8	be	be	VB
cord-300796-rmjv56ia	319	9	accessible	accessible	JJ
cord-300796-rmjv56ia	319	10	for	for	IN
cord-300796-rmjv56ia	319	11	the	the	DT
cord-300796-rmjv56ia	319	12	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	319	13	machinery	machinery	NN
cord-300796-rmjv56ia	319	14	.	.	.
cord-300796-rmjv56ia	320	1	(	(	-LRB-
cord-300796-rmjv56ia	320	2	Note	note	VB
cord-300796-rmjv56ia	320	3	that	that	IN
cord-300796-rmjv56ia	320	4	in	in	IN
cord-300796-rmjv56ia	320	5	the	the	DT
cord-300796-rmjv56ia	320	6	related	relate	VBN
cord-300796-rmjv56ia	320	7	Ross	Ross	NNP
cord-300796-rmjv56ia	320	8	River	River	NNP
cord-300796-rmjv56ia	320	9	virus	virus	NN
cord-300796-rmjv56ia	320	10	,	,	,
cord-300796-rmjv56ia	320	11	the	the	DT
cord-300796-rmjv56ia	320	12	Venezuelan	venezuelan	JJ
cord-300796-rmjv56ia	320	13	Equine	Equine	NNP
cord-300796-rmjv56ia	320	14	Encephalitis	Encephalitis	NNP
cord-300796-rmjv56ia	320	15	virus	virus	NN
cord-300796-rmjv56ia	320	16	and	and	CC
cord-300796-rmjv56ia	320	17	Eastern	eastern	JJ
cord-300796-rmjv56ia	320	18	Equine	Equine	NNP
cord-300796-rmjv56ia	320	19	Encephalitis	Encephalitis	NNP
cord-300796-rmjv56ia	320	20	virus	virus	NN
cord-300796-rmjv56ia	320	21	the	the	DT
cord-300796-rmjv56ia	320	22	corresponding	corresponding	JJ
cord-300796-rmjv56ia	320	23	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	320	24	site	site	NN
cord-300796-rmjv56ia	320	25	is	be	VBZ
cord-300796-rmjv56ia	320	26	even	even	RB
cord-300796-rmjv56ia	320	27	closer	close	JJR
cord-300796-rmjv56ia	320	28	towards	towards	IN
cord-300796-rmjv56ia	320	29	the	the	DT
cord-300796-rmjv56ia	320	30	NH2terminus	NH2terminus	NNP
cord-300796-rmjv56ia	320	31	,	,	,
cord-300796-rmjv56ia	320	32	that	that	DT
cord-300796-rmjv56ia	320	33	is	be	VBZ
cord-300796-rmjv56ia	320	34	at	at	IN
cord-300796-rmjv56ia	320	35	Asn	Asn	NNP
cord-300796-rmjv56ia	320	36	11	11	CD
cord-300796-rmjv56ia	320	37	,	,	,
cord-300796-rmjv56ia	320	38	see	see	VB
cord-300796-rmjv56ia	320	39	Fig	Fig	NNP
cord-300796-rmjv56ia	320	40	.	.	NNP
cord-300796-rmjv56ia	320	41	7	7	CD
cord-300796-rmjv56ia	320	42	.	.	.
cord-300796-rmjv56ia	320	43	)	)	-RRB-
cord-300796-rmjv56ia	321	1	According	accord	VBG
cord-300796-rmjv56ia	321	2	to	to	IN
cord-300796-rmjv56ia	321	3	several	several	JJ
cord-300796-rmjv56ia	321	4	recent	recent	JJ
cord-300796-rmjv56ia	321	5	studies	study	NNS
cord-300796-rmjv56ia	321	6	,	,	,
cord-300796-rmjv56ia	321	7	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	321	8	requires	require	VBZ
cord-300796-rmjv56ia	321	9	the	the	DT
cord-300796-rmjv56ia	321	10	exposure	exposure	NN
cord-300796-rmjv56ia	321	11	of	of	IN
cord-300796-rmjv56ia	321	12	the	the	DT
cord-300796-rmjv56ia	321	13	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	321	14	site	site	NN
cord-300796-rmjv56ia	321	15	in	in	IN
cord-300796-rmjv56ia	321	16	the	the	DT
cord-300796-rmjv56ia	321	17	lumen	lumen	NN
cord-300796-rmjv56ia	321	18	of	of	IN
cord-300796-rmjv56ia	321	19	ER	ER	NNP
cord-300796-rmjv56ia	321	20	.	.	.
cord-300796-rmjv56ia	322	1	Firstly	firstly	RB
cord-300796-rmjv56ia	322	2	,	,	,
cord-300796-rmjv56ia	322	3	it	-PRON-	PRP
cord-300796-rmjv56ia	322	4	has	have	VBZ
cord-300796-rmjv56ia	322	5	been	be	VBN
cord-300796-rmjv56ia	322	6	shown	show	VBN
cord-300796-rmjv56ia	322	7	that	that	IN
cord-300796-rmjv56ia	322	8	the	the	DT
cord-300796-rmjv56ia	322	9	binding	bind	VBG
cord-300796-rmjv56ia	322	10	protein	protein	NN
cord-300796-rmjv56ia	322	11	for	for	IN
cord-300796-rmjv56ia	322	12	the	the	DT
cord-300796-rmjv56ia	322	13	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	322	14	site	site	NN
cord-300796-rmjv56ia	322	15	of	of	IN
cord-300796-rmjv56ia	322	16	N	n	NN
cord-300796-rmjv56ia	322	17	-	-	HYPH
cord-300796-rmjv56ia	322	18	linked	link	VBN
cord-300796-rmjv56ia	322	19	oligosaccharides	oligosaccharide	NNS
cord-300796-rmjv56ia	322	20	is	be	VBZ
cord-300796-rmjv56ia	322	21	a	a	DT
cord-300796-rmjv56ia	322	22	lumenal	lumenal	JJ
cord-300796-rmjv56ia	322	23	57-kD	57-kd	CD
cord-300796-rmjv56ia	322	24	protein	protein	NN
cord-300796-rmjv56ia	322	25	of	of	IN
cord-300796-rmjv56ia	322	26	the	the	DT
cord-300796-rmjv56ia	322	27	ER	ER	NNP
cord-300796-rmjv56ia	322	28	(	(	-LRB-
cord-300796-rmjv56ia	322	29	Geetha	Geetha	NNP
cord-300796-rmjv56ia	322	30	-	-	HYPH
cord-300796-rmjv56ia	322	31	Habib	Habib	NNP
cord-300796-rmjv56ia	322	32	et	et	NNP
cord-300796-rmjv56ia	322	33	al	al	NNP
cord-300796-rmjv56ia	322	34	.	.	NNP
cord-300796-rmjv56ia	322	35	,	,	,
cord-300796-rmjv56ia	322	36	1988	1988	CD
cord-300796-rmjv56ia	322	37	)	)	-RRB-
cord-300796-rmjv56ia	322	38	.	.	.
cord-300796-rmjv56ia	323	1	Secondly	secondly	RB
cord-300796-rmjv56ia	323	2	,	,	,
cord-300796-rmjv56ia	323	3	one	one	CD
cord-300796-rmjv56ia	323	4	study	study	NN
cord-300796-rmjv56ia	323	5	with	with	IN
cord-300796-rmjv56ia	323	6	the	the	DT
cord-300796-rmjv56ia	323	7	asialoglycoprotein	asialoglycoprotein	NNP
cord-300796-rmjv56ia	323	8	receptor	receptor	NN
cord-300796-rmjv56ia	323	9	and	and	CC
cord-300796-rmjv56ia	323	10	another	another	DT
cord-300796-rmjv56ia	323	11	one	one	NN
cord-300796-rmjv56ia	323	12	with	with	IN
cord-300796-rmjv56ia	323	13	the	the	DT
cord-300796-rmjv56ia	323	14	Corona	Corona	NNP
cord-300796-rmjv56ia	323	15	virus	virus	NN
cord-300796-rmjv56ia	323	16	E1	e1	NN
cord-300796-rmjv56ia	323	17	membrane	membrane	NN
cord-300796-rmjv56ia	323	18	protein	protein	NN
cord-300796-rmjv56ia	323	19	demonstrate	demonstrate	VBP
cord-300796-rmjv56ia	323	20	that	that	IN
cord-300796-rmjv56ia	323	21	lumenally	lumenally	RB
cord-300796-rmjv56ia	323	22	oriented	orient	VBN
cord-300796-rmjv56ia	323	23	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	323	24	sites	site	NNS
cord-300796-rmjv56ia	323	25	are	be	VBP
cord-300796-rmjv56ia	323	26	not	not	RB
cord-300796-rmjv56ia	323	27	used	use	VBN
cord-300796-rmjv56ia	323	28	on	on	IN
cord-300796-rmjv56ia	323	29	transmembrane	transmembrane	NN
cord-300796-rmjv56ia	323	30	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	323	31	if	if	IN
cord-300796-rmjv56ia	323	32	they	-PRON-	PRP
cord-300796-rmjv56ia	323	33	locate	locate	VBP
cord-300796-rmjv56ia	323	34	very	very	RB
cord-300796-rmjv56ia	323	35	close	close	RB
cord-300796-rmjv56ia	323	36	to	to	IN
cord-300796-rmjv56ia	323	37	the	the	DT
cord-300796-rmjv56ia	323	38	membranebinding	membranebinding	NN
cord-300796-rmjv56ia	323	39	segments	segment	NNS
cord-300796-rmjv56ia	323	40	of	of	IN
cord-300796-rmjv56ia	323	41	the	the	DT
cord-300796-rmjv56ia	323	42	chains	chain	NNS
cord-300796-rmjv56ia	323	43	(	(	-LRB-
cord-300796-rmjv56ia	323	44	Mayer	Mayer	NNP
cord-300796-rmjv56ia	323	45	et	et	NNP
cord-300796-rmjv56ia	323	46	al	al	NNP
cord-300796-rmjv56ia	323	47	.	.	.
cord-300796-rmjv56ia	324	1	,	,	,
cord-300796-rmjv56ia	324	2	1988	1988	CD
cord-300796-rmjv56ia	324	3	;	;	:
cord-300796-rmjv56ia	324	4	Wessels	Wessels	NNP
cord-300796-rmjv56ia	324	5	and	and	CC
cord-300796-rmjv56ia	324	6	Spiess	Spiess	NNP
cord-300796-rmjv56ia	324	7	,	,	,
cord-300796-rmjv56ia	324	8	1988	1988	CD
cord-300796-rmjv56ia	324	9	)	)	-RRB-
cord-300796-rmjv56ia	324	10	.	.	.
cord-300796-rmjv56ia	325	1	In	in	IN
cord-300796-rmjv56ia	325	2	the	the	DT
cord-300796-rmjv56ia	325	3	case	case	NN
cord-300796-rmjv56ia	325	4	of	of	IN
cord-300796-rmjv56ia	325	5	the	the	DT
cord-300796-rmjv56ia	325	6	asialoglycoprotein	asialoglycoprotein	NNP
cord-300796-rmjv56ia	325	7	receptor	receptor	NN
cord-300796-rmjv56ia	325	8	a	a	DT
cord-300796-rmjv56ia	325	9	site	site	NN
cord-300796-rmjv56ia	325	10	was	be	VBD
cord-300796-rmjv56ia	325	11	not	not	RB
cord-300796-rmjv56ia	325	12	used	use	VBN
cord-300796-rmjv56ia	325	13	if	if	IN
cord-300796-rmjv56ia	325	14	located	locate	VBN
cord-300796-rmjv56ia	325	15	12	12	CD
cord-300796-rmjv56ia	325	16	residues	residue	NNS
cord-300796-rmjv56ia	325	17	apart	apart	RB
cord-300796-rmjv56ia	325	18	from	from	IN
cord-300796-rmjv56ia	325	19	the	the	DT
cord-300796-rmjv56ia	325	20	membrane	membrane	NN
cord-300796-rmjv56ia	325	21	anchor	anchor	NN
cord-300796-rmjv56ia	325	22	,	,	,
cord-300796-rmjv56ia	325	23	however	however	RB
cord-300796-rmjv56ia	325	24	,	,	,
cord-300796-rmjv56ia	325	25	if	if	IN
cord-300796-rmjv56ia	325	26	moved	move	VBN
cord-300796-rmjv56ia	325	27	8	8	CD
cord-300796-rmjv56ia	325	28	more	more	JJR
cord-300796-rmjv56ia	325	29	residues	residue	NNS
cord-300796-rmjv56ia	325	30	apart	apart	RB
cord-300796-rmjv56ia	325	31	from	from	IN
cord-300796-rmjv56ia	325	32	the	the	DT
cord-300796-rmjv56ia	325	33	anchor	anchor	NN
cord-300796-rmjv56ia	325	34	it	-PRON-	PRP
cord-300796-rmjv56ia	325	35	became	become	VBD
cord-300796-rmjv56ia	325	36	glycosylated	glycosylated	JJ
cord-300796-rmjv56ia	325	37	.	.	.
cord-300796-rmjv56ia	326	1	In	in	IN
cord-300796-rmjv56ia	326	2	the	the	DT
cord-300796-rmjv56ia	326	3	case	case	NN
cord-300796-rmjv56ia	326	4	of	of	IN
cord-300796-rmjv56ia	326	5	the	the	DT
cord-300796-rmjv56ia	326	6	Corona	Corona	NNP
cord-300796-rmjv56ia	326	7	virus	virus	NN
cord-300796-rmjv56ia	326	8	protein	protein	NN
cord-300796-rmjv56ia	326	9	a	a	DT
cord-300796-rmjv56ia	326	10	site	site	NN
cord-300796-rmjv56ia	326	11	just	just	RB
cord-300796-rmjv56ia	326	12	adjacent	adjacent	JJ
cord-300796-rmjv56ia	326	13	to	to	IN
cord-300796-rmjv56ia	326	14	the	the	DT
cord-300796-rmjv56ia	326	15	combined	combine	VBN
cord-300796-rmjv56ia	326	16	signal	signal	NN
cord-300796-rmjv56ia	326	17	sequence	sequence	NN
cord-300796-rmjv56ia	326	18	-	-	HYPH
cord-300796-rmjv56ia	326	19	anchor	anchor	NN
cord-300796-rmjv56ia	326	20	peptide	peptide	NN
cord-300796-rmjv56ia	326	21	remained	remain	VBD
cord-300796-rmjv56ia	326	22	unglycosylated	unglycosylated	JJ
cord-300796-rmjv56ia	326	23	,	,	,
cord-300796-rmjv56ia	326	24	whereas	whereas	IN
cord-300796-rmjv56ia	326	25	an	an	DT
cord-300796-rmjv56ia	326	26	engineered	engineer	VBN
cord-300796-rmjv56ia	326	27	site	site	NN
cord-300796-rmjv56ia	326	28	24	24	CD
cord-300796-rmjv56ia	326	29	residues	residue	NNS
cord-300796-rmjv56ia	326	30	further	far	RBR
cord-300796-rmjv56ia	326	31	away	away	RB
cord-300796-rmjv56ia	326	32	was	be	VBD
cord-300796-rmjv56ia	326	33	used	use	VBN
cord-300796-rmjv56ia	326	34	for	for	IN
cord-300796-rmjv56ia	326	35	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	326	36	.	.	.
cord-300796-rmjv56ia	327	1	Such	such	JJ
cord-300796-rmjv56ia	327	2	restrictions	restriction	NNS
cord-300796-rmjv56ia	327	3	in	in	IN
cord-300796-rmjv56ia	327	4	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	327	5	are	be	VBP
cord-300796-rmjv56ia	327	6	most	most	RBS
cord-300796-rmjv56ia	327	7	likely	likely	JJ
cord-300796-rmjv56ia	327	8	to	to	TO
cord-300796-rmjv56ia	327	9	be	be	VB
cord-300796-rmjv56ia	327	10	explained	explain	VBN
cord-300796-rmjv56ia	327	11	by	by	IN
cord-300796-rmjv56ia	327	12	sterical	sterical	JJ
cord-300796-rmjv56ia	327	13	problems	problem	NNS
cord-300796-rmjv56ia	327	14	in	in	IN
cord-300796-rmjv56ia	327	15	attaching	attach	VBG
cord-300796-rmjv56ia	327	16	the	the	DT
cord-300796-rmjv56ia	327	17	very	very	RB
cord-300796-rmjv56ia	327	18	spacious	spacious	JJ
cord-300796-rmjv56ia	327	19	sugar	sugar	NN
cord-300796-rmjv56ia	327	20	unit	unit	NN
cord-300796-rmjv56ia	327	21	(	(	-LRB-
cord-300796-rmjv56ia	327	22	Lee	Lee	NNP
cord-300796-rmjv56ia	327	23	et	et	NNP
cord-300796-rmjv56ia	327	24	al	al	NNP
cord-300796-rmjv56ia	327	25	.	.	NNP
cord-300796-rmjv56ia	327	26	,	,	,
cord-300796-rmjv56ia	327	27	1984	1984	CD
cord-300796-rmjv56ia	327	28	;	;	:
cord-300796-rmjv56ia	327	29	see	see	VB
cord-300796-rmjv56ia	327	30	also	also	RB
cord-300796-rmjv56ia	327	31	Wier	Wier	NNP
cord-300796-rmjv56ia	327	32	and	and	CC
cord-300796-rmjv56ia	327	33	Edidin	Edidin	NNP
cord-300796-rmjv56ia	327	34	,	,	,
cord-300796-rmjv56ia	327	35	1988	1988	CD
cord-300796-rmjv56ia	327	36	)	)	-RRB-
cord-300796-rmjv56ia	327	37	onto	onto	IN
cord-300796-rmjv56ia	327	38	acceptor	acceptor	NN
cord-300796-rmjv56ia	327	39	sites	site	NNS
cord-300796-rmjv56ia	327	40	that	that	WDT
cord-300796-rmjv56ia	327	41	are	be	VBP
cord-300796-rmjv56ia	327	42	fixed	fix	VBN
cord-300796-rmjv56ia	327	43	in	in	IN
cord-300796-rmjv56ia	327	44	a	a	DT
cord-300796-rmjv56ia	327	45	position	position	NN
cord-300796-rmjv56ia	327	46	which	which	WDT
cord-300796-rmjv56ia	327	47	is	be	VBZ
cord-300796-rmjv56ia	327	48	close	close	JJ
cord-300796-rmjv56ia	327	49	to	to	IN
cord-300796-rmjv56ia	327	50	the	the	DT
cord-300796-rmjv56ia	327	51	membrane	membrane	NN
cord-300796-rmjv56ia	327	52	plane	plane	NN
cord-300796-rmjv56ia	327	53	.	.	.
cord-300796-rmjv56ia	328	1	Therefore	therefore	RB
cord-300796-rmjv56ia	328	2	,	,	,
cord-300796-rmjv56ia	328	3	we	-PRON-	PRP
cord-300796-rmjv56ia	328	4	assume	assume	VBP
cord-300796-rmjv56ia	328	5	that	that	IN
cord-300796-rmjv56ia	328	6	the	the	DT
cord-300796-rmjv56ia	328	7	p62	p62	NN
cord-300796-rmjv56ia	328	8	signal	signal	NN
cord-300796-rmjv56ia	328	9	sequence	sequence	NN
cord-300796-rmjv56ia	328	10	,	,	,
cord-300796-rmjv56ia	328	11	with	with	IN
cord-300796-rmjv56ia	328	12	its	-PRON-	PRP$
cord-300796-rmjv56ia	328	13	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	328	14	site	site	NN
cord-300796-rmjv56ia	328	15	at	at	IN
cord-300796-rmjv56ia	328	16	Asn13	Asn13	NNP
cord-300796-rmjv56ia	328	17	,	,	,
cord-300796-rmjv56ia	328	18	can	can	MD
cord-300796-rmjv56ia	328	19	not	not	RB
cord-300796-rmjv56ia	328	20	become	become	VB
cord-300796-rmjv56ia	328	21	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	328	22	before	before	IN
cord-300796-rmjv56ia	328	23	it	-PRON-	PRP
cord-300796-rmjv56ia	328	24	has	have	VBZ
cord-300796-rmjv56ia	328	25	been	be	VBN
cord-300796-rmjv56ia	328	26	released	release	VBN
cord-300796-rmjv56ia	328	27	into	into	IN
cord-300796-rmjv56ia	328	28	ER	ER	NNP
cord-300796-rmjv56ia	328	29	lumen	lumen	NN
cord-300796-rmjv56ia	328	30	.	.	.
cord-300796-rmjv56ia	329	1	As	as	IN
cord-300796-rmjv56ia	329	2	this	this	DT
cord-300796-rmjv56ia	329	3	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	329	4	event	event	NN
cord-300796-rmjv56ia	329	5	was	be	VBD
cord-300796-rmjv56ia	329	6	shown	show	VBN
cord-300796-rmjv56ia	329	7	to	to	TO
cord-300796-rmjv56ia	329	8	occur	occur	VB
cord-300796-rmjv56ia	329	9	at	at	IN
cord-300796-rmjv56ia	329	10	an	an	DT
cord-300796-rmjv56ia	329	11	early	early	JJ
cord-300796-rmjv56ia	329	12	stage	stage	NN
cord-300796-rmjv56ia	329	13	of	of	IN
cord-300796-rmjv56ia	329	14	chain	chain	NN
cord-300796-rmjv56ia	329	15	translocation	translocation	NN
cord-300796-rmjv56ia	329	16	it	-PRON-	PRP
cord-300796-rmjv56ia	329	17	follows	follow	VBZ
cord-300796-rmjv56ia	329	18	that	that	IN
cord-300796-rmjv56ia	329	19	this	this	DT
cord-300796-rmjv56ia	329	20	signal	signal	NN
cord-300796-rmjv56ia	329	21	sequence	sequence	NN
cord-300796-rmjv56ia	329	22	can	can	MD
cord-300796-rmjv56ia	329	23	only	only	RB
cord-300796-rmjv56ia	329	24	interact	interact	VB
cord-300796-rmjv56ia	329	25	with	with	IN
cord-300796-rmjv56ia	329	26	the	the	DT
cord-300796-rmjv56ia	329	27	ER	ER	NNP
cord-300796-rmjv56ia	329	28	membrane	membrane	NN
cord-300796-rmjv56ia	329	29	during	during	IN
cord-300796-rmjv56ia	329	30	the	the	DT
cord-300796-rmjv56ia	329	31	beginning	beginning	NN
cord-300796-rmjv56ia	329	32	of	of	IN
cord-300796-rmjv56ia	329	33	chain	chain	NN
cord-300796-rmjv56ia	329	34	translocation	translocation	NN
cord-300796-rmjv56ia	329	35	.	.	.
cord-300796-rmjv56ia	330	1	In	in	IN
cord-300796-rmjv56ia	330	2	other	other	JJ
cord-300796-rmjv56ia	330	3	words	word	NNS
cord-300796-rmjv56ia	330	4	,	,	,
cord-300796-rmjv56ia	330	5	the	the	DT
cord-300796-rmjv56ia	330	6	signal	signal	JJ
cord-300796-rmjv56ia	330	7	sequence	sequence	NN
cord-300796-rmjv56ia	330	8	of	of	IN
cord-300796-rmjv56ia	330	9	p62	p62	NN
cord-300796-rmjv56ia	330	10	can	can	MD
cord-300796-rmjv56ia	330	11	only	only	RB
cord-300796-rmjv56ia	330	12	function	function	VB
cord-300796-rmjv56ia	330	13	at	at	IN
cord-300796-rmjv56ia	330	14	the	the	DT
cord-300796-rmjv56ia	330	15	initiation	initiation	NN
cord-300796-rmjv56ia	330	16	stage	stage	NN
cord-300796-rmjv56ia	330	17	of	of	IN
cord-300796-rmjv56ia	330	18	chain	chain	NN
cord-300796-rmjv56ia	330	19	translocation	translocation	NN
cord-300796-rmjv56ia	330	20	and	and	CC
cord-300796-rmjv56ia	330	21	has	have	VBZ
cord-300796-rmjv56ia	330	22	no	no	DT
cord-300796-rmjv56ia	330	23	role	role	NN
cord-300796-rmjv56ia	330	24	in	in	IN
cord-300796-rmjv56ia	330	25	completing	complete	VBG
cord-300796-rmjv56ia	330	26	this	this	DT
cord-300796-rmjv56ia	330	27	transfer	transfer	NN
cord-300796-rmjv56ia	330	28	process	process	NN
cord-300796-rmjv56ia	330	29	.	.	.
cord-300796-rmjv56ia	331	1	If	if	IN
cord-300796-rmjv56ia	331	2	the	the	DT
cord-300796-rmjv56ia	331	3	latter	latter	JJ
cord-300796-rmjv56ia	331	4	would	would	MD
cord-300796-rmjv56ia	331	5	be	be	VB
cord-300796-rmjv56ia	331	6	true	true	JJ
cord-300796-rmjv56ia	331	7	we	-PRON-	PRP
cord-300796-rmjv56ia	331	8	would	would	MD
cord-300796-rmjv56ia	331	9	have	have	VB
cord-300796-rmjv56ia	331	10	expected	expect	VBN
cord-300796-rmjv56ia	331	11	that	that	IN
cord-300796-rmjv56ia	331	12	the	the	DT
cord-300796-rmjv56ia	331	13	signal	signal	NN
cord-300796-rmjv56ia	331	14	sequence	sequence	NN
cord-300796-rmjv56ia	331	15	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	331	16	would	would	MD
cord-300796-rmjv56ia	331	17	have	have	VB
cord-300796-rmjv56ia	331	18	occurred	occur	VBN
cord-300796-rmjv56ia	331	19	first	first	RB
cord-300796-rmjv56ia	331	20	after	after	RB
cord-300796-rmjv56ia	331	21	all	all	DT
cord-300796-rmjv56ia	331	22	of	of	IN
cord-300796-rmjv56ia	331	23	the	the	DT
cord-300796-rmjv56ia	331	24	lumenal	lumenal	JJ
cord-300796-rmjv56ia	331	25	domain	domain	NN
cord-300796-rmjv56ia	331	26	of	of	IN
cord-300796-rmjv56ia	331	27	the	the	DT
cord-300796-rmjv56ia	331	28	p62	p62	NN
cord-300796-rmjv56ia	331	29	d-4	d-4	NN
cord-300796-rmjv56ia	331	30	chain	chain	NN
cord-300796-rmjv56ia	331	31	would	would	MD
cord-300796-rmjv56ia	331	32	have	have	VB
cord-300796-rmjv56ia	331	33	been	be	VBN
cord-300796-rmjv56ia	331	34	translated	translate	VBN
cord-300796-rmjv56ia	331	35	and	and	CC
cord-300796-rmjv56ia	331	36	translocated	translocate	VBN
cord-300796-rmjv56ia	331	37	.	.	.
cord-300796-rmjv56ia	332	1	The	the	DT
cord-300796-rmjv56ia	332	2	importance	importance	NN
cord-300796-rmjv56ia	332	3	of	of	IN
cord-300796-rmjv56ia	332	4	our	-PRON-	PRP$
cord-300796-rmjv56ia	332	5	results	result	NNS
cord-300796-rmjv56ia	332	6	in	in	IN
cord-300796-rmjv56ia	332	7	this	this	DT
cord-300796-rmjv56ia	332	8	work	work	NN
cord-300796-rmjv56ia	332	9	lies	lie	VBZ
cord-300796-rmjv56ia	332	10	in	in	IN
cord-300796-rmjv56ia	332	11	the	the	DT
cord-300796-rmjv56ia	332	12	fact	fact	NN
cord-300796-rmjv56ia	332	13	that	that	IN
cord-300796-rmjv56ia	332	14	they	-PRON-	PRP
cord-300796-rmjv56ia	332	15	rule	rule	VBP
cord-300796-rmjv56ia	332	16	out	out	RP
cord-300796-rmjv56ia	332	17	translocation	translocation	NN
cord-300796-rmjv56ia	332	18	models	model	NNS
cord-300796-rmjv56ia	332	19	in	in	IN
cord-300796-rmjv56ia	332	20	which	which	WDT
cord-300796-rmjv56ia	332	21	the	the	DT
cord-300796-rmjv56ia	332	22	signal	signal	NN
cord-300796-rmjv56ia	332	23	sequence	sequence	NN
cord-300796-rmjv56ia	332	24	would	would	MD
cord-300796-rmjv56ia	332	25	have	have	VB
cord-300796-rmjv56ia	332	26	a	a	DT
cord-300796-rmjv56ia	332	27	role	role	NN
cord-300796-rmjv56ia	332	28	throughout	throughout	IN
cord-300796-rmjv56ia	332	29	the	the	DT
cord-300796-rmjv56ia	332	30	whole	whole	JJ
cord-300796-rmjv56ia	332	31	process	process	NN
cord-300796-rmjv56ia	332	32	of	of	IN
cord-300796-rmjv56ia	332	33	chain	chain	NN
cord-300796-rmjv56ia	332	34	translocation	translocation	NN
cord-300796-rmjv56ia	332	35	.	.	.
cord-300796-rmjv56ia	333	1	For	for	IN
cord-300796-rmjv56ia	333	2	instance	instance	NN
cord-300796-rmjv56ia	333	3	,	,	,
cord-300796-rmjv56ia	333	4	if	if	IN
cord-300796-rmjv56ia	333	5	the	the	DT
cord-300796-rmjv56ia	333	6	translocation	translocation	NN
cord-300796-rmjv56ia	333	7	site	site	NN
cord-300796-rmjv56ia	333	8	is	be	VBZ
cord-300796-rmjv56ia	333	9	represented	represent	VBN
cord-300796-rmjv56ia	333	10	by	by	IN
cord-300796-rmjv56ia	333	11	a	a	DT
cord-300796-rmjv56ia	333	12	multisubunit	multisubunit	NN
cord-300796-rmjv56ia	333	13	protein	protein	NN
cord-300796-rmjv56ia	333	14	complex	complex	NN
cord-300796-rmjv56ia	333	15	forming	form	VBG
cord-300796-rmjv56ia	333	16	an	an	DT
cord-300796-rmjv56ia	333	17	aqueous	aqueous	JJ
cord-300796-rmjv56ia	333	18	channel	channel	NN
cord-300796-rmjv56ia	333	19	across	across	IN
cord-300796-rmjv56ia	333	20	the	the	DT
cord-300796-rmjv56ia	333	21	membrane	membrane	NN
cord-300796-rmjv56ia	333	22	for	for	IN
cord-300796-rmjv56ia	333	23	chain	chain	NN
cord-300796-rmjv56ia	333	24	transfer	transfer	NN
cord-300796-rmjv56ia	333	25	(	(	-LRB-
cord-300796-rmjv56ia	333	26	see	see	VB
cord-300796-rmjv56ia	333	27	signal	signal	NN
cord-300796-rmjv56ia	333	28	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	333	29	,	,	,
cord-300796-rmjv56ia	333	30	Blobel	Blobel	NNP
cord-300796-rmjv56ia	333	31	and	and	CC
cord-300796-rmjv56ia	333	32	Dobberstein	Dobberstein	NNP
cord-300796-rmjv56ia	333	33	,	,	,
cord-300796-rmjv56ia	333	34	1975	1975	CD
cord-300796-rmjv56ia	333	35	;	;	:
cord-300796-rmjv56ia	333	36	amphiphatic	amphiphatic	NNP
cord-300796-rmjv56ia	333	37	tunnel	tunnel	NN
cord-300796-rmjv56ia	333	38	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	333	39	,	,	,
cord-300796-rmjv56ia	333	40	Rapoport	Rapoport	NNP
cord-300796-rmjv56ia	333	41	,	,	,
cord-300796-rmjv56ia	333	42	1985	1985	CD
cord-300796-rmjv56ia	333	43	;	;	:
cord-300796-rmjv56ia	334	1	translocator	translocator	NN
cord-300796-rmjv56ia	334	2	protein	protein	NN
cord-300796-rmjv56ia	334	3	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	334	4	,	,	,
cord-300796-rmjv56ia	334	5	Singer	Singer	NNP
cord-300796-rmjv56ia	334	6	et	et	NNP
cord-300796-rmjv56ia	334	7	al	al	NNP
cord-300796-rmjv56ia	334	8	.	.	NNP
cord-300796-rmjv56ia	334	9	,	,	,
cord-300796-rmjv56ia	334	10	1987a	1987a	CD
cord-300796-rmjv56ia	334	11	,	,	,
cord-300796-rmjv56ia	334	12	b	b	LS
cord-300796-rmjv56ia	334	13	)	)	-RRB-
cord-300796-rmjv56ia	334	14	,	,	,
cord-300796-rmjv56ia	334	15	then	then	RB
cord-300796-rmjv56ia	334	16	the	the	DT
cord-300796-rmjv56ia	334	17	signal	signal	NN
cord-300796-rmjv56ia	334	18	sequence	sequence	NN
cord-300796-rmjv56ia	334	19	could	could	MD
cord-300796-rmjv56ia	334	20	be	be	VB
cord-300796-rmjv56ia	334	21	involved	involve	VBN
cord-300796-rmjv56ia	334	22	in	in	IN
cord-300796-rmjv56ia	334	23	its	-PRON-	PRP$
cord-300796-rmjv56ia	334	24	assembly	assembly	NN
cord-300796-rmjv56ia	334	25	or	or	CC
cord-300796-rmjv56ia	334	26	"	"	``
cord-300796-rmjv56ia	334	27	opening	opening	NN
cord-300796-rmjv56ia	334	28	"	"	''
cord-300796-rmjv56ia	334	29	but	but	CC
cord-300796-rmjv56ia	334	30	apparently	apparently	RB
cord-300796-rmjv56ia	334	31	not	not	RB
cord-300796-rmjv56ia	334	32	for	for	IN
cord-300796-rmjv56ia	334	33	keeping	keep	VBG
cord-300796-rmjv56ia	334	34	it	-PRON-	PRP
cord-300796-rmjv56ia	334	35	together	together	RB
cord-300796-rmjv56ia	334	36	or	or	CC
cord-300796-rmjv56ia	334	37	open	open	JJ
cord-300796-rmjv56ia	334	38	until	until	IN
cord-300796-rmjv56ia	334	39	chain	chain	NN
cord-300796-rmjv56ia	334	40	transfer	transfer	NN
cord-300796-rmjv56ia	334	41	is	be	VBZ
cord-300796-rmjv56ia	334	42	completed	complete	VBN
cord-300796-rmjv56ia	334	43	(	(	-LRB-
cord-300796-rmjv56ia	334	44	as	as	IN
cord-300796-rmjv56ia	334	45	suggested	suggest	VBN
cord-300796-rmjv56ia	334	46	in	in	IN
cord-300796-rmjv56ia	334	47	Singer	Singer	NNP
cord-300796-rmjv56ia	334	48	et	et	NNP
cord-300796-rmjv56ia	334	49	al	al	NNP
cord-300796-rmjv56ia	334	50	.	.	NNP
cord-300796-rmjv56ia	334	51	,	,	,
cord-300796-rmjv56ia	334	52	1987a	1987a	CD
cord-300796-rmjv56ia	334	53	)	)	-RRB-
cord-300796-rmjv56ia	334	54	.	.	.
cord-300796-rmjv56ia	335	1	Similarly	similarly	RB
cord-300796-rmjv56ia	335	2	,	,	,
cord-300796-rmjv56ia	335	3	when	when	WRB
cord-300796-rmjv56ia	335	4	considering	consider	VBG
cord-300796-rmjv56ia	335	5	models	model	NNS
cord-300796-rmjv56ia	335	6	in	in	IN
cord-300796-rmjv56ia	335	7	which	which	WDT
cord-300796-rmjv56ia	335	8	the	the	DT
cord-300796-rmjv56ia	335	9	chain	chain	NN
cord-300796-rmjv56ia	335	10	transfer	transfer	NN
cord-300796-rmjv56ia	335	11	occurs	occur	VBZ
cord-300796-rmjv56ia	335	12	directly	directly	RB
cord-300796-rmjv56ia	335	13	through	through	IN
cord-300796-rmjv56ia	335	14	a	a	DT
cord-300796-rmjv56ia	335	15	lipid	lipid	NN
cord-300796-rmjv56ia	335	16	membrane	membrane	NN
cord-300796-rmjv56ia	335	17	(	(	-LRB-
cord-300796-rmjv56ia	335	18	see	see	VB
cord-300796-rmjv56ia	335	19	the	the	DT
cord-300796-rmjv56ia	335	20	helical	helical	JJ
cord-300796-rmjv56ia	335	21	hairpin	hairpin	NN
cord-300796-rmjv56ia	335	22	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	335	23	,	,	,
cord-300796-rmjv56ia	335	24	Engelrnan	Engelrnan	NNP
cord-300796-rmjv56ia	335	25	and	and	CC
cord-300796-rmjv56ia	335	26	Steitz	Steitz	NNP
cord-300796-rmjv56ia	335	27	,	,	,
cord-300796-rmjv56ia	335	28	1981	1981	CD
cord-300796-rmjv56ia	335	29	;	;	:
cord-300796-rmjv56ia	335	30	direct	direct	JJ
cord-300796-rmjv56ia	335	31	transfer	transfer	NN
cord-300796-rmjv56ia	335	32	model	model	NN
cord-300796-rmjv56ia	335	33	,	,	,
cord-300796-rmjv56ia	335	34	von	von	NNP
cord-300796-rmjv56ia	335	35	Heijne	Heijne	NNP
cord-300796-rmjv56ia	335	36	and	and	CC
cord-300796-rmjv56ia	335	37	Blomberg	Blomberg	NNP
cord-300796-rmjv56ia	335	38	,	,	,
cord-300796-rmjv56ia	335	39	1979	1979	CD
cord-300796-rmjv56ia	335	40	;	;	:
cord-300796-rmjv56ia	335	41	phospholipid	phospholipid	NN
cord-300796-rmjv56ia	335	42	channel	channel	NN
cord-300796-rmjv56ia	335	43	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	335	44	,	,	,
cord-300796-rmjv56ia	335	45	Nesmayanova	Nesmayanova	NNP
cord-300796-rmjv56ia	335	46	,	,	,
cord-300796-rmjv56ia	335	47	1982	1982	CD
cord-300796-rmjv56ia	335	48	)	)	-RRB-
cord-300796-rmjv56ia	336	1	the	the	DT
cord-300796-rmjv56ia	336	2	interaction	interaction	NN
cord-300796-rmjv56ia	336	3	of	of	IN
cord-300796-rmjv56ia	336	4	the	the	DT
cord-300796-rmjv56ia	336	5	signal	signal	JJ
cord-300796-rmjv56ia	336	6	sequence	sequence	NN
cord-300796-rmjv56ia	336	7	with	with	IN
cord-300796-rmjv56ia	336	8	the	the	DT
cord-300796-rmjv56ia	336	9	lipid	lipid	NN
cord-300796-rmjv56ia	336	10	bilayer	bilayer	NN
cord-300796-rmjv56ia	336	11	could	could	MD
cord-300796-rmjv56ia	336	12	be	be	VB
cord-300796-rmjv56ia	336	13	of	of	IN
cord-300796-rmjv56ia	336	14	importance	importance	NN
cord-300796-rmjv56ia	336	15	only	only	RB
cord-300796-rmjv56ia	336	16	at	at	IN
cord-300796-rmjv56ia	336	17	the	the	DT
cord-300796-rmjv56ia	336	18	stage	stage	NN
cord-300796-rmjv56ia	336	19	of	of	IN
cord-300796-rmjv56ia	336	20	translocation	translocation	NN
cord-300796-rmjv56ia	336	21	initiation	initiation	NN
cord-300796-rmjv56ia	336	22	but	but	CC
cord-300796-rmjv56ia	336	23	not	not	RB
cord-300796-rmjv56ia	336	24	at	at	IN
cord-300796-rmjv56ia	336	25	the	the	DT
cord-300796-rmjv56ia	336	26	actual	actual	JJ
cord-300796-rmjv56ia	336	27	chain	chain	NN
cord-300796-rmjv56ia	336	28	transfer	transfer	NN
cord-300796-rmjv56ia	336	29	step	step	NN
cord-300796-rmjv56ia	336	30	.	.	.
cord-300796-rmjv56ia	337	1	The	the	DT
cord-300796-rmjv56ia	337	2	possibility	possibility	NN
cord-300796-rmjv56ia	337	3	that	that	IN
cord-300796-rmjv56ia	337	4	our	-PRON-	PRP$
cord-300796-rmjv56ia	337	5	results	result	NNS
cord-300796-rmjv56ia	337	6	about	about	IN
cord-300796-rmjv56ia	337	7	p62	p62	NN
cord-300796-rmjv56ia	337	8	protein	protein	NN
cord-300796-rmjv56ia	337	9	translocation	translocation	NN
cord-300796-rmjv56ia	337	10	would	would	MD
cord-300796-rmjv56ia	337	11	be	be	VB
cord-300796-rmjv56ia	337	12	unique	unique	JJ
cord-300796-rmjv56ia	337	13	to	to	IN
cord-300796-rmjv56ia	337	14	the	the	DT
cord-300796-rmjv56ia	337	15	viral	viral	JJ
cord-300796-rmjv56ia	337	16	system	system	NN
cord-300796-rmjv56ia	337	17	.and	.and	.
cord-300796-rmjv56ia	338	1	different	different	JJ
cord-300796-rmjv56ia	338	2	from	from	IN
cord-300796-rmjv56ia	338	3	the	the	DT
cord-300796-rmjv56ia	338	4	general	general	JJ
cord-300796-rmjv56ia	338	5	translocation	translocation	NN
cord-300796-rmjv56ia	338	6	process	process	NN
cord-300796-rmjv56ia	338	7	in	in	IN
cord-300796-rmjv56ia	338	8	the	the	DT
cord-300796-rmjv56ia	338	9	ER	er	NN
cord-300796-rmjv56ia	338	10	we	-PRON-	PRP
cord-300796-rmjv56ia	338	11	find	find	VBP
cord-300796-rmjv56ia	338	12	most	most	RBS
cord-300796-rmjv56ia	338	13	unlikely	unlikely	JJ
cord-300796-rmjv56ia	338	14	.	.	.
cord-300796-rmjv56ia	339	1	Several	several	JJ
cord-300796-rmjv56ia	339	2	results	result	NNS
cord-300796-rmjv56ia	339	3	from	from	IN
cord-300796-rmjv56ia	339	4	this	this	DT
cord-300796-rmjv56ia	339	5	and	and	CC
cord-300796-rmjv56ia	339	6	earlier	early	JJR
cord-300796-rmjv56ia	339	7	works	work	NNS
cord-300796-rmjv56ia	339	8	suggest	suggest	VBP
cord-300796-rmjv56ia	339	9	that	that	IN
cord-300796-rmjv56ia	339	10	the	the	DT
cord-300796-rmjv56ia	339	11	signal	signal	JJ
cord-300796-rmjv56ia	339	12	sequence	sequence	NN
cord-300796-rmjv56ia	339	13	of	of	IN
cord-300796-rmjv56ia	339	14	the	the	DT
cord-300796-rmjv56ia	339	15	p62	p62	NN
cord-300796-rmjv56ia	339	16	protein	protein	NN
cord-300796-rmjv56ia	339	17	functions	function	NNS
cord-300796-rmjv56ia	339	18	much	much	JJ
cord-300796-rmjv56ia	339	19	in	in	IN
cord-300796-rmjv56ia	339	20	the	the	DT
cord-300796-rmjv56ia	339	21	same	same	JJ
cord-300796-rmjv56ia	339	22	way	way	NN
cord-300796-rmjv56ia	339	23	as	as	IN
cord-300796-rmjv56ia	339	24	cleavable	cleavable	JJ
cord-300796-rmjv56ia	339	25	ones	one	NNS
cord-300796-rmjv56ia	339	26	do	do	VBP
cord-300796-rmjv56ia	339	27	.	.	.
cord-300796-rmjv56ia	340	1	Firstly	firstly	RB
cord-300796-rmjv56ia	340	2	,	,	,
cord-300796-rmjv56ia	340	3	studies	study	NNS
cord-300796-rmjv56ia	340	4	with	with	IN
cord-300796-rmjv56ia	340	5	a	a	DT
cord-300796-rmjv56ia	340	6	temperature	temperature	NN
cord-300796-rmjv56ia	340	7	-	-	HYPH
cord-300796-rmjv56ia	340	8	sensitive	sensitive	JJ
cord-300796-rmjv56ia	340	9	mutant	mutant	NN
cord-300796-rmjv56ia	340	10	of	of	IN
cord-300796-rmjv56ia	340	11	SFV	SFV	NNP
cord-300796-rmjv56ia	340	12	,	,	,
cord-300796-rmjv56ia	340	13	ts3	ts3	NN
cord-300796-rmjv56ia	340	14	,	,	,
cord-300796-rmjv56ia	340	15	have	have	VBP
cord-300796-rmjv56ia	340	16	shown	show	VBN
cord-300796-rmjv56ia	340	17	that	that	IN
cord-300796-rmjv56ia	340	18	the	the	DT
cord-300796-rmjv56ia	340	19	signal	signal	JJ
cord-300796-rmjv56ia	340	20	sequence	sequence	NN
cord-300796-rmjv56ia	340	21	of	of	IN
cord-300796-rmjv56ia	340	22	p62	p62	NN
cord-300796-rmjv56ia	340	23	requires	require	VBZ
cord-300796-rmjv56ia	340	24	a	a	DT
cord-300796-rmjv56ia	340	25	free	free	JJ
cord-300796-rmjv56ia	340	26	NH2-terminal	nh2-terminal	JJ
cord-300796-rmjv56ia	340	27	end	end	NN
cord-300796-rmjv56ia	340	28	for	for	IN
cord-300796-rmjv56ia	340	29	function	function	NN
cord-300796-rmjv56ia	341	1	(	(	-LRB-
cord-300796-rmjv56ia	341	2	Hashimoto	Hashimoto	NNP
cord-300796-rmjv56ia	341	3	et	et	NNP
cord-300796-rmjv56ia	341	4	al	al	NNP
cord-300796-rmjv56ia	341	5	.	.	.
cord-300796-rmjv56ia	342	1	,	,	,
cord-300796-rmjv56ia	342	2	1981	1981	CD
cord-300796-rmjv56ia	342	3	)	)	-RRB-
cord-300796-rmjv56ia	342	4	.	.	.
cord-300796-rmjv56ia	343	1	At	at	IN
cord-300796-rmjv56ia	343	2	the	the	DT
cord-300796-rmjv56ia	343	3	nonpermissive	nonpermissive	JJ
cord-300796-rmjv56ia	343	4	temperature	temperature	NN
cord-300796-rmjv56ia	343	5	the	the	DT
cord-300796-rmjv56ia	343	6	ts3	ts3	NN
cord-300796-rmjv56ia	343	7	mutant	mutant	NN
cord-300796-rmjv56ia	343	8	is	be	VBZ
cord-300796-rmjv56ia	343	9	defective	defective	JJ
cord-300796-rmjv56ia	343	10	in	in	IN
cord-300796-rmjv56ia	343	11	cleavage	cleavage	NN
cord-300796-rmjv56ia	343	12	between	between	IN
cord-300796-rmjv56ia	343	13	the	the	DT
cord-300796-rmjv56ia	343	14	C	C	NNP
cord-300796-rmjv56ia	343	15	and	and	CC
cord-300796-rmjv56ia	343	16	the	the	DT
cord-300796-rmjv56ia	343	17	p62	p62	NN
cord-300796-rmjv56ia	343	18	protein	protein	NN
cord-300796-rmjv56ia	343	19	region	region	NN
cord-300796-rmjv56ia	343	20	of	of	IN
cord-300796-rmjv56ia	343	21	the	the	DT
cord-300796-rmjv56ia	343	22	protein	protein	NN
cord-300796-rmjv56ia	343	23	precursor	precursor	NN
cord-300796-rmjv56ia	343	24	because	because	IN
cord-300796-rmjv56ia	343	25	of	of	IN
cord-300796-rmjv56ia	343	26	a	a	DT
cord-300796-rmjv56ia	343	27	mutation	mutation	NN
cord-300796-rmjv56ia	343	28	that	that	WDT
cord-300796-rmjv56ia	343	29	inactivates	inactivate	VBZ
cord-300796-rmjv56ia	343	30	the	the	DT
cord-300796-rmjv56ia	343	31	autoproteolytic	autoproteolytic	JJ
cord-300796-rmjv56ia	343	32	activity	activity	NN
cord-300796-rmjv56ia	343	33	of	of	IN
cord-300796-rmjv56ia	343	34	C.	C.	NNP
cord-300796-rmjv56ia	344	1	This	this	DT
cord-300796-rmjv56ia	344	2	defect	defect	NN
cord-300796-rmjv56ia	344	3	results	result	VBZ
cord-300796-rmjv56ia	344	4	in	in	IN
cord-300796-rmjv56ia	344	5	a	a	DT
cord-300796-rmjv56ia	344	6	translocation	translocation	NN
cord-300796-rmjv56ia	344	7	negative	negative	JJ
cord-300796-rmjv56ia	344	8	phenotype	phenotype	NN
cord-300796-rmjv56ia	344	9	for	for	IN
cord-300796-rmjv56ia	344	10	the	the	DT
cord-300796-rmjv56ia	344	11	p62	p62	NN
cord-300796-rmjv56ia	344	12	protein	protein	NN
cord-300796-rmjv56ia	344	13	.	.	.
cord-300796-rmjv56ia	345	1	Secondly	secondly	RB
cord-300796-rmjv56ia	345	2	,	,	,
cord-300796-rmjv56ia	345	3	the	the	DT
cord-300796-rmjv56ia	345	4	p62	p62	NN
cord-300796-rmjv56ia	345	5	signal	signal	NN
cord-300796-rmjv56ia	345	6	sequence	sequence	NN
cord-300796-rmjv56ia	345	7	has	have	VBZ
cord-300796-rmjv56ia	345	8	been	be	VBN
cord-300796-rmjv56ia	345	9	shown	show	VBN
cord-300796-rmjv56ia	345	10	to	to	TO
cord-300796-rmjv56ia	345	11	be	be	VB
cord-300796-rmjv56ia	345	12	SRP	SRP	NNP
cord-300796-rmjv56ia	345	13	dependent	dependent	JJ
cord-300796-rmjv56ia	345	14	.	.	.
cord-300796-rmjv56ia	346	1	If	if	IN
cord-300796-rmjv56ia	346	2	the	the	DT
cord-300796-rmjv56ia	346	3	mRNA	mrna	NN
cord-300796-rmjv56ia	346	4	for	for	IN
cord-300796-rmjv56ia	346	5	the	the	DT
cord-300796-rmjv56ia	346	6	structural	structural	JJ
cord-300796-rmjv56ia	346	7	proteins	protein	NNS
cord-300796-rmjv56ia	346	8	of	of	IN
cord-300796-rmjv56ia	346	9	SFV	SFV	NNP
cord-300796-rmjv56ia	346	10	is	be	VBZ
cord-300796-rmjv56ia	346	11	translated	translate	VBN
cord-300796-rmjv56ia	346	12	in	in	IN
cord-300796-rmjv56ia	346	13	vitro	vitro	FW
cord-300796-rmjv56ia	346	14	in	in	IN
cord-300796-rmjv56ia	346	15	a	a	DT
cord-300796-rmjv56ia	346	16	wheat	wheat	NN
cord-300796-rmjv56ia	346	17	germ	germ	NN
cord-300796-rmjv56ia	346	18	-	-	HYPH
cord-300796-rmjv56ia	346	19	derived	derive	VBN
cord-300796-rmjv56ia	346	20	system	system	NN
cord-300796-rmjv56ia	346	21	that	that	WDT
cord-300796-rmjv56ia	346	22	is	be	VBZ
cord-300796-rmjv56ia	346	23	supplemented	supplement	VBN
cord-300796-rmjv56ia	346	24	with	with	IN
cord-300796-rmjv56ia	346	25	saltwashed	saltwashed	NNP
cord-300796-rmjv56ia	346	26	(	(	-LRB-
cord-300796-rmjv56ia	346	27	and	and	CC
cord-300796-rmjv56ia	346	28	SRP	SRP	NNP
cord-300796-rmjv56ia	346	29	-	-	HYPH
cord-300796-rmjv56ia	346	30	deprived	deprive	VBN
cord-300796-rmjv56ia	346	31	)	)	-RRB-
cord-300796-rmjv56ia	346	32	membranes	membrane	NNS
cord-300796-rmjv56ia	346	33	then	then	RB
cord-300796-rmjv56ia	346	34	p62	p62	NN
cord-300796-rmjv56ia	346	35	translocation	translocation	NN
cord-300796-rmjv56ia	346	36	is	be	VBZ
cord-300796-rmjv56ia	346	37	observed	observe	VBN
cord-300796-rmjv56ia	346	38	only	only	RB
cord-300796-rmjv56ia	346	39	in	in	IN
cord-300796-rmjv56ia	346	40	the	the	DT
cord-300796-rmjv56ia	346	41	presence	presence	NN
cord-300796-rmjv56ia	346	42	of	of	IN
cord-300796-rmjv56ia	346	43	exogenous	exogenous	JJ
cord-300796-rmjv56ia	346	44	SRP	SRP	NNP
cord-300796-rmjv56ia	346	45	(	(	-LRB-
cord-300796-rmjv56ia	346	46	Bonatti	Bonatti	NNP
cord-300796-rmjv56ia	346	47	et	et	NNP
cord-300796-rmjv56ia	346	48	al	al	NNP
cord-300796-rmjv56ia	346	49	.	.	NNP
cord-300796-rmjv56ia	346	50	,	,	,
cord-300796-rmjv56ia	346	51	1984	1984	CD
cord-300796-rmjv56ia	346	52	)	)	-RRB-
cord-300796-rmjv56ia	347	1	~	~	NFP
cord-300796-rmjv56ia	347	2	If	if	IN
cord-300796-rmjv56ia	347	3	SRP	SRP	NNP
cord-300796-rmjv56ia	347	4	is	be	VBZ
cord-300796-rmjv56ia	347	5	supplemented	supplement	VBN
cord-300796-rmjv56ia	347	6	without	without	IN
cord-300796-rmjv56ia	347	7	membranes	membrane	NNS
cord-300796-rmjv56ia	347	8	then	then	RB
cord-300796-rmjv56ia	347	9	p62	p62	NN
cord-300796-rmjv56ia	347	10	translation	translation	NN
cord-300796-rmjv56ia	347	11	is	be	VBZ
cord-300796-rmjv56ia	347	12	arrested	arrest	VBN
cord-300796-rmjv56ia	347	13	.	.	.
cord-300796-rmjv56ia	348	1	Thirdly	thirdly	RB
cord-300796-rmjv56ia	348	2	,	,	,
cord-300796-rmjv56ia	348	3	our	-PRON-	PRP$
cord-300796-rmjv56ia	348	4	time	time	NN
cord-300796-rmjv56ia	348	5	course	course	NN
cord-300796-rmjv56ia	348	6	study	study	NN
cord-300796-rmjv56ia	348	7	about	about	IN
cord-300796-rmjv56ia	348	8	p62	p62	NN
cord-300796-rmjv56ia	348	9	synthesis	synthesis	NN
cord-300796-rmjv56ia	348	10	and	and	CC
cord-300796-rmjv56ia	348	11	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	348	12	in	in	IN
cord-300796-rmjv56ia	348	13	this	this	DT
cord-300796-rmjv56ia	348	14	work	work	NN
cord-300796-rmjv56ia	348	15	clearly	clearly	RB
cord-300796-rmjv56ia	348	16	demonstrates	demonstrate	VBZ
cord-300796-rmjv56ia	348	17	that	that	IN
cord-300796-rmjv56ia	348	18	the	the	DT
cord-300796-rmjv56ia	348	19	p62	p62	NN
cord-300796-rmjv56ia	348	20	chain	chain	NN
cord-300796-rmjv56ia	348	21	is	be	VBZ
cord-300796-rmjv56ia	348	22	translocated	translocate	VBN
cord-300796-rmjv56ia	348	23	cotranslationally	cotranslationally	RB
cord-300796-rmjv56ia	348	24	across	across	IN
cord-300796-rmjv56ia	348	25	the	the	DT
cord-300796-rmjv56ia	348	26	ER	ER	NNP
cord-300796-rmjv56ia	348	27	membrane	membrane	NN
cord-300796-rmjv56ia	348	28	.	.	.
cord-300796-rmjv56ia	349	1	This	this	DT
cord-300796-rmjv56ia	349	2	was	be	VBD
cord-300796-rmjv56ia	349	3	also	also	RB
cord-300796-rmjv56ia	349	4	suggested	suggest	VBN
cord-300796-rmjv56ia	349	5	by	by	IN
cord-300796-rmjv56ia	349	6	earlier	early	JJR
cord-300796-rmjv56ia	349	7	studies	study	NNS
cord-300796-rmjv56ia	349	8	in	in	IN
cord-300796-rmjv56ia	349	9	vitro	vitro	FW
cord-300796-rmjv56ia	349	10	(	(	-LRB-
cord-300796-rmjv56ia	349	11	Garoff	Garoff	NNP
cord-300796-rmjv56ia	349	12	et	et	FW
cord-300796-rmjv56ia	349	13	al	al	NNP
cord-300796-rmjv56ia	349	14	.	.	NNP
cord-300796-rmjv56ia	349	15	,	,	,
cord-300796-rmjv56ia	349	16	1978	1978	CD
cord-300796-rmjv56ia	349	17	;	;	:
cord-300796-rmjv56ia	350	1	Bonatti	Bonatti	NNP
cord-300796-rmjv56ia	350	2	et	et	NNP
cord-300796-rmjv56ia	350	3	al	al	NNP
cord-300796-rmjv56ia	350	4	.	.	NNP
cord-300796-rmjv56ia	350	5	,	,	,
cord-300796-rmjv56ia	350	6	1984	1984	CD
cord-300796-rmjv56ia	350	7	)	)	-RRB-
cord-300796-rmjv56ia	350	8	.	.	.
cord-300796-rmjv56ia	351	1	In	in	IN
cord-300796-rmjv56ia	351	2	these	these	DT
cord-300796-rmjv56ia	351	3	studies	study	NNS
cord-300796-rmjv56ia	351	4	it	-PRON-	PRP
cord-300796-rmjv56ia	351	5	was	be	VBD
cord-300796-rmjv56ia	351	6	shown	show	VBN
cord-300796-rmjv56ia	351	7	that	that	IN
cord-300796-rmjv56ia	351	8	both	both	DT
cord-300796-rmjv56ia	351	9	microsomal	microsomal	NNP
cord-300796-rmjv56ia	351	10	membranes	membrane	NNS
cord-300796-rmjv56ia	351	11	as	as	RB
cord-300796-rmjv56ia	351	12	well	well	RB
cord-300796-rmjv56ia	351	13	as	as	IN
cord-300796-rmjv56ia	351	14	SRP	SRP	NNP
cord-300796-rmjv56ia	351	15	have	have	VBP
cord-300796-rmjv56ia	351	16	to	to	TO
cord-300796-rmjv56ia	351	17	be	be	VB
cord-300796-rmjv56ia	351	18	added	add	VBN
cord-300796-rmjv56ia	351	19	to	to	IN
cord-300796-rmjv56ia	351	20	the	the	DT
cord-300796-rmjv56ia	351	21	synthesis	synthesis	NN
cord-300796-rmjv56ia	351	22	mixture	mixture	NN
cord-300796-rmjv56ia	351	23	before	before	IN
cord-300796-rmjv56ia	351	24	extensive	extensive	JJ
cord-300796-rmjv56ia	351	25	lengths	length	NNS
cord-300796-rmjv56ia	351	26	(	(	-LRB-
cord-300796-rmjv56ia	351	27	,	,	,
cord-300796-rmjv56ia	351	28	,	,	,
cord-300796-rmjv56ia	351	29	o100	o100	NN
cord-300796-rmjv56ia	351	30	amino	amino	NN
cord-300796-rmjv56ia	351	31	acid	acid	NN
cord-300796-rmjv56ia	351	32	residues	residue	NNS
cord-300796-rmjv56ia	351	33	)	)	-RRB-
cord-300796-rmjv56ia	351	34	of	of	IN
cord-300796-rmjv56ia	351	35	the	the	DT
cord-300796-rmjv56ia	351	36	p62	p62	NN
cord-300796-rmjv56ia	351	37	chains	chain	NNS
cord-300796-rmjv56ia	351	38	have	have	VBP
cord-300796-rmjv56ia	351	39	been	be	VBN
cord-300796-rmjv56ia	351	40	translated	translate	VBN
cord-300796-rmjv56ia	351	41	.	.	.
cord-300796-rmjv56ia	352	1	It	-PRON-	PRP
cord-300796-rmjv56ia	352	2	is	be	VBZ
cord-300796-rmjv56ia	352	3	also	also	RB
cord-300796-rmjv56ia	352	4	possible	possible	JJ
cord-300796-rmjv56ia	352	5	to	to	TO
cord-300796-rmjv56ia	352	6	speculate	speculate	VB
cord-300796-rmjv56ia	352	7	on	on	IN
cord-300796-rmjv56ia	352	8	a	a	DT
cord-300796-rmjv56ia	352	9	mechanism	mechanism	NN
cord-300796-rmjv56ia	352	10	in	in	IN
cord-300796-rmjv56ia	352	11	which	which	WDT
cord-300796-rmjv56ia	352	12	the	the	DT
cord-300796-rmjv56ia	352	13	p62	p62	NN
cord-300796-rmjv56ia	352	14	signal	signal	NN
cord-300796-rmjv56ia	352	15	sequence	sequence	NN
cord-300796-rmjv56ia	352	16	would	would	MD
cord-300796-rmjv56ia	352	17	be	be	VB
cord-300796-rmjv56ia	352	18	released	release	VBN
cord-300796-rmjv56ia	352	19	from	from	IN
cord-300796-rmjv56ia	352	20	a	a	DT
cord-300796-rmjv56ia	352	21	putative	putative	JJ
cord-300796-rmjv56ia	352	22	translocation	translocation	NN
cord-300796-rmjv56ia	352	23	site	site	NN
cord-300796-rmjv56ia	352	24	by	by	IN
cord-300796-rmjv56ia	352	25	being	be	VBG
cord-300796-rmjv56ia	352	26	replaced	replace	VBN
cord-300796-rmjv56ia	352	27	by	by	IN
cord-300796-rmjv56ia	352	28	another	another	DT
cord-300796-rmjv56ia	352	29	signal	signal	JJ
cord-300796-rmjv56ia	352	30	sequence	sequence	NN
cord-300796-rmjv56ia	352	31	-	-	HYPH
cord-300796-rmjv56ia	352	32	like	like	JJ
cord-300796-rmjv56ia	352	33	structure	structure	NN
cord-300796-rmjv56ia	352	34	in	in	IN
cord-300796-rmjv56ia	352	35	the	the	DT
cord-300796-rmjv56ia	352	36	p62	p62	NN
cord-300796-rmjv56ia	352	37	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	352	38	.	.	.
cord-300796-rmjv56ia	353	1	However	however	RB
cord-300796-rmjv56ia	353	2	,	,	,
cord-300796-rmjv56ia	353	3	such	such	PDT
cord-300796-rmjv56ia	353	4	a	a	DT
cord-300796-rmjv56ia	353	5	"	"	``
cord-300796-rmjv56ia	353	6	rescue	rescue	NN
cord-300796-rmjv56ia	353	7	"	"	''
cord-300796-rmjv56ia	353	8	mechanism	mechanism	NN
cord-300796-rmjv56ia	353	9	appears	appear	VBZ
cord-300796-rmjv56ia	353	10	improbable	improbable	JJ
cord-300796-rmjv56ia	353	11	as	as	IN
cord-300796-rmjv56ia	353	12	the	the	DT
cord-300796-rmjv56ia	353	13	p62	p62	NN
cord-300796-rmjv56ia	353	14	signal	signal	NN
cord-300796-rmjv56ia	353	15	sequence	sequence	NN
cord-300796-rmjv56ia	353	16	was	be	VBD
cord-300796-rmjv56ia	353	17	found	find	VBN
cord-300796-rmjv56ia	353	18	to	to	TO
cord-300796-rmjv56ia	353	19	be	be	VB
cord-300796-rmjv56ia	353	20	glycosylated	glycosylate	VBN
cord-300796-rmjv56ia	353	21	early	early	RB
cord-300796-rmjv56ia	353	22	during	during	IN
cord-300796-rmjv56ia	353	23	translation	translation	NN
cord-300796-rmjv56ia	353	24	of	of	IN
cord-300796-rmjv56ia	353	25	both	both	CC
cord-300796-rmjv56ia	353	26	the	the	DT
cord-300796-rmjv56ia	353	27	p62	p62	NN
cord-300796-rmjv56ia	353	28	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	353	29	as	as	RB
cord-300796-rmjv56ia	353	30	well	well	RB
cord-300796-rmjv56ia	353	31	as	as	IN
cord-300796-rmjv56ia	353	32	the	the	DT
cord-300796-rmjv56ia	353	33	signal	signal	JJ
cord-300796-rmjv56ia	353	34	sequence	sequence	NN
cord-300796-rmjv56ia	353	35	-	-	HYPH
cord-300796-rmjv56ia	353	36	dhfr	dhfr	NN
cord-300796-rmjv56ia	353	37	hybrid	hybrid	NN
cord-300796-rmjv56ia	353	38	chain	chain	NN
cord-300796-rmjv56ia	353	39	.	.	.
cord-300796-rmjv56ia	354	1	Evidence	evidence	NN
cord-300796-rmjv56ia	354	2	for	for	IN
cord-300796-rmjv56ia	354	3	an	an	DT
cord-300796-rmjv56ia	354	4	autoprotease	autoprotease	JJ
cord-300796-rmjv56ia	354	5	activity	activity	NN
cord-300796-rmjv56ia	354	6	of	of	IN
cord-300796-rmjv56ia	354	7	Sindbis	Sindbis	NNP
cord-300796-rmjv56ia	354	8	virus	virus	NN
cord-300796-rmjv56ia	354	9	capsid	capsid	NN
cord-300796-rmjv56ia	354	10	protein	protein	NN
cord-300796-rmjv56ia	354	11	Characterization	characterization	NN
cord-300796-rmjv56ia	354	12	of	of	IN
cord-300796-rmjv56ia	354	13	the	the	DT
cord-300796-rmjv56ia	354	14	inteffacial	inteffacial	JJ
cord-300796-rmjv56ia	354	15	behavior	behavior	NN
cord-300796-rmjv56ia	354	16	and	and	CC
cord-300796-rmjv56ia	354	17	structure	structure	NN
cord-300796-rmjv56ia	354	18	of	of	IN
cord-300796-rmjv56ia	354	19	the	the	DT
cord-300796-rmjv56ia	354	20	signal	signal	JJ
cord-300796-rmjv56ia	354	21	sequence	sequence	NN
cord-300796-rmjv56ia	354	22	of	of	IN
cord-300796-rmjv56ia	354	23	Escherichia	Escherichia	NNP
cord-300796-rmjv56ia	354	24	coli	coli	VBZ
cord-300796-rmjv56ia	354	25	outer	outer	JJ
cord-300796-rmjv56ia	354	26	membrane	membrane	NN
cord-300796-rmjv56ia	354	27	pore	pore	NN
cord-300796-rmjv56ia	354	28	protein	protein	NN
cord-300796-rmjv56ia	354	29	PhoE	PhoE	NNP
cord-300796-rmjv56ia	354	30	Addition	Addition	NNP
cord-300796-rmjv56ia	354	31	of	of	IN
cord-300796-rmjv56ia	354	32	glucosamine	glucosamine	NN
cord-300796-rmjv56ia	354	33	and	and	CC
cord-300796-rmjv56ia	354	34	mannose	mannose	NN
cord-300796-rmjv56ia	354	35	to	to	IN
cord-300796-rmjv56ia	354	36	nascent	nascent	JJ
cord-300796-rmjv56ia	354	37	immunoglobin	immunoglobin	NNP
cord-300796-rmjv56ia	354	38	heavy	heavy	JJ
cord-300796-rmjv56ia	354	39	chains	chain	NNS
cord-300796-rmjv56ia	355	1	A	a	DT
cord-300796-rmjv56ia	355	2	rapid	rapid	JJ
cord-300796-rmjv56ia	355	3	alkaline	alkaline	JJ
cord-300796-rmjv56ia	355	4	extraction	extraction	NN
cord-300796-rmjv56ia	355	5	procedure	procedure	NN
cord-300796-rmjv56ia	355	6	for	for	IN
cord-300796-rmjv56ia	355	7	screening	screen	VBG
cord-300796-rmjv56ia	355	8	recombinant	recombinant	JJ
cord-300796-rmjv56ia	355	9	plasmid	plasmid	NN
cord-300796-rmjv56ia	355	10	DNA	DNA	NNP
cord-300796-rmjv56ia	355	11	Transfer	Transfer	NNP
cord-300796-rmjv56ia	355	12	of	of	IN
cord-300796-rmjv56ia	355	13	proteins	protein	NNS
cord-300796-rmjv56ia	355	14	across	across	IN
cord-300796-rmjv56ia	355	15	membranes	membrane	NNS
cord-300796-rmjv56ia	355	16	.	.	.
cord-300796-rmjv56ia	356	1	I.	I.	NNP
cord-300796-rmjv56ia	356	2	Presence	Presence	NNP
cord-300796-rmjv56ia	356	3	of	of	IN
cord-300796-rmjv56ia	356	4	proteolytically	proteolytically	RB
cord-300796-rmjv56ia	356	5	processed	process	VBN
cord-300796-rmjv56ia	356	6	and	and	CC
cord-300796-rmjv56ia	356	7	unprocessed	unprocessed	JJ
cord-300796-rmjv56ia	356	8	nascent	nascent	JJ
cord-300796-rmjv56ia	356	9	immunoglobin	immunoglobin	NNP
cord-300796-rmjv56ia	356	10	light	light	JJ
cord-300796-rmjv56ia	356	11	chains	chain	NNS
cord-300796-rmjv56ia	356	12	on	on	IN
cord-300796-rmjv56ia	356	13	membrane	membrane	NN
cord-300796-rmjv56ia	356	14	-	-	HYPH
cord-300796-rmjv56ia	356	15	bound	bind	VBN
cord-300796-rmjv56ia	356	16	ribosomes	ribosome	NNS
cord-300796-rmjv56ia	356	17	of	of	IN
cord-300796-rmjv56ia	356	18	murine	murine	JJ
cord-300796-rmjv56ia	356	19	myeloma	myeloma	NN
cord-300796-rmjv56ia	356	20	Controlled	control	VBN
cord-300796-rmjv56ia	356	21	proteolysis	proteolysis	NN
cord-300796-rmjv56ia	356	22	of	of	IN
cord-300796-rmjv56ia	356	23	nascent	nascent	JJ
cord-300796-rmjv56ia	356	24	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	356	25	in	in	IN
cord-300796-rmjv56ia	356	26	rat	rat	NN
cord-300796-rmjv56ia	356	27	liver	liver	NN
cord-300796-rmjv56ia	356	28	cell	cell	NN
cord-300796-rmjv56ia	356	29	fractions	fraction	NNS
cord-300796-rmjv56ia	356	30	.	.	.
cord-300796-rmjv56ia	357	1	I.	I.	NNP
cord-300796-rmjv56ia	357	2	Location	Location	NNP
cord-300796-rmjv56ia	357	3	of	of	IN
cord-300796-rmjv56ia	357	4	the	the	DT
cord-300796-rmjv56ia	357	5	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	357	6	within	within	IN
cord-300796-rmjv56ia	357	7	ribosomes	ribosome	NNS
cord-300796-rmjv56ia	357	8	Role	role	NN
cord-300796-rmjv56ia	357	9	of	of	IN
cord-300796-rmjv56ia	357	10	signal	signal	NN
cord-300796-rmjv56ia	357	11	recognition	recognition	NN
cord-300796-rmjv56ia	357	12	particle	particle	NN
cord-300796-rmjv56ia	357	13	in	in	IN
cord-300796-rmjv56ia	357	14	the	the	DT
cord-300796-rmjv56ia	357	15	membrane	membrane	NN
cord-300796-rmjv56ia	357	16	assembly	assembly	NN
cord-300796-rmjv56ia	357	17	of	of	IN
cord-300796-rmjv56ia	357	18	Sindbis	Sindbis	NNP
cord-300796-rmjv56ia	357	19	viral	viral	JJ
cord-300796-rmjv56ia	357	20	glycoproteins	glycoprotein	NNS
cord-300796-rmjv56ia	357	21	.	.	.
cord-300796-rmjv56ia	358	1	Fur	Fur	NNP
cord-300796-rmjv56ia	358	2	Ribosomemembrane	Ribosomemembrane	NNP
cord-300796-rmjv56ia	358	3	interaction	interaction	NN
cord-300796-rmjv56ia	358	4	:	:	:
cord-300796-rmjv56ia	359	1	in	in	IN
cord-300796-rmjv56ia	359	2	vitro	vitro	FW
cord-300796-rmjv56ia	359	3	binding	binding	NN
cord-300796-rmjv56ia	359	4	of	of	IN
cord-300796-rmjv56ia	359	5	ribosomes	ribosome	NNS
cord-300796-rmjv56ia	359	6	to	to	IN
cord-300796-rmjv56ia	359	7	microsomal	microsomal	NNP
cord-300796-rmjv56ia	359	8	membranes	membrane	NNS
cord-300796-rmjv56ia	359	9	NH2-terminal	NH2-terminal	NNP
cord-300796-rmjv56ia	359	10	hydrophobic	hydrophobic	JJ
cord-300796-rmjv56ia	359	11	region	region	NN
cord-300796-rmjv56ia	359	12	of	of	IN
cord-300796-rmjv56ia	360	1	influenza	influenza	NNP
cord-300796-rmjv56ia	360	2	virus	virus	NN
cord-300796-rmjv56ia	360	3	neuraminidase	neuraminidase	NN
cord-300796-rmjv56ia	360	4	provides	provide	VBZ
cord-300796-rmjv56ia	360	5	the	the	DT
cord-300796-rmjv56ia	360	6	signal	signal	JJ
cord-300796-rmjv56ia	360	7	function	function	NN
cord-300796-rmjv56ia	360	8	in	in	IN
cord-300796-rmjv56ia	360	9	translocation	translocation	NN
cord-300796-rmjv56ia	361	1	In	in	IN
cord-300796-rmjv56ia	361	2	vivo	vivo	NN
cord-300796-rmjv56ia	361	3	function	function	NN
cord-300796-rmjv56ia	361	4	and	and	CC
cord-300796-rmjv56ia	361	5	membrane	membrane	NN
cord-300796-rmjv56ia	361	6	binding	binding	NN
cord-300796-rmjv56ia	361	7	properties	property	NNS
cord-300796-rmjv56ia	361	8	are	be	VBP
cord-300796-rmjv56ia	361	9	correlated	correlate	VBN
cord-300796-rmjv56ia	361	10	for	for	IN
cord-300796-rmjv56ia	361	11	Escherichia	escherichia	NN
cord-300796-rmjv56ia	361	12	coli	coli	NNS
cord-300796-rmjv56ia	361	13	LamB	LamB	NNP
cord-300796-rmjv56ia	361	14	signal	signal	NN
cord-300796-rmjv56ia	361	15	peptides	peptide	NNS
cord-300796-rmjv56ia	361	16	Conformations	Conformations	NNPS
cord-300796-rmjv56ia	361	17	of	of	IN
cord-300796-rmjv56ia	361	18	signal	signal	NN
cord-300796-rmjv56ia	361	19	peptides	peptide	NNS
cord-300796-rmjv56ia	361	20	induced	induce	VBN
cord-300796-rmjv56ia	361	21	by	by	IN
cord-300796-rmjv56ia	361	22	lipids	lipid	NNS
cord-300796-rmjv56ia	361	23	suggest	suggest	VBP
cord-300796-rmjv56ia	361	24	initial	initial	JJ
cord-300796-rmjv56ia	361	25	steps	step	NNS
cord-300796-rmjv56ia	361	26	in	in	IN
cord-300796-rmjv56ia	361	27	protein	protein	NN
cord-300796-rmjv56ia	361	28	export	export	NN
cord-300796-rmjv56ia	361	29	Phenotypic	Phenotypic	NNP
cord-300796-rmjv56ia	361	30	expression	expression	NN
cord-300796-rmjv56ia	361	31	in	in	IN
cord-300796-rmjv56ia	361	32	E.	E.	NNP
cord-300796-rmjv56ia	361	33	coli	coli	VBZ
cord-300796-rmjv56ia	361	34	ofa	ofa	DT
cord-300796-rmjv56ia	361	35	DNA	dna	NN
cord-300796-rmjv56ia	361	36	sequence	sequence	NN
cord-300796-rmjv56ia	361	37	coding	code	VBG
cord-300796-rmjv56ia	361	38	for	for	IN
cord-300796-rmjv56ia	361	39	mouse	mouse	NN
cord-300796-rmjv56ia	361	40	dihydrofolate	dihydrofolate	NNP
cord-300796-rmjv56ia	361	41	reductase	reductase	NNP
cord-300796-rmjv56ia	361	42	Nucleotide	Nucleotide	NNP
cord-300796-rmjv56ia	361	43	sequence	sequence	NN
cord-300796-rmjv56ia	361	44	of	of	IN
cord-300796-rmjv56ia	361	45	the	the	DT
cord-300796-rmjv56ia	361	46	genome	genome	NN
cord-300796-rmjv56ia	361	47	region	region	NN
cord-300796-rmjv56ia	361	48	encoding	encode	VBG
cord-300796-rmjv56ia	361	49	the	the	DT
cord-300796-rmjv56ia	361	50	26S	26S	NNP
cord-300796-rmjv56ia	361	51	mRNA	mRNA	NNP
cord-300796-rmjv56ia	361	52	of	of	IN
cord-300796-rmjv56ia	361	53	eastern	eastern	JJ
cord-300796-rmjv56ia	361	54	equine	equine	JJ
cord-300796-rmjv56ia	361	55	encephalomyelitis	encephalomyelitis	NN
cord-300796-rmjv56ia	361	56	virus	virus	NN
cord-300796-rmjv56ia	361	57	and	and	CC
cord-300796-rmjv56ia	361	58	the	the	DT
cord-300796-rmjv56ia	361	59	deduced	deduce	VBN
cord-300796-rmjv56ia	361	60	amino	amino	NN
cord-300796-rmjv56ia	361	61	acid	acid	NN
cord-300796-rmjv56ia	361	62	sequence	sequence	NN
cord-300796-rmjv56ia	361	63	of	of	IN
cord-300796-rmjv56ia	361	64	the	the	DT
cord-300796-rmjv56ia	361	65	viral	viral	JJ
cord-300796-rmjv56ia	361	66	structural	structural	JJ
cord-300796-rmjv56ia	361	67	proteins	protein	NNS
cord-300796-rmjv56ia	361	68	Conformations	Conformations	NNPS
cord-300796-rmjv56ia	361	69	and	and	CC
cord-300796-rmjv56ia	361	70	orientations	orientation	NNS
cord-300796-rmjv56ia	361	71	of	of	IN
cord-300796-rmjv56ia	361	72	a	a	DT
cord-300796-rmjv56ia	361	73	signal	signal	NN
cord-300796-rmjv56ia	361	74	peptide	peptide	NN
cord-300796-rmjv56ia	361	75	interacting	interact	VBG
cord-300796-rmjv56ia	361	76	with	with	IN
cord-300796-rmjv56ia	361	77	phospholipid	phospholipid	NN
cord-300796-rmjv56ia	361	78	monolayers	monolayer	NNS
cord-300796-rmjv56ia	361	79	Structure	Structure	NNP
cord-300796-rmjv56ia	361	80	of	of	IN
cord-300796-rmjv56ia	361	81	amplified	amplify	VBD
cord-300796-rmjv56ia	361	82	normal	normal	JJ
cord-300796-rmjv56ia	361	83	and	and	CC
cord-300796-rmjv56ia	361	84	variant	variant	JJ
cord-300796-rmjv56ia	361	85	dihydrofolate	dihydrofolate	NNP
cord-300796-rmjv56ia	361	86	reductase	reductase	NN
cord-300796-rmjv56ia	361	87	genes	gene	NNS
cord-300796-rmjv56ia	361	88	in	in	IN
cord-300796-rmjv56ia	361	89	mouse	mouse	NN
cord-300796-rmjv56ia	361	90	sarcoma	sarcoma	NN
cord-300796-rmjv56ia	361	91	SIS0	sis0	CD
cord-300796-rmjv56ia	361	92	cells	cell	NNS
cord-300796-rmjv56ia	361	93	Mutants	mutant	NNS
cord-300796-rmjv56ia	361	94	of	of	IN
cord-300796-rmjv56ia	361	95	the	the	DT
cord-300796-rmjv56ia	361	96	membrane	membrane	NN
cord-300796-rmjv56ia	361	97	-	-	HYPH
cord-300796-rmjv56ia	361	98	binding	bind	VBG
cord-300796-rmjv56ia	361	99	region	region	NN
cord-300796-rmjv56ia	361	100	of	of	IN
cord-300796-rmjv56ia	361	101	Semliki	Semliki	NNP
cord-300796-rmjv56ia	361	102	Forest	Forest	NNP
cord-300796-rmjv56ia	361	103	virus	virus	NN
cord-300796-rmjv56ia	361	104	E2	E2	NNP
cord-300796-rmjv56ia	361	105	protein	protein	NN
cord-300796-rmjv56ia	361	106	.	.	.
cord-300796-rmjv56ia	362	1	I.	I.	NNP
cord-300796-rmjv56ia	362	2	Cell	cell	NN
cord-300796-rmjv56ia	362	3	surface	surface	NN
cord-300796-rmjv56ia	362	4	transport	transport	NN
cord-300796-rmjv56ia	362	5	and	and	CC
cord-300796-rmjv56ia	362	6	fusogenic	fusogenic	JJ
cord-300796-rmjv56ia	362	7	activity	activity	NN
cord-300796-rmjv56ia	362	8	Ross	Ross	NNP
cord-300796-rmjv56ia	362	9	river	river	NN
cord-300796-rmjv56ia	362	10	virus	virus	NN
cord-300796-rmjv56ia	362	11	26	26	CD
cord-300796-rmjv56ia	362	12	S	S	NNP
cord-300796-rmjv56ia	362	13	RNA	RNA	NNP
cord-300796-rmjv56ia	362	14	:	:	:
cord-300796-rmjv56ia	362	15	complete	complete	JJ
cord-300796-rmjv56ia	362	16	nucleotide	nucleotide	JJ
cord-300796-rmjv56ia	362	17	sequence	sequence	NN
cord-300796-rmjv56ia	362	18	and	and	CC
cord-300796-rmjv56ia	362	19	decoded	decode	VBD
cord-300796-rmjv56ia	362	20	sequence	sequence	NN
cord-300796-rmjv56ia	362	21	of	of	IN
cord-300796-rmjv56ia	362	22	the	the	DT
cord-300796-rmjv56ia	362	23	encoded	encode	VBN
cord-300796-rmjv56ia	362	24	structural	structural	JJ
cord-300796-rmjv56ia	362	25	proteins	protein	NNS
cord-300796-rmjv56ia	362	26	Dissection	dissection	NN
cord-300796-rmjv56ia	362	27	of	of	IN
cord-300796-rmjv56ia	362	28	Semliki	Semliki	NNP
cord-300796-rmjv56ia	362	29	Forest	Forest	NNP
cord-300796-rmjv56ia	362	30	virus	virus	NN
cord-300796-rmjv56ia	362	31	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	362	32	delivery	delivery	NN
cord-300796-rmjv56ia	362	33	from	from	IN
cord-300796-rmjv56ia	362	34	the	the	DT
cord-300796-rmjv56ia	362	35	trans	trans	NNP
cord-300796-rmjv56ia	362	36	-	-	NNP
cord-300796-rmjv56ia	362	37	Golgi	Golgi	NNP
cord-300796-rmjv56ia	362	38	network	network	NN
cord-300796-rmjv56ia	362	39	to	to	IN
cord-300796-rmjv56ia	362	40	the	the	DT
cord-300796-rmjv56ia	362	41	cell	cell	NN
cord-300796-rmjv56ia	362	42	surface	surface	NN
cord-300796-rmjv56ia	362	43	in	in	IN
cord-300796-rmjv56ia	362	44	permeabilized	permeabilized	JJ
cord-300796-rmjv56ia	362	45	BHK	BHK	NNP
cord-300796-rmjv56ia	362	46	cells	cell	NNS
cord-300796-rmjv56ia	362	47	Importance	importance	NN
cord-300796-rmjv56ia	362	48	of	of	IN
cord-300796-rmjv56ia	362	49	secondary	secondary	JJ
cord-300796-rmjv56ia	362	50	structure	structure	NN
cord-300796-rmjv56ia	362	51	in	in	IN
cord-300796-rmjv56ia	362	52	the	the	DT
cord-300796-rmjv56ia	362	53	signal	signal	JJ
cord-300796-rmjv56ia	362	54	sequence	sequence	NN
cord-300796-rmjv56ia	362	55	for	for	IN
cord-300796-rmjv56ia	362	56	protein	protein	NN
cord-300796-rmjv56ia	362	57	secretion	secretion	NN
cord-300796-rmjv56ia	363	1	The	the	DT
cord-300796-rmjv56ia	363	2	spontaneous	spontaneous	JJ
cord-300796-rmjv56ia	363	3	insertion	insertion	NN
cord-300796-rmjv56ia	363	4	of	of	IN
cord-300796-rmjv56ia	363	5	proteins	protein	NNS
cord-300796-rmjv56ia	363	6	into	into	IN
cord-300796-rmjv56ia	363	7	and	and	CC
cord-300796-rmjv56ia	363	8	across	across	IN
cord-300796-rmjv56ia	363	9	membranes	membrane	NNS
cord-300796-rmjv56ia	363	10	:	:	:
cord-300796-rmjv56ia	363	11	the	the	DT
cord-300796-rmjv56ia	363	12	helical	helical	JJ
cord-300796-rmjv56ia	363	13	hairpin	hairpin	NN
cord-300796-rmjv56ia	363	14	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	363	15	Solid	solid	JJ
cord-300796-rmjv56ia	363	16	phase	phase	NN
cord-300796-rmjv56ia	363	17	peptide	peptide	NN
cord-300796-rmjv56ia	363	18	synthesis	synthesis	NN
cord-300796-rmjv56ia	363	19	Assembly	Assembly	NNP
cord-300796-rmjv56ia	363	20	of	of	IN
cord-300796-rmjv56ia	363	21	the	the	DT
cord-300796-rmjv56ia	363	22	Semliki	Semliki	NNP
cord-300796-rmjv56ia	363	23	Forest	Forest	NNP
cord-300796-rmjv56ia	363	24	virus	virus	NN
cord-300796-rmjv56ia	363	25	membrane	membrane	NN
cord-300796-rmjv56ia	363	26	glycoproteins	glycoprotein	NNS
cord-300796-rmjv56ia	363	27	in	in	IN
cord-300796-rmjv56ia	363	28	the	the	DT
cord-300796-rmjv56ia	363	29	membrane	membrane	NN
cord-300796-rmjv56ia	363	30	of	of	IN
cord-300796-rmjv56ia	363	31	the	the	DT
cord-300796-rmjv56ia	363	32	endoplasmic	endoplasmic	NN
cord-300796-rmjv56ia	363	33	reticulum	reticulum	NN
cord-300796-rmjv56ia	363	34	in	in	IN
cord-300796-rmjv56ia	363	35	vitro	vitro	FW
cord-300796-rmjv56ia	363	36	Nucleotide	nucleotide	JJ
cord-300796-rmjv56ia	363	37	sequence	sequence	NN
cord-300796-rmjv56ia	363	38	of	of	IN
cord-300796-rmjv56ia	363	39	eDNA	edna	NN
cord-300796-rmjv56ia	363	40	coding	code	VBG
cord-300796-rmjv56ia	363	41	for	for	IN
cord-300796-rmjv56ia	363	42	Semliki	Semliki	NNP
cord-300796-rmjv56ia	363	43	Forest	Forest	NNP
cord-300796-rmjv56ia	363	44	virus	virus	NN
cord-300796-rmjv56ia	363	45	membrane	membrane	NN
cord-300796-rmjv56ia	363	46	glycoproteins	glycoprotein	NNS
cord-300796-rmjv56ia	363	47	Structure	Structure	NNP
cord-300796-rmjv56ia	363	48	and	and	CC
cord-300796-rmjv56ia	363	49	assembly	assembly	NN
cord-300796-rmjv56ia	363	50	of	of	IN
cord-300796-rmjv56ia	363	51	alphaviruses	alphaviruses	NNP
cord-300796-rmjv56ia	363	52	Expression	Expression	NNP
cord-300796-rmjv56ia	363	53	of	of	IN
cord-300796-rmjv56ia	363	54	Semliki	Semliki	NNP
cord-300796-rmjv56ia	363	55	Forest	Forest	NNP
cord-300796-rmjv56ia	363	56	virus	virus	NN
cord-300796-rmjv56ia	363	57	proteins	protein	VBZ
cord-300796-rmjv56ia	363	58	from	from	IN
cord-300796-rmjv56ia	363	59	cloned	clone	VBN
cord-300796-rmjv56ia	363	60	complementary	complementary	JJ
cord-300796-rmjv56ia	363	61	DNA	dna	NN
cord-300796-rmjv56ia	363	62	.	.	.
cord-300796-rmjv56ia	364	1	II	II	NNP
cord-300796-rmjv56ia	364	2	.	.	.
cord-300796-rmjv56ia	365	1	The	the	DT
cord-300796-rmjv56ia	365	2	membrane	membrane	NN
cord-300796-rmjv56ia	365	3	-	-	HYPH
cord-300796-rmjv56ia	365	4	spanning	span	VBG
cord-300796-rmjv56ia	365	5	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	365	6	E2	E2	NNP
cord-300796-rmjv56ia	365	7	is	be	VBZ
cord-300796-rmjv56ia	365	8	transported	transport	VBN
cord-300796-rmjv56ia	365	9	to	to	IN
cord-300796-rmjv56ia	365	10	the	the	DT
cord-300796-rmjv56ia	365	11	cell	cell	NN
cord-300796-rmjv56ia	365	12	surface	surface	NN
cord-300796-rmjv56ia	365	13	without	without	IN
cord-300796-rmjv56ia	365	14	its	-PRON-	PRP$
cord-300796-rmjv56ia	365	15	normal	normal	JJ
cord-300796-rmjv56ia	365	16	cytoplasmic	cytoplasmic	JJ
cord-300796-rmjv56ia	365	17	domain	domain	NN
cord-300796-rmjv56ia	365	18	Glycosylation	Glycosylation	NNP
cord-300796-rmjv56ia	365	19	site	site	NN
cord-300796-rmjv56ia	365	20	binding	bind	VBG
cord-300796-rmjv56ia	365	21	protein	protein	NN
cord-300796-rmjv56ia	365	22	,	,	,
cord-300796-rmjv56ia	365	23	a	a	DT
cord-300796-rmjv56ia	365	24	component	component	NN
cord-300796-rmjv56ia	365	25	of	of	IN
cord-300796-rmjv56ia	365	26	oligosaccharyl	oligosaccharyl	NNP
cord-300796-rmjv56ia	365	27	transferase	transferase	NNP
cord-300796-rmjv56ia	365	28	,	,	,
cord-300796-rmjv56ia	365	29	is	be	VBZ
cord-300796-rmjv56ia	365	30	highly	highly	RB
cord-300796-rmjv56ia	365	31	similar	similar	JJ
cord-300796-rmjv56ia	365	32	to	to	IN
cord-300796-rmjv56ia	365	33	three	three	CD
cord-300796-rmjv56ia	365	34	other	other	JJ
cord-300796-rmjv56ia	365	35	57	57	CD
cord-300796-rmjv56ia	365	36	kD	kd	JJ
cord-300796-rmjv56ia	365	37	luminal	luminal	JJ
cord-300796-rmjv56ia	365	38	proteins	protein	NNS
cord-300796-rmjv56ia	365	39	of	of	IN
cord-300796-rmjv56ia	365	40	the	the	DT
cord-300796-rmjv56ia	365	41	ER	ER	NNP
cord-300796-rmjv56ia	365	42	Synthesis	Synthesis	NNP
cord-300796-rmjv56ia	365	43	of	of	IN
cord-300796-rmjv56ia	365	44	Ranscher	Ranscher	NNP
cord-300796-rmjv56ia	365	45	murine	murine	JJ
cord-300796-rmjv56ia	365	46	leukemia	leukemia	NN
cord-300796-rmjv56ia	365	47	virus	virus	NN
cord-300796-rmjv56ia	365	48	-	-	HYPH
cord-300796-rmjv56ia	365	49	specific	specific	JJ
cord-300796-rmjv56ia	365	50	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	365	51	in	in	IN
cord-300796-rmjv56ia	365	52	vitro	vitro	FW
cord-300796-rmjv56ia	365	53	Translocation	translocation	NN
cord-300796-rmjv56ia	365	54	of	of	IN
cord-300796-rmjv56ia	365	55	secretory	secretory	JJ
cord-300796-rmjv56ia	365	56	proteins	protein	NNS
cord-300796-rmjv56ia	365	57	across	across	IN
cord-300796-rmjv56ia	365	58	the	the	DT
cord-300796-rmjv56ia	365	59	microsomal	microsomal	NNP
cord-300796-rmjv56ia	365	60	membrane	membrane	NN
cord-300796-rmjv56ia	365	61	occurs	occur	VBZ
cord-300796-rmjv56ia	365	62	through	through	IN
cord-300796-rmjv56ia	365	63	an	an	DT
cord-300796-rmjv56ia	365	64	environment	environment	NN
cord-300796-rmjv56ia	365	65	accessible	accessible	JJ
cord-300796-rmjv56ia	365	66	to	to	IN
cord-300796-rmjv56ia	365	67	aqueous	aqueous	JJ
cord-300796-rmjv56ia	365	68	perturbants	perturbant	NNS
cord-300796-rmjv56ia	365	69	Glycosylation	Glycosylation	NNP
cord-300796-rmjv56ia	365	70	of	of	IN
cord-300796-rmjv56ia	365	71	ovalbumin	ovalbumin	FW
cord-300796-rmjv56ia	365	72	nascent	nascent	JJ
cord-300796-rmjv56ia	365	73	chains	chain	NNS
cord-300796-rmjv56ia	365	74	:	:	:
cord-300796-rmjv56ia	365	75	the	the	DT
cord-300796-rmjv56ia	365	76	spatial	spatial	JJ
cord-300796-rmjv56ia	365	77	relationship	relationship	NN
cord-300796-rmjv56ia	365	78	between	between	IN
cord-300796-rmjv56ia	365	79	translation	translation	NN
cord-300796-rmjv56ia	365	80	and	and	CC
cord-300796-rmjv56ia	365	81	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	365	82	Sequence	sequence	NN
cord-300796-rmjv56ia	365	83	analysis	analysis	NN
cord-300796-rmjv56ia	365	84	of	of	IN
cord-300796-rmjv56ia	365	85	three	three	CD
cord-300796-rmjv56ia	365	86	Sindbis	Sindbis	NNP
cord-300796-rmjv56ia	365	87	virus	virus	NN
cord-300796-rmjv56ia	365	88	mutants	mutant	NNS
cord-300796-rmjv56ia	365	89	temperature	temperature	NN
cord-300796-rmjv56ia	365	90	-	-	HYPH
cord-300796-rmjv56ia	365	91	sensitive	sensitive	JJ
cord-300796-rmjv56ia	365	92	in	in	IN
cord-300796-rmjv56ia	365	93	the	the	DT
cord-300796-rmjv56ia	365	94	capsid	capsid	JJ
cord-300796-rmjv56ia	365	95	protein	protein	NN
cord-300796-rmjv56ia	365	96	autoprotease	autoprotease	NN
cord-300796-rmjv56ia	365	97	Evidence	evidence	NN
cord-300796-rmjv56ia	365	98	for	for	IN
cord-300796-rmjv56ia	365	99	a	a	DT
cord-300796-rmjv56ia	365	100	separate	separate	JJ
cord-300796-rmjv56ia	365	101	signal	signal	NN
cord-300796-rmjv56ia	365	102	sequence	sequence	NN
cord-300796-rmjv56ia	365	103	for	for	IN
cord-300796-rmjv56ia	365	104	the	the	DT
cord-300796-rmjv56ia	365	105	carboxy	carboxy	JJ
cord-300796-rmjv56ia	365	106	-	-	HYPH
cord-300796-rmjv56ia	365	107	terminal	terminal	NN
cord-300796-rmjv56ia	365	108	envelope	envelope	NN
cord-300796-rmjv56ia	365	109	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	365	110	E1	e1	NN
cord-300796-rmjv56ia	365	111	of	of	IN
cord-300796-rmjv56ia	365	112	Semliki	Semliki	NNP
cord-300796-rmjv56ia	365	113	Forest	Forest	NNP
cord-300796-rmjv56ia	365	114	virus	virus	NN
cord-300796-rmjv56ia	365	115	Preparation	preparation	NN
cord-300796-rmjv56ia	365	116	and	and	CC
cord-300796-rmjv56ia	365	117	use	use	NN
cord-300796-rmjv56ia	365	118	of	of	IN
cord-300796-rmjv56ia	365	119	nuclease	nuclease	NN
cord-300796-rmjv56ia	365	120	-	-	HYPH
cord-300796-rmjv56ia	365	121	treated	treat	VBN
cord-300796-rmjv56ia	365	122	rabbit	rabbit	NN
cord-300796-rmjv56ia	365	123	reticulocyte	reticulocyte	NNP
cord-300796-rmjv56ia	365	124	lysates	lysates	NNP
cord-300796-rmjv56ia	365	125	for	for	IN
cord-300796-rmjv56ia	365	126	the	the	DT
cord-300796-rmjv56ia	365	127	translation	translation	NN
cord-300796-rmjv56ia	365	128	of	of	IN
cord-300796-rmjv56ia	365	129	eucaryotic	eucaryotic	JJ
cord-300796-rmjv56ia	365	130	messenger	messenger	NN
cord-300796-rmjv56ia	365	131	RNA	RNA	NNP
cord-300796-rmjv56ia	365	132	Dog	Dog	NNP
cord-300796-rmjv56ia	365	133	pancreatic	pancreatic	NN
cord-300796-rmjv56ia	365	134	microsomalmembrane	microsomalmembrane	NN
cord-300796-rmjv56ia	365	135	polypeptides	polypeptide	NNS
cord-300796-rmjv56ia	365	136	analysed	analyse	VBN
cord-300796-rmjv56ia	365	137	by	by	IN
cord-300796-rmjv56ia	365	138	two	two	CD
cord-300796-rmjv56ia	365	139	-	-	HYPH
cord-300796-rmjv56ia	365	140	dimensional	dimensional	JJ
cord-300796-rmjv56ia	365	141	gel	gel	NN
cord-300796-rmjv56ia	365	142	electrophoresis	electrophoresis	NN
cord-300796-rmjv56ia	365	143	Plasmid	Plasmid	NNP
cord-300796-rmjv56ia	365	144	cloning	clone	VBG
cord-300796-rmjv56ia	365	145	vehicles	vehicle	NNS
cord-300796-rmjv56ia	365	146	derived	derive	VBN
cord-300796-rmjv56ia	365	147	from	from	IN
cord-300796-rmjv56ia	365	148	plasmids	plasmid	NNS
cord-300796-rmjv56ia	365	149	ColE1	ColE1	NNP
cord-300796-rmjv56ia	365	150	,	,	,
cord-300796-rmjv56ia	365	151	F	F	NNP
cord-300796-rmjv56ia	365	152	,	,	,
cord-300796-rmjv56ia	365	153	R6	R6	NNP
cord-300796-rmjv56ia	365	154	K	K	NNP
cord-300796-rmjv56ia	365	155	,	,	,
cord-300796-rmjv56ia	365	156	and	and	CC
cord-300796-rmjv56ia	365	157	RK2	RK2	NNP
cord-300796-rmjv56ia	365	158	Idealization	idealization	NN
cord-300796-rmjv56ia	365	159	of	of	IN
cord-300796-rmjv56ia	365	160	the	the	DT
cord-300796-rmjv56ia	365	161	hydrophobic	hydrophobic	JJ
cord-300796-rmjv56ia	365	162	segment	segment	NN
cord-300796-rmjv56ia	365	163	of	of	IN
cord-300796-rmjv56ia	365	164	the	the	DT
cord-300796-rmjv56ia	365	165	alkaline	alkaline	JJ
cord-300796-rmjv56ia	365	166	phosphatase	phosphatase	NN
cord-300796-rmjv56ia	365	167	signal	signal	NN
cord-300796-rmjv56ia	365	168	peptide	peptide	NN
cord-300796-rmjv56ia	365	169	Nucleotide	nucleotide	JJ
cord-300796-rmjv56ia	365	170	sequence	sequence	NN
cord-300796-rmjv56ia	365	171	of	of	IN
cord-300796-rmjv56ia	365	172	the	the	DT
cord-300796-rmjv56ia	365	173	26	26	CD
cord-300796-rmjv56ia	365	174	S	S	NNP
cord-300796-rmjv56ia	365	175	mRNA	mrna	NN
cord-300796-rmjv56ia	365	176	of	of	IN
cord-300796-rmjv56ia	365	177	the	the	DT
cord-300796-rmjv56ia	365	178	virulent	virulent	JJ
cord-300796-rmjv56ia	365	179	Trinidad	Trinidad	NNP
cord-300796-rmjv56ia	365	180	donkey	donkey	NN
cord-300796-rmjv56ia	365	181	strain	strain	NN
cord-300796-rmjv56ia	365	182	of	of	IN
cord-300796-rmjv56ia	365	183	Venezuelan	venezuelan	JJ
cord-300796-rmjv56ia	365	184	equine	equine	NN
cord-300796-rmjv56ia	365	185	encephalitis	encephalitis	NN
cord-300796-rmjv56ia	365	186	virus	virus	NN
cord-300796-rmjv56ia	365	187	and	and	CC
cord-300796-rmjv56ia	365	188	deduced	deduce	VBD
cord-300796-rmjv56ia	365	189	sequence	sequence	NN
cord-300796-rmjv56ia	365	190	of	of	IN
cord-300796-rmjv56ia	365	191	the	the	DT
cord-300796-rmjv56ia	365	192	encoded	encode	VBN
cord-300796-rmjv56ia	365	193	structural	structural	JJ
cord-300796-rmjv56ia	365	194	proteins	protein	NNS
cord-300796-rmjv56ia	365	195	Expression	Expression	NNP
cord-300796-rmjv56ia	365	196	of	of	IN
cord-300796-rmjv56ia	365	197	Semliki	Semliki	NNP
cord-300796-rmjv56ia	365	198	Forest	Forest	NNP
cord-300796-rmjv56ia	365	199	virus	virus	NN
cord-300796-rmjv56ia	365	200	proteins	protein	VBZ
cord-300796-rmjv56ia	365	201	from	from	IN
cord-300796-rmjv56ia	365	202	cloned	clone	VBN
cord-300796-rmjv56ia	365	203	complementary	complementary	JJ
cord-300796-rmjv56ia	365	204	DNA	dna	NN
cord-300796-rmjv56ia	365	205	.	.	.
cord-300796-rmjv56ia	366	1	I.	I.	NNP
cord-300796-rmjv56ia	367	1	The	the	DT
cord-300796-rmjv56ia	367	2	fusion	fusion	NN
cord-300796-rmjv56ia	367	3	activity	activity	NN
cord-300796-rmjv56ia	367	4	of	of	IN
cord-300796-rmjv56ia	367	5	the	the	DT
cord-300796-rmjv56ia	367	6	spike	spike	NN
cord-300796-rmjv56ia	367	7	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	367	8	Assembly	Assembly	NNP
cord-300796-rmjv56ia	367	9	of	of	IN
cord-300796-rmjv56ia	367	10	asparagine	asparagine	NN
cord-300796-rmjv56ia	367	11	-	-	HYPH
cord-300796-rmjv56ia	367	12	linked	link	VBN
cord-300796-rmjv56ia	367	13	oligosaccharides	oligosaccharide	NNS
cord-300796-rmjv56ia	367	14	Binding	Binding	NNP
cord-300796-rmjv56ia	367	15	of	of	IN
cord-300796-rmjv56ia	367	16	synthetic	synthetic	JJ
cord-300796-rmjv56ia	367	17	clustered	clustered	JJ
cord-300796-rmjv56ia	367	18	ligands	ligand	NNS
cord-300796-rmjv56ia	367	19	to	to	IN
cord-300796-rmjv56ia	367	20	the	the	DT
cord-300796-rmjv56ia	367	21	Gal	Gal	NNP
cord-300796-rmjv56ia	367	22	/	/	SYM
cord-300796-rmjv56ia	367	23	GalNac	GalNac	NNP
cord-300796-rmjv56ia	367	24	lectin	lectin	NN
cord-300796-rmjv56ia	367	25	on	on	IN
cord-300796-rmjv56ia	367	26	isolated	isolate	VBN
cord-300796-rmjv56ia	367	27	rabbit	rabbit	NN
cord-300796-rmjv56ia	367	28	hepatocytes	hepatocyte	NNS
cord-300796-rmjv56ia	368	1	Structural	structural	JJ
cord-300796-rmjv56ia	368	2	and	and	CC
cord-300796-rmjv56ia	368	3	evolutionary	evolutionary	JJ
cord-300796-rmjv56ia	368	4	analysis	analysis	NN
cord-300796-rmjv56ia	368	5	of	of	IN
cord-300796-rmjv56ia	368	6	the	the	DT
cord-300796-rmjv56ia	368	7	two	two	CD
cord-300796-rmjv56ia	368	8	chimpanzee	chimpanzee	NN
cord-300796-rmjv56ia	368	9	alpha	alpha	NN
cord-300796-rmjv56ia	368	10	-	-	HYPH
cord-300796-rmjv56ia	368	11	globin	globin	NN
cord-300796-rmjv56ia	369	1	mRNAs	mRNAs	.
cord-300796-rmjv56ia	370	1	Signal	signal	NN
cord-300796-rmjv56ia	370	2	recognition	recognition	NN
cord-300796-rmjv56ia	370	3	particle	particle	NN
cord-300796-rmjv56ia	370	4	-	-	HYPH
cord-300796-rmjv56ia	370	5	dependent	dependent	JJ
cord-300796-rmjv56ia	370	6	membrane	membrane	NN
cord-300796-rmjv56ia	370	7	insertion	insertion	NN
cord-300796-rmjv56ia	370	8	of	of	IN
cord-300796-rmjv56ia	370	9	mouse	mouse	NN
cord-300796-rmjv56ia	370	10	invariant	invariant	JJ
cord-300796-rmjv56ia	370	11	chain	chain	NN
cord-300796-rmjv56ia	370	12	:	:	:
cord-300796-rmjv56ia	371	1	a	a	DT
cord-300796-rmjv56ia	371	2	membrane	membrane	NN
cord-300796-rmjv56ia	371	3	-	-	HYPH
cord-300796-rmjv56ia	371	4	spanning	span	VBG
cord-300796-rmjv56ia	371	5	protein	protein	NN
cord-300796-rmjv56ia	371	6	with	with	IN
cord-300796-rmjv56ia	371	7	a	a	DT
cord-300796-rmjv56ia	371	8	cytoplasmically	cytoplasmically	RB
cord-300796-rmjv56ia	371	9	exposed	expose	VBN
cord-300796-rmjv56ia	371	10	amino	amino	NN
cord-300796-rmjv56ia	371	11	terminus	terminus	NN
cord-300796-rmjv56ia	371	12	Transport	transport	NN
cord-300796-rmjv56ia	371	13	of	of	IN
cord-300796-rmjv56ia	371	14	secretory	secretory	NN
cord-300796-rmjv56ia	371	15	and	and	CC
cord-300796-rmjv56ia	371	16	membrane	membrane	NN
cord-300796-rmjv56ia	371	17	glycoproteins	glycoprotein	NNS
cord-300796-rmjv56ia	371	18	form	form	VBP
cord-300796-rmjv56ia	371	19	the	the	DT
cord-300796-rmjv56ia	371	20	rough	rough	JJ
cord-300796-rmjv56ia	371	21	endoplasmic	endoplasmic	NN
cord-300796-rmjv56ia	371	22	reticulum	reticulum	NN
cord-300796-rmjv56ia	371	23	to	to	IN
cord-300796-rmjv56ia	371	24	the	the	DT
cord-300796-rmjv56ia	371	25	Golgi	Golgi	NNP
cord-300796-rmjv56ia	371	26	Partial	Partial	NNP
cord-300796-rmjv56ia	371	27	resistance	resistance	NN
cord-300796-rmjv56ia	371	28	Of	of	IN
cord-300796-rmjv56ia	371	29	nascent	nascent	JJ
cord-300796-rmjv56ia	371	30	polypeptide	polypeptide	NN
cord-300796-rmjv56ia	371	31	chains	chain	NNS
cord-300796-rmjv56ia	371	32	to	to	IN
cord-300796-rmjv56ia	371	33	proteolytic	proteolytic	JJ
cord-300796-rmjv56ia	371	34	digestion	digestion	NN
cord-300796-rmjv56ia	371	35	due	due	IN
cord-300796-rmjv56ia	371	36	to	to	IN
cord-300796-rmjv56ia	371	37	ribosomal	ribosomal	JJ
cord-300796-rmjv56ia	371	38	shielding	shield	VBG
cord-300796-rmjv56ia	371	39	Molecular	Molecular	NNP
cord-300796-rmjv56ia	371	40	Cloning	cloning	NN
cord-300796-rmjv56ia	371	41	:	:	:
cord-300796-rmjv56ia	371	42	A	a	DT
cord-300796-rmjv56ia	371	43	Laboratory	Laboratory	NNP
cord-300796-rmjv56ia	371	44	Manual	Manual	NNP
cord-300796-rmjv56ia	371	45	Membrane	Membrane	NNP
cord-300796-rmjv56ia	371	46	integration	integration	NN
cord-300796-rmjv56ia	371	47	and	and	CC
cord-300796-rmjv56ia	371	48	intracellular	intracellular	JJ
cord-300796-rmjv56ia	371	49	transport	transport	NN
cord-300796-rmjv56ia	371	50	of	of	IN
cord-300796-rmjv56ia	371	51	the	the	DT
cord-300796-rmjv56ia	371	52	coronavirus	coronavirus	NN
cord-300796-rmjv56ia	371	53	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	371	54	El	El	NNP
cord-300796-rmjv56ia	371	55	,	,	,
cord-300796-rmjv56ia	371	56	a	a	DT
cord-300796-rmjv56ia	371	57	class	class	NN
cord-300796-rmjv56ia	371	58	Ill	Ill	NNP
cord-300796-rmjv56ia	371	59	membrane	membrane	NN
cord-300796-rmjv56ia	371	60	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	371	61	Biochemical	biochemical	JJ
cord-300796-rmjv56ia	371	62	studies	study	NNS
cord-300796-rmjv56ia	371	63	of	of	IN
cord-300796-rmjv56ia	371	64	the	the	DT
cord-300796-rmjv56ia	371	65	maturation	maturation	NN
cord-300796-rmjv56ia	371	66	of	of	IN
cord-300796-rmjv56ia	371	67	the	the	DT
cord-300796-rmjv56ia	371	68	small	small	JJ
cord-300796-rmjv56ia	371	69	Sindbis	Sindbis	NNP
cord-300796-rmjv56ia	371	70	virus	virus	NN
cord-300796-rmjv56ia	371	71	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	371	72	E3	E3	NNP
cord-300796-rmjv56ia	371	73	Reinitiation	Reinitiation	NNP
cord-300796-rmjv56ia	371	74	of	of	IN
cord-300796-rmjv56ia	371	75	translocation	translocation	NN
cord-300796-rmjv56ia	371	76	in	in	IN
cord-300796-rmjv56ia	371	77	the	the	DT
cord-300796-rmjv56ia	371	78	Semliki	Semliki	NNP
cord-300796-rmjv56ia	371	79	Forest	Forest	NNP
cord-300796-rmjv56ia	371	80	virus	virus	NN
cord-300796-rmjv56ia	371	81	structural	structural	JJ
cord-300796-rmjv56ia	371	82	polyprotein	polyprotein	NN
cord-300796-rmjv56ia	371	83	:	:	:
cord-300796-rmjv56ia	371	84	identification	identification	NN
cord-300796-rmjv56ia	371	85	of	of	IN
cord-300796-rmjv56ia	371	86	the	the	DT
cord-300796-rmjv56ia	371	87	signal	signal	NN
cord-300796-rmjv56ia	371	88	for	for	IN
cord-300796-rmjv56ia	371	89	the	the	DT
cord-300796-rmjv56ia	371	90	El	El	NNP
cord-300796-rmjv56ia	371	91	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	371	92	Processing	processing	NN
cord-300796-rmjv56ia	371	93	of	of	IN
cord-300796-rmjv56ia	371	94	the	the	DT
cord-300796-rmjv56ia	371	95	Semliki	Semliki	NNP
cord-300796-rmjv56ia	371	96	Forest	Forest	NNP
cord-300796-rmjv56ia	371	97	structural	structural	JJ
cord-300796-rmjv56ia	371	98	polyprotein	polyprotein	NN
cord-300796-rmjv56ia	371	99	:	:	:
cord-300796-rmjv56ia	371	100	role	role	NN
cord-300796-rmjv56ia	371	101	of	of	IN
cord-300796-rmjv56ia	371	102	the	the	DT
cord-300796-rmjv56ia	371	103	capsid	capsid	JJ
cord-300796-rmjv56ia	371	104	protease	protease	NN
cord-300796-rmjv56ia	371	105	On	on	IN
cord-300796-rmjv56ia	371	106	the	the	DT
cord-300796-rmjv56ia	371	107	possible	possible	JJ
cord-300796-rmjv56ia	371	108	participation	participation	NN
cord-300796-rmjv56ia	371	109	of	of	IN
cord-300796-rmjv56ia	371	110	acid	acid	JJ
cord-300796-rmjv56ia	371	111	phospholipids	phospholipid	NNS
cord-300796-rmjv56ia	371	112	in	in	IN
cord-300796-rmjv56ia	371	113	the	the	DT
cord-300796-rmjv56ia	371	114	translocation	translocation	NN
cord-300796-rmjv56ia	371	115	of	of	IN
cord-300796-rmjv56ia	371	116	secreted	secrete	VBN
cord-300796-rmjv56ia	371	117	proteins	protein	NNS
cord-300796-rmjv56ia	371	118	through	through	IN
cord-300796-rmjv56ia	371	119	the	the	DT
cord-300796-rmjv56ia	371	120	bacterial	bacterial	JJ
cord-300796-rmjv56ia	371	121	cytoplasmic	cytoplasmic	JJ
cord-300796-rmjv56ia	371	122	membrane	membrane	NN
cord-300796-rmjv56ia	371	123	.	.	.
cord-300796-rmjv56ia	372	1	FEBS	FEBS	NNP
cord-300796-rmjv56ia	372	2	(	(	-LRB-
cord-300796-rmjv56ia	372	3	Fed	Fed	NNP
cord-300796-rmjv56ia	372	4	.	.	.
cord-300796-rmjv56ia	373	1	Fur	Fur	NNP
cord-300796-rmjv56ia	373	2	Structure	Structure	NNP
cord-300796-rmjv56ia	373	3	and	and	CC
cord-300796-rmjv56ia	373	4	genomic	genomic	JJ
cord-300796-rmjv56ia	373	5	organization	organization	NN
cord-300796-rmjv56ia	373	6	of	of	IN
cord-300796-rmjv56ia	373	7	the	the	DT
cord-300796-rmjv56ia	373	8	mouse	mouse	NN
cord-300796-rmjv56ia	373	9	dihydrofolate	dihydrofolate	NNP
cord-300796-rmjv56ia	373	10	reductase	reductase	NN
cord-300796-rmjv56ia	373	11	gene	gene	NN
cord-300796-rmjv56ia	373	12	Translocations	translocation	NNS
cord-300796-rmjv56ia	373	13	of	of	IN
cord-300796-rmjv56ia	373	14	domains	domain	NNS
cord-300796-rmjv56ia	373	15	of	of	IN
cord-300796-rmjv56ia	373	16	nascent	nascent	JJ
cord-300796-rmjv56ia	373	17	periplasmic	periplasmic	JJ
cord-300796-rmjv56ia	373	18	proteins	protein	NNS
cord-300796-rmjv56ia	373	19	across	across	IN
cord-300796-rmjv56ia	373	20	the	the	DT
cord-300796-rmjv56ia	373	21	cytoplasmic	cytoplasmic	JJ
cord-300796-rmjv56ia	373	22	membrane	membrane	NN
cord-300796-rmjv56ia	373	23	is	be	VBZ
cord-300796-rmjv56ia	373	24	independent	independent	JJ
cord-300796-rmjv56ia	373	25	of	of	IN
cord-300796-rmjv56ia	373	26	elongation	elongation	NN
cord-300796-rmjv56ia	373	27	Extensions	extension	NNS
cord-300796-rmjv56ia	373	28	of	of	IN
cord-300796-rmjv56ia	373	29	the	the	DT
cord-300796-rmjv56ia	373	30	signal	signal	JJ
cord-300796-rmjv56ia	373	31	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	373	32	-	-	HYPH
cord-300796-rmjv56ia	373	33	sequential	sequential	JJ
cord-300796-rmjv56ia	373	34	insertion	insertion	NN
cord-300796-rmjv56ia	373	35	model	model	NN
cord-300796-rmjv56ia	373	36	versus	versus	IN
cord-300796-rmjv56ia	373	37	amphipathic	amphipathic	NNP
cord-300796-rmjv56ia	373	38	tunnel	tunnel	NN
cord-300796-rmjv56ia	373	39	hypothesis	hypothesis	NN
cord-300796-rmjv56ia	373	40	.	.	.
cord-300796-rmjv56ia	374	1	FEBS	FEBS	NNP
cord-300796-rmjv56ia	374	2	(	(	-LRB-
cord-300796-rmjv56ia	374	3	Fed	Fed	NNP
cord-300796-rmjv56ia	374	4	.	.	.
cord-300796-rmjv56ia	375	1	Eur	Eur	NNP
cord-300796-rmjv56ia	375	2	Protein	Protein	NNP
cord-300796-rmjv56ia	375	3	translocation	translocation	NN
cord-300796-rmjv56ia	375	4	across	across	IN
cord-300796-rmjv56ia	375	5	and	and	CC
cord-300796-rmjv56ia	375	6	integration	integration	NN
cord-300796-rmjv56ia	375	7	into	into	IN
cord-300796-rmjv56ia	375	8	membranes	membrane	NNS
cord-300796-rmjv56ia	375	9	Improved	improve	VBN
cord-300796-rmjv56ia	375	10	plasmid	plasmid	NN
cord-300796-rmjv56ia	375	11	vectors	vector	NNS
cord-300796-rmjv56ia	375	12	with	with	IN
cord-300796-rmjv56ia	375	13	a	a	DT
cord-300796-rmjv56ia	375	14	thermoinducible	thermoinducible	JJ
cord-300796-rmjv56ia	375	15	expression	expression	NN
cord-300796-rmjv56ia	375	16	and	and	CC
cord-300796-rmjv56ia	375	17	temperature	temperature	NN
cord-300796-rmjv56ia	375	18	-	-	HYPH
cord-300796-rmjv56ia	375	19	regulated	regulate	VBN
cord-300796-rmjv56ia	375	20	runaway	runaway	JJ
cord-300796-rmjv56ia	375	21	replication	replication	NN
cord-300796-rmjv56ia	375	22	Nucleotide	nucleotide	JJ
cord-300796-rmjv56ia	375	23	sequence	sequence	NN
cord-300796-rmjv56ia	375	24	of	of	IN
cord-300796-rmjv56ia	375	25	the	the	DT
cord-300796-rmjv56ia	375	26	26S	26S	NNP
cord-300796-rmjv56ia	375	27	mRNA	mrna	NN
cord-300796-rmjv56ia	375	28	of	of	IN
cord-300796-rmjv56ia	375	29	Sindbis	Sindbis	NNP
cord-300796-rmjv56ia	375	30	virus	virus	NN
cord-300796-rmjv56ia	375	31	and	and	CC
cord-300796-rmjv56ia	375	32	deduced	deduce	VBD
cord-300796-rmjv56ia	375	33	sequence	sequence	NN
cord-300796-rmjv56ia	375	34	of	of	IN
cord-300796-rmjv56ia	375	35	the	the	DT
cord-300796-rmjv56ia	375	36	encoded	encode	VBN
cord-300796-rmjv56ia	375	37	virus	virus	NN
cord-300796-rmjv56ia	375	38	structural	structural	JJ
cord-300796-rmjv56ia	375	39	proteins	protein	NNS
cord-300796-rmjv56ia	375	40	Identification	Identification	NNP
cord-300796-rmjv56ia	375	41	of	of	IN
cord-300796-rmjv56ia	375	42	signal	signal	NN
cord-300796-rmjv56ia	375	43	sequence	sequence	NN
cord-300796-rmjv56ia	375	44	binding	bind	VBG
cord-300796-rmjv56ia	375	45	proteins	protein	NNS
cord-300796-rmjv56ia	375	46	integrated	integrate	VBN
cord-300796-rmjv56ia	375	47	into	into	IN
cord-300796-rmjv56ia	375	48	the	the	DT
cord-300796-rmjv56ia	375	49	rough	rough	JJ
cord-300796-rmjv56ia	375	50	endoplasmic	endoplasmic	NN
cord-300796-rmjv56ia	375	51	reticulum	reticulum	NN
cord-300796-rmjv56ia	375	52	membrane	membrane	NN
cord-300796-rmjv56ia	375	53	Polypeptide	Polypeptide	NNP
cord-300796-rmjv56ia	375	54	chain	chain	NN
cord-300796-rmjv56ia	375	55	binding	bind	VBG
cord-300796-rmjv56ia	375	56	proteins	protein	NNS
cord-300796-rmjv56ia	375	57	:	:	:
cord-300796-rmjv56ia	375	58	catalysts	catalyst	NNS
cord-300796-rmjv56ia	375	59	of	of	IN
cord-300796-rmjv56ia	375	60	protein	protein	NN
cord-300796-rmjv56ia	375	61	folding	folding	NN
cord-300796-rmjv56ia	375	62	and	and	CC
cord-300796-rmjv56ia	375	63	related	related	JJ
cord-300796-rmjv56ia	375	64	processes	process	NNS
cord-300796-rmjv56ia	375	65	in	in	IN
cord-300796-rmjv56ia	375	66	cells	cell	NNS
cord-300796-rmjv56ia	375	67	Synchronized	synchronize	VBD
cord-300796-rmjv56ia	375	68	transmembrane	transmembrane	NN
cord-300796-rmjv56ia	375	69	insertion	insertion	NN
cord-300796-rmjv56ia	375	70	and	and	CC
cord-300796-rmjv56ia	375	71	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	375	72	of	of	IN
cord-300796-rmjv56ia	375	73	a	a	DT
cord-300796-rmjv56ia	375	74	nascent	nascent	JJ
cord-300796-rmjv56ia	375	75	membrane	membrane	NN
cord-300796-rmjv56ia	375	76	protein	protein	NN
cord-300796-rmjv56ia	375	77	Evidence	evidence	NN
cord-300796-rmjv56ia	375	78	for	for	IN
cord-300796-rmjv56ia	375	79	the	the	DT
cord-300796-rmjv56ia	375	80	loop	loop	NN
cord-300796-rmjv56ia	375	81	model	model	NN
cord-300796-rmjv56ia	375	82	of	of	IN
cord-300796-rmjv56ia	375	83	signal	signal	NN
cord-300796-rmjv56ia	375	84	-	-	HYPH
cord-300796-rmjv56ia	375	85	sequence	sequence	NN
cord-300796-rmjv56ia	375	86	insertion	insertion	NN
cord-300796-rmjv56ia	375	87	into	into	IN
cord-300796-rmjv56ia	375	88	the	the	DT
cord-300796-rmjv56ia	375	89	endoplasmic	endoplasmic	NN
cord-300796-rmjv56ia	375	90	reticulum	reticulum	NN
cord-300796-rmjv56ia	375	91	On	on	IN
cord-300796-rmjv56ia	375	92	the	the	DT
cord-300796-rmjv56ia	375	93	translocation	translocation	NN
cord-300796-rmjv56ia	375	94	of	of	IN
cord-300796-rmjv56ia	375	95	proteins	protein	NNS
cord-300796-rmjv56ia	375	96	across	across	IN
cord-300796-rmjv56ia	375	97	membranes	membrane	NNS
cord-300796-rmjv56ia	375	98	On	on	IN
cord-300796-rmjv56ia	375	99	the	the	DT
cord-300796-rmjv56ia	375	100	transfer	transfer	NN
cord-300796-rmjv56ia	375	101	of	of	IN
cord-300796-rmjv56ia	375	102	integral	integral	JJ
cord-300796-rmjv56ia	375	103	proteins	protein	NNS
cord-300796-rmjv56ia	375	104	into	into	IN
cord-300796-rmjv56ia	375	105	membranes	membrane	NNS
cord-300796-rmjv56ia	375	106	Nascent	nascent	JJ
cord-300796-rmjv56ia	375	107	peptide	peptide	NN
cord-300796-rmjv56ia	375	108	as	as	IN
cord-300796-rmjv56ia	375	109	sole	sole	JJ
cord-300796-rmjv56ia	375	110	attachment	attachment	NN
cord-300796-rmjv56ia	375	111	of	of	IN
cord-300796-rmjv56ia	375	112	polysomes	polysome	NNS
cord-300796-rmjv56ia	375	113	to	to	IN
cord-300796-rmjv56ia	375	114	membranes	membrane	NNS
cord-300796-rmjv56ia	375	115	in	in	IN
cord-300796-rmjv56ia	375	116	bacteria	bacteria	NNS
cord-300796-rmjv56ia	376	1	An	an	DT
cord-300796-rmjv56ia	376	2	internal	internal	JJ
cord-300796-rmjv56ia	376	3	signal	signal	NN
cord-300796-rmjv56ia	376	4	sequence	sequence	NN
cord-300796-rmjv56ia	376	5	:	:	:
cord-300796-rmjv56ia	376	6	the	the	DT
cord-300796-rmjv56ia	376	7	asialoglycoprotein	asialoglycoprotein	NNP
cord-300796-rmjv56ia	376	8	receptor	receptor	NN
cord-300796-rmjv56ia	376	9	membrane	membrane	NN
cord-300796-rmjv56ia	376	10	anchor	anchor	NN
cord-300796-rmjv56ia	376	11	Complete	complete	JJ
cord-300796-rmjv56ia	376	12	nucleotide	nucleotide	JJ
cord-300796-rmjv56ia	376	13	sequence	sequence	NN
cord-300796-rmjv56ia	376	14	of	of	IN
cord-300796-rmjv56ia	376	15	the	the	DT
cord-300796-rmjv56ia	376	16	Escherichia	Escherichia	NNP
cord-300796-rmjv56ia	376	17	coli	coli	NNS
cord-300796-rmjv56ia	376	18	plasmid	plasmid	NN
cord-300796-rmjv56ia	376	19	pBR	pBR	VBN
cord-300796-rmjv56ia	376	20	322	322	CD
cord-300796-rmjv56ia	376	21	Subcellular	Subcellular	NNP
cord-300796-rmjv56ia	376	22	location	location	NN
cord-300796-rmjv56ia	376	23	of	of	IN
cord-300796-rmjv56ia	376	24	enzymes	enzyme	NNS
cord-300796-rmjv56ia	376	25	involved	involve	VBN
cord-300796-rmjv56ia	376	26	in	in	IN
cord-300796-rmjv56ia	376	27	the	the	DT
cord-300796-rmjv56ia	376	28	N	n	NN
cord-300796-rmjv56ia	376	29	-	-	HYPH
cord-300796-rmjv56ia	376	30	glycosylation	glycosylation	NN
cord-300796-rmjv56ia	376	31	and	and	CC
cord-300796-rmjv56ia	376	32	processing	processing	NN
cord-300796-rmjv56ia	376	33	of	of	IN
cord-300796-rmjv56ia	376	34	asparagine	asparagine	NN
cord-300796-rmjv56ia	376	35	-	-	HYPH
cord-300796-rmjv56ia	376	36	linked	link	VBN
cord-300796-rmjv56ia	376	37	oligosaccbarides	oligosaccbaride	NNS
cord-300796-rmjv56ia	376	38	in	in	IN
cord-300796-rmjv56ia	376	39	Saccharomyces	Saccharomyces	NNP
cord-300796-rmjv56ia	376	40	cerevisiae	cerevisiae	VBZ
cord-300796-rmjv56ia	376	41	Structural	structural	JJ
cord-300796-rmjv56ia	376	42	and	and	CC
cord-300796-rmjv56ia	376	43	thermodynamic	thermodynamic	JJ
cord-300796-rmjv56ia	376	44	aspects	aspect	NNS
cord-300796-rmjv56ia	376	45	of	of	IN
cord-300796-rmjv56ia	376	46	the	the	DT
cord-300796-rmjv56ia	376	47	transfer	transfer	NN
cord-300796-rmjv56ia	376	48	of	of	IN
cord-300796-rmjv56ia	376	49	proteins	protein	NNS
cord-300796-rmjv56ia	376	50	into	into	IN
cord-300796-rmjv56ia	376	51	and	and	CC
cord-300796-rmjv56ia	376	52	across	across	IN
cord-300796-rmjv56ia	376	53	membranes	membrane	NNS
cord-300796-rmjv56ia	376	54	Trans	Trans	NNP
cord-300796-rmjv56ia	376	55	-	-	NN
cord-300796-rmjv56ia	376	56	membrane	membrane	NN
cord-300796-rmjv56ia	376	57	translocation	translocation	NN
cord-300796-rmjv56ia	376	58	of	of	IN
cord-300796-rmjv56ia	376	59	proteins	protein	NNS
cord-300796-rmjv56ia	376	60	:	:	:
cord-300796-rmjv56ia	376	61	the	the	DT
cord-300796-rmjv56ia	376	62	direct	direct	JJ
cord-300796-rmjv56ia	376	63	transfer	transfer	NN
cord-300796-rmjv56ia	376	64	model	model	NN
cord-300796-rmjv56ia	376	65	Insertion	insertion	NN
cord-300796-rmjv56ia	376	66	of	of	IN
cord-300796-rmjv56ia	376	67	a	a	DT
cord-300796-rmjv56ia	376	68	multispanning	multispanne	VBG
cord-300796-rmjv56ia	376	69	membrane	membrane	NN
cord-300796-rmjv56ia	376	70	protein	protein	NN
cord-300796-rmjv56ia	376	71	occurs	occur	VBZ
cord-300796-rmjv56ia	376	72	sequentially	sequentially	RB
cord-300796-rmjv56ia	376	73	and	and	CC
cord-300796-rmjv56ia	376	74	requires	require	VBZ
cord-300796-rmjv56ia	376	75	only	only	RB
cord-300796-rmjv56ia	376	76	one	one	CD
cord-300796-rmjv56ia	376	77	signal	signal	NN
cord-300796-rmjv56ia	376	78	sequence	sequence	NN
cord-300796-rmjv56ia	376	79	Multiple	multiple	JJ
cord-300796-rmjv56ia	376	80	mechanisms	mechanism	NNS
cord-300796-rmjv56ia	376	81	of	of	IN
cord-300796-rmjv56ia	376	82	protein	protein	NN
cord-300796-rmjv56ia	376	83	insertion	insertion	NN
cord-300796-rmjv56ia	376	84	into	into	IN
cord-300796-rmjv56ia	376	85	and	and	CC
cord-300796-rmjv56ia	376	86	across	across	IN
cord-300796-rmjv56ia	377	1	membranes	membrane	NNS
cord-300796-rmjv56ia	377	2	A	a	DT
cord-300796-rmjv56ia	377	3	signal	signal	NN
cord-300796-rmjv56ia	377	4	sequence	sequence	NN
cord-300796-rmjv56ia	377	5	receptor	receptor	NN
cord-300796-rmjv56ia	377	6	in	in	IN
cord-300796-rmjv56ia	377	7	the	the	DT
cord-300796-rmjv56ia	377	8	endoplasmic	endoplasmic	NN
cord-300796-rmjv56ia	377	9	reticulum	reticulum	NN
cord-300796-rmjv56ia	377	10	membrane	membrane	NN
cord-300796-rmjv56ia	377	11	Constraint	constraint	NN
cord-300796-rmjv56ia	377	12	of	of	IN
cord-300796-rmjv56ia	377	13	the	the	DT
cord-300796-rmjv56ia	377	14	translational	translational	JJ
cord-300796-rmjv56ia	377	15	diffusion	diffusion	NN
cord-300796-rmjv56ia	377	16	of	of	IN
cord-300796-rmjv56ia	377	17	a	a	DT
cord-300796-rmjv56ia	377	18	membrane	membrane	NN
cord-300796-rmjv56ia	377	19	glycoprotein	glycoprotein	NN
cord-300796-rmjv56ia	377	20	by	by	IN
cord-300796-rmjv56ia	377	21	its	-PRON-	PRP$
cord-300796-rmjv56ia	377	22	external	external	JJ
cord-300796-rmjv56ia	377	23	domains	domain	NNS
cord-300796-rmjv56ia	378	1	The	the	DT
cord-300796-rmjv56ia	378	2	transmembrahe	transmembrahe	NN
cord-300796-rmjv56ia	378	3	segment	segment	NN
cord-300796-rmjv56ia	378	4	of	of	IN
cord-300796-rmjv56ia	378	5	the	the	DT
cord-300796-rmjv56ia	378	6	human	human	JJ
cord-300796-rmjv56ia	378	7	transferrin	transferrin	NN
cord-300796-rmjv56ia	378	8	receptor	receptor	NN
cord-300796-rmjv56ia	378	9	functions	function	NNS
cord-300796-rmjv56ia	378	10	as	as	IN
cord-300796-rmjv56ia	378	11	a	a	DT
cord-300796-rmjv56ia	378	12	signal	signal	NN
cord-300796-rmjv56ia	378	13	peptide	peptide	NN
cord-300796-rmjv56ia	378	14	We	-PRON-	PRP
cord-300796-rmjv56ia	378	15	thank	thank	VBP
cord-300796-rmjv56ia	378	16	Ernst	Ernst	NNP
cord-300796-rmjv56ia	378	17	Bause	Bause	NNP
cord-300796-rmjv56ia	378	18	for	for	IN
cord-300796-rmjv56ia	378	19	constructive	constructive	JJ
cord-300796-rmjv56ia	378	20	discussion	discussion	NN
cord-300796-rmjv56ia	378	21	;	;	:
cord-300796-rmjv56ia	378	22	Gunnar	Gunnar	NNP
cord-300796-rmjv56ia	378	23	von	von	NNP
cord-300796-rmjv56ia	378	24	Heijne	Heijne	NNP
cord-300796-rmjv56ia	378	25	and	and	CC
cord-300796-rmjv56ia	378	26	Michael	Michael	NNP
cord-300796-rmjv56ia	378	27	Baron	Baron	NNP
cord-300796-rmjv56ia	378	28	for	for	IN
cord-300796-rmjv56ia	378	29	critical	critical	JJ
cord-300796-rmjv56ia	378	30	reading	reading	NN
cord-300796-rmjv56ia	378	31	of	of	IN
cord-300796-rmjv56ia	378	32	the	the	DT
cord-300796-rmjv56ia	378	33	manuscript	manuscript	NN
cord-300796-rmjv56ia	378	34	;	;	:
cord-300796-rmjv56ia	378	35	Johanna	Johanna	NNP
cord-300796-rmjv56ia	378	36	Wahlberg	Wahlberg	NNP
cord-300796-rmjv56ia	378	37	for	for	IN
cord-300796-rmjv56ia	378	38	help	help	NN
cord-300796-rmjv56ia	378	39	with	with	IN
cord-300796-rmjv56ia	378	40	the	the	DT
cord-300796-rmjv56ia	378	41	figures	figure	NNS
cord-300796-rmjv56ia	378	42	;	;	:
cord-300796-rmjv56ia	378	43	Margareta	Margareta	NNP
cord-300796-rmjv56ia	378	44	Berg	Berg	NNP
cord-300796-rmjv56ia	378	45	,	,	,
cord-300796-rmjv56ia	378	46	Tuula	Tuula	NNP
cord-300796-rmjv56ia	378	47	Marminen	Marminen	NNP
cord-300796-rmjv56ia	378	48	,	,	,
cord-300796-rmjv56ia	378	49	and	and	CC
cord-300796-rmjv56ia	378	50	Elisabeth	Elisabeth	NNP
cord-300796-rmjv56ia	378	51	Servin	Servin	NNP
cord-300796-rmjv56ia	378	52	for	for	IN
cord-300796-rmjv56ia	378	53	technical	technical	JJ
cord-300796-rmjv56ia	378	54	assistance	assistance	NN
cord-300796-rmjv56ia	378	55	;	;	,
cord-300796-rmjv56ia	378	56	and	and	CC
cord-300796-rmjv56ia	378	57	Ingrid	Ingrid	NNP
cord-300796-rmjv56ia	378	58	Sigurdson	Sigurdson	NNP
cord-300796-rmjv56ia	378	59	for	for	IN
cord-300796-rmjv56ia	378	60	typing	typing	NN
cord-300796-rmjv56ia	378	61	.	.	.
cord-300796-rmjv56ia	379	1	This	this	DT
cord-300796-rmjv56ia	379	2	work	work	NN
cord-300796-rmjv56ia	379	3	was	be	VBD
cord-300796-rmjv56ia	379	4	supported	support	VBN
cord-300796-rmjv56ia	379	5	by	by	IN
cord-300796-rmjv56ia	379	6	grants	grant	NNS
cord-300796-rmjv56ia	379	7	from	from	IN
cord-300796-rmjv56ia	379	8	the	the	DT
cord-300796-rmjv56ia	379	9	Swedish	Swedish	NNP
cord-300796-rmjv56ia	379	10	Medical	Medical	NNP
cord-300796-rmjv56ia	379	11	Research	Research	NNP
cord-300796-rmjv56ia	379	12	Council	Council	NNP
cord-300796-rmjv56ia	379	13	(	(	-LRB-
cord-300796-rmjv56ia	379	14	B88	B88	NNP
cord-300796-rmjv56ia	379	15	-	-	HYPH
cord-300796-rmjv56ia	379	16	12X-08272	12X-08272	NNP
cord-300796-rmjv56ia	379	17	-	-	HYPH
cord-300796-rmjv56ia	379	18	01A	01a	CD
cord-300796-rmjv56ia	379	19	)	)	-RRB-
cord-300796-rmjv56ia	379	20	,	,	,
cord-300796-rmjv56ia	379	21	Swedish	Swedish	NNP
cord-300796-rmjv56ia	379	22	Natural	Natural	NNP
cord-300796-rmjv56ia	379	23	Science	Science	NNP
cord-300796-rmjv56ia	379	24	Research	Research	NNP
cord-300796-rmjv56ia	379	25	Council	Council	NNP
cord-300796-rmjv56ia	379	26	(	(	-LRB-
cord-300796-rmjv56ia	379	27	B	B	NNP
cord-300796-rmjv56ia	379	28	-	-	HYPH
cord-300796-rmjv56ia	379	29	BU	BU	NNP
cord-300796-rmjv56ia	379	30	9353	9353	CD
cord-300796-rmjv56ia	379	31	-	-	HYPH
cord-300796-rmjv56ia	379	32	300	300	CD
cord-300796-rmjv56ia	379	33	)	)	-RRB-
cord-300796-rmjv56ia	379	34	,	,	,
cord-300796-rmjv56ia	379	35	and	and	CC
cord-300796-rmjv56ia	379	36	Swedish	Swedish	NNP
cord-300796-rmjv56ia	379	37	National	National	NNP
cord-300796-rmjv56ia	379	38	Board	Board	NNP
cord-300796-rmjv56ia	379	39	for	for	IN
cord-300796-rmjv56ia	379	40	Technical	Technical	NNP
cord-300796-rmjv56ia	379	41	Development	Development	NNP
cord-300796-rmjv56ia	379	42	(	(	-LRB-
cord-300796-rmjv56ia	379	43	87	87	CD
cord-300796-rmjv56ia	379	44	-	-	HYPH
cord-300796-rmjv56ia	379	45	02750P).Received	02750p).receive	VBN
cord-300796-rmjv56ia	379	46	for	for	IN
cord-300796-rmjv56ia	379	47	publication	publication	NN
cord-300796-rmjv56ia	379	48	7	7	CD
cord-300796-rmjv56ia	379	49	March	March	NNP
cord-300796-rmjv56ia	379	50	1990	1990	CD
cord-300796-rmjv56ia	379	51	and	and	CC
cord-300796-rmjv56ia	379	52	in	in	IN
cord-300796-rmjv56ia	379	53	revised	revise	VBN
cord-300796-rmjv56ia	379	54	form	form	NN
cord-300796-rmjv56ia	379	55	2	2	CD
cord-300796-rmjv56ia	379	56	May	May	NNP
cord-300796-rmjv56ia	379	57	1990	1990	CD
cord-300796-rmjv56ia	379	58	.	.	.