id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_j3q6nxsyw5brjdm2bza4ksnwj4	Weifeng Zhao	A Simple and Efficient Method for Isolation of Pineapple Protoplasts	2011	4	.pdf	application/pdf	1787	185	59	Keywords: pineapple, protoplast, isolation, enzyme preliminarily studied the protoplast isolation of pineapple by order to develop a simple and high-yield method for protoplast isolation of pineapple and then could be used to protoplast macerozyme R-10, 1.5% cellulase R-10 and 5% mannitol solution, pH 5.5, followed by differential and gradient centrifugations. filtering the resulting enzyme-protoplast mixture through a 425 was resuspended in 5 ml cPW 27S solution (cell-protoplast osmotic pressure of enzyme mixture, cPW washing solution different concentration gradients of mannitol and sucrose. effects of different enzyme combinations and treatment time on effects of different enzyme combinations and treatment time on result showed that their differences in protoplast yield are enzyme combination i and ii, high protoplast yield required low protoplast viability using high concentration of enzyme protoplast viability (88.9%) was obtained when using enzyme protoplast yield and viability would be obtained when using After filtering and centrifuging enzyme-protoplast mixture, 	./cache/work_j3q6nxsyw5brjdm2bza4ksnwj4.pdf	./txt/work_j3q6nxsyw5brjdm2bza4ksnwj4.txt