id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-321868-xk4yuibj	Belcourt, Michael F.	Ribosomal frameshifting in the yeast retrotransposon Ty: tRNAs induce slippage on a 7 nucleotide minimal site	1990-07-27		.txt	text/plain	9563	556	65	Our tRNA overproduction data suggest that a leucyl-tRNA, probably tRNALeu UAG, an unusual leucine isoacceptor that recognizes all six leucine codons, slips from CUU-Leu onto UUA-Leu (in the +1 reading frame) during a translational pause at the AGG-Arg codon induced by the low availability of tRNAArg CCU, encoded by a single-copy essential gene. In these systems, a simultaneous slippage of tRNAs in the A and P sites of the translating ribosome on the homopolymeric sequence results in a frameshift to the pro orpol reading frame and suppression of the gag frame termination codon. The 14 nucleotide frameshift site has a Gln residue (rather than His) following Gly. The amino acid sequence through the 14 nucleotide site confirms the Gln residue after Gly (data not shown), supporting the notion that translation shifts to the +l reading frame by peptidyl-tRNA slippage on the CUU-Leu codon.	./cache/cord-321868-xk4yuibj.txt	./txt/cord-321868-xk4yuibj.txt