id sid tid token lemma pos 10_1101-2021_01_07_425794 1 1 Impact impact NN 10_1101-2021_01_07_425794 1 2 of of IN 10_1101-2021_01_07_425794 1 3 gene gene NN 10_1101-2021_01_07_425794 1 4 annotation annotation NN 10_1101-2021_01_07_425794 1 5 choice choice NN 10_1101-2021_01_07_425794 1 6 on on IN 10_1101-2021_01_07_425794 1 7 the the DT 10_1101-2021_01_07_425794 1 8 quantification quantification NN 10_1101-2021_01_07_425794 1 9 of of IN 10_1101-2021_01_07_425794 1 10 RNA RNA NNP 10_1101-2021_01_07_425794 1 11 - - HYPH 10_1101-2021_01_07_425794 1 12 seq seq NN 10_1101-2021_01_07_425794 1 13 data datum NNS 10_1101-2021_01_07_425794 1 14 Impact impact NN 10_1101-2021_01_07_425794 1 15 of of IN 10_1101-2021_01_07_425794 1 16 gene gene NN 10_1101-2021_01_07_425794 1 17 annotation annotation NN 10_1101-2021_01_07_425794 1 18 choice choice NN 10_1101-2021_01_07_425794 1 19 on on IN 10_1101-2021_01_07_425794 1 20 the the DT 10_1101-2021_01_07_425794 1 21 quantification quantification NN 10_1101-2021_01_07_425794 1 22 of of IN 10_1101-2021_01_07_425794 1 23 RNA RNA NNP 10_1101-2021_01_07_425794 1 24 - - HYPH 10_1101-2021_01_07_425794 1 25 seq seq NN 10_1101-2021_01_07_425794 1 26 data datum NNS 10_1101-2021_01_07_425794 1 27 David David NNP 10_1101-2021_01_07_425794 1 28 Chisanga Chisanga NNP 10_1101-2021_01_07_425794 1 29 1,2,3,4 1,2,3,4 NN 10_1101-2021_01_07_425794 1 30 , , , 10_1101-2021_01_07_425794 1 31 Yang Yang NNP 10_1101-2021_01_07_425794 1 32 Liao Liao NNP 10_1101-2021_01_07_425794 1 33 1,2,3,4 1,2,3,4 NN 10_1101-2021_01_07_425794 1 34 and and CC 10_1101-2021_01_07_425794 1 35 Wei Wei NNP 10_1101-2021_01_07_425794 1 36 Shi Shi NNP 10_1101-2021_01_07_425794 1 37 1,2,3,5 1,2,3,5 NN 10_1101-2021_01_07_425794 1 38 * * NFP 10_1101-2021_01_07_425794 1 39 1Olivia 1Olivia NNP 10_1101-2021_01_07_425794 1 40 Newton Newton NNP 10_1101-2021_01_07_425794 1 41 - - HYPH 10_1101-2021_01_07_425794 1 42 John John NNP 10_1101-2021_01_07_425794 1 43 Cancer Cancer NNP 10_1101-2021_01_07_425794 1 44 Research Research NNP 10_1101-2021_01_07_425794 1 45 Institute Institute NNP 10_1101-2021_01_07_425794 1 46 , , , 10_1101-2021_01_07_425794 1 47 Heidelberg Heidelberg NNP 10_1101-2021_01_07_425794 1 48 , , , 10_1101-2021_01_07_425794 1 49 Victoria Victoria NNP 10_1101-2021_01_07_425794 1 50 , , , 10_1101-2021_01_07_425794 1 51 3084 3084 CD 10_1101-2021_01_07_425794 1 52 , , , 10_1101-2021_01_07_425794 1 53 Australia Australia NNP 10_1101-2021_01_07_425794 1 54 , , , 10_1101-2021_01_07_425794 1 55 2School 2School NNS 10_1101-2021_01_07_425794 1 56 of of IN 10_1101-2021_01_07_425794 1 57 Cancer Cancer NNP 10_1101-2021_01_07_425794 1 58 Medicine Medicine NNP 10_1101-2021_01_07_425794 1 59 , , , 10_1101-2021_01_07_425794 1 60 La La NNP 10_1101-2021_01_07_425794 1 61 Trobe Trobe NNP 10_1101-2021_01_07_425794 1 62 University University NNP 10_1101-2021_01_07_425794 1 63 , , , 10_1101-2021_01_07_425794 1 64 Bundoora Bundoora NNP 10_1101-2021_01_07_425794 1 65 , , , 10_1101-2021_01_07_425794 1 66 Victoria Victoria NNP 10_1101-2021_01_07_425794 1 67 , , , 10_1101-2021_01_07_425794 1 68 3083 3083 CD 10_1101-2021_01_07_425794 1 69 , , , 10_1101-2021_01_07_425794 1 70 Australia Australia NNP 10_1101-2021_01_07_425794 1 71 , , , 10_1101-2021_01_07_425794 1 72 3Walter 3Walter NNP 10_1101-2021_01_07_425794 1 73 and and CC 10_1101-2021_01_07_425794 1 74 Eliza Eliza NNP 10_1101-2021_01_07_425794 1 75 Hall Hall NNP 10_1101-2021_01_07_425794 1 76 Institute Institute NNP 10_1101-2021_01_07_425794 1 77 of of IN 10_1101-2021_01_07_425794 1 78 Medical Medical NNP 10_1101-2021_01_07_425794 1 79 Research Research NNP 10_1101-2021_01_07_425794 1 80 , , , 10_1101-2021_01_07_425794 1 81 Parkville Parkville NNP 10_1101-2021_01_07_425794 1 82 , , , 10_1101-2021_01_07_425794 1 83 Victoria Victoria NNP 10_1101-2021_01_07_425794 1 84 , , , 10_1101-2021_01_07_425794 1 85 3052 3052 CD 10_1101-2021_01_07_425794 1 86 , , , 10_1101-2021_01_07_425794 1 87 Australia Australia NNP 10_1101-2021_01_07_425794 1 88 , , , 10_1101-2021_01_07_425794 1 89 4Department 4department NN 10_1101-2021_01_07_425794 1 90 of of IN 10_1101-2021_01_07_425794 1 91 Medical Medical NNP 10_1101-2021_01_07_425794 1 92 Biology Biology NNP 10_1101-2021_01_07_425794 1 93 , , , 10_1101-2021_01_07_425794 1 94 The the DT 10_1101-2021_01_07_425794 1 95 University University NNP 10_1101-2021_01_07_425794 1 96 of of IN 10_1101-2021_01_07_425794 1 97 Melbourne Melbourne NNP 10_1101-2021_01_07_425794 1 98 , , , 10_1101-2021_01_07_425794 1 99 Parkville Parkville NNP 10_1101-2021_01_07_425794 1 100 , , , 10_1101-2021_01_07_425794 1 101 Victoria Victoria NNP 10_1101-2021_01_07_425794 1 102 , , , 10_1101-2021_01_07_425794 1 103 3010 3010 CD 10_1101-2021_01_07_425794 1 104 , , , 10_1101-2021_01_07_425794 1 105 Australia Australia NNP 10_1101-2021_01_07_425794 1 106 and and CC 10_1101-2021_01_07_425794 1 107 5School 5School NNP 10_1101-2021_01_07_425794 1 108 of of IN 10_1101-2021_01_07_425794 1 109 Computing Computing NNP 10_1101-2021_01_07_425794 1 110 and and CC 10_1101-2021_01_07_425794 1 111 Information Information NNP 10_1101-2021_01_07_425794 1 112 Systems Systems NNPS 10_1101-2021_01_07_425794 1 113 , , , 10_1101-2021_01_07_425794 1 114 The the DT 10_1101-2021_01_07_425794 1 115 University University NNP 10_1101-2021_01_07_425794 1 116 of of IN 10_1101-2021_01_07_425794 1 117 Mel- Mel- NNP 10_1101-2021_01_07_425794 1 118 bourne bourne NNP 10_1101-2021_01_07_425794 1 119 , , , 10_1101-2021_01_07_425794 1 120 Parkville Parkville NNP 10_1101-2021_01_07_425794 1 121 , , , 10_1101-2021_01_07_425794 1 122 Victoria Victoria NNP 10_1101-2021_01_07_425794 1 123 , , , 10_1101-2021_01_07_425794 1 124 3010 3010 CD 10_1101-2021_01_07_425794 1 125 , , , 10_1101-2021_01_07_425794 1 126 Australia Australia NNP 10_1101-2021_01_07_425794 1 127 Abstract Abstract NNP 10_1101-2021_01_07_425794 1 128 RNA RNA NNP 10_1101-2021_01_07_425794 1 129 sequencing sequencing NN 10_1101-2021_01_07_425794 1 130 is be VBZ 10_1101-2021_01_07_425794 1 131 currently currently RB 10_1101-2021_01_07_425794 1 132 the the DT 10_1101-2021_01_07_425794 1 133 method method NN 10_1101-2021_01_07_425794 1 134 of of IN 10_1101-2021_01_07_425794 1 135 choice choice NN 10_1101-2021_01_07_425794 1 136 for for IN 10_1101-2021_01_07_425794 1 137 genome genome NN 10_1101-2021_01_07_425794 1 138 - - HYPH 10_1101-2021_01_07_425794 1 139 wide wide JJ 10_1101-2021_01_07_425794 1 140 profiling profiling NN 10_1101-2021_01_07_425794 1 141 of of IN 10_1101-2021_01_07_425794 1 142 gene gene NN 10_1101-2021_01_07_425794 1 143 expression expression NN 10_1101-2021_01_07_425794 1 144 . . . 10_1101-2021_01_07_425794 2 1 A a DT 10_1101-2021_01_07_425794 2 2 popular popular JJ 10_1101-2021_01_07_425794 2 3 approach approach NN 10_1101-2021_01_07_425794 2 4 to to TO 10_1101-2021_01_07_425794 2 5 quantify quantify VB 10_1101-2021_01_07_425794 2 6 expression expression NN 10_1101-2021_01_07_425794 2 7 levels level NNS 10_1101-2021_01_07_425794 2 8 of of IN 10_1101-2021_01_07_425794 2 9 genes gene NNS 10_1101-2021_01_07_425794 2 10 from from IN 10_1101-2021_01_07_425794 2 11 RNA RNA NNP 10_1101-2021_01_07_425794 2 12 - - HYPH 10_1101-2021_01_07_425794 2 13 seq seq NN 10_1101-2021_01_07_425794 2 14 data datum NNS 10_1101-2021_01_07_425794 2 15 is be VBZ 10_1101-2021_01_07_425794 2 16 to to TO 10_1101-2021_01_07_425794 2 17 map map VB 10_1101-2021_01_07_425794 2 18 reads read VBZ 10_1101-2021_01_07_425794 2 19 to to IN 10_1101-2021_01_07_425794 2 20 a a DT 10_1101-2021_01_07_425794 2 21 reference reference NN 10_1101-2021_01_07_425794 2 22 genome genome JJ 10_1101-2021_01_07_425794 2 23 and and CC 10_1101-2021_01_07_425794 2 24 then then RB 10_1101-2021_01_07_425794 2 25 count count VB 10_1101-2021_01_07_425794 2 26 mapped map VBD 10_1101-2021_01_07_425794 2 27 reads read VBZ 10_1101-2021_01_07_425794 2 28 to to IN 10_1101-2021_01_07_425794 2 29 each each DT 10_1101-2021_01_07_425794 2 30 gene gene NN 10_1101-2021_01_07_425794 2 31 . . . 10_1101-2021_01_07_425794 3 1 Gene gene NN 10_1101-2021_01_07_425794 3 2 annotation annotation NN 10_1101-2021_01_07_425794 3 3 data datum NNS 10_1101-2021_01_07_425794 3 4 , , , 10_1101-2021_01_07_425794 3 5 which which WDT 10_1101-2021_01_07_425794 3 6 include include VBP 10_1101-2021_01_07_425794 3 7 chromosomal chromosomal JJ 10_1101-2021_01_07_425794 3 8 coordinates coordinate NNS 10_1101-2021_01_07_425794 3 9 of of IN 10_1101-2021_01_07_425794 3 10 exons exon NNS 10_1101-2021_01_07_425794 3 11 for for IN 10_1101-2021_01_07_425794 3 12 tens ten NNS 10_1101-2021_01_07_425794 3 13 of of IN 10_1101-2021_01_07_425794 3 14 thousands thousand NNS 10_1101-2021_01_07_425794 3 15 of of IN 10_1101-2021_01_07_425794 3 16 genes gene NNS 10_1101-2021_01_07_425794 3 17 , , , 10_1101-2021_01_07_425794 3 18 are be VBP 10_1101-2021_01_07_425794 3 19 required require VBN 10_1101-2021_01_07_425794 3 20 for for IN 10_1101-2021_01_07_425794 3 21 this this DT 10_1101-2021_01_07_425794 3 22 quantification quantification NN 10_1101-2021_01_07_425794 3 23 process process NN 10_1101-2021_01_07_425794 3 24 . . . 10_1101-2021_01_07_425794 4 1 There there EX 10_1101-2021_01_07_425794 4 2 are be VBP 10_1101-2021_01_07_425794 4 3 several several JJ 10_1101-2021_01_07_425794 4 4 major major JJ 10_1101-2021_01_07_425794 4 5 sources source NNS 10_1101-2021_01_07_425794 4 6 of of IN 10_1101-2021_01_07_425794 4 7 gene gene NN 10_1101-2021_01_07_425794 4 8 annotations annotation NNS 10_1101-2021_01_07_425794 4 9 that that WDT 10_1101-2021_01_07_425794 4 10 can can MD 10_1101-2021_01_07_425794 4 11 be be VB 10_1101-2021_01_07_425794 4 12 used use VBN 10_1101-2021_01_07_425794 4 13 for for IN 10_1101-2021_01_07_425794 4 14 quantification quantification NN 10_1101-2021_01_07_425794 4 15 , , , 10_1101-2021_01_07_425794 4 16 such such JJ 10_1101-2021_01_07_425794 4 17 as as IN 10_1101-2021_01_07_425794 4 18 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 4 19 and and CC 10_1101-2021_01_07_425794 4 20 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 4 21 databases database NNS 10_1101-2021_01_07_425794 4 22 . . . 10_1101-2021_01_07_425794 5 1 However however RB 10_1101-2021_01_07_425794 5 2 , , , 10_1101-2021_01_07_425794 5 3 there there EX 10_1101-2021_01_07_425794 5 4 is be VBZ 10_1101-2021_01_07_425794 5 5 very very RB 10_1101-2021_01_07_425794 5 6 little little JJ 10_1101-2021_01_07_425794 5 7 understanding understanding NN 10_1101-2021_01_07_425794 5 8 of of IN 10_1101-2021_01_07_425794 5 9 the the DT 10_1101-2021_01_07_425794 5 10 effect effect NN 10_1101-2021_01_07_425794 5 11 that that WDT 10_1101-2021_01_07_425794 5 12 the the DT 10_1101-2021_01_07_425794 5 13 choice choice NN 10_1101-2021_01_07_425794 5 14 of of IN 10_1101-2021_01_07_425794 5 15 annotation annotation NN 10_1101-2021_01_07_425794 5 16 has have VBZ 10_1101-2021_01_07_425794 5 17 on on IN 10_1101-2021_01_07_425794 5 18 the the DT 10_1101-2021_01_07_425794 5 19 accuracy accuracy NN 10_1101-2021_01_07_425794 5 20 of of IN 10_1101-2021_01_07_425794 5 21 gene gene NN 10_1101-2021_01_07_425794 5 22 expression expression NN 10_1101-2021_01_07_425794 5 23 quantification quantification NN 10_1101-2021_01_07_425794 5 24 in in IN 10_1101-2021_01_07_425794 5 25 an an DT 10_1101-2021_01_07_425794 5 26 RNA RNA NNP 10_1101-2021_01_07_425794 5 27 - - HYPH 10_1101-2021_01_07_425794 5 28 seq seq NN 10_1101-2021_01_07_425794 5 29 analysis analysis NN 10_1101-2021_01_07_425794 5 30 . . . 10_1101-2021_01_07_425794 6 1 In in IN 10_1101-2021_01_07_425794 6 2 this this DT 10_1101-2021_01_07_425794 6 3 paper paper NN 10_1101-2021_01_07_425794 6 4 , , , 10_1101-2021_01_07_425794 6 5 we -PRON- PRP 10_1101-2021_01_07_425794 6 6 present present VBP 10_1101-2021_01_07_425794 6 7 results result NNS 10_1101-2021_01_07_425794 6 8 from from IN 10_1101-2021_01_07_425794 6 9 our -PRON- PRP$ 10_1101-2021_01_07_425794 6 10 comparison comparison NN 10_1101-2021_01_07_425794 6 11 of of IN 10_1101-2021_01_07_425794 6 12 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 6 13 and and CC 10_1101-2021_01_07_425794 6 14 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 6 15 human human JJ 10_1101-2021_01_07_425794 6 16 annotations annotation NNS 10_1101-2021_01_07_425794 6 17 on on IN 10_1101-2021_01_07_425794 6 18 their -PRON- PRP$ 10_1101-2021_01_07_425794 6 19 impact impact NN 10_1101-2021_01_07_425794 6 20 on on IN 10_1101-2021_01_07_425794 6 21 gene gene NN 10_1101-2021_01_07_425794 6 22 expression expression NN 10_1101-2021_01_07_425794 6 23 quantification quantification NN 10_1101-2021_01_07_425794 6 24 using use VBG 10_1101-2021_01_07_425794 6 25 a a DT 10_1101-2021_01_07_425794 6 26 benchmark benchmark JJ 10_1101-2021_01_07_425794 6 27 RNA RNA NNP 10_1101-2021_01_07_425794 6 28 - - HYPH 10_1101-2021_01_07_425794 6 29 seq seq NN 10_1101-2021_01_07_425794 6 30 dataset dataset NN 10_1101-2021_01_07_425794 6 31 generated generate VBN 10_1101-2021_01_07_425794 6 32 by by IN 10_1101-2021_01_07_425794 6 33 the the DT 10_1101-2021_01_07_425794 6 34 SEquencing SEquencing NNP 10_1101-2021_01_07_425794 6 35 Quality Quality NNP 10_1101-2021_01_07_425794 6 36 Control Control NNP 10_1101-2021_01_07_425794 6 37 ( ( -LRB- 10_1101-2021_01_07_425794 6 38 SEQC SEQC NNP 10_1101-2021_01_07_425794 6 39 ) ) -RRB- 10_1101-2021_01_07_425794 6 40 consortium consortium NN 10_1101-2021_01_07_425794 6 41 . . . 10_1101-2021_01_07_425794 7 1 We -PRON- PRP 10_1101-2021_01_07_425794 7 2 show show VBP 10_1101-2021_01_07_425794 7 3 that that IN 10_1101-2021_01_07_425794 7 4 the the DT 10_1101-2021_01_07_425794 7 5 use use NN 10_1101-2021_01_07_425794 7 6 of of IN 10_1101-2021_01_07_425794 7 7 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 7 8 gene gene NN 10_1101-2021_01_07_425794 7 9 annotation annotation NN 10_1101-2021_01_07_425794 7 10 models model NNS 10_1101-2021_01_07_425794 7 11 led lead VBN 10_1101-2021_01_07_425794 7 12 to to IN 10_1101-2021_01_07_425794 7 13 better well JJR 10_1101-2021_01_07_425794 7 14 quantification quantification NN 10_1101-2021_01_07_425794 7 15 accuracy accuracy NN 10_1101-2021_01_07_425794 7 16 , , , 10_1101-2021_01_07_425794 7 17 based base VBN 10_1101-2021_01_07_425794 7 18 on on IN 10_1101-2021_01_07_425794 7 19 the the DT 10_1101-2021_01_07_425794 7 20 correlation correlation NN 10_1101-2021_01_07_425794 7 21 with with IN 10_1101-2021_01_07_425794 7 22 ground ground NN 10_1101-2021_01_07_425794 7 23 truths truth NNS 10_1101-2021_01_07_425794 7 24 including include VBG 10_1101-2021_01_07_425794 7 25 expression expression NN 10_1101-2021_01_07_425794 7 26 data datum NNS 10_1101-2021_01_07_425794 7 27 from from IN 10_1101-2021_01_07_425794 7 28 > > XX 10_1101-2021_01_07_425794 7 29 800 800 CD 10_1101-2021_01_07_425794 7 30 real real JJ 10_1101-2021_01_07_425794 7 31 - - HYPH 10_1101-2021_01_07_425794 7 32 time time NN 10_1101-2021_01_07_425794 7 33 PCR PCR NNP 10_1101-2021_01_07_425794 7 34 validated validate VBN 10_1101-2021_01_07_425794 7 35 genes gene NNS 10_1101-2021_01_07_425794 7 36 , , , 10_1101-2021_01_07_425794 7 37 known know VBN 10_1101-2021_01_07_425794 7 38 titration titration NN 10_1101-2021_01_07_425794 7 39 ratios ratio NNS 10_1101-2021_01_07_425794 7 40 of of IN 10_1101-2021_01_07_425794 7 41 gene gene NN 10_1101-2021_01_07_425794 7 42 expression expression NN 10_1101-2021_01_07_425794 7 43 and and CC 10_1101-2021_01_07_425794 7 44 microarray microarray JJ 10_1101-2021_01_07_425794 7 45 expression expression NN 10_1101-2021_01_07_425794 7 46 data datum NNS 10_1101-2021_01_07_425794 7 47 . . . 10_1101-2021_01_07_425794 8 1 We -PRON- PRP 10_1101-2021_01_07_425794 8 2 also also RB 10_1101-2021_01_07_425794 8 3 found find VBD 10_1101-2021_01_07_425794 8 4 that that IN 10_1101-2021_01_07_425794 8 5 the the DT 10_1101-2021_01_07_425794 8 6 recent recent JJ 10_1101-2021_01_07_425794 8 7 expansion expansion NN 10_1101-2021_01_07_425794 8 8 of of IN 10_1101-2021_01_07_425794 8 9 the the DT 10_1101-2021_01_07_425794 8 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 8 11 annotation annotation NN 10_1101-2021_01_07_425794 8 12 has have VBZ 10_1101-2021_01_07_425794 8 13 led lead VBN 10_1101-2021_01_07_425794 8 14 to to IN 10_1101-2021_01_07_425794 8 15 a a DT 10_1101-2021_01_07_425794 8 16 decrease decrease NN 10_1101-2021_01_07_425794 8 17 in in IN 10_1101-2021_01_07_425794 8 18 its -PRON- PRP$ 10_1101-2021_01_07_425794 8 19 annotation annotation NN 10_1101-2021_01_07_425794 8 20 accuracy accuracy NN 10_1101-2021_01_07_425794 8 21 . . . 10_1101-2021_01_07_425794 9 1 Finally finally RB 10_1101-2021_01_07_425794 9 2 , , , 10_1101-2021_01_07_425794 9 3 we -PRON- PRP 10_1101-2021_01_07_425794 9 4 demonstrated demonstrate VBD 10_1101-2021_01_07_425794 9 5 that that IN 10_1101-2021_01_07_425794 9 6 the the DT 10_1101-2021_01_07_425794 9 7 RNA RNA NNP 10_1101-2021_01_07_425794 9 8 - - HYPH 10_1101-2021_01_07_425794 9 9 seq seq NN 10_1101-2021_01_07_425794 9 10 quantification quantification NN 10_1101-2021_01_07_425794 9 11 differences difference NNS 10_1101-2021_01_07_425794 9 12 observed observe VBN 10_1101-2021_01_07_425794 9 13 between between IN 10_1101-2021_01_07_425794 9 14 different different JJ 10_1101-2021_01_07_425794 9 15 annotations annotation NNS 10_1101-2021_01_07_425794 9 16 were be VBD 10_1101-2021_01_07_425794 9 17 not not RB 10_1101-2021_01_07_425794 9 18 affected affect VBN 10_1101-2021_01_07_425794 9 19 by by IN 10_1101-2021_01_07_425794 9 20 the the DT 10_1101-2021_01_07_425794 9 21 use use NN 10_1101-2021_01_07_425794 9 22 of of IN 10_1101-2021_01_07_425794 9 23 different different JJ 10_1101-2021_01_07_425794 9 24 normalization normalization NN 10_1101-2021_01_07_425794 9 25 methods method NNS 10_1101-2021_01_07_425794 9 26 . . . 10_1101-2021_01_07_425794 10 1 * * NFP 10_1101-2021_01_07_425794 10 2 To to IN 10_1101-2021_01_07_425794 10 3 whom whom WP 10_1101-2021_01_07_425794 10 4 correspondence correspondence NN 10_1101-2021_01_07_425794 10 5 should should MD 10_1101-2021_01_07_425794 10 6 be be VB 10_1101-2021_01_07_425794 10 7 addressed address VBN 10_1101-2021_01_07_425794 10 8 . . . 10_1101-2021_01_07_425794 11 1 Tel tel NN 10_1101-2021_01_07_425794 11 2 : : : 10_1101-2021_01_07_425794 11 3 +61 +61 NNP 10_1101-2021_01_07_425794 11 4 3 3 CD 10_1101-2021_01_07_425794 11 5 9496 9496 CD 10_1101-2021_01_07_425794 11 6 5726 5726 CD 10_1101-2021_01_07_425794 11 7 ; ; : 10_1101-2021_01_07_425794 11 8 Fax fax NN 10_1101-2021_01_07_425794 11 9 : : : 10_1101-2021_01_07_425794 11 10 +61 +61 NNP 10_1101-2021_01_07_425794 11 11 3 3 CD 10_1101-2021_01_07_425794 11 12 9496 9496 CD 10_1101-2021_01_07_425794 11 13 5334 5334 CD 10_1101-2021_01_07_425794 11 14 ; ; : 10_1101-2021_01_07_425794 11 15 Email email NN 10_1101-2021_01_07_425794 11 16 : : : 10_1101-2021_01_07_425794 11 17 Wei.Shi@onjcri.org.au wei.shi@onjcri.org.au NN 10_1101-2021_01_07_425794 11 18 1 1 CD 10_1101-2021_01_07_425794 11 19 .CC .CC : 10_1101-2021_01_07_425794 11 20 - - HYPH 10_1101-2021_01_07_425794 11 21 BY by IN 10_1101-2021_01_07_425794 11 22 - - HYPH 10_1101-2021_01_07_425794 11 23 NC NC NNP 10_1101-2021_01_07_425794 11 24 - - HYPH 10_1101-2021_01_07_425794 11 25 ND ND NNP 10_1101-2021_01_07_425794 11 26 4.0 4.0 CD 10_1101-2021_01_07_425794 11 27 International international JJ 10_1101-2021_01_07_425794 11 28 licensemade licensemade NN 10_1101-2021_01_07_425794 11 29 available available JJ 10_1101-2021_01_07_425794 11 30 under under IN 10_1101-2021_01_07_425794 11 31 a a DT 10_1101-2021_01_07_425794 11 32 ( ( -LRB- 10_1101-2021_01_07_425794 11 33 which which WDT 10_1101-2021_01_07_425794 11 34 was be VBD 10_1101-2021_01_07_425794 11 35 not not RB 10_1101-2021_01_07_425794 11 36 certified certify VBN 10_1101-2021_01_07_425794 11 37 by by IN 10_1101-2021_01_07_425794 11 38 peer peer NN 10_1101-2021_01_07_425794 11 39 review review NN 10_1101-2021_01_07_425794 11 40 ) ) -RRB- 10_1101-2021_01_07_425794 11 41 is be VBZ 10_1101-2021_01_07_425794 11 42 the the DT 10_1101-2021_01_07_425794 11 43 author author NN 10_1101-2021_01_07_425794 11 44 / / SYM 10_1101-2021_01_07_425794 11 45 funder funder NN 10_1101-2021_01_07_425794 11 46 , , , 10_1101-2021_01_07_425794 11 47 who who WP 10_1101-2021_01_07_425794 11 48 has have VBZ 10_1101-2021_01_07_425794 11 49 granted grant VBN 10_1101-2021_01_07_425794 11 50 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 11 51 a a DT 10_1101-2021_01_07_425794 11 52 license license NN 10_1101-2021_01_07_425794 11 53 to to TO 10_1101-2021_01_07_425794 11 54 display display VB 10_1101-2021_01_07_425794 11 55 the the DT 10_1101-2021_01_07_425794 11 56 preprint preprint NN 10_1101-2021_01_07_425794 11 57 in in IN 10_1101-2021_01_07_425794 11 58 perpetuity perpetuity NN 10_1101-2021_01_07_425794 11 59 . . . 10_1101-2021_01_07_425794 12 1 It -PRON- PRP 10_1101-2021_01_07_425794 12 2 is be VBZ 10_1101-2021_01_07_425794 12 3 The the DT 10_1101-2021_01_07_425794 12 4 copyright copyright NN 10_1101-2021_01_07_425794 12 5 holder holder NN 10_1101-2021_01_07_425794 12 6 for for IN 10_1101-2021_01_07_425794 12 7 this this DT 10_1101-2021_01_07_425794 12 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 12 9 version version NN 10_1101-2021_01_07_425794 12 10 posted post VBD 10_1101-2021_01_07_425794 12 11 January January NNP 10_1101-2021_01_07_425794 12 12 8 8 CD 10_1101-2021_01_07_425794 12 13 , , , 10_1101-2021_01_07_425794 12 14 2021 2021 CD 10_1101-2021_01_07_425794 12 15 . . . 10_1101-2021_01_07_425794 12 16 ; ; : 10_1101-2021_01_07_425794 12 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 12 18 : : : 10_1101-2021_01_07_425794 12 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 12 20 preprint preprint JJ 10_1101-2021_01_07_425794 12 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 12 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 12 23 1 1 CD 10_1101-2021_01_07_425794 12 24 INTRODUCTION introduction NN 10_1101-2021_01_07_425794 12 25 Gene gene NN 10_1101-2021_01_07_425794 12 26 expression expression NN 10_1101-2021_01_07_425794 12 27 profiling profiling NN 10_1101-2021_01_07_425794 12 28 using use VBG 10_1101-2021_01_07_425794 12 29 RNA RNA NNP 10_1101-2021_01_07_425794 12 30 sequencing sequencing NN 10_1101-2021_01_07_425794 12 31 ( ( -LRB- 10_1101-2021_01_07_425794 12 32 RNA RNA NNP 10_1101-2021_01_07_425794 12 33 - - HYPH 10_1101-2021_01_07_425794 12 34 seq seq NNP 10_1101-2021_01_07_425794 12 35 ) ) -RRB- 10_1101-2021_01_07_425794 12 36 is be VBZ 10_1101-2021_01_07_425794 12 37 a a DT 10_1101-2021_01_07_425794 12 38 core core NN 10_1101-2021_01_07_425794 12 39 activity activity NN 10_1101-2021_01_07_425794 12 40 in in IN 10_1101-2021_01_07_425794 12 41 molec- molec- NNP 10_1101-2021_01_07_425794 12 42 ular ular NNP 10_1101-2021_01_07_425794 12 43 biology biology NN 10_1101-2021_01_07_425794 12 44 . . . 10_1101-2021_01_07_425794 13 1 Comprehensive comprehensive JJ 10_1101-2021_01_07_425794 13 2 gene gene NN 10_1101-2021_01_07_425794 13 3 expression expression NN 10_1101-2021_01_07_425794 13 4 analysis analysis NN 10_1101-2021_01_07_425794 13 5 in in IN 10_1101-2021_01_07_425794 13 6 various various JJ 10_1101-2021_01_07_425794 13 7 settings setting NNS 10_1101-2021_01_07_425794 13 8 is be VBZ 10_1101-2021_01_07_425794 13 9 important important JJ 10_1101-2021_01_07_425794 13 10 for for IN 10_1101-2021_01_07_425794 13 11 generating generate VBG 10_1101-2021_01_07_425794 13 12 hypotheses hypothesis NNS 10_1101-2021_01_07_425794 13 13 for for IN 10_1101-2021_01_07_425794 13 14 ongoing ongoing JJ 10_1101-2021_01_07_425794 13 15 research research NN 10_1101-2021_01_07_425794 13 16 , , , 10_1101-2021_01_07_425794 13 17 investigating investigate VBG 10_1101-2021_01_07_425794 13 18 drug drug NN 10_1101-2021_01_07_425794 13 19 - - HYPH 10_1101-2021_01_07_425794 13 20 effects effect NNS 10_1101-2021_01_07_425794 13 21 in in IN 10_1101-2021_01_07_425794 13 22 biological biological JJ 10_1101-2021_01_07_425794 13 23 or or CC 10_1101-2021_01_07_425794 13 24 clinical clinical JJ 10_1101-2021_01_07_425794 13 25 settings setting NNS 10_1101-2021_01_07_425794 13 26 and and CC 10_1101-2021_01_07_425794 13 27 as as IN 10_1101-2021_01_07_425794 13 28 a a DT 10_1101-2021_01_07_425794 13 29 diagnostic diagnostic JJ 10_1101-2021_01_07_425794 13 30 tool tool NN 10_1101-2021_01_07_425794 13 31 . . . 10_1101-2021_01_07_425794 14 1 In in IN 10_1101-2021_01_07_425794 14 2 this this DT 10_1101-2021_01_07_425794 14 3 paper paper NN 10_1101-2021_01_07_425794 14 4 , , , 10_1101-2021_01_07_425794 14 5 we -PRON- PRP 10_1101-2021_01_07_425794 14 6 explore explore VBP 10_1101-2021_01_07_425794 14 7 the the DT 10_1101-2021_01_07_425794 14 8 fact fact NN 10_1101-2021_01_07_425794 14 9 that that IN 10_1101-2021_01_07_425794 14 10 a a DT 10_1101-2021_01_07_425794 14 11 popular popular JJ 10_1101-2021_01_07_425794 14 12 approach approach NN 10_1101-2021_01_07_425794 14 13 in in IN 10_1101-2021_01_07_425794 14 14 gene gene NN 10_1101-2021_01_07_425794 14 15 - - HYPH 10_1101-2021_01_07_425794 14 16 level level NN 10_1101-2021_01_07_425794 14 17 quantification quantification NN 10_1101-2021_01_07_425794 14 18 from from IN 10_1101-2021_01_07_425794 14 19 RNA RNA NNP 10_1101-2021_01_07_425794 14 20 - - HYPH 10_1101-2021_01_07_425794 14 21 seq seq NN 10_1101-2021_01_07_425794 14 22 data datum NNS 10_1101-2021_01_07_425794 14 23 involves involve VBZ 10_1101-2021_01_07_425794 14 24 mapping mapping NN 10_1101-2021_01_07_425794 14 25 reads read VBZ 10_1101-2021_01_07_425794 14 26 to to IN 10_1101-2021_01_07_425794 14 27 a a DT 10_1101-2021_01_07_425794 14 28 ref- ref- JJ 10_1101-2021_01_07_425794 14 29 erence erence NN 10_1101-2021_01_07_425794 14 30 genome genome JJ 10_1101-2021_01_07_425794 14 31 and and CC 10_1101-2021_01_07_425794 14 32 then then RB 10_1101-2021_01_07_425794 14 33 counting count VBG 10_1101-2021_01_07_425794 14 34 mapped map VBN 10_1101-2021_01_07_425794 14 35 reads read NNS 10_1101-2021_01_07_425794 14 36 associated associate VBN 10_1101-2021_01_07_425794 14 37 with with IN 10_1101-2021_01_07_425794 14 38 each each DT 10_1101-2021_01_07_425794 14 39 gene gene NN 10_1101-2021_01_07_425794 14 40 [ [ -LRB- 10_1101-2021_01_07_425794 14 41 1 1 CD 10_1101-2021_01_07_425794 14 42 , , , 10_1101-2021_01_07_425794 14 43 2 2 CD 10_1101-2021_01_07_425794 14 44 , , , 10_1101-2021_01_07_425794 14 45 3 3 CD 10_1101-2021_01_07_425794 14 46 , , , 10_1101-2021_01_07_425794 14 47 4 4 CD 10_1101-2021_01_07_425794 14 48 , , , 10_1101-2021_01_07_425794 14 49 5 5 CD 10_1101-2021_01_07_425794 14 50 ] ] -RRB- 10_1101-2021_01_07_425794 14 51 . . . 10_1101-2021_01_07_425794 15 1 The the DT 10_1101-2021_01_07_425794 15 2 process process NN 10_1101-2021_01_07_425794 15 3 of of IN 10_1101-2021_01_07_425794 15 4 counting count VBG 10_1101-2021_01_07_425794 15 5 mapped map VBN 10_1101-2021_01_07_425794 15 6 reads read NNS 10_1101-2021_01_07_425794 15 7 to to IN 10_1101-2021_01_07_425794 15 8 genes gene NNS 10_1101-2021_01_07_425794 15 9 requires require VBZ 10_1101-2021_01_07_425794 15 10 a a DT 10_1101-2021_01_07_425794 15 11 database database NN 10_1101-2021_01_07_425794 15 12 of of IN 10_1101-2021_01_07_425794 15 13 known know VBN 10_1101-2021_01_07_425794 15 14 genes gene NNS 10_1101-2021_01_07_425794 15 15 . . . 10_1101-2021_01_07_425794 16 1 A a DT 10_1101-2021_01_07_425794 16 2 gene gene NN 10_1101-2021_01_07_425794 16 3 is be VBZ 10_1101-2021_01_07_425794 16 4 only only RB 10_1101-2021_01_07_425794 16 5 quantified quantify VBN 10_1101-2021_01_07_425794 16 6 if if IN 10_1101-2021_01_07_425794 16 7 it -PRON- PRP 10_1101-2021_01_07_425794 16 8 or or CC 10_1101-2021_01_07_425794 16 9 its -PRON- PRP$ 10_1101-2021_01_07_425794 16 10 components component NNS 10_1101-2021_01_07_425794 16 11 have have VBP 10_1101-2021_01_07_425794 16 12 genomic genomic JJ 10_1101-2021_01_07_425794 16 13 coordinates coordinate NNS 10_1101-2021_01_07_425794 16 14 already already RB 10_1101-2021_01_07_425794 16 15 defined define VBN 10_1101-2021_01_07_425794 16 16 with with IN 10_1101-2021_01_07_425794 16 17 respect respect NN 10_1101-2021_01_07_425794 16 18 to to IN 10_1101-2021_01_07_425794 16 19 the the DT 10_1101-2021_01_07_425794 16 20 genome genome JJ 10_1101-2021_01_07_425794 16 21 sequence sequence NN 10_1101-2021_01_07_425794 16 22 in in IN 10_1101-2021_01_07_425794 16 23 a a DT 10_1101-2021_01_07_425794 16 24 process process NN 10_1101-2021_01_07_425794 16 25 called call VBN 10_1101-2021_01_07_425794 16 26 annotation annotation NN 10_1101-2021_01_07_425794 16 27 . . . 10_1101-2021_01_07_425794 17 1 For for IN 10_1101-2021_01_07_425794 17 2 each each DT 10_1101-2021_01_07_425794 17 3 genome genome JJ 10_1101-2021_01_07_425794 17 4 annotation annotation NN 10_1101-2021_01_07_425794 17 5 model model NN 10_1101-2021_01_07_425794 17 6 , , , 10_1101-2021_01_07_425794 17 7 a a DT 10_1101-2021_01_07_425794 17 8 different different JJ 10_1101-2021_01_07_425794 17 9 set set NN 10_1101-2021_01_07_425794 17 10 of of IN 10_1101-2021_01_07_425794 17 11 annotation annotation NN 10_1101-2021_01_07_425794 17 12 techniques technique NNS 10_1101-2021_01_07_425794 17 13 and and CC 10_1101-2021_01_07_425794 17 14 information information NN 10_1101-2021_01_07_425794 17 15 sources source NNS 10_1101-2021_01_07_425794 17 16 are be VBP 10_1101-2021_01_07_425794 17 17 used use VBN 10_1101-2021_01_07_425794 17 18 and and CC 10_1101-2021_01_07_425794 17 19 as as IN 10_1101-2021_01_07_425794 17 20 such such JJ 10_1101-2021_01_07_425794 17 21 , , , 10_1101-2021_01_07_425794 17 22 these these DT 10_1101-2021_01_07_425794 17 23 annotations annotation NNS 10_1101-2021_01_07_425794 17 24 vary vary VBP 10_1101-2021_01_07_425794 17 25 in in IN 10_1101-2021_01_07_425794 17 26 terms term NNS 10_1101-2021_01_07_425794 17 27 of of IN 10_1101-2021_01_07_425794 17 28 comprehensiveness comprehensiveness NN 10_1101-2021_01_07_425794 17 29 and and CC 10_1101-2021_01_07_425794 17 30 accuracy accuracy NN 10_1101-2021_01_07_425794 17 31 of of IN 10_1101-2021_01_07_425794 17 32 annotated annotate VBN 10_1101-2021_01_07_425794 17 33 genomic genomic JJ 10_1101-2021_01_07_425794 17 34 features feature NNS 10_1101-2021_01_07_425794 17 35 . . . 10_1101-2021_01_07_425794 18 1 Annotation annotation NN 10_1101-2021_01_07_425794 18 2 techniques technique NNS 10_1101-2021_01_07_425794 18 3 often often RB 10_1101-2021_01_07_425794 18 4 include include VBP 10_1101-2021_01_07_425794 18 5 computer computer NN 10_1101-2021_01_07_425794 18 6 - - HYPH 10_1101-2021_01_07_425794 18 7 based base VBN 10_1101-2021_01_07_425794 18 8 predic- predic- NN 10_1101-2021_01_07_425794 18 9 tions tion NNS 10_1101-2021_01_07_425794 18 10 and/or and/or CC 10_1101-2021_01_07_425794 18 11 evidence evidence NN 10_1101-2021_01_07_425794 18 12 - - HYPH 10_1101-2021_01_07_425794 18 13 based base VBN 10_1101-2021_01_07_425794 18 14 techniques technique NNS 10_1101-2021_01_07_425794 18 15 such such JJ 10_1101-2021_01_07_425794 18 16 as as IN 10_1101-2021_01_07_425794 18 17 manual manual JJ 10_1101-2021_01_07_425794 18 18 curation curation NN 10_1101-2021_01_07_425794 18 19 [ [ -LRB- 10_1101-2021_01_07_425794 18 20 6 6 CD 10_1101-2021_01_07_425794 18 21 , , , 10_1101-2021_01_07_425794 18 22 7 7 CD 10_1101-2021_01_07_425794 18 23 ] ] -RRB- 10_1101-2021_01_07_425794 18 24 . . . 10_1101-2021_01_07_425794 19 1 Computer computer NN 10_1101-2021_01_07_425794 19 2 - - HYPH 10_1101-2021_01_07_425794 19 3 based base VBN 10_1101-2021_01_07_425794 19 4 predictions prediction NNS 10_1101-2021_01_07_425794 19 5 result result VBP 10_1101-2021_01_07_425794 19 6 in in IN 10_1101-2021_01_07_425794 19 7 more more JJR 10_1101-2021_01_07_425794 19 8 complex complex JJ 10_1101-2021_01_07_425794 19 9 gene gene NN 10_1101-2021_01_07_425794 19 10 models model NNS 10_1101-2021_01_07_425794 19 11 that that WDT 10_1101-2021_01_07_425794 19 12 have have VBP 10_1101-2021_01_07_425794 19 13 a a DT 10_1101-2021_01_07_425794 19 14 higher high JJR 10_1101-2021_01_07_425794 19 15 proportion proportion NN 10_1101-2021_01_07_425794 19 16 of of IN 10_1101-2021_01_07_425794 19 17 predic- predic- JJ 10_1101-2021_01_07_425794 19 18 tive tive JJ 10_1101-2021_01_07_425794 19 19 genomic genomic JJ 10_1101-2021_01_07_425794 19 20 features feature NNS 10_1101-2021_01_07_425794 19 21 while while IN 10_1101-2021_01_07_425794 19 22 evidence evidence NN 10_1101-2021_01_07_425794 19 23 - - HYPH 10_1101-2021_01_07_425794 19 24 based base VBN 10_1101-2021_01_07_425794 19 25 generated generate VBN 10_1101-2021_01_07_425794 19 26 gene gene NN 10_1101-2021_01_07_425794 19 27 models model NNS 10_1101-2021_01_07_425794 19 28 are be VBP 10_1101-2021_01_07_425794 19 29 simpler simple JJR 10_1101-2021_01_07_425794 19 30 with with IN 10_1101-2021_01_07_425794 19 31 fewer few JJR 10_1101-2021_01_07_425794 19 32 genes gene NNS 10_1101-2021_01_07_425794 19 33 and and CC 10_1101-2021_01_07_425794 19 34 isoforms isoform NNS 10_1101-2021_01_07_425794 19 35 . . . 10_1101-2021_01_07_425794 20 1 Common common JJ 10_1101-2021_01_07_425794 20 2 annotation annotation NN 10_1101-2021_01_07_425794 20 3 models model NNS 10_1101-2021_01_07_425794 20 4 for for IN 10_1101-2021_01_07_425794 20 5 human human NN 10_1101-2021_01_07_425794 20 6 and and CC 10_1101-2021_01_07_425794 20 7 mouse mouse NN 10_1101-2021_01_07_425794 20 8 genomes genome NNS 10_1101-2021_01_07_425794 20 9 include include VBP 10_1101-2021_01_07_425794 20 10 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 20 11 [ [ -LRB- 10_1101-2021_01_07_425794 20 12 8 8 CD 10_1101-2021_01_07_425794 20 13 ] ] -RRB- 10_1101-2021_01_07_425794 20 14 , , , 10_1101-2021_01_07_425794 20 15 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 20 16 [ [ -LRB- 10_1101-2021_01_07_425794 20 17 9 9 CD 10_1101-2021_01_07_425794 20 18 ] ] -RRB- 10_1101-2021_01_07_425794 20 19 , , , 10_1101-2021_01_07_425794 20 20 GENCODE GENCODE NNP 10_1101-2021_01_07_425794 20 21 [ [ -LRB- 10_1101-2021_01_07_425794 20 22 10 10 CD 10_1101-2021_01_07_425794 20 23 ] ] -RRB- 10_1101-2021_01_07_425794 20 24 and and CC 10_1101-2021_01_07_425794 20 25 UCSC UCSC NNP 10_1101-2021_01_07_425794 20 26 [ [ -LRB- 10_1101-2021_01_07_425794 20 27 11 11 CD 10_1101-2021_01_07_425794 20 28 ] ] -RRB- 10_1101-2021_01_07_425794 20 29 annotations annotation NNS 10_1101-2021_01_07_425794 20 30 . . . 10_1101-2021_01_07_425794 21 1 Annotations annotation NNS 10_1101-2021_01_07_425794 21 2 are be VBP 10_1101-2021_01_07_425794 21 3 , , , 10_1101-2021_01_07_425794 21 4 therefore therefore RB 10_1101-2021_01_07_425794 21 5 , , , 10_1101-2021_01_07_425794 21 6 an an DT 10_1101-2021_01_07_425794 21 7 important important JJ 10_1101-2021_01_07_425794 21 8 component component NN 10_1101-2021_01_07_425794 21 9 in in IN 10_1101-2021_01_07_425794 21 10 an an DT 10_1101-2021_01_07_425794 21 11 RNA RNA NNP 10_1101-2021_01_07_425794 21 12 - - HYPH 10_1101-2021_01_07_425794 21 13 seq seq NN 10_1101-2021_01_07_425794 21 14 analysis analysis NN 10_1101-2021_01_07_425794 21 15 as as IN 10_1101-2021_01_07_425794 21 16 the the DT 10_1101-2021_01_07_425794 21 17 results result NNS 10_1101-2021_01_07_425794 21 18 are be VBP 10_1101-2021_01_07_425794 21 19 dependent dependent JJ 10_1101-2021_01_07_425794 21 20 on on IN 10_1101-2021_01_07_425794 21 21 what what WP 10_1101-2021_01_07_425794 21 22 is be VBZ 10_1101-2021_01_07_425794 21 23 known know VBN 10_1101-2021_01_07_425794 21 24 in in IN 10_1101-2021_01_07_425794 21 25 the the DT 10_1101-2021_01_07_425794 21 26 annotation annotation NN 10_1101-2021_01_07_425794 21 27 database database NN 10_1101-2021_01_07_425794 21 28 . . . 10_1101-2021_01_07_425794 22 1 Despite despite IN 10_1101-2021_01_07_425794 22 2 the the DT 10_1101-2021_01_07_425794 22 3 importance importance NN 10_1101-2021_01_07_425794 22 4 of of IN 10_1101-2021_01_07_425794 22 5 gene gene NN 10_1101-2021_01_07_425794 22 6 annotations annotation NNS 10_1101-2021_01_07_425794 22 7 in in IN 10_1101-2021_01_07_425794 22 8 RNA RNA NNP 10_1101-2021_01_07_425794 22 9 - - HYPH 10_1101-2021_01_07_425794 22 10 seq seq NN 10_1101-2021_01_07_425794 22 11 data datum NNS 10_1101-2021_01_07_425794 22 12 analysis analysis NN 10_1101-2021_01_07_425794 22 13 , , , 10_1101-2021_01_07_425794 22 14 very very RB 10_1101-2021_01_07_425794 22 15 little little JJ 10_1101-2021_01_07_425794 22 16 re- re- JJ 10_1101-2021_01_07_425794 22 17 search search NN 10_1101-2021_01_07_425794 22 18 has have VBZ 10_1101-2021_01_07_425794 22 19 been be VBN 10_1101-2021_01_07_425794 22 20 conducted conduct VBN 10_1101-2021_01_07_425794 22 21 to to TO 10_1101-2021_01_07_425794 22 22 examine examine VB 10_1101-2021_01_07_425794 22 23 how how WRB 10_1101-2021_01_07_425794 22 24 differences difference NNS 10_1101-2021_01_07_425794 22 25 in in IN 10_1101-2021_01_07_425794 22 26 annotations annotation NNS 10_1101-2021_01_07_425794 22 27 impact impact NN 10_1101-2021_01_07_425794 22 28 on on IN 10_1101-2021_01_07_425794 22 29 gene gene NN 10_1101-2021_01_07_425794 22 30 expression expression NN 10_1101-2021_01_07_425794 22 31 quantification quantification NN 10_1101-2021_01_07_425794 22 32 , , , 10_1101-2021_01_07_425794 22 33 which which WDT 10_1101-2021_01_07_425794 22 34 is be VBZ 10_1101-2021_01_07_425794 22 35 crucial crucial JJ 10_1101-2021_01_07_425794 22 36 for for IN 10_1101-2021_01_07_425794 22 37 downstream downstream JJ 10_1101-2021_01_07_425794 22 38 analyses analysis NNS 10_1101-2021_01_07_425794 22 39 such such JJ 10_1101-2021_01_07_425794 22 40 as as IN 10_1101-2021_01_07_425794 22 41 discovery discovery NN 10_1101-2021_01_07_425794 22 42 of of IN 10_1101-2021_01_07_425794 22 43 differentially differentially RB 10_1101-2021_01_07_425794 22 44 expressed express VBN 10_1101-2021_01_07_425794 22 45 genes gene NNS 10_1101-2021_01_07_425794 22 46 and and CC 10_1101-2021_01_07_425794 22 47 identification identification NN 10_1101-2021_01_07_425794 22 48 of of IN 10_1101-2021_01_07_425794 22 49 perturbed perturb VBN 10_1101-2021_01_07_425794 22 50 pathways pathway NNS 10_1101-2021_01_07_425794 22 51 . . . 10_1101-2021_01_07_425794 23 1 Previous previous JJ 10_1101-2021_01_07_425794 23 2 studies study NNS 10_1101-2021_01_07_425794 23 3 compared compare VBD 10_1101-2021_01_07_425794 23 4 the the DT 10_1101-2021_01_07_425794 23 5 effect effect NN 10_1101-2021_01_07_425794 23 6 of of IN 10_1101-2021_01_07_425794 23 7 human human JJ 10_1101-2021_01_07_425794 23 8 genome genome JJ 10_1101-2021_01_07_425794 23 9 annotations annotation NNS 10_1101-2021_01_07_425794 23 10 from from IN 10_1101-2021_01_07_425794 23 11 popular popular JJ 10_1101-2021_01_07_425794 23 12 databases database NNS 10_1101-2021_01_07_425794 23 13 including include VBG 10_1101-2021_01_07_425794 23 14 En- en- XX 10_1101-2021_01_07_425794 23 15 sembl sembl NN 10_1101-2021_01_07_425794 23 16 , , , 10_1101-2021_01_07_425794 23 17 GENCODE GENCODE NNP 10_1101-2021_01_07_425794 23 18 and and CC 10_1101-2021_01_07_425794 23 19 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 23 20 on on IN 10_1101-2021_01_07_425794 23 21 various various JJ 10_1101-2021_01_07_425794 23 22 aspects aspect NNS 10_1101-2021_01_07_425794 23 23 of of IN 10_1101-2021_01_07_425794 23 24 RNA RNA NNP 10_1101-2021_01_07_425794 23 25 - - HYPH 10_1101-2021_01_07_425794 23 26 seq seq NN 10_1101-2021_01_07_425794 23 27 analysis analysis NN 10_1101-2021_01_07_425794 23 28 and and CC 10_1101-2021_01_07_425794 23 29 they -PRON- PRP 10_1101-2021_01_07_425794 23 30 showed show VBD 10_1101-2021_01_07_425794 23 31 that that IN 10_1101-2021_01_07_425794 23 32 the the DT 10_1101-2021_01_07_425794 23 33 choice choice NN 10_1101-2021_01_07_425794 23 34 of of IN 10_1101-2021_01_07_425794 23 35 annotations annotation NNS 10_1101-2021_01_07_425794 23 36 had have VBD 10_1101-2021_01_07_425794 23 37 an an DT 10_1101-2021_01_07_425794 23 38 impact impact NN 10_1101-2021_01_07_425794 23 39 on on IN 10_1101-2021_01_07_425794 23 40 gene gene NN 10_1101-2021_01_07_425794 23 41 - - HYPH 10_1101-2021_01_07_425794 23 42 level level NN 10_1101-2021_01_07_425794 23 43 quantification quantification NN 10_1101-2021_01_07_425794 23 44 in in IN 10_1101-2021_01_07_425794 23 45 the the DT 10_1101-2021_01_07_425794 23 46 RNA- RNA- NNP 10_1101-2021_01_07_425794 23 47 seq seq NN 10_1101-2021_01_07_425794 23 48 analysis analysis NN 10_1101-2021_01_07_425794 23 49 [ [ -LRB- 10_1101-2021_01_07_425794 23 50 12 12 CD 10_1101-2021_01_07_425794 23 51 , , , 10_1101-2021_01_07_425794 23 52 13 13 CD 10_1101-2021_01_07_425794 23 53 ] ] -RRB- 10_1101-2021_01_07_425794 23 54 . . . 10_1101-2021_01_07_425794 24 1 However however RB 10_1101-2021_01_07_425794 24 2 , , , 10_1101-2021_01_07_425794 24 3 these these DT 10_1101-2021_01_07_425794 24 4 studies study NNS 10_1101-2021_01_07_425794 24 5 are be VBP 10_1101-2021_01_07_425794 24 6 out out IN 10_1101-2021_01_07_425794 24 7 of of IN 10_1101-2021_01_07_425794 24 8 date date NN 10_1101-2021_01_07_425794 24 9 as as IN 10_1101-2021_01_07_425794 24 10 they -PRON- PRP 10_1101-2021_01_07_425794 24 11 were be VBD 10_1101-2021_01_07_425794 24 12 based base VBN 10_1101-2021_01_07_425794 24 13 on on IN 10_1101-2021_01_07_425794 24 14 old old JJ 10_1101-2021_01_07_425794 24 15 annotations annotation NNS 10_1101-2021_01_07_425794 24 16 and and CC 10_1101-2021_01_07_425794 24 17 they -PRON- PRP 10_1101-2021_01_07_425794 24 18 also also RB 10_1101-2021_01_07_425794 24 19 lacked lack VBD 10_1101-2021_01_07_425794 24 20 a a DT 10_1101-2021_01_07_425794 24 21 reliable reliable JJ 10_1101-2021_01_07_425794 24 22 ground ground NN 10_1101-2021_01_07_425794 24 23 truth truth NN 10_1101-2021_01_07_425794 24 24 for for IN 10_1101-2021_01_07_425794 24 25 assessing assess VBG 10_1101-2021_01_07_425794 24 26 the the DT 10_1101-2021_01_07_425794 24 27 impact impact NN 10_1101-2021_01_07_425794 24 28 of of IN 10_1101-2021_01_07_425794 24 29 annotation annotation NN 10_1101-2021_01_07_425794 24 30 . . . 10_1101-2021_01_07_425794 25 1 Major major JJ 10_1101-2021_01_07_425794 25 2 annotation annotation NN 10_1101-2021_01_07_425794 25 3 databases database NNS 10_1101-2021_01_07_425794 25 4 have have VBP 10_1101-2021_01_07_425794 25 5 undergone undergo VBN 10_1101-2021_01_07_425794 25 6 significant significant JJ 10_1101-2021_01_07_425794 25 7 expansions expansion NNS 10_1101-2021_01_07_425794 25 8 over over IN 10_1101-2021_01_07_425794 25 9 the the DT 10_1101-2021_01_07_425794 25 10 years year NNS 10_1101-2021_01_07_425794 25 11 , , , 10_1101-2021_01_07_425794 25 12 thanks thank NNS 10_1101-2021_01_07_425794 25 13 to to IN 10_1101-2021_01_07_425794 25 14 the the DT 10_1101-2021_01_07_425794 25 15 wide wide JJ 10_1101-2021_01_07_425794 25 16 application application NN 10_1101-2021_01_07_425794 25 17 of of IN 10_1101-2021_01_07_425794 25 18 sequencing sequence VBG 10_1101-2021_01_07_425794 25 19 technologies technology NNS 10_1101-2021_01_07_425794 25 20 and and CC 10_1101-2021_01_07_425794 25 21 the the DT 10_1101-2021_01_07_425794 25 22 massive massive JJ 10_1101-2021_01_07_425794 25 23 amount amount NN 10_1101-2021_01_07_425794 25 24 of of IN 10_1101-2021_01_07_425794 25 25 se- se- NN 10_1101-2021_01_07_425794 25 26 quencing quencing JJ 10_1101-2021_01_07_425794 25 27 data datum NNS 10_1101-2021_01_07_425794 25 28 that that WDT 10_1101-2021_01_07_425794 25 29 have have VBP 10_1101-2021_01_07_425794 25 30 been be VBN 10_1101-2021_01_07_425794 25 31 generated generate VBN 10_1101-2021_01_07_425794 25 32 across across IN 10_1101-2021_01_07_425794 25 33 the the DT 10_1101-2021_01_07_425794 25 34 world world NN 10_1101-2021_01_07_425794 25 35 . . . 10_1101-2021_01_07_425794 26 1 However however RB 10_1101-2021_01_07_425794 26 2 , , , 10_1101-2021_01_07_425794 26 3 it -PRON- PRP 10_1101-2021_01_07_425794 26 4 is be VBZ 10_1101-2021_01_07_425794 26 5 unclear unclear JJ 10_1101-2021_01_07_425794 26 6 whether whether IN 10_1101-2021_01_07_425794 26 7 the the DT 10_1101-2021_01_07_425794 26 8 quality quality NN 10_1101-2021_01_07_425794 26 9 of of IN 10_1101-2021_01_07_425794 26 10 gene gene NN 10_1101-2021_01_07_425794 26 11 annotations annotation NNS 10_1101-2021_01_07_425794 26 12 have have VBP 10_1101-2021_01_07_425794 26 13 been be VBN 10_1101-2021_01_07_425794 26 14 successfully successfully RB 10_1101-2021_01_07_425794 26 15 maintained maintain VBN 10_1101-2021_01_07_425794 26 16 . . . 10_1101-2021_01_07_425794 27 1 A a DT 10_1101-2021_01_07_425794 27 2 recent recent JJ 10_1101-2021_01_07_425794 27 3 study study NN 10_1101-2021_01_07_425794 27 4 sug- sug- NN 10_1101-2021_01_07_425794 27 5 gested geste VBD 10_1101-2021_01_07_425794 27 6 that that IN 10_1101-2021_01_07_425794 27 7 gene gene NN 10_1101-2021_01_07_425794 27 8 annotations annotation NNS 10_1101-2021_01_07_425794 27 9 have have VBP 10_1101-2021_01_07_425794 27 10 become become VBN 10_1101-2021_01_07_425794 27 11 less less RBR 10_1101-2021_01_07_425794 27 12 accurate accurate JJ 10_1101-2021_01_07_425794 27 13 and and CC 10_1101-2021_01_07_425794 27 14 lagging lagging JJ 10_1101-2021_01_07_425794 27 15 during during IN 10_1101-2021_01_07_425794 27 16 this this DT 10_1101-2021_01_07_425794 27 17 expansion expansion NN 10_1101-2021_01_07_425794 27 18 [ [ -LRB- 10_1101-2021_01_07_425794 27 19 6 6 CD 10_1101-2021_01_07_425794 27 20 ] ] -RRB- 10_1101-2021_01_07_425794 27 21 . . . 10_1101-2021_01_07_425794 28 1 This this DT 10_1101-2021_01_07_425794 28 2 can can MD 10_1101-2021_01_07_425794 28 3 be be VB 10_1101-2021_01_07_425794 28 4 attributed attribute VBN 10_1101-2021_01_07_425794 28 5 to to IN 10_1101-2021_01_07_425794 28 6 the the DT 10_1101-2021_01_07_425794 28 7 errors error NNS 10_1101-2021_01_07_425794 28 8 from from IN 10_1101-2021_01_07_425794 28 9 sequencing sequence VBG 10_1101-2021_01_07_425794 28 10 experiments experiment NNS 10_1101-2021_01_07_425794 28 11 , , , 10_1101-2021_01_07_425794 28 12 sequence sequence NN 10_1101-2021_01_07_425794 28 13 analysis analysis NN 10_1101-2021_01_07_425794 28 14 2 2 CD 10_1101-2021_01_07_425794 28 15 .CC .CC , 10_1101-2021_01_07_425794 28 16 - - HYPH 10_1101-2021_01_07_425794 28 17 BY by IN 10_1101-2021_01_07_425794 28 18 - - HYPH 10_1101-2021_01_07_425794 28 19 NC NC NNP 10_1101-2021_01_07_425794 28 20 - - HYPH 10_1101-2021_01_07_425794 28 21 ND ND NNP 10_1101-2021_01_07_425794 28 22 4.0 4.0 CD 10_1101-2021_01_07_425794 28 23 International international JJ 10_1101-2021_01_07_425794 28 24 licensemade licensemade NN 10_1101-2021_01_07_425794 28 25 available available JJ 10_1101-2021_01_07_425794 28 26 under under IN 10_1101-2021_01_07_425794 28 27 a a DT 10_1101-2021_01_07_425794 28 28 ( ( -LRB- 10_1101-2021_01_07_425794 28 29 which which WDT 10_1101-2021_01_07_425794 28 30 was be VBD 10_1101-2021_01_07_425794 28 31 not not RB 10_1101-2021_01_07_425794 28 32 certified certify VBN 10_1101-2021_01_07_425794 28 33 by by IN 10_1101-2021_01_07_425794 28 34 peer peer NN 10_1101-2021_01_07_425794 28 35 review review NN 10_1101-2021_01_07_425794 28 36 ) ) -RRB- 10_1101-2021_01_07_425794 28 37 is be VBZ 10_1101-2021_01_07_425794 28 38 the the DT 10_1101-2021_01_07_425794 28 39 author author NN 10_1101-2021_01_07_425794 28 40 / / SYM 10_1101-2021_01_07_425794 28 41 funder funder NN 10_1101-2021_01_07_425794 28 42 , , , 10_1101-2021_01_07_425794 28 43 who who WP 10_1101-2021_01_07_425794 28 44 has have VBZ 10_1101-2021_01_07_425794 28 45 granted grant VBN 10_1101-2021_01_07_425794 28 46 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 28 47 a a DT 10_1101-2021_01_07_425794 28 48 license license NN 10_1101-2021_01_07_425794 28 49 to to TO 10_1101-2021_01_07_425794 28 50 display display VB 10_1101-2021_01_07_425794 28 51 the the DT 10_1101-2021_01_07_425794 28 52 preprint preprint NN 10_1101-2021_01_07_425794 28 53 in in IN 10_1101-2021_01_07_425794 28 54 perpetuity perpetuity NN 10_1101-2021_01_07_425794 28 55 . . . 10_1101-2021_01_07_425794 29 1 It -PRON- PRP 10_1101-2021_01_07_425794 29 2 is be VBZ 10_1101-2021_01_07_425794 29 3 The the DT 10_1101-2021_01_07_425794 29 4 copyright copyright NN 10_1101-2021_01_07_425794 29 5 holder holder NN 10_1101-2021_01_07_425794 29 6 for for IN 10_1101-2021_01_07_425794 29 7 this this DT 10_1101-2021_01_07_425794 29 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 29 9 version version NN 10_1101-2021_01_07_425794 29 10 posted post VBD 10_1101-2021_01_07_425794 29 11 January January NNP 10_1101-2021_01_07_425794 29 12 8 8 CD 10_1101-2021_01_07_425794 29 13 , , , 10_1101-2021_01_07_425794 29 14 2021 2021 CD 10_1101-2021_01_07_425794 29 15 . . . 10_1101-2021_01_07_425794 29 16 ; ; : 10_1101-2021_01_07_425794 29 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 29 18 : : : 10_1101-2021_01_07_425794 29 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 29 20 preprint preprint JJ 10_1101-2021_01_07_425794 29 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 29 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 29 23 or or CC 10_1101-2021_01_07_425794 29 24 automation automation NN 10_1101-2021_01_07_425794 29 25 in in IN 10_1101-2021_01_07_425794 29 26 the the DT 10_1101-2021_01_07_425794 29 27 annotation annotation NN 10_1101-2021_01_07_425794 29 28 process process NN 10_1101-2021_01_07_425794 29 29 . . . 10_1101-2021_01_07_425794 30 1 It -PRON- PRP 10_1101-2021_01_07_425794 30 2 is be VBZ 10_1101-2021_01_07_425794 30 3 important important JJ 10_1101-2021_01_07_425794 30 4 to to TO 10_1101-2021_01_07_425794 30 5 systematically systematically RB 10_1101-2021_01_07_425794 30 6 assess assess VB 10_1101-2021_01_07_425794 30 7 the the DT 10_1101-2021_01_07_425794 30 8 accuracy accuracy NN 10_1101-2021_01_07_425794 30 9 of of IN 10_1101-2021_01_07_425794 30 10 the the DT 10_1101-2021_01_07_425794 30 11 new new JJ 10_1101-2021_01_07_425794 30 12 gene gene NN 10_1101-2021_01_07_425794 30 13 annotations annotation NNS 10_1101-2021_01_07_425794 30 14 generated generate VBN 10_1101-2021_01_07_425794 30 15 in in IN 10_1101-2021_01_07_425794 30 16 recent recent JJ 10_1101-2021_01_07_425794 30 17 years year NNS 10_1101-2021_01_07_425794 30 18 to to TO 10_1101-2021_01_07_425794 30 19 ensure ensure VB 10_1101-2021_01_07_425794 30 20 the the DT 10_1101-2021_01_07_425794 30 21 popular popular JJ 10_1101-2021_01_07_425794 30 22 annotation annotation NN 10_1101-2021_01_07_425794 30 23 databases database NNS 10_1101-2021_01_07_425794 30 24 can can MD 10_1101-2021_01_07_425794 30 25 continue continue VB 10_1101-2021_01_07_425794 30 26 to to TO 10_1101-2021_01_07_425794 30 27 be be VB 10_1101-2021_01_07_425794 30 28 utilized utilize VBN 10_1101-2021_01_07_425794 30 29 by by IN 10_1101-2021_01_07_425794 30 30 the the DT 10_1101-2021_01_07_425794 30 31 community community NN 10_1101-2021_01_07_425794 30 32 for for IN 10_1101-2021_01_07_425794 30 33 RNA RNA NNP 10_1101-2021_01_07_425794 30 34 - - HYPH 10_1101-2021_01_07_425794 30 35 seq seq NN 10_1101-2021_01_07_425794 30 36 analysis analysis NN 10_1101-2021_01_07_425794 30 37 . . . 10_1101-2021_01_07_425794 31 1 Furthermore furthermore RB 10_1101-2021_01_07_425794 31 2 , , , 10_1101-2021_01_07_425794 31 3 the the DT 10_1101-2021_01_07_425794 31 4 use use NN 10_1101-2021_01_07_425794 31 5 of of IN 10_1101-2021_01_07_425794 31 6 different different JJ 10_1101-2021_01_07_425794 31 7 annotations annotation NNS 10_1101-2021_01_07_425794 31 8 in in IN 10_1101-2021_01_07_425794 31 9 different different JJ 10_1101-2021_01_07_425794 31 10 studies study NNS 10_1101-2021_01_07_425794 31 11 makes make VBZ 10_1101-2021_01_07_425794 31 12 it -PRON- PRP 10_1101-2021_01_07_425794 31 13 difficult difficult JJ 10_1101-2021_01_07_425794 31 14 for for IN 10_1101-2021_01_07_425794 31 15 researchers researcher NNS 10_1101-2021_01_07_425794 31 16 to to TO 10_1101-2021_01_07_425794 31 17 reproduce reproduce VB 10_1101-2021_01_07_425794 31 18 the the DT 10_1101-2021_01_07_425794 31 19 findings finding NNS 10_1101-2021_01_07_425794 31 20 from from IN 10_1101-2021_01_07_425794 31 21 such such JJ 10_1101-2021_01_07_425794 31 22 studies study NNS 10_1101-2021_01_07_425794 31 23 . . . 10_1101-2021_01_07_425794 32 1 For for IN 10_1101-2021_01_07_425794 32 2 example example NN 10_1101-2021_01_07_425794 32 3 , , , 10_1101-2021_01_07_425794 32 4 large large JJ 10_1101-2021_01_07_425794 32 5 consortia consortium NNS 10_1101-2021_01_07_425794 32 6 such such JJ 10_1101-2021_01_07_425794 32 7 as as IN 10_1101-2021_01_07_425794 32 8 the the DT 10_1101-2021_01_07_425794 32 9 European European NNP 10_1101-2021_01_07_425794 32 10 Molecular Molecular NNP 10_1101-2021_01_07_425794 32 11 Biology Biology NNP 10_1101-2021_01_07_425794 32 12 Laboratory Laboratory NNP 10_1101-2021_01_07_425794 32 13 ( ( -LRB- 10_1101-2021_01_07_425794 32 14 EMBL EMBL NNP 10_1101-2021_01_07_425794 32 15 ) ) -RRB- 10_1101-2021_01_07_425794 32 16 use use VBP 10_1101-2021_01_07_425794 32 17 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 32 18 in in IN 10_1101-2021_01_07_425794 32 19 their -PRON- PRP$ 10_1101-2021_01_07_425794 32 20 studies study NNS 10_1101-2021_01_07_425794 32 21 while while IN 10_1101-2021_01_07_425794 32 22 the the DT 10_1101-2021_01_07_425794 32 23 National National NNP 10_1101-2021_01_07_425794 32 24 Centre Centre NNP 10_1101-2021_01_07_425794 32 25 for for IN 10_1101-2021_01_07_425794 32 26 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425794 32 27 Information Information NNP 10_1101-2021_01_07_425794 32 28 ( ( -LRB- 10_1101-2021_01_07_425794 32 29 NCBI NCBI NNP 10_1101-2021_01_07_425794 32 30 ) ) -RRB- 10_1101-2021_01_07_425794 32 31 tend tend VBP 10_1101-2021_01_07_425794 32 32 to to TO 10_1101-2021_01_07_425794 32 33 use use VB 10_1101-2021_01_07_425794 32 34 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 32 35 . . . 10_1101-2021_01_07_425794 33 1 Since since IN 10_1101-2021_01_07_425794 33 2 this this DT 10_1101-2021_01_07_425794 33 3 can can MD 10_1101-2021_01_07_425794 33 4 significantly significantly RB 10_1101-2021_01_07_425794 33 5 impact impact VB 10_1101-2021_01_07_425794 33 6 on on IN 10_1101-2021_01_07_425794 33 7 gene gene NN 10_1101-2021_01_07_425794 33 8 expression expression NN 10_1101-2021_01_07_425794 33 9 data datum NNS 10_1101-2021_01_07_425794 33 10 , , , 10_1101-2021_01_07_425794 33 11 there there EX 10_1101-2021_01_07_425794 33 12 is be VBZ 10_1101-2021_01_07_425794 33 13 a a DT 10_1101-2021_01_07_425794 33 14 need need NN 10_1101-2021_01_07_425794 33 15 to to TO 10_1101-2021_01_07_425794 33 16 develop develop VB 10_1101-2021_01_07_425794 33 17 a a DT 10_1101-2021_01_07_425794 33 18 comprehensive comprehensive JJ 10_1101-2021_01_07_425794 33 19 understanding understanding NN 10_1101-2021_01_07_425794 33 20 of of IN 10_1101-2021_01_07_425794 33 21 how how WRB 10_1101-2021_01_07_425794 33 22 these these DT 10_1101-2021_01_07_425794 33 23 differences difference NNS 10_1101-2021_01_07_425794 33 24 in in IN 10_1101-2021_01_07_425794 33 25 annotations annotation NNS 10_1101-2021_01_07_425794 33 26 impact impact VBP 10_1101-2021_01_07_425794 33 27 the the DT 10_1101-2021_01_07_425794 33 28 gene- gene- JJ 10_1101-2021_01_07_425794 33 29 level level NN 10_1101-2021_01_07_425794 33 30 expression expression NN 10_1101-2021_01_07_425794 33 31 quantification quantification NN 10_1101-2021_01_07_425794 33 32 . . . 10_1101-2021_01_07_425794 34 1 In in IN 10_1101-2021_01_07_425794 34 2 this this DT 10_1101-2021_01_07_425794 34 3 study study NN 10_1101-2021_01_07_425794 34 4 , , , 10_1101-2021_01_07_425794 34 5 we -PRON- PRP 10_1101-2021_01_07_425794 34 6 compared compare VBD 10_1101-2021_01_07_425794 34 7 three three CD 10_1101-2021_01_07_425794 34 8 human human JJ 10_1101-2021_01_07_425794 34 9 gene gene NN 10_1101-2021_01_07_425794 34 10 annotations annotation NNS 10_1101-2021_01_07_425794 34 11 , , , 10_1101-2021_01_07_425794 34 12 including include VBG 10_1101-2021_01_07_425794 34 13 a a DT 10_1101-2021_01_07_425794 34 14 recent recent JJ 10_1101-2021_01_07_425794 34 15 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 34 16 annotation annotation NN 10_1101-2021_01_07_425794 34 17 ( ( -LRB- 10_1101-2021_01_07_425794 34 18 released release VBN 10_1101-2021_01_07_425794 34 19 in in IN 10_1101-2021_01_07_425794 34 20 April April NNP 10_1101-2021_01_07_425794 34 21 2020 2020 CD 10_1101-2021_01_07_425794 34 22 ) ) -RRB- 10_1101-2021_01_07_425794 34 23 , , , 10_1101-2021_01_07_425794 34 24 a a DT 10_1101-2021_01_07_425794 34 25 recent recent JJ 10_1101-2021_01_07_425794 34 26 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 34 27 annotation annotation NN 10_1101-2021_01_07_425794 34 28 ( ( -LRB- 10_1101-2021_01_07_425794 34 29 released release VBN 10_1101-2021_01_07_425794 34 30 in in IN 10_1101-2021_01_07_425794 34 31 August August NNP 10_1101-2021_01_07_425794 34 32 2020 2020 CD 10_1101-2021_01_07_425794 34 33 ) ) -RRB- 10_1101-2021_01_07_425794 34 34 and and CC 10_1101-2021_01_07_425794 34 35 an an DT 10_1101-2021_01_07_425794 34 36 old old JJ 10_1101-2021_01_07_425794 34 37 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 34 38 annotation annotation NN 10_1101-2021_01_07_425794 34 39 ( ( -LRB- 10_1101-2021_01_07_425794 34 40 released release VBN 10_1101-2021_01_07_425794 34 41 in in IN 10_1101-2021_01_07_425794 34 42 April April NNP 10_1101-2021_01_07_425794 34 43 2015 2015 CD 10_1101-2021_01_07_425794 34 44 ) ) -RRB- 10_1101-2021_01_07_425794 34 45 , , , 10_1101-2021_01_07_425794 34 46 to to TO 10_1101-2021_01_07_425794 34 47 understand understand VB 10_1101-2021_01_07_425794 34 48 their -PRON- PRP$ 10_1101-2021_01_07_425794 34 49 impact impact NN 10_1101-2021_01_07_425794 34 50 on on IN 10_1101-2021_01_07_425794 34 51 gene gene NN 10_1101-2021_01_07_425794 34 52 - - HYPH 10_1101-2021_01_07_425794 34 53 level level NN 10_1101-2021_01_07_425794 34 54 expression expression NN 10_1101-2021_01_07_425794 34 55 quantification quantification NN 10_1101-2021_01_07_425794 34 56 in in IN 10_1101-2021_01_07_425794 34 57 an an DT 10_1101-2021_01_07_425794 34 58 RNA RNA NNP 10_1101-2021_01_07_425794 34 59 - - HYPH 10_1101-2021_01_07_425794 34 60 seq seq NN 10_1101-2021_01_07_425794 34 61 data datum NNS 10_1101-2021_01_07_425794 34 62 analysis analysis NN 10_1101-2021_01_07_425794 34 63 pipeline pipeline NN 10_1101-2021_01_07_425794 34 64 . . . 10_1101-2021_01_07_425794 35 1 Although although IN 10_1101-2021_01_07_425794 35 2 the the DT 10_1101-2021_01_07_425794 35 3 old old JJ 10_1101-2021_01_07_425794 35 4 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 35 5 annotation annotation NN 10_1101-2021_01_07_425794 35 6 is be VBZ 10_1101-2021_01_07_425794 35 7 not not RB 10_1101-2021_01_07_425794 35 8 available available JJ 10_1101-2021_01_07_425794 35 9 at at IN 10_1101-2021_01_07_425794 35 10 the the DT 10_1101-2021_01_07_425794 35 11 NCBI NCBI NNP 10_1101-2021_01_07_425794 35 12 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 35 13 database database NN 10_1101-2021_01_07_425794 35 14 anymore anymore RB 10_1101-2021_01_07_425794 35 15 , , , 10_1101-2021_01_07_425794 35 16 it -PRON- PRP 10_1101-2021_01_07_425794 35 17 has have VBZ 10_1101-2021_01_07_425794 35 18 been be VBN 10_1101-2021_01_07_425794 35 19 included include VBN 10_1101-2021_01_07_425794 35 20 as as IN 10_1101-2021_01_07_425794 35 21 part part NN 10_1101-2021_01_07_425794 35 22 of of IN 10_1101-2021_01_07_425794 35 23 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 35 24 , , , 10_1101-2021_01_07_425794 35 25 a a DT 10_1101-2021_01_07_425794 35 26 popular popular JJ 10_1101-2021_01_07_425794 35 27 RNA RNA NNP 10_1101-2021_01_07_425794 35 28 - - HYPH 10_1101-2021_01_07_425794 35 29 seq seq NN 10_1101-2021_01_07_425794 35 30 quantification quantification NN 10_1101-2021_01_07_425794 35 31 toolkit toolkit NNS 10_1101-2021_01_07_425794 35 32 , , , 10_1101-2021_01_07_425794 35 33 for for IN 10_1101-2021_01_07_425794 35 34 quantifying quantify VBG 10_1101-2021_01_07_425794 35 35 human human JJ 10_1101-2021_01_07_425794 35 36 RNA RNA NNP 10_1101-2021_01_07_425794 35 37 - - HYPH 10_1101-2021_01_07_425794 35 38 seq seq NN 10_1101-2021_01_07_425794 35 39 data datum NNS 10_1101-2021_01_07_425794 35 40 . . . 10_1101-2021_01_07_425794 36 1 We -PRON- PRP 10_1101-2021_01_07_425794 36 2 used use VBD 10_1101-2021_01_07_425794 36 3 a a DT 10_1101-2021_01_07_425794 36 4 benchmark benchmark JJ 10_1101-2021_01_07_425794 36 5 RNA RNA NNP 10_1101-2021_01_07_425794 36 6 - - HYPH 10_1101-2021_01_07_425794 36 7 seq seq NN 10_1101-2021_01_07_425794 36 8 dataset dataset NN 10_1101-2021_01_07_425794 36 9 generated generate VBN 10_1101-2021_01_07_425794 36 10 by by IN 10_1101-2021_01_07_425794 36 11 the the DT 10_1101-2021_01_07_425794 36 12 SEquencing SEquencing NNP 10_1101-2021_01_07_425794 36 13 Quality Quality NNP 10_1101-2021_01_07_425794 36 14 Control Control NNP 10_1101-2021_01_07_425794 36 15 ( ( -LRB- 10_1101-2021_01_07_425794 36 16 SEQC SEQC NNP 10_1101-2021_01_07_425794 36 17 / / SYM 10_1101-2021_01_07_425794 36 18 MAQC MAQC NNP 10_1101-2021_01_07_425794 36 19 III III NNP 10_1101-2021_01_07_425794 36 20 ) ) -RRB- 10_1101-2021_01_07_425794 36 21 consortium consortium NN 10_1101-2021_01_07_425794 36 22 for for IN 10_1101-2021_01_07_425794 36 23 this this DT 10_1101-2021_01_07_425794 36 24 evaluation evaluation NN 10_1101-2021_01_07_425794 36 25 . . . 10_1101-2021_01_07_425794 37 1 We -PRON- PRP 10_1101-2021_01_07_425794 37 2 show show VBP 10_1101-2021_01_07_425794 37 3 that that IN 10_1101-2021_01_07_425794 37 4 the the DT 10_1101-2021_01_07_425794 37 5 use use NN 10_1101-2021_01_07_425794 37 6 of of IN 10_1101-2021_01_07_425794 37 7 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 37 8 gene gene NN 10_1101-2021_01_07_425794 37 9 annotations annotation NNS 10_1101-2021_01_07_425794 37 10 led lead VBD 10_1101-2021_01_07_425794 37 11 to to IN 10_1101-2021_01_07_425794 37 12 better well JJR 10_1101-2021_01_07_425794 37 13 quantification quantification NN 10_1101-2021_01_07_425794 37 14 accuracy accuracy NN 10_1101-2021_01_07_425794 37 15 than than IN 10_1101-2021_01_07_425794 37 16 the the DT 10_1101-2021_01_07_425794 37 17 use use NN 10_1101-2021_01_07_425794 37 18 of of IN 10_1101-2021_01_07_425794 37 19 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 37 20 annotation annotation NN 10_1101-2021_01_07_425794 37 21 , , , 10_1101-2021_01_07_425794 37 22 based base VBN 10_1101-2021_01_07_425794 37 23 on on IN 10_1101-2021_01_07_425794 37 24 the the DT 10_1101-2021_01_07_425794 37 25 correlation correlation NN 10_1101-2021_01_07_425794 37 26 with with IN 10_1101-2021_01_07_425794 37 27 ground ground NN 10_1101-2021_01_07_425794 37 28 truths truth NNS 10_1101-2021_01_07_425794 37 29 including include VBG 10_1101-2021_01_07_425794 37 30 expression expression NN 10_1101-2021_01_07_425794 37 31 data datum NNS 10_1101-2021_01_07_425794 37 32 from from IN 10_1101-2021_01_07_425794 37 33 > > XX 10_1101-2021_01_07_425794 37 34 800 800 CD 10_1101-2021_01_07_425794 37 35 real real JJ 10_1101-2021_01_07_425794 37 36 - - HYPH 10_1101-2021_01_07_425794 37 37 time time NN 10_1101-2021_01_07_425794 37 38 PCR PCR NNP 10_1101-2021_01_07_425794 37 39 validated validate VBN 10_1101-2021_01_07_425794 37 40 genes gene NNS 10_1101-2021_01_07_425794 37 41 , , , 10_1101-2021_01_07_425794 37 42 known know VBN 10_1101-2021_01_07_425794 37 43 genome genome NN 10_1101-2021_01_07_425794 37 44 - - HYPH 10_1101-2021_01_07_425794 37 45 wide wide JJ 10_1101-2021_01_07_425794 37 46 titration titration NN 10_1101-2021_01_07_425794 37 47 ratios ratio NNS 10_1101-2021_01_07_425794 37 48 of of IN 10_1101-2021_01_07_425794 37 49 gene gene NN 10_1101-2021_01_07_425794 37 50 expression expression NN 10_1101-2021_01_07_425794 37 51 and and CC 10_1101-2021_01_07_425794 37 52 microarray microarray NNP 10_1101-2021_01_07_425794 37 53 gene gene NN 10_1101-2021_01_07_425794 37 54 expression expression NN 10_1101-2021_01_07_425794 37 55 data datum NNS 10_1101-2021_01_07_425794 37 56 . . . 10_1101-2021_01_07_425794 38 1 We -PRON- PRP 10_1101-2021_01_07_425794 38 2 also also RB 10_1101-2021_01_07_425794 38 3 show show VBP 10_1101-2021_01_07_425794 38 4 that that IN 10_1101-2021_01_07_425794 38 5 the the DT 10_1101-2021_01_07_425794 38 6 older old JJR 10_1101-2021_01_07_425794 38 7 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 38 8 annotation annotation NN 10_1101-2021_01_07_425794 38 9 yielded yield VBD 10_1101-2021_01_07_425794 38 10 higher high JJR 10_1101-2021_01_07_425794 38 11 quantification quantification NN 10_1101-2021_01_07_425794 38 12 accuracy accuracy NN 10_1101-2021_01_07_425794 38 13 than than IN 10_1101-2021_01_07_425794 38 14 the the DT 10_1101-2021_01_07_425794 38 15 recent recent JJ 10_1101-2021_01_07_425794 38 16 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 38 17 annotation annotation NN 10_1101-2021_01_07_425794 38 18 in in IN 10_1101-2021_01_07_425794 38 19 our -PRON- PRP$ 10_1101-2021_01_07_425794 38 20 evaluations evaluation NNS 10_1101-2021_01_07_425794 38 21 , , , 10_1101-2021_01_07_425794 38 22 suggesting suggest VBG 10_1101-2021_01_07_425794 38 23 that that IN 10_1101-2021_01_07_425794 38 24 the the DT 10_1101-2021_01_07_425794 38 25 recent recent JJ 10_1101-2021_01_07_425794 38 26 expansion expansion NN 10_1101-2021_01_07_425794 38 27 and and CC 10_1101-2021_01_07_425794 38 28 changes change NNS 10_1101-2021_01_07_425794 38 29 made make VBN 10_1101-2021_01_07_425794 38 30 to to IN 10_1101-2021_01_07_425794 38 31 the the DT 10_1101-2021_01_07_425794 38 32 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 38 33 annotation annotation NN 10_1101-2021_01_07_425794 38 34 have have VBP 10_1101-2021_01_07_425794 38 35 led lead VBN 10_1101-2021_01_07_425794 38 36 to to IN 10_1101-2021_01_07_425794 38 37 a a DT 10_1101-2021_01_07_425794 38 38 decline decline NN 10_1101-2021_01_07_425794 38 39 in in IN 10_1101-2021_01_07_425794 38 40 annotation annotation NN 10_1101-2021_01_07_425794 38 41 accuracy accuracy NN 10_1101-2021_01_07_425794 38 42 resulting result VBG 10_1101-2021_01_07_425794 38 43 in in IN 10_1101-2021_01_07_425794 38 44 less less RBR 10_1101-2021_01_07_425794 38 45 accurate accurate JJ 10_1101-2021_01_07_425794 38 46 quantification quantification NN 10_1101-2021_01_07_425794 38 47 result result NN 10_1101-2021_01_07_425794 38 48 . . . 10_1101-2021_01_07_425794 39 1 Furthermore furthermore RB 10_1101-2021_01_07_425794 39 2 , , , 10_1101-2021_01_07_425794 39 3 we -PRON- PRP 10_1101-2021_01_07_425794 39 4 investigated investigate VBD 10_1101-2021_01_07_425794 39 5 if if IN 10_1101-2021_01_07_425794 39 6 any any DT 10_1101-2021_01_07_425794 39 7 normalization normalization NN 10_1101-2021_01_07_425794 39 8 method method NN 10_1101-2021_01_07_425794 39 9 can can MD 10_1101-2021_01_07_425794 39 10 mitigate mitigate VB 10_1101-2021_01_07_425794 39 11 the the DT 10_1101-2021_01_07_425794 39 12 differences difference NNS 10_1101-2021_01_07_425794 39 13 in in IN 10_1101-2021_01_07_425794 39 14 quantification quantification NN 10_1101-2021_01_07_425794 39 15 results result NNS 10_1101-2021_01_07_425794 39 16 caused cause VBN 10_1101-2021_01_07_425794 39 17 by by IN 10_1101-2021_01_07_425794 39 18 the the DT 10_1101-2021_01_07_425794 39 19 annotation annotation NN 10_1101-2021_01_07_425794 39 20 differences difference NNS 10_1101-2021_01_07_425794 39 21 . . . 10_1101-2021_01_07_425794 40 1 Our -PRON- PRP$ 10_1101-2021_01_07_425794 40 2 results result NNS 10_1101-2021_01_07_425794 40 3 show show VBP 10_1101-2021_01_07_425794 40 4 that that IN 10_1101-2021_01_07_425794 40 5 the the DT 10_1101-2021_01_07_425794 40 6 quantification quantification NN 10_1101-2021_01_07_425794 40 7 differences difference NNS 10_1101-2021_01_07_425794 40 8 remained remain VBD 10_1101-2021_01_07_425794 40 9 almost almost RB 10_1101-2021_01_07_425794 40 10 the the DT 10_1101-2021_01_07_425794 40 11 same same JJ 10_1101-2021_01_07_425794 40 12 no no RB 10_1101-2021_01_07_425794 40 13 matter matter RB 10_1101-2021_01_07_425794 40 14 how how WRB 10_1101-2021_01_07_425794 40 15 the the DT 10_1101-2021_01_07_425794 40 16 RNA RNA NNP 10_1101-2021_01_07_425794 40 17 - - HYPH 10_1101-2021_01_07_425794 40 18 seq seq NN 10_1101-2021_01_07_425794 40 19 data datum NNS 10_1101-2021_01_07_425794 40 20 were be VBD 10_1101-2021_01_07_425794 40 21 normalized normalized JJ 10_1101-2021_01_07_425794 40 22 . . . 10_1101-2021_01_07_425794 41 1 2 2 CD 10_1101-2021_01_07_425794 41 2 MATERIALS material NNS 10_1101-2021_01_07_425794 41 3 AND and CC 10_1101-2021_01_07_425794 41 4 METHODS methods JJ 10_1101-2021_01_07_425794 41 5 2.1 2.1 CD 10_1101-2021_01_07_425794 41 6 SEQC SEQC NNP 10_1101-2021_01_07_425794 41 7 / / SYM 10_1101-2021_01_07_425794 41 8 MAQC MAQC NNP 10_1101-2021_01_07_425794 41 9 data datum NNS 10_1101-2021_01_07_425794 41 10 The the DT 10_1101-2021_01_07_425794 41 11 RNA RNA NNP 10_1101-2021_01_07_425794 41 12 - - HYPH 10_1101-2021_01_07_425794 41 13 seq seq NN 10_1101-2021_01_07_425794 41 14 data datum NNS 10_1101-2021_01_07_425794 41 15 used use VBN 10_1101-2021_01_07_425794 41 16 for for IN 10_1101-2021_01_07_425794 41 17 evaluation evaluation NN 10_1101-2021_01_07_425794 41 18 in in IN 10_1101-2021_01_07_425794 41 19 this this DT 10_1101-2021_01_07_425794 41 20 study study NN 10_1101-2021_01_07_425794 41 21 are be VBP 10_1101-2021_01_07_425794 41 22 a a DT 10_1101-2021_01_07_425794 41 23 benchmark benchmark JJ 10_1101-2021_01_07_425794 41 24 dataset dataset NN 10_1101-2021_01_07_425794 41 25 generated generate VBN 10_1101-2021_01_07_425794 41 26 by by IN 10_1101-2021_01_07_425794 41 27 the the DT 10_1101-2021_01_07_425794 41 28 Sequencing Sequencing NNP 10_1101-2021_01_07_425794 41 29 Quality Quality NNP 10_1101-2021_01_07_425794 41 30 Control Control NNP 10_1101-2021_01_07_425794 41 31 ( ( -LRB- 10_1101-2021_01_07_425794 41 32 SEQC SEQC NNP 10_1101-2021_01_07_425794 41 33 ) ) -RRB- 10_1101-2021_01_07_425794 41 34 project project NN 10_1101-2021_01_07_425794 41 35 [ [ -LRB- 10_1101-2021_01_07_425794 41 36 1 1 CD 10_1101-2021_01_07_425794 41 37 ] ] -RRB- 10_1101-2021_01_07_425794 41 38 , , , 10_1101-2021_01_07_425794 41 39 the the DT 10_1101-2021_01_07_425794 41 40 third third JJ 10_1101-2021_01_07_425794 41 41 stage stage NN 10_1101-2021_01_07_425794 41 42 of of IN 10_1101-2021_01_07_425794 41 43 the the DT 10_1101-2021_01_07_425794 41 44 MicroArray MicroArray NNP 10_1101-2021_01_07_425794 41 45 Quality Quality NNP 10_1101-2021_01_07_425794 41 46 Control Control NNP 10_1101-2021_01_07_425794 41 47 ( ( -LRB- 10_1101-2021_01_07_425794 41 48 MAQC MAQC NNP 10_1101-2021_01_07_425794 41 49 ) ) -RRB- 10_1101-2021_01_07_425794 41 50 study study NN 10_1101-2021_01_07_425794 41 51 [ [ -LRB- 10_1101-2021_01_07_425794 41 52 14 14 CD 10_1101-2021_01_07_425794 41 53 , , , 10_1101-2021_01_07_425794 41 54 15 15 CD 10_1101-2021_01_07_425794 41 55 ] ] -RRB- 10_1101-2021_01_07_425794 41 56 . . . 10_1101-2021_01_07_425794 42 1 The the DT 10_1101-2021_01_07_425794 42 2 SEQC SEQC NNP 10_1101-2021_01_07_425794 42 3 dataset dataset NN 10_1101-2021_01_07_425794 42 4 includes include VBZ 10_1101-2021_01_07_425794 42 5 the the DT 10_1101-2021_01_07_425794 42 6 Universal Universal NNP 10_1101-2021_01_07_425794 42 7 3 3 CD 10_1101-2021_01_07_425794 42 8 .CC .CC : 10_1101-2021_01_07_425794 42 9 - - HYPH 10_1101-2021_01_07_425794 42 10 BY by IN 10_1101-2021_01_07_425794 42 11 - - HYPH 10_1101-2021_01_07_425794 42 12 NC NC NNP 10_1101-2021_01_07_425794 42 13 - - HYPH 10_1101-2021_01_07_425794 42 14 ND ND NNP 10_1101-2021_01_07_425794 42 15 4.0 4.0 CD 10_1101-2021_01_07_425794 42 16 International international JJ 10_1101-2021_01_07_425794 42 17 licensemade licensemade NN 10_1101-2021_01_07_425794 42 18 available available JJ 10_1101-2021_01_07_425794 42 19 under under IN 10_1101-2021_01_07_425794 42 20 a a DT 10_1101-2021_01_07_425794 42 21 ( ( -LRB- 10_1101-2021_01_07_425794 42 22 which which WDT 10_1101-2021_01_07_425794 42 23 was be VBD 10_1101-2021_01_07_425794 42 24 not not RB 10_1101-2021_01_07_425794 42 25 certified certify VBN 10_1101-2021_01_07_425794 42 26 by by IN 10_1101-2021_01_07_425794 42 27 peer peer NN 10_1101-2021_01_07_425794 42 28 review review NN 10_1101-2021_01_07_425794 42 29 ) ) -RRB- 10_1101-2021_01_07_425794 42 30 is be VBZ 10_1101-2021_01_07_425794 42 31 the the DT 10_1101-2021_01_07_425794 42 32 author author NN 10_1101-2021_01_07_425794 42 33 / / SYM 10_1101-2021_01_07_425794 42 34 funder funder NN 10_1101-2021_01_07_425794 42 35 , , , 10_1101-2021_01_07_425794 42 36 who who WP 10_1101-2021_01_07_425794 42 37 has have VBZ 10_1101-2021_01_07_425794 42 38 granted grant VBN 10_1101-2021_01_07_425794 42 39 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 42 40 a a DT 10_1101-2021_01_07_425794 42 41 license license NN 10_1101-2021_01_07_425794 42 42 to to TO 10_1101-2021_01_07_425794 42 43 display display VB 10_1101-2021_01_07_425794 42 44 the the DT 10_1101-2021_01_07_425794 42 45 preprint preprint NN 10_1101-2021_01_07_425794 42 46 in in IN 10_1101-2021_01_07_425794 42 47 perpetuity perpetuity NN 10_1101-2021_01_07_425794 42 48 . . . 10_1101-2021_01_07_425794 43 1 It -PRON- PRP 10_1101-2021_01_07_425794 43 2 is be VBZ 10_1101-2021_01_07_425794 43 3 The the DT 10_1101-2021_01_07_425794 43 4 copyright copyright NN 10_1101-2021_01_07_425794 43 5 holder holder NN 10_1101-2021_01_07_425794 43 6 for for IN 10_1101-2021_01_07_425794 43 7 this this DT 10_1101-2021_01_07_425794 43 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 43 9 version version NN 10_1101-2021_01_07_425794 43 10 posted post VBD 10_1101-2021_01_07_425794 43 11 January January NNP 10_1101-2021_01_07_425794 43 12 8 8 CD 10_1101-2021_01_07_425794 43 13 , , , 10_1101-2021_01_07_425794 43 14 2021 2021 CD 10_1101-2021_01_07_425794 43 15 . . . 10_1101-2021_01_07_425794 43 16 ; ; : 10_1101-2021_01_07_425794 43 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 43 18 : : : 10_1101-2021_01_07_425794 43 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 43 20 preprint preprint JJ 10_1101-2021_01_07_425794 43 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 43 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NNP 10_1101-2021_01_07_425794 43 23 Human Human NNP 10_1101-2021_01_07_425794 43 24 Reference Reference NNP 10_1101-2021_01_07_425794 43 25 RNA RNA NNP 10_1101-2021_01_07_425794 43 26 ( ( -LRB- 10_1101-2021_01_07_425794 43 27 UHRR UHRR NNP 10_1101-2021_01_07_425794 43 28 ) ) -RRB- 10_1101-2021_01_07_425794 43 29 as as IN 10_1101-2021_01_07_425794 43 30 sample sample NN 10_1101-2021_01_07_425794 43 31 A A NNP 10_1101-2021_01_07_425794 43 32 and and CC 10_1101-2021_01_07_425794 43 33 the the DT 10_1101-2021_01_07_425794 43 34 Human Human NNP 10_1101-2021_01_07_425794 43 35 Brain Brain NNP 10_1101-2021_01_07_425794 43 36 Reference Reference NNP 10_1101-2021_01_07_425794 43 37 RNA RNA NNP 10_1101-2021_01_07_425794 43 38 ( ( -LRB- 10_1101-2021_01_07_425794 43 39 HBRR HBRR NNP 10_1101-2021_01_07_425794 43 40 ) ) -RRB- 10_1101-2021_01_07_425794 43 41 as as IN 10_1101-2021_01_07_425794 43 42 sample sample NNP 10_1101-2021_01_07_425794 43 43 B. B. NNP 10_1101-2021_01_07_425794 44 1 It -PRON- PRP 10_1101-2021_01_07_425794 44 2 also also RB 10_1101-2021_01_07_425794 44 3 includes include VBZ 10_1101-2021_01_07_425794 44 4 two two CD 10_1101-2021_01_07_425794 44 5 other other JJ 10_1101-2021_01_07_425794 44 6 samples sample NNS 10_1101-2021_01_07_425794 44 7 C c NN 10_1101-2021_01_07_425794 44 8 and and CC 10_1101-2021_01_07_425794 44 9 D d NN 10_1101-2021_01_07_425794 44 10 , , , 10_1101-2021_01_07_425794 44 11 which which WDT 10_1101-2021_01_07_425794 44 12 are be VBP 10_1101-2021_01_07_425794 44 13 combination combination NN 10_1101-2021_01_07_425794 44 14 of of IN 10_1101-2021_01_07_425794 44 15 A a NN 10_1101-2021_01_07_425794 44 16 and and CC 10_1101-2021_01_07_425794 44 17 B b NN 10_1101-2021_01_07_425794 44 18 mixed mix VBN 10_1101-2021_01_07_425794 44 19 in in IN 10_1101-2021_01_07_425794 44 20 the the DT 10_1101-2021_01_07_425794 44 21 ratios ratio NNS 10_1101-2021_01_07_425794 44 22 of of IN 10_1101-2021_01_07_425794 44 23 3:1 3:1 CD 10_1101-2021_01_07_425794 44 24 in in IN 10_1101-2021_01_07_425794 44 25 C C NNP 10_1101-2021_01_07_425794 44 26 and and CC 10_1101-2021_01_07_425794 44 27 1:3 1:3 CD 10_1101-2021_01_07_425794 44 28 in in IN 10_1101-2021_01_07_425794 44 29 D d NN 10_1101-2021_01_07_425794 44 30 respectively respectively RB 10_1101-2021_01_07_425794 44 31 . . . 10_1101-2021_01_07_425794 45 1 The the DT 10_1101-2021_01_07_425794 45 2 samples sample NNS 10_1101-2021_01_07_425794 45 3 were be VBD 10_1101-2021_01_07_425794 45 4 sequenced sequence VBN 10_1101-2021_01_07_425794 45 5 in in IN 10_1101-2021_01_07_425794 45 6 four four CD 10_1101-2021_01_07_425794 45 7 replicate replicate NN 10_1101-2021_01_07_425794 45 8 paired pair VBN 10_1101-2021_01_07_425794 45 9 - - HYPH 10_1101-2021_01_07_425794 45 10 end end NN 10_1101-2021_01_07_425794 45 11 libraries library NNS 10_1101-2021_01_07_425794 45 12 using use VBG 10_1101-2021_01_07_425794 45 13 an an DT 10_1101-2021_01_07_425794 45 14 Illumina Illumina NNP 10_1101-2021_01_07_425794 45 15 HiSeq HiSeq NNP 10_1101-2021_01_07_425794 45 16 2000 2000 CD 10_1101-2021_01_07_425794 45 17 sequencer sequencer NN 10_1101-2021_01_07_425794 45 18 at at IN 10_1101-2021_01_07_425794 45 19 the the DT 10_1101-2021_01_07_425794 45 20 Australian Australian NNP 10_1101-2021_01_07_425794 45 21 Genomics Genomics NNP 10_1101-2021_01_07_425794 45 22 Research Research NNP 10_1101-2021_01_07_425794 45 23 Facility Facility NNP 10_1101-2021_01_07_425794 45 24 ( ( -LRB- 10_1101-2021_01_07_425794 45 25 AGRF AGRF NNP 10_1101-2021_01_07_425794 45 26 ) ) -RRB- 10_1101-2021_01_07_425794 45 27 . . . 10_1101-2021_01_07_425794 46 1 Each each DT 10_1101-2021_01_07_425794 46 2 library library NN 10_1101-2021_01_07_425794 46 3 contains contain VBZ 10_1101-2021_01_07_425794 46 4 ∼20 ∼20 NNP 10_1101-2021_01_07_425794 46 5 million million CD 10_1101-2021_01_07_425794 46 6 100bp 100bp NNP 10_1101-2021_01_07_425794 46 7 read read VB 10_1101-2021_01_07_425794 46 8 pairs pair NNS 10_1101-2021_01_07_425794 46 9 . . . 10_1101-2021_01_07_425794 47 1 A a DT 10_1101-2021_01_07_425794 47 2 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 47 3 real real JJ 10_1101-2021_01_07_425794 47 4 - - HYPH 10_1101-2021_01_07_425794 47 5 time time NN 10_1101-2021_01_07_425794 47 6 polymerase polymerase NN 10_1101-2021_01_07_425794 47 7 chain chain NN 10_1101-2021_01_07_425794 47 8 reaction reaction NN 10_1101-2021_01_07_425794 47 9 ( ( -LRB- 10_1101-2021_01_07_425794 47 10 RT RT NNP 10_1101-2021_01_07_425794 47 11 - - HYPH 10_1101-2021_01_07_425794 47 12 PCR PCR NNP 10_1101-2021_01_07_425794 47 13 ) ) -RRB- 10_1101-2021_01_07_425794 47 14 dataset dataset VBD 10_1101-2021_01_07_425794 47 15 with with IN 10_1101-2021_01_07_425794 47 16 expression expression NN 10_1101-2021_01_07_425794 47 17 values value NNS 10_1101-2021_01_07_425794 47 18 measured measure VBN 10_1101-2021_01_07_425794 47 19 for for IN 10_1101-2021_01_07_425794 47 20 over over IN 10_1101-2021_01_07_425794 47 21 1,000 1,000 CD 10_1101-2021_01_07_425794 47 22 genes gene NNS 10_1101-2021_01_07_425794 47 23 , , , 10_1101-2021_01_07_425794 47 24 which which WDT 10_1101-2021_01_07_425794 47 25 was be VBD 10_1101-2021_01_07_425794 47 26 generated generate VBN 10_1101-2021_01_07_425794 47 27 in in IN 10_1101-2021_01_07_425794 47 28 the the DT 10_1101-2021_01_07_425794 47 29 MAQC MAQC NNP 10_1101-2021_01_07_425794 47 30 - - , 10_1101-2021_01_07_425794 47 31 I -PRON- PRP 10_1101-2021_01_07_425794 47 32 study study VBP 10_1101-2021_01_07_425794 47 33 [ [ -LRB- 10_1101-2021_01_07_425794 47 34 15 15 CD 10_1101-2021_01_07_425794 47 35 ] ] -RRB- 10_1101-2021_01_07_425794 47 36 , , , 10_1101-2021_01_07_425794 47 37 was be VBD 10_1101-2021_01_07_425794 47 38 used use VBN 10_1101-2021_01_07_425794 47 39 to to TO 10_1101-2021_01_07_425794 47 40 validate validate VB 10_1101-2021_01_07_425794 47 41 the the DT 10_1101-2021_01_07_425794 47 42 expression expression NN 10_1101-2021_01_07_425794 47 43 of of IN 10_1101-2021_01_07_425794 47 44 the the DT 10_1101-2021_01_07_425794 47 45 RNA RNA NNP 10_1101-2021_01_07_425794 47 46 - - HYPH 10_1101-2021_01_07_425794 47 47 seq seq NN 10_1101-2021_01_07_425794 47 48 data datum NNS 10_1101-2021_01_07_425794 47 49 in in IN 10_1101-2021_01_07_425794 47 50 this this DT 10_1101-2021_01_07_425794 47 51 study study NN 10_1101-2021_01_07_425794 47 52 . . . 10_1101-2021_01_07_425794 48 1 The the DT 10_1101-2021_01_07_425794 48 2 expression expression NN 10_1101-2021_01_07_425794 48 3 values value NNS 10_1101-2021_01_07_425794 48 4 were be VBD 10_1101-2021_01_07_425794 48 5 measured measure VBN 10_1101-2021_01_07_425794 48 6 for for IN 10_1101-2021_01_07_425794 48 7 both both CC 10_1101-2021_01_07_425794 48 8 the the DT 10_1101-2021_01_07_425794 48 9 UHRR UHRR NNP 10_1101-2021_01_07_425794 48 10 and and CC 10_1101-2021_01_07_425794 48 11 HBRR HBRR NNP 10_1101-2021_01_07_425794 48 12 samples sample NNS 10_1101-2021_01_07_425794 48 13 together together RB 10_1101-2021_01_07_425794 48 14 with with IN 10_1101-2021_01_07_425794 48 15 their -PRON- PRP$ 10_1101-2021_01_07_425794 48 16 respec- respec- NN 10_1101-2021_01_07_425794 48 17 tive tive JJ 10_1101-2021_01_07_425794 48 18 combinations combination NNS 10_1101-2021_01_07_425794 48 19 . . . 10_1101-2021_01_07_425794 49 1 Around around IN 10_1101-2021_01_07_425794 49 2 800–900 800–900 CD 10_1101-2021_01_07_425794 49 3 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 49 4 RT RT NNP 10_1101-2021_01_07_425794 49 5 - - HYPH 10_1101-2021_01_07_425794 49 6 PCR PCR NNP 10_1101-2021_01_07_425794 49 7 genes gene NNS 10_1101-2021_01_07_425794 49 8 , , , 10_1101-2021_01_07_425794 49 9 which which WDT 10_1101-2021_01_07_425794 49 10 had have VBD 10_1101-2021_01_07_425794 49 11 matching match VBG 10_1101-2021_01_07_425794 49 12 gene gene NN 10_1101-2021_01_07_425794 49 13 identifiers identifier NNS 10_1101-2021_01_07_425794 49 14 with with IN 10_1101-2021_01_07_425794 49 15 expressed express VBN 10_1101-2021_01_07_425794 49 16 RNA RNA NNP 10_1101-2021_01_07_425794 49 17 - - HYPH 10_1101-2021_01_07_425794 49 18 seq seq NN 10_1101-2021_01_07_425794 49 19 genes gene NNS 10_1101-2021_01_07_425794 49 20 from from IN 10_1101-2021_01_07_425794 49 21 different different JJ 10_1101-2021_01_07_425794 49 22 annotations annotation NNS 10_1101-2021_01_07_425794 49 23 , , , 10_1101-2021_01_07_425794 49 24 were be VBD 10_1101-2021_01_07_425794 49 25 included include VBN 10_1101-2021_01_07_425794 49 26 for for IN 10_1101-2021_01_07_425794 49 27 assessing assess VBG 10_1101-2021_01_07_425794 49 28 the the DT 10_1101-2021_01_07_425794 49 29 accuracy accuracy NN 10_1101-2021_01_07_425794 49 30 of of IN 10_1101-2021_01_07_425794 49 31 RNA RNA NNP 10_1101-2021_01_07_425794 49 32 - - HYPH 10_1101-2021_01_07_425794 49 33 seq seq NN 10_1101-2021_01_07_425794 49 34 quantification quantification NN 10_1101-2021_01_07_425794 49 35 . . . 10_1101-2021_01_07_425794 50 1 In in IN 10_1101-2021_01_07_425794 50 2 addition addition NN 10_1101-2021_01_07_425794 50 3 , , , 10_1101-2021_01_07_425794 50 4 microarray microarray JJ 10_1101-2021_01_07_425794 50 5 data datum NNS 10_1101-2021_01_07_425794 50 6 generated generate VBN 10_1101-2021_01_07_425794 50 7 in in IN 10_1101-2021_01_07_425794 50 8 the the DT 10_1101-2021_01_07_425794 50 9 MAQC MAQC NNP 10_1101-2021_01_07_425794 50 10 - - : 10_1101-2021_01_07_425794 50 11 I -PRON- PRP 10_1101-2021_01_07_425794 50 12 study study VBP 10_1101-2021_01_07_425794 50 13 with with IN 10_1101-2021_01_07_425794 50 14 samples sample NNS 10_1101-2021_01_07_425794 50 15 A A NNP 10_1101-2021_01_07_425794 50 16 to to IN 10_1101-2021_01_07_425794 50 17 D d NN 10_1101-2021_01_07_425794 50 18 hybridized hybridize VBN 10_1101-2021_01_07_425794 50 19 to to IN 10_1101-2021_01_07_425794 50 20 the the DT 10_1101-2021_01_07_425794 50 21 Illumina Illumina NNP 10_1101-2021_01_07_425794 50 22 Human-6 Human-6 NNP 10_1101-2021_01_07_425794 50 23 Bead- Bead- NNP 10_1101-2021_01_07_425794 50 24 Chip Chip NNP 10_1101-2021_01_07_425794 50 25 microarrays microarray NNS 10_1101-2021_01_07_425794 50 26 were be VBD 10_1101-2021_01_07_425794 50 27 also also RB 10_1101-2021_01_07_425794 50 28 used use VBN 10_1101-2021_01_07_425794 50 29 in in IN 10_1101-2021_01_07_425794 50 30 the the DT 10_1101-2021_01_07_425794 50 31 assessment assessment NN 10_1101-2021_01_07_425794 50 32 . . . 10_1101-2021_01_07_425794 51 1 The the DT 10_1101-2021_01_07_425794 51 2 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 51 3 RT RT NNP 10_1101-2021_01_07_425794 51 4 - - HYPH 10_1101-2021_01_07_425794 51 5 PCR PCR NNP 10_1101-2021_01_07_425794 51 6 and and CC 10_1101-2021_01_07_425794 51 7 Illumina Illumina NNP 10_1101-2021_01_07_425794 51 8 microarray microarray JJ 10_1101-2021_01_07_425794 51 9 datasets dataset NNS 10_1101-2021_01_07_425794 51 10 are be VBP 10_1101-2021_01_07_425794 51 11 available available JJ 10_1101-2021_01_07_425794 51 12 as as IN 10_1101-2021_01_07_425794 51 13 part part NN 10_1101-2021_01_07_425794 51 14 of of IN 10_1101-2021_01_07_425794 51 15 the the DT 10_1101-2021_01_07_425794 51 16 Bioconductor Bioconductor NNP 10_1101-2021_01_07_425794 51 17 package package NN 10_1101-2021_01_07_425794 51 18 ‘ ' `` 10_1101-2021_01_07_425794 51 19 seqc seqc NN 10_1101-2021_01_07_425794 51 20 ’ ' '' 10_1101-2021_01_07_425794 51 21 [ [ -LRB- 10_1101-2021_01_07_425794 51 22 16 16 CD 10_1101-2021_01_07_425794 51 23 ] ] -RRB- 10_1101-2021_01_07_425794 51 24 . . . 10_1101-2021_01_07_425794 52 1 2.2 2.2 CD 10_1101-2021_01_07_425794 52 2 Annotations Annotations NNPS 10_1101-2021_01_07_425794 52 3 used use VBD 10_1101-2021_01_07_425794 52 4 Three three CD 10_1101-2021_01_07_425794 52 5 human human JJ 10_1101-2021_01_07_425794 52 6 gene gene NN 10_1101-2021_01_07_425794 52 7 annotations annotation NNS 10_1101-2021_01_07_425794 52 8 were be VBD 10_1101-2021_01_07_425794 52 9 included include VBN 10_1101-2021_01_07_425794 52 10 in in IN 10_1101-2021_01_07_425794 52 11 this this DT 10_1101-2021_01_07_425794 52 12 study study NN 10_1101-2021_01_07_425794 52 13 , , , 10_1101-2021_01_07_425794 52 14 including include VBG 10_1101-2021_01_07_425794 52 15 a a DT 10_1101-2021_01_07_425794 52 16 recent recent JJ 10_1101-2021_01_07_425794 52 17 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 52 18 annotation annotation NN 10_1101-2021_01_07_425794 52 19 , , , 10_1101-2021_01_07_425794 52 20 a a DT 10_1101-2021_01_07_425794 52 21 recent recent JJ 10_1101-2021_01_07_425794 52 22 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 52 23 annotation annotation NN 10_1101-2021_01_07_425794 52 24 and and CC 10_1101-2021_01_07_425794 52 25 an an DT 10_1101-2021_01_07_425794 52 26 old old JJ 10_1101-2021_01_07_425794 52 27 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 52 28 annotation annotation NN 10_1101-2021_01_07_425794 52 29 . . . 10_1101-2021_01_07_425794 53 1 All all PDT 10_1101-2021_01_07_425794 53 2 these these DT 10_1101-2021_01_07_425794 53 3 anno- anno- JJ 10_1101-2021_01_07_425794 53 4 tations tation NNS 10_1101-2021_01_07_425794 53 5 were be VBD 10_1101-2021_01_07_425794 53 6 generated generate VBN 10_1101-2021_01_07_425794 53 7 based base VBN 10_1101-2021_01_07_425794 53 8 on on IN 10_1101-2021_01_07_425794 53 9 the the DT 10_1101-2021_01_07_425794 53 10 human human JJ 10_1101-2021_01_07_425794 53 11 reference reference NN 10_1101-2021_01_07_425794 53 12 genome genome NNP 10_1101-2021_01_07_425794 53 13 GRCh38 GRCh38 NNP 10_1101-2021_01_07_425794 53 14 / / SYM 10_1101-2021_01_07_425794 53 15 hg38 hg38 NNP 10_1101-2021_01_07_425794 53 16 . . . 10_1101-2021_01_07_425794 54 1 The the DT 10_1101-2021_01_07_425794 54 2 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 54 3 gene gene NN 10_1101-2021_01_07_425794 54 4 annotation annotation NN 10_1101-2021_01_07_425794 54 5 used use VBN 10_1101-2021_01_07_425794 54 6 in in IN 10_1101-2021_01_07_425794 54 7 this this DT 10_1101-2021_01_07_425794 54 8 study study NN 10_1101-2021_01_07_425794 54 9 was be VBD 10_1101-2021_01_07_425794 54 10 generated generate VBN 10_1101-2021_01_07_425794 54 11 in in IN 10_1101-2021_01_07_425794 54 12 April April NNP 10_1101-2021_01_07_425794 54 13 2020 2020 CD 10_1101-2021_01_07_425794 54 14 . . . 10_1101-2021_01_07_425794 55 1 Its -PRON- PRP$ 10_1101-2021_01_07_425794 55 2 ver- ver- JJ 10_1101-2021_01_07_425794 55 3 sion sion NN 10_1101-2021_01_07_425794 55 4 number number NN 10_1101-2021_01_07_425794 55 5 is be VBZ 10_1101-2021_01_07_425794 55 6 100 100 CD 10_1101-2021_01_07_425794 55 7 . . . 10_1101-2021_01_07_425794 56 1 It -PRON- PRP 10_1101-2021_01_07_425794 56 2 was be VBD 10_1101-2021_01_07_425794 56 3 downloaded download VBN 10_1101-2021_01_07_425794 56 4 from from IN 10_1101-2021_01_07_425794 56 5 ftp://ftp.ensembl.org/pub/release-100/ ftp://ftp.ensembl.org/pub/release-100/ NNP 10_1101-2021_01_07_425794 56 6 gtf gtf NN 10_1101-2021_01_07_425794 56 7 / / SYM 10_1101-2021_01_07_425794 56 8 homo_sapiens homo_sapien NNS 10_1101-2021_01_07_425794 56 9 / / SYM 10_1101-2021_01_07_425794 56 10 Homo_sapiens homo_sapien NNS 10_1101-2021_01_07_425794 56 11 . . . 10_1101-2021_01_07_425794 56 12 GRCh38.100.gtf.gz grch38.100.gtf.gz ADD 10_1101-2021_01_07_425794 56 13 . . . 10_1101-2021_01_07_425794 57 1 The the DT 10_1101-2021_01_07_425794 57 2 recent recent JJ 10_1101-2021_01_07_425794 57 3 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 57 4 gene gene NN 10_1101-2021_01_07_425794 57 5 annotation annotation NN 10_1101-2021_01_07_425794 57 6 used use VBN 10_1101-2021_01_07_425794 57 7 was be VBD 10_1101-2021_01_07_425794 57 8 released release VBN 10_1101-2021_01_07_425794 57 9 by by IN 10_1101-2021_01_07_425794 57 10 the the DT 10_1101-2021_01_07_425794 57 11 NCBI NCBI NNP 10_1101-2021_01_07_425794 57 12 in in IN 10_1101-2021_01_07_425794 57 13 August August NNP 10_1101-2021_01_07_425794 57 14 2020 2020 CD 10_1101-2021_01_07_425794 57 15 . . . 10_1101-2021_01_07_425794 58 1 Its -PRON- PRP$ 10_1101-2021_01_07_425794 58 2 release release NN 10_1101-2021_01_07_425794 58 3 number number NN 10_1101-2021_01_07_425794 58 4 is be VBZ 10_1101-2021_01_07_425794 58 5 109.20200815 109.20200815 JJ 10_1101-2021_01_07_425794 58 6 and and CC 10_1101-2021_01_07_425794 58 7 it -PRON- PRP 10_1101-2021_01_07_425794 58 8 is be VBZ 10_1101-2021_01_07_425794 58 9 part part NN 10_1101-2021_01_07_425794 58 10 of of IN 10_1101-2021_01_07_425794 58 11 the the DT 10_1101-2021_01_07_425794 58 12 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 58 13 release release NN 10_1101-2021_01_07_425794 58 14 version version NN 10_1101-2021_01_07_425794 58 15 202 202 CD 10_1101-2021_01_07_425794 58 16 . . . 10_1101-2021_01_07_425794 59 1 It -PRON- PRP 10_1101-2021_01_07_425794 59 2 was be VBD 10_1101-2021_01_07_425794 59 3 downloaded download VBN 10_1101-2021_01_07_425794 59 4 from from IN 10_1101-2021_01_07_425794 59 5 the the DT 10_1101-2021_01_07_425794 59 6 NCBI NCBI NNP 10_1101-2021_01_07_425794 59 7 FTP FTP NNP 10_1101-2021_01_07_425794 59 8 site site NN 10_1101-2021_01_07_425794 59 9 ftp://ftp.ncbi.nlm.nih.gov/refseq/H ftp://ftp.ncbi.nlm.nih.gov/refseq/H NNP 10_1101-2021_01_07_425794 59 10 _ _ NNP 10_1101-2021_01_07_425794 59 11 sapiens sapien VBZ 10_1101-2021_01_07_425794 59 12 / / SYM 10_1101-2021_01_07_425794 59 13 annotation annotation NN 10_1101-2021_01_07_425794 59 14 / / SYM 10_1101-2021_01_07_425794 59 15 annotation_releases/109.20200815 annotation_releases/109.20200815 NNS 10_1101-2021_01_07_425794 59 16 / / SYM 10_1101-2021_01_07_425794 59 17 GCF_000001405.39_GRCh38 gcf_000001405.39_grch38 ADD 10_1101-2021_01_07_425794 59 18 . . . 10_1101-2021_01_07_425794 60 1 p13 p13 NNP 10_1101-2021_01_07_425794 60 2 / / SYM 10_1101-2021_01_07_425794 60 3 GCF_000001405.39_GRCh38.p13_genomic.gtf.gz gcf_000001405.39_grch38.p13_genomic.gtf.gz ADD 10_1101-2021_01_07_425794 60 4 . . . 10_1101-2021_01_07_425794 61 1 We -PRON- PRP 10_1101-2021_01_07_425794 61 2 refer refer VBP 10_1101-2021_01_07_425794 61 3 this this DT 10_1101-2021_01_07_425794 61 4 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 61 5 annota- annota- JJ 10_1101-2021_01_07_425794 61 6 tion tion NN 10_1101-2021_01_07_425794 61 7 as as IN 10_1101-2021_01_07_425794 61 8 ‘ ' `` 10_1101-2021_01_07_425794 61 9 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 61 10 - - HYPH 10_1101-2021_01_07_425794 61 11 NCBI NCBI NNP 10_1101-2021_01_07_425794 61 12 ’ ' '' 10_1101-2021_01_07_425794 61 13 in in IN 10_1101-2021_01_07_425794 61 14 this this DT 10_1101-2021_01_07_425794 61 15 study study NN 10_1101-2021_01_07_425794 61 16 . . . 10_1101-2021_01_07_425794 62 1 The the DT 10_1101-2021_01_07_425794 62 2 old old JJ 10_1101-2021_01_07_425794 62 3 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 62 4 annotation annotation NN 10_1101-2021_01_07_425794 62 5 included include VBN 10_1101-2021_01_07_425794 62 6 in in IN 10_1101-2021_01_07_425794 62 7 this this DT 10_1101-2021_01_07_425794 62 8 study study NN 10_1101-2021_01_07_425794 62 9 was be VBD 10_1101-2021_01_07_425794 62 10 released release VBN 10_1101-2021_01_07_425794 62 11 by by IN 10_1101-2021_01_07_425794 62 12 the the DT 10_1101-2021_01_07_425794 62 13 NCBI NCBI NNP 10_1101-2021_01_07_425794 62 14 in in IN 10_1101-2021_01_07_425794 62 15 April April NNP 10_1101-2021_01_07_425794 62 16 2015 2015 CD 10_1101-2021_01_07_425794 62 17 . . . 10_1101-2021_01_07_425794 63 1 It -PRON- PRP 10_1101-2021_01_07_425794 63 2 was be VBD 10_1101-2021_01_07_425794 63 3 released release VBN 10_1101-2021_01_07_425794 63 4 as as IN 10_1101-2021_01_07_425794 63 5 part part NN 10_1101-2021_01_07_425794 63 6 of of IN 10_1101-2021_01_07_425794 63 7 the the DT 10_1101-2021_01_07_425794 63 8 Patch Patch NNP 10_1101-2021_01_07_425794 63 9 2 2 CD 10_1101-2021_01_07_425794 63 10 release release NN 10_1101-2021_01_07_425794 63 11 of of IN 10_1101-2021_01_07_425794 63 12 the the DT 10_1101-2021_01_07_425794 63 13 GRCh38 GRCh38 NNP 10_1101-2021_01_07_425794 63 14 / / SYM 10_1101-2021_01_07_425794 63 15 hg38 hg38 NNP 10_1101-2021_01_07_425794 63 16 genome genome NNP 10_1101-2021_01_07_425794 63 17 build build NN 10_1101-2021_01_07_425794 63 18 . . . 10_1101-2021_01_07_425794 64 1 This this DT 10_1101-2021_01_07_425794 64 2 annotation annotation NN 10_1101-2021_01_07_425794 64 3 has have VBZ 10_1101-2021_01_07_425794 64 4 also also RB 10_1101-2021_01_07_425794 64 5 been be VBN 10_1101-2021_01_07_425794 64 6 included include VBN 10_1101-2021_01_07_425794 64 7 in in IN 10_1101-2021_01_07_425794 64 8 the the DT 10_1101-2021_01_07_425794 64 9 popular popular JJ 10_1101-2021_01_07_425794 64 10 RNA RNA NNP 10_1101-2021_01_07_425794 64 11 - - HYPH 10_1101-2021_01_07_425794 64 12 seq seq NN 10_1101-2021_01_07_425794 64 13 quantification quantification NN 10_1101-2021_01_07_425794 64 14 toolkit toolkit NNS 10_1101-2021_01_07_425794 64 15 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 64 16 [ [ -LRB- 10_1101-2021_01_07_425794 64 17 5 5 CD 10_1101-2021_01_07_425794 64 18 ] ] -RRB- 10_1101-2021_01_07_425794 64 19 as as IN 10_1101-2021_01_07_425794 64 20 the the DT 10_1101-2021_01_07_425794 64 21 default default NN 10_1101-2021_01_07_425794 64 22 annotation annotation NN 10_1101-2021_01_07_425794 64 23 used use VBN 10_1101-2021_01_07_425794 64 24 for for IN 10_1101-2021_01_07_425794 64 25 quantifying quantify VBG 10_1101-2021_01_07_425794 64 26 human human JJ 10_1101-2021_01_07_425794 64 27 RNA RNA NNP 10_1101-2021_01_07_425794 64 28 - - HYPH 10_1101-2021_01_07_425794 64 29 seq seq NN 10_1101-2021_01_07_425794 64 30 data datum NNS 10_1101-2021_01_07_425794 64 31 . . . 10_1101-2021_01_07_425794 65 1 The the DT 10_1101-2021_01_07_425794 65 2 inclusion inclusion NN 10_1101-2021_01_07_425794 65 3 of of IN 10_1101-2021_01_07_425794 65 4 this this DT 10_1101-2021_01_07_425794 65 5 old old JJ 10_1101-2021_01_07_425794 65 6 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 65 7 annotation annotation NN 10_1101-2021_01_07_425794 65 8 allowed allow VBD 10_1101-2021_01_07_425794 65 9 us -PRON- PRP 10_1101-2021_01_07_425794 65 10 to to TO 10_1101-2021_01_07_425794 65 11 investigate investigate VB 10_1101-2021_01_07_425794 65 12 how how WRB 10_1101-2021_01_07_425794 65 13 the the DT 10_1101-2021_01_07_425794 65 14 annotation annotation NN 10_1101-2021_01_07_425794 65 15 changes change NNS 10_1101-2021_01_07_425794 65 16 made make VBD 10_1101-2021_01_07_425794 65 17 recently recently RB 10_1101-2021_01_07_425794 65 18 to to IN 10_1101-2021_01_07_425794 65 19 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 65 20 affect affect VB 10_1101-2021_01_07_425794 65 21 the the DT 10_1101-2021_01_07_425794 65 22 quantification quantification NN 10_1101-2021_01_07_425794 65 23 result result NN 10_1101-2021_01_07_425794 65 24 of of IN 10_1101-2021_01_07_425794 65 25 RNA RNA NNP 10_1101-2021_01_07_425794 65 26 - - HYPH 10_1101-2021_01_07_425794 65 27 seq seq NN 10_1101-2021_01_07_425794 65 28 data datum NNS 10_1101-2021_01_07_425794 65 29 . . . 10_1101-2021_01_07_425794 66 1 The the DT 10_1101-2021_01_07_425794 66 2 4 4 CD 10_1101-2021_01_07_425794 66 3 .CC .cc SYM 10_1101-2021_01_07_425794 66 4 - - HYPH 10_1101-2021_01_07_425794 66 5 BY by IN 10_1101-2021_01_07_425794 66 6 - - HYPH 10_1101-2021_01_07_425794 66 7 NC NC NNP 10_1101-2021_01_07_425794 66 8 - - HYPH 10_1101-2021_01_07_425794 66 9 ND ND NNP 10_1101-2021_01_07_425794 66 10 4.0 4.0 CD 10_1101-2021_01_07_425794 66 11 International international JJ 10_1101-2021_01_07_425794 66 12 licensemade licensemade NN 10_1101-2021_01_07_425794 66 13 available available JJ 10_1101-2021_01_07_425794 66 14 under under IN 10_1101-2021_01_07_425794 66 15 a a DT 10_1101-2021_01_07_425794 66 16 ( ( -LRB- 10_1101-2021_01_07_425794 66 17 which which WDT 10_1101-2021_01_07_425794 66 18 was be VBD 10_1101-2021_01_07_425794 66 19 not not RB 10_1101-2021_01_07_425794 66 20 certified certify VBN 10_1101-2021_01_07_425794 66 21 by by IN 10_1101-2021_01_07_425794 66 22 peer peer NN 10_1101-2021_01_07_425794 66 23 review review NN 10_1101-2021_01_07_425794 66 24 ) ) -RRB- 10_1101-2021_01_07_425794 66 25 is be VBZ 10_1101-2021_01_07_425794 66 26 the the DT 10_1101-2021_01_07_425794 66 27 author author NN 10_1101-2021_01_07_425794 66 28 / / SYM 10_1101-2021_01_07_425794 66 29 funder funder NN 10_1101-2021_01_07_425794 66 30 , , , 10_1101-2021_01_07_425794 66 31 who who WP 10_1101-2021_01_07_425794 66 32 has have VBZ 10_1101-2021_01_07_425794 66 33 granted grant VBN 10_1101-2021_01_07_425794 66 34 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 66 35 a a DT 10_1101-2021_01_07_425794 66 36 license license NN 10_1101-2021_01_07_425794 66 37 to to TO 10_1101-2021_01_07_425794 66 38 display display VB 10_1101-2021_01_07_425794 66 39 the the DT 10_1101-2021_01_07_425794 66 40 preprint preprint NN 10_1101-2021_01_07_425794 66 41 in in IN 10_1101-2021_01_07_425794 66 42 perpetuity perpetuity NN 10_1101-2021_01_07_425794 66 43 . . . 10_1101-2021_01_07_425794 67 1 It -PRON- PRP 10_1101-2021_01_07_425794 67 2 is be VBZ 10_1101-2021_01_07_425794 67 3 The the DT 10_1101-2021_01_07_425794 67 4 copyright copyright NN 10_1101-2021_01_07_425794 67 5 holder holder NN 10_1101-2021_01_07_425794 67 6 for for IN 10_1101-2021_01_07_425794 67 7 this this DT 10_1101-2021_01_07_425794 67 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 67 9 version version NN 10_1101-2021_01_07_425794 67 10 posted post VBD 10_1101-2021_01_07_425794 67 11 January January NNP 10_1101-2021_01_07_425794 67 12 8 8 CD 10_1101-2021_01_07_425794 67 13 , , , 10_1101-2021_01_07_425794 67 14 2021 2021 CD 10_1101-2021_01_07_425794 67 15 . . . 10_1101-2021_01_07_425794 67 16 ; ; : 10_1101-2021_01_07_425794 67 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 67 18 : : : 10_1101-2021_01_07_425794 67 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 67 20 preprint preprint JJ 10_1101-2021_01_07_425794 67 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 67 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 67 23 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 67 24 annotation annotation NN 10_1101-2021_01_07_425794 67 25 in in IN 10_1101-2021_01_07_425794 67 26 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 67 27 is be VBZ 10_1101-2021_01_07_425794 67 28 slightly slightly RB 10_1101-2021_01_07_425794 67 29 different different JJ 10_1101-2021_01_07_425794 67 30 from from IN 10_1101-2021_01_07_425794 67 31 the the DT 10_1101-2021_01_07_425794 67 32 original original JJ 10_1101-2021_01_07_425794 67 33 one one NN 10_1101-2021_01_07_425794 67 34 in in IN 10_1101-2021_01_07_425794 67 35 that that IN 10_1101-2021_01_07_425794 67 36 the the DT 10_1101-2021_01_07_425794 67 37 overlapping overlapping NN 10_1101-2021_01_07_425794 67 38 exons exon NNS 10_1101-2021_01_07_425794 67 39 from from IN 10_1101-2021_01_07_425794 67 40 the the DT 10_1101-2021_01_07_425794 67 41 same same JJ 10_1101-2021_01_07_425794 67 42 gene gene NN 10_1101-2021_01_07_425794 67 43 were be VBD 10_1101-2021_01_07_425794 67 44 collapsed collapse VBN 10_1101-2021_01_07_425794 67 45 to to TO 10_1101-2021_01_07_425794 67 46 form form VB 10_1101-2021_01_07_425794 67 47 a a DT 10_1101-2021_01_07_425794 67 48 single single JJ 10_1101-2021_01_07_425794 67 49 continuous continuous JJ 10_1101-2021_01_07_425794 67 50 exon exon NN 10_1101-2021_01_07_425794 67 51 for for IN 10_1101-2021_01_07_425794 67 52 the the DT 10_1101-2021_01_07_425794 67 53 gene gene NN 10_1101-2021_01_07_425794 67 54 in in IN 10_1101-2021_01_07_425794 67 55 the the DT 10_1101-2021_01_07_425794 67 56 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 67 57 annotation annotation NN 10_1101-2021_01_07_425794 67 58 , , , 10_1101-2021_01_07_425794 67 59 however however RB 10_1101-2021_01_07_425794 67 60 this this DT 10_1101-2021_01_07_425794 67 61 difference difference NN 10_1101-2021_01_07_425794 67 62 will will MD 10_1101-2021_01_07_425794 67 63 not not RB 10_1101-2021_01_07_425794 67 64 change change VB 10_1101-2021_01_07_425794 67 65 the the DT 10_1101-2021_01_07_425794 67 66 gene gene NN 10_1101-2021_01_07_425794 67 67 - - HYPH 10_1101-2021_01_07_425794 67 68 level level NN 10_1101-2021_01_07_425794 67 69 RNA RNA NNP 10_1101-2021_01_07_425794 67 70 - - HYPH 10_1101-2021_01_07_425794 67 71 seq seq NN 10_1101-2021_01_07_425794 67 72 quantification quantification NN 10_1101-2021_01_07_425794 67 73 result result VB 10_1101-2021_01_07_425794 67 74 because because IN 10_1101-2021_01_07_425794 67 75 the the DT 10_1101-2021_01_07_425794 67 76 set set NN 10_1101-2021_01_07_425794 67 77 of of IN 10_1101-2021_01_07_425794 67 78 exonic exonic JJ 10_1101-2021_01_07_425794 67 79 bases basis NNS 10_1101-2021_01_07_425794 67 80 belonging belong VBG 10_1101-2021_01_07_425794 67 81 to to IN 10_1101-2021_01_07_425794 67 82 each each DT 10_1101-2021_01_07_425794 67 83 gene gene NN 10_1101-2021_01_07_425794 67 84 is be VBZ 10_1101-2021_01_07_425794 67 85 the the DT 10_1101-2021_01_07_425794 67 86 same same JJ 10_1101-2021_01_07_425794 67 87 between between IN 10_1101-2021_01_07_425794 67 88 the the DT 10_1101-2021_01_07_425794 67 89 original original JJ 10_1101-2021_01_07_425794 67 90 annotation annotation NN 10_1101-2021_01_07_425794 67 91 and and CC 10_1101-2021_01_07_425794 67 92 the the DT 10_1101-2021_01_07_425794 67 93 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 67 94 annotation annotation NN 10_1101-2021_01_07_425794 67 95 . . . 10_1101-2021_01_07_425794 68 1 As as IN 10_1101-2021_01_07_425794 68 2 this this DT 10_1101-2021_01_07_425794 68 3 old old JJ 10_1101-2021_01_07_425794 68 4 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 68 5 annotation annotation NN 10_1101-2021_01_07_425794 68 6 is be VBZ 10_1101-2021_01_07_425794 68 7 no no RB 10_1101-2021_01_07_425794 68 8 longer long RBR 10_1101-2021_01_07_425794 68 9 available available JJ 10_1101-2021_01_07_425794 68 10 for for IN 10_1101-2021_01_07_425794 68 11 downloading download VBG 10_1101-2021_01_07_425794 68 12 at at IN 10_1101-2021_01_07_425794 68 13 the the DT 10_1101-2021_01_07_425794 68 14 NCBI NCBI NNP 10_1101-2021_01_07_425794 68 15 FTP FTP NNP 10_1101-2021_01_07_425794 68 16 site site NN 10_1101-2021_01_07_425794 68 17 , , , 10_1101-2021_01_07_425794 68 18 we -PRON- PRP 10_1101-2021_01_07_425794 68 19 instead instead RB 10_1101-2021_01_07_425794 68 20 used use VBD 10_1101-2021_01_07_425794 68 21 the the DT 10_1101-2021_01_07_425794 68 22 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 68 23 annotation annotation NN 10_1101-2021_01_07_425794 68 24 in in IN 10_1101-2021_01_07_425794 68 25 this this DT 10_1101-2021_01_07_425794 68 26 study study NN 10_1101-2021_01_07_425794 68 27 and and CC 10_1101-2021_01_07_425794 68 28 we -PRON- PRP 10_1101-2021_01_07_425794 68 29 denote denote VBP 10_1101-2021_01_07_425794 68 30 this this DT 10_1101-2021_01_07_425794 68 31 annotation annotation NN 10_1101-2021_01_07_425794 68 32 as as IN 10_1101-2021_01_07_425794 68 33 ‘ ' `` 10_1101-2021_01_07_425794 68 34 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 68 35 - - HYPH 10_1101-2021_01_07_425794 68 36 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 68 37 ’ ' '' 10_1101-2021_01_07_425794 68 38 . . . 10_1101-2021_01_07_425794 69 1 When when WRB 10_1101-2021_01_07_425794 69 2 matching match VBG 10_1101-2021_01_07_425794 69 3 genes gene NNS 10_1101-2021_01_07_425794 69 4 from from IN 10_1101-2021_01_07_425794 69 5 different different JJ 10_1101-2021_01_07_425794 69 6 annotations annotation NNS 10_1101-2021_01_07_425794 69 7 , , , 10_1101-2021_01_07_425794 69 8 we -PRON- PRP 10_1101-2021_01_07_425794 69 9 converted convert VBD 10_1101-2021_01_07_425794 69 10 the the DT 10_1101-2021_01_07_425794 69 11 gene gene NN 10_1101-2021_01_07_425794 69 12 identifiers identifier NNS 10_1101-2021_01_07_425794 69 13 using use VBG 10_1101-2021_01_07_425794 69 14 the the DT 10_1101-2021_01_07_425794 69 15 Bioconductor Bioconductor NNP 10_1101-2021_01_07_425794 69 16 package package NN 10_1101-2021_01_07_425794 69 17 ‘ ' `` 10_1101-2021_01_07_425794 69 18 org.Hs.eg.db org.Hs.eg.db NNS 10_1101-2021_01_07_425794 69 19 ’ ' '' 10_1101-2021_01_07_425794 69 20 [ [ -LRB- 10_1101-2021_01_07_425794 69 21 17 17 CD 10_1101-2021_01_07_425794 69 22 ] ] -RRB- 10_1101-2021_01_07_425794 69 23 and and CC 10_1101-2021_01_07_425794 69 24 then then RB 10_1101-2021_01_07_425794 69 25 compared compare VBD 10_1101-2021_01_07_425794 69 26 them -PRON- PRP 10_1101-2021_01_07_425794 69 27 to to TO 10_1101-2021_01_07_425794 69 28 find find VB 10_1101-2021_01_07_425794 69 29 common common JJ 10_1101-2021_01_07_425794 69 30 genes gene NNS 10_1101-2021_01_07_425794 69 31 between between IN 10_1101-2021_01_07_425794 69 32 annotations annotation NNS 10_1101-2021_01_07_425794 69 33 . . . 10_1101-2021_01_07_425794 70 1 2.3 2.3 CD 10_1101-2021_01_07_425794 70 2 Mapping mapping NN 10_1101-2021_01_07_425794 70 3 , , , 10_1101-2021_01_07_425794 70 4 quantification quantification NN 10_1101-2021_01_07_425794 70 5 and and CC 10_1101-2021_01_07_425794 70 6 normalization normalization NN 10_1101-2021_01_07_425794 70 7 of of IN 10_1101-2021_01_07_425794 70 8 RNA RNA NNP 10_1101-2021_01_07_425794 70 9 - - HYPH 10_1101-2021_01_07_425794 70 10 seq seq NN 10_1101-2021_01_07_425794 70 11 data datum NNS 10_1101-2021_01_07_425794 70 12 Analysis analysis NN 10_1101-2021_01_07_425794 70 13 of of IN 10_1101-2021_01_07_425794 70 14 the the DT 10_1101-2021_01_07_425794 70 15 RNA RNA NNP 10_1101-2021_01_07_425794 70 16 - - HYPH 10_1101-2021_01_07_425794 70 17 seq seq NN 10_1101-2021_01_07_425794 70 18 data datum NNS 10_1101-2021_01_07_425794 70 19 was be VBD 10_1101-2021_01_07_425794 70 20 performed perform VBN 10_1101-2021_01_07_425794 70 21 using use VBG 10_1101-2021_01_07_425794 70 22 Bioconductor Bioconductor NNP 10_1101-2021_01_07_425794 70 23 R R NNP 10_1101-2021_01_07_425794 70 24 packages package NNS 10_1101-2021_01_07_425794 70 25 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 70 26 and and CC 10_1101-2021_01_07_425794 70 27 limma limma VB 10_1101-2021_01_07_425794 70 28 [ [ -LRB- 10_1101-2021_01_07_425794 70 29 5 5 CD 10_1101-2021_01_07_425794 70 30 , , , 10_1101-2021_01_07_425794 70 31 18 18 CD 10_1101-2021_01_07_425794 70 32 , , , 10_1101-2021_01_07_425794 70 33 19 19 CD 10_1101-2021_01_07_425794 70 34 ] ] -RRB- 10_1101-2021_01_07_425794 70 35 . . . 10_1101-2021_01_07_425794 71 1 The the DT 10_1101-2021_01_07_425794 71 2 human human JJ 10_1101-2021_01_07_425794 71 3 reference reference NN 10_1101-2021_01_07_425794 71 4 genome genome NN 10_1101-2021_01_07_425794 71 5 ( ( -LRB- 10_1101-2021_01_07_425794 71 6 GRCh38 grch38 NN 10_1101-2021_01_07_425794 71 7 ) ) -RRB- 10_1101-2021_01_07_425794 71 8 from from IN 10_1101-2021_01_07_425794 71 9 GENCODE GENCODE NNP 10_1101-2021_01_07_425794 71 10 ( ( -LRB- 10_1101-2021_01_07_425794 71 11 version version NN 10_1101-2021_01_07_425794 71 12 34 34 CD 10_1101-2021_01_07_425794 71 13 downloaded download VBN 10_1101-2021_01_07_425794 71 14 from from IN 10_1101-2021_01_07_425794 71 15 ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/ ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/ NNP 10_1101-2021_01_07_425794 71 16 release_34 release_34 NNP 10_1101-2021_01_07_425794 71 17 / / SYM 10_1101-2021_01_07_425794 71 18 GRCh38.primary_assembly.genome.fa.gz GRCh38.primary_assembly.genome.fa.gz NNS 10_1101-2021_01_07_425794 71 19 ) ) -RRB- 10_1101-2021_01_07_425794 71 20 was be VBD 10_1101-2021_01_07_425794 71 21 indexed index VBN 10_1101-2021_01_07_425794 71 22 using use VBG 10_1101-2021_01_07_425794 71 23 the the DT 10_1101-2021_01_07_425794 71 24 buildin- buildin- NN 10_1101-2021_01_07_425794 71 25 dex dex NNP 10_1101-2021_01_07_425794 71 26 function function NN 10_1101-2021_01_07_425794 71 27 in in IN 10_1101-2021_01_07_425794 71 28 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 71 29 v2.2.6 v2.2.6 NNP 10_1101-2021_01_07_425794 71 30 [ [ -LRB- 10_1101-2021_01_07_425794 71 31 5 5 CD 10_1101-2021_01_07_425794 71 32 ] ] -RRB- 10_1101-2021_01_07_425794 71 33 . . . 10_1101-2021_01_07_425794 72 1 Sequencing sequencing NN 10_1101-2021_01_07_425794 72 2 reads read NNS 10_1101-2021_01_07_425794 72 3 were be VBD 10_1101-2021_01_07_425794 72 4 then then RB 10_1101-2021_01_07_425794 72 5 mapped map VBN 10_1101-2021_01_07_425794 72 6 to to IN 10_1101-2021_01_07_425794 72 7 the the DT 10_1101-2021_01_07_425794 72 8 reference reference NN 10_1101-2021_01_07_425794 72 9 genome genome NN 10_1101-2021_01_07_425794 72 10 using use VBG 10_1101-2021_01_07_425794 72 11 the the DT 10_1101-2021_01_07_425794 72 12 align align NN 10_1101-2021_01_07_425794 72 13 function function NN 10_1101-2021_01_07_425794 72 14 in in IN 10_1101-2021_01_07_425794 72 15 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 72 16 [ [ -LRB- 10_1101-2021_01_07_425794 72 17 5 5 CD 10_1101-2021_01_07_425794 72 18 , , , 10_1101-2021_01_07_425794 72 19 20 20 CD 10_1101-2021_01_07_425794 72 20 ] ] -RRB- 10_1101-2021_01_07_425794 72 21 . . . 10_1101-2021_01_07_425794 73 1 During during IN 10_1101-2021_01_07_425794 73 2 the the DT 10_1101-2021_01_07_425794 73 3 alignment alignment NN 10_1101-2021_01_07_425794 73 4 , , , 10_1101-2021_01_07_425794 73 5 the the DT 10_1101-2021_01_07_425794 73 6 En- en- CD 10_1101-2021_01_07_425794 73 7 sembl sembl NN 10_1101-2021_01_07_425794 73 8 , , , 10_1101-2021_01_07_425794 73 9 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 73 10 - - HYPH 10_1101-2021_01_07_425794 73 11 NCBI NCBI NNP 10_1101-2021_01_07_425794 73 12 and and CC 10_1101-2021_01_07_425794 73 13 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 73 14 - - HYPH 10_1101-2021_01_07_425794 73 15 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 73 16 annotations annotation NNS 10_1101-2021_01_07_425794 73 17 were be VBD 10_1101-2021_01_07_425794 73 18 also also RB 10_1101-2021_01_07_425794 73 19 included include VBN 10_1101-2021_01_07_425794 73 20 as as IN 10_1101-2021_01_07_425794 73 21 an an DT 10_1101-2021_01_07_425794 73 22 extra extra JJ 10_1101-2021_01_07_425794 73 23 parameter parameter NN 10_1101-2021_01_07_425794 73 24 to to TO 10_1101-2021_01_07_425794 73 25 improve improve VB 10_1101-2021_01_07_425794 73 26 alignment alignment NN 10_1101-2021_01_07_425794 73 27 . . . 10_1101-2021_01_07_425794 74 1 Gene gene NN 10_1101-2021_01_07_425794 74 2 - - HYPH 10_1101-2021_01_07_425794 74 3 level level NN 10_1101-2021_01_07_425794 74 4 read read NN 10_1101-2021_01_07_425794 74 5 counts count NNS 10_1101-2021_01_07_425794 74 6 were be VBD 10_1101-2021_01_07_425794 74 7 obtained obtain VBN 10_1101-2021_01_07_425794 74 8 with with IN 10_1101-2021_01_07_425794 74 9 featureCounts featurecount NNS 10_1101-2021_01_07_425794 74 10 [ [ -LRB- 10_1101-2021_01_07_425794 74 11 4 4 CD 10_1101-2021_01_07_425794 74 12 , , , 10_1101-2021_01_07_425794 74 13 5 5 CD 10_1101-2021_01_07_425794 74 14 ] ] -RRB- 10_1101-2021_01_07_425794 74 15 , , , 10_1101-2021_01_07_425794 74 16 a a DT 10_1101-2021_01_07_425794 74 17 read read NN 10_1101-2021_01_07_425794 74 18 count count NN 10_1101-2021_01_07_425794 74 19 summa- summa- JJ 10_1101-2021_01_07_425794 74 20 rization rization NN 10_1101-2021_01_07_425794 74 21 function function NN 10_1101-2021_01_07_425794 74 22 within within IN 10_1101-2021_01_07_425794 74 23 the the DT 10_1101-2021_01_07_425794 74 24 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 74 25 package package NN 10_1101-2021_01_07_425794 74 26 . . . 10_1101-2021_01_07_425794 75 1 The the DT 10_1101-2021_01_07_425794 75 2 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 75 3 , , , 10_1101-2021_01_07_425794 75 4 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 75 5 - - HYPH 10_1101-2021_01_07_425794 75 6 NCBI NCBI NNP 10_1101-2021_01_07_425794 75 7 and and CC 10_1101-2021_01_07_425794 75 8 RefSeq- RefSeq- NNP 10_1101-2021_01_07_425794 75 9 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 75 10 annotations annotation NNS 10_1101-2021_01_07_425794 75 11 were be VBD 10_1101-2021_01_07_425794 75 12 provided provide VBN 10_1101-2021_01_07_425794 75 13 to to IN 10_1101-2021_01_07_425794 75 14 featureCounts featurecount NNS 10_1101-2021_01_07_425794 75 15 to to TO 10_1101-2021_01_07_425794 75 16 generate generate VB 10_1101-2021_01_07_425794 75 17 read read VBN 10_1101-2021_01_07_425794 75 18 counts count NNS 10_1101-2021_01_07_425794 75 19 for for IN 10_1101-2021_01_07_425794 75 20 genes gene NNS 10_1101-2021_01_07_425794 75 21 included include VBN 10_1101-2021_01_07_425794 75 22 in in IN 10_1101-2021_01_07_425794 75 23 these these DT 10_1101-2021_01_07_425794 75 24 annotations annotation NNS 10_1101-2021_01_07_425794 75 25 respectively respectively RB 10_1101-2021_01_07_425794 75 26 . . . 10_1101-2021_01_07_425794 76 1 The the DT 10_1101-2021_01_07_425794 76 2 gene gene NN 10_1101-2021_01_07_425794 76 3 - - HYPH 10_1101-2021_01_07_425794 76 4 level level NN 10_1101-2021_01_07_425794 76 5 read read NN 10_1101-2021_01_07_425794 76 6 counts count NNS 10_1101-2021_01_07_425794 76 7 were be VBD 10_1101-2021_01_07_425794 76 8 transformed transform VBN 10_1101-2021_01_07_425794 76 9 using use VBG 10_1101-2021_01_07_425794 76 10 the the DT 10_1101-2021_01_07_425794 76 11 voom voom JJ 10_1101-2021_01_07_425794 76 12 function function NN 10_1101-2021_01_07_425794 76 13 in in IN 10_1101-2021_01_07_425794 76 14 limma limma NNP 10_1101-2021_01_07_425794 76 15 [ [ -LRB- 10_1101-2021_01_07_425794 76 16 18 18 CD 10_1101-2021_01_07_425794 76 17 , , , 10_1101-2021_01_07_425794 76 18 21 21 CD 10_1101-2021_01_07_425794 76 19 ] ] -RRB- 10_1101-2021_01_07_425794 76 20 and and CC 10_1101-2021_01_07_425794 76 21 then then RB 10_1101-2021_01_07_425794 76 22 normalized normalize VBD 10_1101-2021_01_07_425794 76 23 using use VBG 10_1101-2021_01_07_425794 76 24 the the DT 10_1101-2021_01_07_425794 76 25 library library NN 10_1101-2021_01_07_425794 76 26 size size NN 10_1101-2021_01_07_425794 76 27 [ [ -LRB- 10_1101-2021_01_07_425794 76 28 22 22 CD 10_1101-2021_01_07_425794 76 29 ] ] -RRB- 10_1101-2021_01_07_425794 76 30 , , , 10_1101-2021_01_07_425794 76 31 quantile quantile NNP 10_1101-2021_01_07_425794 76 32 [ [ -LRB- 10_1101-2021_01_07_425794 76 33 23 23 CD 10_1101-2021_01_07_425794 76 34 ] ] -RRB- 10_1101-2021_01_07_425794 76 35 and and CC 10_1101-2021_01_07_425794 76 36 trimmed trim VBN 10_1101-2021_01_07_425794 76 37 mean mean NN 10_1101-2021_01_07_425794 76 38 of of IN 10_1101-2021_01_07_425794 76 39 M- M- NNP 10_1101-2021_01_07_425794 76 40 values value NNS 10_1101-2021_01_07_425794 76 41 ( ( -LRB- 10_1101-2021_01_07_425794 76 42 TMM TMM NNP 10_1101-2021_01_07_425794 76 43 ) ) -RRB- 10_1101-2021_01_07_425794 76 44 [ [ -LRB- 10_1101-2021_01_07_425794 76 45 24 24 CD 10_1101-2021_01_07_425794 76 46 ] ] -RRB- 10_1101-2021_01_07_425794 76 47 methods method NNS 10_1101-2021_01_07_425794 76 48 , , , 10_1101-2021_01_07_425794 76 49 respectively respectively RB 10_1101-2021_01_07_425794 76 50 , , , 10_1101-2021_01_07_425794 76 51 prior prior RB 10_1101-2021_01_07_425794 76 52 to to IN 10_1101-2021_01_07_425794 76 53 performing perform VBG 10_1101-2021_01_07_425794 76 54 further further JJ 10_1101-2021_01_07_425794 76 55 analysis analysis NN 10_1101-2021_01_07_425794 76 56 . . . 10_1101-2021_01_07_425794 77 1 The the DT 10_1101-2021_01_07_425794 77 2 library library NN 10_1101-2021_01_07_425794 77 3 size size NN 10_1101-2021_01_07_425794 77 4 normalization normalization NN 10_1101-2021_01_07_425794 77 5 was be VBD 10_1101-2021_01_07_425794 77 6 performed perform VBN 10_1101-2021_01_07_425794 77 7 by by IN 10_1101-2021_01_07_425794 77 8 providing provide VBG 10_1101-2021_01_07_425794 77 9 raw raw JJ 10_1101-2021_01_07_425794 77 10 read read VB 10_1101-2021_01_07_425794 77 11 counts count NNS 10_1101-2021_01_07_425794 77 12 to to TO 10_1101-2021_01_07_425794 77 13 voom voom VB 10_1101-2021_01_07_425794 77 14 and and CC 10_1101-2021_01_07_425794 77 15 then then RB 10_1101-2021_01_07_425794 77 16 running run VBG 10_1101-2021_01_07_425794 77 17 voom voom NN 10_1101-2021_01_07_425794 77 18 with with IN 10_1101-2021_01_07_425794 77 19 the the DT 10_1101-2021_01_07_425794 77 20 ‘ ' `` 10_1101-2021_01_07_425794 77 21 normalize.method normalize.method NNP 10_1101-2021_01_07_425794 77 22 ’ ' '' 10_1101-2021_01_07_425794 77 23 parameter parameter NN 10_1101-2021_01_07_425794 77 24 set set VBN 10_1101-2021_01_07_425794 77 25 to to IN 10_1101-2021_01_07_425794 77 26 ‘ ' `` 10_1101-2021_01_07_425794 77 27 none none NN 10_1101-2021_01_07_425794 77 28 ’ ' '' 10_1101-2021_01_07_425794 77 29 . . . 10_1101-2021_01_07_425794 78 1 The the DT 10_1101-2021_01_07_425794 78 2 quantile quantile NN 10_1101-2021_01_07_425794 78 3 nor- nor- NN 10_1101-2021_01_07_425794 78 4 malization malization NN 10_1101-2021_01_07_425794 78 5 was be VBD 10_1101-2021_01_07_425794 78 6 performed perform VBN 10_1101-2021_01_07_425794 78 7 by by IN 10_1101-2021_01_07_425794 78 8 providing provide VBG 10_1101-2021_01_07_425794 78 9 raw raw JJ 10_1101-2021_01_07_425794 78 10 read read VB 10_1101-2021_01_07_425794 78 11 counts count NNS 10_1101-2021_01_07_425794 78 12 to to TO 10_1101-2021_01_07_425794 78 13 voom voom VB 10_1101-2021_01_07_425794 78 14 and and CC 10_1101-2021_01_07_425794 78 15 then then RB 10_1101-2021_01_07_425794 78 16 running run VBG 10_1101-2021_01_07_425794 78 17 voom voom NN 10_1101-2021_01_07_425794 78 18 with with IN 10_1101-2021_01_07_425794 78 19 the the DT 10_1101-2021_01_07_425794 78 20 ‘ ' `` 10_1101-2021_01_07_425794 78 21 normalize.method normalize.method NNP 10_1101-2021_01_07_425794 78 22 ’ ' '' 10_1101-2021_01_07_425794 78 23 parameter parameter NN 10_1101-2021_01_07_425794 78 24 set set VBN 10_1101-2021_01_07_425794 78 25 to to IN 10_1101-2021_01_07_425794 78 26 ‘ ' `` 10_1101-2021_01_07_425794 78 27 quantile quantile VB 10_1101-2021_01_07_425794 78 28 ’ ' '' 10_1101-2021_01_07_425794 78 29 . . . 10_1101-2021_01_07_425794 79 1 For for IN 10_1101-2021_01_07_425794 79 2 TMM TMM NNP 10_1101-2021_01_07_425794 79 3 normalization normalization NN 10_1101-2021_01_07_425794 79 4 , , , 10_1101-2021_01_07_425794 79 5 we -PRON- PRP 10_1101-2021_01_07_425794 79 6 first first RB 10_1101-2021_01_07_425794 79 7 calculated calculate VBD 10_1101-2021_01_07_425794 79 8 the the DT 10_1101-2021_01_07_425794 79 9 TMM TMM NNP 10_1101-2021_01_07_425794 79 10 normalization normalization NN 10_1101-2021_01_07_425794 79 11 factor factor NN 10_1101-2021_01_07_425794 79 12 for for IN 10_1101-2021_01_07_425794 79 13 each each DT 10_1101-2021_01_07_425794 79 14 library library NN 10_1101-2021_01_07_425794 79 15 using use VBG 10_1101-2021_01_07_425794 79 16 the the DT 10_1101-2021_01_07_425794 79 17 calcNormFactors calcNormFactors NNP 10_1101-2021_01_07_425794 79 18 method method NN 10_1101-2021_01_07_425794 79 19 in in IN 10_1101-2021_01_07_425794 79 20 edgeR edgeR NNP 10_1101-2021_01_07_425794 79 21 [ [ -LRB- 10_1101-2021_01_07_425794 79 22 25 25 CD 10_1101-2021_01_07_425794 79 23 ] ] -RRB- 10_1101-2021_01_07_425794 79 24 . . . 10_1101-2021_01_07_425794 80 1 Then then RB 10_1101-2021_01_07_425794 80 2 we -PRON- PRP 10_1101-2021_01_07_425794 80 3 provided provide VBD 10_1101-2021_01_07_425794 80 4 raw raw JJ 10_1101-2021_01_07_425794 80 5 read read NN 10_1101-2021_01_07_425794 80 6 counts count NNS 10_1101-2021_01_07_425794 80 7 and and CC 10_1101-2021_01_07_425794 80 8 the the DT 10_1101-2021_01_07_425794 80 9 TMM TMM NNP 10_1101-2021_01_07_425794 80 10 normalization normalization NN 10_1101-2021_01_07_425794 80 11 factors factor VBZ 10_1101-2021_01_07_425794 80 12 to to TO 10_1101-2021_01_07_425794 80 13 voom voom VB 10_1101-2021_01_07_425794 80 14 and and CC 10_1101-2021_01_07_425794 80 15 ran run VBD 10_1101-2021_01_07_425794 80 16 it -PRON- PRP 10_1101-2021_01_07_425794 80 17 with with IN 10_1101-2021_01_07_425794 80 18 the the DT 10_1101-2021_01_07_425794 80 19 ‘ ' `` 10_1101-2021_01_07_425794 80 20 normalize.method normalize.method NNP 10_1101-2021_01_07_425794 80 21 ’ ' '' 10_1101-2021_01_07_425794 80 22 parameter parameter NN 10_1101-2021_01_07_425794 80 23 set set VBN 10_1101-2021_01_07_425794 80 24 to to IN 10_1101-2021_01_07_425794 80 25 ‘ ' `` 10_1101-2021_01_07_425794 80 26 none none NN 10_1101-2021_01_07_425794 80 27 ’ ' '' 10_1101-2021_01_07_425794 80 28 . . . 10_1101-2021_01_07_425794 81 1 The the DT 10_1101-2021_01_07_425794 81 2 log2CPM log2CPM NNP 10_1101-2021_01_07_425794 81 3 ( ( -LRB- 10_1101-2021_01_07_425794 81 4 log2 log2 NNP 10_1101-2021_01_07_425794 81 5 counts count NNS 10_1101-2021_01_07_425794 81 6 per per IN 10_1101-2021_01_07_425794 81 7 million million CD 10_1101-2021_01_07_425794 81 8 ) ) -RRB- 10_1101-2021_01_07_425794 81 9 values value NNS 10_1101-2021_01_07_425794 81 10 , , , 10_1101-2021_01_07_425794 81 11 produced produce VBN 10_1101-2021_01_07_425794 81 12 by by IN 10_1101-2021_01_07_425794 81 13 the the DT 10_1101-2021_01_07_425794 81 14 voom voom JJ 10_1101-2021_01_07_425794 81 15 function function NN 10_1101-2021_01_07_425794 81 16 for for IN 10_1101-2021_01_07_425794 81 17 each each DT 10_1101-2021_01_07_425794 81 18 gene gene NN 10_1101-2021_01_07_425794 81 19 5 5 CD 10_1101-2021_01_07_425794 81 20 .CC .CC , 10_1101-2021_01_07_425794 81 21 - - HYPH 10_1101-2021_01_07_425794 81 22 BY by IN 10_1101-2021_01_07_425794 81 23 - - HYPH 10_1101-2021_01_07_425794 81 24 NC NC NNP 10_1101-2021_01_07_425794 81 25 - - HYPH 10_1101-2021_01_07_425794 81 26 ND ND NNP 10_1101-2021_01_07_425794 81 27 4.0 4.0 CD 10_1101-2021_01_07_425794 81 28 International international JJ 10_1101-2021_01_07_425794 81 29 licensemade licensemade NN 10_1101-2021_01_07_425794 81 30 available available JJ 10_1101-2021_01_07_425794 81 31 under under IN 10_1101-2021_01_07_425794 81 32 a a DT 10_1101-2021_01_07_425794 81 33 ( ( -LRB- 10_1101-2021_01_07_425794 81 34 which which WDT 10_1101-2021_01_07_425794 81 35 was be VBD 10_1101-2021_01_07_425794 81 36 not not RB 10_1101-2021_01_07_425794 81 37 certified certify VBN 10_1101-2021_01_07_425794 81 38 by by IN 10_1101-2021_01_07_425794 81 39 peer peer NN 10_1101-2021_01_07_425794 81 40 review review NN 10_1101-2021_01_07_425794 81 41 ) ) -RRB- 10_1101-2021_01_07_425794 81 42 is be VBZ 10_1101-2021_01_07_425794 81 43 the the DT 10_1101-2021_01_07_425794 81 44 author author NN 10_1101-2021_01_07_425794 81 45 / / SYM 10_1101-2021_01_07_425794 81 46 funder funder NN 10_1101-2021_01_07_425794 81 47 , , , 10_1101-2021_01_07_425794 81 48 who who WP 10_1101-2021_01_07_425794 81 49 has have VBZ 10_1101-2021_01_07_425794 81 50 granted grant VBN 10_1101-2021_01_07_425794 81 51 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 81 52 a a DT 10_1101-2021_01_07_425794 81 53 license license NN 10_1101-2021_01_07_425794 81 54 to to TO 10_1101-2021_01_07_425794 81 55 display display VB 10_1101-2021_01_07_425794 81 56 the the DT 10_1101-2021_01_07_425794 81 57 preprint preprint NN 10_1101-2021_01_07_425794 81 58 in in IN 10_1101-2021_01_07_425794 81 59 perpetuity perpetuity NN 10_1101-2021_01_07_425794 81 60 . . . 10_1101-2021_01_07_425794 82 1 It -PRON- PRP 10_1101-2021_01_07_425794 82 2 is be VBZ 10_1101-2021_01_07_425794 82 3 The the DT 10_1101-2021_01_07_425794 82 4 copyright copyright NN 10_1101-2021_01_07_425794 82 5 holder holder NN 10_1101-2021_01_07_425794 82 6 for for IN 10_1101-2021_01_07_425794 82 7 this this DT 10_1101-2021_01_07_425794 82 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 82 9 version version NN 10_1101-2021_01_07_425794 82 10 posted post VBD 10_1101-2021_01_07_425794 82 11 January January NNP 10_1101-2021_01_07_425794 82 12 8 8 CD 10_1101-2021_01_07_425794 82 13 , , , 10_1101-2021_01_07_425794 82 14 2021 2021 CD 10_1101-2021_01_07_425794 82 15 . . . 10_1101-2021_01_07_425794 82 16 ; ; : 10_1101-2021_01_07_425794 82 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 82 18 : : : 10_1101-2021_01_07_425794 82 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 82 20 preprint preprint JJ 10_1101-2021_01_07_425794 82 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 82 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 82 23 in in IN 10_1101-2021_01_07_425794 82 24 each each DT 10_1101-2021_01_07_425794 82 25 library library NN 10_1101-2021_01_07_425794 82 26 , , , 10_1101-2021_01_07_425794 82 27 were be VBD 10_1101-2021_01_07_425794 82 28 converted convert VBN 10_1101-2021_01_07_425794 82 29 to to IN 10_1101-2021_01_07_425794 82 30 log2FPKM log2FPKM NNP 10_1101-2021_01_07_425794 82 31 ( ( -LRB- 10_1101-2021_01_07_425794 82 32 log2 log2 NN 10_1101-2021_01_07_425794 82 33 fragments fragment NNS 10_1101-2021_01_07_425794 82 34 per per IN 10_1101-2021_01_07_425794 82 35 kilo kilo NNP 10_1101-2021_01_07_425794 82 36 exonic exonic JJ 10_1101-2021_01_07_425794 82 37 bases basis NNS 10_1101-2021_01_07_425794 82 38 per per IN 10_1101-2021_01_07_425794 82 39 million million CD 10_1101-2021_01_07_425794 82 40 mapped map VBN 10_1101-2021_01_07_425794 82 41 fragments fragment NNS 10_1101-2021_01_07_425794 82 42 ) ) -RRB- 10_1101-2021_01_07_425794 82 43 expression expression NN 10_1101-2021_01_07_425794 82 44 values value NNS 10_1101-2021_01_07_425794 82 45 for for IN 10_1101-2021_01_07_425794 82 46 further further JJ 10_1101-2021_01_07_425794 82 47 analysis analysis NN 10_1101-2021_01_07_425794 82 48 . . . 10_1101-2021_01_07_425794 83 1 2.4 2.4 CD 10_1101-2021_01_07_425794 83 2 Titration titration NN 10_1101-2021_01_07_425794 83 3 monotonicity monotonicity NN 10_1101-2021_01_07_425794 83 4 The the DT 10_1101-2021_01_07_425794 83 5 RNA RNA NNP 10_1101-2021_01_07_425794 83 6 - - HYPH 10_1101-2021_01_07_425794 83 7 seq seq NN 10_1101-2021_01_07_425794 83 8 data datum NNS 10_1101-2021_01_07_425794 83 9 from from IN 10_1101-2021_01_07_425794 83 10 the the DT 10_1101-2021_01_07_425794 83 11 SEQC SEQC NNP 10_1101-2021_01_07_425794 83 12 project project NN 10_1101-2021_01_07_425794 83 13 have have VBP 10_1101-2021_01_07_425794 83 14 titration titration NN 10_1101-2021_01_07_425794 83 15 monotonicity monotonicity NN 10_1101-2021_01_07_425794 83 16 built build VBN 10_1101-2021_01_07_425794 83 17 into into IN 10_1101-2021_01_07_425794 83 18 them -PRON- PRP 10_1101-2021_01_07_425794 83 19 , , , 10_1101-2021_01_07_425794 83 20 such such JJ 10_1101-2021_01_07_425794 83 21 that that IN 10_1101-2021_01_07_425794 83 22 a a DT 10_1101-2021_01_07_425794 83 23 gene gene NN 10_1101-2021_01_07_425794 83 24 is be VBZ 10_1101-2021_01_07_425794 83 25 considered consider VBN 10_1101-2021_01_07_425794 83 26 to to TO 10_1101-2021_01_07_425794 83 27 preserve preserve VB 10_1101-2021_01_07_425794 83 28 titration titration NN 10_1101-2021_01_07_425794 83 29 monotonicity monotonicity NN 10_1101-2021_01_07_425794 83 30 if if IN 10_1101-2021_01_07_425794 83 31 the the DT 10_1101-2021_01_07_425794 83 32 expression expression NN 10_1101-2021_01_07_425794 83 33 of of IN 10_1101-2021_01_07_425794 83 34 the the DT 10_1101-2021_01_07_425794 83 35 gene gene NN 10_1101-2021_01_07_425794 83 36 follows follow VBZ 10_1101-2021_01_07_425794 83 37 A a DT 10_1101-2021_01_07_425794 83 38 ≥ ≥ NN 10_1101-2021_01_07_425794 83 39 C≥ c≥ NN 10_1101-2021_01_07_425794 83 40 D d NN 10_1101-2021_01_07_425794 83 41 ≥B ≥b NN 10_1101-2021_01_07_425794 83 42 when when WRB 10_1101-2021_01_07_425794 83 43 its -PRON- PRP$ 10_1101-2021_01_07_425794 83 44 expression expression NN 10_1101-2021_01_07_425794 83 45 in in IN 10_1101-2021_01_07_425794 83 46 sample sample NN 10_1101-2021_01_07_425794 83 47 A A NNP 10_1101-2021_01_07_425794 83 48 is be VBZ 10_1101-2021_01_07_425794 83 49 greater great JJR 10_1101-2021_01_07_425794 83 50 than than IN 10_1101-2021_01_07_425794 83 51 or or CC 10_1101-2021_01_07_425794 83 52 equal equal JJ 10_1101-2021_01_07_425794 83 53 to to IN 10_1101-2021_01_07_425794 83 54 that that DT 10_1101-2021_01_07_425794 83 55 in in IN 10_1101-2021_01_07_425794 83 56 sample sample NN 10_1101-2021_01_07_425794 83 57 B B NNP 10_1101-2021_01_07_425794 83 58 , , , 10_1101-2021_01_07_425794 83 59 or or CC 10_1101-2021_01_07_425794 83 60 follows follow VBZ 10_1101-2021_01_07_425794 83 61 A a DT 10_1101-2021_01_07_425794 83 62 ≤ ≤ NN 10_1101-2021_01_07_425794 83 63 C≤ c≤ NN 10_1101-2021_01_07_425794 83 64 D D NNP 10_1101-2021_01_07_425794 83 65 ≤B ≤b NN 10_1101-2021_01_07_425794 83 66 when when WRB 10_1101-2021_01_07_425794 83 67 its -PRON- PRP$ 10_1101-2021_01_07_425794 83 68 expression expression NN 10_1101-2021_01_07_425794 83 69 in in IN 10_1101-2021_01_07_425794 83 70 sample sample NN 10_1101-2021_01_07_425794 83 71 A A NNP 10_1101-2021_01_07_425794 83 72 is be VBZ 10_1101-2021_01_07_425794 83 73 less less JJR 10_1101-2021_01_07_425794 83 74 than than IN 10_1101-2021_01_07_425794 83 75 or or CC 10_1101-2021_01_07_425794 83 76 equal equal JJ 10_1101-2021_01_07_425794 83 77 to to IN 10_1101-2021_01_07_425794 83 78 that that DT 10_1101-2021_01_07_425794 83 79 in in IN 10_1101-2021_01_07_425794 83 80 sample sample NN 10_1101-2021_01_07_425794 83 81 B. B. NNP 10_1101-2021_01_07_425794 84 1 To to TO 10_1101-2021_01_07_425794 84 2 test test VB 10_1101-2021_01_07_425794 84 3 if if IN 10_1101-2021_01_07_425794 84 4 the the DT 10_1101-2021_01_07_425794 84 5 titration titration NN 10_1101-2021_01_07_425794 84 6 monotonicity monotonicity NN 10_1101-2021_01_07_425794 84 7 is be VBZ 10_1101-2021_01_07_425794 84 8 preserved preserve VBN 10_1101-2021_01_07_425794 84 9 , , , 10_1101-2021_01_07_425794 84 10 Equation Equation NNP 10_1101-2021_01_07_425794 84 11 ( ( -LRB- 10_1101-2021_01_07_425794 84 12 1 1 CD 10_1101-2021_01_07_425794 84 13 ) ) -RRB- 10_1101-2021_01_07_425794 84 14 was be VBD 10_1101-2021_01_07_425794 84 15 used use VBN 10_1101-2021_01_07_425794 84 16 to to TO 10_1101-2021_01_07_425794 84 17 compute compute VB 10_1101-2021_01_07_425794 84 18 the the DT 10_1101-2021_01_07_425794 84 19 expected expect VBN 10_1101-2021_01_07_425794 84 20 log2 log2 NN 10_1101-2021_01_07_425794 84 21 fold fold NN 10_1101-2021_01_07_425794 84 22 - - HYPH 10_1101-2021_01_07_425794 84 23 change change NN 10_1101-2021_01_07_425794 84 24 for for IN 10_1101-2021_01_07_425794 84 25 a a DT 10_1101-2021_01_07_425794 84 26 gene gene NN 10_1101-2021_01_07_425794 84 27 in in IN 10_1101-2021_01_07_425794 84 28 the the DT 10_1101-2021_01_07_425794 84 29 comparison comparison NN 10_1101-2021_01_07_425794 84 30 of of IN 10_1101-2021_01_07_425794 84 31 C C NNP 10_1101-2021_01_07_425794 84 32 vs vs IN 10_1101-2021_01_07_425794 84 33 D D NNP 10_1101-2021_01_07_425794 84 34 given give VBN 10_1101-2021_01_07_425794 84 35 the the DT 10_1101-2021_01_07_425794 84 36 log2 log2 NN 10_1101-2021_01_07_425794 84 37 fold fold NN 10_1101-2021_01_07_425794 84 38 - - HYPH 10_1101-2021_01_07_425794 84 39 change change NN 10_1101-2021_01_07_425794 84 40 between between IN 10_1101-2021_01_07_425794 84 41 A A NNP 10_1101-2021_01_07_425794 84 42 vs vs FW 10_1101-2021_01_07_425794 84 43 B. B. NNP 10_1101-2021_01_07_425794 84 44 E e NN 10_1101-2021_01_07_425794 84 45 = = SYM 10_1101-2021_01_07_425794 84 46 log2 log2 NN 10_1101-2021_01_07_425794 84 47 ( ( -LRB- 10_1101-2021_01_07_425794 84 48 3 3 CD 10_1101-2021_01_07_425794 84 49 × × CD 10_1101-2021_01_07_425794 84 50 2x 2x CD 10_1101-2021_01_07_425794 84 51 + + SYM 10_1101-2021_01_07_425794 84 52 1 1 CD 10_1101-2021_01_07_425794 84 53 2x 2x CD 10_1101-2021_01_07_425794 84 54 + + SYM 10_1101-2021_01_07_425794 84 55 3 3 CD 10_1101-2021_01_07_425794 84 56 ) ) -RRB- 10_1101-2021_01_07_425794 84 57 ( ( -LRB- 10_1101-2021_01_07_425794 84 58 1 1 LS 10_1101-2021_01_07_425794 84 59 ) ) -RRB- 10_1101-2021_01_07_425794 84 60 where where WRB 10_1101-2021_01_07_425794 84 61 E e NN 10_1101-2021_01_07_425794 84 62 is be VBZ 10_1101-2021_01_07_425794 84 63 the the DT 10_1101-2021_01_07_425794 84 64 expected expected JJ 10_1101-2021_01_07_425794 84 65 log2 log2 NN 10_1101-2021_01_07_425794 84 66 fold fold NN 10_1101-2021_01_07_425794 84 67 - - HYPH 10_1101-2021_01_07_425794 84 68 change change NN 10_1101-2021_01_07_425794 84 69 for for IN 10_1101-2021_01_07_425794 84 70 C c NN 10_1101-2021_01_07_425794 84 71 vs vs IN 10_1101-2021_01_07_425794 84 72 D d NN 10_1101-2021_01_07_425794 84 73 and and CC 10_1101-2021_01_07_425794 84 74 x x NNP 10_1101-2021_01_07_425794 84 75 is be VBZ 10_1101-2021_01_07_425794 84 76 the the DT 10_1101-2021_01_07_425794 84 77 log2 log2 NN 10_1101-2021_01_07_425794 84 78 fold fold NN 10_1101-2021_01_07_425794 84 79 - - HYPH 10_1101-2021_01_07_425794 84 80 change change NN 10_1101-2021_01_07_425794 84 81 for for IN 10_1101-2021_01_07_425794 84 82 A a NN 10_1101-2021_01_07_425794 84 83 vs vs IN 10_1101-2021_01_07_425794 84 84 B. B. NNP 10_1101-2021_01_07_425794 85 1 Expression expression NN 10_1101-2021_01_07_425794 85 2 levels level NNS 10_1101-2021_01_07_425794 85 3 of of IN 10_1101-2021_01_07_425794 85 4 genes gene NNS 10_1101-2021_01_07_425794 85 5 in in IN 10_1101-2021_01_07_425794 85 6 the the DT 10_1101-2021_01_07_425794 85 7 replicates replicate NNS 10_1101-2021_01_07_425794 85 8 of of IN 10_1101-2021_01_07_425794 85 9 the the DT 10_1101-2021_01_07_425794 85 10 same same JJ 10_1101-2021_01_07_425794 85 11 sample sample NN 10_1101-2021_01_07_425794 85 12 were be VBD 10_1101-2021_01_07_425794 85 13 averaged average VBN 10_1101-2021_01_07_425794 85 14 before before IN 10_1101-2021_01_07_425794 85 15 fold fold JJ 10_1101-2021_01_07_425794 85 16 change change NN 10_1101-2021_01_07_425794 85 17 of of IN 10_1101-2021_01_07_425794 85 18 gene gene NN 10_1101-2021_01_07_425794 85 19 expression expression NN 10_1101-2021_01_07_425794 85 20 was be VBD 10_1101-2021_01_07_425794 85 21 calculated calculate VBN 10_1101-2021_01_07_425794 85 22 between between IN 10_1101-2021_01_07_425794 85 23 samples sample NNS 10_1101-2021_01_07_425794 85 24 . . . 10_1101-2021_01_07_425794 86 1 2.5 2.5 CD 10_1101-2021_01_07_425794 86 2 Validation validation NN 10_1101-2021_01_07_425794 86 3 Gene Gene NNP 10_1101-2021_01_07_425794 86 4 expression expression NN 10_1101-2021_01_07_425794 86 5 data datum NNS 10_1101-2021_01_07_425794 86 6 generated generate VBN 10_1101-2021_01_07_425794 86 7 using use VBG 10_1101-2021_01_07_425794 86 8 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 86 9 RT RT NNP 10_1101-2021_01_07_425794 86 10 - - HYPH 10_1101-2021_01_07_425794 86 11 PCR PCR NNP 10_1101-2021_01_07_425794 86 12 and and CC 10_1101-2021_01_07_425794 86 13 Illumina Illumina NNP 10_1101-2021_01_07_425794 86 14 ’s ’s , 10_1101-2021_01_07_425794 86 15 BeadChip BeadChip NNP 10_1101-2021_01_07_425794 86 16 mi- mi- XX 10_1101-2021_01_07_425794 86 17 croarray croarray NN 10_1101-2021_01_07_425794 86 18 were be VBD 10_1101-2021_01_07_425794 86 19 used use VBN 10_1101-2021_01_07_425794 86 20 to to TO 10_1101-2021_01_07_425794 86 21 validate validate VB 10_1101-2021_01_07_425794 86 22 the the DT 10_1101-2021_01_07_425794 86 23 gene gene NN 10_1101-2021_01_07_425794 86 24 - - HYPH 10_1101-2021_01_07_425794 86 25 level level NN 10_1101-2021_01_07_425794 86 26 quantification quantification NN 10_1101-2021_01_07_425794 86 27 results result NNS 10_1101-2021_01_07_425794 86 28 from from IN 10_1101-2021_01_07_425794 86 29 the the DT 10_1101-2021_01_07_425794 86 30 RNA RNA NNP 10_1101-2021_01_07_425794 86 31 - - HYPH 10_1101-2021_01_07_425794 86 32 seq seq NN 10_1101-2021_01_07_425794 86 33 analysis analysis NN 10_1101-2021_01_07_425794 86 34 . . . 10_1101-2021_01_07_425794 87 1 Pearson pearson JJ 10_1101-2021_01_07_425794 87 2 correlation correlation NN 10_1101-2021_01_07_425794 87 3 coefficients coefficient NNS 10_1101-2021_01_07_425794 87 4 were be VBD 10_1101-2021_01_07_425794 87 5 computed compute VBN 10_1101-2021_01_07_425794 87 6 to to TO 10_1101-2021_01_07_425794 87 7 assess assess VB 10_1101-2021_01_07_425794 87 8 the the DT 10_1101-2021_01_07_425794 87 9 concordance concordance NN 10_1101-2021_01_07_425794 87 10 be- be- XX 10_1101-2021_01_07_425794 87 11 tween tween NN 10_1101-2021_01_07_425794 87 12 the the DT 10_1101-2021_01_07_425794 87 13 RNA RNA NNP 10_1101-2021_01_07_425794 87 14 - - HYPH 10_1101-2021_01_07_425794 87 15 seq seq NN 10_1101-2021_01_07_425794 87 16 quantification quantification NN 10_1101-2021_01_07_425794 87 17 data datum NNS 10_1101-2021_01_07_425794 87 18 obtained obtain VBN 10_1101-2021_01_07_425794 87 19 from from IN 10_1101-2021_01_07_425794 87 20 using use VBG 10_1101-2021_01_07_425794 87 21 different different JJ 10_1101-2021_01_07_425794 87 22 annotations annotation NNS 10_1101-2021_01_07_425794 87 23 and and CC 10_1101-2021_01_07_425794 87 24 the the DT 10_1101-2021_01_07_425794 87 25 gene gene NN 10_1101-2021_01_07_425794 87 26 expression expression NN 10_1101-2021_01_07_425794 87 27 data datum NNS 10_1101-2021_01_07_425794 87 28 obtained obtain VBN 10_1101-2021_01_07_425794 87 29 from from IN 10_1101-2021_01_07_425794 87 30 the the DT 10_1101-2021_01_07_425794 87 31 RT RT NNP 10_1101-2021_01_07_425794 87 32 - - HYPH 10_1101-2021_01_07_425794 87 33 PCR PCR NNP 10_1101-2021_01_07_425794 87 34 and and CC 10_1101-2021_01_07_425794 87 35 microarray microarray JJ 10_1101-2021_01_07_425794 87 36 experiments experiment NNS 10_1101-2021_01_07_425794 87 37 . . . 10_1101-2021_01_07_425794 88 1 The the DT 10_1101-2021_01_07_425794 88 2 genome genome NN 10_1101-2021_01_07_425794 88 3 - - HYPH 10_1101-2021_01_07_425794 88 4 wide wide JJ 10_1101-2021_01_07_425794 88 5 built build VBN 10_1101-2021_01_07_425794 88 6 - - HYPH 10_1101-2021_01_07_425794 88 7 in in RP 10_1101-2021_01_07_425794 88 8 truth truth NN 10_1101-2021_01_07_425794 88 9 of of IN 10_1101-2021_01_07_425794 88 10 titration titration NN 10_1101-2021_01_07_425794 88 11 monotonicity monotonicity NN 10_1101-2021_01_07_425794 88 12 of of IN 10_1101-2021_01_07_425794 88 13 gene gene NN 10_1101-2021_01_07_425794 88 14 expression expression NN 10_1101-2021_01_07_425794 88 15 in in IN 10_1101-2021_01_07_425794 88 16 the the DT 10_1101-2021_01_07_425794 88 17 RNA RNA NNP 10_1101-2021_01_07_425794 88 18 - - HYPH 10_1101-2021_01_07_425794 88 19 seq seq NN 10_1101-2021_01_07_425794 88 20 data datum NNS 10_1101-2021_01_07_425794 88 21 was be VBD 10_1101-2021_01_07_425794 88 22 also also RB 10_1101-2021_01_07_425794 88 23 utilized utilize VBN 10_1101-2021_01_07_425794 88 24 to to TO 10_1101-2021_01_07_425794 88 25 evaluate evaluate VB 10_1101-2021_01_07_425794 88 26 the the DT 10_1101-2021_01_07_425794 88 27 quantification quantification NN 10_1101-2021_01_07_425794 88 28 accuracy accuracy NN 10_1101-2021_01_07_425794 88 29 of of IN 10_1101-2021_01_07_425794 88 30 RNA RNA NNP 10_1101-2021_01_07_425794 88 31 - - HYPH 10_1101-2021_01_07_425794 88 32 seq seq NN 10_1101-2021_01_07_425794 88 33 data datum NNS 10_1101-2021_01_07_425794 88 34 generated generate VBN 10_1101-2021_01_07_425794 88 35 from from IN 10_1101-2021_01_07_425794 88 36 using use VBG 10_1101-2021_01_07_425794 88 37 different different JJ 10_1101-2021_01_07_425794 88 38 annotations annotation NNS 10_1101-2021_01_07_425794 88 39 . . . 10_1101-2021_01_07_425794 89 1 2.6 2.6 CD 10_1101-2021_01_07_425794 89 2 Access Access NNP 10_1101-2021_01_07_425794 89 3 to to IN 10_1101-2021_01_07_425794 89 4 data datum NNS 10_1101-2021_01_07_425794 89 5 and and CC 10_1101-2021_01_07_425794 89 6 code code NN 10_1101-2021_01_07_425794 89 7 The the DT 10_1101-2021_01_07_425794 89 8 data datum NNS 10_1101-2021_01_07_425794 89 9 and and CC 10_1101-2021_01_07_425794 89 10 analysis analysis NN 10_1101-2021_01_07_425794 89 11 code code NN 10_1101-2021_01_07_425794 89 12 used use VBN 10_1101-2021_01_07_425794 89 13 in in IN 10_1101-2021_01_07_425794 89 14 this this DT 10_1101-2021_01_07_425794 89 15 study study NN 10_1101-2021_01_07_425794 89 16 can can MD 10_1101-2021_01_07_425794 89 17 be be VB 10_1101-2021_01_07_425794 89 18 accessed access VBN 10_1101-2021_01_07_425794 89 19 at at IN 10_1101-2021_01_07_425794 89 20 the the DT 10_1101-2021_01_07_425794 89 21 following following JJ 10_1101-2021_01_07_425794 89 22 URL url NN 10_1101-2021_01_07_425794 89 23 : : : 10_1101-2021_01_07_425794 89 24 https://github.com/ShiLab-Bioinformatics/GeneAnnotation https://github.com/shilab-bioinformatics/geneannotation NN 10_1101-2021_01_07_425794 89 25 . . . 10_1101-2021_01_07_425794 90 1 6 6 CD 10_1101-2021_01_07_425794 90 2 .CC .CC : 10_1101-2021_01_07_425794 90 3 - - HYPH 10_1101-2021_01_07_425794 90 4 BY by IN 10_1101-2021_01_07_425794 90 5 - - HYPH 10_1101-2021_01_07_425794 90 6 NC NC NNP 10_1101-2021_01_07_425794 90 7 - - HYPH 10_1101-2021_01_07_425794 90 8 ND ND NNP 10_1101-2021_01_07_425794 90 9 4.0 4.0 CD 10_1101-2021_01_07_425794 90 10 International international JJ 10_1101-2021_01_07_425794 90 11 licensemade licensemade NN 10_1101-2021_01_07_425794 90 12 available available JJ 10_1101-2021_01_07_425794 90 13 under under IN 10_1101-2021_01_07_425794 90 14 a a DT 10_1101-2021_01_07_425794 90 15 ( ( -LRB- 10_1101-2021_01_07_425794 90 16 which which WDT 10_1101-2021_01_07_425794 90 17 was be VBD 10_1101-2021_01_07_425794 90 18 not not RB 10_1101-2021_01_07_425794 90 19 certified certify VBN 10_1101-2021_01_07_425794 90 20 by by IN 10_1101-2021_01_07_425794 90 21 peer peer NN 10_1101-2021_01_07_425794 90 22 review review NN 10_1101-2021_01_07_425794 90 23 ) ) -RRB- 10_1101-2021_01_07_425794 90 24 is be VBZ 10_1101-2021_01_07_425794 90 25 the the DT 10_1101-2021_01_07_425794 90 26 author author NN 10_1101-2021_01_07_425794 90 27 / / SYM 10_1101-2021_01_07_425794 90 28 funder funder NN 10_1101-2021_01_07_425794 90 29 , , , 10_1101-2021_01_07_425794 90 30 who who WP 10_1101-2021_01_07_425794 90 31 has have VBZ 10_1101-2021_01_07_425794 90 32 granted grant VBN 10_1101-2021_01_07_425794 90 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 90 34 a a DT 10_1101-2021_01_07_425794 90 35 license license NN 10_1101-2021_01_07_425794 90 36 to to TO 10_1101-2021_01_07_425794 90 37 display display VB 10_1101-2021_01_07_425794 90 38 the the DT 10_1101-2021_01_07_425794 90 39 preprint preprint NN 10_1101-2021_01_07_425794 90 40 in in IN 10_1101-2021_01_07_425794 90 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 90 42 . . . 10_1101-2021_01_07_425794 91 1 It -PRON- PRP 10_1101-2021_01_07_425794 91 2 is be VBZ 10_1101-2021_01_07_425794 91 3 The the DT 10_1101-2021_01_07_425794 91 4 copyright copyright NN 10_1101-2021_01_07_425794 91 5 holder holder NN 10_1101-2021_01_07_425794 91 6 for for IN 10_1101-2021_01_07_425794 91 7 this this DT 10_1101-2021_01_07_425794 91 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 91 9 version version NN 10_1101-2021_01_07_425794 91 10 posted post VBD 10_1101-2021_01_07_425794 91 11 January January NNP 10_1101-2021_01_07_425794 91 12 8 8 CD 10_1101-2021_01_07_425794 91 13 , , , 10_1101-2021_01_07_425794 91 14 2021 2021 CD 10_1101-2021_01_07_425794 91 15 . . . 10_1101-2021_01_07_425794 91 16 ; ; : 10_1101-2021_01_07_425794 91 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 91 18 : : : 10_1101-2021_01_07_425794 91 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 91 20 preprint preprint JJ 10_1101-2021_01_07_425794 91 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 91 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 91 23 3 3 CD 10_1101-2021_01_07_425794 91 24 RESULTS RESULTS NNP 10_1101-2021_01_07_425794 91 25 3.1 3.1 CD 10_1101-2021_01_07_425794 91 26 Discrepancy discrepancy NN 10_1101-2021_01_07_425794 91 27 between between IN 10_1101-2021_01_07_425794 91 28 different different JJ 10_1101-2021_01_07_425794 91 29 gene gene NN 10_1101-2021_01_07_425794 91 30 annotations annotation NNS 10_1101-2021_01_07_425794 91 31 The the DT 10_1101-2021_01_07_425794 91 32 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 91 33 and and CC 10_1101-2021_01_07_425794 91 34 NCBI NCBI NNP 10_1101-2021_01_07_425794 91 35 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 91 36 annotations annotation NNS 10_1101-2021_01_07_425794 91 37 are be VBP 10_1101-2021_01_07_425794 91 38 among among IN 10_1101-2021_01_07_425794 91 39 the the DT 10_1101-2021_01_07_425794 91 40 most most RBS 10_1101-2021_01_07_425794 91 41 widely widely RB 10_1101-2021_01_07_425794 91 42 used use VBN 10_1101-2021_01_07_425794 91 43 gene gene NN 10_1101-2021_01_07_425794 91 44 anno- anno- NN 10_1101-2021_01_07_425794 91 45 tations tation NNS 10_1101-2021_01_07_425794 91 46 that that WDT 10_1101-2021_01_07_425794 91 47 have have VBP 10_1101-2021_01_07_425794 91 48 been be VBN 10_1101-2021_01_07_425794 91 49 utilized utilize VBN 10_1101-2021_01_07_425794 91 50 for for IN 10_1101-2021_01_07_425794 91 51 RNA RNA NNP 10_1101-2021_01_07_425794 91 52 - - HYPH 10_1101-2021_01_07_425794 91 53 seq seq NN 10_1101-2021_01_07_425794 91 54 gene gene NN 10_1101-2021_01_07_425794 91 55 expression expression NN 10_1101-2021_01_07_425794 91 56 quantification quantification NN 10_1101-2021_01_07_425794 91 57 in in IN 10_1101-2021_01_07_425794 91 58 the the DT 10_1101-2021_01_07_425794 91 59 field field NN 10_1101-2021_01_07_425794 91 60 . . . 10_1101-2021_01_07_425794 92 1 In in IN 10_1101-2021_01_07_425794 92 2 this this DT 10_1101-2021_01_07_425794 92 3 study study NN 10_1101-2021_01_07_425794 92 4 , , , 10_1101-2021_01_07_425794 92 5 we -PRON- PRP 10_1101-2021_01_07_425794 92 6 downloaded download VBD 10_1101-2021_01_07_425794 92 7 recent recent JJ 10_1101-2021_01_07_425794 92 8 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 92 9 and and CC 10_1101-2021_01_07_425794 92 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 92 11 annotations annotation NNS 10_1101-2021_01_07_425794 92 12 and and CC 10_1101-2021_01_07_425794 92 13 also also RB 10_1101-2021_01_07_425794 92 14 used use VBD 10_1101-2021_01_07_425794 92 15 an an DT 10_1101-2021_01_07_425794 92 16 older old JJR 10_1101-2021_01_07_425794 92 17 version version NN 10_1101-2021_01_07_425794 92 18 of of IN 10_1101-2021_01_07_425794 92 19 Refseq Refseq NNP 10_1101-2021_01_07_425794 92 20 annotation annotation NN 10_1101-2021_01_07_425794 92 21 to to TO 10_1101-2021_01_07_425794 92 22 assess assess VB 10_1101-2021_01_07_425794 92 23 the the DT 10_1101-2021_01_07_425794 92 24 impact impact NN 10_1101-2021_01_07_425794 92 25 of of IN 10_1101-2021_01_07_425794 92 26 gene gene NN 10_1101-2021_01_07_425794 92 27 annotation annotation NN 10_1101-2021_01_07_425794 92 28 choice choice NN 10_1101-2021_01_07_425794 92 29 on on IN 10_1101-2021_01_07_425794 92 30 the the DT 10_1101-2021_01_07_425794 92 31 accuracy accuracy NN 10_1101-2021_01_07_425794 92 32 of of IN 10_1101-2021_01_07_425794 92 33 RNA RNA NNP 10_1101-2021_01_07_425794 92 34 - - HYPH 10_1101-2021_01_07_425794 92 35 seq seq NN 10_1101-2021_01_07_425794 92 36 expression expression NN 10_1101-2021_01_07_425794 92 37 quantification quantification NN 10_1101-2021_01_07_425794 92 38 . . . 10_1101-2021_01_07_425794 93 1 The the DT 10_1101-2021_01_07_425794 93 2 inclusion inclusion NN 10_1101-2021_01_07_425794 93 3 of of IN 10_1101-2021_01_07_425794 93 4 an an DT 10_1101-2021_01_07_425794 93 5 older old JJR 10_1101-2021_01_07_425794 93 6 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 93 7 annotation annotation NN 10_1101-2021_01_07_425794 93 8 allowed allow VBD 10_1101-2021_01_07_425794 93 9 us -PRON- PRP 10_1101-2021_01_07_425794 93 10 to to TO 10_1101-2021_01_07_425794 93 11 investigate investigate VB 10_1101-2021_01_07_425794 93 12 the the DT 10_1101-2021_01_07_425794 93 13 accuracy accuracy NN 10_1101-2021_01_07_425794 93 14 of of IN 10_1101-2021_01_07_425794 93 15 new new JJ 10_1101-2021_01_07_425794 93 16 annotation annotation NN 10_1101-2021_01_07_425794 93 17 data datum NNS 10_1101-2021_01_07_425794 93 18 generated generate VBN 10_1101-2021_01_07_425794 93 19 in in IN 10_1101-2021_01_07_425794 93 20 recent recent JJ 10_1101-2021_01_07_425794 93 21 years year NNS 10_1101-2021_01_07_425794 93 22 when when WRB 10_1101-2021_01_07_425794 93 23 the the DT 10_1101-2021_01_07_425794 93 24 next next JJ 10_1101-2021_01_07_425794 93 25 - - HYPH 10_1101-2021_01_07_425794 93 26 gen gen NNP 10_1101-2021_01_07_425794 93 27 sequencing sequencing NNP 10_1101-2021_01_07_425794 93 28 data datum NNS 10_1101-2021_01_07_425794 93 29 have have VBP 10_1101-2021_01_07_425794 93 30 been be VBN 10_1101-2021_01_07_425794 93 31 used use VBN 10_1101-2021_01_07_425794 93 32 as as IN 10_1101-2021_01_07_425794 93 33 a a DT 10_1101-2021_01_07_425794 93 34 new new JJ 10_1101-2021_01_07_425794 93 35 data data NN 10_1101-2021_01_07_425794 93 36 source source NN 10_1101-2021_01_07_425794 93 37 for for IN 10_1101-2021_01_07_425794 93 38 genome genome NN 10_1101-2021_01_07_425794 93 39 - - HYPH 10_1101-2021_01_07_425794 93 40 wide wide JJ 10_1101-2021_01_07_425794 93 41 annotation annotation NN 10_1101-2021_01_07_425794 93 42 generation generation NN 10_1101-2021_01_07_425794 93 43 . . . 10_1101-2021_01_07_425794 94 1 The the DT 10_1101-2021_01_07_425794 94 2 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 94 3 annotation annotation NN 10_1101-2021_01_07_425794 94 4 used use VBN 10_1101-2021_01_07_425794 94 5 in in IN 10_1101-2021_01_07_425794 94 6 this this DT 10_1101-2021_01_07_425794 94 7 study study NN 10_1101-2021_01_07_425794 94 8 was be VBD 10_1101-2021_01_07_425794 94 9 released release VBN 10_1101-2021_01_07_425794 94 10 in in IN 10_1101-2021_01_07_425794 94 11 April April NNP 10_1101-2021_01_07_425794 94 12 2020 2020 CD 10_1101-2021_01_07_425794 94 13 and and CC 10_1101-2021_01_07_425794 94 14 it -PRON- PRP 10_1101-2021_01_07_425794 94 15 has have VBZ 10_1101-2021_01_07_425794 94 16 a a DT 10_1101-2021_01_07_425794 94 17 version version NN 10_1101-2021_01_07_425794 94 18 number number NN 10_1101-2021_01_07_425794 94 19 100 100 CD 10_1101-2021_01_07_425794 94 20 . . . 10_1101-2021_01_07_425794 95 1 The the DT 10_1101-2021_01_07_425794 95 2 recent recent JJ 10_1101-2021_01_07_425794 95 3 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 95 4 annotation annotation NN 10_1101-2021_01_07_425794 95 5 included include VBN 10_1101-2021_01_07_425794 95 6 in in IN 10_1101-2021_01_07_425794 95 7 this this DT 10_1101-2021_01_07_425794 95 8 study study NN 10_1101-2021_01_07_425794 95 9 was be VBD 10_1101-2021_01_07_425794 95 10 released release VBN 10_1101-2021_01_07_425794 95 11 in in IN 10_1101-2021_01_07_425794 95 12 August August NNP 10_1101-2021_01_07_425794 95 13 2020 2020 CD 10_1101-2021_01_07_425794 95 14 . . . 10_1101-2021_01_07_425794 96 1 We -PRON- PRP 10_1101-2021_01_07_425794 96 2 call call VBP 10_1101-2021_01_07_425794 96 3 this this DT 10_1101-2021_01_07_425794 96 4 annotation annotation NN 10_1101-2021_01_07_425794 96 5 as as IN 10_1101-2021_01_07_425794 96 6 ‘ ' `` 10_1101-2021_01_07_425794 96 7 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 96 8 - - HYPH 10_1101-2021_01_07_425794 96 9 NCBI NCBI NNP 10_1101-2021_01_07_425794 96 10 ’ ' '' 10_1101-2021_01_07_425794 96 11 in in IN 10_1101-2021_01_07_425794 96 12 this this DT 10_1101-2021_01_07_425794 96 13 study study NN 10_1101-2021_01_07_425794 96 14 . . . 10_1101-2021_01_07_425794 97 1 The the DT 10_1101-2021_01_07_425794 97 2 older old JJR 10_1101-2021_01_07_425794 97 3 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 97 4 annotation annotation NN 10_1101-2021_01_07_425794 97 5 was be VBD 10_1101-2021_01_07_425794 97 6 released release VBN 10_1101-2021_01_07_425794 97 7 in in IN 10_1101-2021_01_07_425794 97 8 April April NNP 10_1101-2021_01_07_425794 97 9 2015 2015 CD 10_1101-2021_01_07_425794 97 10 , , , 10_1101-2021_01_07_425794 97 11 and and CC 10_1101-2021_01_07_425794 97 12 it -PRON- PRP 10_1101-2021_01_07_425794 97 13 has have VBZ 10_1101-2021_01_07_425794 97 14 also also RB 10_1101-2021_01_07_425794 97 15 been be VBN 10_1101-2021_01_07_425794 97 16 included include VBN 10_1101-2021_01_07_425794 97 17 as as IN 10_1101-2021_01_07_425794 97 18 part part NN 10_1101-2021_01_07_425794 97 19 of of IN 10_1101-2021_01_07_425794 97 20 the the DT 10_1101-2021_01_07_425794 97 21 popular popular JJ 10_1101-2021_01_07_425794 97 22 RNA RNA NNP 10_1101-2021_01_07_425794 97 23 - - HYPH 10_1101-2021_01_07_425794 97 24 seq seq NN 10_1101-2021_01_07_425794 97 25 quantification quantification NN 10_1101-2021_01_07_425794 97 26 toolkit toolkit NNS 10_1101-2021_01_07_425794 97 27 ‘ ' `` 10_1101-2021_01_07_425794 97 28 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 97 29 ’ ' '' 10_1101-2021_01_07_425794 97 30 for for IN 10_1101-2021_01_07_425794 97 31 quantifying quantify VBG 10_1101-2021_01_07_425794 97 32 human human JJ 10_1101-2021_01_07_425794 97 33 RNA- RNA- NNP 10_1101-2021_01_07_425794 97 34 seq seq NN 10_1101-2021_01_07_425794 97 35 data datum NNS 10_1101-2021_01_07_425794 97 36 . . . 10_1101-2021_01_07_425794 98 1 As as IN 10_1101-2021_01_07_425794 98 2 this this DT 10_1101-2021_01_07_425794 98 3 annotation annotation NN 10_1101-2021_01_07_425794 98 4 is be VBZ 10_1101-2021_01_07_425794 98 5 not not RB 10_1101-2021_01_07_425794 98 6 available available JJ 10_1101-2021_01_07_425794 98 7 in in IN 10_1101-2021_01_07_425794 98 8 the the DT 10_1101-2021_01_07_425794 98 9 NCBI NCBI NNP 10_1101-2021_01_07_425794 98 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 98 11 database database NN 10_1101-2021_01_07_425794 98 12 anymore anymore RB 10_1101-2021_01_07_425794 98 13 , , , 10_1101-2021_01_07_425794 98 14 we -PRON- PRP 10_1101-2021_01_07_425794 98 15 instead instead RB 10_1101-2021_01_07_425794 98 16 used use VBD 10_1101-2021_01_07_425794 98 17 the the DT 10_1101-2021_01_07_425794 98 18 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 98 19 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 98 20 annotation annotation NN 10_1101-2021_01_07_425794 98 21 in in IN 10_1101-2021_01_07_425794 98 22 our -PRON- PRP$ 10_1101-2021_01_07_425794 98 23 evaluations evaluation NNS 10_1101-2021_01_07_425794 98 24 and and CC 10_1101-2021_01_07_425794 98 25 we -PRON- PRP 10_1101-2021_01_07_425794 98 26 denote denote VBP 10_1101-2021_01_07_425794 98 27 this this DT 10_1101-2021_01_07_425794 98 28 annotation annotation NN 10_1101-2021_01_07_425794 98 29 as as IN 10_1101-2021_01_07_425794 98 30 ‘ ' `` 10_1101-2021_01_07_425794 98 31 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 98 32 - - HYPH 10_1101-2021_01_07_425794 98 33 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 98 34 ’ ' '' 10_1101-2021_01_07_425794 98 35 . . . 10_1101-2021_01_07_425794 99 1 As as IN 10_1101-2021_01_07_425794 99 2 RNA RNA NNP 10_1101-2021_01_07_425794 99 3 - - HYPH 10_1101-2021_01_07_425794 99 4 seq seq NN 10_1101-2021_01_07_425794 99 5 gene gene NN 10_1101-2021_01_07_425794 99 6 - - HYPH 10_1101-2021_01_07_425794 99 7 level level NN 10_1101-2021_01_07_425794 99 8 expression expression NN 10_1101-2021_01_07_425794 99 9 quantification quantification NN 10_1101-2021_01_07_425794 99 10 is be VBZ 10_1101-2021_01_07_425794 99 11 typically typically RB 10_1101-2021_01_07_425794 99 12 performed perform VBN 10_1101-2021_01_07_425794 99 13 for for IN 10_1101-2021_01_07_425794 99 14 genes gene NNS 10_1101-2021_01_07_425794 99 15 that that WDT 10_1101-2021_01_07_425794 99 16 contain contain VBP 10_1101-2021_01_07_425794 99 17 exons exon NNS 10_1101-2021_01_07_425794 99 18 [ [ -LRB- 10_1101-2021_01_07_425794 99 19 3 3 CD 10_1101-2021_01_07_425794 99 20 , , , 10_1101-2021_01_07_425794 99 21 4 4 CD 10_1101-2021_01_07_425794 99 22 , , , 10_1101-2021_01_07_425794 99 23 5 5 CD 10_1101-2021_01_07_425794 99 24 ] ] -RRB- 10_1101-2021_01_07_425794 99 25 , , , 10_1101-2021_01_07_425794 99 26 in in IN 10_1101-2021_01_07_425794 99 27 this this DT 10_1101-2021_01_07_425794 99 28 study study NN 10_1101-2021_01_07_425794 99 29 we -PRON- PRP 10_1101-2021_01_07_425794 99 30 only only RB 10_1101-2021_01_07_425794 99 31 focused focus VBD 10_1101-2021_01_07_425794 99 32 on on IN 10_1101-2021_01_07_425794 99 33 the the DT 10_1101-2021_01_07_425794 99 34 genes gene NNS 10_1101-2021_01_07_425794 99 35 that that WDT 10_1101-2021_01_07_425794 99 36 have have VBP 10_1101-2021_01_07_425794 99 37 annotated annotate VBN 10_1101-2021_01_07_425794 99 38 exons exon NNS 10_1101-2021_01_07_425794 99 39 in in IN 10_1101-2021_01_07_425794 99 40 each each DT 10_1101-2021_01_07_425794 99 41 annotation annotation NN 10_1101-2021_01_07_425794 99 42 . . . 10_1101-2021_01_07_425794 100 1 Figure figure NN 10_1101-2021_01_07_425794 100 2 1A 1a NN 10_1101-2021_01_07_425794 100 3 shows show VBZ 10_1101-2021_01_07_425794 100 4 that that IN 10_1101-2021_01_07_425794 100 5 , , , 10_1101-2021_01_07_425794 100 6 as as IN 10_1101-2021_01_07_425794 100 7 expected expect VBN 10_1101-2021_01_07_425794 100 8 , , , 10_1101-2021_01_07_425794 100 9 the the DT 10_1101-2021_01_07_425794 100 10 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 100 11 annotation annotation NN 10_1101-2021_01_07_425794 100 12 contains contain VBZ 10_1101-2021_01_07_425794 100 13 a a DT 10_1101-2021_01_07_425794 100 14 lot lot NN 10_1101-2021_01_07_425794 100 15 more more RBR 10_1101-2021_01_07_425794 100 16 exon exon NN 10_1101-2021_01_07_425794 100 17 - - HYPH 10_1101-2021_01_07_425794 100 18 containing contain VBG 10_1101-2021_01_07_425794 100 19 genes gene NNS 10_1101-2021_01_07_425794 100 20 than than IN 10_1101-2021_01_07_425794 100 21 the the DT 10_1101-2021_01_07_425794 100 22 two two CD 10_1101-2021_01_07_425794 100 23 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 100 24 annotations annotation NNS 10_1101-2021_01_07_425794 100 25 . . . 10_1101-2021_01_07_425794 101 1 The the DT 10_1101-2021_01_07_425794 101 2 En- en- CD 10_1101-2021_01_07_425794 101 3 sembl sembl NN 10_1101-2021_01_07_425794 101 4 annotation annotation NN 10_1101-2021_01_07_425794 101 5 is be VBZ 10_1101-2021_01_07_425794 101 6 known know VBN 10_1101-2021_01_07_425794 101 7 to to TO 10_1101-2021_01_07_425794 101 8 contain contain VB 10_1101-2021_01_07_425794 101 9 a a DT 10_1101-2021_01_07_425794 101 10 large large JJ 10_1101-2021_01_07_425794 101 11 number number NN 10_1101-2021_01_07_425794 101 12 of of IN 10_1101-2021_01_07_425794 101 13 computationally computationally RB 10_1101-2021_01_07_425794 101 14 predicted predict VBN 10_1101-2021_01_07_425794 101 15 genes gene NNS 10_1101-2021_01_07_425794 101 16 whereas whereas IN 10_1101-2021_01_07_425794 101 17 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 101 18 genes gene NNS 10_1101-2021_01_07_425794 101 19 were be VBD 10_1101-2021_01_07_425794 101 20 mainly mainly RB 10_1101-2021_01_07_425794 101 21 annotated annotate VBN 10_1101-2021_01_07_425794 101 22 based base VBN 10_1101-2021_01_07_425794 101 23 on on IN 10_1101-2021_01_07_425794 101 24 the the DT 10_1101-2021_01_07_425794 101 25 biological biological JJ 10_1101-2021_01_07_425794 101 26 evidence evidence NN 10_1101-2021_01_07_425794 101 27 . . . 10_1101-2021_01_07_425794 102 1 However however RB 10_1101-2021_01_07_425794 102 2 , , , 10_1101-2021_01_07_425794 102 3 it -PRON- PRP 10_1101-2021_01_07_425794 102 4 is be VBZ 10_1101-2021_01_07_425794 102 5 worth worth JJ 10_1101-2021_01_07_425794 102 6 noting note VBG 10_1101-2021_01_07_425794 102 7 that that IN 10_1101-2021_01_07_425794 102 8 the the DT 10_1101-2021_01_07_425794 102 9 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 102 10 - - HYPH 10_1101-2021_01_07_425794 102 11 NCBI NCBI NNP 10_1101-2021_01_07_425794 102 12 annotation annotation NN 10_1101-2021_01_07_425794 102 13 still still RB 10_1101-2021_01_07_425794 102 14 has have VBZ 10_1101-2021_01_07_425794 102 15 > > XX 10_1101-2021_01_07_425794 102 16 14,000 14,000 CD 10_1101-2021_01_07_425794 102 17 genes gene NNS 10_1101-2021_01_07_425794 102 18 that that WDT 10_1101-2021_01_07_425794 102 19 are be VBP 10_1101-2021_01_07_425794 102 20 not not RB 10_1101-2021_01_07_425794 102 21 included include VBN 10_1101-2021_01_07_425794 102 22 in in IN 10_1101-2021_01_07_425794 102 23 the the DT 10_1101-2021_01_07_425794 102 24 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 102 25 annotation annotation NN 10_1101-2021_01_07_425794 102 26 . . . 10_1101-2021_01_07_425794 103 1 Nearly nearly RB 10_1101-2021_01_07_425794 103 2 60 60 CD 10_1101-2021_01_07_425794 103 3 % % NN 10_1101-2021_01_07_425794 103 4 of of IN 10_1101-2021_01_07_425794 103 5 the the DT 10_1101-2021_01_07_425794 103 6 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 103 7 genes gene NNS 10_1101-2021_01_07_425794 103 8 were be VBD 10_1101-2021_01_07_425794 103 9 found find VBN 10_1101-2021_01_07_425794 103 10 to to TO 10_1101-2021_01_07_425794 103 11 be be VB 10_1101-2021_01_07_425794 103 12 absent absent JJ 10_1101-2021_01_07_425794 103 13 from from IN 10_1101-2021_01_07_425794 103 14 both both DT 10_1101-2021_01_07_425794 103 15 of of IN 10_1101-2021_01_07_425794 103 16 the the DT 10_1101-2021_01_07_425794 103 17 two two CD 10_1101-2021_01_07_425794 103 18 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 103 19 annotations annotation NNS 10_1101-2021_01_07_425794 103 20 . . . 10_1101-2021_01_07_425794 104 1 In in IN 10_1101-2021_01_07_425794 104 2 total total JJ 10_1101-2021_01_07_425794 104 3 , , , 10_1101-2021_01_07_425794 104 4 23,424 23,424 CD 10_1101-2021_01_07_425794 104 5 common common JJ 10_1101-2021_01_07_425794 104 6 genes gene NNS 10_1101-2021_01_07_425794 104 7 were be VBD 10_1101-2021_01_07_425794 104 8 found find VBN 10_1101-2021_01_07_425794 104 9 between between IN 10_1101-2021_01_07_425794 104 10 the the DT 10_1101-2021_01_07_425794 104 11 three three CD 10_1101-2021_01_07_425794 104 12 annotations annotation NNS 10_1101-2021_01_07_425794 104 13 . . . 10_1101-2021_01_07_425794 105 1 Most Most JJS 10_1101-2021_01_07_425794 105 2 of of IN 10_1101-2021_01_07_425794 105 3 the the DT 10_1101-2021_01_07_425794 105 4 genes gene NNS 10_1101-2021_01_07_425794 105 5 included include VBN 10_1101-2021_01_07_425794 105 6 in in IN 10_1101-2021_01_07_425794 105 7 the the DT 10_1101-2021_01_07_425794 105 8 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 105 9 - - HYPH 10_1101-2021_01_07_425794 105 10 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 105 11 annotation annotation NN 10_1101-2021_01_07_425794 105 12 can can MD 10_1101-2021_01_07_425794 105 13 be be VB 10_1101-2021_01_07_425794 105 14 found find VBN 10_1101-2021_01_07_425794 105 15 in in IN 10_1101-2021_01_07_425794 105 16 the the DT 10_1101-2021_01_07_425794 105 17 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 105 18 - - HYPH 10_1101-2021_01_07_425794 105 19 NCBI NCBI NNP 10_1101-2021_01_07_425794 105 20 or or CC 10_1101-2021_01_07_425794 105 21 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 105 22 annotations annotation NNS 10_1101-2021_01_07_425794 105 23 . . . 10_1101-2021_01_07_425794 106 1 We -PRON- PRP 10_1101-2021_01_07_425794 106 2 then then RB 10_1101-2021_01_07_425794 106 3 examined examine VBD 10_1101-2021_01_07_425794 106 4 the the DT 10_1101-2021_01_07_425794 106 5 effective effective JJ 10_1101-2021_01_07_425794 106 6 gene gene NN 10_1101-2021_01_07_425794 106 7 lengths length NNS 10_1101-2021_01_07_425794 106 8 in in IN 10_1101-2021_01_07_425794 106 9 each each DT 10_1101-2021_01_07_425794 106 10 annotation annotation NN 10_1101-2021_01_07_425794 106 11 . . . 10_1101-2021_01_07_425794 107 1 The the DT 10_1101-2021_01_07_425794 107 2 effective effective JJ 10_1101-2021_01_07_425794 107 3 length length NN 10_1101-2021_01_07_425794 107 4 of of IN 10_1101-2021_01_07_425794 107 5 a a DT 10_1101-2021_01_07_425794 107 6 gene gene NN 10_1101-2021_01_07_425794 107 7 is be VBZ 10_1101-2021_01_07_425794 107 8 the the DT 10_1101-2021_01_07_425794 107 9 total total JJ 10_1101-2021_01_07_425794 107 10 number number NN 10_1101-2021_01_07_425794 107 11 of of IN 10_1101-2021_01_07_425794 107 12 unique unique JJ 10_1101-2021_01_07_425794 107 13 bases basis NNS 10_1101-2021_01_07_425794 107 14 included include VBN 10_1101-2021_01_07_425794 107 15 in in IN 10_1101-2021_01_07_425794 107 16 all all PDT 10_1101-2021_01_07_425794 107 17 the the DT 10_1101-2021_01_07_425794 107 18 exons exon NNS 10_1101-2021_01_07_425794 107 19 belonging belong VBG 10_1101-2021_01_07_425794 107 20 to to IN 10_1101-2021_01_07_425794 107 21 the the DT 10_1101-2021_01_07_425794 107 22 gene gene NN 10_1101-2021_01_07_425794 107 23 . . . 10_1101-2021_01_07_425794 108 1 Figure figure NN 10_1101-2021_01_07_425794 108 2 1B 1b NN 10_1101-2021_01_07_425794 108 3 shows show VBZ 10_1101-2021_01_07_425794 108 4 the the DT 10_1101-2021_01_07_425794 108 5 distributions distribution NNS 10_1101-2021_01_07_425794 108 6 of of IN 10_1101-2021_01_07_425794 108 7 effective effective JJ 10_1101-2021_01_07_425794 108 8 lengths length NNS 10_1101-2021_01_07_425794 108 9 of of IN 10_1101-2021_01_07_425794 108 10 genes gene NNS 10_1101-2021_01_07_425794 108 11 in in IN 10_1101-2021_01_07_425794 108 12 the the DT 10_1101-2021_01_07_425794 108 13 three three CD 10_1101-2021_01_07_425794 108 14 annota- annota- JJ 10_1101-2021_01_07_425794 108 15 tions tion NNS 10_1101-2021_01_07_425794 108 16 . . . 10_1101-2021_01_07_425794 109 1 Around around IN 10_1101-2021_01_07_425794 109 2 half half NN 10_1101-2021_01_07_425794 109 3 of of IN 10_1101-2021_01_07_425794 109 4 the the DT 10_1101-2021_01_07_425794 109 5 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 109 6 genes gene NNS 10_1101-2021_01_07_425794 109 7 have have VBP 10_1101-2021_01_07_425794 109 8 an an DT 10_1101-2021_01_07_425794 109 9 effective effective JJ 10_1101-2021_01_07_425794 109 10 length length NN 10_1101-2021_01_07_425794 109 11 less less JJR 10_1101-2021_01_07_425794 109 12 than than IN 10_1101-2021_01_07_425794 109 13 1,000 1,000 CD 10_1101-2021_01_07_425794 109 14 bases basis NNS 10_1101-2021_01_07_425794 109 15 , , , 10_1101-2021_01_07_425794 109 16 whereas whereas IN 10_1101-2021_01_07_425794 109 17 in in IN 10_1101-2021_01_07_425794 109 18 the the DT 10_1101-2021_01_07_425794 109 19 two two CD 10_1101-2021_01_07_425794 109 20 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 109 21 annotations annotation NNS 10_1101-2021_01_07_425794 109 22 only only RB 10_1101-2021_01_07_425794 109 23 ∼25 ∼25 VBP 10_1101-2021_01_07_425794 109 24 % % NN 10_1101-2021_01_07_425794 109 25 of of IN 10_1101-2021_01_07_425794 109 26 the the DT 10_1101-2021_01_07_425794 109 27 genes gene NNS 10_1101-2021_01_07_425794 109 28 are be VBP 10_1101-2021_01_07_425794 109 29 shorter short JJR 10_1101-2021_01_07_425794 109 30 than than IN 10_1101-2021_01_07_425794 109 31 1,000 1,000 CD 10_1101-2021_01_07_425794 109 32 bases basis NNS 10_1101-2021_01_07_425794 109 33 in in IN 10_1101-2021_01_07_425794 109 34 length length NN 10_1101-2021_01_07_425794 109 35 . . . 10_1101-2021_01_07_425794 110 1 The the DT 10_1101-2021_01_07_425794 110 2 median median JJ 10_1101-2021_01_07_425794 110 3 effective effective JJ 10_1101-2021_01_07_425794 110 4 gene gene NN 10_1101-2021_01_07_425794 110 5 lengths length NNS 10_1101-2021_01_07_425794 110 6 in in IN 10_1101-2021_01_07_425794 110 7 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 110 8 - - HYPH 10_1101-2021_01_07_425794 110 9 NCBI NCBI NNP 10_1101-2021_01_07_425794 110 10 and and CC 10_1101-2021_01_07_425794 110 11 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 110 12 - - HYPH 10_1101-2021_01_07_425794 110 13 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 110 14 7 7 CD 10_1101-2021_01_07_425794 110 15 .CC .cc SYM 10_1101-2021_01_07_425794 110 16 - - HYPH 10_1101-2021_01_07_425794 110 17 BY by IN 10_1101-2021_01_07_425794 110 18 - - HYPH 10_1101-2021_01_07_425794 110 19 NC NC NNP 10_1101-2021_01_07_425794 110 20 - - HYPH 10_1101-2021_01_07_425794 110 21 ND ND NNP 10_1101-2021_01_07_425794 110 22 4.0 4.0 CD 10_1101-2021_01_07_425794 110 23 International international JJ 10_1101-2021_01_07_425794 110 24 licensemade licensemade NN 10_1101-2021_01_07_425794 110 25 available available JJ 10_1101-2021_01_07_425794 110 26 under under IN 10_1101-2021_01_07_425794 110 27 a a DT 10_1101-2021_01_07_425794 110 28 ( ( -LRB- 10_1101-2021_01_07_425794 110 29 which which WDT 10_1101-2021_01_07_425794 110 30 was be VBD 10_1101-2021_01_07_425794 110 31 not not RB 10_1101-2021_01_07_425794 110 32 certified certify VBN 10_1101-2021_01_07_425794 110 33 by by IN 10_1101-2021_01_07_425794 110 34 peer peer NN 10_1101-2021_01_07_425794 110 35 review review NN 10_1101-2021_01_07_425794 110 36 ) ) -RRB- 10_1101-2021_01_07_425794 110 37 is be VBZ 10_1101-2021_01_07_425794 110 38 the the DT 10_1101-2021_01_07_425794 110 39 author author NN 10_1101-2021_01_07_425794 110 40 / / SYM 10_1101-2021_01_07_425794 110 41 funder funder NN 10_1101-2021_01_07_425794 110 42 , , , 10_1101-2021_01_07_425794 110 43 who who WP 10_1101-2021_01_07_425794 110 44 has have VBZ 10_1101-2021_01_07_425794 110 45 granted grant VBN 10_1101-2021_01_07_425794 110 46 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 110 47 a a DT 10_1101-2021_01_07_425794 110 48 license license NN 10_1101-2021_01_07_425794 110 49 to to TO 10_1101-2021_01_07_425794 110 50 display display VB 10_1101-2021_01_07_425794 110 51 the the DT 10_1101-2021_01_07_425794 110 52 preprint preprint NN 10_1101-2021_01_07_425794 110 53 in in IN 10_1101-2021_01_07_425794 110 54 perpetuity perpetuity NN 10_1101-2021_01_07_425794 110 55 . . . 10_1101-2021_01_07_425794 111 1 It -PRON- PRP 10_1101-2021_01_07_425794 111 2 is be VBZ 10_1101-2021_01_07_425794 111 3 The the DT 10_1101-2021_01_07_425794 111 4 copyright copyright NN 10_1101-2021_01_07_425794 111 5 holder holder NN 10_1101-2021_01_07_425794 111 6 for for IN 10_1101-2021_01_07_425794 111 7 this this DT 10_1101-2021_01_07_425794 111 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 111 9 version version NN 10_1101-2021_01_07_425794 111 10 posted post VBD 10_1101-2021_01_07_425794 111 11 January January NNP 10_1101-2021_01_07_425794 111 12 8 8 CD 10_1101-2021_01_07_425794 111 13 , , , 10_1101-2021_01_07_425794 111 14 2021 2021 CD 10_1101-2021_01_07_425794 111 15 . . . 10_1101-2021_01_07_425794 111 16 ; ; : 10_1101-2021_01_07_425794 111 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 111 18 : : : 10_1101-2021_01_07_425794 111 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 111 20 preprint preprint JJ 10_1101-2021_01_07_425794 111 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 111 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 111 23 A A NNP 10_1101-2021_01_07_425794 111 24 B B NNP 10_1101-2021_01_07_425794 111 25 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 111 26 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 111 27 - - HYPH 10_1101-2021_01_07_425794 111 28 NCBI NCBI NNP 10_1101-2021_01_07_425794 111 29 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 111 30 - - HYPH 10_1101-2021_01_07_425794 111 31 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 111 32 1,354 1,354 CD 10_1101-2021_01_07_425794 111 33 23,424 23,424 CD 10_1101-2021_01_07_425794 111 34 4,27820 4,27820 CD 10_1101-2021_01_07_425794 111 35 35,885 35,885 CD 10_1101-2021_01_07_425794 111 36 673 673 CD 10_1101-2021_01_07_425794 111 37 10,479 10,479 CD 10_1101-2021_01_07_425794 111 38 E e NN 10_1101-2021_01_07_425794 111 39 ns ns IN 10_1101-2021_01_07_425794 111 40 em -PRON- PRP 10_1101-2021_01_07_425794 111 41 bl bl NNP 10_1101-2021_01_07_425794 111 42 R R NNP 10_1101-2021_01_07_425794 111 43 ef ef NNP 10_1101-2021_01_07_425794 111 44 S S NNP 10_1101-2021_01_07_425794 111 45 eq eq NNP 10_1101-2021_01_07_425794 111 46 -R -R NNP 10_1101-2021_01_07_425794 111 47 su su NNP 10_1101-2021_01_07_425794 111 48 br br VBP 10_1101-2021_01_07_425794 111 49 ea ea NNP 10_1101-2021_01_07_425794 111 50 d d NNP 10_1101-2021_01_07_425794 111 51 Lo Lo NNP 10_1101-2021_01_07_425794 111 52 g2 g2 NNP 10_1101-2021_01_07_425794 111 53 ef ef NNP 10_1101-2021_01_07_425794 111 54 fe fe NNP 10_1101-2021_01_07_425794 111 55 ct ct NNP 10_1101-2021_01_07_425794 111 56 iv iv NNP 10_1101-2021_01_07_425794 111 57 e e NNP 10_1101-2021_01_07_425794 111 58 ge ge NNP 10_1101-2021_01_07_425794 111 59 ne ne NNP 10_1101-2021_01_07_425794 111 60 le le NNP 10_1101-2021_01_07_425794 111 61 ng ng PRP 10_1101-2021_01_07_425794 111 62 th th XX 10_1101-2021_01_07_425794 111 63 R r NN 10_1101-2021_01_07_425794 111 64 ef ef NNP 10_1101-2021_01_07_425794 111 65 S S NNP 10_1101-2021_01_07_425794 111 66 eq eq NNP 10_1101-2021_01_07_425794 111 67 -N -n NN 10_1101-2021_01_07_425794 111 68 C C NNP 10_1101-2021_01_07_425794 111 69 B B NNP 10_1101-2021_01_07_425794 111 70 I i NN 10_1101-2021_01_07_425794 111 71 E e NN 10_1101-2021_01_07_425794 111 72 ns ns IN 10_1101-2021_01_07_425794 111 73 em -PRON- PRP 10_1101-2021_01_07_425794 111 74 bl bl PRP 10_1101-2021_01_07_425794 111 75 vs vs IN 10_1101-2021_01_07_425794 111 76 R R NNP 10_1101-2021_01_07_425794 111 77 ef ef NNP 10_1101-2021_01_07_425794 111 78 S S NNP 10_1101-2021_01_07_425794 111 79 eq eq NNP 10_1101-2021_01_07_425794 111 80 -N -n NN 10_1101-2021_01_07_425794 111 81 C C NNP 10_1101-2021_01_07_425794 111 82 B B NNP 10_1101-2021_01_07_425794 111 83 I i NN 10_1101-2021_01_07_425794 111 84 E e NN 10_1101-2021_01_07_425794 111 85 ns ns IN 10_1101-2021_01_07_425794 111 86 em -PRON- PRP 10_1101-2021_01_07_425794 111 87 bl bl PRP 10_1101-2021_01_07_425794 111 88 vs vs IN 10_1101-2021_01_07_425794 111 89 R R NNP 10_1101-2021_01_07_425794 111 90 ef ef NNP 10_1101-2021_01_07_425794 111 91 S S NNP 10_1101-2021_01_07_425794 111 92 eq eq NNP 10_1101-2021_01_07_425794 111 93 -R -R NNP 10_1101-2021_01_07_425794 111 94 su su NNP 10_1101-2021_01_07_425794 111 95 br br VBP 10_1101-2021_01_07_425794 111 96 ea ea NNP 10_1101-2021_01_07_425794 111 97 d d NN 10_1101-2021_01_07_425794 111 98 R r NN 10_1101-2021_01_07_425794 111 99 ef ef NN 10_1101-2021_01_07_425794 111 100 S S NNP 10_1101-2021_01_07_425794 111 101 eq eq NNP 10_1101-2021_01_07_425794 111 102 -N -n NN 10_1101-2021_01_07_425794 111 103 C C NNP 10_1101-2021_01_07_425794 111 104 B B NNP 10_1101-2021_01_07_425794 111 105 I I NNP 10_1101-2021_01_07_425794 111 106 vs vs IN 10_1101-2021_01_07_425794 111 107 R r NN 10_1101-2021_01_07_425794 111 108 ef ef NNP 10_1101-2021_01_07_425794 111 109 S S NNP 10_1101-2021_01_07_425794 111 110 eq eq NNP 10_1101-2021_01_07_425794 111 111 -R -R NNP 10_1101-2021_01_07_425794 111 112 su su NNP 10_1101-2021_01_07_425794 111 113 br br VBP 10_1101-2021_01_07_425794 111 114 ea ea NNP 10_1101-2021_01_07_425794 111 115 d d NN 10_1101-2021_01_07_425794 111 116 D d JJ 10_1101-2021_01_07_425794 111 117 iff iff NN 10_1101-2021_01_07_425794 111 118 er er UH 10_1101-2021_01_07_425794 111 119 en en FW 10_1101-2021_01_07_425794 111 120 ce ce NNP 10_1101-2021_01_07_425794 111 121 in in IN 10_1101-2021_01_07_425794 111 122 lo lo NNP 10_1101-2021_01_07_425794 111 123 g2 g2 NNP 10_1101-2021_01_07_425794 111 124 ef ef NNP 10_1101-2021_01_07_425794 111 125 fe fe NNP 10_1101-2021_01_07_425794 111 126 ct ct NNP 10_1101-2021_01_07_425794 111 127 iv iv NNP 10_1101-2021_01_07_425794 111 128 e e NNP 10_1101-2021_01_07_425794 111 129 ge ge NNP 10_1101-2021_01_07_425794 111 130 ne ne NNP 10_1101-2021_01_07_425794 111 131 le le NNP 10_1101-2021_01_07_425794 111 132 ng ng PRP 10_1101-2021_01_07_425794 111 133 th th XX 10_1101-2021_01_07_425794 111 134 C C NNP 10_1101-2021_01_07_425794 111 135 To to TO 10_1101-2021_01_07_425794 111 136 ta ta NNP 10_1101-2021_01_07_425794 111 137 le le NNP 10_1101-2021_01_07_425794 111 138 ffe ffe NNP 10_1101-2021_01_07_425794 111 139 ct ct NNP 10_1101-2021_01_07_425794 111 140 iv iv NNP 10_1101-2021_01_07_425794 111 141 e e NNP 10_1101-2021_01_07_425794 111 142 ge ge NNP 10_1101-2021_01_07_425794 111 143 ne ne NNP 10_1101-2021_01_07_425794 111 144 le le NNP 10_1101-2021_01_07_425794 111 145 ng ng NNP 10_1101-2021_01_07_425794 111 146 th th FW 10_1101-2021_01_07_425794 111 147 s s NNP 10_1101-2021_01_07_425794 111 148 ( ( -LRB- 10_1101-2021_01_07_425794 111 149 x x SYM 10_1101-2021_01_07_425794 111 150 10 10 CD 10_1101-2021_01_07_425794 111 151 ^6 ^6 NNP 10_1101-2021_01_07_425794 111 152 ) ) -RRB- 10_1101-2021_01_07_425794 111 153 0 0 NFP 10_1101-2021_01_07_425794 111 154 50 50 CD 10_1101-2021_01_07_425794 111 155 100 100 CD 10_1101-2021_01_07_425794 111 156 150 150 CD 10_1101-2021_01_07_425794 111 157 E E NNS 10_1101-2021_01_07_425794 111 158 ns ns IN 10_1101-2021_01_07_425794 111 159 em -PRON- PRP 10_1101-2021_01_07_425794 111 160 bl bl NNP 10_1101-2021_01_07_425794 111 161 R R NNP 10_1101-2021_01_07_425794 111 162 ef ef NNP 10_1101-2021_01_07_425794 111 163 S S NNP 10_1101-2021_01_07_425794 111 164 eq eq NNP 10_1101-2021_01_07_425794 111 165 -R -R NNP 10_1101-2021_01_07_425794 111 166 su su NNP 10_1101-2021_01_07_425794 111 167 br br VBP 10_1101-2021_01_07_425794 111 168 ea ea NNP 10_1101-2021_01_07_425794 111 169 d d NN 10_1101-2021_01_07_425794 111 170 R r NN 10_1101-2021_01_07_425794 111 171 ef ef NN 10_1101-2021_01_07_425794 111 172 S S NNP 10_1101-2021_01_07_425794 111 173 eq eq NNP 10_1101-2021_01_07_425794 111 174 -N -n NN 10_1101-2021_01_07_425794 111 175 C C NNP 10_1101-2021_01_07_425794 111 176 B B NNP 10_1101-2021_01_07_425794 111 177 I i NN 10_1101-2021_01_07_425794 111 178 D d NN 10_1101-2021_01_07_425794 111 179 3 3 CD 10_1101-2021_01_07_425794 111 180 6 6 CD 10_1101-2021_01_07_425794 111 181 9 9 CD 10_1101-2021_01_07_425794 111 182 12 12 CD 10_1101-2021_01_07_425794 111 183 15 15 CD 10_1101-2021_01_07_425794 111 184 18 18 CD 10_1101-2021_01_07_425794 111 185 −4 −4 CD 10_1101-2021_01_07_425794 111 186 −2 −2 NNP 10_1101-2021_01_07_425794 111 187 0 0 CD 10_1101-2021_01_07_425794 111 188 2 2 CD 10_1101-2021_01_07_425794 111 189 4 4 CD 10_1101-2021_01_07_425794 111 190 6 6 CD 10_1101-2021_01_07_425794 111 191 Figure figure NN 10_1101-2021_01_07_425794 111 192 1 1 CD 10_1101-2021_01_07_425794 111 193 : : : 10_1101-2021_01_07_425794 111 194 Concordance concordance NN 10_1101-2021_01_07_425794 111 195 and and CC 10_1101-2021_01_07_425794 111 196 differences difference NNS 10_1101-2021_01_07_425794 111 197 between between IN 10_1101-2021_01_07_425794 111 198 gene gene NN 10_1101-2021_01_07_425794 111 199 annotations annotation NNS 10_1101-2021_01_07_425794 111 200 . . . 10_1101-2021_01_07_425794 112 1 ( ( -LRB- 10_1101-2021_01_07_425794 112 2 A a DT 10_1101-2021_01_07_425794 112 3 ) ) -RRB- 10_1101-2021_01_07_425794 112 4 Venn Venn NNP 10_1101-2021_01_07_425794 112 5 diagram diagram NN 10_1101-2021_01_07_425794 112 6 showing show VBG 10_1101-2021_01_07_425794 112 7 genes gene NNS 10_1101-2021_01_07_425794 112 8 that that WDT 10_1101-2021_01_07_425794 112 9 are be VBP 10_1101-2021_01_07_425794 112 10 common common JJ 10_1101-2021_01_07_425794 112 11 or or CC 10_1101-2021_01_07_425794 112 12 unique unique JJ 10_1101-2021_01_07_425794 112 13 in in IN 10_1101-2021_01_07_425794 112 14 the the DT 10_1101-2021_01_07_425794 112 15 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 112 16 , , , 10_1101-2021_01_07_425794 112 17 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 112 18 - - HYPH 10_1101-2021_01_07_425794 112 19 NCBI NCBI NNP 10_1101-2021_01_07_425794 112 20 and and CC 10_1101-2021_01_07_425794 112 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 112 22 - - HYPH 10_1101-2021_01_07_425794 112 23 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 112 24 annotations annotation NNS 10_1101-2021_01_07_425794 112 25 . . . 10_1101-2021_01_07_425794 113 1 ( ( -LRB- 10_1101-2021_01_07_425794 113 2 B b NN 10_1101-2021_01_07_425794 113 3 ) ) -RRB- 10_1101-2021_01_07_425794 113 4 Boxplots boxplot NNS 10_1101-2021_01_07_425794 113 5 showing show VBG 10_1101-2021_01_07_425794 113 6 the the DT 10_1101-2021_01_07_425794 113 7 distribution distribution NN 10_1101-2021_01_07_425794 113 8 of of IN 10_1101-2021_01_07_425794 113 9 effective effective JJ 10_1101-2021_01_07_425794 113 10 gene gene NN 10_1101-2021_01_07_425794 113 11 lengths length NNS 10_1101-2021_01_07_425794 113 12 ( ( -LRB- 10_1101-2021_01_07_425794 113 13 log2 log2 NN 10_1101-2021_01_07_425794 113 14 scale scale NN 10_1101-2021_01_07_425794 113 15 ) ) -RRB- 10_1101-2021_01_07_425794 113 16 in in IN 10_1101-2021_01_07_425794 113 17 each each DT 10_1101-2021_01_07_425794 113 18 annotation annotation NN 10_1101-2021_01_07_425794 113 19 . . . 10_1101-2021_01_07_425794 114 1 ( ( -LRB- 10_1101-2021_01_07_425794 114 2 C c NN 10_1101-2021_01_07_425794 114 3 ) ) -RRB- 10_1101-2021_01_07_425794 114 4 Boxplots boxplot NNS 10_1101-2021_01_07_425794 114 5 showing show VBG 10_1101-2021_01_07_425794 114 6 the the DT 10_1101-2021_01_07_425794 114 7 differences difference NNS 10_1101-2021_01_07_425794 114 8 in in IN 10_1101-2021_01_07_425794 114 9 effective effective JJ 10_1101-2021_01_07_425794 114 10 lengths length NNS 10_1101-2021_01_07_425794 114 11 of of IN 10_1101-2021_01_07_425794 114 12 common common JJ 10_1101-2021_01_07_425794 114 13 genes gene NNS 10_1101-2021_01_07_425794 114 14 between between IN 10_1101-2021_01_07_425794 114 15 each each DT 10_1101-2021_01_07_425794 114 16 pair pair NN 10_1101-2021_01_07_425794 114 17 of of IN 10_1101-2021_01_07_425794 114 18 annotations annotation NNS 10_1101-2021_01_07_425794 114 19 . . . 10_1101-2021_01_07_425794 115 1 Values value NNS 10_1101-2021_01_07_425794 115 2 shown show VBN 10_1101-2021_01_07_425794 115 3 in in IN 10_1101-2021_01_07_425794 115 4 the the DT 10_1101-2021_01_07_425794 115 5 plots plot NNS 10_1101-2021_01_07_425794 115 6 are be VBP 10_1101-2021_01_07_425794 115 7 the the DT 10_1101-2021_01_07_425794 115 8 ratio ratio NN 10_1101-2021_01_07_425794 115 9 of of IN 10_1101-2021_01_07_425794 115 10 effective effective JJ 10_1101-2021_01_07_425794 115 11 lengths length NNS 10_1101-2021_01_07_425794 115 12 of of IN 10_1101-2021_01_07_425794 115 13 the the DT 10_1101-2021_01_07_425794 115 14 same same JJ 10_1101-2021_01_07_425794 115 15 gene gene NN 10_1101-2021_01_07_425794 115 16 from from IN 10_1101-2021_01_07_425794 115 17 two two CD 10_1101-2021_01_07_425794 115 18 different different JJ 10_1101-2021_01_07_425794 115 19 annotations annotation NNS 10_1101-2021_01_07_425794 115 20 ( ( -LRB- 10_1101-2021_01_07_425794 115 21 log2 log2 NNP 10_1101-2021_01_07_425794 115 22 scale scale NN 10_1101-2021_01_07_425794 115 23 ) ) -RRB- 10_1101-2021_01_07_425794 115 24 . . . 10_1101-2021_01_07_425794 116 1 ( ( -LRB- 10_1101-2021_01_07_425794 116 2 D d NN 10_1101-2021_01_07_425794 116 3 ) ) -RRB- 10_1101-2021_01_07_425794 116 4 The the DT 10_1101-2021_01_07_425794 116 5 size size NN 10_1101-2021_01_07_425794 116 6 of of IN 10_1101-2021_01_07_425794 116 7 transcriptome transcriptome DT 10_1101-2021_01_07_425794 116 8 calculated calculate VBN 10_1101-2021_01_07_425794 116 9 from from IN 10_1101-2021_01_07_425794 116 10 each each DT 10_1101-2021_01_07_425794 116 11 annotation annotation NN 10_1101-2021_01_07_425794 116 12 . . . 10_1101-2021_01_07_425794 117 1 Shown show VBN 10_1101-2021_01_07_425794 117 2 are be VBP 10_1101-2021_01_07_425794 117 3 the the DT 10_1101-2021_01_07_425794 117 4 sum sum NN 10_1101-2021_01_07_425794 117 5 of of IN 10_1101-2021_01_07_425794 117 6 effective effective JJ 10_1101-2021_01_07_425794 117 7 gene gene NN 10_1101-2021_01_07_425794 117 8 lengths length NNS 10_1101-2021_01_07_425794 117 9 in in IN 10_1101-2021_01_07_425794 117 10 each each DT 10_1101-2021_01_07_425794 117 11 annotation annotation NN 10_1101-2021_01_07_425794 117 12 . . . 10_1101-2021_01_07_425794 118 1 are be VBP 10_1101-2021_01_07_425794 118 2 ∼3,000 ∼3,000 CD 10_1101-2021_01_07_425794 118 3 bases basis NNS 10_1101-2021_01_07_425794 118 4 , , , 10_1101-2021_01_07_425794 118 5 which which WDT 10_1101-2021_01_07_425794 118 6 is be VBZ 10_1101-2021_01_07_425794 118 7 much much RB 10_1101-2021_01_07_425794 118 8 larger large JJR 10_1101-2021_01_07_425794 118 9 than than IN 10_1101-2021_01_07_425794 118 10 that that DT 10_1101-2021_01_07_425794 118 11 in in IN 10_1101-2021_01_07_425794 118 12 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 118 13 ( ( -LRB- 10_1101-2021_01_07_425794 118 14 ∼1,000 ∼1,000 CD 10_1101-2021_01_07_425794 118 15 bases basis NNS 10_1101-2021_01_07_425794 118 16 ) ) -RRB- 10_1101-2021_01_07_425794 118 17 . . . 10_1101-2021_01_07_425794 119 1 Although although IN 10_1101-2021_01_07_425794 119 2 the the DT 10_1101-2021_01_07_425794 119 3 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 119 4 annotation annotation NN 10_1101-2021_01_07_425794 119 5 contains contain VBZ 10_1101-2021_01_07_425794 119 6 a a DT 10_1101-2021_01_07_425794 119 7 lot lot NN 10_1101-2021_01_07_425794 119 8 more more JJR 10_1101-2021_01_07_425794 119 9 genes gene NNS 10_1101-2021_01_07_425794 119 10 than than IN 10_1101-2021_01_07_425794 119 11 the the DT 10_1101-2021_01_07_425794 119 12 two two CD 10_1101-2021_01_07_425794 119 13 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 119 14 annotations annotation NNS 10_1101-2021_01_07_425794 119 15 , , , 10_1101-2021_01_07_425794 119 16 it -PRON- PRP 10_1101-2021_01_07_425794 119 17 also also RB 10_1101-2021_01_07_425794 119 18 contains contain VBZ 10_1101-2021_01_07_425794 119 19 a a DT 10_1101-2021_01_07_425794 119 20 much much RB 10_1101-2021_01_07_425794 119 21 higher high JJR 10_1101-2021_01_07_425794 119 22 percentage percentage NN 10_1101-2021_01_07_425794 119 23 of of IN 10_1101-2021_01_07_425794 119 24 short short JJ 10_1101-2021_01_07_425794 119 25 genes gene NNS 10_1101-2021_01_07_425794 119 26 . . . 10_1101-2021_01_07_425794 120 1 We -PRON- PRP 10_1101-2021_01_07_425794 120 2 further further RB 10_1101-2021_01_07_425794 120 3 performed perform VBD 10_1101-2021_01_07_425794 120 4 gene gene NN 10_1101-2021_01_07_425794 120 5 - - HYPH 10_1101-2021_01_07_425794 120 6 wise wise JJ 10_1101-2021_01_07_425794 120 7 comparison comparison NN 10_1101-2021_01_07_425794 120 8 of of IN 10_1101-2021_01_07_425794 120 9 effective effective JJ 10_1101-2021_01_07_425794 120 10 gene gene NN 10_1101-2021_01_07_425794 120 11 lengths length NNS 10_1101-2021_01_07_425794 120 12 using use VBG 10_1101-2021_01_07_425794 120 13 common common JJ 10_1101-2021_01_07_425794 120 14 genes gene NNS 10_1101-2021_01_07_425794 120 15 between between IN 10_1101-2021_01_07_425794 120 16 each each DT 10_1101-2021_01_07_425794 120 17 pair pair NN 10_1101-2021_01_07_425794 120 18 of of IN 10_1101-2021_01_07_425794 120 19 annotations annotation NNS 10_1101-2021_01_07_425794 120 20 . . . 10_1101-2021_01_07_425794 121 1 Although although IN 10_1101-2021_01_07_425794 121 2 every every DT 10_1101-2021_01_07_425794 121 3 annotation annotation NN 10_1101-2021_01_07_425794 121 4 contains contain VBZ 10_1101-2021_01_07_425794 121 5 both both CC 10_1101-2021_01_07_425794 121 6 longer long JJR 10_1101-2021_01_07_425794 121 7 and and CC 10_1101-2021_01_07_425794 121 8 shorter short JJR 10_1101-2021_01_07_425794 121 9 genes gene NNS 10_1101-2021_01_07_425794 121 10 in in IN 10_1101-2021_01_07_425794 121 11 comparison comparison NN 10_1101-2021_01_07_425794 121 12 to to IN 10_1101-2021_01_07_425794 121 13 the the DT 10_1101-2021_01_07_425794 121 14 corresponding correspond VBG 10_1101-2021_01_07_425794 121 15 genes gene NNS 10_1101-2021_01_07_425794 121 16 from from IN 10_1101-2021_01_07_425794 121 17 other other JJ 10_1101-2021_01_07_425794 121 18 annotations annotation NNS 10_1101-2021_01_07_425794 121 19 , , , 10_1101-2021_01_07_425794 121 20 the the DT 10_1101-2021_01_07_425794 121 21 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 121 22 genes gene NNS 10_1101-2021_01_07_425794 121 23 were be VBD 10_1101-2021_01_07_425794 121 24 found find VBN 10_1101-2021_01_07_425794 121 25 to to TO 10_1101-2021_01_07_425794 121 26 have have VB 10_1101-2021_01_07_425794 121 27 a a DT 10_1101-2021_01_07_425794 121 28 larger large JJR 10_1101-2021_01_07_425794 121 29 effective effective JJ 10_1101-2021_01_07_425794 121 30 length length NN 10_1101-2021_01_07_425794 121 31 than than IN 10_1101-2021_01_07_425794 121 32 genes gene NNS 10_1101-2021_01_07_425794 121 33 from from IN 10_1101-2021_01_07_425794 121 34 the the DT 10_1101-2021_01_07_425794 121 35 two two CD 10_1101-2021_01_07_425794 121 36 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 121 37 annotations annotation NNS 10_1101-2021_01_07_425794 121 38 overall overall JJ 10_1101-2021_01_07_425794 121 39 ( ( -LRB- 10_1101-2021_01_07_425794 121 40 Figure figure NN 10_1101-2021_01_07_425794 121 41 1C 1c NN 10_1101-2021_01_07_425794 121 42 ) ) -RRB- 10_1101-2021_01_07_425794 121 43 . . . 10_1101-2021_01_07_425794 122 1 This this DT 10_1101-2021_01_07_425794 122 2 is be VBZ 10_1101-2021_01_07_425794 122 3 in in IN 10_1101-2021_01_07_425794 122 4 contrast contrast NN 10_1101-2021_01_07_425794 122 5 to to IN 10_1101-2021_01_07_425794 122 6 the the DT 10_1101-2021_01_07_425794 122 7 higher high JJR 10_1101-2021_01_07_425794 122 8 proportion proportion NN 10_1101-2021_01_07_425794 122 9 of of IN 10_1101-2021_01_07_425794 122 10 short short JJ 10_1101-2021_01_07_425794 122 11 genes gene NNS 10_1101-2021_01_07_425794 122 12 observed observe VBN 10_1101-2021_01_07_425794 122 13 in in IN 10_1101-2021_01_07_425794 122 14 the the DT 10_1101-2021_01_07_425794 122 15 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 122 16 annotation annotation NN 10_1101-2021_01_07_425794 122 17 ( ( -LRB- 10_1101-2021_01_07_425794 122 18 Figure figure NN 10_1101-2021_01_07_425794 122 19 1B 1b NN 10_1101-2021_01_07_425794 122 20 ) ) -RRB- 10_1101-2021_01_07_425794 122 21 , , , 10_1101-2021_01_07_425794 122 22 which which WDT 10_1101-2021_01_07_425794 122 23 indicates indicate VBZ 10_1101-2021_01_07_425794 122 24 that that IN 10_1101-2021_01_07_425794 122 25 the the DT 10_1101-2021_01_07_425794 122 26 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 122 27 genes gene NNS 10_1101-2021_01_07_425794 122 28 that that WDT 10_1101-2021_01_07_425794 122 29 are be VBP 10_1101-2021_01_07_425794 122 30 also also RB 10_1101-2021_01_07_425794 122 31 present present JJ 10_1101-2021_01_07_425794 122 32 in in IN 10_1101-2021_01_07_425794 122 33 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 122 34 - - HYPH 10_1101-2021_01_07_425794 122 35 NCBI NCBI NNP 10_1101-2021_01_07_425794 122 36 or or CC 10_1101-2021_01_07_425794 122 37 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 122 38 - - HYPH 10_1101-2021_01_07_425794 122 39 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 122 40 annotations annotation NNS 10_1101-2021_01_07_425794 122 41 tend tend VBP 10_1101-2021_01_07_425794 122 42 to to TO 10_1101-2021_01_07_425794 122 43 be be VB 10_1101-2021_01_07_425794 122 44 longer long JJR 10_1101-2021_01_07_425794 122 45 than than IN 10_1101-2021_01_07_425794 122 46 those those DT 10_1101-2021_01_07_425794 122 47 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 122 48 genes gene NNS 10_1101-2021_01_07_425794 122 49 that that WDT 10_1101-2021_01_07_425794 122 50 can can MD 10_1101-2021_01_07_425794 122 51 only only RB 10_1101-2021_01_07_425794 122 52 be be VB 10_1101-2021_01_07_425794 122 53 found find VBN 10_1101-2021_01_07_425794 122 54 in in IN 10_1101-2021_01_07_425794 122 55 the the DT 10_1101-2021_01_07_425794 122 56 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 122 57 annotation annotation NN 10_1101-2021_01_07_425794 122 58 . . . 10_1101-2021_01_07_425794 123 1 Although although IN 10_1101-2021_01_07_425794 123 2 at at RB 10_1101-2021_01_07_425794 123 3 least least JJS 10_1101-2021_01_07_425794 123 4 half half NN 10_1101-2021_01_07_425794 123 5 of of IN 10_1101-2021_01_07_425794 123 6 the the DT 10_1101-2021_01_07_425794 123 7 genes gene NNS 10_1101-2021_01_07_425794 123 8 were be VBD 10_1101-2021_01_07_425794 123 9 found find VBN 10_1101-2021_01_07_425794 123 10 to to TO 10_1101-2021_01_07_425794 123 11 have have VB 10_1101-2021_01_07_425794 123 12 a a DT 10_1101-2021_01_07_425794 123 13 less less JJR 10_1101-2021_01_07_425794 123 14 than than IN 10_1101-2021_01_07_425794 123 15 2-fold 2-fold CD 10_1101-2021_01_07_425794 123 16 ( ( -LRB- 10_1101-2021_01_07_425794 123 17 1-fold 1-fold CD 10_1101-2021_01_07_425794 123 18 at at IN 10_1101-2021_01_07_425794 123 19 log2 log2 NN 10_1101-2021_01_07_425794 123 20 scale scale NN 10_1101-2021_01_07_425794 123 21 ) ) -RRB- 10_1101-2021_01_07_425794 123 22 length length NN 10_1101-2021_01_07_425794 123 23 difference difference NN 10_1101-2021_01_07_425794 123 24 between between IN 10_1101-2021_01_07_425794 123 25 annotations annotation NNS 10_1101-2021_01_07_425794 123 26 ( ( -LRB- 10_1101-2021_01_07_425794 123 27 Figure figure NN 10_1101-2021_01_07_425794 123 28 1C 1c NN 10_1101-2021_01_07_425794 123 29 ) ) -RRB- 10_1101-2021_01_07_425794 123 30 , , , 10_1101-2021_01_07_425794 123 31 the the DT 10_1101-2021_01_07_425794 123 32 length length NN 10_1101-2021_01_07_425794 123 33 differences difference NNS 10_1101-2021_01_07_425794 123 34 could could MD 10_1101-2021_01_07_425794 123 35 be be VB 10_1101-2021_01_07_425794 123 36 as as RB 10_1101-2021_01_07_425794 123 37 high high JJ 10_1101-2021_01_07_425794 123 38 as as IN 10_1101-2021_01_07_425794 123 39 more more JJR 10_1101-2021_01_07_425794 123 40 than than IN 10_1101-2021_01_07_425794 123 41 64-folds 64-folds CD 10_1101-2021_01_07_425794 123 42 ( ( -LRB- 10_1101-2021_01_07_425794 123 43 6-folds 6-folds CD 10_1101-2021_01_07_425794 123 44 at at IN 10_1101-2021_01_07_425794 123 45 log2 log2 NN 10_1101-2021_01_07_425794 123 46 scale scale NN 10_1101-2021_01_07_425794 123 47 ) ) -RRB- 10_1101-2021_01_07_425794 123 48 . . . 10_1101-2021_01_07_425794 124 1 The the DT 10_1101-2021_01_07_425794 124 2 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 124 3 - - HYPH 10_1101-2021_01_07_425794 124 4 NCBI NCBI NNP 10_1101-2021_01_07_425794 124 5 genes gene NNS 10_1101-2021_01_07_425794 124 6 seem seem VBP 10_1101-2021_01_07_425794 124 7 to to IN 10_1101-2021_01_07_425794 124 8 8 8 CD 10_1101-2021_01_07_425794 124 9 .CC .CC NFP 10_1101-2021_01_07_425794 124 10 - - HYPH 10_1101-2021_01_07_425794 124 11 BY by IN 10_1101-2021_01_07_425794 124 12 - - HYPH 10_1101-2021_01_07_425794 124 13 NC NC NNP 10_1101-2021_01_07_425794 124 14 - - HYPH 10_1101-2021_01_07_425794 124 15 ND ND NNP 10_1101-2021_01_07_425794 124 16 4.0 4.0 CD 10_1101-2021_01_07_425794 124 17 International international JJ 10_1101-2021_01_07_425794 124 18 licensemade licensemade NN 10_1101-2021_01_07_425794 124 19 available available JJ 10_1101-2021_01_07_425794 124 20 under under IN 10_1101-2021_01_07_425794 124 21 a a DT 10_1101-2021_01_07_425794 124 22 ( ( -LRB- 10_1101-2021_01_07_425794 124 23 which which WDT 10_1101-2021_01_07_425794 124 24 was be VBD 10_1101-2021_01_07_425794 124 25 not not RB 10_1101-2021_01_07_425794 124 26 certified certify VBN 10_1101-2021_01_07_425794 124 27 by by IN 10_1101-2021_01_07_425794 124 28 peer peer NN 10_1101-2021_01_07_425794 124 29 review review NN 10_1101-2021_01_07_425794 124 30 ) ) -RRB- 10_1101-2021_01_07_425794 124 31 is be VBZ 10_1101-2021_01_07_425794 124 32 the the DT 10_1101-2021_01_07_425794 124 33 author author NN 10_1101-2021_01_07_425794 124 34 / / SYM 10_1101-2021_01_07_425794 124 35 funder funder NN 10_1101-2021_01_07_425794 124 36 , , , 10_1101-2021_01_07_425794 124 37 who who WP 10_1101-2021_01_07_425794 124 38 has have VBZ 10_1101-2021_01_07_425794 124 39 granted grant VBN 10_1101-2021_01_07_425794 124 40 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 124 41 a a DT 10_1101-2021_01_07_425794 124 42 license license NN 10_1101-2021_01_07_425794 124 43 to to TO 10_1101-2021_01_07_425794 124 44 display display VB 10_1101-2021_01_07_425794 124 45 the the DT 10_1101-2021_01_07_425794 124 46 preprint preprint NN 10_1101-2021_01_07_425794 124 47 in in IN 10_1101-2021_01_07_425794 124 48 perpetuity perpetuity NN 10_1101-2021_01_07_425794 124 49 . . . 10_1101-2021_01_07_425794 125 1 It -PRON- PRP 10_1101-2021_01_07_425794 125 2 is be VBZ 10_1101-2021_01_07_425794 125 3 The the DT 10_1101-2021_01_07_425794 125 4 copyright copyright NN 10_1101-2021_01_07_425794 125 5 holder holder NN 10_1101-2021_01_07_425794 125 6 for for IN 10_1101-2021_01_07_425794 125 7 this this DT 10_1101-2021_01_07_425794 125 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 125 9 version version NN 10_1101-2021_01_07_425794 125 10 posted post VBD 10_1101-2021_01_07_425794 125 11 January January NNP 10_1101-2021_01_07_425794 125 12 8 8 CD 10_1101-2021_01_07_425794 125 13 , , , 10_1101-2021_01_07_425794 125 14 2021 2021 CD 10_1101-2021_01_07_425794 125 15 . . . 10_1101-2021_01_07_425794 125 16 ; ; : 10_1101-2021_01_07_425794 125 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 125 18 : : : 10_1101-2021_01_07_425794 125 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 125 20 preprint preprint JJ 10_1101-2021_01_07_425794 125 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 125 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 125 23 be be VB 10_1101-2021_01_07_425794 125 24 slightly slightly RB 10_1101-2021_01_07_425794 125 25 longer long JJR 10_1101-2021_01_07_425794 125 26 than than IN 10_1101-2021_01_07_425794 125 27 the the DT 10_1101-2021_01_07_425794 125 28 corresponding corresponding JJ 10_1101-2021_01_07_425794 125 29 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 125 30 - - HYPH 10_1101-2021_01_07_425794 125 31 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 125 32 genes gene NNS 10_1101-2021_01_07_425794 125 33 overall overall RB 10_1101-2021_01_07_425794 125 34 . . . 10_1101-2021_01_07_425794 126 1 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 126 2 and and CC 10_1101-2021_01_07_425794 126 3 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 126 4 - - HYPH 10_1101-2021_01_07_425794 126 5 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 126 6 were be VBD 10_1101-2021_01_07_425794 126 7 found find VBN 10_1101-2021_01_07_425794 126 8 to to TO 10_1101-2021_01_07_425794 126 9 be be VB 10_1101-2021_01_07_425794 126 10 the the DT 10_1101-2021_01_07_425794 126 11 least least JJS 10_1101-2021_01_07_425794 126 12 concordant concordant NN 10_1101-2021_01_07_425794 126 13 annotations annotation NNS 10_1101-2021_01_07_425794 126 14 among among IN 10_1101-2021_01_07_425794 126 15 the the DT 10_1101-2021_01_07_425794 126 16 three three CD 10_1101-2021_01_07_425794 126 17 annotations annotation NNS 10_1101-2021_01_07_425794 126 18 being be VBG 10_1101-2021_01_07_425794 126 19 compared compare VBN 10_1101-2021_01_07_425794 126 20 . . . 10_1101-2021_01_07_425794 127 1 Lastly lastly RB 10_1101-2021_01_07_425794 127 2 , , , 10_1101-2021_01_07_425794 127 3 we -PRON- PRP 10_1101-2021_01_07_425794 127 4 compared compare VBD 10_1101-2021_01_07_425794 127 5 the the DT 10_1101-2021_01_07_425794 127 6 size size NN 10_1101-2021_01_07_425794 127 7 of of IN 10_1101-2021_01_07_425794 127 8 the the DT 10_1101-2021_01_07_425794 127 9 transcriptome transcriptome DT 10_1101-2021_01_07_425794 127 10 represented represent VBN 10_1101-2021_01_07_425794 127 11 by by IN 10_1101-2021_01_07_425794 127 12 each each DT 10_1101-2021_01_07_425794 127 13 annotation annotation NN 10_1101-2021_01_07_425794 127 14 . . . 10_1101-2021_01_07_425794 128 1 The the DT 10_1101-2021_01_07_425794 128 2 transcriptome transcriptome DT 10_1101-2021_01_07_425794 128 3 size size NN 10_1101-2021_01_07_425794 128 4 of of IN 10_1101-2021_01_07_425794 128 5 an an DT 10_1101-2021_01_07_425794 128 6 annotation annotation NN 10_1101-2021_01_07_425794 128 7 is be VBZ 10_1101-2021_01_07_425794 128 8 computed compute VBN 10_1101-2021_01_07_425794 128 9 as as IN 10_1101-2021_01_07_425794 128 10 the the DT 10_1101-2021_01_07_425794 128 11 sum sum NN 10_1101-2021_01_07_425794 128 12 of of IN 10_1101-2021_01_07_425794 128 13 effective effective JJ 10_1101-2021_01_07_425794 128 14 gene gene NN 10_1101-2021_01_07_425794 128 15 lengths length NNS 10_1101-2021_01_07_425794 128 16 from from IN 10_1101-2021_01_07_425794 128 17 all all PDT 10_1101-2021_01_07_425794 128 18 the the DT 10_1101-2021_01_07_425794 128 19 genes gene NNS 10_1101-2021_01_07_425794 128 20 included include VBN 10_1101-2021_01_07_425794 128 21 in in IN 10_1101-2021_01_07_425794 128 22 that that DT 10_1101-2021_01_07_425794 128 23 annotation annotation NN 10_1101-2021_01_07_425794 128 24 , , , 10_1101-2021_01_07_425794 128 25 which which WDT 10_1101-2021_01_07_425794 128 26 also also RB 10_1101-2021_01_07_425794 128 27 represents represent VBZ 10_1101-2021_01_07_425794 128 28 the the DT 10_1101-2021_01_07_425794 128 29 total total JJ 10_1101-2021_01_07_425794 128 30 num- num- NNP 10_1101-2021_01_07_425794 128 31 ber ber NN 10_1101-2021_01_07_425794 128 32 of of IN 10_1101-2021_01_07_425794 128 33 exonic exonic JJ 10_1101-2021_01_07_425794 128 34 bases basis NNS 10_1101-2021_01_07_425794 128 35 that that WDT 10_1101-2021_01_07_425794 128 36 were be VBD 10_1101-2021_01_07_425794 128 37 annotated annotate VBN 10_1101-2021_01_07_425794 128 38 in in IN 10_1101-2021_01_07_425794 128 39 an an DT 10_1101-2021_01_07_425794 128 40 annotation annotation NN 10_1101-2021_01_07_425794 128 41 . . . 10_1101-2021_01_07_425794 129 1 Figure figure NN 10_1101-2021_01_07_425794 129 2 1D 1D NNP 10_1101-2021_01_07_425794 129 3 shows show VBZ 10_1101-2021_01_07_425794 129 4 that that IN 10_1101-2021_01_07_425794 129 5 the the DT 10_1101-2021_01_07_425794 129 6 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 129 7 annotation annotation NN 10_1101-2021_01_07_425794 129 8 has have VBZ 10_1101-2021_01_07_425794 129 9 a a DT 10_1101-2021_01_07_425794 129 10 larger large JJR 10_1101-2021_01_07_425794 129 11 transcriptome transcriptome DT 10_1101-2021_01_07_425794 129 12 size size NN 10_1101-2021_01_07_425794 129 13 than than IN 10_1101-2021_01_07_425794 129 14 both both DT 10_1101-2021_01_07_425794 129 15 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 129 16 - - HYPH 10_1101-2021_01_07_425794 129 17 NCBI NCBI NNP 10_1101-2021_01_07_425794 129 18 and and CC 10_1101-2021_01_07_425794 129 19 RefSeq- RefSeq- NNP 10_1101-2021_01_07_425794 129 20 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 129 21 annotations annotation NNS 10_1101-2021_01_07_425794 129 22 . . . 10_1101-2021_01_07_425794 130 1 This this DT 10_1101-2021_01_07_425794 130 2 is be VBZ 10_1101-2021_01_07_425794 130 3 not not RB 10_1101-2021_01_07_425794 130 4 surprising surprising JJ 10_1101-2021_01_07_425794 130 5 because because IN 10_1101-2021_01_07_425794 130 6 the the DT 10_1101-2021_01_07_425794 130 7 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 130 8 annotation annotation NN 10_1101-2021_01_07_425794 130 9 contains contain VBZ 10_1101-2021_01_07_425794 130 10 more more JJR 10_1101-2021_01_07_425794 130 11 genes gene NNS 10_1101-2021_01_07_425794 130 12 and and CC 10_1101-2021_01_07_425794 130 13 also also RB 10_1101-2021_01_07_425794 130 14 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 130 15 genes gene NNS 10_1101-2021_01_07_425794 130 16 common common JJ 10_1101-2021_01_07_425794 130 17 to to IN 10_1101-2021_01_07_425794 130 18 other other JJ 10_1101-2021_01_07_425794 130 19 annotations annotation NNS 10_1101-2021_01_07_425794 130 20 are be VBP 10_1101-2021_01_07_425794 130 21 longer long JJR 10_1101-2021_01_07_425794 130 22 in in IN 10_1101-2021_01_07_425794 130 23 general general JJ 10_1101-2021_01_07_425794 130 24 . . . 10_1101-2021_01_07_425794 131 1 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 131 2 - - HYPH 10_1101-2021_01_07_425794 131 3 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 131 4 has have VBZ 10_1101-2021_01_07_425794 131 5 a a DT 10_1101-2021_01_07_425794 131 6 much much RB 10_1101-2021_01_07_425794 131 7 smaller small JJR 10_1101-2021_01_07_425794 131 8 transcriptome transcriptome DT 10_1101-2021_01_07_425794 131 9 size size NN 10_1101-2021_01_07_425794 131 10 than than IN 10_1101-2021_01_07_425794 131 11 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 131 12 - - HYPH 10_1101-2021_01_07_425794 131 13 NCBI NCBI NNP 10_1101-2021_01_07_425794 131 14 , , , 10_1101-2021_01_07_425794 131 15 indicating indicate VBG 10_1101-2021_01_07_425794 131 16 a a DT 10_1101-2021_01_07_425794 131 17 significant significant JJ 10_1101-2021_01_07_425794 131 18 expansion expansion NN 10_1101-2021_01_07_425794 131 19 of of IN 10_1101-2021_01_07_425794 131 20 the the DT 10_1101-2021_01_07_425794 131 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 131 22 - - HYPH 10_1101-2021_01_07_425794 131 23 NCBI NCBI NNP 10_1101-2021_01_07_425794 131 24 annotation annotation NN 10_1101-2021_01_07_425794 131 25 in in IN 10_1101-2021_01_07_425794 131 26 the the DT 10_1101-2021_01_07_425794 131 27 past past JJ 10_1101-2021_01_07_425794 131 28 five five CD 10_1101-2021_01_07_425794 131 29 years year NNS 10_1101-2021_01_07_425794 131 30 . . . 10_1101-2021_01_07_425794 132 1 However however RB 10_1101-2021_01_07_425794 132 2 , , , 10_1101-2021_01_07_425794 132 3 it -PRON- PRP 10_1101-2021_01_07_425794 132 4 is be VBZ 10_1101-2021_01_07_425794 132 5 important important JJ 10_1101-2021_01_07_425794 132 6 to to TO 10_1101-2021_01_07_425794 132 7 note note VB 10_1101-2021_01_07_425794 132 8 that that IN 10_1101-2021_01_07_425794 132 9 the the DT 10_1101-2021_01_07_425794 132 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 132 11 - - HYPH 10_1101-2021_01_07_425794 132 12 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 132 13 annotation annotation NN 10_1101-2021_01_07_425794 132 14 is be VBZ 10_1101-2021_01_07_425794 132 15 not not RB 10_1101-2021_01_07_425794 132 16 a a DT 10_1101-2021_01_07_425794 132 17 subset subset NN 10_1101-2021_01_07_425794 132 18 of of IN 10_1101-2021_01_07_425794 132 19 the the DT 10_1101-2021_01_07_425794 132 20 RefSeq- refseq- JJ 10_1101-2021_01_07_425794 132 21 NCBI NCBI NNP 10_1101-2021_01_07_425794 132 22 annotation annotation NN 10_1101-2021_01_07_425794 132 23 , , , 10_1101-2021_01_07_425794 132 24 as as IN 10_1101-2021_01_07_425794 132 25 demonstrated demonstrate VBN 10_1101-2021_01_07_425794 132 26 by by IN 10_1101-2021_01_07_425794 132 27 the the DT 10_1101-2021_01_07_425794 132 28 existence existence NN 10_1101-2021_01_07_425794 132 29 of of IN 10_1101-2021_01_07_425794 132 30 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 132 31 - - HYPH 10_1101-2021_01_07_425794 132 32 RSubread RSubread NNP 10_1101-2021_01_07_425794 132 33 genes gene NNS 10_1101-2021_01_07_425794 132 34 that that WDT 10_1101-2021_01_07_425794 132 35 are be VBP 10_1101-2021_01_07_425794 132 36 absent absent JJ 10_1101-2021_01_07_425794 132 37 in in IN 10_1101-2021_01_07_425794 132 38 the the DT 10_1101-2021_01_07_425794 132 39 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 132 40 - - HYPH 10_1101-2021_01_07_425794 132 41 NCBI NCBI NNP 10_1101-2021_01_07_425794 132 42 annotation annotation NN 10_1101-2021_01_07_425794 132 43 , , , 10_1101-2021_01_07_425794 132 44 the the DT 10_1101-2021_01_07_425794 132 45 difference difference NN 10_1101-2021_01_07_425794 132 46 in in IN 10_1101-2021_01_07_425794 132 47 gene gene NN 10_1101-2021_01_07_425794 132 48 length length NN 10_1101-2021_01_07_425794 132 49 distribution distribution NN 10_1101-2021_01_07_425794 132 50 and and CC 10_1101-2021_01_07_425794 132 51 the the DT 10_1101-2021_01_07_425794 132 52 length length NN 10_1101-2021_01_07_425794 132 53 differences difference NNS 10_1101-2021_01_07_425794 132 54 of of IN 10_1101-2021_01_07_425794 132 55 the the DT 10_1101-2021_01_07_425794 132 56 same same JJ 10_1101-2021_01_07_425794 132 57 genes gene NNS 10_1101-2021_01_07_425794 132 58 between between IN 10_1101-2021_01_07_425794 132 59 the the DT 10_1101-2021_01_07_425794 132 60 two two CD 10_1101-2021_01_07_425794 132 61 annotations annotation NNS 10_1101-2021_01_07_425794 132 62 ( ( -LRB- 10_1101-2021_01_07_425794 132 63 Figure figure NN 10_1101-2021_01_07_425794 132 64 1A 1a CD 10_1101-2021_01_07_425794 132 65 - - HYPH 10_1101-2021_01_07_425794 132 66 C c NN 10_1101-2021_01_07_425794 132 67 ) ) -RRB- 10_1101-2021_01_07_425794 132 68 . . . 10_1101-2021_01_07_425794 133 1 This this DT 10_1101-2021_01_07_425794 133 2 indicates indicate VBZ 10_1101-2021_01_07_425794 133 3 that that IN 10_1101-2021_01_07_425794 133 4 not not RB 10_1101-2021_01_07_425794 133 5 only only RB 10_1101-2021_01_07_425794 133 6 were be VBD 10_1101-2021_01_07_425794 133 7 new new JJ 10_1101-2021_01_07_425794 133 8 genes gene NNS 10_1101-2021_01_07_425794 133 9 added add VBN 10_1101-2021_01_07_425794 133 10 to to IN 10_1101-2021_01_07_425794 133 11 the the DT 10_1101-2021_01_07_425794 133 12 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 133 13 annotation annotation NN 10_1101-2021_01_07_425794 133 14 during during IN 10_1101-2021_01_07_425794 133 15 the the DT 10_1101-2021_01_07_425794 133 16 expansion expansion NN 10_1101-2021_01_07_425794 133 17 , , , 10_1101-2021_01_07_425794 133 18 but but CC 10_1101-2021_01_07_425794 133 19 existing exist VBG 10_1101-2021_01_07_425794 133 20 genes gene NNS 10_1101-2021_01_07_425794 133 21 have have VBP 10_1101-2021_01_07_425794 133 22 been be VBN 10_1101-2021_01_07_425794 133 23 modified modify VBN 10_1101-2021_01_07_425794 133 24 . . . 10_1101-2021_01_07_425794 134 1 It -PRON- PRP 10_1101-2021_01_07_425794 134 2 is be VBZ 10_1101-2021_01_07_425794 134 3 against against IN 10_1101-2021_01_07_425794 134 4 this this DT 10_1101-2021_01_07_425794 134 5 background background NN 10_1101-2021_01_07_425794 134 6 that that WDT 10_1101-2021_01_07_425794 134 7 we -PRON- PRP 10_1101-2021_01_07_425794 134 8 sought seek VBD 10_1101-2021_01_07_425794 134 9 to to TO 10_1101-2021_01_07_425794 134 10 understand understand VB 10_1101-2021_01_07_425794 134 11 how how WRB 10_1101-2021_01_07_425794 134 12 these these DT 10_1101-2021_01_07_425794 134 13 differences difference NNS 10_1101-2021_01_07_425794 134 14 in in IN 10_1101-2021_01_07_425794 134 15 the the DT 10_1101-2021_01_07_425794 134 16 annotations annotation NNS 10_1101-2021_01_07_425794 134 17 impact impact NN 10_1101-2021_01_07_425794 134 18 on on IN 10_1101-2021_01_07_425794 134 19 the the DT 10_1101-2021_01_07_425794 134 20 overall overall JJ 10_1101-2021_01_07_425794 134 21 gene gene NN 10_1101-2021_01_07_425794 134 22 - - HYPH 10_1101-2021_01_07_425794 134 23 level level NN 10_1101-2021_01_07_425794 134 24 quantification quantification NN 10_1101-2021_01_07_425794 134 25 results result NNS 10_1101-2021_01_07_425794 134 26 . . . 10_1101-2021_01_07_425794 135 1 3.2 3.2 CD 10_1101-2021_01_07_425794 135 2 Fragments Fragments NNPS 10_1101-2021_01_07_425794 135 3 counted count VBD 10_1101-2021_01_07_425794 135 4 to to IN 10_1101-2021_01_07_425794 135 5 annotated annotate VBN 10_1101-2021_01_07_425794 135 6 genes gene NNS 10_1101-2021_01_07_425794 135 7 We -PRON- PRP 10_1101-2021_01_07_425794 135 8 used use VBD 10_1101-2021_01_07_425794 135 9 a a DT 10_1101-2021_01_07_425794 135 10 benchmark benchmark JJ 10_1101-2021_01_07_425794 135 11 RNA RNA NNP 10_1101-2021_01_07_425794 135 12 - - HYPH 10_1101-2021_01_07_425794 135 13 seq seq NN 10_1101-2021_01_07_425794 135 14 dataset dataset NN 10_1101-2021_01_07_425794 135 15 generated generate VBN 10_1101-2021_01_07_425794 135 16 by by IN 10_1101-2021_01_07_425794 135 17 the the DT 10_1101-2021_01_07_425794 135 18 SEQC SEQC NNP 10_1101-2021_01_07_425794 135 19 project project NN 10_1101-2021_01_07_425794 135 20 [ [ -LRB- 10_1101-2021_01_07_425794 135 21 1 1 CD 10_1101-2021_01_07_425794 135 22 ] ] -RRB- 10_1101-2021_01_07_425794 135 23 to to TO 10_1101-2021_01_07_425794 135 24 evaluate evaluate VB 10_1101-2021_01_07_425794 135 25 the the DT 10_1101-2021_01_07_425794 135 26 impact impact NN 10_1101-2021_01_07_425794 135 27 of of IN 10_1101-2021_01_07_425794 135 28 gene gene NN 10_1101-2021_01_07_425794 135 29 annotation annotation NN 10_1101-2021_01_07_425794 135 30 on on IN 10_1101-2021_01_07_425794 135 31 the the DT 10_1101-2021_01_07_425794 135 32 accuracy accuracy NN 10_1101-2021_01_07_425794 135 33 of of IN 10_1101-2021_01_07_425794 135 34 RNA RNA NNP 10_1101-2021_01_07_425794 135 35 - - HYPH 10_1101-2021_01_07_425794 135 36 seq seq NN 10_1101-2021_01_07_425794 135 37 expression expression NN 10_1101-2021_01_07_425794 135 38 quantification quantification NN 10_1101-2021_01_07_425794 135 39 . . . 10_1101-2021_01_07_425794 136 1 This this DT 10_1101-2021_01_07_425794 136 2 dataset dataset NN 10_1101-2021_01_07_425794 136 3 contains contain VBZ 10_1101-2021_01_07_425794 136 4 paired pair VBN 10_1101-2021_01_07_425794 136 5 - - HYPH 10_1101-2021_01_07_425794 136 6 end end NN 10_1101-2021_01_07_425794 136 7 100bp 100bp NNP 10_1101-2021_01_07_425794 136 8 read read VB 10_1101-2021_01_07_425794 136 9 data datum NNS 10_1101-2021_01_07_425794 136 10 generated generate VBN 10_1101-2021_01_07_425794 136 11 for for IN 10_1101-2021_01_07_425794 136 12 four four CD 10_1101-2021_01_07_425794 136 13 samples sample NNS 10_1101-2021_01_07_425794 136 14 including include VBG 10_1101-2021_01_07_425794 136 15 a a DT 10_1101-2021_01_07_425794 136 16 Universal Universal NNP 10_1101-2021_01_07_425794 136 17 Human Human NNP 10_1101-2021_01_07_425794 136 18 Reference Reference NNP 10_1101-2021_01_07_425794 136 19 RNA RNA NNP 10_1101-2021_01_07_425794 136 20 sample sample NN 10_1101-2021_01_07_425794 136 21 ( ( -LRB- 10_1101-2021_01_07_425794 136 22 sample sample NNP 10_1101-2021_01_07_425794 136 23 A A NNP 10_1101-2021_01_07_425794 136 24 ) ) -RRB- 10_1101-2021_01_07_425794 136 25 , , , 10_1101-2021_01_07_425794 136 26 a a DT 10_1101-2021_01_07_425794 136 27 Human Human NNP 10_1101-2021_01_07_425794 136 28 Brain Brain NNP 10_1101-2021_01_07_425794 136 29 Reference Reference NNP 10_1101-2021_01_07_425794 136 30 RNA RNA NNP 10_1101-2021_01_07_425794 136 31 sample sample NN 10_1101-2021_01_07_425794 136 32 ( ( -LRB- 10_1101-2021_01_07_425794 136 33 sample sample NNP 10_1101-2021_01_07_425794 136 34 B B NNP 10_1101-2021_01_07_425794 136 35 ) ) -RRB- 10_1101-2021_01_07_425794 136 36 , , , 10_1101-2021_01_07_425794 136 37 a a DT 10_1101-2021_01_07_425794 136 38 mixture mixture NN 10_1101-2021_01_07_425794 136 39 sample sample NN 10_1101-2021_01_07_425794 136 40 with with IN 10_1101-2021_01_07_425794 136 41 75%A 75%a CD 10_1101-2021_01_07_425794 136 42 and and CC 10_1101-2021_01_07_425794 136 43 25%B 25%b CD 10_1101-2021_01_07_425794 136 44 ( ( -LRB- 10_1101-2021_01_07_425794 136 45 sample sample NN 10_1101-2021_01_07_425794 136 46 C C NNP 10_1101-2021_01_07_425794 136 47 ) ) -RRB- 10_1101-2021_01_07_425794 136 48 and and CC 10_1101-2021_01_07_425794 136 49 a a DT 10_1101-2021_01_07_425794 136 50 mixture mixture NN 10_1101-2021_01_07_425794 136 51 sample sample NN 10_1101-2021_01_07_425794 136 52 with with IN 10_1101-2021_01_07_425794 136 53 25%A 25%a CD 10_1101-2021_01_07_425794 136 54 and and CC 10_1101-2021_01_07_425794 136 55 75%B 75%b CD 10_1101-2021_01_07_425794 136 56 ( ( -LRB- 10_1101-2021_01_07_425794 136 57 sample sample NN 10_1101-2021_01_07_425794 136 58 D D NNP 10_1101-2021_01_07_425794 136 59 ) ) -RRB- 10_1101-2021_01_07_425794 136 60 . . . 10_1101-2021_01_07_425794 137 1 We -PRON- PRP 10_1101-2021_01_07_425794 137 2 mapped map VBD 10_1101-2021_01_07_425794 137 3 the the DT 10_1101-2021_01_07_425794 137 4 RNA RNA NNP 10_1101-2021_01_07_425794 137 5 - - HYPH 10_1101-2021_01_07_425794 137 6 seq seq NN 10_1101-2021_01_07_425794 137 7 reads read VBZ 10_1101-2021_01_07_425794 137 8 to to IN 10_1101-2021_01_07_425794 137 9 the the DT 10_1101-2021_01_07_425794 137 10 human human JJ 10_1101-2021_01_07_425794 137 11 genome genome NNP 10_1101-2021_01_07_425794 137 12 GRCh38 GRCh38 NNP 10_1101-2021_01_07_425794 137 13 / / SYM 10_1101-2021_01_07_425794 137 14 hg38 hg38 NNP 10_1101-2021_01_07_425794 137 15 using use VBG 10_1101-2021_01_07_425794 137 16 the the DT 10_1101-2021_01_07_425794 137 17 Sub- Sub- NNP 10_1101-2021_01_07_425794 137 18 read read VBN 10_1101-2021_01_07_425794 137 19 aligner aligner NN 10_1101-2021_01_07_425794 137 20 [ [ -LRB- 10_1101-2021_01_07_425794 137 21 20 20 CD 10_1101-2021_01_07_425794 137 22 , , , 10_1101-2021_01_07_425794 137 23 5 5 CD 10_1101-2021_01_07_425794 137 24 ] ] -RRB- 10_1101-2021_01_07_425794 137 25 , , , 10_1101-2021_01_07_425794 137 26 and and CC 10_1101-2021_01_07_425794 137 27 then then RB 10_1101-2021_01_07_425794 137 28 counted count VBD 10_1101-2021_01_07_425794 137 29 the the DT 10_1101-2021_01_07_425794 137 30 number number NN 10_1101-2021_01_07_425794 137 31 of of IN 10_1101-2021_01_07_425794 137 32 mapped map VBN 10_1101-2021_01_07_425794 137 33 fragments fragment NNS 10_1101-2021_01_07_425794 137 34 ( ( -LRB- 10_1101-2021_01_07_425794 137 35 read read VB 10_1101-2021_01_07_425794 137 36 pairs pair NNS 10_1101-2021_01_07_425794 137 37 ) ) -RRB- 10_1101-2021_01_07_425794 137 38 to to IN 10_1101-2021_01_07_425794 137 39 each each DT 10_1101-2021_01_07_425794 137 40 gene gene NN 10_1101-2021_01_07_425794 137 41 in in IN 10_1101-2021_01_07_425794 137 42 each each DT 10_1101-2021_01_07_425794 137 43 annotation annotation NN 10_1101-2021_01_07_425794 137 44 using use VBG 10_1101-2021_01_07_425794 137 45 the the DT 10_1101-2021_01_07_425794 137 46 featureCounts featurecount NNS 10_1101-2021_01_07_425794 137 47 program program NN 10_1101-2021_01_07_425794 137 48 [ [ -LRB- 10_1101-2021_01_07_425794 137 49 4 4 CD 10_1101-2021_01_07_425794 137 50 , , , 10_1101-2021_01_07_425794 137 51 5 5 CD 10_1101-2021_01_07_425794 137 52 ] ] -RRB- 10_1101-2021_01_07_425794 137 53 . . . 10_1101-2021_01_07_425794 138 1 FeatureCounts FeatureCounts NNP 10_1101-2021_01_07_425794 138 2 assigns assign VBZ 10_1101-2021_01_07_425794 138 3 a a DT 10_1101-2021_01_07_425794 138 4 mapped map VBN 10_1101-2021_01_07_425794 138 5 fragment fragment NN 10_1101-2021_01_07_425794 138 6 to to IN 10_1101-2021_01_07_425794 138 7 a a DT 10_1101-2021_01_07_425794 138 8 gene gene NN 10_1101-2021_01_07_425794 138 9 if if IN 10_1101-2021_01_07_425794 138 10 the the DT 10_1101-2021_01_07_425794 138 11 fragment fragment NN 10_1101-2021_01_07_425794 138 12 overlaps overlap VBZ 10_1101-2021_01_07_425794 138 13 any any DT 10_1101-2021_01_07_425794 138 14 of of IN 10_1101-2021_01_07_425794 138 15 the the DT 10_1101-2021_01_07_425794 138 16 exons exon NNS 10_1101-2021_01_07_425794 138 17 in in IN 10_1101-2021_01_07_425794 138 18 the the DT 10_1101-2021_01_07_425794 138 19 gene gene NN 10_1101-2021_01_07_425794 138 20 . . . 10_1101-2021_01_07_425794 139 1 Figure figure NN 10_1101-2021_01_07_425794 139 2 2 2 CD 10_1101-2021_01_07_425794 139 3 shows show VBZ 10_1101-2021_01_07_425794 139 4 that that IN 10_1101-2021_01_07_425794 139 5 across across IN 10_1101-2021_01_07_425794 139 6 all all PDT 10_1101-2021_01_07_425794 139 7 the the DT 10_1101-2021_01_07_425794 139 8 16 16 CD 10_1101-2021_01_07_425794 139 9 libraries library NNS 10_1101-2021_01_07_425794 139 10 , , , 10_1101-2021_01_07_425794 139 11 the the DT 10_1101-2021_01_07_425794 139 12 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 139 13 - - HYPH 10_1101-2021_01_07_425794 139 14 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 139 15 annotation annotation NN 10_1101-2021_01_07_425794 139 16 constantly constantly RB 10_1101-2021_01_07_425794 139 17 has have VBZ 10_1101-2021_01_07_425794 139 18 substantially substantially RB 10_1101-2021_01_07_425794 139 19 more more JJR 10_1101-2021_01_07_425794 139 20 fragments fragment NNS 10_1101-2021_01_07_425794 139 21 assigned assign VBN 10_1101-2021_01_07_425794 139 22 to to IN 10_1101-2021_01_07_425794 139 23 it -PRON- PRP 10_1101-2021_01_07_425794 139 24 than than IN 10_1101-2021_01_07_425794 139 25 the the DT 10_1101-2021_01_07_425794 139 26 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 139 27 and and CC 10_1101-2021_01_07_425794 139 28 RefSeq- RefSeq- NNP 10_1101-2021_01_07_425794 139 29 NCBI NCBI NNP 10_1101-2021_01_07_425794 139 30 annotations annotation NNS 10_1101-2021_01_07_425794 139 31 . . . 10_1101-2021_01_07_425794 140 1 This this DT 10_1101-2021_01_07_425794 140 2 is be VBZ 10_1101-2021_01_07_425794 140 3 surprising surprising JJ 10_1101-2021_01_07_425794 140 4 because because IN 10_1101-2021_01_07_425794 140 5 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 140 6 - - HYPH 10_1101-2021_01_07_425794 140 7 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 140 8 contains contain VBZ 10_1101-2021_01_07_425794 140 9 much much RB 10_1101-2021_01_07_425794 140 10 less less RBR 10_1101-2021_01_07_425794 140 11 an- an- CC 10_1101-2021_01_07_425794 140 12 notated notate VBN 10_1101-2021_01_07_425794 140 13 genes gene NNS 10_1101-2021_01_07_425794 140 14 and and CC 10_1101-2021_01_07_425794 140 15 also also RB 10_1101-2021_01_07_425794 140 16 has have VBZ 10_1101-2021_01_07_425794 140 17 a a DT 10_1101-2021_01_07_425794 140 18 significantly significantly RB 10_1101-2021_01_07_425794 140 19 smaller small JJR 10_1101-2021_01_07_425794 140 20 transcriptome transcriptome DT 10_1101-2021_01_07_425794 140 21 , , , 10_1101-2021_01_07_425794 140 22 compared compare VBN 10_1101-2021_01_07_425794 140 23 to to IN 10_1101-2021_01_07_425794 140 24 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 140 25 9 9 CD 10_1101-2021_01_07_425794 140 26 .CC .cc SYM 10_1101-2021_01_07_425794 140 27 - - HYPH 10_1101-2021_01_07_425794 140 28 BY by IN 10_1101-2021_01_07_425794 140 29 - - HYPH 10_1101-2021_01_07_425794 140 30 NC NC NNP 10_1101-2021_01_07_425794 140 31 - - HYPH 10_1101-2021_01_07_425794 140 32 ND ND NNP 10_1101-2021_01_07_425794 140 33 4.0 4.0 CD 10_1101-2021_01_07_425794 140 34 International international JJ 10_1101-2021_01_07_425794 140 35 licensemade licensemade NN 10_1101-2021_01_07_425794 140 36 available available JJ 10_1101-2021_01_07_425794 140 37 under under IN 10_1101-2021_01_07_425794 140 38 a a DT 10_1101-2021_01_07_425794 140 39 ( ( -LRB- 10_1101-2021_01_07_425794 140 40 which which WDT 10_1101-2021_01_07_425794 140 41 was be VBD 10_1101-2021_01_07_425794 140 42 not not RB 10_1101-2021_01_07_425794 140 43 certified certify VBN 10_1101-2021_01_07_425794 140 44 by by IN 10_1101-2021_01_07_425794 140 45 peer peer NN 10_1101-2021_01_07_425794 140 46 review review NN 10_1101-2021_01_07_425794 140 47 ) ) -RRB- 10_1101-2021_01_07_425794 140 48 is be VBZ 10_1101-2021_01_07_425794 140 49 the the DT 10_1101-2021_01_07_425794 140 50 author author NN 10_1101-2021_01_07_425794 140 51 / / SYM 10_1101-2021_01_07_425794 140 52 funder funder NN 10_1101-2021_01_07_425794 140 53 , , , 10_1101-2021_01_07_425794 140 54 who who WP 10_1101-2021_01_07_425794 140 55 has have VBZ 10_1101-2021_01_07_425794 140 56 granted grant VBN 10_1101-2021_01_07_425794 140 57 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 140 58 a a DT 10_1101-2021_01_07_425794 140 59 license license NN 10_1101-2021_01_07_425794 140 60 to to TO 10_1101-2021_01_07_425794 140 61 display display VB 10_1101-2021_01_07_425794 140 62 the the DT 10_1101-2021_01_07_425794 140 63 preprint preprint NN 10_1101-2021_01_07_425794 140 64 in in IN 10_1101-2021_01_07_425794 140 65 perpetuity perpetuity NN 10_1101-2021_01_07_425794 140 66 . . . 10_1101-2021_01_07_425794 141 1 It -PRON- PRP 10_1101-2021_01_07_425794 141 2 is be VBZ 10_1101-2021_01_07_425794 141 3 The the DT 10_1101-2021_01_07_425794 141 4 copyright copyright NN 10_1101-2021_01_07_425794 141 5 holder holder NN 10_1101-2021_01_07_425794 141 6 for for IN 10_1101-2021_01_07_425794 141 7 this this DT 10_1101-2021_01_07_425794 141 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 141 9 version version NN 10_1101-2021_01_07_425794 141 10 posted post VBD 10_1101-2021_01_07_425794 141 11 January January NNP 10_1101-2021_01_07_425794 141 12 8 8 CD 10_1101-2021_01_07_425794 141 13 , , , 10_1101-2021_01_07_425794 141 14 2021 2021 CD 10_1101-2021_01_07_425794 141 15 . . . 10_1101-2021_01_07_425794 141 16 ; ; : 10_1101-2021_01_07_425794 141 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 141 18 : : : 10_1101-2021_01_07_425794 141 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 141 20 preprint preprint JJ 10_1101-2021_01_07_425794 141 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 141 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 141 23 P p NN 10_1101-2021_01_07_425794 141 24 er er UH 10_1101-2021_01_07_425794 141 25 ce ce UH 10_1101-2021_01_07_425794 141 26 nt nt RB 10_1101-2021_01_07_425794 141 27 ag ag NNP 10_1101-2021_01_07_425794 141 28 e e NNP 10_1101-2021_01_07_425794 141 29 of of IN 10_1101-2021_01_07_425794 141 30 co co NNP 10_1101-2021_01_07_425794 141 31 un un NNP 10_1101-2021_01_07_425794 141 32 te te NNP 10_1101-2021_01_07_425794 141 33 d d NNP 10_1101-2021_01_07_425794 141 34 fr fr NNP 10_1101-2021_01_07_425794 141 35 ag ag NNP 10_1101-2021_01_07_425794 141 36 m m NNP 10_1101-2021_01_07_425794 141 37 en en NNP 10_1101-2021_01_07_425794 141 38 ts ts NNP 10_1101-2021_01_07_425794 141 39 Key Key NNP 10_1101-2021_01_07_425794 141 40 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 141 41 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 141 42 - - HYPH 10_1101-2021_01_07_425794 141 43 NCBI NCBI NNP 10_1101-2021_01_07_425794 141 44 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 141 45 - - HYPH 10_1101-2021_01_07_425794 141 46 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 141 47 0 0 CD 10_1101-2021_01_07_425794 141 48 20 20 CD 10_1101-2021_01_07_425794 141 49 40 40 CD 10_1101-2021_01_07_425794 141 50 60 60 CD 10_1101-2021_01_07_425794 141 51 80 80 CD 10_1101-2021_01_07_425794 141 52 A a NN 10_1101-2021_01_07_425794 141 53 −1 −1 NNP 10_1101-2021_01_07_425794 141 54 A A NNP 10_1101-2021_01_07_425794 141 55 −2 −2 NNP 10_1101-2021_01_07_425794 141 56 A a NN 10_1101-2021_01_07_425794 141 57 −3 −3 NNP 10_1101-2021_01_07_425794 141 58 A a NN 10_1101-2021_01_07_425794 141 59 −4 −4 NNP 10_1101-2021_01_07_425794 141 60 B B NNP 10_1101-2021_01_07_425794 141 61 −4 −4 NNP 10_1101-2021_01_07_425794 141 62 B B NNP 10_1101-2021_01_07_425794 141 63 −1 −1 NNP 10_1101-2021_01_07_425794 141 64 B B NNP 10_1101-2021_01_07_425794 141 65 −2 −2 NNP 10_1101-2021_01_07_425794 141 66 B B NNP 10_1101-2021_01_07_425794 141 67 −3 −3 NNP 10_1101-2021_01_07_425794 141 68 C C NNP 10_1101-2021_01_07_425794 141 69 −1 −1 NNP 10_1101-2021_01_07_425794 141 70 C C NNP 10_1101-2021_01_07_425794 141 71 −2 −2 NNP 10_1101-2021_01_07_425794 141 72 C C NNP 10_1101-2021_01_07_425794 141 73 −3 −3 NNP 10_1101-2021_01_07_425794 141 74 C C NNP 10_1101-2021_01_07_425794 141 75 −4 −4 NNP 10_1101-2021_01_07_425794 141 76 D D NNP 10_1101-2021_01_07_425794 141 77 −1 −1 NNP 10_1101-2021_01_07_425794 141 78 D D NNP 10_1101-2021_01_07_425794 141 79 −2 −2 NNP 10_1101-2021_01_07_425794 141 80 D D NNP 10_1101-2021_01_07_425794 141 81 −3 −3 NNP 10_1101-2021_01_07_425794 141 82 D D NNP 10_1101-2021_01_07_425794 141 83 −4 −4 NNP 10_1101-2021_01_07_425794 141 84 Figure figure NN 10_1101-2021_01_07_425794 141 85 2 2 CD 10_1101-2021_01_07_425794 141 86 : : : 10_1101-2021_01_07_425794 141 87 Barplots barplot NNS 10_1101-2021_01_07_425794 141 88 showing show VBG 10_1101-2021_01_07_425794 141 89 the the DT 10_1101-2021_01_07_425794 141 90 percentage percentage NN 10_1101-2021_01_07_425794 141 91 of of IN 10_1101-2021_01_07_425794 141 92 fragments fragment NNS 10_1101-2021_01_07_425794 141 93 successfully successfully RB 10_1101-2021_01_07_425794 141 94 assigned assign VBN 10_1101-2021_01_07_425794 141 95 to to IN 10_1101-2021_01_07_425794 141 96 genes gene NNS 10_1101-2021_01_07_425794 141 97 in in IN 10_1101-2021_01_07_425794 141 98 each each DT 10_1101-2021_01_07_425794 141 99 annota- annota- JJ 10_1101-2021_01_07_425794 141 100 tion tion NN 10_1101-2021_01_07_425794 141 101 , , , 10_1101-2021_01_07_425794 141 102 out out IN 10_1101-2021_01_07_425794 141 103 of of IN 10_1101-2021_01_07_425794 141 104 all all PDT 10_1101-2021_01_07_425794 141 105 the the DT 10_1101-2021_01_07_425794 141 106 fragments fragment NNS 10_1101-2021_01_07_425794 141 107 included include VBN 10_1101-2021_01_07_425794 141 108 in in IN 10_1101-2021_01_07_425794 141 109 each each DT 10_1101-2021_01_07_425794 141 110 library library NN 10_1101-2021_01_07_425794 141 111 . . . 10_1101-2021_01_07_425794 142 1 The the DT 10_1101-2021_01_07_425794 142 2 horizontal horizontal JJ 10_1101-2021_01_07_425794 142 3 axis axi NNS 10_1101-2021_01_07_425794 142 4 represents represent VBZ 10_1101-2021_01_07_425794 142 5 the the DT 10_1101-2021_01_07_425794 142 6 sixteen sixteen CD 10_1101-2021_01_07_425794 142 7 SEQC SEQC NNP 10_1101-2021_01_07_425794 142 8 RNA RNA NNP 10_1101-2021_01_07_425794 142 9 - - HYPH 10_1101-2021_01_07_425794 142 10 seq seq NN 10_1101-2021_01_07_425794 142 11 libraries library NNS 10_1101-2021_01_07_425794 142 12 generated generate VBN 10_1101-2021_01_07_425794 142 13 from from IN 10_1101-2021_01_07_425794 142 14 the the DT 10_1101-2021_01_07_425794 142 15 four four CD 10_1101-2021_01_07_425794 142 16 samples sample NNS 10_1101-2021_01_07_425794 142 17 ‘ ' `` 10_1101-2021_01_07_425794 142 18 A a NN 10_1101-2021_01_07_425794 142 19 ’ ' '' 10_1101-2021_01_07_425794 142 20 , , , 10_1101-2021_01_07_425794 142 21 ‘ ' `` 10_1101-2021_01_07_425794 142 22 B b NN 10_1101-2021_01_07_425794 142 23 ’ ' '' 10_1101-2021_01_07_425794 142 24 , , , 10_1101-2021_01_07_425794 142 25 ‘ ' `` 10_1101-2021_01_07_425794 142 26 C C NNP 10_1101-2021_01_07_425794 142 27 ’ ' '' 10_1101-2021_01_07_425794 142 28 and and CC 10_1101-2021_01_07_425794 142 29 ‘ ' `` 10_1101-2021_01_07_425794 142 30 D d NN 10_1101-2021_01_07_425794 142 31 ’ ' '' 10_1101-2021_01_07_425794 142 32 . . . 10_1101-2021_01_07_425794 143 1 Each each DT 10_1101-2021_01_07_425794 143 2 sample sample NN 10_1101-2021_01_07_425794 143 3 has have VBZ 10_1101-2021_01_07_425794 143 4 four four CD 10_1101-2021_01_07_425794 143 5 replicates replicate NNS 10_1101-2021_01_07_425794 143 6 that that WDT 10_1101-2021_01_07_425794 143 7 are be VBP 10_1101-2021_01_07_425794 143 8 numbered number VBN 10_1101-2021_01_07_425794 143 9 from from IN 10_1101-2021_01_07_425794 143 10 1 1 CD 10_1101-2021_01_07_425794 143 11 to to IN 10_1101-2021_01_07_425794 143 12 4 4 CD 10_1101-2021_01_07_425794 143 13 . . . 10_1101-2021_01_07_425794 143 14 and and CC 10_1101-2021_01_07_425794 143 15 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 143 16 - - HYPH 10_1101-2021_01_07_425794 143 17 NCBI NCBI NNP 10_1101-2021_01_07_425794 143 18 ( ( -LRB- 10_1101-2021_01_07_425794 143 19 Figure Figure NNP 10_1101-2021_01_07_425794 143 20 1A 1a NN 10_1101-2021_01_07_425794 143 21 , , , 10_1101-2021_01_07_425794 143 22 D d NN 10_1101-2021_01_07_425794 143 23 ) ) -RRB- 10_1101-2021_01_07_425794 143 24 . . . 10_1101-2021_01_07_425794 144 1 We -PRON- PRP 10_1101-2021_01_07_425794 144 2 then then RB 10_1101-2021_01_07_425794 144 3 performed perform VBD 10_1101-2021_01_07_425794 144 4 a a DT 10_1101-2021_01_07_425794 144 5 detailed detailed JJ 10_1101-2021_01_07_425794 144 6 investigation investigation NN 10_1101-2021_01_07_425794 144 7 into into IN 10_1101-2021_01_07_425794 144 8 the the DT 10_1101-2021_01_07_425794 144 9 mapping mapping NN 10_1101-2021_01_07_425794 144 10 and and CC 10_1101-2021_01_07_425794 144 11 counting counting NN 10_1101-2021_01_07_425794 144 12 results result NNS 10_1101-2021_01_07_425794 144 13 to to TO 10_1101-2021_01_07_425794 144 14 find find VB 10_1101-2021_01_07_425794 144 15 out out RP 10_1101-2021_01_07_425794 144 16 what what WP 10_1101-2021_01_07_425794 144 17 enabled enable VBD 10_1101-2021_01_07_425794 144 18 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 144 19 - - HYPH 10_1101-2021_01_07_425794 144 20 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 144 21 to to TO 10_1101-2021_01_07_425794 144 22 achieve achieve VB 10_1101-2021_01_07_425794 144 23 a a DT 10_1101-2021_01_07_425794 144 24 higher high JJR 10_1101-2021_01_07_425794 144 25 percentage percentage NN 10_1101-2021_01_07_425794 144 26 of of IN 10_1101-2021_01_07_425794 144 27 successfully successfully RB 10_1101-2021_01_07_425794 144 28 assigned assign VBN 10_1101-2021_01_07_425794 144 29 fragments fragment NNS 10_1101-2021_01_07_425794 144 30 . . . 10_1101-2021_01_07_425794 145 1 Although although IN 10_1101-2021_01_07_425794 145 2 gene gene NN 10_1101-2021_01_07_425794 145 3 annotations annotation NNS 10_1101-2021_01_07_425794 145 4 were be VBD 10_1101-2021_01_07_425794 145 5 utilized utilize VBN 10_1101-2021_01_07_425794 145 6 in in IN 10_1101-2021_01_07_425794 145 7 mapping mapping NN 10_1101-2021_01_07_425794 145 8 reads read VBZ 10_1101-2021_01_07_425794 145 9 to to IN 10_1101-2021_01_07_425794 145 10 the the DT 10_1101-2021_01_07_425794 145 11 human human JJ 10_1101-2021_01_07_425794 145 12 reference reference NN 10_1101-2021_01_07_425794 145 13 genome genome NN 10_1101-2021_01_07_425794 145 14 , , , 10_1101-2021_01_07_425794 145 15 the the DT 10_1101-2021_01_07_425794 145 16 use use NN 10_1101-2021_01_07_425794 145 17 of of IN 10_1101-2021_01_07_425794 145 18 different different JJ 10_1101-2021_01_07_425794 145 19 annotations annotation NNS 10_1101-2021_01_07_425794 145 20 was be VBD 10_1101-2021_01_07_425794 145 21 not not RB 10_1101-2021_01_07_425794 145 22 found find VBN 10_1101-2021_01_07_425794 145 23 to to TO 10_1101-2021_01_07_425794 145 24 affect affect VB 10_1101-2021_01_07_425794 145 25 the the DT 10_1101-2021_01_07_425794 145 26 number number NN 10_1101-2021_01_07_425794 145 27 of of IN 10_1101-2021_01_07_425794 145 28 success- success- NN 10_1101-2021_01_07_425794 145 29 fully fully RB 10_1101-2021_01_07_425794 145 30 aligned align VBN 10_1101-2021_01_07_425794 145 31 fragments fragment NNS 10_1101-2021_01_07_425794 145 32 for for IN 10_1101-2021_01_07_425794 145 33 each each DT 10_1101-2021_01_07_425794 145 34 library library NN 10_1101-2021_01_07_425794 145 35 ( ( -LRB- 10_1101-2021_01_07_425794 145 36 Supplementary Supplementary NNP 10_1101-2021_01_07_425794 145 37 Figure Figure NNP 10_1101-2021_01_07_425794 145 38 S1 S1 NNS 10_1101-2021_01_07_425794 145 39 ) ) -RRB- 10_1101-2021_01_07_425794 145 40 . . . 10_1101-2021_01_07_425794 146 1 We -PRON- PRP 10_1101-2021_01_07_425794 146 2 found find VBD 10_1101-2021_01_07_425794 146 3 that that IN 10_1101-2021_01_07_425794 146 4 when when WRB 10_1101-2021_01_07_425794 146 5 assigning assign VBG 10_1101-2021_01_07_425794 146 6 fragments fragment NNS 10_1101-2021_01_07_425794 146 7 to to IN 10_1101-2021_01_07_425794 146 8 genes gene NNS 10_1101-2021_01_07_425794 146 9 using use VBG 10_1101-2021_01_07_425794 146 10 the the DT 10_1101-2021_01_07_425794 146 11 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 146 12 or or CC 10_1101-2021_01_07_425794 146 13 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 146 14 - - HYPH 10_1101-2021_01_07_425794 146 15 NCBI NCBI NNP 10_1101-2021_01_07_425794 146 16 annotation annotation NN 10_1101-2021_01_07_425794 146 17 , , , 10_1101-2021_01_07_425794 146 18 more more RBR 10_1101-2021_01_07_425794 146 19 frag- frag- NN 10_1101-2021_01_07_425794 146 20 ments ment NNS 10_1101-2021_01_07_425794 146 21 were be VBD 10_1101-2021_01_07_425794 146 22 unable unable JJ 10_1101-2021_01_07_425794 146 23 to to TO 10_1101-2021_01_07_425794 146 24 be be VB 10_1101-2021_01_07_425794 146 25 assigned assign VBN 10_1101-2021_01_07_425794 146 26 because because IN 10_1101-2021_01_07_425794 146 27 they -PRON- PRP 10_1101-2021_01_07_425794 146 28 did do VBD 10_1101-2021_01_07_425794 146 29 not not RB 10_1101-2021_01_07_425794 146 30 overlap overlap VB 10_1101-2021_01_07_425794 146 31 any any DT 10_1101-2021_01_07_425794 146 32 genes gene NNS 10_1101-2021_01_07_425794 146 33 ( ( -LRB- 10_1101-2021_01_07_425794 146 34 ie ie FW 10_1101-2021_01_07_425794 146 35 . . . 10_1101-2021_01_07_425794 147 1 failed fail VBD 10_1101-2021_01_07_425794 147 2 to to TO 10_1101-2021_01_07_425794 147 3 overlap overlap VB 10_1101-2021_01_07_425794 147 4 any any DT 10_1101-2021_01_07_425794 147 5 exons exon NNS 10_1101-2021_01_07_425794 147 6 included include VBN 10_1101-2021_01_07_425794 147 7 in in IN 10_1101-2021_01_07_425794 147 8 any any DT 10_1101-2021_01_07_425794 147 9 genes gene NNS 10_1101-2021_01_07_425794 147 10 ) ) -RRB- 10_1101-2021_01_07_425794 147 11 , , , 10_1101-2021_01_07_425794 147 12 despite despite IN 10_1101-2021_01_07_425794 147 13 there there EX 10_1101-2021_01_07_425794 147 14 are be VBP 10_1101-2021_01_07_425794 147 15 more more JJR 10_1101-2021_01_07_425794 147 16 genes gene NNS 10_1101-2021_01_07_425794 147 17 included include VBN 10_1101-2021_01_07_425794 147 18 in in IN 10_1101-2021_01_07_425794 147 19 these these DT 10_1101-2021_01_07_425794 147 20 annotations annotation NNS 10_1101-2021_01_07_425794 147 21 compared compare VBN 10_1101-2021_01_07_425794 147 22 to to IN 10_1101-2021_01_07_425794 147 23 the the DT 10_1101-2021_01_07_425794 147 24 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 147 25 - - HYPH 10_1101-2021_01_07_425794 147 26 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 147 27 annotation annotation NN 10_1101-2021_01_07_425794 147 28 ( ( -LRB- 10_1101-2021_01_07_425794 147 29 Supplementary Supplementary NNP 10_1101-2021_01_07_425794 147 30 Figure Figure NNP 10_1101-2021_01_07_425794 147 31 S2 S2 NNP 10_1101-2021_01_07_425794 147 32 ) ) -RRB- 10_1101-2021_01_07_425794 147 33 . . . 10_1101-2021_01_07_425794 148 1 This this DT 10_1101-2021_01_07_425794 148 2 is be VBZ 10_1101-2021_01_07_425794 148 3 particularly particularly RB 10_1101-2021_01_07_425794 148 4 the the DT 10_1101-2021_01_07_425794 148 5 case case NN 10_1101-2021_01_07_425794 148 6 for for IN 10_1101-2021_01_07_425794 148 7 the the DT 10_1101-2021_01_07_425794 148 8 fragment fragment NN 10_1101-2021_01_07_425794 148 9 assignment assignment NN 10_1101-2021_01_07_425794 148 10 in in IN 10_1101-2021_01_07_425794 148 11 the the DT 10_1101-2021_01_07_425794 148 12 human human JJ 10_1101-2021_01_07_425794 148 13 brain brain NN 10_1101-2021_01_07_425794 148 14 reference reference NN 10_1101-2021_01_07_425794 148 15 samples sample NNS 10_1101-2021_01_07_425794 148 16 . . . 10_1101-2021_01_07_425794 149 1 We -PRON- PRP 10_1101-2021_01_07_425794 149 2 also also RB 10_1101-2021_01_07_425794 149 3 found find VBD 10_1101-2021_01_07_425794 149 4 that that IN 10_1101-2021_01_07_425794 149 5 the the DT 10_1101-2021_01_07_425794 149 6 use use NN 10_1101-2021_01_07_425794 149 7 of of IN 10_1101-2021_01_07_425794 149 8 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 149 9 and and CC 10_1101-2021_01_07_425794 149 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 149 11 - - HYPH 10_1101-2021_01_07_425794 149 12 NCBI NCBI NNP 10_1101-2021_01_07_425794 149 13 annotations annotation NNS 10_1101-2021_01_07_425794 149 14 led lead VBD 10_1101-2021_01_07_425794 149 15 to to IN 10_1101-2021_01_07_425794 149 16 more more JJR 10_1101-2021_01_07_425794 149 17 fragments fragment NNS 10_1101-2021_01_07_425794 149 18 being be VBG 10_1101-2021_01_07_425794 149 19 unassigned unassigne VBN 10_1101-2021_01_07_425794 149 20 due due IN 10_1101-2021_01_07_425794 149 21 to to IN 10_1101-2021_01_07_425794 149 22 the the DT 10_1101-2021_01_07_425794 149 23 assignment assignment NN 10_1101-2021_01_07_425794 149 24 ambiguity ambiguity NN 10_1101-2021_01_07_425794 149 25 , , , 10_1101-2021_01_07_425794 149 26 ie ie NNP 10_1101-2021_01_07_425794 149 27 . . . 10_1101-2021_01_07_425794 150 1 a a DT 10_1101-2021_01_07_425794 150 2 fragment fragment NN 10_1101-2021_01_07_425794 150 3 over- over- NN 10_1101-2021_01_07_425794 150 4 laps lap VBZ 10_1101-2021_01_07_425794 150 5 more more JJR 10_1101-2021_01_07_425794 150 6 than than IN 10_1101-2021_01_07_425794 150 7 one one CD 10_1101-2021_01_07_425794 150 8 gene gene NN 10_1101-2021_01_07_425794 150 9 ( ( -LRB- 10_1101-2021_01_07_425794 150 10 Supplementary Supplementary NNP 10_1101-2021_01_07_425794 150 11 Figure Figure NNP 10_1101-2021_01_07_425794 150 12 S3 S3 NNP 10_1101-2021_01_07_425794 150 13 ) ) -RRB- 10_1101-2021_01_07_425794 150 14 . . . 10_1101-2021_01_07_425794 151 1 This this DT 10_1101-2021_01_07_425794 151 2 should should MD 10_1101-2021_01_07_425794 151 3 be be VB 10_1101-2021_01_07_425794 151 4 because because IN 10_1101-2021_01_07_425794 151 5 there there EX 10_1101-2021_01_07_425794 151 6 are be VBP 10_1101-2021_01_07_425794 151 7 more more JJR 10_1101-2021_01_07_425794 151 8 genes gene NNS 10_1101-2021_01_07_425794 151 9 that that WDT 10_1101-2021_01_07_425794 151 10 overlap overlap VBP 10_1101-2021_01_07_425794 151 11 with with IN 10_1101-2021_01_07_425794 151 12 each each DT 10_1101-2021_01_07_425794 151 13 other other JJ 10_1101-2021_01_07_425794 151 14 ( ( -LRB- 10_1101-2021_01_07_425794 151 15 ie ie FW 10_1101-2021_01_07_425794 151 16 . . . 10_1101-2021_01_07_425794 152 1 exons exon NNS 10_1101-2021_01_07_425794 152 2 from from IN 10_1101-2021_01_07_425794 152 3 different different JJ 10_1101-2021_01_07_425794 152 4 genes gene NNS 10_1101-2021_01_07_425794 152 5 overlap overlap VBP 10_1101-2021_01_07_425794 152 6 with with IN 10_1101-2021_01_07_425794 152 7 each each DT 10_1101-2021_01_07_425794 152 8 other other JJ 10_1101-2021_01_07_425794 152 9 ) ) -RRB- 10_1101-2021_01_07_425794 152 10 in in IN 10_1101-2021_01_07_425794 152 11 the the DT 10_1101-2021_01_07_425794 152 12 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 152 13 and and CC 10_1101-2021_01_07_425794 152 14 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 152 15 - - HYPH 10_1101-2021_01_07_425794 152 16 NCBI NCBI NNP 10_1101-2021_01_07_425794 152 17 annotations annotation NNS 10_1101-2021_01_07_425794 152 18 compared compare VBN 10_1101-2021_01_07_425794 152 19 to to IN 10_1101-2021_01_07_425794 152 20 the the DT 10_1101-2021_01_07_425794 152 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 152 22 - - HYPH 10_1101-2021_01_07_425794 152 23 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 152 24 annotation annotation NN 10_1101-2021_01_07_425794 152 25 . . . 10_1101-2021_01_07_425794 153 1 Our -PRON- PRP$ 10_1101-2021_01_07_425794 153 2 investigation investigation NN 10_1101-2021_01_07_425794 153 3 revealed reveal VBD 10_1101-2021_01_07_425794 153 4 that that IN 10_1101-2021_01_07_425794 153 5 less less JJR 10_1101-2021_01_07_425794 153 6 gene gene NN 10_1101-2021_01_07_425794 153 7 overlapping overlapping NN 10_1101-2021_01_07_425794 153 8 in in IN 10_1101-2021_01_07_425794 153 9 the the DT 10_1101-2021_01_07_425794 153 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 153 11 - - HYPH 10_1101-2021_01_07_425794 153 12 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 153 13 annotation annotation NN 10_1101-2021_01_07_425794 153 14 and and CC 10_1101-2021_01_07_425794 153 15 better well JJR 10_1101-2021_01_07_425794 153 16 compatibility compatibility NN 10_1101-2021_01_07_425794 153 17 of of IN 10_1101-2021_01_07_425794 153 18 this this DT 10_1101-2021_01_07_425794 153 19 annotation annotation NN 10_1101-2021_01_07_425794 153 20 with with IN 10_1101-2021_01_07_425794 153 21 the the DT 10_1101-2021_01_07_425794 153 22 mapped map VBN 10_1101-2021_01_07_425794 153 23 fragments fragment NNS 10_1101-2021_01_07_425794 153 24 have have VBP 10_1101-2021_01_07_425794 153 25 led lead VBN 10_1101-2021_01_07_425794 153 26 to to IN 10_1101-2021_01_07_425794 153 27 more more JJR 10_1101-2021_01_07_425794 153 28 fragments fragment NNS 10_1101-2021_01_07_425794 153 29 being be VBG 10_1101-2021_01_07_425794 153 30 successfully successfully RB 10_1101-2021_01_07_425794 153 31 counted count VBN 10_1101-2021_01_07_425794 153 32 for for IN 10_1101-2021_01_07_425794 153 33 each each DT 10_1101-2021_01_07_425794 153 34 library library NN 10_1101-2021_01_07_425794 153 35 in in IN 10_1101-2021_01_07_425794 153 36 this this DT 10_1101-2021_01_07_425794 153 37 dataset dataset NN 10_1101-2021_01_07_425794 153 38 . . . 10_1101-2021_01_07_425794 154 1 Given give VBN 10_1101-2021_01_07_425794 154 2 10 10 CD 10_1101-2021_01_07_425794 154 3 .CC .CC , 10_1101-2021_01_07_425794 154 4 - - HYPH 10_1101-2021_01_07_425794 154 5 BY by IN 10_1101-2021_01_07_425794 154 6 - - HYPH 10_1101-2021_01_07_425794 154 7 NC NC NNP 10_1101-2021_01_07_425794 154 8 - - HYPH 10_1101-2021_01_07_425794 154 9 ND ND NNP 10_1101-2021_01_07_425794 154 10 4.0 4.0 CD 10_1101-2021_01_07_425794 154 11 International international JJ 10_1101-2021_01_07_425794 154 12 licensemade licensemade NN 10_1101-2021_01_07_425794 154 13 available available JJ 10_1101-2021_01_07_425794 154 14 under under IN 10_1101-2021_01_07_425794 154 15 a a DT 10_1101-2021_01_07_425794 154 16 ( ( -LRB- 10_1101-2021_01_07_425794 154 17 which which WDT 10_1101-2021_01_07_425794 154 18 was be VBD 10_1101-2021_01_07_425794 154 19 not not RB 10_1101-2021_01_07_425794 154 20 certified certify VBN 10_1101-2021_01_07_425794 154 21 by by IN 10_1101-2021_01_07_425794 154 22 peer peer NN 10_1101-2021_01_07_425794 154 23 review review NN 10_1101-2021_01_07_425794 154 24 ) ) -RRB- 10_1101-2021_01_07_425794 154 25 is be VBZ 10_1101-2021_01_07_425794 154 26 the the DT 10_1101-2021_01_07_425794 154 27 author author NN 10_1101-2021_01_07_425794 154 28 / / SYM 10_1101-2021_01_07_425794 154 29 funder funder NN 10_1101-2021_01_07_425794 154 30 , , , 10_1101-2021_01_07_425794 154 31 who who WP 10_1101-2021_01_07_425794 154 32 has have VBZ 10_1101-2021_01_07_425794 154 33 granted grant VBN 10_1101-2021_01_07_425794 154 34 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 154 35 a a DT 10_1101-2021_01_07_425794 154 36 license license NN 10_1101-2021_01_07_425794 154 37 to to TO 10_1101-2021_01_07_425794 154 38 display display VB 10_1101-2021_01_07_425794 154 39 the the DT 10_1101-2021_01_07_425794 154 40 preprint preprint NN 10_1101-2021_01_07_425794 154 41 in in IN 10_1101-2021_01_07_425794 154 42 perpetuity perpetuity NN 10_1101-2021_01_07_425794 154 43 . . . 10_1101-2021_01_07_425794 155 1 It -PRON- PRP 10_1101-2021_01_07_425794 155 2 is be VBZ 10_1101-2021_01_07_425794 155 3 The the DT 10_1101-2021_01_07_425794 155 4 copyright copyright NN 10_1101-2021_01_07_425794 155 5 holder holder NN 10_1101-2021_01_07_425794 155 6 for for IN 10_1101-2021_01_07_425794 155 7 this this DT 10_1101-2021_01_07_425794 155 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 155 9 version version NN 10_1101-2021_01_07_425794 155 10 posted post VBD 10_1101-2021_01_07_425794 155 11 January January NNP 10_1101-2021_01_07_425794 155 12 8 8 CD 10_1101-2021_01_07_425794 155 13 , , , 10_1101-2021_01_07_425794 155 14 2021 2021 CD 10_1101-2021_01_07_425794 155 15 . . . 10_1101-2021_01_07_425794 155 16 ; ; : 10_1101-2021_01_07_425794 155 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 155 18 : : : 10_1101-2021_01_07_425794 155 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 155 20 preprint preprint JJ 10_1101-2021_01_07_425794 155 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 155 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425794 155 23 −10 −10 NNP 10_1101-2021_01_07_425794 155 24 −5 −5 NNP 10_1101-2021_01_07_425794 155 25 0 0 CD 10_1101-2021_01_07_425794 155 26 5 5 CD 10_1101-2021_01_07_425794 155 27 10 10 CD 10_1101-2021_01_07_425794 155 28 15 15 CD 10_1101-2021_01_07_425794 155 29 lo lo IN 10_1101-2021_01_07_425794 155 30 g2 g2 NNP 10_1101-2021_01_07_425794 155 31 R r NN 10_1101-2021_01_07_425794 155 32 P p NN 10_1101-2021_01_07_425794 155 33 K k NN 10_1101-2021_01_07_425794 155 34 M M NNP 10_1101-2021_01_07_425794 155 35 A A NNP 10_1101-2021_01_07_425794 155 36 −1 −1 NNP 10_1101-2021_01_07_425794 155 37 A A NNP 10_1101-2021_01_07_425794 155 38 −2 −2 NNP 10_1101-2021_01_07_425794 155 39 A a NN 10_1101-2021_01_07_425794 155 40 −3 −3 NNP 10_1101-2021_01_07_425794 155 41 A A NNP 10_1101-2021_01_07_425794 155 42 −4 −4 NNP 10_1101-2021_01_07_425794 155 43 B B NNP 10_1101-2021_01_07_425794 155 44 −1 −1 NNP 10_1101-2021_01_07_425794 155 45 B B NNP 10_1101-2021_01_07_425794 155 46 −2 −2 NNP 10_1101-2021_01_07_425794 155 47 B B NNP 10_1101-2021_01_07_425794 155 48 −3 −3 NNP 10_1101-2021_01_07_425794 155 49 B B NNP 10_1101-2021_01_07_425794 155 50 −4 −4 NNP 10_1101-2021_01_07_425794 155 51 C C NNP 10_1101-2021_01_07_425794 155 52 −1 −1 NNP 10_1101-2021_01_07_425794 155 53 C C NNP 10_1101-2021_01_07_425794 155 54 −2 −2 NNP 10_1101-2021_01_07_425794 155 55 C C NNP 10_1101-2021_01_07_425794 155 56 −3 −3 NNP 10_1101-2021_01_07_425794 155 57 C C NNP 10_1101-2021_01_07_425794 155 58 −4 −4 NNP 10_1101-2021_01_07_425794 155 59 D D NNP 10_1101-2021_01_07_425794 155 60 −1 −1 NNP 10_1101-2021_01_07_425794 155 61 D D NNP 10_1101-2021_01_07_425794 155 62 −2 −2 NNP 10_1101-2021_01_07_425794 155 63 D D NNP 10_1101-2021_01_07_425794 155 64 −3 −3 NNP 10_1101-2021_01_07_425794 155 65 D D NNP 10_1101-2021_01_07_425794 155 66 −4 −4 NNP 10_1101-2021_01_07_425794 155 67 Key Key NNP 10_1101-2021_01_07_425794 155 68 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 155 69 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 155 70 - - HYPH 10_1101-2021_01_07_425794 155 71 NCBI NCBI NNP 10_1101-2021_01_07_425794 155 72 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 155 73 - - HYPH 10_1101-2021_01_07_425794 155 74 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 155 75 Figure Figure NNP 10_1101-2021_01_07_425794 155 76 3 3 CD 10_1101-2021_01_07_425794 155 77 : : : 10_1101-2021_01_07_425794 155 78 Boxplots boxplot NNS 10_1101-2021_01_07_425794 155 79 comparing compare VBG 10_1101-2021_01_07_425794 155 80 the the DT 10_1101-2021_01_07_425794 155 81 intensity intensity NN 10_1101-2021_01_07_425794 155 82 range range NN 10_1101-2021_01_07_425794 155 83 of of IN 10_1101-2021_01_07_425794 155 84 gene gene NN 10_1101-2021_01_07_425794 155 85 expression expression NN 10_1101-2021_01_07_425794 155 86 between between IN 10_1101-2021_01_07_425794 155 87 the the DT 10_1101-2021_01_07_425794 155 88 three three CD 10_1101-2021_01_07_425794 155 89 annotations annotation NNS 10_1101-2021_01_07_425794 155 90 . . . 10_1101-2021_01_07_425794 156 1 All all PDT 10_1101-2021_01_07_425794 156 2 the the DT 10_1101-2021_01_07_425794 156 3 genes gene NNS 10_1101-2021_01_07_425794 156 4 from from IN 10_1101-2021_01_07_425794 156 5 each each DT 10_1101-2021_01_07_425794 156 6 annotation annotation NN 10_1101-2021_01_07_425794 156 7 were be VBD 10_1101-2021_01_07_425794 156 8 included include VBN 10_1101-2021_01_07_425794 156 9 in in IN 10_1101-2021_01_07_425794 156 10 the the DT 10_1101-2021_01_07_425794 156 11 plots plot NNS 10_1101-2021_01_07_425794 156 12 . . . 10_1101-2021_01_07_425794 157 1 Raw raw JJ 10_1101-2021_01_07_425794 157 2 read read VBD 10_1101-2021_01_07_425794 157 3 counts count NNS 10_1101-2021_01_07_425794 157 4 of of IN 10_1101-2021_01_07_425794 157 5 genes gene NNS 10_1101-2021_01_07_425794 157 6 were be VBD 10_1101-2021_01_07_425794 157 7 transformed transform VBN 10_1101-2021_01_07_425794 157 8 to to IN 10_1101-2021_01_07_425794 157 9 log2FPKM log2fpkm CD 10_1101-2021_01_07_425794 157 10 values value NNS 10_1101-2021_01_07_425794 157 11 . . . 10_1101-2021_01_07_425794 158 1 A a DT 10_1101-2021_01_07_425794 158 2 prior prior JJ 10_1101-2021_01_07_425794 158 3 count count NN 10_1101-2021_01_07_425794 158 4 of of IN 10_1101-2021_01_07_425794 158 5 0.5 0.5 CD 10_1101-2021_01_07_425794 158 6 was be VBD 10_1101-2021_01_07_425794 158 7 added add VBN 10_1101-2021_01_07_425794 158 8 to to IN 10_1101-2021_01_07_425794 158 9 raw raw JJ 10_1101-2021_01_07_425794 158 10 counts count NNS 10_1101-2021_01_07_425794 158 11 to to TO 10_1101-2021_01_07_425794 158 12 avoid avoid VB 10_1101-2021_01_07_425794 158 13 log log NN 10_1101-2021_01_07_425794 158 14 - - HYPH 10_1101-2021_01_07_425794 158 15 transformation transformation NN 10_1101-2021_01_07_425794 158 16 of of IN 10_1101-2021_01_07_425794 158 17 zero zero CD 10_1101-2021_01_07_425794 158 18 . . . 10_1101-2021_01_07_425794 159 1 that that IN 10_1101-2021_01_07_425794 159 2 both both CC 10_1101-2021_01_07_425794 159 3 the the DT 10_1101-2021_01_07_425794 159 4 Universal Universal NNP 10_1101-2021_01_07_425794 159 5 Human Human NNP 10_1101-2021_01_07_425794 159 6 Reference Reference NNP 10_1101-2021_01_07_425794 159 7 and and CC 10_1101-2021_01_07_425794 159 8 Human Human NNP 10_1101-2021_01_07_425794 159 9 Brain Brain NNP 10_1101-2021_01_07_425794 159 10 Reference Reference NNP 10_1101-2021_01_07_425794 159 11 samples sample NNS 10_1101-2021_01_07_425794 159 12 used use VBN 10_1101-2021_01_07_425794 159 13 in in IN 10_1101-2021_01_07_425794 159 14 this this DT 10_1101-2021_01_07_425794 159 15 study study NN 10_1101-2021_01_07_425794 159 16 are be VBP 10_1101-2021_01_07_425794 159 17 known know VBN 10_1101-2021_01_07_425794 159 18 to to TO 10_1101-2021_01_07_425794 159 19 contain contain VB 10_1101-2021_01_07_425794 159 20 a a DT 10_1101-2021_01_07_425794 159 21 very very RB 10_1101-2021_01_07_425794 159 22 high high JJ 10_1101-2021_01_07_425794 159 23 number number NN 10_1101-2021_01_07_425794 159 24 of of IN 10_1101-2021_01_07_425794 159 25 expressed express VBN 10_1101-2021_01_07_425794 159 26 genes gene NNS 10_1101-2021_01_07_425794 159 27 and and CC 10_1101-2021_01_07_425794 159 28 the the DT 10_1101-2021_01_07_425794 159 29 RNA RNA NNP 10_1101-2021_01_07_425794 159 30 - - HYPH 10_1101-2021_01_07_425794 159 31 seq seq NN 10_1101-2021_01_07_425794 159 32 data datum NNS 10_1101-2021_01_07_425794 159 33 generated generate VBN 10_1101-2021_01_07_425794 159 34 from from IN 10_1101-2021_01_07_425794 159 35 these these DT 10_1101-2021_01_07_425794 159 36 samples sample NNS 10_1101-2021_01_07_425794 159 37 are be VBP 10_1101-2021_01_07_425794 159 38 expected expect VBN 10_1101-2021_01_07_425794 159 39 to to TO 10_1101-2021_01_07_425794 159 40 cover cover VB 10_1101-2021_01_07_425794 159 41 most most JJS 10_1101-2021_01_07_425794 159 42 of of IN 10_1101-2021_01_07_425794 159 43 the the DT 10_1101-2021_01_07_425794 159 44 human human NN 10_1101-2021_01_07_425794 159 45 transcrip- transcrip- XX 10_1101-2021_01_07_425794 159 46 tome tome NNP 10_1101-2021_01_07_425794 159 47 , , , 10_1101-2021_01_07_425794 159 48 our -PRON- PRP$ 10_1101-2021_01_07_425794 159 49 analysis analysis NN 10_1101-2021_01_07_425794 159 50 suggests suggest VBZ 10_1101-2021_01_07_425794 159 51 that that IN 10_1101-2021_01_07_425794 159 52 the the DT 10_1101-2021_01_07_425794 159 53 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 159 54 - - HYPH 10_1101-2021_01_07_425794 159 55 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 159 56 annotation annotation NN 10_1101-2021_01_07_425794 159 57 is be VBZ 10_1101-2021_01_07_425794 159 58 likely likely JJ 10_1101-2021_01_07_425794 159 59 to to TO 10_1101-2021_01_07_425794 159 60 contain contain VB 10_1101-2021_01_07_425794 159 61 more more JJR 10_1101-2021_01_07_425794 159 62 transcribed transcribe VBN 10_1101-2021_01_07_425794 159 63 region region NN 10_1101-2021_01_07_425794 159 64 in in IN 10_1101-2021_01_07_425794 159 65 the the DT 10_1101-2021_01_07_425794 159 66 genome genome NN 10_1101-2021_01_07_425794 159 67 than than IN 10_1101-2021_01_07_425794 159 68 the the DT 10_1101-2021_01_07_425794 159 69 other other JJ 10_1101-2021_01_07_425794 159 70 two two CD 10_1101-2021_01_07_425794 159 71 annotations annotation NNS 10_1101-2021_01_07_425794 159 72 in in IN 10_1101-2021_01_07_425794 159 73 general general JJ 10_1101-2021_01_07_425794 159 74 . . . 10_1101-2021_01_07_425794 160 1 3.3 3.3 CD 10_1101-2021_01_07_425794 160 2 Intensity intensity NN 10_1101-2021_01_07_425794 160 3 range range NN 10_1101-2021_01_07_425794 160 4 of of IN 10_1101-2021_01_07_425794 160 5 gene gene NN 10_1101-2021_01_07_425794 160 6 expression expression NN 10_1101-2021_01_07_425794 160 7 We -PRON- PRP 10_1101-2021_01_07_425794 160 8 examined examine VBD 10_1101-2021_01_07_425794 160 9 if if IN 10_1101-2021_01_07_425794 160 10 the the DT 10_1101-2021_01_07_425794 160 11 gene gene NN 10_1101-2021_01_07_425794 160 12 annotation annotation NN 10_1101-2021_01_07_425794 160 13 choice choice NN 10_1101-2021_01_07_425794 160 14 has have VBZ 10_1101-2021_01_07_425794 160 15 an an DT 10_1101-2021_01_07_425794 160 16 impact impact NN 10_1101-2021_01_07_425794 160 17 on on IN 10_1101-2021_01_07_425794 160 18 the the DT 10_1101-2021_01_07_425794 160 19 range range NN 10_1101-2021_01_07_425794 160 20 of of IN 10_1101-2021_01_07_425794 160 21 gene gene NN 10_1101-2021_01_07_425794 160 22 expression expression NN 10_1101-2021_01_07_425794 160 23 levels level NNS 10_1101-2021_01_07_425794 160 24 in in IN 10_1101-2021_01_07_425794 160 25 the the DT 10_1101-2021_01_07_425794 160 26 RNA RNA NNP 10_1101-2021_01_07_425794 160 27 - - HYPH 10_1101-2021_01_07_425794 160 28 seq seq NN 10_1101-2021_01_07_425794 160 29 data datum NNS 10_1101-2021_01_07_425794 160 30 . . . 10_1101-2021_01_07_425794 161 1 Raw raw JJ 10_1101-2021_01_07_425794 161 2 gene gene NN 10_1101-2021_01_07_425794 161 3 counts count NNS 10_1101-2021_01_07_425794 161 4 of of IN 10_1101-2021_01_07_425794 161 5 the the DT 10_1101-2021_01_07_425794 161 6 SEQC SEQC NNP 10_1101-2021_01_07_425794 161 7 data datum NNS 10_1101-2021_01_07_425794 161 8 were be VBD 10_1101-2021_01_07_425794 161 9 converted convert VBN 10_1101-2021_01_07_425794 161 10 to to IN 10_1101-2021_01_07_425794 161 11 log2FPKM log2FPKM NNP 10_1101-2021_01_07_425794 161 12 ( ( -LRB- 10_1101-2021_01_07_425794 161 13 log2 log2 NN 10_1101-2021_01_07_425794 161 14 fragments fragment NNS 10_1101-2021_01_07_425794 161 15 per per IN 10_1101-2021_01_07_425794 161 16 kilo kilo NNP 10_1101-2021_01_07_425794 161 17 exonic exonic JJ 10_1101-2021_01_07_425794 161 18 bases basis NNS 10_1101-2021_01_07_425794 161 19 per per IN 10_1101-2021_01_07_425794 161 20 million million CD 10_1101-2021_01_07_425794 161 21 mapped map VBN 10_1101-2021_01_07_425794 161 22 fragments fragment NNS 10_1101-2021_01_07_425794 161 23 ) ) -RRB- 10_1101-2021_01_07_425794 161 24 values value NNS 10_1101-2021_01_07_425794 161 25 for for IN 10_1101-2021_01_07_425794 161 26 all all PDT 10_1101-2021_01_07_425794 161 27 the the DT 10_1101-2021_01_07_425794 161 28 genes gene NNS 10_1101-2021_01_07_425794 161 29 included include VBN 10_1101-2021_01_07_425794 161 30 in in IN 10_1101-2021_01_07_425794 161 31 each each DT 10_1101-2021_01_07_425794 161 32 annotation annotation NN 10_1101-2021_01_07_425794 161 33 . . . 10_1101-2021_01_07_425794 162 1 A a DT 10_1101-2021_01_07_425794 162 2 prior prior JJ 10_1101-2021_01_07_425794 162 3 count count NN 10_1101-2021_01_07_425794 162 4 of of IN 10_1101-2021_01_07_425794 162 5 0.5 0.5 CD 10_1101-2021_01_07_425794 162 6 was be VBD 10_1101-2021_01_07_425794 162 7 added add VBN 10_1101-2021_01_07_425794 162 8 to to IN 10_1101-2021_01_07_425794 162 9 the the DT 10_1101-2021_01_07_425794 162 10 raw raw JJ 10_1101-2021_01_07_425794 162 11 counts count NNS 10_1101-2021_01_07_425794 162 12 to to TO 10_1101-2021_01_07_425794 162 13 avoid avoid VB 10_1101-2021_01_07_425794 162 14 log log NN 10_1101-2021_01_07_425794 162 15 - - HYPH 10_1101-2021_01_07_425794 162 16 transformation transformation NN 10_1101-2021_01_07_425794 162 17 of of IN 10_1101-2021_01_07_425794 162 18 zero zero CD 10_1101-2021_01_07_425794 162 19 . . . 10_1101-2021_01_07_425794 163 1 Figure figure NN 10_1101-2021_01_07_425794 163 2 3 3 CD 10_1101-2021_01_07_425794 163 3 shows show VBZ 10_1101-2021_01_07_425794 163 4 that that IN 10_1101-2021_01_07_425794 163 5 the the DT 10_1101-2021_01_07_425794 163 6 two two CD 10_1101-2021_01_07_425794 163 7 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 163 8 annotations annotation NNS 10_1101-2021_01_07_425794 163 9 exhibit exhibit VBP 10_1101-2021_01_07_425794 163 10 a a DT 10_1101-2021_01_07_425794 163 11 desirable desirable JJ 10_1101-2021_01_07_425794 163 12 larger large JJR 10_1101-2021_01_07_425794 163 13 intensity intensity NN 10_1101-2021_01_07_425794 163 14 range range NN 10_1101-2021_01_07_425794 163 15 of of IN 10_1101-2021_01_07_425794 163 16 gene gene NN 10_1101-2021_01_07_425794 163 17 expression expression NN 10_1101-2021_01_07_425794 163 18 than than IN 10_1101-2021_01_07_425794 163 19 the the DT 10_1101-2021_01_07_425794 163 20 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 163 21 annotation annotation NN 10_1101-2021_01_07_425794 163 22 , , , 10_1101-2021_01_07_425794 163 23 as as IN 10_1101-2021_01_07_425794 163 24 shown show VBN 10_1101-2021_01_07_425794 163 25 by by IN 10_1101-2021_01_07_425794 163 26 the the DT 10_1101-2021_01_07_425794 163 27 larger large JJR 10_1101-2021_01_07_425794 163 28 boxes box NNS 10_1101-2021_01_07_425794 163 29 in in IN 10_1101-2021_01_07_425794 163 30 the the DT 10_1101-2021_01_07_425794 163 31 boxplots boxplot NNS 10_1101-2021_01_07_425794 163 32 . . . 10_1101-2021_01_07_425794 164 1 It -PRON- PRP 10_1101-2021_01_07_425794 164 2 is be VBZ 10_1101-2021_01_07_425794 164 3 surprising surprising JJ 10_1101-2021_01_07_425794 164 4 to to TO 10_1101-2021_01_07_425794 164 5 see see VB 10_1101-2021_01_07_425794 164 6 that that IN 10_1101-2021_01_07_425794 164 7 the the DT 10_1101-2021_01_07_425794 164 8 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 164 9 genes gene NNS 10_1101-2021_01_07_425794 164 10 have have VBP 10_1101-2021_01_07_425794 164 11 the the DT 10_1101-2021_01_07_425794 164 12 smallest small JJS 10_1101-2021_01_07_425794 164 13 intensity intensity NN 10_1101-2021_01_07_425794 164 14 ranges range VBZ 10_1101-2021_01_07_425794 164 15 in in IN 10_1101-2021_01_07_425794 164 16 all all PDT 10_1101-2021_01_07_425794 164 17 the the DT 10_1101-2021_01_07_425794 164 18 libraries library NNS 10_1101-2021_01_07_425794 164 19 , , , 10_1101-2021_01_07_425794 164 20 give give VBP 10_1101-2021_01_07_425794 164 21 that that IN 10_1101-2021_01_07_425794 164 22 the the DT 10_1101-2021_01_07_425794 164 23 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 164 24 annotation annotation NN 10_1101-2021_01_07_425794 164 25 contains contain VBZ 10_1101-2021_01_07_425794 164 26 the the DT 10_1101-2021_01_07_425794 164 27 largest large JJS 10_1101-2021_01_07_425794 164 28 number number NN 10_1101-2021_01_07_425794 164 29 of of IN 10_1101-2021_01_07_425794 164 30 genes gene NNS 10_1101-2021_01_07_425794 164 31 in in IN 10_1101-2021_01_07_425794 164 32 all all PDT 10_1101-2021_01_07_425794 164 33 the the DT 10_1101-2021_01_07_425794 164 34 three three CD 10_1101-2021_01_07_425794 164 35 annotations annotation NNS 10_1101-2021_01_07_425794 164 36 being be VBG 10_1101-2021_01_07_425794 164 37 examined examine VBN 10_1101-2021_01_07_425794 164 38 . . . 10_1101-2021_01_07_425794 165 1 In in IN 10_1101-2021_01_07_425794 165 2 addition addition NN 10_1101-2021_01_07_425794 165 3 to to IN 10_1101-2021_01_07_425794 165 4 the the DT 10_1101-2021_01_07_425794 165 5 large large JJ 10_1101-2021_01_07_425794 165 6 intensity intensity NN 10_1101-2021_01_07_425794 165 7 range range NN 10_1101-2021_01_07_425794 165 8 , , , 10_1101-2021_01_07_425794 165 9 the the DT 10_1101-2021_01_07_425794 165 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 165 11 - - HYPH 10_1101-2021_01_07_425794 165 12 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 165 13 genes gene NNS 10_1101-2021_01_07_425794 165 14 were be VBD 10_1101-2021_01_07_425794 165 15 also also RB 10_1101-2021_01_07_425794 165 16 found find VBN 10_1101-2021_01_07_425794 165 17 to to TO 10_1101-2021_01_07_425794 165 18 have have VB 10_1101-2021_01_07_425794 165 19 a a DT 10_1101-2021_01_07_425794 165 20 markedly markedly RB 10_1101-2021_01_07_425794 165 21 higher high JJR 10_1101-2021_01_07_425794 165 22 median median JJ 10_1101-2021_01_07_425794 165 23 expression expression NN 10_1101-2021_01_07_425794 165 24 level level NN 10_1101-2021_01_07_425794 165 25 than than IN 10_1101-2021_01_07_425794 165 26 genes gene NNS 10_1101-2021_01_07_425794 165 27 in in IN 10_1101-2021_01_07_425794 165 28 the the DT 10_1101-2021_01_07_425794 165 29 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 165 30 - - HYPH 10_1101-2021_01_07_425794 165 31 NCBI NCBI NNP 10_1101-2021_01_07_425794 165 32 and and CC 10_1101-2021_01_07_425794 165 33 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 165 34 annotations annotation NNS 10_1101-2021_01_07_425794 165 35 . . . 10_1101-2021_01_07_425794 166 1 11 11 CD 10_1101-2021_01_07_425794 166 2 .CC .CC : 10_1101-2021_01_07_425794 166 3 - - HYPH 10_1101-2021_01_07_425794 166 4 BY by IN 10_1101-2021_01_07_425794 166 5 - - HYPH 10_1101-2021_01_07_425794 166 6 NC NC NNP 10_1101-2021_01_07_425794 166 7 - - HYPH 10_1101-2021_01_07_425794 166 8 ND ND NNP 10_1101-2021_01_07_425794 166 9 4.0 4.0 CD 10_1101-2021_01_07_425794 166 10 International international JJ 10_1101-2021_01_07_425794 166 11 licensemade licensemade NN 10_1101-2021_01_07_425794 166 12 available available JJ 10_1101-2021_01_07_425794 166 13 under under IN 10_1101-2021_01_07_425794 166 14 a a DT 10_1101-2021_01_07_425794 166 15 ( ( -LRB- 10_1101-2021_01_07_425794 166 16 which which WDT 10_1101-2021_01_07_425794 166 17 was be VBD 10_1101-2021_01_07_425794 166 18 not not RB 10_1101-2021_01_07_425794 166 19 certified certify VBN 10_1101-2021_01_07_425794 166 20 by by IN 10_1101-2021_01_07_425794 166 21 peer peer NN 10_1101-2021_01_07_425794 166 22 review review NN 10_1101-2021_01_07_425794 166 23 ) ) -RRB- 10_1101-2021_01_07_425794 166 24 is be VBZ 10_1101-2021_01_07_425794 166 25 the the DT 10_1101-2021_01_07_425794 166 26 author author NN 10_1101-2021_01_07_425794 166 27 / / SYM 10_1101-2021_01_07_425794 166 28 funder funder NN 10_1101-2021_01_07_425794 166 29 , , , 10_1101-2021_01_07_425794 166 30 who who WP 10_1101-2021_01_07_425794 166 31 has have VBZ 10_1101-2021_01_07_425794 166 32 granted grant VBN 10_1101-2021_01_07_425794 166 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 166 34 a a DT 10_1101-2021_01_07_425794 166 35 license license NN 10_1101-2021_01_07_425794 166 36 to to TO 10_1101-2021_01_07_425794 166 37 display display VB 10_1101-2021_01_07_425794 166 38 the the DT 10_1101-2021_01_07_425794 166 39 preprint preprint NN 10_1101-2021_01_07_425794 166 40 in in IN 10_1101-2021_01_07_425794 166 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 166 42 . . . 10_1101-2021_01_07_425794 167 1 It -PRON- PRP 10_1101-2021_01_07_425794 167 2 is be VBZ 10_1101-2021_01_07_425794 167 3 The the DT 10_1101-2021_01_07_425794 167 4 copyright copyright NN 10_1101-2021_01_07_425794 167 5 holder holder NN 10_1101-2021_01_07_425794 167 6 for for IN 10_1101-2021_01_07_425794 167 7 this this DT 10_1101-2021_01_07_425794 167 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 167 9 version version NN 10_1101-2021_01_07_425794 167 10 posted post VBD 10_1101-2021_01_07_425794 167 11 January January NNP 10_1101-2021_01_07_425794 167 12 8 8 CD 10_1101-2021_01_07_425794 167 13 , , , 10_1101-2021_01_07_425794 167 14 2021 2021 CD 10_1101-2021_01_07_425794 167 15 . . . 10_1101-2021_01_07_425794 167 16 ; ; : 10_1101-2021_01_07_425794 167 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 167 18 : : : 10_1101-2021_01_07_425794 167 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 167 20 preprint preprint JJ 10_1101-2021_01_07_425794 167 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 167 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 167 23 3.4 3.4 CD 10_1101-2021_01_07_425794 167 24 Gene gene NN 10_1101-2021_01_07_425794 167 25 annotation annotation NN 10_1101-2021_01_07_425794 167 26 discrepancy discrepancy NN 10_1101-2021_01_07_425794 167 27 after after IN 10_1101-2021_01_07_425794 167 28 expression expression NN 10_1101-2021_01_07_425794 167 29 filtering filter VBG 10_1101-2021_01_07_425794 167 30 As as IN 10_1101-2021_01_07_425794 167 31 it -PRON- PRP 10_1101-2021_01_07_425794 167 32 is be VBZ 10_1101-2021_01_07_425794 167 33 a a DT 10_1101-2021_01_07_425794 167 34 common common JJ 10_1101-2021_01_07_425794 167 35 practice practice NN 10_1101-2021_01_07_425794 167 36 to to TO 10_1101-2021_01_07_425794 167 37 filter filter VB 10_1101-2021_01_07_425794 167 38 out out RP 10_1101-2021_01_07_425794 167 39 genes gene NNS 10_1101-2021_01_07_425794 167 40 that that WDT 10_1101-2021_01_07_425794 167 41 are be VBP 10_1101-2021_01_07_425794 167 42 deemed deem VBN 10_1101-2021_01_07_425794 167 43 as as IN 10_1101-2021_01_07_425794 167 44 lowly lowly RB 10_1101-2021_01_07_425794 167 45 expressed express VBN 10_1101-2021_01_07_425794 167 46 , , , 10_1101-2021_01_07_425794 167 47 or or CC 10_1101-2021_01_07_425794 167 48 are be VBP 10_1101-2021_01_07_425794 167 49 completely completely RB 10_1101-2021_01_07_425794 167 50 absent absent JJ 10_1101-2021_01_07_425794 167 51 in in IN 10_1101-2021_01_07_425794 167 52 an an DT 10_1101-2021_01_07_425794 167 53 RNA RNA NNP 10_1101-2021_01_07_425794 167 54 - - HYPH 10_1101-2021_01_07_425794 167 55 seq seq NN 10_1101-2021_01_07_425794 167 56 data data NN 10_1101-2021_01_07_425794 167 57 analysis analysis NN 10_1101-2021_01_07_425794 167 58 [ [ -LRB- 10_1101-2021_01_07_425794 167 59 2 2 CD 10_1101-2021_01_07_425794 167 60 ] ] -RRB- 10_1101-2021_01_07_425794 167 61 , , , 10_1101-2021_01_07_425794 167 62 we -PRON- PRP 10_1101-2021_01_07_425794 167 63 also also RB 10_1101-2021_01_07_425794 167 64 set set VBD 10_1101-2021_01_07_425794 167 65 out out RP 10_1101-2021_01_07_425794 167 66 to to TO 10_1101-2021_01_07_425794 167 67 assess assess VB 10_1101-2021_01_07_425794 167 68 the the DT 10_1101-2021_01_07_425794 167 69 differences difference NNS 10_1101-2021_01_07_425794 167 70 between between IN 10_1101-2021_01_07_425794 167 71 alternative alternative JJ 10_1101-2021_01_07_425794 167 72 annotations annotation NNS 10_1101-2021_01_07_425794 167 73 after after IN 10_1101-2021_01_07_425794 167 74 excluding exclude VBG 10_1101-2021_01_07_425794 167 75 such such JJ 10_1101-2021_01_07_425794 167 76 genes gene NNS 10_1101-2021_01_07_425794 167 77 . . . 10_1101-2021_01_07_425794 168 1 We -PRON- PRP 10_1101-2021_01_07_425794 168 2 excluded exclude VBD 10_1101-2021_01_07_425794 168 3 those those DT 10_1101-2021_01_07_425794 168 4 genes gene NNS 10_1101-2021_01_07_425794 168 5 that that WDT 10_1101-2021_01_07_425794 168 6 failed fail VBD 10_1101-2021_01_07_425794 168 7 to to TO 10_1101-2021_01_07_425794 168 8 have have VB 10_1101-2021_01_07_425794 168 9 at at RB 10_1101-2021_01_07_425794 168 10 least least RBS 10_1101-2021_01_07_425794 168 11 0.5 0.5 CD 10_1101-2021_01_07_425794 168 12 CPM cpm NN 10_1101-2021_01_07_425794 168 13 ( ( -LRB- 10_1101-2021_01_07_425794 168 14 counts count NNS 10_1101-2021_01_07_425794 168 15 per per IN 10_1101-2021_01_07_425794 168 16 million million CD 10_1101-2021_01_07_425794 168 17 ) ) -RRB- 10_1101-2021_01_07_425794 168 18 in in IN 10_1101-2021_01_07_425794 168 19 at at RB 10_1101-2021_01_07_425794 168 20 least least RBS 10_1101-2021_01_07_425794 168 21 four four CD 10_1101-2021_01_07_425794 168 22 libraries library NNS 10_1101-2021_01_07_425794 168 23 ( ( -LRB- 10_1101-2021_01_07_425794 168 24 each each DT 10_1101-2021_01_07_425794 168 25 sample sample NN 10_1101-2021_01_07_425794 168 26 has have VBZ 10_1101-2021_01_07_425794 168 27 four four CD 10_1101-2021_01_07_425794 168 28 replicates replicate NNS 10_1101-2021_01_07_425794 168 29 ) ) -RRB- 10_1101-2021_01_07_425794 168 30 in in IN 10_1101-2021_01_07_425794 168 31 the the DT 10_1101-2021_01_07_425794 168 32 analysis analysis NN 10_1101-2021_01_07_425794 168 33 of of IN 10_1101-2021_01_07_425794 168 34 the the DT 10_1101-2021_01_07_425794 168 35 SEQC SEQC NNP 10_1101-2021_01_07_425794 168 36 dataset dataset NN 10_1101-2021_01_07_425794 168 37 . . . 10_1101-2021_01_07_425794 169 1 The the DT 10_1101-2021_01_07_425794 169 2 expression expression NN 10_1101-2021_01_07_425794 169 3 - - HYPH 10_1101-2021_01_07_425794 169 4 filtered filter VBN 10_1101-2021_01_07_425794 169 5 data datum NNS 10_1101-2021_01_07_425794 169 6 were be VBD 10_1101-2021_01_07_425794 169 7 also also RB 10_1101-2021_01_07_425794 169 8 used use VBN 10_1101-2021_01_07_425794 169 9 for for IN 10_1101-2021_01_07_425794 169 10 comparing compare VBG 10_1101-2021_01_07_425794 169 11 the the DT 10_1101-2021_01_07_425794 169 12 accuracy accuracy NN 10_1101-2021_01_07_425794 169 13 of of IN 10_1101-2021_01_07_425794 169 14 quantification quantification NN 10_1101-2021_01_07_425794 169 15 from from IN 10_1101-2021_01_07_425794 169 16 using use VBG 10_1101-2021_01_07_425794 169 17 alternative alternative JJ 10_1101-2021_01_07_425794 169 18 annotations annotation NNS 10_1101-2021_01_07_425794 169 19 presented present VBN 10_1101-2021_01_07_425794 169 20 in in IN 10_1101-2021_01_07_425794 169 21 the the DT 10_1101-2021_01_07_425794 169 22 following following JJ 10_1101-2021_01_07_425794 169 23 sections section NNS 10_1101-2021_01_07_425794 169 24 . . . 10_1101-2021_01_07_425794 170 1 The the DT 10_1101-2021_01_07_425794 170 2 bar bar NN 10_1101-2021_01_07_425794 170 3 plot plot NN 10_1101-2021_01_07_425794 170 4 in in IN 10_1101-2021_01_07_425794 170 5 Figure figure NN 10_1101-2021_01_07_425794 170 6 4A 4a NN 10_1101-2021_01_07_425794 170 7 shows show VBZ 10_1101-2021_01_07_425794 170 8 that that IN 10_1101-2021_01_07_425794 170 9 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 170 10 has have VBZ 10_1101-2021_01_07_425794 170 11 significantly significantly RB 10_1101-2021_01_07_425794 170 12 more more JJR 10_1101-2021_01_07_425794 170 13 genes gene NNS 10_1101-2021_01_07_425794 170 14 ( ( -LRB- 10_1101-2021_01_07_425794 170 15 also also RB 10_1101-2021_01_07_425794 170 16 higher high JJR 10_1101-2021_01_07_425794 170 17 proportion proportion NN 10_1101-2021_01_07_425794 170 18 of of IN 10_1101-2021_01_07_425794 170 19 genes gene NNS 10_1101-2021_01_07_425794 170 20 ) ) -RRB- 10_1101-2021_01_07_425794 170 21 filtered filter VBD 10_1101-2021_01_07_425794 170 22 out out RP 10_1101-2021_01_07_425794 170 23 due due IN 10_1101-2021_01_07_425794 170 24 to to IN 10_1101-2021_01_07_425794 170 25 low low JJ 10_1101-2021_01_07_425794 170 26 or or CC 10_1101-2021_01_07_425794 170 27 no no DT 10_1101-2021_01_07_425794 170 28 expression expression NN 10_1101-2021_01_07_425794 170 29 , , , 10_1101-2021_01_07_425794 170 30 compared compare VBN 10_1101-2021_01_07_425794 170 31 to to IN 10_1101-2021_01_07_425794 170 32 RefSeq- RefSeq- NNP 10_1101-2021_01_07_425794 170 33 NCBI NCBI NNP 10_1101-2021_01_07_425794 170 34 and and CC 10_1101-2021_01_07_425794 170 35 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 170 36 - - HYPH 10_1101-2021_01_07_425794 170 37 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 170 38 . . . 10_1101-2021_01_07_425794 171 1 After after IN 10_1101-2021_01_07_425794 171 2 expression expression NN 10_1101-2021_01_07_425794 171 3 filtering filtering NN 10_1101-2021_01_07_425794 171 4 , , , 10_1101-2021_01_07_425794 171 5 the the DT 10_1101-2021_01_07_425794 171 6 total total JJ 10_1101-2021_01_07_425794 171 7 numbers number NNS 10_1101-2021_01_07_425794 171 8 of of IN 10_1101-2021_01_07_425794 171 9 remaining remain VBG 10_1101-2021_01_07_425794 171 10 genes gene NNS 10_1101-2021_01_07_425794 171 11 from from IN 10_1101-2021_01_07_425794 171 12 the the DT 10_1101-2021_01_07_425794 171 13 three three CD 10_1101-2021_01_07_425794 171 14 annotations annotation NNS 10_1101-2021_01_07_425794 171 15 became become VBD 10_1101-2021_01_07_425794 171 16 more more RBR 10_1101-2021_01_07_425794 171 17 similar similar JJ 10_1101-2021_01_07_425794 171 18 to to IN 10_1101-2021_01_07_425794 171 19 each each DT 10_1101-2021_01_07_425794 171 20 other other JJ 10_1101-2021_01_07_425794 171 21 . . . 10_1101-2021_01_07_425794 172 1 16,472 16,472 CD 10_1101-2021_01_07_425794 172 2 genes gene NNS 10_1101-2021_01_07_425794 172 3 were be VBD 10_1101-2021_01_07_425794 172 4 found find VBN 10_1101-2021_01_07_425794 172 5 to to TO 10_1101-2021_01_07_425794 172 6 be be VB 10_1101-2021_01_07_425794 172 7 common common JJ 10_1101-2021_01_07_425794 172 8 between between IN 10_1101-2021_01_07_425794 172 9 the the DT 10_1101-2021_01_07_425794 172 10 three three CD 10_1101-2021_01_07_425794 172 11 annotations annotation NNS 10_1101-2021_01_07_425794 172 12 after after IN 10_1101-2021_01_07_425794 172 13 filtering filter VBG 10_1101-2021_01_07_425794 172 14 , , , 10_1101-2021_01_07_425794 172 15 accounting account VBG 10_1101-2021_01_07_425794 172 16 for for IN 10_1101-2021_01_07_425794 172 17 69 69 CD 10_1101-2021_01_07_425794 172 18 % % NN 10_1101-2021_01_07_425794 172 19 , , , 10_1101-2021_01_07_425794 172 20 78 78 CD 10_1101-2021_01_07_425794 172 21 % % NN 10_1101-2021_01_07_425794 172 22 and and CC 10_1101-2021_01_07_425794 172 23 86 86 CD 10_1101-2021_01_07_425794 172 24 % % NN 10_1101-2021_01_07_425794 172 25 of of IN 10_1101-2021_01_07_425794 172 26 the the DT 10_1101-2021_01_07_425794 172 27 filtered filter VBN 10_1101-2021_01_07_425794 172 28 genes gene NNS 10_1101-2021_01_07_425794 172 29 in in IN 10_1101-2021_01_07_425794 172 30 the the DT 10_1101-2021_01_07_425794 172 31 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 172 32 , , , 10_1101-2021_01_07_425794 172 33 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 172 34 - - HYPH 10_1101-2021_01_07_425794 172 35 NCBI NCBI NNP 10_1101-2021_01_07_425794 172 36 and and CC 10_1101-2021_01_07_425794 172 37 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 172 38 - - HYPH 10_1101-2021_01_07_425794 172 39 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 172 40 an- an- CC 10_1101-2021_01_07_425794 172 41 notations notation NNS 10_1101-2021_01_07_425794 172 42 respectively respectively RB 10_1101-2021_01_07_425794 172 43 ( ( -LRB- 10_1101-2021_01_07_425794 172 44 Figure figure NN 10_1101-2021_01_07_425794 172 45 4B 4b NN 10_1101-2021_01_07_425794 172 46 ) ) -RRB- 10_1101-2021_01_07_425794 172 47 . . . 10_1101-2021_01_07_425794 173 1 Almost almost RB 10_1101-2021_01_07_425794 173 2 all all PDT 10_1101-2021_01_07_425794 173 3 the the DT 10_1101-2021_01_07_425794 173 4 filtered filter VBN 10_1101-2021_01_07_425794 173 5 genes gene NNS 10_1101-2021_01_07_425794 173 6 in in IN 10_1101-2021_01_07_425794 173 7 the the DT 10_1101-2021_01_07_425794 173 8 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 173 9 - - HYPH 10_1101-2021_01_07_425794 173 10 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 173 11 annotation annotation NN 10_1101-2021_01_07_425794 173 12 can can MD 10_1101-2021_01_07_425794 173 13 be be VB 10_1101-2021_01_07_425794 173 14 found find VBN 10_1101-2021_01_07_425794 173 15 in in IN 10_1101-2021_01_07_425794 173 16 the the DT 10_1101-2021_01_07_425794 173 17 other other JJ 10_1101-2021_01_07_425794 173 18 two two CD 10_1101-2021_01_07_425794 173 19 annotations annotation NNS 10_1101-2021_01_07_425794 173 20 . . . 10_1101-2021_01_07_425794 174 1 After after IN 10_1101-2021_01_07_425794 174 2 expression expression NN 10_1101-2021_01_07_425794 174 3 filtering filtering NN 10_1101-2021_01_07_425794 174 4 , , , 10_1101-2021_01_07_425794 174 5 the the DT 10_1101-2021_01_07_425794 174 6 median median JJ 10_1101-2021_01_07_425794 174 7 effective effective JJ 10_1101-2021_01_07_425794 174 8 gene gene NN 10_1101-2021_01_07_425794 174 9 length length NN 10_1101-2021_01_07_425794 174 10 has have VBZ 10_1101-2021_01_07_425794 174 11 increased increase VBN 10_1101-2021_01_07_425794 174 12 to to IN 10_1101-2021_01_07_425794 174 13 ∼4,000 ∼4,000 CD 10_1101-2021_01_07_425794 174 14 bases basis NNS 10_1101-2021_01_07_425794 174 15 for for IN 10_1101-2021_01_07_425794 174 16 all all DT 10_1101-2021_01_07_425794 174 17 annotations annotation NNS 10_1101-2021_01_07_425794 174 18 ( ( -LRB- 10_1101-2021_01_07_425794 174 19 Figure figure NN 10_1101-2021_01_07_425794 174 20 4C 4c NN 10_1101-2021_01_07_425794 174 21 ) ) -RRB- 10_1101-2021_01_07_425794 174 22 , , , 10_1101-2021_01_07_425794 174 23 meaning mean VBG 10_1101-2021_01_07_425794 174 24 that that IN 10_1101-2021_01_07_425794 174 25 a a DT 10_1101-2021_01_07_425794 174 26 higher high JJR 10_1101-2021_01_07_425794 174 27 proportion proportion NN 10_1101-2021_01_07_425794 174 28 of of IN 10_1101-2021_01_07_425794 174 29 short short JJ 10_1101-2021_01_07_425794 174 30 genes gene NNS 10_1101-2021_01_07_425794 174 31 were be VBD 10_1101-2021_01_07_425794 174 32 removed remove VBN 10_1101-2021_01_07_425794 174 33 due due IN 10_1101-2021_01_07_425794 174 34 to to IN 10_1101-2021_01_07_425794 174 35 low low JJ 10_1101-2021_01_07_425794 174 36 expression expression NN 10_1101-2021_01_07_425794 174 37 in in IN 10_1101-2021_01_07_425794 174 38 every every DT 10_1101-2021_01_07_425794 174 39 annotation annotation NN 10_1101-2021_01_07_425794 174 40 . . . 10_1101-2021_01_07_425794 175 1 The the DT 10_1101-2021_01_07_425794 175 2 median median JJ 10_1101-2021_01_07_425794 175 3 effective effective JJ 10_1101-2021_01_07_425794 175 4 length length NN 10_1101-2021_01_07_425794 175 5 of of IN 10_1101-2021_01_07_425794 175 6 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 175 7 genes gene NNS 10_1101-2021_01_07_425794 175 8 now now RB 10_1101-2021_01_07_425794 175 9 became become VBD 10_1101-2021_01_07_425794 175 10 comparable comparable JJ 10_1101-2021_01_07_425794 175 11 to to IN 10_1101-2021_01_07_425794 175 12 , , , 10_1101-2021_01_07_425794 175 13 or or CC 10_1101-2021_01_07_425794 175 14 slightly slightly RB 10_1101-2021_01_07_425794 175 15 higher high JJR 10_1101-2021_01_07_425794 175 16 than than IN 10_1101-2021_01_07_425794 175 17 those those DT 10_1101-2021_01_07_425794 175 18 in in IN 10_1101-2021_01_07_425794 175 19 the the DT 10_1101-2021_01_07_425794 175 20 two two CD 10_1101-2021_01_07_425794 175 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 175 22 annotations annotation NNS 10_1101-2021_01_07_425794 175 23 , , , 10_1101-2021_01_07_425794 175 24 indicating indicate VBG 10_1101-2021_01_07_425794 175 25 that that IN 10_1101-2021_01_07_425794 175 26 the the DT 10_1101-2021_01_07_425794 175 27 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 175 28 annotation annotation NN 10_1101-2021_01_07_425794 175 29 contained contain VBD 10_1101-2021_01_07_425794 175 30 a a DT 10_1101-2021_01_07_425794 175 31 higher high JJR 10_1101-2021_01_07_425794 175 32 proportion proportion NN 10_1101-2021_01_07_425794 175 33 of of IN 10_1101-2021_01_07_425794 175 34 lowly lowly JJ 10_1101-2021_01_07_425794 175 35 expressed express VBN 10_1101-2021_01_07_425794 175 36 short short JJ 10_1101-2021_01_07_425794 175 37 genes gene NNS 10_1101-2021_01_07_425794 175 38 than than IN 10_1101-2021_01_07_425794 175 39 the the DT 10_1101-2021_01_07_425794 175 40 two two CD 10_1101-2021_01_07_425794 175 41 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 175 42 annotations annotation NNS 10_1101-2021_01_07_425794 175 43 . . . 10_1101-2021_01_07_425794 176 1 When when WRB 10_1101-2021_01_07_425794 176 2 comparing compare VBG 10_1101-2021_01_07_425794 176 3 the the DT 10_1101-2021_01_07_425794 176 4 effective effective JJ 10_1101-2021_01_07_425794 176 5 lengths length NNS 10_1101-2021_01_07_425794 176 6 of of IN 10_1101-2021_01_07_425794 176 7 genes gene NNS 10_1101-2021_01_07_425794 176 8 common common JJ 10_1101-2021_01_07_425794 176 9 to to IN 10_1101-2021_01_07_425794 176 10 all all DT 10_1101-2021_01_07_425794 176 11 three three CD 10_1101-2021_01_07_425794 176 12 annotations annotation NNS 10_1101-2021_01_07_425794 176 13 after after IN 10_1101-2021_01_07_425794 176 14 filtering filter VBG 10_1101-2021_01_07_425794 176 15 , , , 10_1101-2021_01_07_425794 176 16 the the DT 10_1101-2021_01_07_425794 176 17 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 176 18 genes gene NNS 10_1101-2021_01_07_425794 176 19 were be VBD 10_1101-2021_01_07_425794 176 20 found find VBN 10_1101-2021_01_07_425794 176 21 to to TO 10_1101-2021_01_07_425794 176 22 have have VB 10_1101-2021_01_07_425794 176 23 the the DT 10_1101-2021_01_07_425794 176 24 largest large JJS 10_1101-2021_01_07_425794 176 25 median median JJ 10_1101-2021_01_07_425794 176 26 effective effective JJ 10_1101-2021_01_07_425794 176 27 length length NN 10_1101-2021_01_07_425794 176 28 and and CC 10_1101-2021_01_07_425794 176 29 the the DT 10_1101-2021_01_07_425794 176 30 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 176 31 - - HYPH 10_1101-2021_01_07_425794 176 32 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 176 33 genes gene NNS 10_1101-2021_01_07_425794 176 34 have have VBP 10_1101-2021_01_07_425794 176 35 the the DT 10_1101-2021_01_07_425794 176 36 smallest small JJS 10_1101-2021_01_07_425794 176 37 median median JJ 10_1101-2021_01_07_425794 176 38 effective effective JJ 10_1101-2021_01_07_425794 176 39 length length NN 10_1101-2021_01_07_425794 176 40 ( ( -LRB- 10_1101-2021_01_07_425794 176 41 Figure Figure NNP 10_1101-2021_01_07_425794 176 42 4D 4d NN 10_1101-2021_01_07_425794 176 43 ) ) -RRB- 10_1101-2021_01_07_425794 176 44 . . . 10_1101-2021_01_07_425794 177 1 This this DT 10_1101-2021_01_07_425794 177 2 is be VBZ 10_1101-2021_01_07_425794 177 3 not not RB 10_1101-2021_01_07_425794 177 4 surprising surprising JJ 10_1101-2021_01_07_425794 177 5 because because IN 10_1101-2021_01_07_425794 177 6 the the DT 10_1101-2021_01_07_425794 177 7 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 177 8 annotation annotation NN 10_1101-2021_01_07_425794 177 9 is be VBZ 10_1101-2021_01_07_425794 177 10 known know VBN 10_1101-2021_01_07_425794 177 11 to to TO 10_1101-2021_01_07_425794 177 12 be be VB 10_1101-2021_01_07_425794 177 13 more more RBR 10_1101-2021_01_07_425794 177 14 aggressive aggressive JJ 10_1101-2021_01_07_425794 177 15 than than IN 10_1101-2021_01_07_425794 177 16 the the DT 10_1101-2021_01_07_425794 177 17 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 177 18 annotations annotation NNS 10_1101-2021_01_07_425794 177 19 and and CC 10_1101-2021_01_07_425794 177 20 the the DT 10_1101-2021_01_07_425794 177 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 177 22 - - HYPH 10_1101-2021_01_07_425794 177 23 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 177 24 annotation annotation NN 10_1101-2021_01_07_425794 177 25 is be VBZ 10_1101-2021_01_07_425794 177 26 an an DT 10_1101-2021_01_07_425794 177 27 old old JJ 10_1101-2021_01_07_425794 177 28 annotation annotation NN 10_1101-2021_01_07_425794 177 29 that that WDT 10_1101-2021_01_07_425794 177 30 has have VBZ 10_1101-2021_01_07_425794 177 31 not not RB 10_1101-2021_01_07_425794 177 32 been be VBN 10_1101-2021_01_07_425794 177 33 updated update VBN 10_1101-2021_01_07_425794 177 34 in in IN 10_1101-2021_01_07_425794 177 35 the the DT 10_1101-2021_01_07_425794 177 36 last last JJ 10_1101-2021_01_07_425794 177 37 five five CD 10_1101-2021_01_07_425794 177 38 year year NN 10_1101-2021_01_07_425794 177 39 . . . 10_1101-2021_01_07_425794 178 1 The the DT 10_1101-2021_01_07_425794 178 2 expression expression NN 10_1101-2021_01_07_425794 178 3 filtering filtering NN 10_1101-2021_01_07_425794 178 4 did do VBD 10_1101-2021_01_07_425794 178 5 not not RB 10_1101-2021_01_07_425794 178 6 seem seem VB 10_1101-2021_01_07_425794 178 7 to to TO 10_1101-2021_01_07_425794 178 8 affect affect VB 10_1101-2021_01_07_425794 178 9 the the DT 10_1101-2021_01_07_425794 178 10 distribution distribution NN 10_1101-2021_01_07_425794 178 11 of of IN 10_1101-2021_01_07_425794 178 12 differences difference NNS 10_1101-2021_01_07_425794 178 13 of of IN 10_1101-2021_01_07_425794 178 14 effective effective JJ 10_1101-2021_01_07_425794 178 15 gene gene NN 10_1101-2021_01_07_425794 178 16 lengths length NNS 10_1101-2021_01_07_425794 178 17 between between IN 10_1101-2021_01_07_425794 178 18 each each DT 10_1101-2021_01_07_425794 178 19 pair pair NN 10_1101-2021_01_07_425794 178 20 of of IN 10_1101-2021_01_07_425794 178 21 annotations annotation NNS 10_1101-2021_01_07_425794 178 22 ( ( -LRB- 10_1101-2021_01_07_425794 178 23 using use VBG 10_1101-2021_01_07_425794 178 24 genes gene NNS 10_1101-2021_01_07_425794 178 25 common common JJ 10_1101-2021_01_07_425794 178 26 to to IN 10_1101-2021_01_07_425794 178 27 each each DT 10_1101-2021_01_07_425794 178 28 pair pair NN 10_1101-2021_01_07_425794 178 29 of of IN 10_1101-2021_01_07_425794 178 30 annotations annotation NNS 10_1101-2021_01_07_425794 178 31 ) ) -RRB- 10_1101-2021_01_07_425794 178 32 , , , 10_1101-2021_01_07_425794 178 33 with with IN 10_1101-2021_01_07_425794 178 34 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 178 35 and and CC 10_1101-2021_01_07_425794 178 36 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 178 37 - - HYPH 10_1101-2021_01_07_425794 178 38 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 178 39 remaining remain VBG 10_1101-2021_01_07_425794 178 40 to to TO 10_1101-2021_01_07_425794 178 41 be be VB 10_1101-2021_01_07_425794 178 42 the the DT 10_1101-2021_01_07_425794 178 43 least least JJS 10_1101-2021_01_07_425794 178 44 concordant concordant NN 10_1101-2021_01_07_425794 178 45 annotations annotation NNS 10_1101-2021_01_07_425794 178 46 ( ( -LRB- 10_1101-2021_01_07_425794 178 47 Figure figure NN 10_1101-2021_01_07_425794 178 48 4E 4e NN 10_1101-2021_01_07_425794 178 49 and and CC 10_1101-2021_01_07_425794 178 50 Figure figure NN 10_1101-2021_01_07_425794 178 51 1C 1c NN 10_1101-2021_01_07_425794 178 52 ) ) -RRB- 10_1101-2021_01_07_425794 178 53 . . . 10_1101-2021_01_07_425794 179 1 Using use VBG 10_1101-2021_01_07_425794 179 2 genes gene NNS 10_1101-2021_01_07_425794 179 3 common common JJ 10_1101-2021_01_07_425794 179 4 to to IN 10_1101-2021_01_07_425794 179 5 all all DT 10_1101-2021_01_07_425794 179 6 three three CD 10_1101-2021_01_07_425794 179 7 annotations annotation NNS 10_1101-2021_01_07_425794 179 8 after after IN 10_1101-2021_01_07_425794 179 9 filtering filter VBG 10_1101-2021_01_07_425794 179 10 exhibited exhibit VBD 10_1101-2021_01_07_425794 179 11 similar similar JJ 10_1101-2021_01_07_425794 179 12 distributions distribution NNS 10_1101-2021_01_07_425794 179 13 of of IN 10_1101-2021_01_07_425794 179 14 gene gene NN 10_1101-2021_01_07_425794 179 15 length length NN 10_1101-2021_01_07_425794 179 16 differences difference NNS 10_1101-2021_01_07_425794 179 17 between between IN 10_1101-2021_01_07_425794 179 18 each each DT 10_1101-2021_01_07_425794 179 19 pair pair NN 10_1101-2021_01_07_425794 179 20 of of IN 10_1101-2021_01_07_425794 179 21 annotations annotation NNS 10_1101-2021_01_07_425794 179 22 compared compare VBN 10_1101-2021_01_07_425794 179 23 to to IN 10_1101-2021_01_07_425794 179 24 using use VBG 10_1101-2021_01_07_425794 179 25 genes gene NNS 10_1101-2021_01_07_425794 179 26 common common JJ 10_1101-2021_01_07_425794 179 27 to to IN 10_1101-2021_01_07_425794 179 28 each each DT 10_1101-2021_01_07_425794 179 29 pair pair NN 10_1101-2021_01_07_425794 179 30 of of IN 10_1101-2021_01_07_425794 179 31 annotations annotation NNS 10_1101-2021_01_07_425794 179 32 ( ( -LRB- 10_1101-2021_01_07_425794 179 33 Figure Figure NNP 10_1101-2021_01_07_425794 179 34 4F 4f NN 10_1101-2021_01_07_425794 179 35 ) ) -RRB- 10_1101-2021_01_07_425794 179 36 . . . 10_1101-2021_01_07_425794 180 1 Similar similar JJ 10_1101-2021_01_07_425794 180 2 to to IN 10_1101-2021_01_07_425794 180 3 before before IN 10_1101-2021_01_07_425794 180 4 filtering filtering NN 10_1101-2021_01_07_425794 180 5 , , , 10_1101-2021_01_07_425794 180 6 the the DT 10_1101-2021_01_07_425794 180 7 gene gene NN 10_1101-2021_01_07_425794 180 8 - - HYPH 10_1101-2021_01_07_425794 180 9 wise wise JJ 10_1101-2021_01_07_425794 180 10 length length NN 10_1101-2021_01_07_425794 180 11 comparison comparison NN 10_1101-2021_01_07_425794 180 12 performed perform VBN 10_1101-2021_01_07_425794 180 13 after after IN 10_1101-2021_01_07_425794 180 14 filtering filter VBG 10_1101-2021_01_07_425794 180 15 also also RB 10_1101-2021_01_07_425794 180 16 showed show VBD 10_1101-2021_01_07_425794 180 17 that that IN 10_1101-2021_01_07_425794 180 18 overall overall RB 10_1101-2021_01_07_425794 180 19 the the DT 10_1101-2021_01_07_425794 180 20 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 180 21 genes gene NNS 10_1101-2021_01_07_425794 180 22 had have VBD 10_1101-2021_01_07_425794 180 23 the the DT 10_1101-2021_01_07_425794 180 24 largest large JJS 10_1101-2021_01_07_425794 180 25 gene gene NN 10_1101-2021_01_07_425794 180 26 lengths length NNS 10_1101-2021_01_07_425794 180 27 and and CC 10_1101-2021_01_07_425794 180 28 the the DT 10_1101-2021_01_07_425794 180 29 RefSeq- refseq- JJ 10_1101-2021_01_07_425794 180 30 12 12 CD 10_1101-2021_01_07_425794 180 31 .CC .CC , 10_1101-2021_01_07_425794 180 32 - - HYPH 10_1101-2021_01_07_425794 180 33 BY by IN 10_1101-2021_01_07_425794 180 34 - - HYPH 10_1101-2021_01_07_425794 180 35 NC NC NNP 10_1101-2021_01_07_425794 180 36 - - HYPH 10_1101-2021_01_07_425794 180 37 ND ND NNP 10_1101-2021_01_07_425794 180 38 4.0 4.0 CD 10_1101-2021_01_07_425794 180 39 International international JJ 10_1101-2021_01_07_425794 180 40 licensemade licensemade NN 10_1101-2021_01_07_425794 180 41 available available JJ 10_1101-2021_01_07_425794 180 42 under under IN 10_1101-2021_01_07_425794 180 43 a a DT 10_1101-2021_01_07_425794 180 44 ( ( -LRB- 10_1101-2021_01_07_425794 180 45 which which WDT 10_1101-2021_01_07_425794 180 46 was be VBD 10_1101-2021_01_07_425794 180 47 not not RB 10_1101-2021_01_07_425794 180 48 certified certify VBN 10_1101-2021_01_07_425794 180 49 by by IN 10_1101-2021_01_07_425794 180 50 peer peer NN 10_1101-2021_01_07_425794 180 51 review review NN 10_1101-2021_01_07_425794 180 52 ) ) -RRB- 10_1101-2021_01_07_425794 180 53 is be VBZ 10_1101-2021_01_07_425794 180 54 the the DT 10_1101-2021_01_07_425794 180 55 author author NN 10_1101-2021_01_07_425794 180 56 / / SYM 10_1101-2021_01_07_425794 180 57 funder funder NN 10_1101-2021_01_07_425794 180 58 , , , 10_1101-2021_01_07_425794 180 59 who who WP 10_1101-2021_01_07_425794 180 60 has have VBZ 10_1101-2021_01_07_425794 180 61 granted grant VBN 10_1101-2021_01_07_425794 180 62 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 180 63 a a DT 10_1101-2021_01_07_425794 180 64 license license NN 10_1101-2021_01_07_425794 180 65 to to TO 10_1101-2021_01_07_425794 180 66 display display VB 10_1101-2021_01_07_425794 180 67 the the DT 10_1101-2021_01_07_425794 180 68 preprint preprint NN 10_1101-2021_01_07_425794 180 69 in in IN 10_1101-2021_01_07_425794 180 70 perpetuity perpetuity NN 10_1101-2021_01_07_425794 180 71 . . . 10_1101-2021_01_07_425794 181 1 It -PRON- PRP 10_1101-2021_01_07_425794 181 2 is be VBZ 10_1101-2021_01_07_425794 181 3 The the DT 10_1101-2021_01_07_425794 181 4 copyright copyright NN 10_1101-2021_01_07_425794 181 5 holder holder NN 10_1101-2021_01_07_425794 181 6 for for IN 10_1101-2021_01_07_425794 181 7 this this DT 10_1101-2021_01_07_425794 181 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 181 9 version version NN 10_1101-2021_01_07_425794 181 10 posted post VBD 10_1101-2021_01_07_425794 181 11 January January NNP 10_1101-2021_01_07_425794 181 12 8 8 CD 10_1101-2021_01_07_425794 181 13 , , , 10_1101-2021_01_07_425794 181 14 2021 2021 CD 10_1101-2021_01_07_425794 181 15 . . . 10_1101-2021_01_07_425794 181 16 ; ; : 10_1101-2021_01_07_425794 181 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 181 18 : : : 10_1101-2021_01_07_425794 181 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 181 20 preprint preprint JJ 10_1101-2021_01_07_425794 181 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 181 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425794 181 23 1,996 1,996 CD 10_1101-2021_01_07_425794 181 24 376 376 CD 10_1101-2021_01_07_425794 181 25 2,141 2,141 CD 10_1101-2021_01_07_425794 181 26 6,837 6,837 CD 10_1101-2021_01_07_425794 181 27 489 489 CD 10_1101-2021_01_07_425794 181 28 71 71 CD 10_1101-2021_01_07_425794 181 29 16,472 16,472 CD 10_1101-2021_01_07_425794 181 30 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 181 31 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 181 32 - - HYPH 10_1101-2021_01_07_425794 181 33 NCBI NCBI NNP 10_1101-2021_01_07_425794 181 34 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 181 35 - - HYPH 10_1101-2021_01_07_425794 181 36 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 181 37 B B NNP 10_1101-2021_01_07_425794 181 38 D D NNP 10_1101-2021_01_07_425794 181 39 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 181 40 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 181 41 - - HYPH 10_1101-2021_01_07_425794 181 42 RsubreadRefSeq RsubreadRefSeq NNP 10_1101-2021_01_07_425794 181 43 - - HYPH 10_1101-2021_01_07_425794 181 44 NCBI NCBI NNP 10_1101-2021_01_07_425794 181 45 Before before IN 10_1101-2021_01_07_425794 181 46 After after IN 10_1101-2021_01_07_425794 181 47 G G NNP 10_1101-2021_01_07_425794 181 48 en en NNP 10_1101-2021_01_07_425794 181 49 e e NNP 10_1101-2021_01_07_425794 181 50 co co NN 10_1101-2021_01_07_425794 181 51 un un NNP 10_1101-2021_01_07_425794 181 52 t t NNP 10_1101-2021_01_07_425794 181 53 ( ( -LRB- 10_1101-2021_01_07_425794 181 54 x1 x1 NNP 10_1101-2021_01_07_425794 181 55 00 00 CD 10_1101-2021_01_07_425794 181 56 0 0 CD 10_1101-2021_01_07_425794 181 57 ) ) -RRB- 10_1101-2021_01_07_425794 181 58 10 10 CD 10_1101-2021_01_07_425794 181 59 30 30 CD 10_1101-2021_01_07_425794 181 60 50 50 CD 10_1101-2021_01_07_425794 181 61 60,683 60,683 CD 10_1101-2021_01_07_425794 181 62 23,869 23,869 CD 10_1101-2021_01_07_425794 181 63 28,395 28,395 CD 10_1101-2021_01_07_425794 181 64 19,060 19,060 CD 10_1101-2021_01_07_425794 181 65 39,535 39,535 CD 10_1101-2021_01_07_425794 181 66 21,098 21,098 CD 10_1101-2021_01_07_425794 181 67 A a NN 10_1101-2021_01_07_425794 181 68 E e NN 10_1101-2021_01_07_425794 181 69 ns ns IN 10_1101-2021_01_07_425794 181 70 em -PRON- PRP 10_1101-2021_01_07_425794 181 71 bl bl NNP 10_1101-2021_01_07_425794 181 72 R R NNP 10_1101-2021_01_07_425794 181 73 ef ef NNP 10_1101-2021_01_07_425794 181 74 S S NNP 10_1101-2021_01_07_425794 181 75 eq eq NNP 10_1101-2021_01_07_425794 181 76 -N -n NN 10_1101-2021_01_07_425794 181 77 C C NNP 10_1101-2021_01_07_425794 181 78 B B NNP 10_1101-2021_01_07_425794 181 79 I I NNP 10_1101-2021_01_07_425794 181 80 R r NN 10_1101-2021_01_07_425794 181 81 ef ef NNP 10_1101-2021_01_07_425794 181 82 S S NNP 10_1101-2021_01_07_425794 181 83 eq eq NNP 10_1101-2021_01_07_425794 181 84 -R -R NNP 10_1101-2021_01_07_425794 181 85 su su NNP 10_1101-2021_01_07_425794 181 86 br br VBP 10_1101-2021_01_07_425794 181 87 ea ea NNP 10_1101-2021_01_07_425794 181 88 d d NNP 10_1101-2021_01_07_425794 181 89 Lo Lo NNP 10_1101-2021_01_07_425794 181 90 g2 g2 NNP 10_1101-2021_01_07_425794 181 91 ef ef NNP 10_1101-2021_01_07_425794 181 92 fe fe NNP 10_1101-2021_01_07_425794 181 93 ct ct NNP 10_1101-2021_01_07_425794 181 94 iv iv NNP 10_1101-2021_01_07_425794 181 95 e e NNP 10_1101-2021_01_07_425794 181 96 ge ge NNP 10_1101-2021_01_07_425794 181 97 ne ne NNP 10_1101-2021_01_07_425794 181 98 le le NNP 10_1101-2021_01_07_425794 181 99 ng ng PRP 10_1101-2021_01_07_425794 181 100 th th XX 10_1101-2021_01_07_425794 181 101 D d NN 10_1101-2021_01_07_425794 181 102 iff iff NN 10_1101-2021_01_07_425794 181 103 er er UH 10_1101-2021_01_07_425794 181 104 en en FW 10_1101-2021_01_07_425794 181 105 ce ce NNP 10_1101-2021_01_07_425794 181 106 in in IN 10_1101-2021_01_07_425794 181 107 lo lo NNP 10_1101-2021_01_07_425794 181 108 g2 g2 NNP 10_1101-2021_01_07_425794 181 109 ef ef NNP 10_1101-2021_01_07_425794 181 110 fe fe NNP 10_1101-2021_01_07_425794 181 111 ct ct NNP 10_1101-2021_01_07_425794 181 112 iv iv NNP 10_1101-2021_01_07_425794 181 113 e e NNP 10_1101-2021_01_07_425794 181 114 ge ge NNP 10_1101-2021_01_07_425794 181 115 ne ne NNP 10_1101-2021_01_07_425794 181 116 le le NNP 10_1101-2021_01_07_425794 181 117 ng ng NNP 10_1101-2021_01_07_425794 181 118 th th XX 10_1101-2021_01_07_425794 181 119 F F NNP 10_1101-2021_01_07_425794 181 120 R R NNP 10_1101-2021_01_07_425794 181 121 ef ef NNP 10_1101-2021_01_07_425794 181 122 S S NNP 10_1101-2021_01_07_425794 181 123 eq eq NN 10_1101-2021_01_07_425794 181 124 −N −N NNP 10_1101-2021_01_07_425794 181 125 C C NNP 10_1101-2021_01_07_425794 181 126 B B NNP 10_1101-2021_01_07_425794 181 127 I I NNP 10_1101-2021_01_07_425794 181 128 vs vs IN 10_1101-2021_01_07_425794 181 129 R r NN 10_1101-2021_01_07_425794 181 130 ef ef NNP 10_1101-2021_01_07_425794 181 131 S S NNP 10_1101-2021_01_07_425794 181 132 eq eq NNP 10_1101-2021_01_07_425794 181 133 −R −R NNP 10_1101-2021_01_07_425794 181 134 su su NNP 10_1101-2021_01_07_425794 181 135 br br VBP 10_1101-2021_01_07_425794 181 136 ea ea NN 10_1101-2021_01_07_425794 181 137 d d NN 10_1101-2021_01_07_425794 181 138 E e NN 10_1101-2021_01_07_425794 181 139 ns ns IN 10_1101-2021_01_07_425794 181 140 em -PRON- PRP 10_1101-2021_01_07_425794 181 141 bl bl PRP 10_1101-2021_01_07_425794 181 142 vs vs IN 10_1101-2021_01_07_425794 181 143 R R NNP 10_1101-2021_01_07_425794 181 144 ef ef NNP 10_1101-2021_01_07_425794 181 145 S S NNP 10_1101-2021_01_07_425794 181 146 eq eq NNP 10_1101-2021_01_07_425794 181 147 −R −R NNP 10_1101-2021_01_07_425794 181 148 su su NNP 10_1101-2021_01_07_425794 181 149 br br VBP 10_1101-2021_01_07_425794 181 150 ea ea NN 10_1101-2021_01_07_425794 181 151 d d NN 10_1101-2021_01_07_425794 181 152 E e NN 10_1101-2021_01_07_425794 181 153 ns ns IN 10_1101-2021_01_07_425794 181 154 em -PRON- PRP 10_1101-2021_01_07_425794 181 155 bl bl PRP 10_1101-2021_01_07_425794 181 156 vs vs IN 10_1101-2021_01_07_425794 181 157 R R NNP 10_1101-2021_01_07_425794 181 158 ef ef NNP 10_1101-2021_01_07_425794 181 159 S S NNP 10_1101-2021_01_07_425794 181 160 eq eq NNP 10_1101-2021_01_07_425794 181 161 -N -n NN 10_1101-2021_01_07_425794 181 162 C C NNP 10_1101-2021_01_07_425794 181 163 B B NNP 10_1101-2021_01_07_425794 181 164 I i NN 10_1101-2021_01_07_425794 181 165 E e NN 10_1101-2021_01_07_425794 181 166 ns ns IN 10_1101-2021_01_07_425794 181 167 em -PRON- PRP 10_1101-2021_01_07_425794 181 168 bl bl NNP 10_1101-2021_01_07_425794 181 169 R R NNP 10_1101-2021_01_07_425794 181 170 ef ef NNP 10_1101-2021_01_07_425794 181 171 S S NNP 10_1101-2021_01_07_425794 181 172 eq eq NNP 10_1101-2021_01_07_425794 181 173 -N -n NN 10_1101-2021_01_07_425794 181 174 C C NNP 10_1101-2021_01_07_425794 181 175 B B NNP 10_1101-2021_01_07_425794 181 176 I I NNP 10_1101-2021_01_07_425794 181 177 R r NN 10_1101-2021_01_07_425794 181 178 ef ef NNP 10_1101-2021_01_07_425794 181 179 S S NNP 10_1101-2021_01_07_425794 181 180 eq eq NNP 10_1101-2021_01_07_425794 181 181 -R -R NNP 10_1101-2021_01_07_425794 181 182 su su NNP 10_1101-2021_01_07_425794 181 183 br br VBP 10_1101-2021_01_07_425794 181 184 ea ea NNP 10_1101-2021_01_07_425794 181 185 d d NN 10_1101-2021_01_07_425794 181 186 R r NN 10_1101-2021_01_07_425794 181 187 ef ef NN 10_1101-2021_01_07_425794 181 188 S S NNP 10_1101-2021_01_07_425794 181 189 eq eq NN 10_1101-2021_01_07_425794 181 190 −N −N NNP 10_1101-2021_01_07_425794 181 191 C C NNP 10_1101-2021_01_07_425794 181 192 B B NNP 10_1101-2021_01_07_425794 181 193 I I NNP 10_1101-2021_01_07_425794 181 194 vs vs IN 10_1101-2021_01_07_425794 181 195 R r NN 10_1101-2021_01_07_425794 181 196 ef ef NNP 10_1101-2021_01_07_425794 181 197 S S NNP 10_1101-2021_01_07_425794 181 198 eq eq NNP 10_1101-2021_01_07_425794 181 199 −R −R NNP 10_1101-2021_01_07_425794 181 200 su su NNP 10_1101-2021_01_07_425794 181 201 br br VBP 10_1101-2021_01_07_425794 181 202 ea ea NN 10_1101-2021_01_07_425794 181 203 d d NN 10_1101-2021_01_07_425794 181 204 E e NN 10_1101-2021_01_07_425794 181 205 ns ns IN 10_1101-2021_01_07_425794 181 206 em -PRON- PRP 10_1101-2021_01_07_425794 181 207 bl bl PRP 10_1101-2021_01_07_425794 181 208 vs vs IN 10_1101-2021_01_07_425794 181 209 R R NNP 10_1101-2021_01_07_425794 181 210 ef ef NNP 10_1101-2021_01_07_425794 181 211 S S NNP 10_1101-2021_01_07_425794 181 212 eq eq NNP 10_1101-2021_01_07_425794 181 213 −R −R NNP 10_1101-2021_01_07_425794 181 214 su su NNP 10_1101-2021_01_07_425794 181 215 br br VBP 10_1101-2021_01_07_425794 181 216 ea ea NN 10_1101-2021_01_07_425794 181 217 d d NN 10_1101-2021_01_07_425794 181 218 E e NN 10_1101-2021_01_07_425794 181 219 ns ns IN 10_1101-2021_01_07_425794 181 220 em -PRON- PRP 10_1101-2021_01_07_425794 181 221 bl bl PRP 10_1101-2021_01_07_425794 181 222 vs vs IN 10_1101-2021_01_07_425794 181 223 R R NNP 10_1101-2021_01_07_425794 181 224 ef ef NNP 10_1101-2021_01_07_425794 181 225 S S NNP 10_1101-2021_01_07_425794 181 226 eq eq NNP 10_1101-2021_01_07_425794 181 227 -N -n NN 10_1101-2021_01_07_425794 181 228 C C NNP 10_1101-2021_01_07_425794 181 229 B B NNP 10_1101-2021_01_07_425794 181 230 I i NN 10_1101-2021_01_07_425794 181 231 C c NN 10_1101-2021_01_07_425794 181 232 E e NN 10_1101-2021_01_07_425794 181 233 D d NN 10_1101-2021_01_07_425794 181 234 iff iff NN 10_1101-2021_01_07_425794 181 235 er er UH 10_1101-2021_01_07_425794 181 236 en en FW 10_1101-2021_01_07_425794 181 237 ce ce NNP 10_1101-2021_01_07_425794 181 238 in in IN 10_1101-2021_01_07_425794 181 239 lo lo NNP 10_1101-2021_01_07_425794 181 240 g2 g2 NNP 10_1101-2021_01_07_425794 181 241 ef ef NNP 10_1101-2021_01_07_425794 181 242 fe fe NNP 10_1101-2021_01_07_425794 181 243 ct ct NNP 10_1101-2021_01_07_425794 181 244 iv iv NNP 10_1101-2021_01_07_425794 181 245 e e NNP 10_1101-2021_01_07_425794 181 246 ge ge NNP 10_1101-2021_01_07_425794 181 247 ne ne NNP 10_1101-2021_01_07_425794 181 248 le le NNP 10_1101-2021_01_07_425794 181 249 ng ng PRP 10_1101-2021_01_07_425794 181 250 th th XX 10_1101-2021_01_07_425794 181 251 Lo Lo NNP 10_1101-2021_01_07_425794 181 252 g2 g2 NNP 10_1101-2021_01_07_425794 181 253 ef ef FW 10_1101-2021_01_07_425794 181 254 fe fe NNP 10_1101-2021_01_07_425794 181 255 ct ct NNP 10_1101-2021_01_07_425794 181 256 iv iv NNP 10_1101-2021_01_07_425794 181 257 e e NNP 10_1101-2021_01_07_425794 181 258 ge ge NNP 10_1101-2021_01_07_425794 181 259 ne ne NNP 10_1101-2021_01_07_425794 181 260 le le NNP 10_1101-2021_01_07_425794 181 261 ng ng NNP 10_1101-2021_01_07_425794 181 262 th th XX 10_1101-2021_01_07_425794 181 263 0 0 CD 10_1101-2021_01_07_425794 181 264 20 20 CD 10_1101-2021_01_07_425794 181 265 40 40 CD 10_1101-2021_01_07_425794 181 266 60 60 CD 10_1101-2021_01_07_425794 181 267 3 3 CD 10_1101-2021_01_07_425794 181 268 6 6 CD 10_1101-2021_01_07_425794 181 269 9 9 CD 10_1101-2021_01_07_425794 181 270 12 12 CD 10_1101-2021_01_07_425794 181 271 15 15 CD 10_1101-2021_01_07_425794 181 272 18 18 CD 10_1101-2021_01_07_425794 181 273 3 3 CD 10_1101-2021_01_07_425794 181 274 6 6 CD 10_1101-2021_01_07_425794 181 275 9 9 CD 10_1101-2021_01_07_425794 181 276 12 12 CD 10_1101-2021_01_07_425794 181 277 15 15 CD 10_1101-2021_01_07_425794 181 278 18 18 CD 10_1101-2021_01_07_425794 181 279 −4 −4 CD 10_1101-2021_01_07_425794 181 280 −2 −2 NNP 10_1101-2021_01_07_425794 181 281 0 0 CD 10_1101-2021_01_07_425794 181 282 2 2 CD 10_1101-2021_01_07_425794 181 283 4 4 CD 10_1101-2021_01_07_425794 181 284 6 6 CD 10_1101-2021_01_07_425794 181 285 −4 −4 CD 10_1101-2021_01_07_425794 181 286 −2 −2 NNP 10_1101-2021_01_07_425794 181 287 0 0 CD 10_1101-2021_01_07_425794 181 288 2 2 CD 10_1101-2021_01_07_425794 181 289 4 4 CD 10_1101-2021_01_07_425794 181 290 6 6 CD 10_1101-2021_01_07_425794 181 291 Figure figure NN 10_1101-2021_01_07_425794 181 292 4 4 CD 10_1101-2021_01_07_425794 181 293 : : : 10_1101-2021_01_07_425794 181 294 Concordance concordance NN 10_1101-2021_01_07_425794 181 295 and and CC 10_1101-2021_01_07_425794 181 296 differences difference NNS 10_1101-2021_01_07_425794 181 297 between between IN 10_1101-2021_01_07_425794 181 298 gene gene NN 10_1101-2021_01_07_425794 181 299 annotations annotation NNS 10_1101-2021_01_07_425794 181 300 after after IN 10_1101-2021_01_07_425794 181 301 filtering filter VBG 10_1101-2021_01_07_425794 181 302 for for IN 10_1101-2021_01_07_425794 181 303 lowly lowly JJ 10_1101-2021_01_07_425794 181 304 expressed express VBN 10_1101-2021_01_07_425794 181 305 genes gene NNS 10_1101-2021_01_07_425794 181 306 . . . 10_1101-2021_01_07_425794 182 1 ( ( -LRB- 10_1101-2021_01_07_425794 182 2 A a NN 10_1101-2021_01_07_425794 182 3 ) ) -RRB- 10_1101-2021_01_07_425794 182 4 Bar bar NN 10_1101-2021_01_07_425794 182 5 plot plot NN 10_1101-2021_01_07_425794 182 6 showing show VBG 10_1101-2021_01_07_425794 182 7 the the DT 10_1101-2021_01_07_425794 182 8 differences difference NNS 10_1101-2021_01_07_425794 182 9 in in IN 10_1101-2021_01_07_425794 182 10 the the DT 10_1101-2021_01_07_425794 182 11 number number NN 10_1101-2021_01_07_425794 182 12 of of IN 10_1101-2021_01_07_425794 182 13 genes gene NNS 10_1101-2021_01_07_425794 182 14 included include VBN 10_1101-2021_01_07_425794 182 15 in in IN 10_1101-2021_01_07_425794 182 16 each each DT 10_1101-2021_01_07_425794 182 17 annotation annotation NN 10_1101-2021_01_07_425794 182 18 before before RB 10_1101-2021_01_07_425794 182 19 and and CC 10_1101-2021_01_07_425794 182 20 after after IN 10_1101-2021_01_07_425794 182 21 filtering filter VBG 10_1101-2021_01_07_425794 182 22 for for IN 10_1101-2021_01_07_425794 182 23 lowly lowly JJ 10_1101-2021_01_07_425794 182 24 expressed express VBN 10_1101-2021_01_07_425794 182 25 genes gene NNS 10_1101-2021_01_07_425794 182 26 . . . 10_1101-2021_01_07_425794 183 1 ( ( -LRB- 10_1101-2021_01_07_425794 183 2 B b NN 10_1101-2021_01_07_425794 183 3 ) ) -RRB- 10_1101-2021_01_07_425794 183 4 Venn Venn NNP 10_1101-2021_01_07_425794 183 5 diagram diagram NN 10_1101-2021_01_07_425794 183 6 comparing comparing NN 10_1101-2021_01_07_425794 183 7 genes gene NNS 10_1101-2021_01_07_425794 183 8 from from IN 10_1101-2021_01_07_425794 183 9 different different JJ 10_1101-2021_01_07_425794 183 10 annotations annotation NNS 10_1101-2021_01_07_425794 183 11 after after IN 10_1101-2021_01_07_425794 183 12 filtering filter VBG 10_1101-2021_01_07_425794 183 13 for for IN 10_1101-2021_01_07_425794 183 14 lowly lowly JJ 10_1101-2021_01_07_425794 183 15 expressed express VBN 10_1101-2021_01_07_425794 183 16 genes gene NNS 10_1101-2021_01_07_425794 183 17 . . . 10_1101-2021_01_07_425794 184 1 Distributions distribution NNS 10_1101-2021_01_07_425794 184 2 of of IN 10_1101-2021_01_07_425794 184 3 effective effective JJ 10_1101-2021_01_07_425794 184 4 gene gene NN 10_1101-2021_01_07_425794 184 5 lengths length NNS 10_1101-2021_01_07_425794 184 6 after after IN 10_1101-2021_01_07_425794 184 7 filtering filtering NN 10_1101-2021_01_07_425794 184 8 are be VBP 10_1101-2021_01_07_425794 184 9 shown show VBN 10_1101-2021_01_07_425794 184 10 for for IN 10_1101-2021_01_07_425794 184 11 all all DT 10_1101-2021_01_07_425794 184 12 genes gene NNS 10_1101-2021_01_07_425794 184 13 in in IN 10_1101-2021_01_07_425794 184 14 each each DT 10_1101-2021_01_07_425794 184 15 annotation annotation NN 10_1101-2021_01_07_425794 184 16 ( ( -LRB- 10_1101-2021_01_07_425794 184 17 C c NN 10_1101-2021_01_07_425794 184 18 ) ) -RRB- 10_1101-2021_01_07_425794 184 19 and and CC 10_1101-2021_01_07_425794 184 20 for for IN 10_1101-2021_01_07_425794 184 21 genes gene NNS 10_1101-2021_01_07_425794 184 22 that that WDT 10_1101-2021_01_07_425794 184 23 are be VBP 10_1101-2021_01_07_425794 184 24 common common JJ 10_1101-2021_01_07_425794 184 25 between between IN 10_1101-2021_01_07_425794 184 26 all all DT 10_1101-2021_01_07_425794 184 27 three three CD 10_1101-2021_01_07_425794 184 28 annotations annotation NNS 10_1101-2021_01_07_425794 184 29 ( ( -LRB- 10_1101-2021_01_07_425794 184 30 D D NNP 10_1101-2021_01_07_425794 184 31 ) ) -RRB- 10_1101-2021_01_07_425794 184 32 . . . 10_1101-2021_01_07_425794 185 1 Distributions distribution NNS 10_1101-2021_01_07_425794 185 2 of of IN 10_1101-2021_01_07_425794 185 3 differences difference NNS 10_1101-2021_01_07_425794 185 4 of of IN 10_1101-2021_01_07_425794 185 5 effective effective JJ 10_1101-2021_01_07_425794 185 6 gene gene NN 10_1101-2021_01_07_425794 185 7 lengths length NNS 10_1101-2021_01_07_425794 185 8 between between IN 10_1101-2021_01_07_425794 185 9 annotations annotation NNS 10_1101-2021_01_07_425794 185 10 after after IN 10_1101-2021_01_07_425794 185 11 filtering filtering NN 10_1101-2021_01_07_425794 185 12 are be VBP 10_1101-2021_01_07_425794 185 13 shown show VBN 10_1101-2021_01_07_425794 185 14 for for IN 10_1101-2021_01_07_425794 185 15 common common JJ 10_1101-2021_01_07_425794 185 16 genes gene NNS 10_1101-2021_01_07_425794 185 17 between between IN 10_1101-2021_01_07_425794 185 18 each each DT 10_1101-2021_01_07_425794 185 19 pair pair NN 10_1101-2021_01_07_425794 185 20 of of IN 10_1101-2021_01_07_425794 185 21 annotations annotation NNS 10_1101-2021_01_07_425794 185 22 ( ( -LRB- 10_1101-2021_01_07_425794 185 23 E E NNP 10_1101-2021_01_07_425794 185 24 ) ) -RRB- 10_1101-2021_01_07_425794 185 25 and and CC 10_1101-2021_01_07_425794 185 26 for for IN 10_1101-2021_01_07_425794 185 27 genes gene NNS 10_1101-2021_01_07_425794 185 28 that that WDT 10_1101-2021_01_07_425794 185 29 are be VBP 10_1101-2021_01_07_425794 185 30 common common JJ 10_1101-2021_01_07_425794 185 31 between between IN 10_1101-2021_01_07_425794 185 32 all all DT 10_1101-2021_01_07_425794 185 33 three three CD 10_1101-2021_01_07_425794 185 34 annotations annotation NNS 10_1101-2021_01_07_425794 185 35 ( ( -LRB- 10_1101-2021_01_07_425794 185 36 F F NNP 10_1101-2021_01_07_425794 185 37 ) ) -RRB- 10_1101-2021_01_07_425794 185 38 . . . 10_1101-2021_01_07_425794 186 1 13 13 CD 10_1101-2021_01_07_425794 186 2 .CC .CC : 10_1101-2021_01_07_425794 186 3 - - HYPH 10_1101-2021_01_07_425794 186 4 BY by IN 10_1101-2021_01_07_425794 186 5 - - HYPH 10_1101-2021_01_07_425794 186 6 NC NC NNP 10_1101-2021_01_07_425794 186 7 - - HYPH 10_1101-2021_01_07_425794 186 8 ND ND NNP 10_1101-2021_01_07_425794 186 9 4.0 4.0 CD 10_1101-2021_01_07_425794 186 10 International international JJ 10_1101-2021_01_07_425794 186 11 licensemade licensemade NN 10_1101-2021_01_07_425794 186 12 available available JJ 10_1101-2021_01_07_425794 186 13 under under IN 10_1101-2021_01_07_425794 186 14 a a DT 10_1101-2021_01_07_425794 186 15 ( ( -LRB- 10_1101-2021_01_07_425794 186 16 which which WDT 10_1101-2021_01_07_425794 186 17 was be VBD 10_1101-2021_01_07_425794 186 18 not not RB 10_1101-2021_01_07_425794 186 19 certified certify VBN 10_1101-2021_01_07_425794 186 20 by by IN 10_1101-2021_01_07_425794 186 21 peer peer NN 10_1101-2021_01_07_425794 186 22 review review NN 10_1101-2021_01_07_425794 186 23 ) ) -RRB- 10_1101-2021_01_07_425794 186 24 is be VBZ 10_1101-2021_01_07_425794 186 25 the the DT 10_1101-2021_01_07_425794 186 26 author author NN 10_1101-2021_01_07_425794 186 27 / / SYM 10_1101-2021_01_07_425794 186 28 funder funder NN 10_1101-2021_01_07_425794 186 29 , , , 10_1101-2021_01_07_425794 186 30 who who WP 10_1101-2021_01_07_425794 186 31 has have VBZ 10_1101-2021_01_07_425794 186 32 granted grant VBN 10_1101-2021_01_07_425794 186 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 186 34 a a DT 10_1101-2021_01_07_425794 186 35 license license NN 10_1101-2021_01_07_425794 186 36 to to TO 10_1101-2021_01_07_425794 186 37 display display VB 10_1101-2021_01_07_425794 186 38 the the DT 10_1101-2021_01_07_425794 186 39 preprint preprint NN 10_1101-2021_01_07_425794 186 40 in in IN 10_1101-2021_01_07_425794 186 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 186 42 . . . 10_1101-2021_01_07_425794 187 1 It -PRON- PRP 10_1101-2021_01_07_425794 187 2 is be VBZ 10_1101-2021_01_07_425794 187 3 The the DT 10_1101-2021_01_07_425794 187 4 copyright copyright NN 10_1101-2021_01_07_425794 187 5 holder holder NN 10_1101-2021_01_07_425794 187 6 for for IN 10_1101-2021_01_07_425794 187 7 this this DT 10_1101-2021_01_07_425794 187 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 187 9 version version NN 10_1101-2021_01_07_425794 187 10 posted post VBD 10_1101-2021_01_07_425794 187 11 January January NNP 10_1101-2021_01_07_425794 187 12 8 8 CD 10_1101-2021_01_07_425794 187 13 , , , 10_1101-2021_01_07_425794 187 14 2021 2021 CD 10_1101-2021_01_07_425794 187 15 . . . 10_1101-2021_01_07_425794 187 16 ; ; : 10_1101-2021_01_07_425794 187 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 187 18 : : : 10_1101-2021_01_07_425794 187 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 187 20 preprint preprint JJ 10_1101-2021_01_07_425794 187 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 187 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 187 23 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 187 24 genes gene NNS 10_1101-2021_01_07_425794 187 25 had have VBD 10_1101-2021_01_07_425794 187 26 the the DT 10_1101-2021_01_07_425794 187 27 shortest short JJS 10_1101-2021_01_07_425794 187 28 gene gene NN 10_1101-2021_01_07_425794 187 29 lengths length NNS 10_1101-2021_01_07_425794 187 30 . . . 10_1101-2021_01_07_425794 188 1 3.5 3.5 CD 10_1101-2021_01_07_425794 188 2 Comparison comparison NN 10_1101-2021_01_07_425794 188 3 of of IN 10_1101-2021_01_07_425794 188 4 titration titration NN 10_1101-2021_01_07_425794 188 5 monotonicity monotonicity NN 10_1101-2021_01_07_425794 188 6 preservation preservation NN 10_1101-2021_01_07_425794 188 7 To to TO 10_1101-2021_01_07_425794 188 8 assess assess VB 10_1101-2021_01_07_425794 188 9 the the DT 10_1101-2021_01_07_425794 188 10 impact impact NN 10_1101-2021_01_07_425794 188 11 of of IN 10_1101-2021_01_07_425794 188 12 gene gene NN 10_1101-2021_01_07_425794 188 13 annotation annotation NN 10_1101-2021_01_07_425794 188 14 choice choice NN 10_1101-2021_01_07_425794 188 15 on on IN 10_1101-2021_01_07_425794 188 16 the the DT 10_1101-2021_01_07_425794 188 17 accuracy accuracy NN 10_1101-2021_01_07_425794 188 18 of of IN 10_1101-2021_01_07_425794 188 19 RNA RNA NNP 10_1101-2021_01_07_425794 188 20 - - HYPH 10_1101-2021_01_07_425794 188 21 seq seq NN 10_1101-2021_01_07_425794 188 22 quantification quantification NN 10_1101-2021_01_07_425794 188 23 result result NN 10_1101-2021_01_07_425794 188 24 , , , 10_1101-2021_01_07_425794 188 25 we -PRON- PRP 10_1101-2021_01_07_425794 188 26 utilized utilize VBD 10_1101-2021_01_07_425794 188 27 as as IN 10_1101-2021_01_07_425794 188 28 ground ground NN 10_1101-2021_01_07_425794 188 29 truth truth NN 10_1101-2021_01_07_425794 188 30 the the DT 10_1101-2021_01_07_425794 188 31 inbuilt inbuilt NN 10_1101-2021_01_07_425794 188 32 titration titration NN 10_1101-2021_01_07_425794 188 33 monotonicity monotonicity NN 10_1101-2021_01_07_425794 188 34 in in IN 10_1101-2021_01_07_425794 188 35 the the DT 10_1101-2021_01_07_425794 188 36 SEQC SEQC NNP 10_1101-2021_01_07_425794 188 37 data datum NNS 10_1101-2021_01_07_425794 188 38 , , , 10_1101-2021_01_07_425794 188 39 the the DT 10_1101-2021_01_07_425794 188 40 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 188 41 RT RT NNP 10_1101-2021_01_07_425794 188 42 - - HYPH 10_1101-2021_01_07_425794 188 43 PCR PCR NNP 10_1101-2021_01_07_425794 188 44 data datum NNS 10_1101-2021_01_07_425794 188 45 and and CC 10_1101-2021_01_07_425794 188 46 the the DT 10_1101-2021_01_07_425794 188 47 microarray microarray JJ 10_1101-2021_01_07_425794 188 48 data datum NNS 10_1101-2021_01_07_425794 188 49 generated generate VBN 10_1101-2021_01_07_425794 188 50 for for IN 10_1101-2021_01_07_425794 188 51 the the DT 10_1101-2021_01_07_425794 188 52 same same JJ 10_1101-2021_01_07_425794 188 53 samples sample NNS 10_1101-2021_01_07_425794 188 54 , , , 10_1101-2021_01_07_425794 188 55 to to TO 10_1101-2021_01_07_425794 188 56 evaluate evaluate VB 10_1101-2021_01_07_425794 188 57 which which WDT 10_1101-2021_01_07_425794 188 58 annotation annotation NN 10_1101-2021_01_07_425794 188 59 gives give VBZ 10_1101-2021_01_07_425794 188 60 rise rise NN 10_1101-2021_01_07_425794 188 61 to to IN 10_1101-2021_01_07_425794 188 62 a a DT 10_1101-2021_01_07_425794 188 63 better well JJR 10_1101-2021_01_07_425794 188 64 expression expression NN 10_1101-2021_01_07_425794 188 65 correlation correlation NN 10_1101-2021_01_07_425794 188 66 of of IN 10_1101-2021_01_07_425794 188 67 the the DT 10_1101-2021_01_07_425794 188 68 RNA RNA NNP 10_1101-2021_01_07_425794 188 69 - - HYPH 10_1101-2021_01_07_425794 188 70 seq seq NN 10_1101-2021_01_07_425794 188 71 quantification quantification NN 10_1101-2021_01_07_425794 188 72 data datum NNS 10_1101-2021_01_07_425794 188 73 with with IN 10_1101-2021_01_07_425794 188 74 the the DT 10_1101-2021_01_07_425794 188 75 truth truth NN 10_1101-2021_01_07_425794 188 76 . . . 10_1101-2021_01_07_425794 189 1 In in IN 10_1101-2021_01_07_425794 189 2 this this DT 10_1101-2021_01_07_425794 189 3 section section NN 10_1101-2021_01_07_425794 189 4 , , , 10_1101-2021_01_07_425794 189 5 we -PRON- PRP 10_1101-2021_01_07_425794 189 6 compared compare VBD 10_1101-2021_01_07_425794 189 7 the the DT 10_1101-2021_01_07_425794 189 8 ability ability NN 10_1101-2021_01_07_425794 189 9 of of IN 10_1101-2021_01_07_425794 189 10 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 189 11 and and CC 10_1101-2021_01_07_425794 189 12 the the DT 10_1101-2021_01_07_425794 189 13 two two CD 10_1101-2021_01_07_425794 189 14 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 189 15 annotations annotation NNS 10_1101-2021_01_07_425794 189 16 in in IN 10_1101-2021_01_07_425794 189 17 retaining retain VBG 10_1101-2021_01_07_425794 189 18 the the DT 10_1101-2021_01_07_425794 189 19 inbuilt inbuilt NN 10_1101-2021_01_07_425794 189 20 titration titration NN 10_1101-2021_01_07_425794 189 21 monotonicity monotonicity NN 10_1101-2021_01_07_425794 189 22 in in IN 10_1101-2021_01_07_425794 189 23 the the DT 10_1101-2021_01_07_425794 189 24 RNA RNA NNP 10_1101-2021_01_07_425794 189 25 - - HYPH 10_1101-2021_01_07_425794 189 26 seq seq NN 10_1101-2021_01_07_425794 189 27 dataset dataset NN 10_1101-2021_01_07_425794 189 28 . . . 10_1101-2021_01_07_425794 190 1 In in IN 10_1101-2021_01_07_425794 190 2 Figure Figure NNP 10_1101-2021_01_07_425794 190 3 5 5 CD 10_1101-2021_01_07_425794 190 4 , , , 10_1101-2021_01_07_425794 190 5 the the DT 10_1101-2021_01_07_425794 190 6 reference reference NN 10_1101-2021_01_07_425794 190 7 titration titration NN 10_1101-2021_01_07_425794 190 8 curve curve NN 10_1101-2021_01_07_425794 190 9 depicts depict VBZ 10_1101-2021_01_07_425794 190 10 the the DT 10_1101-2021_01_07_425794 190 11 expected expect VBN 10_1101-2021_01_07_425794 190 12 fold fold JJ 10_1101-2021_01_07_425794 190 13 change change NN 10_1101-2021_01_07_425794 190 14 that that IN 10_1101-2021_01_07_425794 190 15 genes gene NNS 10_1101-2021_01_07_425794 190 16 are be VBP 10_1101-2021_01_07_425794 190 17 expected expect VBN 10_1101-2021_01_07_425794 190 18 to to TO 10_1101-2021_01_07_425794 190 19 follow follow VB 10_1101-2021_01_07_425794 190 20 in in IN 10_1101-2021_01_07_425794 190 21 sample sample NN 10_1101-2021_01_07_425794 190 22 C C NNP 10_1101-2021_01_07_425794 190 23 vs vs IN 10_1101-2021_01_07_425794 190 24 sample sample NN 10_1101-2021_01_07_425794 190 25 D d NN 10_1101-2021_01_07_425794 190 26 based base VBN 10_1101-2021_01_07_425794 190 27 on on IN 10_1101-2021_01_07_425794 190 28 the the DT 10_1101-2021_01_07_425794 190 29 fold fold JJ 10_1101-2021_01_07_425794 190 30 change change NN 10_1101-2021_01_07_425794 190 31 in in IN 10_1101-2021_01_07_425794 190 32 sample sample NN 10_1101-2021_01_07_425794 190 33 A A NNP 10_1101-2021_01_07_425794 190 34 vs vs FW 10_1101-2021_01_07_425794 190 35 sample sample NN 10_1101-2021_01_07_425794 190 36 B. b. NN 10_1101-2021_01_07_425794 191 1 This this DT 10_1101-2021_01_07_425794 191 2 is be VBZ 10_1101-2021_01_07_425794 191 3 computed compute VBN 10_1101-2021_01_07_425794 191 4 using use VBG 10_1101-2021_01_07_425794 191 5 the the DT 10_1101-2021_01_07_425794 191 6 Equation Equation NNP 10_1101-2021_01_07_425794 191 7 ( ( -LRB- 10_1101-2021_01_07_425794 191 8 1 1 CD 10_1101-2021_01_07_425794 191 9 ) ) -RRB- 10_1101-2021_01_07_425794 191 10 ( ( -LRB- 10_1101-2021_01_07_425794 191 11 see see VB 10_1101-2021_01_07_425794 191 12 MATERIALS material NNS 10_1101-2021_01_07_425794 191 13 AND and CC 10_1101-2021_01_07_425794 191 14 METHODS method NNS 10_1101-2021_01_07_425794 191 15 ) ) -RRB- 10_1101-2021_01_07_425794 191 16 . . . 10_1101-2021_01_07_425794 192 1 We -PRON- PRP 10_1101-2021_01_07_425794 192 2 then then RB 10_1101-2021_01_07_425794 192 3 calculated calculate VBD 10_1101-2021_01_07_425794 192 4 the the DT 10_1101-2021_01_07_425794 192 5 Mean Mean NNP 10_1101-2021_01_07_425794 192 6 Squared Squared NNP 10_1101-2021_01_07_425794 192 7 Error Error NNP 10_1101-2021_01_07_425794 192 8 ( ( -LRB- 10_1101-2021_01_07_425794 192 9 MSE MSE NNP 10_1101-2021_01_07_425794 192 10 ) ) -RRB- 10_1101-2021_01_07_425794 192 11 between between IN 10_1101-2021_01_07_425794 192 12 the the DT 10_1101-2021_01_07_425794 192 13 reference reference NN 10_1101-2021_01_07_425794 192 14 titration titration NN 10_1101-2021_01_07_425794 192 15 monotonicity monotonicity NN 10_1101-2021_01_07_425794 192 16 and and CC 10_1101-2021_01_07_425794 192 17 the the DT 10_1101-2021_01_07_425794 192 18 titration titration NN 10_1101-2021_01_07_425794 192 19 monotonicity monotonicity NN 10_1101-2021_01_07_425794 192 20 obtained obtain VBN 10_1101-2021_01_07_425794 192 21 from from IN 10_1101-2021_01_07_425794 192 22 each each DT 10_1101-2021_01_07_425794 192 23 annotation annotation NN 10_1101-2021_01_07_425794 192 24 . . . 10_1101-2021_01_07_425794 193 1 A a DT 10_1101-2021_01_07_425794 193 2 smaller small JJR 10_1101-2021_01_07_425794 193 3 MSE mse NN 10_1101-2021_01_07_425794 193 4 value value NN 10_1101-2021_01_07_425794 193 5 means mean VBZ 10_1101-2021_01_07_425794 193 6 that that IN 10_1101-2021_01_07_425794 193 7 the the DT 10_1101-2021_01_07_425794 193 8 generated generate VBN 10_1101-2021_01_07_425794 193 9 quantification quantification NN 10_1101-2021_01_07_425794 193 10 data datum NNS 10_1101-2021_01_07_425794 193 11 is be VBZ 10_1101-2021_01_07_425794 193 12 closer close JJR 10_1101-2021_01_07_425794 193 13 to to IN 10_1101-2021_01_07_425794 193 14 the the DT 10_1101-2021_01_07_425794 193 15 truth truth NN 10_1101-2021_01_07_425794 193 16 . . . 10_1101-2021_01_07_425794 194 1 Figure figure NN 10_1101-2021_01_07_425794 194 2 5 5 CD 10_1101-2021_01_07_425794 194 3 shows show VBZ 10_1101-2021_01_07_425794 194 4 that that IN 10_1101-2021_01_07_425794 194 5 the the DT 10_1101-2021_01_07_425794 194 6 MSE MSE NNP 10_1101-2021_01_07_425794 194 7 computed compute VBD 10_1101-2021_01_07_425794 194 8 for for IN 10_1101-2021_01_07_425794 194 9 the the DT 10_1101-2021_01_07_425794 194 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 194 11 - - HYPH 10_1101-2021_01_07_425794 194 12 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 194 13 annotation annotation NN 10_1101-2021_01_07_425794 194 14 is be VBZ 10_1101-2021_01_07_425794 194 15 constantly constantly RB 10_1101-2021_01_07_425794 194 16 lower low JJR 10_1101-2021_01_07_425794 194 17 than than IN 10_1101-2021_01_07_425794 194 18 those those DT 10_1101-2021_01_07_425794 194 19 computed compute VBN 10_1101-2021_01_07_425794 194 20 for for IN 10_1101-2021_01_07_425794 194 21 the the DT 10_1101-2021_01_07_425794 194 22 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 194 23 and and CC 10_1101-2021_01_07_425794 194 24 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 194 25 - - HYPH 10_1101-2021_01_07_425794 194 26 NCBI NCBI NNP 10_1101-2021_01_07_425794 194 27 annotations annotation NNS 10_1101-2021_01_07_425794 194 28 , , , 10_1101-2021_01_07_425794 194 29 regardless regardless RB 10_1101-2021_01_07_425794 194 30 if if IN 10_1101-2021_01_07_425794 194 31 filtering filtering NN 10_1101-2021_01_07_425794 194 32 was be VBD 10_1101-2021_01_07_425794 194 33 applied apply VBN 10_1101-2021_01_07_425794 194 34 or or CC 10_1101-2021_01_07_425794 194 35 if if IN 10_1101-2021_01_07_425794 194 36 only only RB 10_1101-2021_01_07_425794 194 37 common common JJ 10_1101-2021_01_07_425794 194 38 genes gene NNS 10_1101-2021_01_07_425794 194 39 were be VBD 10_1101-2021_01_07_425794 194 40 included include VBN 10_1101-2021_01_07_425794 194 41 for for IN 10_1101-2021_01_07_425794 194 42 comparison comparison NN 10_1101-2021_01_07_425794 194 43 . . . 10_1101-2021_01_07_425794 195 1 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 195 2 - - HYPH 10_1101-2021_01_07_425794 195 3 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 195 4 was be VBD 10_1101-2021_01_07_425794 195 5 also also RB 10_1101-2021_01_07_425794 195 6 found find VBN 10_1101-2021_01_07_425794 195 7 to to TO 10_1101-2021_01_07_425794 195 8 yield yield VB 10_1101-2021_01_07_425794 195 9 comparable comparable JJ 10_1101-2021_01_07_425794 195 10 or or CC 10_1101-2021_01_07_425794 195 11 lower low JJR 10_1101-2021_01_07_425794 195 12 MSE MSE NNP 10_1101-2021_01_07_425794 195 13 compared compare VBN 10_1101-2021_01_07_425794 195 14 to to IN 10_1101-2021_01_07_425794 195 15 the the DT 10_1101-2021_01_07_425794 195 16 other other JJ 10_1101-2021_01_07_425794 195 17 two two CD 10_1101-2021_01_07_425794 195 18 annotations annotation NNS 10_1101-2021_01_07_425794 195 19 when when WRB 10_1101-2021_01_07_425794 195 20 the the DT 10_1101-2021_01_07_425794 195 21 data datum NNS 10_1101-2021_01_07_425794 195 22 were be VBD 10_1101-2021_01_07_425794 195 23 TMM TMM NNP 10_1101-2021_01_07_425794 195 24 or or CC 10_1101-2021_01_07_425794 195 25 quantile quantile NN 10_1101-2021_01_07_425794 195 26 normalized normalized JJ 10_1101-2021_01_07_425794 195 27 ( ( -LRB- 10_1101-2021_01_07_425794 195 28 Supplementary Supplementary NNP 10_1101-2021_01_07_425794 195 29 Figures Figures NNPS 10_1101-2021_01_07_425794 195 30 S4 S4 NNP 10_1101-2021_01_07_425794 195 31 and and CC 10_1101-2021_01_07_425794 195 32 S5 s5 NN 10_1101-2021_01_07_425794 195 33 ) ) -RRB- 10_1101-2021_01_07_425794 195 34 , , , 10_1101-2021_01_07_425794 195 35 in in IN 10_1101-2021_01_07_425794 195 36 addition addition NN 10_1101-2021_01_07_425794 195 37 to to IN 10_1101-2021_01_07_425794 195 38 the the DT 10_1101-2021_01_07_425794 195 39 library library NN 10_1101-2021_01_07_425794 195 40 - - HYPH 10_1101-2021_01_07_425794 195 41 size size NN 10_1101-2021_01_07_425794 195 42 normalized normalize VBN 10_1101-2021_01_07_425794 195 43 data datum NNS 10_1101-2021_01_07_425794 195 44 shown show VBN 10_1101-2021_01_07_425794 195 45 in in IN 10_1101-2021_01_07_425794 195 46 Figure Figure NNP 10_1101-2021_01_07_425794 195 47 5 5 CD 10_1101-2021_01_07_425794 195 48 . . . 10_1101-2021_01_07_425794 196 1 These these DT 10_1101-2021_01_07_425794 196 2 results result NNS 10_1101-2021_01_07_425794 196 3 demonstrated demonstrate VBD 10_1101-2021_01_07_425794 196 4 that that IN 10_1101-2021_01_07_425794 196 5 the the DT 10_1101-2021_01_07_425794 196 6 use use NN 10_1101-2021_01_07_425794 196 7 of of IN 10_1101-2021_01_07_425794 196 8 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 196 9 - - HYPH 10_1101-2021_01_07_425794 196 10 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 196 11 annotation annotation NN 10_1101-2021_01_07_425794 196 12 led lead VBD 10_1101-2021_01_07_425794 196 13 to to IN 10_1101-2021_01_07_425794 196 14 better well JJR 10_1101-2021_01_07_425794 196 15 quantification quantification NN 10_1101-2021_01_07_425794 196 16 accuracy accuracy NN 10_1101-2021_01_07_425794 196 17 for for IN 10_1101-2021_01_07_425794 196 18 the the DT 10_1101-2021_01_07_425794 196 19 RNA RNA NNP 10_1101-2021_01_07_425794 196 20 - - HYPH 10_1101-2021_01_07_425794 196 21 seq seq NN 10_1101-2021_01_07_425794 196 22 data datum NNS 10_1101-2021_01_07_425794 196 23 . . . 10_1101-2021_01_07_425794 197 1 14 14 CD 10_1101-2021_01_07_425794 197 2 .CC .CC : 10_1101-2021_01_07_425794 197 3 - - HYPH 10_1101-2021_01_07_425794 197 4 BY by IN 10_1101-2021_01_07_425794 197 5 - - HYPH 10_1101-2021_01_07_425794 197 6 NC NC NNP 10_1101-2021_01_07_425794 197 7 - - HYPH 10_1101-2021_01_07_425794 197 8 ND ND NNP 10_1101-2021_01_07_425794 197 9 4.0 4.0 CD 10_1101-2021_01_07_425794 197 10 International international JJ 10_1101-2021_01_07_425794 197 11 licensemade licensemade NN 10_1101-2021_01_07_425794 197 12 available available JJ 10_1101-2021_01_07_425794 197 13 under under IN 10_1101-2021_01_07_425794 197 14 a a DT 10_1101-2021_01_07_425794 197 15 ( ( -LRB- 10_1101-2021_01_07_425794 197 16 which which WDT 10_1101-2021_01_07_425794 197 17 was be VBD 10_1101-2021_01_07_425794 197 18 not not RB 10_1101-2021_01_07_425794 197 19 certified certify VBN 10_1101-2021_01_07_425794 197 20 by by IN 10_1101-2021_01_07_425794 197 21 peer peer NN 10_1101-2021_01_07_425794 197 22 review review NN 10_1101-2021_01_07_425794 197 23 ) ) -RRB- 10_1101-2021_01_07_425794 197 24 is be VBZ 10_1101-2021_01_07_425794 197 25 the the DT 10_1101-2021_01_07_425794 197 26 author author NN 10_1101-2021_01_07_425794 197 27 / / SYM 10_1101-2021_01_07_425794 197 28 funder funder NN 10_1101-2021_01_07_425794 197 29 , , , 10_1101-2021_01_07_425794 197 30 who who WP 10_1101-2021_01_07_425794 197 31 has have VBZ 10_1101-2021_01_07_425794 197 32 granted grant VBN 10_1101-2021_01_07_425794 197 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 197 34 a a DT 10_1101-2021_01_07_425794 197 35 license license NN 10_1101-2021_01_07_425794 197 36 to to TO 10_1101-2021_01_07_425794 197 37 display display VB 10_1101-2021_01_07_425794 197 38 the the DT 10_1101-2021_01_07_425794 197 39 preprint preprint NN 10_1101-2021_01_07_425794 197 40 in in IN 10_1101-2021_01_07_425794 197 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 197 42 . . . 10_1101-2021_01_07_425794 198 1 It -PRON- PRP 10_1101-2021_01_07_425794 198 2 is be VBZ 10_1101-2021_01_07_425794 198 3 The the DT 10_1101-2021_01_07_425794 198 4 copyright copyright NN 10_1101-2021_01_07_425794 198 5 holder holder NN 10_1101-2021_01_07_425794 198 6 for for IN 10_1101-2021_01_07_425794 198 7 this this DT 10_1101-2021_01_07_425794 198 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 198 9 version version NN 10_1101-2021_01_07_425794 198 10 posted post VBD 10_1101-2021_01_07_425794 198 11 January January NNP 10_1101-2021_01_07_425794 198 12 8 8 CD 10_1101-2021_01_07_425794 198 13 , , , 10_1101-2021_01_07_425794 198 14 2021 2021 CD 10_1101-2021_01_07_425794 198 15 . . . 10_1101-2021_01_07_425794 198 16 ; ; : 10_1101-2021_01_07_425794 198 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 198 18 : : : 10_1101-2021_01_07_425794 198 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 198 20 preprint preprint JJ 10_1101-2021_01_07_425794 198 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 198 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 198 23 Figure figure NN 10_1101-2021_01_07_425794 198 24 5 5 CD 10_1101-2021_01_07_425794 198 25 : : : 10_1101-2021_01_07_425794 198 26 Titration titration NN 10_1101-2021_01_07_425794 198 27 monotonicity monotonicity NN 10_1101-2021_01_07_425794 198 28 plots plot NNS 10_1101-2021_01_07_425794 198 29 . . . 10_1101-2021_01_07_425794 199 1 The the DT 10_1101-2021_01_07_425794 199 2 ability ability NN 10_1101-2021_01_07_425794 199 3 of of IN 10_1101-2021_01_07_425794 199 4 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 199 5 , , , 10_1101-2021_01_07_425794 199 6 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 199 7 - - HYPH 10_1101-2021_01_07_425794 199 8 NCBI NCBI NNP 10_1101-2021_01_07_425794 199 9 and and CC 10_1101-2021_01_07_425794 199 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 199 11 - - HYPH 10_1101-2021_01_07_425794 199 12 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 199 13 to to TO 10_1101-2021_01_07_425794 199 14 retain retain VB 10_1101-2021_01_07_425794 199 15 the the DT 10_1101-2021_01_07_425794 199 16 titration titration NN 10_1101-2021_01_07_425794 199 17 monotonicity monotonicity NN 10_1101-2021_01_07_425794 199 18 built build VBN 10_1101-2021_01_07_425794 199 19 into into IN 10_1101-2021_01_07_425794 199 20 the the DT 10_1101-2021_01_07_425794 199 21 SEQC SEQC NNP 10_1101-2021_01_07_425794 199 22 RNA RNA NNP 10_1101-2021_01_07_425794 199 23 - - HYPH 10_1101-2021_01_07_425794 199 24 seq seq NN 10_1101-2021_01_07_425794 199 25 data datum NNS 10_1101-2021_01_07_425794 199 26 was be VBD 10_1101-2021_01_07_425794 199 27 measured measure VBN 10_1101-2021_01_07_425794 199 28 using use VBG 10_1101-2021_01_07_425794 199 29 the the DT 10_1101-2021_01_07_425794 199 30 Mean Mean NNP 10_1101-2021_01_07_425794 199 31 Squared Squared NNP 10_1101-2021_01_07_425794 199 32 Error Error NNP 10_1101-2021_01_07_425794 199 33 ( ( -LRB- 10_1101-2021_01_07_425794 199 34 MSE MSE NNP 10_1101-2021_01_07_425794 199 35 ) ) -RRB- 10_1101-2021_01_07_425794 199 36 between between IN 10_1101-2021_01_07_425794 199 37 the the DT 10_1101-2021_01_07_425794 199 38 reference reference NN 10_1101-2021_01_07_425794 199 39 titration titration NN 10_1101-2021_01_07_425794 199 40 and and CC 10_1101-2021_01_07_425794 199 41 the the DT 10_1101-2021_01_07_425794 199 42 actual actual JJ 10_1101-2021_01_07_425794 199 43 titration titration NN 10_1101-2021_01_07_425794 199 44 obtained obtain VBN 10_1101-2021_01_07_425794 199 45 from from IN 10_1101-2021_01_07_425794 199 46 each each DT 10_1101-2021_01_07_425794 199 47 an- an- RB 10_1101-2021_01_07_425794 199 48 notation notation NN 10_1101-2021_01_07_425794 199 49 . . . 10_1101-2021_01_07_425794 200 1 The the DT 10_1101-2021_01_07_425794 200 2 red red JJ 10_1101-2021_01_07_425794 200 3 curve curve NN 10_1101-2021_01_07_425794 200 4 in in IN 10_1101-2021_01_07_425794 200 5 each each DT 10_1101-2021_01_07_425794 200 6 plot plot NN 10_1101-2021_01_07_425794 200 7 represents represent VBZ 10_1101-2021_01_07_425794 200 8 the the DT 10_1101-2021_01_07_425794 200 9 reference reference NN 10_1101-2021_01_07_425794 200 10 titration titration NN 10_1101-2021_01_07_425794 200 11 calculated calculate VBN 10_1101-2021_01_07_425794 200 12 from from IN 10_1101-2021_01_07_425794 200 13 using use VBG 10_1101-2021_01_07_425794 200 14 Equation Equation NNP 10_1101-2021_01_07_425794 200 15 ( ( -LRB- 10_1101-2021_01_07_425794 200 16 1 1 CD 10_1101-2021_01_07_425794 200 17 ) ) -RRB- 10_1101-2021_01_07_425794 200 18 . . . 10_1101-2021_01_07_425794 201 1 Plots plot NNS 10_1101-2021_01_07_425794 201 2 in in IN 10_1101-2021_01_07_425794 201 3 the the DT 10_1101-2021_01_07_425794 201 4 top top NN 10_1101-2021_01_07_425794 201 5 row row NN 10_1101-2021_01_07_425794 201 6 include include VBP 10_1101-2021_01_07_425794 201 7 all all PDT 10_1101-2021_01_07_425794 201 8 the the DT 10_1101-2021_01_07_425794 201 9 genes gene NNS 10_1101-2021_01_07_425794 201 10 available available JJ 10_1101-2021_01_07_425794 201 11 in in IN 10_1101-2021_01_07_425794 201 12 each each DT 10_1101-2021_01_07_425794 201 13 annotation annotation NN 10_1101-2021_01_07_425794 201 14 . . . 10_1101-2021_01_07_425794 202 1 Plots plot NNS 10_1101-2021_01_07_425794 202 2 in in IN 10_1101-2021_01_07_425794 202 3 the the DT 10_1101-2021_01_07_425794 202 4 middle middle JJ 10_1101-2021_01_07_425794 202 5 row row NN 10_1101-2021_01_07_425794 202 6 includes include VBZ 10_1101-2021_01_07_425794 202 7 those those DT 10_1101-2021_01_07_425794 202 8 genes gene NNS 10_1101-2021_01_07_425794 202 9 that that WDT 10_1101-2021_01_07_425794 202 10 remained remain VBD 10_1101-2021_01_07_425794 202 11 after after IN 10_1101-2021_01_07_425794 202 12 filtering filter VBG 10_1101-2021_01_07_425794 202 13 for for IN 10_1101-2021_01_07_425794 202 14 lowly lowly JJ 10_1101-2021_01_07_425794 202 15 expressed express VBN 10_1101-2021_01_07_425794 202 16 genes gene NNS 10_1101-2021_01_07_425794 202 17 , , , 10_1101-2021_01_07_425794 202 18 in in IN 10_1101-2021_01_07_425794 202 19 each each DT 10_1101-2021_01_07_425794 202 20 annotation annotation NN 10_1101-2021_01_07_425794 202 21 . . . 10_1101-2021_01_07_425794 203 1 Plots plot NNS 10_1101-2021_01_07_425794 203 2 in in IN 10_1101-2021_01_07_425794 203 3 the the DT 10_1101-2021_01_07_425794 203 4 bottom bottom NN 10_1101-2021_01_07_425794 203 5 row row NN 10_1101-2021_01_07_425794 203 6 includes include VBZ 10_1101-2021_01_07_425794 203 7 genes gene NNS 10_1101-2021_01_07_425794 203 8 that that WDT 10_1101-2021_01_07_425794 203 9 are be VBP 10_1101-2021_01_07_425794 203 10 common common JJ 10_1101-2021_01_07_425794 203 11 between between IN 10_1101-2021_01_07_425794 203 12 the the DT 10_1101-2021_01_07_425794 203 13 three three CD 10_1101-2021_01_07_425794 203 14 annotations annotation NNS 10_1101-2021_01_07_425794 203 15 after after IN 10_1101-2021_01_07_425794 203 16 the the DT 10_1101-2021_01_07_425794 203 17 expression expression NN 10_1101-2021_01_07_425794 203 18 filtering filtering NN 10_1101-2021_01_07_425794 203 19 was be VBD 10_1101-2021_01_07_425794 203 20 performed perform VBN 10_1101-2021_01_07_425794 203 21 . . . 10_1101-2021_01_07_425794 204 1 In in IN 10_1101-2021_01_07_425794 204 2 each each DT 10_1101-2021_01_07_425794 204 3 plot plot NN 10_1101-2021_01_07_425794 204 4 , , , 10_1101-2021_01_07_425794 204 5 the the DT 10_1101-2021_01_07_425794 204 6 horizontal horizontal JJ 10_1101-2021_01_07_425794 204 7 axis axis NNP 10_1101-2021_01_07_425794 204 8 represents represent VBZ 10_1101-2021_01_07_425794 204 9 the the DT 10_1101-2021_01_07_425794 204 10 log2 log2 NN 10_1101-2021_01_07_425794 204 11 fold fold VB 10_1101-2021_01_07_425794 204 12 changes change NNS 10_1101-2021_01_07_425794 204 13 of of IN 10_1101-2021_01_07_425794 204 14 gene gene NN 10_1101-2021_01_07_425794 204 15 expres- expres- XX 10_1101-2021_01_07_425794 204 16 sion sion NN 10_1101-2021_01_07_425794 204 17 between between IN 10_1101-2021_01_07_425794 204 18 samples sample NNS 10_1101-2021_01_07_425794 204 19 A a NN 10_1101-2021_01_07_425794 204 20 and and CC 10_1101-2021_01_07_425794 204 21 B b NN 10_1101-2021_01_07_425794 204 22 and and CC 10_1101-2021_01_07_425794 204 23 the the DT 10_1101-2021_01_07_425794 204 24 vertical vertical JJ 10_1101-2021_01_07_425794 204 25 axis axi NNS 10_1101-2021_01_07_425794 204 26 represents represent VBZ 10_1101-2021_01_07_425794 204 27 the the DT 10_1101-2021_01_07_425794 204 28 log2 log2 NN 10_1101-2021_01_07_425794 204 29 fold fold VB 10_1101-2021_01_07_425794 204 30 changes change NNS 10_1101-2021_01_07_425794 204 31 of of IN 10_1101-2021_01_07_425794 204 32 gene gene NN 10_1101-2021_01_07_425794 204 33 expression expression NN 10_1101-2021_01_07_425794 204 34 between between IN 10_1101-2021_01_07_425794 204 35 samples sample NNS 10_1101-2021_01_07_425794 204 36 C C NNP 10_1101-2021_01_07_425794 204 37 and and CC 10_1101-2021_01_07_425794 204 38 D. D. NNP 10_1101-2021_01_07_425794 204 39 3.6 3.6 CD 10_1101-2021_01_07_425794 204 40 Validation validation NN 10_1101-2021_01_07_425794 204 41 against against IN 10_1101-2021_01_07_425794 204 42 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 204 43 RT RT NNP 10_1101-2021_01_07_425794 204 44 - - HYPH 10_1101-2021_01_07_425794 204 45 PCR PCR NNP 10_1101-2021_01_07_425794 204 46 data datum NNS 10_1101-2021_01_07_425794 204 47 The the DT 10_1101-2021_01_07_425794 204 48 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 204 49 RT RT NNP 10_1101-2021_01_07_425794 204 50 - - HYPH 10_1101-2021_01_07_425794 204 51 PCR PCR NNP 10_1101-2021_01_07_425794 204 52 dataset dataset NN 10_1101-2021_01_07_425794 204 53 generated generate VBN 10_1101-2021_01_07_425794 204 54 in in IN 10_1101-2021_01_07_425794 204 55 the the DT 10_1101-2021_01_07_425794 204 56 MAQC MAQC NNP 10_1101-2021_01_07_425794 204 57 study study NN 10_1101-2021_01_07_425794 204 58 [ [ -LRB- 10_1101-2021_01_07_425794 204 59 14 14 CD 10_1101-2021_01_07_425794 204 60 , , , 10_1101-2021_01_07_425794 204 61 15 15 CD 10_1101-2021_01_07_425794 204 62 ] ] -RRB- 10_1101-2021_01_07_425794 204 63 was be VBD 10_1101-2021_01_07_425794 204 64 used use VBN 10_1101-2021_01_07_425794 204 65 to to TO 10_1101-2021_01_07_425794 204 66 validate validate VB 10_1101-2021_01_07_425794 204 67 the the DT 10_1101-2021_01_07_425794 204 68 gene gene NN 10_1101-2021_01_07_425794 204 69 - - HYPH 10_1101-2021_01_07_425794 204 70 level level NN 10_1101-2021_01_07_425794 204 71 quantification quantification NN 10_1101-2021_01_07_425794 204 72 results result NNS 10_1101-2021_01_07_425794 204 73 from from IN 10_1101-2021_01_07_425794 204 74 the the DT 10_1101-2021_01_07_425794 204 75 RNA RNA NNP 10_1101-2021_01_07_425794 204 76 - - HYPH 10_1101-2021_01_07_425794 204 77 seq seq NN 10_1101-2021_01_07_425794 204 78 dataset dataset NN 10_1101-2021_01_07_425794 204 79 . . . 10_1101-2021_01_07_425794 205 1 This this DT 10_1101-2021_01_07_425794 205 2 dataset dataset NN 10_1101-2021_01_07_425794 205 3 contains contain VBZ 10_1101-2021_01_07_425794 205 4 measured measured JJ 10_1101-2021_01_07_425794 205 5 expression expression NN 10_1101-2021_01_07_425794 205 6 levels level NNS 10_1101-2021_01_07_425794 205 7 for for IN 10_1101-2021_01_07_425794 205 8 > > XX 10_1101-2021_01_07_425794 205 9 1,000 1,000 CD 10_1101-2021_01_07_425794 205 10 genes gene NNS 10_1101-2021_01_07_425794 205 11 in in IN 10_1101-2021_01_07_425794 205 12 the the DT 10_1101-2021_01_07_425794 205 13 four four CD 10_1101-2021_01_07_425794 205 14 SEQC SEQC NNP 10_1101-2021_01_07_425794 205 15 samples sample NNS 10_1101-2021_01_07_425794 205 16 . . . 10_1101-2021_01_07_425794 206 1 The the DT 10_1101-2021_01_07_425794 206 2 aim aim NN 10_1101-2021_01_07_425794 206 3 was be VBD 10_1101-2021_01_07_425794 206 4 to to TO 10_1101-2021_01_07_425794 206 5 understand understand VB 10_1101-2021_01_07_425794 206 6 how how WRB 10_1101-2021_01_07_425794 206 7 well well RB 10_1101-2021_01_07_425794 206 8 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 206 9 and and CC 10_1101-2021_01_07_425794 206 10 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 206 11 annotated annotate VBD 10_1101-2021_01_07_425794 206 12 gene gene NN 10_1101-2021_01_07_425794 206 13 expression expression NN 10_1101-2021_01_07_425794 206 14 correlated correlate VBD 10_1101-2021_01_07_425794 206 15 with with IN 10_1101-2021_01_07_425794 206 16 the the DT 10_1101-2021_01_07_425794 206 17 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 206 18 RT RT NNP 10_1101-2021_01_07_425794 206 19 - - HYPH 10_1101-2021_01_07_425794 206 20 PCR PCR NNP 10_1101-2021_01_07_425794 206 21 data datum NNS 10_1101-2021_01_07_425794 206 22 . . . 10_1101-2021_01_07_425794 207 1 15 15 CD 10_1101-2021_01_07_425794 207 2 .CC .CC : 10_1101-2021_01_07_425794 207 3 - - HYPH 10_1101-2021_01_07_425794 207 4 BY by IN 10_1101-2021_01_07_425794 207 5 - - HYPH 10_1101-2021_01_07_425794 207 6 NC NC NNP 10_1101-2021_01_07_425794 207 7 - - HYPH 10_1101-2021_01_07_425794 207 8 ND ND NNP 10_1101-2021_01_07_425794 207 9 4.0 4.0 CD 10_1101-2021_01_07_425794 207 10 International international JJ 10_1101-2021_01_07_425794 207 11 licensemade licensemade NN 10_1101-2021_01_07_425794 207 12 available available JJ 10_1101-2021_01_07_425794 207 13 under under IN 10_1101-2021_01_07_425794 207 14 a a DT 10_1101-2021_01_07_425794 207 15 ( ( -LRB- 10_1101-2021_01_07_425794 207 16 which which WDT 10_1101-2021_01_07_425794 207 17 was be VBD 10_1101-2021_01_07_425794 207 18 not not RB 10_1101-2021_01_07_425794 207 19 certified certify VBN 10_1101-2021_01_07_425794 207 20 by by IN 10_1101-2021_01_07_425794 207 21 peer peer NN 10_1101-2021_01_07_425794 207 22 review review NN 10_1101-2021_01_07_425794 207 23 ) ) -RRB- 10_1101-2021_01_07_425794 207 24 is be VBZ 10_1101-2021_01_07_425794 207 25 the the DT 10_1101-2021_01_07_425794 207 26 author author NN 10_1101-2021_01_07_425794 207 27 / / SYM 10_1101-2021_01_07_425794 207 28 funder funder NN 10_1101-2021_01_07_425794 207 29 , , , 10_1101-2021_01_07_425794 207 30 who who WP 10_1101-2021_01_07_425794 207 31 has have VBZ 10_1101-2021_01_07_425794 207 32 granted grant VBN 10_1101-2021_01_07_425794 207 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 207 34 a a DT 10_1101-2021_01_07_425794 207 35 license license NN 10_1101-2021_01_07_425794 207 36 to to TO 10_1101-2021_01_07_425794 207 37 display display VB 10_1101-2021_01_07_425794 207 38 the the DT 10_1101-2021_01_07_425794 207 39 preprint preprint NN 10_1101-2021_01_07_425794 207 40 in in IN 10_1101-2021_01_07_425794 207 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 207 42 . . . 10_1101-2021_01_07_425794 208 1 It -PRON- PRP 10_1101-2021_01_07_425794 208 2 is be VBZ 10_1101-2021_01_07_425794 208 3 The the DT 10_1101-2021_01_07_425794 208 4 copyright copyright NN 10_1101-2021_01_07_425794 208 5 holder holder NN 10_1101-2021_01_07_425794 208 6 for for IN 10_1101-2021_01_07_425794 208 7 this this DT 10_1101-2021_01_07_425794 208 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 208 9 version version NN 10_1101-2021_01_07_425794 208 10 posted post VBD 10_1101-2021_01_07_425794 208 11 January January NNP 10_1101-2021_01_07_425794 208 12 8 8 CD 10_1101-2021_01_07_425794 208 13 , , , 10_1101-2021_01_07_425794 208 14 2021 2021 CD 10_1101-2021_01_07_425794 208 15 . . . 10_1101-2021_01_07_425794 208 16 ; ; : 10_1101-2021_01_07_425794 208 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 208 18 : : : 10_1101-2021_01_07_425794 208 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 208 20 preprint preprint JJ 10_1101-2021_01_07_425794 208 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 208 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425794 208 23 Key Key NNP 10_1101-2021_01_07_425794 208 24 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 208 25 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 208 26 - - HYPH 10_1101-2021_01_07_425794 208 27 NCBI NCBI NNP 10_1101-2021_01_07_425794 208 28 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 208 29 - - HYPH 10_1101-2021_01_07_425794 208 30 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 208 31 All all DT 10_1101-2021_01_07_425794 208 32 genes gene NNS 10_1101-2021_01_07_425794 208 33 after after IN 10_1101-2021_01_07_425794 208 34 filtering filter VBG 10_1101-2021_01_07_425794 208 35 Common common JJ 10_1101-2021_01_07_425794 208 36 genes gene NNS 10_1101-2021_01_07_425794 208 37 after after IN 10_1101-2021_01_07_425794 208 38 filtering filter VBG 10_1101-2021_01_07_425794 208 39 0.70 0.70 CD 10_1101-2021_01_07_425794 208 40 0.75 0.75 CD 10_1101-2021_01_07_425794 208 41 0.80 0.80 CD 10_1101-2021_01_07_425794 208 42 0.85 0.85 CD 10_1101-2021_01_07_425794 208 43 0.70 0.70 CD 10_1101-2021_01_07_425794 208 44 0.75 0.75 CD 10_1101-2021_01_07_425794 208 45 0.80 0.80 CD 10_1101-2021_01_07_425794 208 46 0.85 0.85 CD 10_1101-2021_01_07_425794 208 47 A A NNP 10_1101-2021_01_07_425794 208 48 B b NN 10_1101-2021_01_07_425794 208 49 C c NN 10_1101-2021_01_07_425794 208 50 D d NN 10_1101-2021_01_07_425794 208 51 A a NN 10_1101-2021_01_07_425794 208 52 B b NN 10_1101-2021_01_07_425794 208 53 C c NN 10_1101-2021_01_07_425794 208 54 D d NN 10_1101-2021_01_07_425794 208 55 0.70 0.70 CD 10_1101-2021_01_07_425794 208 56 0.75 0.75 CD 10_1101-2021_01_07_425794 208 57 0.80 0.80 CD 10_1101-2021_01_07_425794 208 58 0.85 0.85 CD 10_1101-2021_01_07_425794 208 59 Li Li NNP 10_1101-2021_01_07_425794 208 60 br br IN 10_1101-2021_01_07_425794 208 61 ar ar NNP 10_1101-2021_01_07_425794 208 62 y y NNP 10_1101-2021_01_07_425794 208 63 si si NN 10_1101-2021_01_07_425794 208 64 ze ze UH 10_1101-2021_01_07_425794 208 65 no no UH 10_1101-2021_01_07_425794 208 66 rm rm NNP 10_1101-2021_01_07_425794 208 67 al al NNP 10_1101-2021_01_07_425794 208 68 iz iz NNP 10_1101-2021_01_07_425794 208 69 at at IN 10_1101-2021_01_07_425794 208 70 io io NNP 10_1101-2021_01_07_425794 208 71 n n NNP 10_1101-2021_01_07_425794 208 72 Q Q NNP 10_1101-2021_01_07_425794 208 73 ua ua PRP 10_1101-2021_01_07_425794 208 74 nt nt RB 10_1101-2021_01_07_425794 208 75 ile ile VB 10_1101-2021_01_07_425794 208 76 no no UH 10_1101-2021_01_07_425794 208 77 rm rm NNP 10_1101-2021_01_07_425794 208 78 al al NNP 10_1101-2021_01_07_425794 208 79 iz iz NNP 10_1101-2021_01_07_425794 208 80 at at IN 10_1101-2021_01_07_425794 208 81 io io NNP 10_1101-2021_01_07_425794 208 82 n n CC 10_1101-2021_01_07_425794 208 83 TM TM NNP 10_1101-2021_01_07_425794 208 84 M M NNP 10_1101-2021_01_07_425794 208 85 no no UH 10_1101-2021_01_07_425794 208 86 rm rm NNP 10_1101-2021_01_07_425794 208 87 al al NNP 10_1101-2021_01_07_425794 208 88 iz iz NNP 10_1101-2021_01_07_425794 208 89 at at IN 10_1101-2021_01_07_425794 208 90 io io NNP 10_1101-2021_01_07_425794 208 91 n n CC 10_1101-2021_01_07_425794 208 92 0.70 0.70 CD 10_1101-2021_01_07_425794 208 93 0.75 0.75 CD 10_1101-2021_01_07_425794 208 94 0.80 0.80 CD 10_1101-2021_01_07_425794 208 95 0.85 0.85 CD 10_1101-2021_01_07_425794 208 96 0.70 0.70 CD 10_1101-2021_01_07_425794 208 97 0.75 0.75 CD 10_1101-2021_01_07_425794 208 98 0.80 0.80 CD 10_1101-2021_01_07_425794 208 99 0.85 0.85 CD 10_1101-2021_01_07_425794 208 100 0.70 0.70 CD 10_1101-2021_01_07_425794 208 101 0.75 0.75 CD 10_1101-2021_01_07_425794 208 102 0.80 0.80 CD 10_1101-2021_01_07_425794 208 103 0.85 0.85 CD 10_1101-2021_01_07_425794 208 104 C C NNP 10_1101-2021_01_07_425794 208 105 or or CC 10_1101-2021_01_07_425794 208 106 re re VB 10_1101-2021_01_07_425794 208 107 la la NNP 10_1101-2021_01_07_425794 208 108 tio tio NNP 10_1101-2021_01_07_425794 208 109 n n NNP 10_1101-2021_01_07_425794 208 110 co co NNP 10_1101-2021_01_07_425794 208 111 ef ef NNP 10_1101-2021_01_07_425794 208 112 fic fic NNP 10_1101-2021_01_07_425794 208 113 ie ie NNP 10_1101-2021_01_07_425794 208 114 nt nt RB 10_1101-2021_01_07_425794 208 115 C C NNP 10_1101-2021_01_07_425794 208 116 or or CC 10_1101-2021_01_07_425794 208 117 re re VB 10_1101-2021_01_07_425794 208 118 la la NNP 10_1101-2021_01_07_425794 208 119 tio tio NNP 10_1101-2021_01_07_425794 208 120 n n NNP 10_1101-2021_01_07_425794 208 121 co co NNP 10_1101-2021_01_07_425794 208 122 ef ef NNP 10_1101-2021_01_07_425794 208 123 fic fic NNP 10_1101-2021_01_07_425794 208 124 ie ie NNP 10_1101-2021_01_07_425794 208 125 nt nt RB 10_1101-2021_01_07_425794 208 126 C C NNP 10_1101-2021_01_07_425794 208 127 or or CC 10_1101-2021_01_07_425794 208 128 re re VB 10_1101-2021_01_07_425794 208 129 la la NNP 10_1101-2021_01_07_425794 208 130 tio tio NNP 10_1101-2021_01_07_425794 208 131 n n NNP 10_1101-2021_01_07_425794 208 132 co co NNP 10_1101-2021_01_07_425794 208 133 ef ef NNP 10_1101-2021_01_07_425794 208 134 fic fic NNP 10_1101-2021_01_07_425794 208 135 ie ie NNP 10_1101-2021_01_07_425794 208 136 nt nt NNP 10_1101-2021_01_07_425794 208 137 Figure Figure NNP 10_1101-2021_01_07_425794 208 138 6 6 CD 10_1101-2021_01_07_425794 208 139 : : : 10_1101-2021_01_07_425794 208 140 Validation validation NN 10_1101-2021_01_07_425794 208 141 of of IN 10_1101-2021_01_07_425794 208 142 RNA RNA NNP 10_1101-2021_01_07_425794 208 143 - - HYPH 10_1101-2021_01_07_425794 208 144 seq seq NN 10_1101-2021_01_07_425794 208 145 against against IN 10_1101-2021_01_07_425794 208 146 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 208 147 RT RT NNP 10_1101-2021_01_07_425794 208 148 - - HYPH 10_1101-2021_01_07_425794 208 149 PCR PCR NNP 10_1101-2021_01_07_425794 208 150 dataset dataset NN 10_1101-2021_01_07_425794 208 151 . . . 10_1101-2021_01_07_425794 209 1 Shown show VBN 10_1101-2021_01_07_425794 209 2 are be VBP 10_1101-2021_01_07_425794 209 3 Pearson Pearson NNP 10_1101-2021_01_07_425794 209 4 correlation correlation NN 10_1101-2021_01_07_425794 209 5 coefficients coefficient NNS 10_1101-2021_01_07_425794 209 6 computed compute VBD 10_1101-2021_01_07_425794 209 7 from from IN 10_1101-2021_01_07_425794 209 8 comparing compare VBG 10_1101-2021_01_07_425794 209 9 RNA RNA NNP 10_1101-2021_01_07_425794 209 10 - - HYPH 10_1101-2021_01_07_425794 209 11 seq seq NN 10_1101-2021_01_07_425794 209 12 data datum NNS 10_1101-2021_01_07_425794 209 13 against against IN 10_1101-2021_01_07_425794 209 14 RT RT NNP 10_1101-2021_01_07_425794 209 15 - - HYPH 10_1101-2021_01_07_425794 209 16 PCR PCR NNP 10_1101-2021_01_07_425794 209 17 data datum NNS 10_1101-2021_01_07_425794 209 18 , , , 10_1101-2021_01_07_425794 209 19 using use VBG 10_1101-2021_01_07_425794 209 20 the the DT 10_1101-2021_01_07_425794 209 21 RT RT NNP 10_1101-2021_01_07_425794 209 22 - - HYPH 10_1101-2021_01_07_425794 209 23 PCR PCR NNP 10_1101-2021_01_07_425794 209 24 genes gene NNS 10_1101-2021_01_07_425794 209 25 matched match VBN 10_1101-2021_01_07_425794 209 26 with with IN 10_1101-2021_01_07_425794 209 27 each each DT 10_1101-2021_01_07_425794 209 28 individual individual JJ 10_1101-2021_01_07_425794 209 29 annotation annotation NN 10_1101-2021_01_07_425794 209 30 ( ( -LRB- 10_1101-2021_01_07_425794 209 31 left leave VBN 10_1101-2021_01_07_425794 209 32 column column NN 10_1101-2021_01_07_425794 209 33 ) ) -RRB- 10_1101-2021_01_07_425794 209 34 or or CC 10_1101-2021_01_07_425794 209 35 matched match VBN 10_1101-2021_01_07_425794 209 36 with with IN 10_1101-2021_01_07_425794 209 37 all all DT 10_1101-2021_01_07_425794 209 38 three three CD 10_1101-2021_01_07_425794 209 39 annotations annotation NNS 10_1101-2021_01_07_425794 209 40 ( ( -LRB- 10_1101-2021_01_07_425794 209 41 right right JJ 10_1101-2021_01_07_425794 209 42 column column NN 10_1101-2021_01_07_425794 209 43 ) ) -RRB- 10_1101-2021_01_07_425794 209 44 . . . 10_1101-2021_01_07_425794 210 1 The the DT 10_1101-2021_01_07_425794 210 2 rows row NNS 10_1101-2021_01_07_425794 210 3 represent represent VBP 10_1101-2021_01_07_425794 210 4 the the DT 10_1101-2021_01_07_425794 210 5 different different JJ 10_1101-2021_01_07_425794 210 6 RNA RNA NNP 10_1101-2021_01_07_425794 210 7 - - HYPH 10_1101-2021_01_07_425794 210 8 seq seq NN 10_1101-2021_01_07_425794 210 9 normalization normalization NN 10_1101-2021_01_07_425794 210 10 methods method NNS 10_1101-2021_01_07_425794 210 11 used use VBN 10_1101-2021_01_07_425794 210 12 . . . 10_1101-2021_01_07_425794 211 1 Lowly Lowly NNP 10_1101-2021_01_07_425794 211 2 expressed express VBD 10_1101-2021_01_07_425794 211 3 genes gene NNS 10_1101-2021_01_07_425794 211 4 in in IN 10_1101-2021_01_07_425794 211 5 the the DT 10_1101-2021_01_07_425794 211 6 RNA RNA NNP 10_1101-2021_01_07_425794 211 7 - - HYPH 10_1101-2021_01_07_425794 211 8 seq seq NN 10_1101-2021_01_07_425794 211 9 data datum NNS 10_1101-2021_01_07_425794 211 10 were be VBD 10_1101-2021_01_07_425794 211 11 filtered filter VBN 10_1101-2021_01_07_425794 211 12 out out RP 10_1101-2021_01_07_425794 211 13 before before IN 10_1101-2021_01_07_425794 211 14 the the DT 10_1101-2021_01_07_425794 211 15 correlation correlation NN 10_1101-2021_01_07_425794 211 16 analysis analysis NN 10_1101-2021_01_07_425794 211 17 was be VBD 10_1101-2021_01_07_425794 211 18 performed perform VBN 10_1101-2021_01_07_425794 211 19 . . . 10_1101-2021_01_07_425794 212 1 The the DT 10_1101-2021_01_07_425794 212 2 RNA RNA NNP 10_1101-2021_01_07_425794 212 3 - - HYPH 10_1101-2021_01_07_425794 212 4 seq seq NN 10_1101-2021_01_07_425794 212 5 data datum NNS 10_1101-2021_01_07_425794 212 6 generated generate VBN 10_1101-2021_01_07_425794 212 7 from from IN 10_1101-2021_01_07_425794 212 8 each each DT 10_1101-2021_01_07_425794 212 9 annotation annotation NN 10_1101-2021_01_07_425794 212 10 were be VBD 10_1101-2021_01_07_425794 212 11 filtered filter VBN 10_1101-2021_01_07_425794 212 12 to to TO 10_1101-2021_01_07_425794 212 13 remove remove VB 10_1101-2021_01_07_425794 212 14 lowly lowly RB 10_1101-2021_01_07_425794 212 15 expressed express VBN 10_1101-2021_01_07_425794 212 16 genes gene NNS 10_1101-2021_01_07_425794 212 17 before before IN 10_1101-2021_01_07_425794 212 18 being be VBG 10_1101-2021_01_07_425794 212 19 compared compare VBN 10_1101-2021_01_07_425794 212 20 to to IN 10_1101-2021_01_07_425794 212 21 the the DT 10_1101-2021_01_07_425794 212 22 RT RT NNP 10_1101-2021_01_07_425794 212 23 - - HYPH 10_1101-2021_01_07_425794 212 24 PCR PCR NNP 10_1101-2021_01_07_425794 212 25 data datum NNS 10_1101-2021_01_07_425794 212 26 . . . 10_1101-2021_01_07_425794 213 1 Numbers number NNS 10_1101-2021_01_07_425794 213 2 of of IN 10_1101-2021_01_07_425794 213 3 matched match VBN 10_1101-2021_01_07_425794 213 4 genes gene NNS 10_1101-2021_01_07_425794 213 5 between between IN 10_1101-2021_01_07_425794 213 6 the the DT 10_1101-2021_01_07_425794 213 7 RT RT NNP 10_1101-2021_01_07_425794 213 8 - - HYPH 10_1101-2021_01_07_425794 213 9 PCR PCR NNP 10_1101-2021_01_07_425794 213 10 data datum NNS 10_1101-2021_01_07_425794 213 11 and and CC 10_1101-2021_01_07_425794 213 12 the the DT 10_1101-2021_01_07_425794 213 13 RNA RNA NNP 10_1101-2021_01_07_425794 213 14 - - HYPH 10_1101-2021_01_07_425794 213 15 seq seq NN 10_1101-2021_01_07_425794 213 16 data datum NNS 10_1101-2021_01_07_425794 213 17 were be VBD 10_1101-2021_01_07_425794 213 18 856 856 CD 10_1101-2021_01_07_425794 213 19 , , , 10_1101-2021_01_07_425794 213 20 901 901 CD 10_1101-2021_01_07_425794 213 21 and and CC 10_1101-2021_01_07_425794 213 22 901 901 CD 10_1101-2021_01_07_425794 213 23 for for IN 10_1101-2021_01_07_425794 213 24 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 213 25 , , , 10_1101-2021_01_07_425794 213 26 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 213 27 - - HYPH 10_1101-2021_01_07_425794 213 28 NCBI NCBI NNP 10_1101-2021_01_07_425794 213 29 and and CC 10_1101-2021_01_07_425794 213 30 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 213 31 - - HYPH 10_1101-2021_01_07_425794 213 32 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 213 33 , , , 10_1101-2021_01_07_425794 213 34 respectively respectively RB 10_1101-2021_01_07_425794 213 35 . . . 10_1101-2021_01_07_425794 214 1 846 846 CD 10_1101-2021_01_07_425794 214 2 RT RT NNP 10_1101-2021_01_07_425794 214 3 - - HYPH 10_1101-2021_01_07_425794 214 4 PCR PCR NNP 10_1101-2021_01_07_425794 214 5 genes gene NNS 10_1101-2021_01_07_425794 214 6 were be VBD 10_1101-2021_01_07_425794 214 7 found find VBN 10_1101-2021_01_07_425794 214 8 to to TO 10_1101-2021_01_07_425794 214 9 be be VB 10_1101-2021_01_07_425794 214 10 common common JJ 10_1101-2021_01_07_425794 214 11 to to IN 10_1101-2021_01_07_425794 214 12 all all PDT 10_1101-2021_01_07_425794 214 13 the the DT 10_1101-2021_01_07_425794 214 14 three three CD 10_1101-2021_01_07_425794 214 15 annotations annotation NNS 10_1101-2021_01_07_425794 214 16 . . . 10_1101-2021_01_07_425794 215 1 The the DT 10_1101-2021_01_07_425794 215 2 raw raw JJ 10_1101-2021_01_07_425794 215 3 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 215 4 RT RT NNP 10_1101-2021_01_07_425794 215 5 - - HYPH 10_1101-2021_01_07_425794 215 6 PCR PCR NNP 10_1101-2021_01_07_425794 215 7 data datum NNS 10_1101-2021_01_07_425794 215 8 were be VBD 10_1101-2021_01_07_425794 215 9 log2- log2- CD 10_1101-2021_01_07_425794 215 10 16 16 CD 10_1101-2021_01_07_425794 215 11 .CC .CC : 10_1101-2021_01_07_425794 215 12 - - HYPH 10_1101-2021_01_07_425794 215 13 BY by IN 10_1101-2021_01_07_425794 215 14 - - HYPH 10_1101-2021_01_07_425794 215 15 NC NC NNP 10_1101-2021_01_07_425794 215 16 - - HYPH 10_1101-2021_01_07_425794 215 17 ND ND NNP 10_1101-2021_01_07_425794 215 18 4.0 4.0 CD 10_1101-2021_01_07_425794 215 19 International international JJ 10_1101-2021_01_07_425794 215 20 licensemade licensemade NN 10_1101-2021_01_07_425794 215 21 available available JJ 10_1101-2021_01_07_425794 215 22 under under IN 10_1101-2021_01_07_425794 215 23 a a DT 10_1101-2021_01_07_425794 215 24 ( ( -LRB- 10_1101-2021_01_07_425794 215 25 which which WDT 10_1101-2021_01_07_425794 215 26 was be VBD 10_1101-2021_01_07_425794 215 27 not not RB 10_1101-2021_01_07_425794 215 28 certified certify VBN 10_1101-2021_01_07_425794 215 29 by by IN 10_1101-2021_01_07_425794 215 30 peer peer NN 10_1101-2021_01_07_425794 215 31 review review NN 10_1101-2021_01_07_425794 215 32 ) ) -RRB- 10_1101-2021_01_07_425794 215 33 is be VBZ 10_1101-2021_01_07_425794 215 34 the the DT 10_1101-2021_01_07_425794 215 35 author author NN 10_1101-2021_01_07_425794 215 36 / / SYM 10_1101-2021_01_07_425794 215 37 funder funder NN 10_1101-2021_01_07_425794 215 38 , , , 10_1101-2021_01_07_425794 215 39 who who WP 10_1101-2021_01_07_425794 215 40 has have VBZ 10_1101-2021_01_07_425794 215 41 granted grant VBN 10_1101-2021_01_07_425794 215 42 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 215 43 a a DT 10_1101-2021_01_07_425794 215 44 license license NN 10_1101-2021_01_07_425794 215 45 to to TO 10_1101-2021_01_07_425794 215 46 display display VB 10_1101-2021_01_07_425794 215 47 the the DT 10_1101-2021_01_07_425794 215 48 preprint preprint NN 10_1101-2021_01_07_425794 215 49 in in IN 10_1101-2021_01_07_425794 215 50 perpetuity perpetuity NN 10_1101-2021_01_07_425794 215 51 . . . 10_1101-2021_01_07_425794 216 1 It -PRON- PRP 10_1101-2021_01_07_425794 216 2 is be VBZ 10_1101-2021_01_07_425794 216 3 The the DT 10_1101-2021_01_07_425794 216 4 copyright copyright NN 10_1101-2021_01_07_425794 216 5 holder holder NN 10_1101-2021_01_07_425794 216 6 for for IN 10_1101-2021_01_07_425794 216 7 this this DT 10_1101-2021_01_07_425794 216 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 216 9 version version NN 10_1101-2021_01_07_425794 216 10 posted post VBD 10_1101-2021_01_07_425794 216 11 January January NNP 10_1101-2021_01_07_425794 216 12 8 8 CD 10_1101-2021_01_07_425794 216 13 , , , 10_1101-2021_01_07_425794 216 14 2021 2021 CD 10_1101-2021_01_07_425794 216 15 . . . 10_1101-2021_01_07_425794 216 16 ; ; : 10_1101-2021_01_07_425794 216 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 216 18 : : : 10_1101-2021_01_07_425794 216 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 216 20 preprint preprint JJ 10_1101-2021_01_07_425794 216 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 216 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 216 23 transformed transform VBN 10_1101-2021_01_07_425794 216 24 before before IN 10_1101-2021_01_07_425794 216 25 comparing compare VBG 10_1101-2021_01_07_425794 216 26 to to IN 10_1101-2021_01_07_425794 216 27 the the DT 10_1101-2021_01_07_425794 216 28 filtered filter VBN 10_1101-2021_01_07_425794 216 29 RNA RNA NNP 10_1101-2021_01_07_425794 216 30 - - HYPH 10_1101-2021_01_07_425794 216 31 seq seq NN 10_1101-2021_01_07_425794 216 32 data datum NNS 10_1101-2021_01_07_425794 216 33 . . . 10_1101-2021_01_07_425794 217 1 Pearson Pearson NNP 10_1101-2021_01_07_425794 217 2 correlation correlation NN 10_1101-2021_01_07_425794 217 3 analysis analysis NN 10_1101-2021_01_07_425794 217 4 of of IN 10_1101-2021_01_07_425794 217 5 the the DT 10_1101-2021_01_07_425794 217 6 RNA RNA NNP 10_1101-2021_01_07_425794 217 7 - - HYPH 10_1101-2021_01_07_425794 217 8 seq seq NN 10_1101-2021_01_07_425794 217 9 gene gene NN 10_1101-2021_01_07_425794 217 10 expression expression NN 10_1101-2021_01_07_425794 217 11 ( ( -LRB- 10_1101-2021_01_07_425794 217 12 log2FPKM log2fpkm CD 10_1101-2021_01_07_425794 217 13 values value NNS 10_1101-2021_01_07_425794 217 14 ) ) -RRB- 10_1101-2021_01_07_425794 217 15 and and CC 10_1101-2021_01_07_425794 217 16 RT RT NNP 10_1101-2021_01_07_425794 217 17 - - HYPH 10_1101-2021_01_07_425794 217 18 PCR PCR NNP 10_1101-2021_01_07_425794 217 19 gene gene NN 10_1101-2021_01_07_425794 217 20 expression expression NN 10_1101-2021_01_07_425794 217 21 ( ( -LRB- 10_1101-2021_01_07_425794 217 22 log2 log2 NN 10_1101-2021_01_07_425794 217 23 values value NNS 10_1101-2021_01_07_425794 217 24 ) ) -RRB- 10_1101-2021_01_07_425794 217 25 from from IN 10_1101-2021_01_07_425794 217 26 using use VBG 10_1101-2021_01_07_425794 217 27 the the DT 10_1101-2021_01_07_425794 217 28 RT RT NNP 10_1101-2021_01_07_425794 217 29 - - HYPH 10_1101-2021_01_07_425794 217 30 PCR PCR NNP 10_1101-2021_01_07_425794 217 31 genes gene NNS 10_1101-2021_01_07_425794 217 32 matched match VBN 10_1101-2021_01_07_425794 217 33 with with IN 10_1101-2021_01_07_425794 217 34 each each DT 10_1101-2021_01_07_425794 217 35 individual individual JJ 10_1101-2021_01_07_425794 217 36 annotation annotation NN 10_1101-2021_01_07_425794 217 37 showed show VBD 10_1101-2021_01_07_425794 217 38 that that IN 10_1101-2021_01_07_425794 217 39 the the DT 10_1101-2021_01_07_425794 217 40 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 217 41 - - HYPH 10_1101-2021_01_07_425794 217 42 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 217 43 annotation annotation NN 10_1101-2021_01_07_425794 217 44 constantly constantly RB 10_1101-2021_01_07_425794 217 45 yielded yield VBD 10_1101-2021_01_07_425794 217 46 a a DT 10_1101-2021_01_07_425794 217 47 higher high JJR 10_1101-2021_01_07_425794 217 48 correlation correlation NN 10_1101-2021_01_07_425794 217 49 than than IN 10_1101-2021_01_07_425794 217 50 the the DT 10_1101-2021_01_07_425794 217 51 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 217 52 and and CC 10_1101-2021_01_07_425794 217 53 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 217 54 - - HYPH 10_1101-2021_01_07_425794 217 55 NCBI NCBI NNP 10_1101-2021_01_07_425794 217 56 annotations annotation NNS 10_1101-2021_01_07_425794 217 57 , , , 10_1101-2021_01_07_425794 217 58 across across IN 10_1101-2021_01_07_425794 217 59 all all PDT 10_1101-2021_01_07_425794 217 60 the the DT 10_1101-2021_01_07_425794 217 61 samples sample NNS 10_1101-2021_01_07_425794 217 62 and and CC 10_1101-2021_01_07_425794 217 63 the the DT 10_1101-2021_01_07_425794 217 64 three three CD 10_1101-2021_01_07_425794 217 65 different different JJ 10_1101-2021_01_07_425794 217 66 normalization normalization NN 10_1101-2021_01_07_425794 217 67 methods method NNS 10_1101-2021_01_07_425794 217 68 ( ( -LRB- 10_1101-2021_01_07_425794 217 69 left left JJ 10_1101-2021_01_07_425794 217 70 panel panel NN 10_1101-2021_01_07_425794 217 71 in in IN 10_1101-2021_01_07_425794 217 72 Figure Figure NNP 10_1101-2021_01_07_425794 217 73 6 6 CD 10_1101-2021_01_07_425794 217 74 ) ) -RRB- 10_1101-2021_01_07_425794 217 75 . . . 10_1101-2021_01_07_425794 218 1 The the DT 10_1101-2021_01_07_425794 218 2 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 218 3 annotation annotation NN 10_1101-2021_01_07_425794 218 4 was be VBD 10_1101-2021_01_07_425794 218 5 found find VBN 10_1101-2021_01_07_425794 218 6 to to TO 10_1101-2021_01_07_425794 218 7 produce produce VB 10_1101-2021_01_07_425794 218 8 the the DT 10_1101-2021_01_07_425794 218 9 worst bad JJS 10_1101-2021_01_07_425794 218 10 correlation correlation NN 10_1101-2021_01_07_425794 218 11 in in IN 10_1101-2021_01_07_425794 218 12 all all PDT 10_1101-2021_01_07_425794 218 13 these these DT 10_1101-2021_01_07_425794 218 14 comparisons comparison NNS 10_1101-2021_01_07_425794 218 15 . . . 10_1101-2021_01_07_425794 219 1 When when WRB 10_1101-2021_01_07_425794 219 2 using use VBG 10_1101-2021_01_07_425794 219 3 the the DT 10_1101-2021_01_07_425794 219 4 RT RT NNP 10_1101-2021_01_07_425794 219 5 - - HYPH 10_1101-2021_01_07_425794 219 6 PCR PCR NNP 10_1101-2021_01_07_425794 219 7 genes gene NNS 10_1101-2021_01_07_425794 219 8 matched match VBN 10_1101-2021_01_07_425794 219 9 with with IN 10_1101-2021_01_07_425794 219 10 all all DT 10_1101-2021_01_07_425794 219 11 three three CD 10_1101-2021_01_07_425794 219 12 annotations annotation NNS 10_1101-2021_01_07_425794 219 13 for for IN 10_1101-2021_01_07_425794 219 14 comparison comparison NN 10_1101-2021_01_07_425794 219 15 , , , 10_1101-2021_01_07_425794 219 16 RefSeq- RefSeq- NNP 10_1101-2021_01_07_425794 219 17 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 219 18 was be VBD 10_1101-2021_01_07_425794 219 19 again again RB 10_1101-2021_01_07_425794 219 20 found find VBN 10_1101-2021_01_07_425794 219 21 to to TO 10_1101-2021_01_07_425794 219 22 yield yield VB 10_1101-2021_01_07_425794 219 23 the the DT 10_1101-2021_01_07_425794 219 24 highest high JJS 10_1101-2021_01_07_425794 219 25 correlation correlation NN 10_1101-2021_01_07_425794 219 26 ( ( -LRB- 10_1101-2021_01_07_425794 219 27 right right JJ 10_1101-2021_01_07_425794 219 28 panel panel NN 10_1101-2021_01_07_425794 219 29 in in IN 10_1101-2021_01_07_425794 219 30 Figure Figure NNP 10_1101-2021_01_07_425794 219 31 6 6 CD 10_1101-2021_01_07_425794 219 32 ) ) -RRB- 10_1101-2021_01_07_425794 219 33 . . . 10_1101-2021_01_07_425794 220 1 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 220 2 and and CC 10_1101-2021_01_07_425794 220 3 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 220 4 - - HYPH 10_1101-2021_01_07_425794 220 5 NCBI NCBI NNP 10_1101-2021_01_07_425794 220 6 were be VBD 10_1101-2021_01_07_425794 220 7 found find VBN 10_1101-2021_01_07_425794 220 8 to to TO 10_1101-2021_01_07_425794 220 9 produce produce VB 10_1101-2021_01_07_425794 220 10 similar similar JJ 10_1101-2021_01_07_425794 220 11 correlation correlation NN 10_1101-2021_01_07_425794 220 12 coefficients coefficient NNS 10_1101-2021_01_07_425794 220 13 . . . 10_1101-2021_01_07_425794 221 1 Taken take VBN 10_1101-2021_01_07_425794 221 2 together together RB 10_1101-2021_01_07_425794 221 3 , , , 10_1101-2021_01_07_425794 221 4 results result NNS 10_1101-2021_01_07_425794 221 5 from from IN 10_1101-2021_01_07_425794 221 6 this this DT 10_1101-2021_01_07_425794 221 7 evaluation evaluation NN 10_1101-2021_01_07_425794 221 8 showed show VBD 10_1101-2021_01_07_425794 221 9 that that IN 10_1101-2021_01_07_425794 221 10 the the DT 10_1101-2021_01_07_425794 221 11 use use NN 10_1101-2021_01_07_425794 221 12 of of IN 10_1101-2021_01_07_425794 221 13 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 221 14 - - HYPH 10_1101-2021_01_07_425794 221 15 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 221 16 annotation annotation NN 10_1101-2021_01_07_425794 221 17 led lead VBD 10_1101-2021_01_07_425794 221 18 to to IN 10_1101-2021_01_07_425794 221 19 a a DT 10_1101-2021_01_07_425794 221 20 better well JJR 10_1101-2021_01_07_425794 221 21 concordance concordance NN 10_1101-2021_01_07_425794 221 22 in in IN 10_1101-2021_01_07_425794 221 23 gene gene NN 10_1101-2021_01_07_425794 221 24 expression expression NN 10_1101-2021_01_07_425794 221 25 between between IN 10_1101-2021_01_07_425794 221 26 the the DT 10_1101-2021_01_07_425794 221 27 RNA RNA NNP 10_1101-2021_01_07_425794 221 28 - - HYPH 10_1101-2021_01_07_425794 221 29 seq seq NN 10_1101-2021_01_07_425794 221 30 data datum NNS 10_1101-2021_01_07_425794 221 31 and and CC 10_1101-2021_01_07_425794 221 32 the the DT 10_1101-2021_01_07_425794 221 33 RT- RT- NNP 10_1101-2021_01_07_425794 221 34 PCR PCR NNP 10_1101-2021_01_07_425794 221 35 data datum NNS 10_1101-2021_01_07_425794 221 36 , , , 10_1101-2021_01_07_425794 221 37 compared compare VBN 10_1101-2021_01_07_425794 221 38 to to IN 10_1101-2021_01_07_425794 221 39 the the DT 10_1101-2021_01_07_425794 221 40 use use NN 10_1101-2021_01_07_425794 221 41 of of IN 10_1101-2021_01_07_425794 221 42 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 221 43 and and CC 10_1101-2021_01_07_425794 221 44 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 221 45 - - HYPH 10_1101-2021_01_07_425794 221 46 NCBI NCBI NNP 10_1101-2021_01_07_425794 221 47 annotations annotation NNS 10_1101-2021_01_07_425794 221 48 . . . 10_1101-2021_01_07_425794 222 1 3.7 3.7 CD 10_1101-2021_01_07_425794 222 2 Validation validation NN 10_1101-2021_01_07_425794 222 3 against against IN 10_1101-2021_01_07_425794 222 4 microarray microarray JJ 10_1101-2021_01_07_425794 222 5 data datum NNS 10_1101-2021_01_07_425794 222 6 An an DT 10_1101-2021_01_07_425794 222 7 Illumina Illumina NNP 10_1101-2021_01_07_425794 222 8 BeadChip BeadChip NNP 10_1101-2021_01_07_425794 222 9 microarray microarray NN 10_1101-2021_01_07_425794 222 10 dataset dataset NN 10_1101-2021_01_07_425794 222 11 , , , 10_1101-2021_01_07_425794 222 12 which which WDT 10_1101-2021_01_07_425794 222 13 was be VBD 10_1101-2021_01_07_425794 222 14 generated generate VBN 10_1101-2021_01_07_425794 222 15 by by IN 10_1101-2021_01_07_425794 222 16 the the DT 10_1101-2021_01_07_425794 222 17 MAQC MAQC NNP 10_1101-2021_01_07_425794 222 18 - - : 10_1101-2021_01_07_425794 222 19 I -PRON- PRP 10_1101-2021_01_07_425794 222 20 project project VBP 10_1101-2021_01_07_425794 222 21 [ [ -LRB- 10_1101-2021_01_07_425794 222 22 15 15 CD 10_1101-2021_01_07_425794 222 23 ] ] -RRB- 10_1101-2021_01_07_425794 222 24 for for IN 10_1101-2021_01_07_425794 222 25 the the DT 10_1101-2021_01_07_425794 222 26 same same JJ 10_1101-2021_01_07_425794 222 27 samples sample NNS 10_1101-2021_01_07_425794 222 28 as as IN 10_1101-2021_01_07_425794 222 29 in in IN 10_1101-2021_01_07_425794 222 30 the the DT 10_1101-2021_01_07_425794 222 31 RNA RNA NNP 10_1101-2021_01_07_425794 222 32 - - HYPH 10_1101-2021_01_07_425794 222 33 seq seq NN 10_1101-2021_01_07_425794 222 34 data datum NNS 10_1101-2021_01_07_425794 222 35 used use VBN 10_1101-2021_01_07_425794 222 36 in in IN 10_1101-2021_01_07_425794 222 37 this this DT 10_1101-2021_01_07_425794 222 38 study study NN 10_1101-2021_01_07_425794 222 39 , , , 10_1101-2021_01_07_425794 222 40 was be VBD 10_1101-2021_01_07_425794 222 41 used use VBN 10_1101-2021_01_07_425794 222 42 to to TO 10_1101-2021_01_07_425794 222 43 further further RB 10_1101-2021_01_07_425794 222 44 validate validate VB 10_1101-2021_01_07_425794 222 45 the the DT 10_1101-2021_01_07_425794 222 46 gene gene NN 10_1101-2021_01_07_425794 222 47 - - HYPH 10_1101-2021_01_07_425794 222 48 level level NN 10_1101-2021_01_07_425794 222 49 RNA RNA NNP 10_1101-2021_01_07_425794 222 50 - - HYPH 10_1101-2021_01_07_425794 222 51 seq seq NN 10_1101-2021_01_07_425794 222 52 quantification quantification NN 10_1101-2021_01_07_425794 222 53 results result NNS 10_1101-2021_01_07_425794 222 54 obtained obtain VBN 10_1101-2021_01_07_425794 222 55 from from IN 10_1101-2021_01_07_425794 222 56 different different JJ 10_1101-2021_01_07_425794 222 57 annota- annota- JJ 10_1101-2021_01_07_425794 222 58 tions tion NNS 10_1101-2021_01_07_425794 222 59 . . . 10_1101-2021_01_07_425794 223 1 The the DT 10_1101-2021_01_07_425794 223 2 microarray microarray JJ 10_1101-2021_01_07_425794 223 3 dataset dataset NN 10_1101-2021_01_07_425794 223 4 was be VBD 10_1101-2021_01_07_425794 223 5 background background NN 10_1101-2021_01_07_425794 223 6 corrected correct VBN 10_1101-2021_01_07_425794 223 7 and and CC 10_1101-2021_01_07_425794 223 8 normalized normalize VBD 10_1101-2021_01_07_425794 223 9 using use VBG 10_1101-2021_01_07_425794 223 10 the the DT 10_1101-2021_01_07_425794 223 11 ‘ ' `` 10_1101-2021_01_07_425794 223 12 neqc neqc NN 10_1101-2021_01_07_425794 223 13 ’ ' '' 10_1101-2021_01_07_425794 223 14 function function NN 10_1101-2021_01_07_425794 223 15 in in IN 10_1101-2021_01_07_425794 223 16 the the DT 10_1101-2021_01_07_425794 223 17 limma limma NN 10_1101-2021_01_07_425794 223 18 package package NN 10_1101-2021_01_07_425794 223 19 [ [ -LRB- 10_1101-2021_01_07_425794 223 20 26 26 CD 10_1101-2021_01_07_425794 223 21 , , , 10_1101-2021_01_07_425794 223 22 18 18 CD 10_1101-2021_01_07_425794 223 23 ] ] -RRB- 10_1101-2021_01_07_425794 223 24 . . . 10_1101-2021_01_07_425794 224 1 Microarray microarray JJ 10_1101-2021_01_07_425794 224 2 genes gene NNS 10_1101-2021_01_07_425794 224 3 were be VBD 10_1101-2021_01_07_425794 224 4 then then RB 10_1101-2021_01_07_425794 224 5 matched match VBN 10_1101-2021_01_07_425794 224 6 to to IN 10_1101-2021_01_07_425794 224 7 the the DT 10_1101-2021_01_07_425794 224 8 RNA- RNA- NNP 10_1101-2021_01_07_425794 224 9 seq seq NN 10_1101-2021_01_07_425794 224 10 genes gene NNS 10_1101-2021_01_07_425794 224 11 included include VBN 10_1101-2021_01_07_425794 224 12 in in IN 10_1101-2021_01_07_425794 224 13 the the DT 10_1101-2021_01_07_425794 224 14 filtered filter VBN 10_1101-2021_01_07_425794 224 15 RNA RNA NNP 10_1101-2021_01_07_425794 224 16 - - HYPH 10_1101-2021_01_07_425794 224 17 seq seq NN 10_1101-2021_01_07_425794 224 18 data datum NNS 10_1101-2021_01_07_425794 224 19 . . . 10_1101-2021_01_07_425794 225 1 14,405 14,405 CD 10_1101-2021_01_07_425794 225 2 , , , 10_1101-2021_01_07_425794 225 3 14,561 14,561 CD 10_1101-2021_01_07_425794 225 4 and and CC 10_1101-2021_01_07_425794 225 5 14,508 14,508 CD 10_1101-2021_01_07_425794 225 6 microarray microarray JJ 10_1101-2021_01_07_425794 225 7 genes gene NNS 10_1101-2021_01_07_425794 225 8 were be VBD 10_1101-2021_01_07_425794 225 9 found find VBN 10_1101-2021_01_07_425794 225 10 to to TO 10_1101-2021_01_07_425794 225 11 be be VB 10_1101-2021_01_07_425794 225 12 matched match VBN 10_1101-2021_01_07_425794 225 13 with with IN 10_1101-2021_01_07_425794 225 14 RNA RNA NNP 10_1101-2021_01_07_425794 225 15 - - HYPH 10_1101-2021_01_07_425794 225 16 seq seq NN 10_1101-2021_01_07_425794 225 17 genes gene NNS 10_1101-2021_01_07_425794 225 18 from from IN 10_1101-2021_01_07_425794 225 19 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 225 20 , , , 10_1101-2021_01_07_425794 225 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 225 22 - - HYPH 10_1101-2021_01_07_425794 225 23 NCBI NCBI NNP 10_1101-2021_01_07_425794 225 24 and and CC 10_1101-2021_01_07_425794 225 25 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 225 26 - - HYPH 10_1101-2021_01_07_425794 225 27 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 225 28 annotations annotation NNS 10_1101-2021_01_07_425794 225 29 , , , 10_1101-2021_01_07_425794 225 30 respectively respectively RB 10_1101-2021_01_07_425794 225 31 . . . 10_1101-2021_01_07_425794 226 1 13,424 13,424 CD 10_1101-2021_01_07_425794 226 2 microarray microarray JJ 10_1101-2021_01_07_425794 226 3 genes gene NNS 10_1101-2021_01_07_425794 226 4 were be VBD 10_1101-2021_01_07_425794 226 5 found find VBN 10_1101-2021_01_07_425794 226 6 to to TO 10_1101-2021_01_07_425794 226 7 be be VB 10_1101-2021_01_07_425794 226 8 present present JJ 10_1101-2021_01_07_425794 226 9 in in IN 10_1101-2021_01_07_425794 226 10 all all DT 10_1101-2021_01_07_425794 226 11 three three CD 10_1101-2021_01_07_425794 226 12 annotations annotation NNS 10_1101-2021_01_07_425794 226 13 . . . 10_1101-2021_01_07_425794 227 1 For for IN 10_1101-2021_01_07_425794 227 2 those those DT 10_1101-2021_01_07_425794 227 3 microarray microarray JJ 10_1101-2021_01_07_425794 227 4 genes gene NNS 10_1101-2021_01_07_425794 227 5 that that WDT 10_1101-2021_01_07_425794 227 6 contain contain VBP 10_1101-2021_01_07_425794 227 7 more more JJR 10_1101-2021_01_07_425794 227 8 than than IN 10_1101-2021_01_07_425794 227 9 one one CD 10_1101-2021_01_07_425794 227 10 probe probe NN 10_1101-2021_01_07_425794 227 11 , , , 10_1101-2021_01_07_425794 227 12 a a DT 10_1101-2021_01_07_425794 227 13 representative representative JJ 10_1101-2021_01_07_425794 227 14 probe probe NN 10_1101-2021_01_07_425794 227 15 was be VBD 10_1101-2021_01_07_425794 227 16 selected select VBN 10_1101-2021_01_07_425794 227 17 for for IN 10_1101-2021_01_07_425794 227 18 each each DT 10_1101-2021_01_07_425794 227 19 of of IN 10_1101-2021_01_07_425794 227 20 them -PRON- PRP 10_1101-2021_01_07_425794 227 21 . . . 10_1101-2021_01_07_425794 228 1 The the DT 10_1101-2021_01_07_425794 228 2 representative representative JJ 10_1101-2021_01_07_425794 228 3 probe probe NN 10_1101-2021_01_07_425794 228 4 selected select VBN 10_1101-2021_01_07_425794 228 5 for for IN 10_1101-2021_01_07_425794 228 6 a a DT 10_1101-2021_01_07_425794 228 7 gene gene NN 10_1101-2021_01_07_425794 228 8 had have VBD 10_1101-2021_01_07_425794 228 9 the the DT 10_1101-2021_01_07_425794 228 10 highest high JJS 10_1101-2021_01_07_425794 228 11 mean mean JJ 10_1101-2021_01_07_425794 228 12 expression expression NN 10_1101-2021_01_07_425794 228 13 value value NN 10_1101-2021_01_07_425794 228 14 across across IN 10_1101-2021_01_07_425794 228 15 the the DT 10_1101-2021_01_07_425794 228 16 four four CD 10_1101-2021_01_07_425794 228 17 samples sample NNS 10_1101-2021_01_07_425794 228 18 among among IN 10_1101-2021_01_07_425794 228 19 all all PDT 10_1101-2021_01_07_425794 228 20 the the DT 10_1101-2021_01_07_425794 228 21 probes probe NNS 10_1101-2021_01_07_425794 228 22 the the DT 10_1101-2021_01_07_425794 228 23 gene gene NN 10_1101-2021_01_07_425794 228 24 has have VBZ 10_1101-2021_01_07_425794 228 25 . . . 10_1101-2021_01_07_425794 229 1 A a DT 10_1101-2021_01_07_425794 229 2 Pearson Pearson NNP 10_1101-2021_01_07_425794 229 3 correlation correlation NN 10_1101-2021_01_07_425794 229 4 analysis analysis NN 10_1101-2021_01_07_425794 229 5 was be VBD 10_1101-2021_01_07_425794 229 6 then then RB 10_1101-2021_01_07_425794 229 7 performed perform VBN 10_1101-2021_01_07_425794 229 8 between between IN 10_1101-2021_01_07_425794 229 9 microarray microarray JJ 10_1101-2021_01_07_425794 229 10 data datum NNS 10_1101-2021_01_07_425794 229 11 and and CC 10_1101-2021_01_07_425794 229 12 RNA RNA NNP 10_1101-2021_01_07_425794 229 13 - - HYPH 10_1101-2021_01_07_425794 229 14 seq seq NN 10_1101-2021_01_07_425794 229 15 data datum NNS 10_1101-2021_01_07_425794 229 16 for for IN 10_1101-2021_01_07_425794 229 17 each each DT 10_1101-2021_01_07_425794 229 18 of of IN 10_1101-2021_01_07_425794 229 19 the the DT 10_1101-2021_01_07_425794 229 20 three three CD 10_1101-2021_01_07_425794 229 21 annotations annotation NNS 10_1101-2021_01_07_425794 229 22 . . . 10_1101-2021_01_07_425794 230 1 Both both DT 10_1101-2021_01_07_425794 230 2 RNA RNA NNP 10_1101-2021_01_07_425794 230 3 - - HYPH 10_1101-2021_01_07_425794 230 4 seq seq NN 10_1101-2021_01_07_425794 230 5 and and CC 10_1101-2021_01_07_425794 230 6 microarray microarray NNP 10_1101-2021_01_07_425794 230 7 data datum NNS 10_1101-2021_01_07_425794 230 8 include include VBP 10_1101-2021_01_07_425794 230 9 log2 log2 NN 10_1101-2021_01_07_425794 230 10 expression expression NN 10_1101-2021_01_07_425794 230 11 values value NNS 10_1101-2021_01_07_425794 230 12 of of IN 10_1101-2021_01_07_425794 230 13 genes gene NNS 10_1101-2021_01_07_425794 230 14 . . . 10_1101-2021_01_07_425794 231 1 Figure figure NN 10_1101-2021_01_07_425794 231 2 7 7 CD 10_1101-2021_01_07_425794 231 3 shows show VBZ 10_1101-2021_01_07_425794 231 4 that that IN 10_1101-2021_01_07_425794 231 5 the the DT 10_1101-2021_01_07_425794 231 6 use use NN 10_1101-2021_01_07_425794 231 7 of of IN 10_1101-2021_01_07_425794 231 8 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 231 9 - - HYPH 10_1101-2021_01_07_425794 231 10 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 231 11 annotation annotation NN 10_1101-2021_01_07_425794 231 12 consistently consistently RB 10_1101-2021_01_07_425794 231 13 yielded yield VBD 10_1101-2021_01_07_425794 231 14 the the DT 10_1101-2021_01_07_425794 231 15 highest high JJS 10_1101-2021_01_07_425794 231 16 correlation correlation NN 10_1101-2021_01_07_425794 231 17 between between IN 10_1101-2021_01_07_425794 231 18 RNA RNA NNP 10_1101-2021_01_07_425794 231 19 - - HYPH 10_1101-2021_01_07_425794 231 20 seq seq NN 10_1101-2021_01_07_425794 231 21 and and CC 10_1101-2021_01_07_425794 231 22 microarray microarray JJ 10_1101-2021_01_07_425794 231 23 data datum NNS 10_1101-2021_01_07_425794 231 24 in in IN 10_1101-2021_01_07_425794 231 25 all all PDT 10_1101-2021_01_07_425794 231 26 the the DT 10_1101-2021_01_07_425794 231 27 comparisons comparison NNS 10_1101-2021_01_07_425794 231 28 , , , 10_1101-2021_01_07_425794 231 29 no no RB 10_1101-2021_01_07_425794 231 30 matter matter RB 10_1101-2021_01_07_425794 231 31 which which WDT 10_1101-2021_01_07_425794 231 32 RNA RNA NNP 10_1101-2021_01_07_425794 231 33 - - HYPH 10_1101-2021_01_07_425794 231 34 seq seq NNP 10_1101-2021_01_07_425794 231 35 normalization normalization NN 10_1101-2021_01_07_425794 231 36 method method NN 10_1101-2021_01_07_425794 231 37 was be VBD 10_1101-2021_01_07_425794 231 38 used use VBN 10_1101-2021_01_07_425794 231 39 and and CC 10_1101-2021_01_07_425794 231 40 if if IN 10_1101-2021_01_07_425794 231 41 all all DT 10_1101-2021_01_07_425794 231 42 or or CC 10_1101-2021_01_07_425794 231 43 common common JJ 10_1101-2021_01_07_425794 231 44 matched match VBN 10_1101-2021_01_07_425794 231 45 genes gene NNS 10_1101-2021_01_07_425794 231 46 were be VBD 10_1101-2021_01_07_425794 231 47 included include VBN 10_1101-2021_01_07_425794 231 48 in in IN 10_1101-2021_01_07_425794 231 49 the the DT 10_1101-2021_01_07_425794 231 50 evaluation evaluation NN 10_1101-2021_01_07_425794 231 51 . . . 10_1101-2021_01_07_425794 232 1 On on IN 10_1101-2021_01_07_425794 232 2 the the DT 10_1101-2021_01_07_425794 232 3 other other JJ 10_1101-2021_01_07_425794 232 4 hand hand NN 10_1101-2021_01_07_425794 232 5 , , , 10_1101-2021_01_07_425794 232 6 the the DT 10_1101-2021_01_07_425794 232 7 use use NN 10_1101-2021_01_07_425794 232 8 of of IN 10_1101-2021_01_07_425794 232 9 the the DT 10_1101-2021_01_07_425794 232 10 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 232 11 annotation annotation NN 10_1101-2021_01_07_425794 232 12 resulted result VBD 10_1101-2021_01_07_425794 232 13 in in IN 10_1101-2021_01_07_425794 232 14 the the DT 10_1101-2021_01_07_425794 232 15 worst bad JJS 10_1101-2021_01_07_425794 232 16 correlation correlation NN 10_1101-2021_01_07_425794 232 17 between between IN 10_1101-2021_01_07_425794 232 18 RNA RNA NNP 10_1101-2021_01_07_425794 232 19 - - HYPH 10_1101-2021_01_07_425794 232 20 seq seq NN 10_1101-2021_01_07_425794 232 21 data datum NNS 10_1101-2021_01_07_425794 232 22 and and CC 10_1101-2021_01_07_425794 232 23 microarray microarray JJ 10_1101-2021_01_07_425794 232 24 data datum NNS 10_1101-2021_01_07_425794 232 25 in in IN 10_1101-2021_01_07_425794 232 26 all all PDT 10_1101-2021_01_07_425794 232 27 the the DT 10_1101-2021_01_07_425794 232 28 comparisons comparison NNS 10_1101-2021_01_07_425794 232 29 . . . 10_1101-2021_01_07_425794 233 1 17 17 CD 10_1101-2021_01_07_425794 233 2 .CC .CC : 10_1101-2021_01_07_425794 233 3 - - HYPH 10_1101-2021_01_07_425794 233 4 BY by IN 10_1101-2021_01_07_425794 233 5 - - HYPH 10_1101-2021_01_07_425794 233 6 NC NC NNP 10_1101-2021_01_07_425794 233 7 - - HYPH 10_1101-2021_01_07_425794 233 8 ND ND NNP 10_1101-2021_01_07_425794 233 9 4.0 4.0 CD 10_1101-2021_01_07_425794 233 10 International international JJ 10_1101-2021_01_07_425794 233 11 licensemade licensemade NN 10_1101-2021_01_07_425794 233 12 available available JJ 10_1101-2021_01_07_425794 233 13 under under IN 10_1101-2021_01_07_425794 233 14 a a DT 10_1101-2021_01_07_425794 233 15 ( ( -LRB- 10_1101-2021_01_07_425794 233 16 which which WDT 10_1101-2021_01_07_425794 233 17 was be VBD 10_1101-2021_01_07_425794 233 18 not not RB 10_1101-2021_01_07_425794 233 19 certified certify VBN 10_1101-2021_01_07_425794 233 20 by by IN 10_1101-2021_01_07_425794 233 21 peer peer NN 10_1101-2021_01_07_425794 233 22 review review NN 10_1101-2021_01_07_425794 233 23 ) ) -RRB- 10_1101-2021_01_07_425794 233 24 is be VBZ 10_1101-2021_01_07_425794 233 25 the the DT 10_1101-2021_01_07_425794 233 26 author author NN 10_1101-2021_01_07_425794 233 27 / / SYM 10_1101-2021_01_07_425794 233 28 funder funder NN 10_1101-2021_01_07_425794 233 29 , , , 10_1101-2021_01_07_425794 233 30 who who WP 10_1101-2021_01_07_425794 233 31 has have VBZ 10_1101-2021_01_07_425794 233 32 granted grant VBN 10_1101-2021_01_07_425794 233 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 233 34 a a DT 10_1101-2021_01_07_425794 233 35 license license NN 10_1101-2021_01_07_425794 233 36 to to TO 10_1101-2021_01_07_425794 233 37 display display VB 10_1101-2021_01_07_425794 233 38 the the DT 10_1101-2021_01_07_425794 233 39 preprint preprint NN 10_1101-2021_01_07_425794 233 40 in in IN 10_1101-2021_01_07_425794 233 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 233 42 . . . 10_1101-2021_01_07_425794 234 1 It -PRON- PRP 10_1101-2021_01_07_425794 234 2 is be VBZ 10_1101-2021_01_07_425794 234 3 The the DT 10_1101-2021_01_07_425794 234 4 copyright copyright NN 10_1101-2021_01_07_425794 234 5 holder holder NN 10_1101-2021_01_07_425794 234 6 for for IN 10_1101-2021_01_07_425794 234 7 this this DT 10_1101-2021_01_07_425794 234 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 234 9 version version NN 10_1101-2021_01_07_425794 234 10 posted post VBD 10_1101-2021_01_07_425794 234 11 January January NNP 10_1101-2021_01_07_425794 234 12 8 8 CD 10_1101-2021_01_07_425794 234 13 , , , 10_1101-2021_01_07_425794 234 14 2021 2021 CD 10_1101-2021_01_07_425794 234 15 . . . 10_1101-2021_01_07_425794 234 16 ; ; : 10_1101-2021_01_07_425794 234 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 234 18 : : : 10_1101-2021_01_07_425794 234 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 234 20 preprint preprint JJ 10_1101-2021_01_07_425794 234 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 234 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 234 23 C C NNP 10_1101-2021_01_07_425794 234 24 or or CC 10_1101-2021_01_07_425794 234 25 re re VB 10_1101-2021_01_07_425794 234 26 la la NNP 10_1101-2021_01_07_425794 234 27 tio tio NNP 10_1101-2021_01_07_425794 234 28 n n NNP 10_1101-2021_01_07_425794 234 29 co co NNP 10_1101-2021_01_07_425794 234 30 ef ef NNP 10_1101-2021_01_07_425794 234 31 fic fic NNP 10_1101-2021_01_07_425794 234 32 ie ie NNP 10_1101-2021_01_07_425794 234 33 nt nt RB 10_1101-2021_01_07_425794 234 34 All all DT 10_1101-2021_01_07_425794 234 35 genes gene NNS 10_1101-2021_01_07_425794 234 36 after after IN 10_1101-2021_01_07_425794 234 37 filtering filter VBG 10_1101-2021_01_07_425794 234 38 Common common JJ 10_1101-2021_01_07_425794 234 39 genes gene NNS 10_1101-2021_01_07_425794 234 40 after after IN 10_1101-2021_01_07_425794 234 41 filtering filter VBG 10_1101-2021_01_07_425794 234 42 C C NNP 10_1101-2021_01_07_425794 234 43 or or CC 10_1101-2021_01_07_425794 234 44 re re VB 10_1101-2021_01_07_425794 234 45 la la NNP 10_1101-2021_01_07_425794 234 46 tio tio NNP 10_1101-2021_01_07_425794 234 47 n n NNP 10_1101-2021_01_07_425794 234 48 co co NNP 10_1101-2021_01_07_425794 234 49 ef ef NNP 10_1101-2021_01_07_425794 234 50 fic fic NNP 10_1101-2021_01_07_425794 234 51 ie ie NNP 10_1101-2021_01_07_425794 234 52 nt nt RB 10_1101-2021_01_07_425794 234 53 0.60 0.60 CD 10_1101-2021_01_07_425794 234 54 0.65 0.65 CD 10_1101-2021_01_07_425794 234 55 0.70 0.70 CD 10_1101-2021_01_07_425794 234 56 0.75 0.75 CD 10_1101-2021_01_07_425794 234 57 A a NN 10_1101-2021_01_07_425794 234 58 B b NN 10_1101-2021_01_07_425794 234 59 C c NN 10_1101-2021_01_07_425794 234 60 D d NN 10_1101-2021_01_07_425794 234 61 A a NN 10_1101-2021_01_07_425794 234 62 B b NN 10_1101-2021_01_07_425794 234 63 C c NN 10_1101-2021_01_07_425794 234 64 D D NNP 10_1101-2021_01_07_425794 234 65 Li Li NNP 10_1101-2021_01_07_425794 234 66 br br NN 10_1101-2021_01_07_425794 234 67 ar ar NNP 10_1101-2021_01_07_425794 234 68 y y NNP 10_1101-2021_01_07_425794 234 69 si si NN 10_1101-2021_01_07_425794 234 70 ze ze UH 10_1101-2021_01_07_425794 234 71 no no UH 10_1101-2021_01_07_425794 234 72 rm rm NNP 10_1101-2021_01_07_425794 234 73 al al NNP 10_1101-2021_01_07_425794 234 74 iz iz NNP 10_1101-2021_01_07_425794 234 75 at at IN 10_1101-2021_01_07_425794 234 76 io io NNP 10_1101-2021_01_07_425794 234 77 n n NNP 10_1101-2021_01_07_425794 234 78 Q Q NNP 10_1101-2021_01_07_425794 234 79 ua ua PRP 10_1101-2021_01_07_425794 234 80 nt nt RB 10_1101-2021_01_07_425794 234 81 ile ile VB 10_1101-2021_01_07_425794 234 82 no no UH 10_1101-2021_01_07_425794 234 83 rm rm NNP 10_1101-2021_01_07_425794 234 84 al al NNP 10_1101-2021_01_07_425794 234 85 iz iz NNP 10_1101-2021_01_07_425794 234 86 at at IN 10_1101-2021_01_07_425794 234 87 io io NNP 10_1101-2021_01_07_425794 234 88 n n CC 10_1101-2021_01_07_425794 234 89 TM TM NNP 10_1101-2021_01_07_425794 234 90 M M NNP 10_1101-2021_01_07_425794 234 91 no no UH 10_1101-2021_01_07_425794 234 92 rm rm NNP 10_1101-2021_01_07_425794 234 93 al al NNP 10_1101-2021_01_07_425794 234 94 iz iz NNP 10_1101-2021_01_07_425794 234 95 at at IN 10_1101-2021_01_07_425794 234 96 io io NNP 10_1101-2021_01_07_425794 234 97 n n CC 10_1101-2021_01_07_425794 234 98 C C NNP 10_1101-2021_01_07_425794 234 99 or or CC 10_1101-2021_01_07_425794 234 100 re re VB 10_1101-2021_01_07_425794 234 101 la la NNP 10_1101-2021_01_07_425794 234 102 tio tio NNP 10_1101-2021_01_07_425794 234 103 n n NNP 10_1101-2021_01_07_425794 234 104 co co NNP 10_1101-2021_01_07_425794 234 105 ef ef NNP 10_1101-2021_01_07_425794 234 106 fic fic NNP 10_1101-2021_01_07_425794 234 107 ie ie NNP 10_1101-2021_01_07_425794 234 108 nt nt RB 10_1101-2021_01_07_425794 234 109 0.80 0.80 CD 10_1101-2021_01_07_425794 234 110 0.60 0.60 CD 10_1101-2021_01_07_425794 234 111 0.65 0.65 CD 10_1101-2021_01_07_425794 234 112 0.70 0.70 CD 10_1101-2021_01_07_425794 234 113 0.75 0.75 CD 10_1101-2021_01_07_425794 234 114 0.80 0.80 CD 10_1101-2021_01_07_425794 234 115 0.60 0.60 CD 10_1101-2021_01_07_425794 234 116 0.65 0.65 CD 10_1101-2021_01_07_425794 234 117 0.70 0.70 CD 10_1101-2021_01_07_425794 234 118 0.75 0.75 CD 10_1101-2021_01_07_425794 234 119 0.80 0.80 CD 10_1101-2021_01_07_425794 234 120 0.60 0.60 CD 10_1101-2021_01_07_425794 234 121 0.65 0.65 CD 10_1101-2021_01_07_425794 234 122 0.70 0.70 CD 10_1101-2021_01_07_425794 234 123 0.75 0.75 CD 10_1101-2021_01_07_425794 234 124 0.80 0.80 CD 10_1101-2021_01_07_425794 234 125 0.60 0.60 CD 10_1101-2021_01_07_425794 234 126 0.65 0.65 CD 10_1101-2021_01_07_425794 234 127 0.70 0.70 CD 10_1101-2021_01_07_425794 234 128 0.75 0.75 CD 10_1101-2021_01_07_425794 234 129 0.80 0.80 CD 10_1101-2021_01_07_425794 234 130 0.60 0.60 CD 10_1101-2021_01_07_425794 234 131 0.65 0.65 CD 10_1101-2021_01_07_425794 234 132 0.70 0.70 CD 10_1101-2021_01_07_425794 234 133 0.75 0.75 CD 10_1101-2021_01_07_425794 234 134 0.80 0.80 CD 10_1101-2021_01_07_425794 234 135 Key Key NNP 10_1101-2021_01_07_425794 234 136 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 234 137 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 234 138 - - HYPH 10_1101-2021_01_07_425794 234 139 NCBI NCBI NNP 10_1101-2021_01_07_425794 234 140 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 234 141 - - HYPH 10_1101-2021_01_07_425794 234 142 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 234 143 Figure Figure NNP 10_1101-2021_01_07_425794 234 144 7 7 CD 10_1101-2021_01_07_425794 234 145 : : : 10_1101-2021_01_07_425794 234 146 Validation validation NN 10_1101-2021_01_07_425794 234 147 of of IN 10_1101-2021_01_07_425794 234 148 RNA RNA NNP 10_1101-2021_01_07_425794 234 149 - - HYPH 10_1101-2021_01_07_425794 234 150 seq seq NN 10_1101-2021_01_07_425794 234 151 quantification quantification NN 10_1101-2021_01_07_425794 234 152 results result NNS 10_1101-2021_01_07_425794 234 153 against against IN 10_1101-2021_01_07_425794 234 154 microarray microarray JJ 10_1101-2021_01_07_425794 234 155 data datum NNS 10_1101-2021_01_07_425794 234 156 . . . 10_1101-2021_01_07_425794 235 1 Shown show VBN 10_1101-2021_01_07_425794 235 2 are be VBP 10_1101-2021_01_07_425794 235 3 Pearson Pearson NNP 10_1101-2021_01_07_425794 235 4 correlation correlation NN 10_1101-2021_01_07_425794 235 5 coefficients coefficient NNS 10_1101-2021_01_07_425794 235 6 computed compute VBD 10_1101-2021_01_07_425794 235 7 from from IN 10_1101-2021_01_07_425794 235 8 comparing compare VBG 10_1101-2021_01_07_425794 235 9 RNA RNA NNP 10_1101-2021_01_07_425794 235 10 - - HYPH 10_1101-2021_01_07_425794 235 11 seq seq NN 10_1101-2021_01_07_425794 235 12 data datum NNS 10_1101-2021_01_07_425794 235 13 against against IN 10_1101-2021_01_07_425794 235 14 Illumina Illumina NNP 10_1101-2021_01_07_425794 235 15 BeadChip BeadChip NNP 10_1101-2021_01_07_425794 235 16 microarray microarray JJ 10_1101-2021_01_07_425794 235 17 data datum NNS 10_1101-2021_01_07_425794 235 18 , , , 10_1101-2021_01_07_425794 235 19 using use VBG 10_1101-2021_01_07_425794 235 20 the the DT 10_1101-2021_01_07_425794 235 21 microarray microarray JJ 10_1101-2021_01_07_425794 235 22 genes gene NNS 10_1101-2021_01_07_425794 235 23 matched match VBN 10_1101-2021_01_07_425794 235 24 with with IN 10_1101-2021_01_07_425794 235 25 each each DT 10_1101-2021_01_07_425794 235 26 individual individual JJ 10_1101-2021_01_07_425794 235 27 annotation annotation NN 10_1101-2021_01_07_425794 235 28 ( ( -LRB- 10_1101-2021_01_07_425794 235 29 left leave VBN 10_1101-2021_01_07_425794 235 30 column column NN 10_1101-2021_01_07_425794 235 31 ) ) -RRB- 10_1101-2021_01_07_425794 235 32 or or CC 10_1101-2021_01_07_425794 235 33 matched match VBN 10_1101-2021_01_07_425794 235 34 with with IN 10_1101-2021_01_07_425794 235 35 all all DT 10_1101-2021_01_07_425794 235 36 three three CD 10_1101-2021_01_07_425794 235 37 annotations annotation NNS 10_1101-2021_01_07_425794 235 38 ( ( -LRB- 10_1101-2021_01_07_425794 235 39 right right JJ 10_1101-2021_01_07_425794 235 40 column column NN 10_1101-2021_01_07_425794 235 41 ) ) -RRB- 10_1101-2021_01_07_425794 235 42 . . . 10_1101-2021_01_07_425794 236 1 Rows row NNS 10_1101-2021_01_07_425794 236 2 in in IN 10_1101-2021_01_07_425794 236 3 the the DT 10_1101-2021_01_07_425794 236 4 plots plot NNS 10_1101-2021_01_07_425794 236 5 represent represent VBP 10_1101-2021_01_07_425794 236 6 the the DT 10_1101-2021_01_07_425794 236 7 different different JJ 10_1101-2021_01_07_425794 236 8 RNA RNA NNP 10_1101-2021_01_07_425794 236 9 - - HYPH 10_1101-2021_01_07_425794 236 10 seq seq NN 10_1101-2021_01_07_425794 236 11 normalization normalization NN 10_1101-2021_01_07_425794 236 12 methods method NNS 10_1101-2021_01_07_425794 236 13 used use VBN 10_1101-2021_01_07_425794 236 14 . . . 10_1101-2021_01_07_425794 237 1 Lowly Lowly NNP 10_1101-2021_01_07_425794 237 2 expressed express VBD 10_1101-2021_01_07_425794 237 3 genes gene NNS 10_1101-2021_01_07_425794 237 4 in in IN 10_1101-2021_01_07_425794 237 5 the the DT 10_1101-2021_01_07_425794 237 6 RNA RNA NNP 10_1101-2021_01_07_425794 237 7 - - HYPH 10_1101-2021_01_07_425794 237 8 seq seq NN 10_1101-2021_01_07_425794 237 9 data datum NNS 10_1101-2021_01_07_425794 237 10 were be VBD 10_1101-2021_01_07_425794 237 11 filtered filter VBN 10_1101-2021_01_07_425794 237 12 out out RP 10_1101-2021_01_07_425794 237 13 before before IN 10_1101-2021_01_07_425794 237 14 the the DT 10_1101-2021_01_07_425794 237 15 correlation correlation NN 10_1101-2021_01_07_425794 237 16 analysis analysis NN 10_1101-2021_01_07_425794 237 17 was be VBD 10_1101-2021_01_07_425794 237 18 performed perform VBN 10_1101-2021_01_07_425794 237 19 . . . 10_1101-2021_01_07_425794 238 1 For for IN 10_1101-2021_01_07_425794 238 2 those those DT 10_1101-2021_01_07_425794 238 3 microarray microarray JJ 10_1101-2021_01_07_425794 238 4 genes gene NNS 10_1101-2021_01_07_425794 238 5 that that WDT 10_1101-2021_01_07_425794 238 6 include include VBP 10_1101-2021_01_07_425794 238 7 more more JJR 10_1101-2021_01_07_425794 238 8 than than IN 10_1101-2021_01_07_425794 238 9 one one CD 10_1101-2021_01_07_425794 238 10 probe probe NN 10_1101-2021_01_07_425794 238 11 , , , 10_1101-2021_01_07_425794 238 12 a a DT 10_1101-2021_01_07_425794 238 13 representative representative JJ 10_1101-2021_01_07_425794 238 14 probe probe NN 10_1101-2021_01_07_425794 238 15 was be VBD 10_1101-2021_01_07_425794 238 16 selected select VBN 10_1101-2021_01_07_425794 238 17 and and CC 10_1101-2021_01_07_425794 238 18 used use VBN 10_1101-2021_01_07_425794 238 19 for for IN 10_1101-2021_01_07_425794 238 20 this this DT 10_1101-2021_01_07_425794 238 21 analysis analysis NN 10_1101-2021_01_07_425794 238 22 . . . 10_1101-2021_01_07_425794 239 1 18 18 CD 10_1101-2021_01_07_425794 239 2 .CC .CC : 10_1101-2021_01_07_425794 239 3 - - HYPH 10_1101-2021_01_07_425794 239 4 BY by IN 10_1101-2021_01_07_425794 239 5 - - HYPH 10_1101-2021_01_07_425794 239 6 NC NC NNP 10_1101-2021_01_07_425794 239 7 - - HYPH 10_1101-2021_01_07_425794 239 8 ND ND NNP 10_1101-2021_01_07_425794 239 9 4.0 4.0 CD 10_1101-2021_01_07_425794 239 10 International international JJ 10_1101-2021_01_07_425794 239 11 licensemade licensemade NN 10_1101-2021_01_07_425794 239 12 available available JJ 10_1101-2021_01_07_425794 239 13 under under IN 10_1101-2021_01_07_425794 239 14 a a DT 10_1101-2021_01_07_425794 239 15 ( ( -LRB- 10_1101-2021_01_07_425794 239 16 which which WDT 10_1101-2021_01_07_425794 239 17 was be VBD 10_1101-2021_01_07_425794 239 18 not not RB 10_1101-2021_01_07_425794 239 19 certified certify VBN 10_1101-2021_01_07_425794 239 20 by by IN 10_1101-2021_01_07_425794 239 21 peer peer NN 10_1101-2021_01_07_425794 239 22 review review NN 10_1101-2021_01_07_425794 239 23 ) ) -RRB- 10_1101-2021_01_07_425794 239 24 is be VBZ 10_1101-2021_01_07_425794 239 25 the the DT 10_1101-2021_01_07_425794 239 26 author author NN 10_1101-2021_01_07_425794 239 27 / / SYM 10_1101-2021_01_07_425794 239 28 funder funder NN 10_1101-2021_01_07_425794 239 29 , , , 10_1101-2021_01_07_425794 239 30 who who WP 10_1101-2021_01_07_425794 239 31 has have VBZ 10_1101-2021_01_07_425794 239 32 granted grant VBN 10_1101-2021_01_07_425794 239 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 239 34 a a DT 10_1101-2021_01_07_425794 239 35 license license NN 10_1101-2021_01_07_425794 239 36 to to TO 10_1101-2021_01_07_425794 239 37 display display VB 10_1101-2021_01_07_425794 239 38 the the DT 10_1101-2021_01_07_425794 239 39 preprint preprint NN 10_1101-2021_01_07_425794 239 40 in in IN 10_1101-2021_01_07_425794 239 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 239 42 . . . 10_1101-2021_01_07_425794 240 1 It -PRON- PRP 10_1101-2021_01_07_425794 240 2 is be VBZ 10_1101-2021_01_07_425794 240 3 The the DT 10_1101-2021_01_07_425794 240 4 copyright copyright NN 10_1101-2021_01_07_425794 240 5 holder holder NN 10_1101-2021_01_07_425794 240 6 for for IN 10_1101-2021_01_07_425794 240 7 this this DT 10_1101-2021_01_07_425794 240 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 240 9 version version NN 10_1101-2021_01_07_425794 240 10 posted post VBD 10_1101-2021_01_07_425794 240 11 January January NNP 10_1101-2021_01_07_425794 240 12 8 8 CD 10_1101-2021_01_07_425794 240 13 , , , 10_1101-2021_01_07_425794 240 14 2021 2021 CD 10_1101-2021_01_07_425794 240 15 . . . 10_1101-2021_01_07_425794 240 16 ; ; : 10_1101-2021_01_07_425794 240 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 240 18 : : : 10_1101-2021_01_07_425794 240 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 240 20 preprint preprint JJ 10_1101-2021_01_07_425794 240 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 240 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 240 23 4 4 CD 10_1101-2021_01_07_425794 240 24 DISCUSSION DISCUSSION NNP 10_1101-2021_01_07_425794 240 25 The the DT 10_1101-2021_01_07_425794 240 26 RNA RNA NNP 10_1101-2021_01_07_425794 240 27 - - HYPH 10_1101-2021_01_07_425794 240 28 seq seq NN 10_1101-2021_01_07_425794 240 29 technique technique NN 10_1101-2021_01_07_425794 240 30 is be VBZ 10_1101-2021_01_07_425794 240 31 currently currently RB 10_1101-2021_01_07_425794 240 32 routinely routinely RB 10_1101-2021_01_07_425794 240 33 used use VBN 10_1101-2021_01_07_425794 240 34 for for IN 10_1101-2021_01_07_425794 240 35 genome genome NN 10_1101-2021_01_07_425794 240 36 - - HYPH 10_1101-2021_01_07_425794 240 37 wide wide JJ 10_1101-2021_01_07_425794 240 38 profiling profiling NN 10_1101-2021_01_07_425794 240 39 of of IN 10_1101-2021_01_07_425794 240 40 gene gene NN 10_1101-2021_01_07_425794 240 41 expression expression NN 10_1101-2021_01_07_425794 240 42 in in IN 10_1101-2021_01_07_425794 240 43 the the DT 10_1101-2021_01_07_425794 240 44 biomedical biomedical JJ 10_1101-2021_01_07_425794 240 45 research research NN 10_1101-2021_01_07_425794 240 46 field field NN 10_1101-2021_01_07_425794 240 47 . . . 10_1101-2021_01_07_425794 241 1 The the DT 10_1101-2021_01_07_425794 241 2 analysis analysis NN 10_1101-2021_01_07_425794 241 3 of of IN 10_1101-2021_01_07_425794 241 4 RNA RNA NNP 10_1101-2021_01_07_425794 241 5 - - HYPH 10_1101-2021_01_07_425794 241 6 seq seq NN 10_1101-2021_01_07_425794 241 7 data data NN 10_1101-2021_01_07_425794 241 8 relies rely VBZ 10_1101-2021_01_07_425794 241 9 on on IN 10_1101-2021_01_07_425794 241 10 the the DT 10_1101-2021_01_07_425794 241 11 accurate accurate JJ 10_1101-2021_01_07_425794 241 12 annotation annotation NN 10_1101-2021_01_07_425794 241 13 of of IN 10_1101-2021_01_07_425794 241 14 genes gene NNS 10_1101-2021_01_07_425794 241 15 so so IN 10_1101-2021_01_07_425794 241 16 that that IN 10_1101-2021_01_07_425794 241 17 expression expression NN 10_1101-2021_01_07_425794 241 18 levels level NNS 10_1101-2021_01_07_425794 241 19 of of IN 10_1101-2021_01_07_425794 241 20 genes gene NNS 10_1101-2021_01_07_425794 241 21 can can MD 10_1101-2021_01_07_425794 241 22 be be VB 10_1101-2021_01_07_425794 241 23 accurately accurately RB 10_1101-2021_01_07_425794 241 24 and and CC 10_1101-2021_01_07_425794 241 25 re- re- RB 10_1101-2021_01_07_425794 241 26 liably liably RB 10_1101-2021_01_07_425794 241 27 quantified quantify VBN 10_1101-2021_01_07_425794 241 28 . . . 10_1101-2021_01_07_425794 242 1 There there EX 10_1101-2021_01_07_425794 242 2 are be VBP 10_1101-2021_01_07_425794 242 3 several several JJ 10_1101-2021_01_07_425794 242 4 major major JJ 10_1101-2021_01_07_425794 242 5 gene gene NN 10_1101-2021_01_07_425794 242 6 annotation annotation NN 10_1101-2021_01_07_425794 242 7 sources source NNS 10_1101-2021_01_07_425794 242 8 that that WDT 10_1101-2021_01_07_425794 242 9 have have VBP 10_1101-2021_01_07_425794 242 10 been be VBN 10_1101-2021_01_07_425794 242 11 widely widely RB 10_1101-2021_01_07_425794 242 12 adopted adopt VBN 10_1101-2021_01_07_425794 242 13 in in IN 10_1101-2021_01_07_425794 242 14 the the DT 10_1101-2021_01_07_425794 242 15 field field NN 10_1101-2021_01_07_425794 242 16 such such JJ 10_1101-2021_01_07_425794 242 17 as as IN 10_1101-2021_01_07_425794 242 18 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 242 19 and and CC 10_1101-2021_01_07_425794 242 20 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 242 21 annotations annotation NNS 10_1101-2021_01_07_425794 242 22 . . . 10_1101-2021_01_07_425794 243 1 The the DT 10_1101-2021_01_07_425794 243 2 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 243 3 and and CC 10_1101-2021_01_07_425794 243 4 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 243 5 annotations annotation NNS 10_1101-2021_01_07_425794 243 6 have have VBP 10_1101-2021_01_07_425794 243 7 been be VBN 10_1101-2021_01_07_425794 243 8 well well RB 10_1101-2021_01_07_425794 243 9 maintained maintain VBN 10_1101-2021_01_07_425794 243 10 and and CC 10_1101-2021_01_07_425794 243 11 under under IN 10_1101-2021_01_07_425794 243 12 continuous continuous JJ 10_1101-2021_01_07_425794 243 13 development development NN 10_1101-2021_01_07_425794 243 14 . . . 10_1101-2021_01_07_425794 244 1 In in IN 10_1101-2021_01_07_425794 244 2 particular particular JJ 10_1101-2021_01_07_425794 244 3 , , , 10_1101-2021_01_07_425794 244 4 new new JJ 10_1101-2021_01_07_425794 244 5 gene gene NN 10_1101-2021_01_07_425794 244 6 information information NN 10_1101-2021_01_07_425794 244 7 collected collect VBN 10_1101-2021_01_07_425794 244 8 from from IN 10_1101-2021_01_07_425794 244 9 the the DT 10_1101-2021_01_07_425794 244 10 next next JJ 10_1101-2021_01_07_425794 244 11 - - HYPH 10_1101-2021_01_07_425794 244 12 generation generation NN 10_1101-2021_01_07_425794 244 13 sequencing sequencing NN 10_1101-2021_01_07_425794 244 14 technologies technology NNS 10_1101-2021_01_07_425794 244 15 , , , 10_1101-2021_01_07_425794 244 16 such such JJ 10_1101-2021_01_07_425794 244 17 as as IN 10_1101-2021_01_07_425794 244 18 RNA RNA NNP 10_1101-2021_01_07_425794 244 19 - - HYPH 10_1101-2021_01_07_425794 244 20 seq seq NNP 10_1101-2021_01_07_425794 244 21 , , , 10_1101-2021_01_07_425794 244 22 has have VBZ 10_1101-2021_01_07_425794 244 23 been be VBN 10_1101-2021_01_07_425794 244 24 incorporated incorporate VBN 10_1101-2021_01_07_425794 244 25 into into IN 10_1101-2021_01_07_425794 244 26 the the DT 10_1101-2021_01_07_425794 244 27 expansion expansion NN 10_1101-2021_01_07_425794 244 28 of of IN 10_1101-2021_01_07_425794 244 29 these these DT 10_1101-2021_01_07_425794 244 30 annotations annotation NNS 10_1101-2021_01_07_425794 244 31 in in IN 10_1101-2021_01_07_425794 244 32 recent recent JJ 10_1101-2021_01_07_425794 244 33 years year NNS 10_1101-2021_01_07_425794 244 34 . . . 10_1101-2021_01_07_425794 245 1 However however RB 10_1101-2021_01_07_425794 245 2 , , , 10_1101-2021_01_07_425794 245 3 differences difference NNS 10_1101-2021_01_07_425794 245 4 between between IN 10_1101-2021_01_07_425794 245 5 these these DT 10_1101-2021_01_07_425794 245 6 annotations annotation NNS 10_1101-2021_01_07_425794 245 7 have have VBP 10_1101-2021_01_07_425794 245 8 raised raise VBN 10_1101-2021_01_07_425794 245 9 concerns concern NNS 10_1101-2021_01_07_425794 245 10 over over IN 10_1101-2021_01_07_425794 245 11 the the DT 10_1101-2021_01_07_425794 245 12 quality quality NN 10_1101-2021_01_07_425794 245 13 and and CC 10_1101-2021_01_07_425794 245 14 reproducibility reproducibility NN 10_1101-2021_01_07_425794 245 15 of of IN 10_1101-2021_01_07_425794 245 16 RNA RNA NNP 10_1101-2021_01_07_425794 245 17 - - HYPH 10_1101-2021_01_07_425794 245 18 seq seq NN 10_1101-2021_01_07_425794 245 19 data data NN 10_1101-2021_01_07_425794 245 20 analyses analysis NNS 10_1101-2021_01_07_425794 245 21 . . . 10_1101-2021_01_07_425794 246 1 There there EX 10_1101-2021_01_07_425794 246 2 are be VBP 10_1101-2021_01_07_425794 246 3 particularly particularly RB 10_1101-2021_01_07_425794 246 4 concerns concern NNS 10_1101-2021_01_07_425794 246 5 regarding regard VBG 10_1101-2021_01_07_425794 246 6 the the DT 10_1101-2021_01_07_425794 246 7 accuracy accuracy NN 10_1101-2021_01_07_425794 246 8 of of IN 10_1101-2021_01_07_425794 246 9 new new JJ 10_1101-2021_01_07_425794 246 10 gene gene NN 10_1101-2021_01_07_425794 246 11 annotations annotation NNS 10_1101-2021_01_07_425794 246 12 generated generate VBN 10_1101-2021_01_07_425794 246 13 from from IN 10_1101-2021_01_07_425794 246 14 the the DT 10_1101-2021_01_07_425794 246 15 use use NN 10_1101-2021_01_07_425794 246 16 of of IN 10_1101-2021_01_07_425794 246 17 the the DT 10_1101-2021_01_07_425794 246 18 sequencing sequence VBG 10_1101-2021_01_07_425794 246 19 tech- tech- JJ 10_1101-2021_01_07_425794 246 20 nologies nologie NNS 10_1101-2021_01_07_425794 246 21 , , , 10_1101-2021_01_07_425794 246 22 due due JJ 10_1101-2021_01_07_425794 246 23 to to IN 10_1101-2021_01_07_425794 246 24 known know VBN 10_1101-2021_01_07_425794 246 25 errors error NNS 10_1101-2021_01_07_425794 246 26 in in IN 10_1101-2021_01_07_425794 246 27 the the DT 10_1101-2021_01_07_425794 246 28 generation generation NN 10_1101-2021_01_07_425794 246 29 and and CC 10_1101-2021_01_07_425794 246 30 analysis analysis NN 10_1101-2021_01_07_425794 246 31 of of IN 10_1101-2021_01_07_425794 246 32 the the DT 10_1101-2021_01_07_425794 246 33 sequencing sequencing NN 10_1101-2021_01_07_425794 246 34 data datum NNS 10_1101-2021_01_07_425794 246 35 . . . 10_1101-2021_01_07_425794 247 1 To to TO 10_1101-2021_01_07_425794 247 2 address address VB 10_1101-2021_01_07_425794 247 3 these these DT 10_1101-2021_01_07_425794 247 4 concerns concern NNS 10_1101-2021_01_07_425794 247 5 , , , 10_1101-2021_01_07_425794 247 6 in in IN 10_1101-2021_01_07_425794 247 7 this this DT 10_1101-2021_01_07_425794 247 8 study study NN 10_1101-2021_01_07_425794 247 9 we -PRON- PRP 10_1101-2021_01_07_425794 247 10 systematically systematically RB 10_1101-2021_01_07_425794 247 11 assessed assess VBD 10_1101-2021_01_07_425794 247 12 the the DT 10_1101-2021_01_07_425794 247 13 differences difference NNS 10_1101-2021_01_07_425794 247 14 in in IN 10_1101-2021_01_07_425794 247 15 RNA- RNA- NNP 10_1101-2021_01_07_425794 247 16 seq seq NN 10_1101-2021_01_07_425794 247 17 quantification quantification NN 10_1101-2021_01_07_425794 247 18 results result NNS 10_1101-2021_01_07_425794 247 19 attributed attribute VBN 10_1101-2021_01_07_425794 247 20 to to IN 10_1101-2021_01_07_425794 247 21 the the DT 10_1101-2021_01_07_425794 247 22 gene gene NN 10_1101-2021_01_07_425794 247 23 annotation annotation NN 10_1101-2021_01_07_425794 247 24 discrepancy discrepancy NN 10_1101-2021_01_07_425794 247 25 . . . 10_1101-2021_01_07_425794 248 1 Annotations annotation NNS 10_1101-2021_01_07_425794 248 2 being be VBG 10_1101-2021_01_07_425794 248 3 evaluated evaluate VBN 10_1101-2021_01_07_425794 248 4 in in IN 10_1101-2021_01_07_425794 248 5 this this DT 10_1101-2021_01_07_425794 248 6 study study NN 10_1101-2021_01_07_425794 248 7 included include VBD 10_1101-2021_01_07_425794 248 8 recent recent JJ 10_1101-2021_01_07_425794 248 9 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 248 10 and and CC 10_1101-2021_01_07_425794 248 11 NCBI NCBI NNP 10_1101-2021_01_07_425794 248 12 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 248 13 annotations annotation NNS 10_1101-2021_01_07_425794 248 14 and and CC 10_1101-2021_01_07_425794 248 15 also also RB 10_1101-2021_01_07_425794 248 16 an an DT 10_1101-2021_01_07_425794 248 17 older old JJR 10_1101-2021_01_07_425794 248 18 version version NN 10_1101-2021_01_07_425794 248 19 of of IN 10_1101-2021_01_07_425794 248 20 the the DT 10_1101-2021_01_07_425794 248 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 248 22 annotation annotation NN 10_1101-2021_01_07_425794 248 23 . . . 10_1101-2021_01_07_425794 249 1 We -PRON- PRP 10_1101-2021_01_07_425794 249 2 compared compare VBD 10_1101-2021_01_07_425794 249 3 the the DT 10_1101-2021_01_07_425794 249 4 recent recent JJ 10_1101-2021_01_07_425794 249 5 and and CC 10_1101-2021_01_07_425794 249 6 old old JJ 10_1101-2021_01_07_425794 249 7 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 249 8 annotations annotation NNS 10_1101-2021_01_07_425794 249 9 to to TO 10_1101-2021_01_07_425794 249 10 assess assess VB 10_1101-2021_01_07_425794 249 11 the the DT 10_1101-2021_01_07_425794 249 12 quality quality NN 10_1101-2021_01_07_425794 249 13 of of IN 10_1101-2021_01_07_425794 249 14 the the DT 10_1101-2021_01_07_425794 249 15 new new JJ 10_1101-2021_01_07_425794 249 16 annotations annotation NNS 10_1101-2021_01_07_425794 249 17 that that WDT 10_1101-2021_01_07_425794 249 18 were be VBD 10_1101-2021_01_07_425794 249 19 added add VBN 10_1101-2021_01_07_425794 249 20 when when WRB 10_1101-2021_01_07_425794 249 21 the the DT 10_1101-2021_01_07_425794 249 22 sequencing sequencing NN 10_1101-2021_01_07_425794 249 23 technology technology NN 10_1101-2021_01_07_425794 249 24 was be VBD 10_1101-2021_01_07_425794 249 25 utilized utilize VBN 10_1101-2021_01_07_425794 249 26 at at IN 10_1101-2021_01_07_425794 249 27 NCBI NCBI NNP 10_1101-2021_01_07_425794 249 28 for for IN 10_1101-2021_01_07_425794 249 29 curating curate VBG 10_1101-2021_01_07_425794 249 30 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 249 31 gene gene NN 10_1101-2021_01_07_425794 249 32 annotations annotation NNS 10_1101-2021_01_07_425794 249 33 . . . 10_1101-2021_01_07_425794 250 1 Although although IN 10_1101-2021_01_07_425794 250 2 the the DT 10_1101-2021_01_07_425794 250 3 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 250 4 annotation annotation NN 10_1101-2021_01_07_425794 250 5 contains contain VBZ 10_1101-2021_01_07_425794 250 6 significantly significantly RB 10_1101-2021_01_07_425794 250 7 more more JJR 10_1101-2021_01_07_425794 250 8 genes gene NNS 10_1101-2021_01_07_425794 250 9 than than IN 10_1101-2021_01_07_425794 250 10 both both DT 10_1101-2021_01_07_425794 250 11 the the DT 10_1101-2021_01_07_425794 250 12 recent recent JJ 10_1101-2021_01_07_425794 250 13 and and CC 10_1101-2021_01_07_425794 250 14 old old JJ 10_1101-2021_01_07_425794 250 15 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 250 16 annotations annotation NNS 10_1101-2021_01_07_425794 250 17 , , , 10_1101-2021_01_07_425794 250 18 it -PRON- PRP 10_1101-2021_01_07_425794 250 19 was be VBD 10_1101-2021_01_07_425794 250 20 also also RB 10_1101-2021_01_07_425794 250 21 found find VBN 10_1101-2021_01_07_425794 250 22 to to TO 10_1101-2021_01_07_425794 250 23 have have VB 10_1101-2021_01_07_425794 250 24 a a DT 10_1101-2021_01_07_425794 250 25 much much RB 10_1101-2021_01_07_425794 250 26 higher high JJR 10_1101-2021_01_07_425794 250 27 proportion proportion NN 10_1101-2021_01_07_425794 250 28 of of IN 10_1101-2021_01_07_425794 250 29 short short JJ 10_1101-2021_01_07_425794 250 30 genes gene NNS 10_1101-2021_01_07_425794 250 31 . . . 10_1101-2021_01_07_425794 251 1 Interestingly interestingly RB 10_1101-2021_01_07_425794 251 2 , , , 10_1101-2021_01_07_425794 251 3 we -PRON- PRP 10_1101-2021_01_07_425794 251 4 found find VBD 10_1101-2021_01_07_425794 251 5 that that IN 10_1101-2021_01_07_425794 251 6 a a DT 10_1101-2021_01_07_425794 251 7 much much RB 10_1101-2021_01_07_425794 251 8 higher high JJR 10_1101-2021_01_07_425794 251 9 fraction fraction NN 10_1101-2021_01_07_425794 251 10 of of IN 10_1101-2021_01_07_425794 251 11 these these DT 10_1101-2021_01_07_425794 251 12 short short JJ 10_1101-2021_01_07_425794 251 13 genes gene NNS 10_1101-2021_01_07_425794 251 14 in in IN 10_1101-2021_01_07_425794 251 15 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 251 16 were be VBD 10_1101-2021_01_07_425794 251 17 filtered filter VBN 10_1101-2021_01_07_425794 251 18 out out RP 10_1101-2021_01_07_425794 251 19 due due IN 10_1101-2021_01_07_425794 251 20 to to IN 10_1101-2021_01_07_425794 251 21 low low JJ 10_1101-2021_01_07_425794 251 22 or or CC 10_1101-2021_01_07_425794 251 23 no no DT 10_1101-2021_01_07_425794 251 24 expression expression NN 10_1101-2021_01_07_425794 251 25 in in IN 10_1101-2021_01_07_425794 251 26 the the DT 10_1101-2021_01_07_425794 251 27 analysis analysis NN 10_1101-2021_01_07_425794 251 28 of of IN 10_1101-2021_01_07_425794 251 29 the the DT 10_1101-2021_01_07_425794 251 30 SEQC SEQC NNP 10_1101-2021_01_07_425794 251 31 RNA- RNA- NNP 10_1101-2021_01_07_425794 251 32 seq seq NN 10_1101-2021_01_07_425794 251 33 dataset dataset NN 10_1101-2021_01_07_425794 251 34 , , , 10_1101-2021_01_07_425794 251 35 compared compare VBN 10_1101-2021_01_07_425794 251 36 to to IN 10_1101-2021_01_07_425794 251 37 the the DT 10_1101-2021_01_07_425794 251 38 short short JJ 10_1101-2021_01_07_425794 251 39 genes gene NNS 10_1101-2021_01_07_425794 251 40 included include VBN 10_1101-2021_01_07_425794 251 41 in in IN 10_1101-2021_01_07_425794 251 42 the the DT 10_1101-2021_01_07_425794 251 43 two two CD 10_1101-2021_01_07_425794 251 44 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 251 45 annotations annotation NNS 10_1101-2021_01_07_425794 251 46 . . . 10_1101-2021_01_07_425794 252 1 The the DT 10_1101-2021_01_07_425794 252 2 SEQC SEQC NNP 10_1101-2021_01_07_425794 252 3 RNA RNA NNP 10_1101-2021_01_07_425794 252 4 - - HYPH 10_1101-2021_01_07_425794 252 5 seq seq NN 10_1101-2021_01_07_425794 252 6 data datum NNS 10_1101-2021_01_07_425794 252 7 is be VBZ 10_1101-2021_01_07_425794 252 8 a a DT 10_1101-2021_01_07_425794 252 9 widely widely RB 10_1101-2021_01_07_425794 252 10 used use VBN 10_1101-2021_01_07_425794 252 11 benchmark benchmark JJ 10_1101-2021_01_07_425794 252 12 dataset dataset NN 10_1101-2021_01_07_425794 252 13 including include VBG 10_1101-2021_01_07_425794 252 14 the the DT 10_1101-2021_01_07_425794 252 15 Human Human NNP 10_1101-2021_01_07_425794 252 16 Brain Brain NNP 10_1101-2021_01_07_425794 252 17 Reference Reference NNP 10_1101-2021_01_07_425794 252 18 RNA RNA NNP 10_1101-2021_01_07_425794 252 19 and and CC 10_1101-2021_01_07_425794 252 20 Universal Universal NNP 10_1101-2021_01_07_425794 252 21 Human Human NNP 10_1101-2021_01_07_425794 252 22 Reference Reference NNP 10_1101-2021_01_07_425794 252 23 RNA RNA NNP 10_1101-2021_01_07_425794 252 24 samples sample NNS 10_1101-2021_01_07_425794 252 25 , , , 10_1101-2021_01_07_425794 252 26 in in IN 10_1101-2021_01_07_425794 252 27 which which WDT 10_1101-2021_01_07_425794 252 28 a a DT 10_1101-2021_01_07_425794 252 29 very very RB 10_1101-2021_01_07_425794 252 30 large large JJ 10_1101-2021_01_07_425794 252 31 number number NN 10_1101-2021_01_07_425794 252 32 of of IN 10_1101-2021_01_07_425794 252 33 gene gene NN 10_1101-2021_01_07_425794 252 34 expressed express VBD 10_1101-2021_01_07_425794 252 35 making make VBG 10_1101-2021_01_07_425794 252 36 the the DT 10_1101-2021_01_07_425794 252 37 entire entire JJ 10_1101-2021_01_07_425794 252 38 human human NN 10_1101-2021_01_07_425794 252 39 transcriptome transcriptome RBS 10_1101-2021_01_07_425794 252 40 well well RB 10_1101-2021_01_07_425794 252 41 covered cover VBN 10_1101-2021_01_07_425794 252 42 . . . 10_1101-2021_01_07_425794 253 1 The the DT 10_1101-2021_01_07_425794 253 2 use use NN 10_1101-2021_01_07_425794 253 3 of of IN 10_1101-2021_01_07_425794 253 4 the the DT 10_1101-2021_01_07_425794 253 5 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 253 6 - - HYPH 10_1101-2021_01_07_425794 253 7 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 253 8 annotation annotation NN 10_1101-2021_01_07_425794 253 9 ( ( -LRB- 10_1101-2021_01_07_425794 253 10 the the DT 10_1101-2021_01_07_425794 253 11 older old JJR 10_1101-2021_01_07_425794 253 12 version version NN 10_1101-2021_01_07_425794 253 13 of of IN 10_1101-2021_01_07_425794 253 14 the the DT 10_1101-2021_01_07_425794 253 15 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 253 16 anno- anno- JJ 10_1101-2021_01_07_425794 253 17 tation tation NN 10_1101-2021_01_07_425794 253 18 used use VBN 10_1101-2021_01_07_425794 253 19 in in IN 10_1101-2021_01_07_425794 253 20 this this DT 10_1101-2021_01_07_425794 253 21 study study NN 10_1101-2021_01_07_425794 253 22 ) ) -RRB- 10_1101-2021_01_07_425794 253 23 has have VBZ 10_1101-2021_01_07_425794 253 24 led lead VBN 10_1101-2021_01_07_425794 253 25 to to IN 10_1101-2021_01_07_425794 253 26 substantially substantially RB 10_1101-2021_01_07_425794 253 27 more more JJR 10_1101-2021_01_07_425794 253 28 fragments fragment NNS 10_1101-2021_01_07_425794 253 29 being be VBG 10_1101-2021_01_07_425794 253 30 successfully successfully RB 10_1101-2021_01_07_425794 253 31 counted count VBN 10_1101-2021_01_07_425794 253 32 to to IN 10_1101-2021_01_07_425794 253 33 genes gene NNS 10_1101-2021_01_07_425794 253 34 than than IN 10_1101-2021_01_07_425794 253 35 the the DT 10_1101-2021_01_07_425794 253 36 use use NN 10_1101-2021_01_07_425794 253 37 of of IN 10_1101-2021_01_07_425794 253 38 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 253 39 - - HYPH 10_1101-2021_01_07_425794 253 40 NCBI NCBI NNP 10_1101-2021_01_07_425794 253 41 ( ( -LRB- 10_1101-2021_01_07_425794 253 42 the the DT 10_1101-2021_01_07_425794 253 43 recent recent JJ 10_1101-2021_01_07_425794 253 44 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 253 45 annotation annotation NN 10_1101-2021_01_07_425794 253 46 used use VBN 10_1101-2021_01_07_425794 253 47 in in IN 10_1101-2021_01_07_425794 253 48 this this DT 10_1101-2021_01_07_425794 253 49 study study NN 10_1101-2021_01_07_425794 253 50 ) ) -RRB- 10_1101-2021_01_07_425794 253 51 or or CC 10_1101-2021_01_07_425794 253 52 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 253 53 annotations annotation NNS 10_1101-2021_01_07_425794 253 54 . . . 10_1101-2021_01_07_425794 254 1 A a DT 10_1101-2021_01_07_425794 254 2 detailed detailed JJ 10_1101-2021_01_07_425794 254 3 investigation investigation NN 10_1101-2021_01_07_425794 254 4 revealed reveal VBD 10_1101-2021_01_07_425794 254 5 that that IN 10_1101-2021_01_07_425794 254 6 this this DT 10_1101-2021_01_07_425794 254 7 was be VBD 10_1101-2021_01_07_425794 254 8 be- be- XX 10_1101-2021_01_07_425794 254 9 cause cause NN 10_1101-2021_01_07_425794 254 10 ( ( -LRB- 10_1101-2021_01_07_425794 254 11 a a LS 10_1101-2021_01_07_425794 254 12 ) ) -RRB- 10_1101-2021_01_07_425794 254 13 there there EX 10_1101-2021_01_07_425794 254 14 are be VBP 10_1101-2021_01_07_425794 254 15 less less RBR 10_1101-2021_01_07_425794 254 16 overlapping overlap VBG 10_1101-2021_01_07_425794 254 17 between between IN 10_1101-2021_01_07_425794 254 18 genes gene NNS 10_1101-2021_01_07_425794 254 19 in in IN 10_1101-2021_01_07_425794 254 20 the the DT 10_1101-2021_01_07_425794 254 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 254 22 - - HYPH 10_1101-2021_01_07_425794 254 23 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 254 24 annotation annotation NN 10_1101-2021_01_07_425794 254 25 leading lead VBG 10_1101-2021_01_07_425794 254 26 to to IN 10_1101-2021_01_07_425794 254 27 less less RBR 10_1101-2021_01_07_425794 254 28 read read VB 10_1101-2021_01_07_425794 254 29 assignment assignment JJ 10_1101-2021_01_07_425794 254 30 ambiguity ambiguity NN 10_1101-2021_01_07_425794 254 31 and and CC 10_1101-2021_01_07_425794 254 32 ( ( -LRB- 10_1101-2021_01_07_425794 254 33 b b LS 10_1101-2021_01_07_425794 254 34 ) ) -RRB- 10_1101-2021_01_07_425794 254 35 the the DT 10_1101-2021_01_07_425794 254 36 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 254 37 - - HYPH 10_1101-2021_01_07_425794 254 38 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 254 39 annotation annotation NN 10_1101-2021_01_07_425794 254 40 con- con- NN 10_1101-2021_01_07_425794 254 41 tains tain VBZ 10_1101-2021_01_07_425794 254 42 more more JJR 10_1101-2021_01_07_425794 254 43 genes gene NNS 10_1101-2021_01_07_425794 254 44 that that WDT 10_1101-2021_01_07_425794 254 45 are be VBP 10_1101-2021_01_07_425794 254 46 compatible compatible JJ 10_1101-2021_01_07_425794 254 47 with with IN 10_1101-2021_01_07_425794 254 48 mapped map VBN 10_1101-2021_01_07_425794 254 49 fragments fragment NNS 10_1101-2021_01_07_425794 254 50 , , , 10_1101-2021_01_07_425794 254 51 despite despite IN 10_1101-2021_01_07_425794 254 52 the the DT 10_1101-2021_01_07_425794 254 53 transcriptome transcriptome DT 10_1101-2021_01_07_425794 254 54 represented represent VBN 10_1101-2021_01_07_425794 254 55 by by IN 10_1101-2021_01_07_425794 254 56 this this DT 10_1101-2021_01_07_425794 254 57 annotation annotation NN 10_1101-2021_01_07_425794 254 58 is be VBZ 10_1101-2021_01_07_425794 254 59 much much RB 10_1101-2021_01_07_425794 254 60 smaller small JJR 10_1101-2021_01_07_425794 254 61 than than IN 10_1101-2021_01_07_425794 254 62 those those DT 10_1101-2021_01_07_425794 254 63 represented represent VBN 10_1101-2021_01_07_425794 254 64 by by IN 10_1101-2021_01_07_425794 254 65 the the DT 10_1101-2021_01_07_425794 254 66 RefSeq- RefSeq- NNP 10_1101-2021_01_07_425794 254 67 NCBI NCBI NNP 10_1101-2021_01_07_425794 254 68 and and CC 10_1101-2021_01_07_425794 254 69 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 254 70 annotations annotation NNS 10_1101-2021_01_07_425794 254 71 . . . 10_1101-2021_01_07_425794 255 1 Moreover moreover RB 10_1101-2021_01_07_425794 255 2 , , , 10_1101-2021_01_07_425794 255 3 the the DT 10_1101-2021_01_07_425794 255 4 quantification quantification NN 10_1101-2021_01_07_425794 255 5 data datum NNS 10_1101-2021_01_07_425794 255 6 obtained obtain VBN 10_1101-2021_01_07_425794 255 7 from from IN 10_1101-2021_01_07_425794 255 8 using use VBG 10_1101-2021_01_07_425794 255 9 19 19 CD 10_1101-2021_01_07_425794 255 10 .CC .CC , 10_1101-2021_01_07_425794 255 11 - - HYPH 10_1101-2021_01_07_425794 255 12 BY by IN 10_1101-2021_01_07_425794 255 13 - - HYPH 10_1101-2021_01_07_425794 255 14 NC NC NNP 10_1101-2021_01_07_425794 255 15 - - HYPH 10_1101-2021_01_07_425794 255 16 ND ND NNP 10_1101-2021_01_07_425794 255 17 4.0 4.0 CD 10_1101-2021_01_07_425794 255 18 International international JJ 10_1101-2021_01_07_425794 255 19 licensemade licensemade NN 10_1101-2021_01_07_425794 255 20 available available JJ 10_1101-2021_01_07_425794 255 21 under under IN 10_1101-2021_01_07_425794 255 22 a a DT 10_1101-2021_01_07_425794 255 23 ( ( -LRB- 10_1101-2021_01_07_425794 255 24 which which WDT 10_1101-2021_01_07_425794 255 25 was be VBD 10_1101-2021_01_07_425794 255 26 not not RB 10_1101-2021_01_07_425794 255 27 certified certify VBN 10_1101-2021_01_07_425794 255 28 by by IN 10_1101-2021_01_07_425794 255 29 peer peer NN 10_1101-2021_01_07_425794 255 30 review review NN 10_1101-2021_01_07_425794 255 31 ) ) -RRB- 10_1101-2021_01_07_425794 255 32 is be VBZ 10_1101-2021_01_07_425794 255 33 the the DT 10_1101-2021_01_07_425794 255 34 author author NN 10_1101-2021_01_07_425794 255 35 / / SYM 10_1101-2021_01_07_425794 255 36 funder funder NN 10_1101-2021_01_07_425794 255 37 , , , 10_1101-2021_01_07_425794 255 38 who who WP 10_1101-2021_01_07_425794 255 39 has have VBZ 10_1101-2021_01_07_425794 255 40 granted grant VBN 10_1101-2021_01_07_425794 255 41 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 255 42 a a DT 10_1101-2021_01_07_425794 255 43 license license NN 10_1101-2021_01_07_425794 255 44 to to TO 10_1101-2021_01_07_425794 255 45 display display VB 10_1101-2021_01_07_425794 255 46 the the DT 10_1101-2021_01_07_425794 255 47 preprint preprint NN 10_1101-2021_01_07_425794 255 48 in in IN 10_1101-2021_01_07_425794 255 49 perpetuity perpetuity NN 10_1101-2021_01_07_425794 255 50 . . . 10_1101-2021_01_07_425794 256 1 It -PRON- PRP 10_1101-2021_01_07_425794 256 2 is be VBZ 10_1101-2021_01_07_425794 256 3 The the DT 10_1101-2021_01_07_425794 256 4 copyright copyright NN 10_1101-2021_01_07_425794 256 5 holder holder NN 10_1101-2021_01_07_425794 256 6 for for IN 10_1101-2021_01_07_425794 256 7 this this DT 10_1101-2021_01_07_425794 256 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 256 9 version version NN 10_1101-2021_01_07_425794 256 10 posted post VBD 10_1101-2021_01_07_425794 256 11 January January NNP 10_1101-2021_01_07_425794 256 12 8 8 CD 10_1101-2021_01_07_425794 256 13 , , , 10_1101-2021_01_07_425794 256 14 2021 2021 CD 10_1101-2021_01_07_425794 256 15 . . . 10_1101-2021_01_07_425794 256 16 ; ; : 10_1101-2021_01_07_425794 256 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 256 18 : : : 10_1101-2021_01_07_425794 256 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 256 20 preprint preprint JJ 10_1101-2021_01_07_425794 256 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 256 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ CD 10_1101-2021_01_07_425794 256 23 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 256 24 - - HYPH 10_1101-2021_01_07_425794 256 25 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 256 26 exhibited exhibit VBD 10_1101-2021_01_07_425794 256 27 desirable desirable JJ 10_1101-2021_01_07_425794 256 28 larger large JJR 10_1101-2021_01_07_425794 256 29 intensity intensity NN 10_1101-2021_01_07_425794 256 30 range range NN 10_1101-2021_01_07_425794 256 31 and and CC 10_1101-2021_01_07_425794 256 32 higher high JJR 10_1101-2021_01_07_425794 256 33 median median JJ 10_1101-2021_01_07_425794 256 34 expression expression NN 10_1101-2021_01_07_425794 256 35 level level NN 10_1101-2021_01_07_425794 256 36 than than IN 10_1101-2021_01_07_425794 256 37 the the DT 10_1101-2021_01_07_425794 256 38 quantification quantification NN 10_1101-2021_01_07_425794 256 39 data datum NNS 10_1101-2021_01_07_425794 256 40 obtained obtain VBN 10_1101-2021_01_07_425794 256 41 from from IN 10_1101-2021_01_07_425794 256 42 using use VBG 10_1101-2021_01_07_425794 256 43 the the DT 10_1101-2021_01_07_425794 256 44 other other JJ 10_1101-2021_01_07_425794 256 45 two two CD 10_1101-2021_01_07_425794 256 46 annotations annotation NNS 10_1101-2021_01_07_425794 256 47 . . . 10_1101-2021_01_07_425794 257 1 The the DT 10_1101-2021_01_07_425794 257 2 evaluation evaluation NN 10_1101-2021_01_07_425794 257 3 of of IN 10_1101-2021_01_07_425794 257 4 quantification quantification NN 10_1101-2021_01_07_425794 257 5 accuracy accuracy NN 10_1101-2021_01_07_425794 257 6 from from IN 10_1101-2021_01_07_425794 257 7 using use VBG 10_1101-2021_01_07_425794 257 8 genome genome NN 10_1101-2021_01_07_425794 257 9 - - HYPH 10_1101-2021_01_07_425794 257 10 wide wide JJ 10_1101-2021_01_07_425794 257 11 titration titration NN 10_1101-2021_01_07_425794 257 12 mono- mono- NN 10_1101-2021_01_07_425794 257 13 tonicity tonicity NN 10_1101-2021_01_07_425794 257 14 truth truth NN 10_1101-2021_01_07_425794 257 15 built build VBN 10_1101-2021_01_07_425794 257 16 in in IN 10_1101-2021_01_07_425794 257 17 the the DT 10_1101-2021_01_07_425794 257 18 RNA RNA NNP 10_1101-2021_01_07_425794 257 19 - - HYPH 10_1101-2021_01_07_425794 257 20 seq seq NN 10_1101-2021_01_07_425794 257 21 data datum NNS 10_1101-2021_01_07_425794 257 22 , , , 10_1101-2021_01_07_425794 257 23 the the DT 10_1101-2021_01_07_425794 257 24 TaqMan TaqMan NNP 10_1101-2021_01_07_425794 257 25 RT RT NNP 10_1101-2021_01_07_425794 257 26 - - HYPH 10_1101-2021_01_07_425794 257 27 PCR PCR NNP 10_1101-2021_01_07_425794 257 28 data datum NNS 10_1101-2021_01_07_425794 257 29 and and CC 10_1101-2021_01_07_425794 257 30 the the DT 10_1101-2021_01_07_425794 257 31 microarray microarray NNP 10_1101-2021_01_07_425794 257 32 data datum NNS 10_1101-2021_01_07_425794 257 33 , , , 10_1101-2021_01_07_425794 257 34 showed show VBD 10_1101-2021_01_07_425794 257 35 that that IN 10_1101-2021_01_07_425794 257 36 overall overall PDT 10_1101-2021_01_07_425794 257 37 the the DT 10_1101-2021_01_07_425794 257 38 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 257 39 - - HYPH 10_1101-2021_01_07_425794 257 40 NCBI NCBI NNP 10_1101-2021_01_07_425794 257 41 annotation annotation NN 10_1101-2021_01_07_425794 257 42 yielded yield VBD 10_1101-2021_01_07_425794 257 43 better well JJR 10_1101-2021_01_07_425794 257 44 quantification quantification NN 10_1101-2021_01_07_425794 257 45 re- re- JJ 10_1101-2021_01_07_425794 257 46 sults sult NNS 10_1101-2021_01_07_425794 257 47 than than IN 10_1101-2021_01_07_425794 257 48 the the DT 10_1101-2021_01_07_425794 257 49 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 257 50 annotation annotation NN 10_1101-2021_01_07_425794 257 51 . . . 10_1101-2021_01_07_425794 258 1 This this DT 10_1101-2021_01_07_425794 258 2 may may MD 10_1101-2021_01_07_425794 258 3 not not RB 10_1101-2021_01_07_425794 258 4 be be VB 10_1101-2021_01_07_425794 258 5 surprising surprising JJ 10_1101-2021_01_07_425794 258 6 because because IN 10_1101-2021_01_07_425794 258 7 the the DT 10_1101-2021_01_07_425794 258 8 NCBI NCBI NNP 10_1101-2021_01_07_425794 258 9 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 258 10 annotation annotation NN 10_1101-2021_01_07_425794 258 11 is be VBZ 10_1101-2021_01_07_425794 258 12 a a DT 10_1101-2021_01_07_425794 258 13 traditionally traditionally RB 10_1101-2021_01_07_425794 258 14 conservative conservative JJ 10_1101-2021_01_07_425794 258 15 annotation annotation NN 10_1101-2021_01_07_425794 258 16 that that WDT 10_1101-2021_01_07_425794 258 17 is be VBZ 10_1101-2021_01_07_425794 258 18 known know VBN 10_1101-2021_01_07_425794 258 19 to to TO 10_1101-2021_01_07_425794 258 20 be be VB 10_1101-2021_01_07_425794 258 21 highly highly RB 10_1101-2021_01_07_425794 258 22 accurate accurate JJ 10_1101-2021_01_07_425794 258 23 as as IN 10_1101-2021_01_07_425794 258 24 it -PRON- PRP 10_1101-2021_01_07_425794 258 25 uses use VBZ 10_1101-2021_01_07_425794 258 26 an an DT 10_1101-2021_01_07_425794 258 27 evidence evidence NN 10_1101-2021_01_07_425794 258 28 - - HYPH 10_1101-2021_01_07_425794 258 29 based base VBN 10_1101-2021_01_07_425794 258 30 approach approach NN 10_1101-2021_01_07_425794 258 31 to to IN 10_1101-2021_01_07_425794 258 32 annotate annotate JJ 10_1101-2021_01_07_425794 258 33 genes gene NNS 10_1101-2021_01_07_425794 258 34 . . . 10_1101-2021_01_07_425794 259 1 However however RB 10_1101-2021_01_07_425794 259 2 , , , 10_1101-2021_01_07_425794 259 3 we -PRON- PRP 10_1101-2021_01_07_425794 259 4 also also RB 10_1101-2021_01_07_425794 259 5 found find VBD 10_1101-2021_01_07_425794 259 6 that that IN 10_1101-2021_01_07_425794 259 7 the the DT 10_1101-2021_01_07_425794 259 8 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 259 9 - - HYPH 10_1101-2021_01_07_425794 259 10 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 259 11 annotation annotation NN 10_1101-2021_01_07_425794 259 12 yielded yield VBD 10_1101-2021_01_07_425794 259 13 more more RBR 10_1101-2021_01_07_425794 259 14 accurate accurate JJ 10_1101-2021_01_07_425794 259 15 quantification quantification NN 10_1101-2021_01_07_425794 259 16 results result NNS 10_1101-2021_01_07_425794 259 17 than than IN 10_1101-2021_01_07_425794 259 18 the the DT 10_1101-2021_01_07_425794 259 19 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 259 20 - - HYPH 10_1101-2021_01_07_425794 259 21 NCBI NCBI NNP 10_1101-2021_01_07_425794 259 22 annotation annotation NN 10_1101-2021_01_07_425794 259 23 in in IN 10_1101-2021_01_07_425794 259 24 almost almost RB 10_1101-2021_01_07_425794 259 25 all all PDT 10_1101-2021_01_07_425794 259 26 the the DT 10_1101-2021_01_07_425794 259 27 comparisons comparison NNS 10_1101-2021_01_07_425794 259 28 , , , 10_1101-2021_01_07_425794 259 29 which which WDT 10_1101-2021_01_07_425794 259 30 is be VBZ 10_1101-2021_01_07_425794 259 31 very very RB 10_1101-2021_01_07_425794 259 32 surprising surprising JJ 10_1101-2021_01_07_425794 259 33 . . . 10_1101-2021_01_07_425794 260 1 We -PRON- PRP 10_1101-2021_01_07_425794 260 2 suspect suspect VBP 10_1101-2021_01_07_425794 260 3 that that IN 10_1101-2021_01_07_425794 260 4 this this DT 10_1101-2021_01_07_425794 260 5 might may MD 10_1101-2021_01_07_425794 260 6 be be VB 10_1101-2021_01_07_425794 260 7 due due IN 10_1101-2021_01_07_425794 260 8 to to IN 10_1101-2021_01_07_425794 260 9 the the DT 10_1101-2021_01_07_425794 260 10 annotation annotation NN 10_1101-2021_01_07_425794 260 11 errors error NNS 10_1101-2021_01_07_425794 260 12 arising arise VBG 10_1101-2021_01_07_425794 260 13 from from IN 10_1101-2021_01_07_425794 260 14 the the DT 10_1101-2021_01_07_425794 260 15 sequencing sequence VBG 10_1101-2021_01_07_425794 260 16 data datum NNS 10_1101-2021_01_07_425794 260 17 recently recently RB 10_1101-2021_01_07_425794 260 18 utilized utilize VBN 10_1101-2021_01_07_425794 260 19 in in IN 10_1101-2021_01_07_425794 260 20 the the DT 10_1101-2021_01_07_425794 260 21 NCBI NCBI NNP 10_1101-2021_01_07_425794 260 22 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 260 23 annotation annotation NN 10_1101-2021_01_07_425794 260 24 generation generation NN 10_1101-2021_01_07_425794 260 25 pipeline pipeline NN 10_1101-2021_01_07_425794 260 26 . . . 10_1101-2021_01_07_425794 261 1 It -PRON- PRP 10_1101-2021_01_07_425794 261 2 was be VBD 10_1101-2021_01_07_425794 261 3 reported report VBN 10_1101-2021_01_07_425794 261 4 that that IN 10_1101-2021_01_07_425794 261 5 the the DT 10_1101-2021_01_07_425794 261 6 sequencing sequencing NN 10_1101-2021_01_07_425794 261 7 data datum NNS 10_1101-2021_01_07_425794 261 8 , , , 10_1101-2021_01_07_425794 261 9 including include VBG 10_1101-2021_01_07_425794 261 10 RNA RNA NNP 10_1101-2021_01_07_425794 261 11 - - HYPH 10_1101-2021_01_07_425794 261 12 seq seq NN 10_1101-2021_01_07_425794 261 13 data datum NNS 10_1101-2021_01_07_425794 261 14 and and CC 10_1101-2021_01_07_425794 261 15 epigenome epigenome JJ 10_1101-2021_01_07_425794 261 16 sequencing sequencing NN 10_1101-2021_01_07_425794 261 17 data datum NNS 10_1101-2021_01_07_425794 261 18 , , , 10_1101-2021_01_07_425794 261 19 started start VBD 10_1101-2021_01_07_425794 261 20 to to TO 10_1101-2021_01_07_425794 261 21 be be VB 10_1101-2021_01_07_425794 261 22 utilized utilize VBN 10_1101-2021_01_07_425794 261 23 by by IN 10_1101-2021_01_07_425794 261 24 NCBI NCBI NNP 10_1101-2021_01_07_425794 261 25 for for IN 10_1101-2021_01_07_425794 261 26 curating curate VBG 10_1101-2021_01_07_425794 261 27 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 261 28 gene gene NN 10_1101-2021_01_07_425794 261 29 annotations annotation NNS 10_1101-2021_01_07_425794 261 30 in in IN 10_1101-2021_01_07_425794 261 31 around around IN 10_1101-2021_01_07_425794 261 32 2013 2013 CD 10_1101-2021_01_07_425794 261 33 [ [ -LRB- 10_1101-2021_01_07_425794 261 34 9 9 CD 10_1101-2021_01_07_425794 261 35 , , , 10_1101-2021_01_07_425794 261 36 27 27 CD 10_1101-2021_01_07_425794 261 37 ] ] -RRB- 10_1101-2021_01_07_425794 261 38 . . . 10_1101-2021_01_07_425794 262 1 Between between IN 10_1101-2021_01_07_425794 262 2 March March NNP 10_1101-2021_01_07_425794 262 3 2015 2015 CD 10_1101-2021_01_07_425794 262 4 and and CC 10_1101-2021_01_07_425794 262 5 July July NNP 10_1101-2021_01_07_425794 262 6 2020 2020 CD 10_1101-2021_01_07_425794 262 7 , , , 10_1101-2021_01_07_425794 262 8 the the DT 10_1101-2021_01_07_425794 262 9 number number NN 10_1101-2021_01_07_425794 262 10 of of IN 10_1101-2021_01_07_425794 262 11 gene gene NN 10_1101-2021_01_07_425794 262 12 transcripts transcript NNS 10_1101-2021_01_07_425794 262 13 in in IN 10_1101-2021_01_07_425794 262 14 the the DT 10_1101-2021_01_07_425794 262 15 vertebrate vertebrate JJ 10_1101-2021_01_07_425794 262 16 mammalian mammalian JJ 10_1101-2021_01_07_425794 262 17 organisms organism NNS 10_1101-2021_01_07_425794 262 18 included include VBN 10_1101-2021_01_07_425794 262 19 in in IN 10_1101-2021_01_07_425794 262 20 the the DT 10_1101-2021_01_07_425794 262 21 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 262 22 database database NN 10_1101-2021_01_07_425794 262 23 increased increase VBD 10_1101-2021_01_07_425794 262 24 significantly significantly RB 10_1101-2021_01_07_425794 262 25 from from IN 10_1101-2021_01_07_425794 262 26 3.6 3.6 CD 10_1101-2021_01_07_425794 262 27 million million CD 10_1101-2021_01_07_425794 262 28 to to TO 10_1101-2021_01_07_425794 262 29 7.8 7.8 CD 10_1101-2021_01_07_425794 262 30 million million CD 10_1101-2021_01_07_425794 262 31 ( ( -LRB- 10_1101-2021_01_07_425794 262 32 https://www.ncbi.nlm.nih.gov/refseq/statistics/ https://www.ncbi.nlm.nih.gov/refseq/statistics/ NNP 10_1101-2021_01_07_425794 262 33 ) ) -RRB- 10_1101-2021_01_07_425794 262 34 , , , 10_1101-2021_01_07_425794 262 35 a a DT 10_1101-2021_01_07_425794 262 36 more more RBR 10_1101-2021_01_07_425794 262 37 than than IN 10_1101-2021_01_07_425794 262 38 twofold twofold JJ 10_1101-2021_01_07_425794 262 39 increase increase NN 10_1101-2021_01_07_425794 262 40 in in IN 10_1101-2021_01_07_425794 262 41 just just RB 10_1101-2021_01_07_425794 262 42 around around IN 10_1101-2021_01_07_425794 262 43 5 5 CD 10_1101-2021_01_07_425794 262 44 years year NNS 10_1101-2021_01_07_425794 262 45 . . . 10_1101-2021_01_07_425794 263 1 The the DT 10_1101-2021_01_07_425794 263 2 use use NN 10_1101-2021_01_07_425794 263 3 of of IN 10_1101-2021_01_07_425794 263 4 sequencing sequence VBG 10_1101-2021_01_07_425794 263 5 data datum NNS 10_1101-2021_01_07_425794 263 6 for for IN 10_1101-2021_01_07_425794 263 7 annotation annotation NN 10_1101-2021_01_07_425794 263 8 generation generation NN 10_1101-2021_01_07_425794 263 9 should should MD 10_1101-2021_01_07_425794 263 10 be be VB 10_1101-2021_01_07_425794 263 11 a a DT 10_1101-2021_01_07_425794 263 12 significant significant JJ 10_1101-2021_01_07_425794 263 13 driver driver NN 10_1101-2021_01_07_425794 263 14 for for IN 10_1101-2021_01_07_425794 263 15 this this DT 10_1101-2021_01_07_425794 263 16 rapid rapid JJ 10_1101-2021_01_07_425794 263 17 expansion expansion NN 10_1101-2021_01_07_425794 263 18 of of IN 10_1101-2021_01_07_425794 263 19 the the DT 10_1101-2021_01_07_425794 263 20 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 263 21 database database NN 10_1101-2021_01_07_425794 263 22 . . . 10_1101-2021_01_07_425794 264 1 It -PRON- PRP 10_1101-2021_01_07_425794 264 2 is be VBZ 10_1101-2021_01_07_425794 264 3 known know VBN 10_1101-2021_01_07_425794 264 4 that that IN 10_1101-2021_01_07_425794 264 5 some some DT 10_1101-2021_01_07_425794 264 6 errors error NNS 10_1101-2021_01_07_425794 264 7 associated associate VBN 10_1101-2021_01_07_425794 264 8 with with IN 10_1101-2021_01_07_425794 264 9 the the DT 10_1101-2021_01_07_425794 264 10 generation generation NN 10_1101-2021_01_07_425794 264 11 and and CC 10_1101-2021_01_07_425794 264 12 analysis analysis NN 10_1101-2021_01_07_425794 264 13 of of IN 10_1101-2021_01_07_425794 264 14 sequencing sequence VBG 10_1101-2021_01_07_425794 264 15 data datum NNS 10_1101-2021_01_07_425794 264 16 are be VBP 10_1101-2021_01_07_425794 264 17 difficult difficult JJ 10_1101-2021_01_07_425794 264 18 to to TO 10_1101-2021_01_07_425794 264 19 correct correct VB 10_1101-2021_01_07_425794 264 20 , , , 10_1101-2021_01_07_425794 264 21 such such JJ 10_1101-2021_01_07_425794 264 22 as as IN 10_1101-2021_01_07_425794 264 23 sample sample NN 10_1101-2021_01_07_425794 264 24 contamination contamination NN 10_1101-2021_01_07_425794 264 25 , , , 10_1101-2021_01_07_425794 264 26 sequencing sequence VBG 10_1101-2021_01_07_425794 264 27 errors error NNS 10_1101-2021_01_07_425794 264 28 , , , 10_1101-2021_01_07_425794 264 29 read read VB 10_1101-2021_01_07_425794 264 30 mapping mapping NN 10_1101-2021_01_07_425794 264 31 errors error NNS 10_1101-2021_01_07_425794 264 32 and and CC 10_1101-2021_01_07_425794 264 33 read read VBD 10_1101-2021_01_07_425794 264 34 assembly assembly NN 10_1101-2021_01_07_425794 264 35 errors error NNS 10_1101-2021_01_07_425794 264 36 . . . 10_1101-2021_01_07_425794 265 1 When when WRB 10_1101-2021_01_07_425794 265 2 these these DT 10_1101-2021_01_07_425794 265 3 errors error NNS 10_1101-2021_01_07_425794 265 4 were be VBD 10_1101-2021_01_07_425794 265 5 brought bring VBN 10_1101-2021_01_07_425794 265 6 to to IN 10_1101-2021_01_07_425794 265 7 the the DT 10_1101-2021_01_07_425794 265 8 annotation annotation NN 10_1101-2021_01_07_425794 265 9 process process NN 10_1101-2021_01_07_425794 265 10 , , , 10_1101-2021_01_07_425794 265 11 they -PRON- PRP 10_1101-2021_01_07_425794 265 12 could could MD 10_1101-2021_01_07_425794 265 13 result result VB 10_1101-2021_01_07_425794 265 14 in in IN 10_1101-2021_01_07_425794 265 15 incorrect incorrect JJ 10_1101-2021_01_07_425794 265 16 gene gene NN 10_1101-2021_01_07_425794 265 17 annotations annotation NNS 10_1101-2021_01_07_425794 265 18 being be VBG 10_1101-2021_01_07_425794 265 19 generated generate VBN 10_1101-2021_01_07_425794 265 20 and and CC 10_1101-2021_01_07_425794 265 21 consequently consequently RB 10_1101-2021_01_07_425794 265 22 led lead VBD 10_1101-2021_01_07_425794 265 23 to to IN 10_1101-2021_01_07_425794 265 24 less less RBR 10_1101-2021_01_07_425794 265 25 accurate accurate JJ 10_1101-2021_01_07_425794 265 26 quantification quantification NN 10_1101-2021_01_07_425794 265 27 of of IN 10_1101-2021_01_07_425794 265 28 the the DT 10_1101-2021_01_07_425794 265 29 RNA RNA NNP 10_1101-2021_01_07_425794 265 30 - - HYPH 10_1101-2021_01_07_425794 265 31 seq seq NN 10_1101-2021_01_07_425794 265 32 data datum NNS 10_1101-2021_01_07_425794 265 33 . . . 10_1101-2021_01_07_425794 266 1 5 5 CD 10_1101-2021_01_07_425794 266 2 CONCLUSION conclusion NN 10_1101-2021_01_07_425794 266 3 In in IN 10_1101-2021_01_07_425794 266 4 conclusion conclusion NN 10_1101-2021_01_07_425794 266 5 , , , 10_1101-2021_01_07_425794 266 6 our -PRON- PRP$ 10_1101-2021_01_07_425794 266 7 findings finding NNS 10_1101-2021_01_07_425794 266 8 from from IN 10_1101-2021_01_07_425794 266 9 this this DT 10_1101-2021_01_07_425794 266 10 study study NN 10_1101-2021_01_07_425794 266 11 revealed reveal VBD 10_1101-2021_01_07_425794 266 12 that that IN 10_1101-2021_01_07_425794 266 13 the the DT 10_1101-2021_01_07_425794 266 14 NCBI NCBI NNP 10_1101-2021_01_07_425794 266 15 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 266 16 human human JJ 10_1101-2021_01_07_425794 266 17 gene gene NN 10_1101-2021_01_07_425794 266 18 annotations annotation NNS 10_1101-2021_01_07_425794 266 19 outperformed outperform VBD 10_1101-2021_01_07_425794 266 20 the the DT 10_1101-2021_01_07_425794 266 21 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 266 22 human human JJ 10_1101-2021_01_07_425794 266 23 gene gene NN 10_1101-2021_01_07_425794 266 24 annotation annotation NN 10_1101-2021_01_07_425794 266 25 in in IN 10_1101-2021_01_07_425794 266 26 the the DT 10_1101-2021_01_07_425794 266 27 quantification quantification NN 10_1101-2021_01_07_425794 266 28 of of IN 10_1101-2021_01_07_425794 266 29 RNA RNA NNP 10_1101-2021_01_07_425794 266 30 - - HYPH 10_1101-2021_01_07_425794 266 31 seq seq NN 10_1101-2021_01_07_425794 266 32 data datum NNS 10_1101-2021_01_07_425794 266 33 . . . 10_1101-2021_01_07_425794 267 1 However however RB 10_1101-2021_01_07_425794 267 2 , , , 10_1101-2021_01_07_425794 267 3 we -PRON- PRP 10_1101-2021_01_07_425794 267 4 also also RB 10_1101-2021_01_07_425794 267 5 raised raise VBD 10_1101-2021_01_07_425794 267 6 concerns concern NNS 10_1101-2021_01_07_425794 267 7 over over IN 10_1101-2021_01_07_425794 267 8 the the DT 10_1101-2021_01_07_425794 267 9 recent recent JJ 10_1101-2021_01_07_425794 267 10 changes change NNS 10_1101-2021_01_07_425794 267 11 made make VBN 10_1101-2021_01_07_425794 267 12 to to IN 10_1101-2021_01_07_425794 267 13 the the DT 10_1101-2021_01_07_425794 267 14 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 267 15 database database NN 10_1101-2021_01_07_425794 267 16 due due IN 10_1101-2021_01_07_425794 267 17 to to IN 10_1101-2021_01_07_425794 267 18 the the DT 10_1101-2021_01_07_425794 267 19 use use NN 10_1101-2021_01_07_425794 267 20 of of IN 10_1101-2021_01_07_425794 267 21 sequencing sequence VBG 10_1101-2021_01_07_425794 267 22 data datum NNS 10_1101-2021_01_07_425794 267 23 in in IN 10_1101-2021_01_07_425794 267 24 the the DT 10_1101-2021_01_07_425794 267 25 annotation annotation NN 10_1101-2021_01_07_425794 267 26 generation generation NN 10_1101-2021_01_07_425794 267 27 process process NN 10_1101-2021_01_07_425794 267 28 . . . 10_1101-2021_01_07_425794 268 1 These these DT 10_1101-2021_01_07_425794 268 2 changes change NNS 10_1101-2021_01_07_425794 268 3 need need VBP 10_1101-2021_01_07_425794 268 4 to to TO 10_1101-2021_01_07_425794 268 5 be be VB 10_1101-2021_01_07_425794 268 6 reviewed review VBN 10_1101-2021_01_07_425794 268 7 and and CC 10_1101-2021_01_07_425794 268 8 validated validate VBN 10_1101-2021_01_07_425794 268 9 so so IN 10_1101-2021_01_07_425794 268 10 as as IN 10_1101-2021_01_07_425794 268 11 to to TO 10_1101-2021_01_07_425794 268 12 ensure ensure VB 10_1101-2021_01_07_425794 268 13 the the DT 10_1101-2021_01_07_425794 268 14 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 268 15 database database NN 10_1101-2021_01_07_425794 268 16 continues continue VBZ 10_1101-2021_01_07_425794 268 17 to to TO 10_1101-2021_01_07_425794 268 18 be be VB 10_1101-2021_01_07_425794 268 19 a a DT 10_1101-2021_01_07_425794 268 20 reliable reliable JJ 10_1101-2021_01_07_425794 268 21 and and CC 10_1101-2021_01_07_425794 268 22 high high JJ 10_1101-2021_01_07_425794 268 23 - - HYPH 10_1101-2021_01_07_425794 268 24 quality quality NN 10_1101-2021_01_07_425794 268 25 gene gene NN 10_1101-2021_01_07_425794 268 26 annotation annotation NN 10_1101-2021_01_07_425794 268 27 resource resource NN 10_1101-2021_01_07_425794 268 28 for for IN 10_1101-2021_01_07_425794 268 29 the the DT 10_1101-2021_01_07_425794 268 30 research research NN 10_1101-2021_01_07_425794 268 31 com- com- NN 10_1101-2021_01_07_425794 268 32 munity munity NN 10_1101-2021_01_07_425794 268 33 . . . 10_1101-2021_01_07_425794 269 1 Similarly similarly RB 10_1101-2021_01_07_425794 269 2 , , , 10_1101-2021_01_07_425794 269 3 such such JJ 10_1101-2021_01_07_425794 269 4 review review NN 10_1101-2021_01_07_425794 269 5 should should MD 10_1101-2021_01_07_425794 269 6 be be VB 10_1101-2021_01_07_425794 269 7 conducted conduct VBN 10_1101-2021_01_07_425794 269 8 for for IN 10_1101-2021_01_07_425794 269 9 other other JJ 10_1101-2021_01_07_425794 269 10 gene gene NN 10_1101-2021_01_07_425794 269 11 annotation annotation NN 10_1101-2021_01_07_425794 269 12 databases database NNS 10_1101-2021_01_07_425794 269 13 as as RB 10_1101-2021_01_07_425794 269 14 well well RB 10_1101-2021_01_07_425794 269 15 . . . 10_1101-2021_01_07_425794 270 1 The the DT 10_1101-2021_01_07_425794 270 2 research research NN 10_1101-2021_01_07_425794 270 3 findings finding NNS 10_1101-2021_01_07_425794 270 4 from from IN 10_1101-2021_01_07_425794 270 5 this this DT 10_1101-2021_01_07_425794 270 6 study study NN 10_1101-2021_01_07_425794 270 7 also also RB 10_1101-2021_01_07_425794 270 8 have have VBP 10_1101-2021_01_07_425794 270 9 an an DT 10_1101-2021_01_07_425794 270 10 implication implication NN 10_1101-2021_01_07_425794 270 11 for for IN 10_1101-2021_01_07_425794 270 12 the the DT 10_1101-2021_01_07_425794 270 13 quantification quantification NN 10_1101-2021_01_07_425794 270 14 of of IN 10_1101-2021_01_07_425794 270 15 RNA RNA NNP 10_1101-2021_01_07_425794 270 16 - - HYPH 10_1101-2021_01_07_425794 270 17 seq seq NN 10_1101-2021_01_07_425794 270 18 data datum NNS 10_1101-2021_01_07_425794 270 19 generated generate VBN 10_1101-2021_01_07_425794 270 20 by by IN 10_1101-2021_01_07_425794 270 21 the the DT 10_1101-2021_01_07_425794 270 22 recently recently RB 10_1101-2021_01_07_425794 270 23 emerged emerge VBN 10_1101-2021_01_07_425794 270 24 single single JJ 10_1101-2021_01_07_425794 270 25 - - HYPH 10_1101-2021_01_07_425794 270 26 cell cell NN 10_1101-2021_01_07_425794 270 27 sequencing sequencing NN 10_1101-2021_01_07_425794 270 28 technologies technology NNS 10_1101-2021_01_07_425794 270 29 . . . 10_1101-2021_01_07_425794 271 1 20 20 CD 10_1101-2021_01_07_425794 271 2 .CC .CC : 10_1101-2021_01_07_425794 271 3 - - HYPH 10_1101-2021_01_07_425794 271 4 BY by IN 10_1101-2021_01_07_425794 271 5 - - HYPH 10_1101-2021_01_07_425794 271 6 NC NC NNP 10_1101-2021_01_07_425794 271 7 - - HYPH 10_1101-2021_01_07_425794 271 8 ND ND NNP 10_1101-2021_01_07_425794 271 9 4.0 4.0 CD 10_1101-2021_01_07_425794 271 10 International international JJ 10_1101-2021_01_07_425794 271 11 licensemade licensemade NN 10_1101-2021_01_07_425794 271 12 available available JJ 10_1101-2021_01_07_425794 271 13 under under IN 10_1101-2021_01_07_425794 271 14 a a DT 10_1101-2021_01_07_425794 271 15 ( ( -LRB- 10_1101-2021_01_07_425794 271 16 which which WDT 10_1101-2021_01_07_425794 271 17 was be VBD 10_1101-2021_01_07_425794 271 18 not not RB 10_1101-2021_01_07_425794 271 19 certified certify VBN 10_1101-2021_01_07_425794 271 20 by by IN 10_1101-2021_01_07_425794 271 21 peer peer NN 10_1101-2021_01_07_425794 271 22 review review NN 10_1101-2021_01_07_425794 271 23 ) ) -RRB- 10_1101-2021_01_07_425794 271 24 is be VBZ 10_1101-2021_01_07_425794 271 25 the the DT 10_1101-2021_01_07_425794 271 26 author author NN 10_1101-2021_01_07_425794 271 27 / / SYM 10_1101-2021_01_07_425794 271 28 funder funder NN 10_1101-2021_01_07_425794 271 29 , , , 10_1101-2021_01_07_425794 271 30 who who WP 10_1101-2021_01_07_425794 271 31 has have VBZ 10_1101-2021_01_07_425794 271 32 granted grant VBN 10_1101-2021_01_07_425794 271 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 271 34 a a DT 10_1101-2021_01_07_425794 271 35 license license NN 10_1101-2021_01_07_425794 271 36 to to TO 10_1101-2021_01_07_425794 271 37 display display VB 10_1101-2021_01_07_425794 271 38 the the DT 10_1101-2021_01_07_425794 271 39 preprint preprint NN 10_1101-2021_01_07_425794 271 40 in in IN 10_1101-2021_01_07_425794 271 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 271 42 . . . 10_1101-2021_01_07_425794 272 1 It -PRON- PRP 10_1101-2021_01_07_425794 272 2 is be VBZ 10_1101-2021_01_07_425794 272 3 The the DT 10_1101-2021_01_07_425794 272 4 copyright copyright NN 10_1101-2021_01_07_425794 272 5 holder holder NN 10_1101-2021_01_07_425794 272 6 for for IN 10_1101-2021_01_07_425794 272 7 this this DT 10_1101-2021_01_07_425794 272 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 272 9 version version NN 10_1101-2021_01_07_425794 272 10 posted post VBD 10_1101-2021_01_07_425794 272 11 January January NNP 10_1101-2021_01_07_425794 272 12 8 8 CD 10_1101-2021_01_07_425794 272 13 , , , 10_1101-2021_01_07_425794 272 14 2021 2021 CD 10_1101-2021_01_07_425794 272 15 . . . 10_1101-2021_01_07_425794 272 16 ; ; : 10_1101-2021_01_07_425794 272 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 272 18 : : : 10_1101-2021_01_07_425794 272 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 272 20 preprint preprint JJ 10_1101-2021_01_07_425794 272 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 272 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 272 23 Same Same NNP 10_1101-2021_01_07_425794 272 24 as as IN 10_1101-2021_01_07_425794 272 25 the the DT 10_1101-2021_01_07_425794 272 26 quantification quantification NN 10_1101-2021_01_07_425794 272 27 of of IN 10_1101-2021_01_07_425794 272 28 bulk bulk NNP 10_1101-2021_01_07_425794 272 29 RNA RNA NNP 10_1101-2021_01_07_425794 272 30 - - HYPH 10_1101-2021_01_07_425794 272 31 seq seq NN 10_1101-2021_01_07_425794 272 32 data datum NNS 10_1101-2021_01_07_425794 272 33 , , , 10_1101-2021_01_07_425794 272 34 an an DT 10_1101-2021_01_07_425794 272 35 accurate accurate JJ 10_1101-2021_01_07_425794 272 36 gene gene NN 10_1101-2021_01_07_425794 272 37 annotation annotation NN 10_1101-2021_01_07_425794 272 38 is be VBZ 10_1101-2021_01_07_425794 272 39 also also RB 10_1101-2021_01_07_425794 272 40 required require VBN 10_1101-2021_01_07_425794 272 41 for for IN 10_1101-2021_01_07_425794 272 42 quantifying quantify VBG 10_1101-2021_01_07_425794 272 43 single single JJ 10_1101-2021_01_07_425794 272 44 - - HYPH 10_1101-2021_01_07_425794 272 45 cell cell NN 10_1101-2021_01_07_425794 272 46 RNA RNA NNP 10_1101-2021_01_07_425794 272 47 - - HYPH 10_1101-2021_01_07_425794 272 48 seq seq NN 10_1101-2021_01_07_425794 272 49 data datum NNS 10_1101-2021_01_07_425794 272 50 . . . 10_1101-2021_01_07_425794 273 1 It -PRON- PRP 10_1101-2021_01_07_425794 273 2 is be VBZ 10_1101-2021_01_07_425794 273 3 therefore therefore RB 10_1101-2021_01_07_425794 273 4 important important JJ 10_1101-2021_01_07_425794 273 5 to to TO 10_1101-2021_01_07_425794 273 6 understand understand VB 10_1101-2021_01_07_425794 273 7 if if IN 10_1101-2021_01_07_425794 273 8 and and CC 10_1101-2021_01_07_425794 273 9 how how WRB 10_1101-2021_01_07_425794 273 10 the the DT 10_1101-2021_01_07_425794 273 11 annotation annotation NN 10_1101-2021_01_07_425794 273 12 choice choice NN 10_1101-2021_01_07_425794 273 13 impacts impact VBZ 10_1101-2021_01_07_425794 273 14 the the DT 10_1101-2021_01_07_425794 273 15 quantification quantification NN 10_1101-2021_01_07_425794 273 16 accuracy accuracy NN 10_1101-2021_01_07_425794 273 17 of of IN 10_1101-2021_01_07_425794 273 18 the the DT 10_1101-2021_01_07_425794 273 19 single single JJ 10_1101-2021_01_07_425794 273 20 - - HYPH 10_1101-2021_01_07_425794 273 21 cell cell NN 10_1101-2021_01_07_425794 273 22 RNA RNA NNP 10_1101-2021_01_07_425794 273 23 - - HYPH 10_1101-2021_01_07_425794 273 24 seq seq NN 10_1101-2021_01_07_425794 273 25 data datum NNS 10_1101-2021_01_07_425794 273 26 as as RB 10_1101-2021_01_07_425794 273 27 well well RB 10_1101-2021_01_07_425794 273 28 . . . 10_1101-2021_01_07_425794 274 1 References reference NNS 10_1101-2021_01_07_425794 274 2 [ [ -LRB- 10_1101-2021_01_07_425794 274 3 1 1 CD 10_1101-2021_01_07_425794 274 4 ] ] -RRB- 10_1101-2021_01_07_425794 274 5 Zhenqiang Zhenqiang NNP 10_1101-2021_01_07_425794 274 6 Su Su NNP 10_1101-2021_01_07_425794 274 7 , , , 10_1101-2021_01_07_425794 274 8 Pawe Pawe NNP 10_1101-2021_01_07_425794 274 9 l l NNP 10_1101-2021_01_07_425794 274 10 P P NNP 10_1101-2021_01_07_425794 274 11 Labaj Labaj NNP 10_1101-2021_01_07_425794 274 12 , , , 10_1101-2021_01_07_425794 274 13 Sheng Sheng NNP 10_1101-2021_01_07_425794 274 14 Li Li NNP 10_1101-2021_01_07_425794 274 15 , , , 10_1101-2021_01_07_425794 274 16 Jean Jean NNP 10_1101-2021_01_07_425794 274 17 Thierry Thierry NNP 10_1101-2021_01_07_425794 274 18 - - HYPH 10_1101-2021_01_07_425794 274 19 Mieg Mieg NNP 10_1101-2021_01_07_425794 274 20 , , , 10_1101-2021_01_07_425794 274 21 Danielle Danielle NNP 10_1101-2021_01_07_425794 274 22 Thierry Thierry NNP 10_1101-2021_01_07_425794 274 23 - - HYPH 10_1101-2021_01_07_425794 274 24 Mieg Mieg NNP 10_1101-2021_01_07_425794 274 25 , , , 10_1101-2021_01_07_425794 274 26 Wei Wei NNP 10_1101-2021_01_07_425794 274 27 Shi Shi NNP 10_1101-2021_01_07_425794 274 28 , , , 10_1101-2021_01_07_425794 274 29 Charles Charles NNP 10_1101-2021_01_07_425794 274 30 Wang Wang NNP 10_1101-2021_01_07_425794 274 31 , , , 10_1101-2021_01_07_425794 274 32 Gary Gary NNP 10_1101-2021_01_07_425794 274 33 P P NNP 10_1101-2021_01_07_425794 274 34 Schroth Schroth NNP 10_1101-2021_01_07_425794 274 35 , , , 10_1101-2021_01_07_425794 274 36 Robert Robert NNP 10_1101-2021_01_07_425794 274 37 A A NNP 10_1101-2021_01_07_425794 274 38 Setterquist Setterquist NNP 10_1101-2021_01_07_425794 274 39 , , , 10_1101-2021_01_07_425794 274 40 John John NNP 10_1101-2021_01_07_425794 274 41 F F NNP 10_1101-2021_01_07_425794 274 42 Thomp- Thomp- NNP 10_1101-2021_01_07_425794 274 43 son son NN 10_1101-2021_01_07_425794 274 44 , , , 10_1101-2021_01_07_425794 274 45 et et NNP 10_1101-2021_01_07_425794 274 46 al al NNP 10_1101-2021_01_07_425794 274 47 . . . 10_1101-2021_01_07_425794 275 1 A a DT 10_1101-2021_01_07_425794 275 2 comprehensive comprehensive JJ 10_1101-2021_01_07_425794 275 3 assessment assessment NN 10_1101-2021_01_07_425794 275 4 of of IN 10_1101-2021_01_07_425794 275 5 RNA RNA NNP 10_1101-2021_01_07_425794 275 6 - - HYPH 10_1101-2021_01_07_425794 275 7 seq seq NN 10_1101-2021_01_07_425794 275 8 accuracy accuracy NN 10_1101-2021_01_07_425794 275 9 , , , 10_1101-2021_01_07_425794 275 10 reproducibility reproducibility NN 10_1101-2021_01_07_425794 275 11 and and CC 10_1101-2021_01_07_425794 275 12 information information NN 10_1101-2021_01_07_425794 275 13 content content NN 10_1101-2021_01_07_425794 275 14 by by IN 10_1101-2021_01_07_425794 275 15 the the DT 10_1101-2021_01_07_425794 275 16 Sequencing Sequencing NNP 10_1101-2021_01_07_425794 275 17 Quality Quality NNP 10_1101-2021_01_07_425794 275 18 Control Control NNP 10_1101-2021_01_07_425794 275 19 Consortium Consortium NNP 10_1101-2021_01_07_425794 275 20 . . . 10_1101-2021_01_07_425794 276 1 Nature Nature NNP 10_1101-2021_01_07_425794 276 2 Biotech- Biotech- NNP 10_1101-2021_01_07_425794 276 3 nology nology NN 10_1101-2021_01_07_425794 276 4 , , , 10_1101-2021_01_07_425794 276 5 32(9):903 32(9):903 CD 10_1101-2021_01_07_425794 276 6 , , , 10_1101-2021_01_07_425794 276 7 2014 2014 CD 10_1101-2021_01_07_425794 276 8 . . . 10_1101-2021_01_07_425794 277 1 [ [ -LRB- 10_1101-2021_01_07_425794 277 2 2 2 LS 10_1101-2021_01_07_425794 277 3 ] ] -RRB- 10_1101-2021_01_07_425794 277 4 Yunshun Yunshun NNP 10_1101-2021_01_07_425794 277 5 Chen Chen NNP 10_1101-2021_01_07_425794 277 6 , , , 10_1101-2021_01_07_425794 277 7 Aaron Aaron NNP 10_1101-2021_01_07_425794 277 8 TL TL NNP 10_1101-2021_01_07_425794 277 9 Lun Lun NNP 10_1101-2021_01_07_425794 277 10 , , , 10_1101-2021_01_07_425794 277 11 and and CC 10_1101-2021_01_07_425794 277 12 Gordon Gordon NNP 10_1101-2021_01_07_425794 277 13 K K NNP 10_1101-2021_01_07_425794 277 14 Smyth Smyth NNP 10_1101-2021_01_07_425794 277 15 . . . 10_1101-2021_01_07_425794 278 1 From from IN 10_1101-2021_01_07_425794 278 2 reads read NNS 10_1101-2021_01_07_425794 278 3 to to IN 10_1101-2021_01_07_425794 278 4 genes gene NNS 10_1101-2021_01_07_425794 278 5 to to IN 10_1101-2021_01_07_425794 278 6 pathways pathway NNS 10_1101-2021_01_07_425794 278 7 : : : 10_1101-2021_01_07_425794 278 8 differential differential VB 10_1101-2021_01_07_425794 278 9 expression expression NN 10_1101-2021_01_07_425794 278 10 analysis analysis NN 10_1101-2021_01_07_425794 278 11 of of IN 10_1101-2021_01_07_425794 278 12 RNA RNA NNP 10_1101-2021_01_07_425794 278 13 - - HYPH 10_1101-2021_01_07_425794 278 14 Seq Seq NNP 10_1101-2021_01_07_425794 278 15 experiments experiment NNS 10_1101-2021_01_07_425794 278 16 using use VBG 10_1101-2021_01_07_425794 278 17 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 278 18 and and CC 10_1101-2021_01_07_425794 278 19 the the DT 10_1101-2021_01_07_425794 278 20 edgeR edgeR NNP 10_1101-2021_01_07_425794 278 21 quasi quasi NNP 10_1101-2021_01_07_425794 278 22 - - HYPH 10_1101-2021_01_07_425794 278 23 likelihood likelihood NNP 10_1101-2021_01_07_425794 278 24 pipeline pipeline NN 10_1101-2021_01_07_425794 278 25 . . . 10_1101-2021_01_07_425794 279 1 F1000Research F1000Research NNP 10_1101-2021_01_07_425794 279 2 , , , 10_1101-2021_01_07_425794 279 3 5:1438 5:1438 CD 10_1101-2021_01_07_425794 279 4 , , , 10_1101-2021_01_07_425794 279 5 2016 2016 CD 10_1101-2021_01_07_425794 279 6 . . . 10_1101-2021_01_07_425794 280 1 [ [ -LRB- 10_1101-2021_01_07_425794 280 2 3 3 LS 10_1101-2021_01_07_425794 280 3 ] ] -RRB- 10_1101-2021_01_07_425794 280 4 Simon Simon NNP 10_1101-2021_01_07_425794 280 5 Anders Anders NNP 10_1101-2021_01_07_425794 280 6 , , , 10_1101-2021_01_07_425794 280 7 Paul Paul NNP 10_1101-2021_01_07_425794 280 8 T T NNP 10_1101-2021_01_07_425794 280 9 Pyl Pyl NNP 10_1101-2021_01_07_425794 280 10 , , , 10_1101-2021_01_07_425794 280 11 and and CC 10_1101-2021_01_07_425794 280 12 Wolfgang Wolfgang NNP 10_1101-2021_01_07_425794 280 13 Huber Huber NNP 10_1101-2021_01_07_425794 280 14 . . . 10_1101-2021_01_07_425794 281 1 HTSeq HTSeq NNP 10_1101-2021_01_07_425794 281 2 – – : 10_1101-2021_01_07_425794 281 3 a a DT 10_1101-2021_01_07_425794 281 4 Python Python NNP 10_1101-2021_01_07_425794 281 5 framework framework NN 10_1101-2021_01_07_425794 281 6 to to TO 10_1101-2021_01_07_425794 281 7 work work VB 10_1101-2021_01_07_425794 281 8 with with IN 10_1101-2021_01_07_425794 281 9 high high JJ 10_1101-2021_01_07_425794 281 10 - - HYPH 10_1101-2021_01_07_425794 281 11 throughput throughput NN 10_1101-2021_01_07_425794 281 12 sequencing sequencing NN 10_1101-2021_01_07_425794 281 13 data datum NNS 10_1101-2021_01_07_425794 281 14 . . . 10_1101-2021_01_07_425794 282 1 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425794 282 2 , , , 10_1101-2021_01_07_425794 282 3 31(2):166–9 31(2):166–9 NNP 10_1101-2021_01_07_425794 282 4 , , , 10_1101-2021_01_07_425794 282 5 2015 2015 CD 10_1101-2021_01_07_425794 282 6 . . . 10_1101-2021_01_07_425794 283 1 [ [ -LRB- 10_1101-2021_01_07_425794 283 2 4 4 CD 10_1101-2021_01_07_425794 283 3 ] ] -RRB- 10_1101-2021_01_07_425794 283 4 Yang Yang NNP 10_1101-2021_01_07_425794 283 5 Liao Liao NNP 10_1101-2021_01_07_425794 283 6 , , , 10_1101-2021_01_07_425794 283 7 Gordon Gordon NNP 10_1101-2021_01_07_425794 283 8 K K NNP 10_1101-2021_01_07_425794 283 9 Smyth Smyth NNP 10_1101-2021_01_07_425794 283 10 , , , 10_1101-2021_01_07_425794 283 11 and and CC 10_1101-2021_01_07_425794 283 12 Wei Wei NNP 10_1101-2021_01_07_425794 283 13 Shi Shi NNP 10_1101-2021_01_07_425794 283 14 . . . 10_1101-2021_01_07_425794 284 1 featureCounts featurecount NNS 10_1101-2021_01_07_425794 284 2 : : : 10_1101-2021_01_07_425794 284 3 an an DT 10_1101-2021_01_07_425794 284 4 efficient efficient JJ 10_1101-2021_01_07_425794 284 5 general general JJ 10_1101-2021_01_07_425794 284 6 purpose purpose NN 10_1101-2021_01_07_425794 284 7 program program NN 10_1101-2021_01_07_425794 284 8 for for IN 10_1101-2021_01_07_425794 284 9 assigning assigning NN 10_1101-2021_01_07_425794 284 10 sequence sequence NN 10_1101-2021_01_07_425794 284 11 reads read VBZ 10_1101-2021_01_07_425794 284 12 to to IN 10_1101-2021_01_07_425794 284 13 genomic genomic JJ 10_1101-2021_01_07_425794 284 14 features feature NNS 10_1101-2021_01_07_425794 284 15 . . . 10_1101-2021_01_07_425794 285 1 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425794 285 2 , , , 10_1101-2021_01_07_425794 285 3 30(7):923–930 30(7):923–930 CD 10_1101-2021_01_07_425794 285 4 , , , 10_1101-2021_01_07_425794 285 5 2014 2014 CD 10_1101-2021_01_07_425794 285 6 . . . 10_1101-2021_01_07_425794 286 1 [ [ -LRB- 10_1101-2021_01_07_425794 286 2 5 5 CD 10_1101-2021_01_07_425794 286 3 ] ] -RRB- 10_1101-2021_01_07_425794 286 4 Yang Yang NNP 10_1101-2021_01_07_425794 286 5 Liao Liao NNP 10_1101-2021_01_07_425794 286 6 , , , 10_1101-2021_01_07_425794 286 7 Gordon Gordon NNP 10_1101-2021_01_07_425794 286 8 K K NNP 10_1101-2021_01_07_425794 286 9 Smyth Smyth NNP 10_1101-2021_01_07_425794 286 10 , , , 10_1101-2021_01_07_425794 286 11 and and CC 10_1101-2021_01_07_425794 286 12 Wei Wei NNP 10_1101-2021_01_07_425794 286 13 Shi Shi NNP 10_1101-2021_01_07_425794 286 14 . . . 10_1101-2021_01_07_425794 287 1 The the DT 10_1101-2021_01_07_425794 287 2 R r NN 10_1101-2021_01_07_425794 287 3 package package NN 10_1101-2021_01_07_425794 287 4 Rsubread Rsubread NNP 10_1101-2021_01_07_425794 287 5 is be VBZ 10_1101-2021_01_07_425794 287 6 easier easy JJR 10_1101-2021_01_07_425794 287 7 , , , 10_1101-2021_01_07_425794 287 8 faster fast JJR 10_1101-2021_01_07_425794 287 9 , , , 10_1101-2021_01_07_425794 287 10 cheaper cheap JJR 10_1101-2021_01_07_425794 287 11 and and CC 10_1101-2021_01_07_425794 287 12 better well JJR 10_1101-2021_01_07_425794 287 13 for for IN 10_1101-2021_01_07_425794 287 14 alignment alignment NN 10_1101-2021_01_07_425794 287 15 and and CC 10_1101-2021_01_07_425794 287 16 quantification quantification NN 10_1101-2021_01_07_425794 287 17 of of IN 10_1101-2021_01_07_425794 287 18 RNA RNA NNP 10_1101-2021_01_07_425794 287 19 sequencing sequencing NN 10_1101-2021_01_07_425794 287 20 reads read VBZ 10_1101-2021_01_07_425794 287 21 . . . 10_1101-2021_01_07_425794 288 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 288 2 Acids Acids NNP 10_1101-2021_01_07_425794 288 3 Research Research NNP 10_1101-2021_01_07_425794 288 4 , , , 10_1101-2021_01_07_425794 288 5 47(8):e47–e47 47(8):e47–e47 CD 10_1101-2021_01_07_425794 288 6 , , , 10_1101-2021_01_07_425794 288 7 2019 2019 CD 10_1101-2021_01_07_425794 288 8 . . . 10_1101-2021_01_07_425794 289 1 [ [ -LRB- 10_1101-2021_01_07_425794 289 2 6 6 CD 10_1101-2021_01_07_425794 289 3 ] ] -RRB- 10_1101-2021_01_07_425794 289 4 Steven Steven NNP 10_1101-2021_01_07_425794 289 5 L. L. NNP 10_1101-2021_01_07_425794 289 6 Salzberg Salzberg NNP 10_1101-2021_01_07_425794 289 7 . . . 10_1101-2021_01_07_425794 290 1 Next next JJ 10_1101-2021_01_07_425794 290 2 - - HYPH 10_1101-2021_01_07_425794 290 3 generation generation NN 10_1101-2021_01_07_425794 290 4 genome genome JJ 10_1101-2021_01_07_425794 290 5 annotation annotation NN 10_1101-2021_01_07_425794 290 6 : : : 10_1101-2021_01_07_425794 290 7 we -PRON- PRP 10_1101-2021_01_07_425794 290 8 still still RB 10_1101-2021_01_07_425794 290 9 struggle struggle VBP 10_1101-2021_01_07_425794 290 10 to to TO 10_1101-2021_01_07_425794 290 11 get get VB 10_1101-2021_01_07_425794 290 12 it -PRON- PRP 10_1101-2021_01_07_425794 290 13 right right JJ 10_1101-2021_01_07_425794 290 14 . . . 10_1101-2021_01_07_425794 291 1 Genome Genome NNP 10_1101-2021_01_07_425794 291 2 Biology Biology NNP 10_1101-2021_01_07_425794 291 3 , , , 10_1101-2021_01_07_425794 291 4 20(1):92 20(1):92 NNP 10_1101-2021_01_07_425794 291 5 , , , 10_1101-2021_01_07_425794 291 6 2019 2019 CD 10_1101-2021_01_07_425794 291 7 . . . 10_1101-2021_01_07_425794 292 1 [ [ -LRB- 10_1101-2021_01_07_425794 292 2 7 7 CD 10_1101-2021_01_07_425794 292 3 ] ] -RRB- 10_1101-2021_01_07_425794 292 4 Mihaela Mihaela NNP 10_1101-2021_01_07_425794 292 5 Pertea Pertea NNP 10_1101-2021_01_07_425794 292 6 , , , 10_1101-2021_01_07_425794 292 7 Alaina Alaina NNP 10_1101-2021_01_07_425794 292 8 Shumate Shumate NNP 10_1101-2021_01_07_425794 292 9 , , , 10_1101-2021_01_07_425794 292 10 Geo Geo NNP 10_1101-2021_01_07_425794 292 11 Pertea Pertea NNP 10_1101-2021_01_07_425794 292 12 , , , 10_1101-2021_01_07_425794 292 13 Ales Ales NNP 10_1101-2021_01_07_425794 292 14 Varabyou Varabyou NNP 10_1101-2021_01_07_425794 292 15 , , , 10_1101-2021_01_07_425794 292 16 Florian Florian NNP 10_1101-2021_01_07_425794 292 17 P P NNP 10_1101-2021_01_07_425794 292 18 Breitwieser Breitwieser NNP 10_1101-2021_01_07_425794 292 19 , , , 10_1101-2021_01_07_425794 292 20 Yu Yu NNP 10_1101-2021_01_07_425794 292 21 - - HYPH 10_1101-2021_01_07_425794 292 22 Chi Chi NNP 10_1101-2021_01_07_425794 292 23 Chang Chang NNP 10_1101-2021_01_07_425794 292 24 , , , 10_1101-2021_01_07_425794 292 25 Anil Anil NNP 10_1101-2021_01_07_425794 292 26 K K NNP 10_1101-2021_01_07_425794 292 27 Madugundu Madugundu NNP 10_1101-2021_01_07_425794 292 28 , , , 10_1101-2021_01_07_425794 292 29 Akhilesh Akhilesh NNP 10_1101-2021_01_07_425794 292 30 Pandey Pandey NNP 10_1101-2021_01_07_425794 292 31 , , , 10_1101-2021_01_07_425794 292 32 and and CC 10_1101-2021_01_07_425794 292 33 Steven Steven NNP 10_1101-2021_01_07_425794 292 34 L L NNP 10_1101-2021_01_07_425794 292 35 Salzberg Salzberg NNP 10_1101-2021_01_07_425794 292 36 . . . 10_1101-2021_01_07_425794 293 1 CHESS CHESS NNP 10_1101-2021_01_07_425794 293 2 : : : 10_1101-2021_01_07_425794 293 3 a a DT 10_1101-2021_01_07_425794 293 4 new new JJ 10_1101-2021_01_07_425794 293 5 human human JJ 10_1101-2021_01_07_425794 293 6 gene gene NN 10_1101-2021_01_07_425794 293 7 catalog catalog NN 10_1101-2021_01_07_425794 293 8 curated curate VBN 10_1101-2021_01_07_425794 293 9 from from IN 10_1101-2021_01_07_425794 293 10 thousands thousand NNS 10_1101-2021_01_07_425794 293 11 of of IN 10_1101-2021_01_07_425794 293 12 large large JJ 10_1101-2021_01_07_425794 293 13 - - HYPH 10_1101-2021_01_07_425794 293 14 scale scale NN 10_1101-2021_01_07_425794 293 15 RNA rna NN 10_1101-2021_01_07_425794 293 16 sequencing sequence VBG 10_1101-2021_01_07_425794 293 17 experiments experiment NNS 10_1101-2021_01_07_425794 293 18 reveals reveal VBZ 10_1101-2021_01_07_425794 293 19 extensive extensive JJ 10_1101-2021_01_07_425794 293 20 transcriptional transcriptional JJ 10_1101-2021_01_07_425794 293 21 noise noise NN 10_1101-2021_01_07_425794 293 22 . . . 10_1101-2021_01_07_425794 294 1 Genome Genome NNP 10_1101-2021_01_07_425794 294 2 Biology Biology NNP 10_1101-2021_01_07_425794 294 3 , , , 10_1101-2021_01_07_425794 294 4 19(1):208 19(1):208 CD 10_1101-2021_01_07_425794 294 5 , , , 10_1101-2021_01_07_425794 294 6 2018 2018 CD 10_1101-2021_01_07_425794 294 7 . . . 10_1101-2021_01_07_425794 295 1 [ [ -LRB- 10_1101-2021_01_07_425794 295 2 8 8 CD 10_1101-2021_01_07_425794 295 3 ] ] -RRB- 10_1101-2021_01_07_425794 295 4 Daniel Daniel NNP 10_1101-2021_01_07_425794 295 5 R R NNP 10_1101-2021_01_07_425794 295 6 Zerbino Zerbino NNP 10_1101-2021_01_07_425794 295 7 , , , 10_1101-2021_01_07_425794 295 8 Premanand Premanand NNP 10_1101-2021_01_07_425794 295 9 Achuthan Achuthan NNP 10_1101-2021_01_07_425794 295 10 , , , 10_1101-2021_01_07_425794 295 11 Wasiu Wasiu NNP 10_1101-2021_01_07_425794 295 12 Akanni Akanni NNP 10_1101-2021_01_07_425794 295 13 , , , 10_1101-2021_01_07_425794 295 14 M M NNP 10_1101-2021_01_07_425794 295 15 Ridwan Ridwan NNP 10_1101-2021_01_07_425794 295 16 Amode Amode NNP 10_1101-2021_01_07_425794 295 17 , , , 10_1101-2021_01_07_425794 295 18 Daniel Daniel NNP 10_1101-2021_01_07_425794 295 19 Barrell Barrell NNP 10_1101-2021_01_07_425794 295 20 , , , 10_1101-2021_01_07_425794 295 21 Jyothish Jyothish NNP 10_1101-2021_01_07_425794 295 22 Bhai Bhai NNP 10_1101-2021_01_07_425794 295 23 , , , 10_1101-2021_01_07_425794 295 24 Konstantinos Konstantinos NNP 10_1101-2021_01_07_425794 295 25 Billis Billis NNP 10_1101-2021_01_07_425794 295 26 , , , 10_1101-2021_01_07_425794 295 27 Carla Carla NNP 10_1101-2021_01_07_425794 295 28 Cummins Cummins NNP 10_1101-2021_01_07_425794 295 29 , , , 10_1101-2021_01_07_425794 295 30 Astrid Astrid NNP 10_1101-2021_01_07_425794 295 31 Gall Gall NNP 10_1101-2021_01_07_425794 295 32 , , , 10_1101-2021_01_07_425794 295 33 Car- Car- NNP 10_1101-2021_01_07_425794 295 34 los los NNP 10_1101-2021_01_07_425794 295 35 Garćıa Garćıa NNP 10_1101-2021_01_07_425794 295 36 Girón Girón NNP 10_1101-2021_01_07_425794 295 37 , , , 10_1101-2021_01_07_425794 295 38 et et NNP 10_1101-2021_01_07_425794 295 39 al al NNP 10_1101-2021_01_07_425794 295 40 . . . 10_1101-2021_01_07_425794 296 1 Ensembl Ensembl NNP 10_1101-2021_01_07_425794 296 2 2018 2018 CD 10_1101-2021_01_07_425794 296 3 . . . 10_1101-2021_01_07_425794 297 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 297 2 Acids Acids NNP 10_1101-2021_01_07_425794 297 3 Research Research NNP 10_1101-2021_01_07_425794 297 4 , , , 10_1101-2021_01_07_425794 297 5 46(D1):D754–D761 46(D1):D754–D761 NNP 10_1101-2021_01_07_425794 297 6 , , , 10_1101-2021_01_07_425794 297 7 2018 2018 CD 10_1101-2021_01_07_425794 297 8 . . . 10_1101-2021_01_07_425794 298 1 21 21 CD 10_1101-2021_01_07_425794 298 2 .CC .CC : 10_1101-2021_01_07_425794 298 3 - - HYPH 10_1101-2021_01_07_425794 298 4 BY by IN 10_1101-2021_01_07_425794 298 5 - - HYPH 10_1101-2021_01_07_425794 298 6 NC NC NNP 10_1101-2021_01_07_425794 298 7 - - HYPH 10_1101-2021_01_07_425794 298 8 ND ND NNP 10_1101-2021_01_07_425794 298 9 4.0 4.0 CD 10_1101-2021_01_07_425794 298 10 International international JJ 10_1101-2021_01_07_425794 298 11 licensemade licensemade NN 10_1101-2021_01_07_425794 298 12 available available JJ 10_1101-2021_01_07_425794 298 13 under under IN 10_1101-2021_01_07_425794 298 14 a a DT 10_1101-2021_01_07_425794 298 15 ( ( -LRB- 10_1101-2021_01_07_425794 298 16 which which WDT 10_1101-2021_01_07_425794 298 17 was be VBD 10_1101-2021_01_07_425794 298 18 not not RB 10_1101-2021_01_07_425794 298 19 certified certify VBN 10_1101-2021_01_07_425794 298 20 by by IN 10_1101-2021_01_07_425794 298 21 peer peer NN 10_1101-2021_01_07_425794 298 22 review review NN 10_1101-2021_01_07_425794 298 23 ) ) -RRB- 10_1101-2021_01_07_425794 298 24 is be VBZ 10_1101-2021_01_07_425794 298 25 the the DT 10_1101-2021_01_07_425794 298 26 author author NN 10_1101-2021_01_07_425794 298 27 / / SYM 10_1101-2021_01_07_425794 298 28 funder funder NN 10_1101-2021_01_07_425794 298 29 , , , 10_1101-2021_01_07_425794 298 30 who who WP 10_1101-2021_01_07_425794 298 31 has have VBZ 10_1101-2021_01_07_425794 298 32 granted grant VBN 10_1101-2021_01_07_425794 298 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 298 34 a a DT 10_1101-2021_01_07_425794 298 35 license license NN 10_1101-2021_01_07_425794 298 36 to to TO 10_1101-2021_01_07_425794 298 37 display display VB 10_1101-2021_01_07_425794 298 38 the the DT 10_1101-2021_01_07_425794 298 39 preprint preprint NN 10_1101-2021_01_07_425794 298 40 in in IN 10_1101-2021_01_07_425794 298 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 298 42 . . . 10_1101-2021_01_07_425794 299 1 It -PRON- PRP 10_1101-2021_01_07_425794 299 2 is be VBZ 10_1101-2021_01_07_425794 299 3 The the DT 10_1101-2021_01_07_425794 299 4 copyright copyright NN 10_1101-2021_01_07_425794 299 5 holder holder NN 10_1101-2021_01_07_425794 299 6 for for IN 10_1101-2021_01_07_425794 299 7 this this DT 10_1101-2021_01_07_425794 299 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 299 9 version version NN 10_1101-2021_01_07_425794 299 10 posted post VBD 10_1101-2021_01_07_425794 299 11 January January NNP 10_1101-2021_01_07_425794 299 12 8 8 CD 10_1101-2021_01_07_425794 299 13 , , , 10_1101-2021_01_07_425794 299 14 2021 2021 CD 10_1101-2021_01_07_425794 299 15 . . . 10_1101-2021_01_07_425794 299 16 ; ; : 10_1101-2021_01_07_425794 299 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 299 18 : : : 10_1101-2021_01_07_425794 299 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 299 20 preprint preprint JJ 10_1101-2021_01_07_425794 299 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 299 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 299 23 [ [ -LRB- 10_1101-2021_01_07_425794 299 24 9 9 CD 10_1101-2021_01_07_425794 299 25 ] ] -RRB- 10_1101-2021_01_07_425794 299 26 Nuala Nuala NNP 10_1101-2021_01_07_425794 299 27 A A NNP 10_1101-2021_01_07_425794 299 28 O’Leary O’Leary NNP 10_1101-2021_01_07_425794 299 29 , , , 10_1101-2021_01_07_425794 299 30 Mathew Mathew NNP 10_1101-2021_01_07_425794 299 31 W W NNP 10_1101-2021_01_07_425794 299 32 Wright Wright NNP 10_1101-2021_01_07_425794 299 33 , , , 10_1101-2021_01_07_425794 299 34 J J NNP 10_1101-2021_01_07_425794 299 35 Rodney Rodney NNP 10_1101-2021_01_07_425794 299 36 Brister Brister NNP 10_1101-2021_01_07_425794 299 37 , , , 10_1101-2021_01_07_425794 299 38 Stacy Stacy NNP 10_1101-2021_01_07_425794 299 39 Ciufo Ciufo NNP 10_1101-2021_01_07_425794 299 40 , , , 10_1101-2021_01_07_425794 299 41 Diana Diana NNP 10_1101-2021_01_07_425794 299 42 Haddad Haddad NNP 10_1101-2021_01_07_425794 299 43 , , , 10_1101-2021_01_07_425794 299 44 Rich Rich NNP 10_1101-2021_01_07_425794 299 45 McVeigh McVeigh NNP 10_1101-2021_01_07_425794 299 46 , , , 10_1101-2021_01_07_425794 299 47 Bhanu Bhanu NNP 10_1101-2021_01_07_425794 299 48 Rajput Rajput NNP 10_1101-2021_01_07_425794 299 49 , , , 10_1101-2021_01_07_425794 299 50 Barbara Barbara NNP 10_1101-2021_01_07_425794 299 51 Robbertse Robbertse NNP 10_1101-2021_01_07_425794 299 52 , , , 10_1101-2021_01_07_425794 299 53 Brian Brian NNP 10_1101-2021_01_07_425794 299 54 Smith Smith NNP 10_1101-2021_01_07_425794 299 55 - - HYPH 10_1101-2021_01_07_425794 299 56 White White NNP 10_1101-2021_01_07_425794 299 57 , , , 10_1101-2021_01_07_425794 299 58 Danso Danso NNP 10_1101-2021_01_07_425794 299 59 Ako- Ako- NNP 10_1101-2021_01_07_425794 299 60 Adjei adjei UH 10_1101-2021_01_07_425794 299 61 , , , 10_1101-2021_01_07_425794 299 62 et et NNP 10_1101-2021_01_07_425794 299 63 al al NNP 10_1101-2021_01_07_425794 299 64 . . . 10_1101-2021_01_07_425794 300 1 Reference reference NN 10_1101-2021_01_07_425794 300 2 sequence sequence NN 10_1101-2021_01_07_425794 300 3 ( ( -LRB- 10_1101-2021_01_07_425794 300 4 RefSeq RefSeq NNP 10_1101-2021_01_07_425794 300 5 ) ) -RRB- 10_1101-2021_01_07_425794 300 6 database database NN 10_1101-2021_01_07_425794 300 7 at at IN 10_1101-2021_01_07_425794 300 8 NCBI NCBI NNP 10_1101-2021_01_07_425794 300 9 : : : 10_1101-2021_01_07_425794 300 10 current current JJ 10_1101-2021_01_07_425794 300 11 status status NN 10_1101-2021_01_07_425794 300 12 , , , 10_1101-2021_01_07_425794 300 13 taxo- taxo- JJ 10_1101-2021_01_07_425794 300 14 nomic nomic JJ 10_1101-2021_01_07_425794 300 15 expansion expansion NN 10_1101-2021_01_07_425794 300 16 , , , 10_1101-2021_01_07_425794 300 17 and and CC 10_1101-2021_01_07_425794 300 18 functional functional JJ 10_1101-2021_01_07_425794 300 19 annotation annotation NN 10_1101-2021_01_07_425794 300 20 . . . 10_1101-2021_01_07_425794 301 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 301 2 Acids Acids NNPS 10_1101-2021_01_07_425794 301 3 Research Research NNP 10_1101-2021_01_07_425794 301 4 , , , 10_1101-2021_01_07_425794 301 5 44(D1):D733 44(d1):d733 CD 10_1101-2021_01_07_425794 301 6 – – : 10_1101-2021_01_07_425794 301 7 D745 d745 NN 10_1101-2021_01_07_425794 301 8 , , , 10_1101-2021_01_07_425794 301 9 2016 2016 CD 10_1101-2021_01_07_425794 301 10 . . . 10_1101-2021_01_07_425794 302 1 [ [ -LRB- 10_1101-2021_01_07_425794 302 2 10 10 CD 10_1101-2021_01_07_425794 302 3 ] ] -RRB- 10_1101-2021_01_07_425794 302 4 Adam Adam NNP 10_1101-2021_01_07_425794 302 5 Frankish Frankish NNP 10_1101-2021_01_07_425794 302 6 , , , 10_1101-2021_01_07_425794 302 7 Mark Mark NNP 10_1101-2021_01_07_425794 302 8 Diekhans Diekhans NNPS 10_1101-2021_01_07_425794 302 9 , , , 10_1101-2021_01_07_425794 302 10 Anne Anne NNP 10_1101-2021_01_07_425794 302 11 - - HYPH 10_1101-2021_01_07_425794 302 12 Maud Maud NNP 10_1101-2021_01_07_425794 302 13 Ferreira Ferreira NNP 10_1101-2021_01_07_425794 302 14 , , , 10_1101-2021_01_07_425794 302 15 Rory Rory NNP 10_1101-2021_01_07_425794 302 16 Johnson Johnson NNP 10_1101-2021_01_07_425794 302 17 , , , 10_1101-2021_01_07_425794 302 18 Irwin Irwin NNP 10_1101-2021_01_07_425794 302 19 Jun- Jun- NNP 10_1101-2021_01_07_425794 302 20 greis greis NN 10_1101-2021_01_07_425794 302 21 , , , 10_1101-2021_01_07_425794 302 22 Jane Jane NNP 10_1101-2021_01_07_425794 302 23 Loveland Loveland NNP 10_1101-2021_01_07_425794 302 24 , , , 10_1101-2021_01_07_425794 302 25 Jonathan Jonathan NNP 10_1101-2021_01_07_425794 302 26 M M NNP 10_1101-2021_01_07_425794 302 27 Mudge Mudge NNP 10_1101-2021_01_07_425794 302 28 , , , 10_1101-2021_01_07_425794 302 29 Cristina Cristina NNP 10_1101-2021_01_07_425794 302 30 Sisu Sisu NNP 10_1101-2021_01_07_425794 302 31 , , , 10_1101-2021_01_07_425794 302 32 James James NNP 10_1101-2021_01_07_425794 302 33 Wright Wright NNP 10_1101-2021_01_07_425794 302 34 , , , 10_1101-2021_01_07_425794 302 35 Joel Joel NNP 10_1101-2021_01_07_425794 302 36 Arm- Arm- NNP 10_1101-2021_01_07_425794 302 37 strong strong RB 10_1101-2021_01_07_425794 302 38 , , , 10_1101-2021_01_07_425794 302 39 et et NNP 10_1101-2021_01_07_425794 302 40 al al NNP 10_1101-2021_01_07_425794 302 41 . . . 10_1101-2021_01_07_425794 303 1 GENCODE gencode JJ 10_1101-2021_01_07_425794 303 2 reference reference NN 10_1101-2021_01_07_425794 303 3 annotation annotation NN 10_1101-2021_01_07_425794 303 4 for for IN 10_1101-2021_01_07_425794 303 5 the the DT 10_1101-2021_01_07_425794 303 6 human human JJ 10_1101-2021_01_07_425794 303 7 and and CC 10_1101-2021_01_07_425794 303 8 mouse mouse NN 10_1101-2021_01_07_425794 303 9 genomes genome NNS 10_1101-2021_01_07_425794 303 10 . . . 10_1101-2021_01_07_425794 304 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 304 2 Acids Acids NNPS 10_1101-2021_01_07_425794 304 3 Research Research NNP 10_1101-2021_01_07_425794 304 4 , , , 10_1101-2021_01_07_425794 304 5 47(D1):D766–D773 47(d1):d766–d773 CD 10_1101-2021_01_07_425794 304 6 , , , 10_1101-2021_01_07_425794 304 7 2019 2019 CD 10_1101-2021_01_07_425794 304 8 . . . 10_1101-2021_01_07_425794 305 1 [ [ -LRB- 10_1101-2021_01_07_425794 305 2 11 11 CD 10_1101-2021_01_07_425794 305 3 ] ] -RRB- 10_1101-2021_01_07_425794 305 4 Christopher Christopher NNP 10_1101-2021_01_07_425794 305 5 M M NNP 10_1101-2021_01_07_425794 305 6 Lee Lee NNP 10_1101-2021_01_07_425794 305 7 , , , 10_1101-2021_01_07_425794 305 8 Galt Galt NNP 10_1101-2021_01_07_425794 305 9 P P NNP 10_1101-2021_01_07_425794 305 10 Barber Barber NNP 10_1101-2021_01_07_425794 305 11 , , , 10_1101-2021_01_07_425794 305 12 Jonathan Jonathan NNP 10_1101-2021_01_07_425794 305 13 Casper Casper NNP 10_1101-2021_01_07_425794 305 14 , , , 10_1101-2021_01_07_425794 305 15 Hiram Hiram NNP 10_1101-2021_01_07_425794 305 16 Clawson Clawson NNP 10_1101-2021_01_07_425794 305 17 , , , 10_1101-2021_01_07_425794 305 18 Mark Mark NNP 10_1101-2021_01_07_425794 305 19 Diekhans Diekhans NNPS 10_1101-2021_01_07_425794 305 20 , , , 10_1101-2021_01_07_425794 305 21 Jairo Jairo NNP 10_1101-2021_01_07_425794 305 22 N N NNP 10_1101-2021_01_07_425794 305 23 Gonzalez Gonzalez NNP 10_1101-2021_01_07_425794 305 24 , , , 10_1101-2021_01_07_425794 305 25 Angie Angie NNP 10_1101-2021_01_07_425794 305 26 S S NNP 10_1101-2021_01_07_425794 305 27 Hinrichs Hinrichs NNP 10_1101-2021_01_07_425794 305 28 , , , 10_1101-2021_01_07_425794 305 29 Brian Brian NNP 10_1101-2021_01_07_425794 305 30 T T NNP 10_1101-2021_01_07_425794 305 31 Lee Lee NNP 10_1101-2021_01_07_425794 305 32 , , , 10_1101-2021_01_07_425794 305 33 Luis Luis NNP 10_1101-2021_01_07_425794 305 34 R R NNP 10_1101-2021_01_07_425794 305 35 Nassar Nassar NNP 10_1101-2021_01_07_425794 305 36 , , , 10_1101-2021_01_07_425794 305 37 Con- Con- NNP 10_1101-2021_01_07_425794 305 38 ner ner NN 10_1101-2021_01_07_425794 305 39 C C NNP 10_1101-2021_01_07_425794 305 40 Powell Powell NNP 10_1101-2021_01_07_425794 305 41 , , , 10_1101-2021_01_07_425794 305 42 Brian Brian NNP 10_1101-2021_01_07_425794 305 43 J J NNP 10_1101-2021_01_07_425794 305 44 Raney Raney NNP 10_1101-2021_01_07_425794 305 45 , , , 10_1101-2021_01_07_425794 305 46 Kate Kate NNP 10_1101-2021_01_07_425794 305 47 R R NNP 10_1101-2021_01_07_425794 305 48 Rosenbloom Rosenbloom NNP 10_1101-2021_01_07_425794 305 49 , , , 10_1101-2021_01_07_425794 305 50 Daniel Daniel NNP 10_1101-2021_01_07_425794 305 51 Schmelter Schmelter NNP 10_1101-2021_01_07_425794 305 52 , , , 10_1101-2021_01_07_425794 305 53 Matthew Matthew NNP 10_1101-2021_01_07_425794 305 54 L L NNP 10_1101-2021_01_07_425794 305 55 Speir Speir NNP 10_1101-2021_01_07_425794 305 56 , , , 10_1101-2021_01_07_425794 305 57 Ann Ann NNP 10_1101-2021_01_07_425794 305 58 S S NNP 10_1101-2021_01_07_425794 305 59 Zweig Zweig NNP 10_1101-2021_01_07_425794 305 60 , , , 10_1101-2021_01_07_425794 305 61 David David NNP 10_1101-2021_01_07_425794 305 62 Haussler Haussler NNP 10_1101-2021_01_07_425794 305 63 , , , 10_1101-2021_01_07_425794 305 64 Maximilian Maximilian NNP 10_1101-2021_01_07_425794 305 65 Haeussler Haeussler NNP 10_1101-2021_01_07_425794 305 66 , , , 10_1101-2021_01_07_425794 305 67 Robert Robert NNP 10_1101-2021_01_07_425794 305 68 M M NNP 10_1101-2021_01_07_425794 305 69 Kuhn Kuhn NNP 10_1101-2021_01_07_425794 305 70 , , , 10_1101-2021_01_07_425794 305 71 and and CC 10_1101-2021_01_07_425794 305 72 W W NNP 10_1101-2021_01_07_425794 305 73 J J NNP 10_1101-2021_01_07_425794 305 74 Kent Kent NNP 10_1101-2021_01_07_425794 305 75 . . . 10_1101-2021_01_07_425794 306 1 UCSC UCSC NNS 10_1101-2021_01_07_425794 306 2 Genome Genome NNP 10_1101-2021_01_07_425794 306 3 Browser Browser NNP 10_1101-2021_01_07_425794 306 4 enters enter VBZ 10_1101-2021_01_07_425794 306 5 20th 20th JJ 10_1101-2021_01_07_425794 306 6 year year NN 10_1101-2021_01_07_425794 306 7 . . . 10_1101-2021_01_07_425794 307 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 307 2 Acids Acids NNP 10_1101-2021_01_07_425794 307 3 Research Research NNP 10_1101-2021_01_07_425794 307 4 , , , 10_1101-2021_01_07_425794 307 5 48(D1):D756–D761 48(d1):d756–d761 CD 10_1101-2021_01_07_425794 307 6 , , , 10_1101-2021_01_07_425794 307 7 2020 2020 CD 10_1101-2021_01_07_425794 307 8 . . . 10_1101-2021_01_07_425794 308 1 [ [ -LRB- 10_1101-2021_01_07_425794 308 2 12 12 CD 10_1101-2021_01_07_425794 308 3 ] ] -RRB- 10_1101-2021_01_07_425794 308 4 Po Po NNP 10_1101-2021_01_07_425794 308 5 - - HYPH 10_1101-2021_01_07_425794 308 6 Yen Yen NNP 10_1101-2021_01_07_425794 308 7 Wu Wu NNP 10_1101-2021_01_07_425794 308 8 , , , 10_1101-2021_01_07_425794 308 9 John John NNP 10_1101-2021_01_07_425794 308 10 H H NNP 10_1101-2021_01_07_425794 308 11 Phan Phan NNP 10_1101-2021_01_07_425794 308 12 , , , 10_1101-2021_01_07_425794 308 13 and and CC 10_1101-2021_01_07_425794 308 14 May May NNP 10_1101-2021_01_07_425794 308 15 D D NNP 10_1101-2021_01_07_425794 308 16 Wang Wang NNP 10_1101-2021_01_07_425794 308 17 . . . 10_1101-2021_01_07_425794 309 1 Assessing assess VBG 10_1101-2021_01_07_425794 309 2 the the DT 10_1101-2021_01_07_425794 309 3 impact impact NN 10_1101-2021_01_07_425794 309 4 of of IN 10_1101-2021_01_07_425794 309 5 human human JJ 10_1101-2021_01_07_425794 309 6 genome genome JJ 10_1101-2021_01_07_425794 309 7 annotation annotation NN 10_1101-2021_01_07_425794 309 8 choice choice NN 10_1101-2021_01_07_425794 309 9 on on IN 10_1101-2021_01_07_425794 309 10 RNA RNA NNP 10_1101-2021_01_07_425794 309 11 - - HYPH 10_1101-2021_01_07_425794 309 12 seq seq NN 10_1101-2021_01_07_425794 309 13 expression expression NN 10_1101-2021_01_07_425794 309 14 estimates estimate NNS 10_1101-2021_01_07_425794 309 15 . . . 10_1101-2021_01_07_425794 310 1 BMC BMC NNP 10_1101-2021_01_07_425794 310 2 Bioinformatics Bioinformatics NNP 10_1101-2021_01_07_425794 310 3 , , , 10_1101-2021_01_07_425794 310 4 14(11):S8 14(11):s8 CD 10_1101-2021_01_07_425794 310 5 , , , 10_1101-2021_01_07_425794 310 6 2013 2013 CD 10_1101-2021_01_07_425794 310 7 . . . 10_1101-2021_01_07_425794 311 1 [ [ -LRB- 10_1101-2021_01_07_425794 311 2 13 13 CD 10_1101-2021_01_07_425794 311 3 ] ] -RRB- 10_1101-2021_01_07_425794 311 4 Shanrong Shanrong NNP 10_1101-2021_01_07_425794 311 5 Zhao Zhao NNP 10_1101-2021_01_07_425794 311 6 and and CC 10_1101-2021_01_07_425794 311 7 Baohong Baohong NNP 10_1101-2021_01_07_425794 311 8 Zhang Zhang NNP 10_1101-2021_01_07_425794 311 9 . . . 10_1101-2021_01_07_425794 312 1 A a DT 10_1101-2021_01_07_425794 312 2 comprehensive comprehensive JJ 10_1101-2021_01_07_425794 312 3 evaluation evaluation NN 10_1101-2021_01_07_425794 312 4 of of IN 10_1101-2021_01_07_425794 312 5 ensembl ensembl NNP 10_1101-2021_01_07_425794 312 6 , , , 10_1101-2021_01_07_425794 312 7 Ref- Ref- NNP 10_1101-2021_01_07_425794 312 8 Seq Seq NNP 10_1101-2021_01_07_425794 312 9 , , , 10_1101-2021_01_07_425794 312 10 and and CC 10_1101-2021_01_07_425794 312 11 UCSC UCSC NNP 10_1101-2021_01_07_425794 312 12 annotations annotation NNS 10_1101-2021_01_07_425794 312 13 in in IN 10_1101-2021_01_07_425794 312 14 the the DT 10_1101-2021_01_07_425794 312 15 context context NN 10_1101-2021_01_07_425794 312 16 of of IN 10_1101-2021_01_07_425794 312 17 RNA RNA NNP 10_1101-2021_01_07_425794 312 18 - - HYPH 10_1101-2021_01_07_425794 312 19 seq seq NN 10_1101-2021_01_07_425794 312 20 read read VBP 10_1101-2021_01_07_425794 312 21 mapping mapping NN 10_1101-2021_01_07_425794 312 22 and and CC 10_1101-2021_01_07_425794 312 23 gene gene NN 10_1101-2021_01_07_425794 312 24 quantification quantification NN 10_1101-2021_01_07_425794 312 25 . . . 10_1101-2021_01_07_425794 313 1 BMC BMC NNP 10_1101-2021_01_07_425794 313 2 Genomics Genomics NNP 10_1101-2021_01_07_425794 313 3 , , , 10_1101-2021_01_07_425794 313 4 16(1):97 16(1):97 NNP 10_1101-2021_01_07_425794 313 5 , , , 10_1101-2021_01_07_425794 313 6 2015 2015 CD 10_1101-2021_01_07_425794 313 7 . . . 10_1101-2021_01_07_425794 314 1 [ [ -LRB- 10_1101-2021_01_07_425794 314 2 14 14 CD 10_1101-2021_01_07_425794 314 3 ] ] -RRB- 10_1101-2021_01_07_425794 314 4 Leming Leming NNP 10_1101-2021_01_07_425794 314 5 Shi Shi NNP 10_1101-2021_01_07_425794 314 6 , , , 10_1101-2021_01_07_425794 314 7 Gregory Gregory NNP 10_1101-2021_01_07_425794 314 8 Campbell Campbell NNP 10_1101-2021_01_07_425794 314 9 , , , 10_1101-2021_01_07_425794 314 10 Wendell Wendell NNP 10_1101-2021_01_07_425794 314 11 D D NNP 10_1101-2021_01_07_425794 314 12 Jones Jones NNP 10_1101-2021_01_07_425794 314 13 , , , 10_1101-2021_01_07_425794 314 14 Fabien Fabien NNP 10_1101-2021_01_07_425794 314 15 Campagne Campagne NNP 10_1101-2021_01_07_425794 314 16 , , , 10_1101-2021_01_07_425794 314 17 Zhining Zhining NNP 10_1101-2021_01_07_425794 314 18 Wen Wen NNP 10_1101-2021_01_07_425794 314 19 , , , 10_1101-2021_01_07_425794 314 20 Stephen Stephen NNP 10_1101-2021_01_07_425794 314 21 J J NNP 10_1101-2021_01_07_425794 314 22 Walker Walker NNP 10_1101-2021_01_07_425794 314 23 , , , 10_1101-2021_01_07_425794 314 24 Zhenqiang Zhenqiang NNP 10_1101-2021_01_07_425794 314 25 Su Su NNP 10_1101-2021_01_07_425794 314 26 , , , 10_1101-2021_01_07_425794 314 27 Tzu Tzu NNP 10_1101-2021_01_07_425794 314 28 - - HYPH 10_1101-2021_01_07_425794 314 29 Ming Ming NNP 10_1101-2021_01_07_425794 314 30 Chu Chu NNP 10_1101-2021_01_07_425794 314 31 , , , 10_1101-2021_01_07_425794 314 32 Federico Federico NNP 10_1101-2021_01_07_425794 314 33 M M NNP 10_1101-2021_01_07_425794 314 34 Goodsaid Goodsaid NNP 10_1101-2021_01_07_425794 314 35 , , , 10_1101-2021_01_07_425794 314 36 Lajos Lajos NNP 10_1101-2021_01_07_425794 314 37 Pusz- Pusz- NNP 10_1101-2021_01_07_425794 314 38 tai tai NNP 10_1101-2021_01_07_425794 314 39 , , , 10_1101-2021_01_07_425794 314 40 et et NNP 10_1101-2021_01_07_425794 314 41 al al NNP 10_1101-2021_01_07_425794 314 42 . . . 10_1101-2021_01_07_425794 315 1 The the DT 10_1101-2021_01_07_425794 315 2 MicroArray MicroArray NNP 10_1101-2021_01_07_425794 315 3 Quality Quality NNP 10_1101-2021_01_07_425794 315 4 Control Control NNP 10_1101-2021_01_07_425794 315 5 ( ( -LRB- 10_1101-2021_01_07_425794 315 6 MAQC)-II maqc)-ii JJ 10_1101-2021_01_07_425794 315 7 study study NN 10_1101-2021_01_07_425794 315 8 of of IN 10_1101-2021_01_07_425794 315 9 common common JJ 10_1101-2021_01_07_425794 315 10 practices practice NNS 10_1101-2021_01_07_425794 315 11 for for IN 10_1101-2021_01_07_425794 315 12 the the DT 10_1101-2021_01_07_425794 315 13 development development NN 10_1101-2021_01_07_425794 315 14 and and CC 10_1101-2021_01_07_425794 315 15 validation validation NN 10_1101-2021_01_07_425794 315 16 of of IN 10_1101-2021_01_07_425794 315 17 microarray microarray NNP 10_1101-2021_01_07_425794 315 18 - - HYPH 10_1101-2021_01_07_425794 315 19 based base VBN 10_1101-2021_01_07_425794 315 20 predictive predictive JJ 10_1101-2021_01_07_425794 315 21 models model NNS 10_1101-2021_01_07_425794 315 22 . . . 10_1101-2021_01_07_425794 316 1 Nature Nature NNP 10_1101-2021_01_07_425794 316 2 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425794 316 3 , , , 10_1101-2021_01_07_425794 316 4 28(8):827–838 28(8):827–838 CD 10_1101-2021_01_07_425794 316 5 , , , 10_1101-2021_01_07_425794 316 6 2010 2010 CD 10_1101-2021_01_07_425794 316 7 . . . 10_1101-2021_01_07_425794 317 1 [ [ -LRB- 10_1101-2021_01_07_425794 317 2 15 15 CD 10_1101-2021_01_07_425794 317 3 ] ] -RRB- 10_1101-2021_01_07_425794 317 4 MAQC MAQC NNP 10_1101-2021_01_07_425794 317 5 Consortium Consortium NNP 10_1101-2021_01_07_425794 317 6 , , , 10_1101-2021_01_07_425794 317 7 Leming Leming NNP 10_1101-2021_01_07_425794 317 8 Shi Shi NNP 10_1101-2021_01_07_425794 317 9 , , , 10_1101-2021_01_07_425794 317 10 Laura Laura NNP 10_1101-2021_01_07_425794 317 11 H H NNP 10_1101-2021_01_07_425794 317 12 Reid Reid NNP 10_1101-2021_01_07_425794 317 13 , , , 10_1101-2021_01_07_425794 317 14 Wendell Wendell NNP 10_1101-2021_01_07_425794 317 15 D D NNP 10_1101-2021_01_07_425794 317 16 Jones Jones NNP 10_1101-2021_01_07_425794 317 17 , , , 10_1101-2021_01_07_425794 317 18 Richard Richard NNP 10_1101-2021_01_07_425794 317 19 Shippy Shippy NNP 10_1101-2021_01_07_425794 317 20 , , , 10_1101-2021_01_07_425794 317 21 Janet Janet NNP 10_1101-2021_01_07_425794 317 22 A A NNP 10_1101-2021_01_07_425794 317 23 Warrington Warrington NNP 10_1101-2021_01_07_425794 317 24 , , , 10_1101-2021_01_07_425794 317 25 Shawn Shawn NNP 10_1101-2021_01_07_425794 317 26 C C NNP 10_1101-2021_01_07_425794 317 27 Baker Baker NNP 10_1101-2021_01_07_425794 317 28 , , , 10_1101-2021_01_07_425794 317 29 Patrick Patrick NNP 10_1101-2021_01_07_425794 317 30 J J NNP 10_1101-2021_01_07_425794 317 31 Collins Collins NNP 10_1101-2021_01_07_425794 317 32 , , , 10_1101-2021_01_07_425794 317 33 Francoise Francoise NNP 10_1101-2021_01_07_425794 317 34 de de IN 10_1101-2021_01_07_425794 317 35 Longueville Longueville NNP 10_1101-2021_01_07_425794 317 36 , , , 10_1101-2021_01_07_425794 317 37 Ernest Ernest NNP 10_1101-2021_01_07_425794 317 38 S S NNP 10_1101-2021_01_07_425794 317 39 Kawasaki Kawasaki NNP 10_1101-2021_01_07_425794 317 40 , , , 10_1101-2021_01_07_425794 317 41 et et NNP 10_1101-2021_01_07_425794 317 42 al al NNP 10_1101-2021_01_07_425794 317 43 . . . 10_1101-2021_01_07_425794 318 1 The the DT 10_1101-2021_01_07_425794 318 2 MicroArray MicroArray NNP 10_1101-2021_01_07_425794 318 3 Quality Quality NNP 10_1101-2021_01_07_425794 318 4 Control Control NNP 10_1101-2021_01_07_425794 318 5 ( ( -LRB- 10_1101-2021_01_07_425794 318 6 MAQC MAQC NNP 10_1101-2021_01_07_425794 318 7 ) ) -RRB- 10_1101-2021_01_07_425794 318 8 project project NN 10_1101-2021_01_07_425794 318 9 shows show VBZ 10_1101-2021_01_07_425794 318 10 inter- inter- NN 10_1101-2021_01_07_425794 318 11 and and CC 10_1101-2021_01_07_425794 318 12 intraplatform intraplatform VB 10_1101-2021_01_07_425794 318 13 reproducibility reproducibility NN 10_1101-2021_01_07_425794 318 14 of of IN 10_1101-2021_01_07_425794 318 15 gene gene NN 10_1101-2021_01_07_425794 318 16 expression expression NN 10_1101-2021_01_07_425794 318 17 measurements measurement NNS 10_1101-2021_01_07_425794 318 18 . . . 10_1101-2021_01_07_425794 319 1 Nature Nature NNP 10_1101-2021_01_07_425794 319 2 Biotechnology Biotechnology NNP 10_1101-2021_01_07_425794 319 3 , , , 10_1101-2021_01_07_425794 319 4 24(9):1151–1161 24(9):1151–1161 CD 10_1101-2021_01_07_425794 319 5 , , , 10_1101-2021_01_07_425794 319 6 2006 2006 CD 10_1101-2021_01_07_425794 319 7 . . . 10_1101-2021_01_07_425794 320 1 [ [ -LRB- 10_1101-2021_01_07_425794 320 2 16 16 CD 10_1101-2021_01_07_425794 320 3 ] ] -RRB- 10_1101-2021_01_07_425794 320 4 Yang Yang NNP 10_1101-2021_01_07_425794 320 5 Liao Liao NNP 10_1101-2021_01_07_425794 320 6 and and CC 10_1101-2021_01_07_425794 320 7 Wei Wei NNP 10_1101-2021_01_07_425794 320 8 Shi Shi NNP 10_1101-2021_01_07_425794 320 9 . . . 10_1101-2021_01_07_425794 321 1 seqc seqc NN 10_1101-2021_01_07_425794 321 2 : : : 10_1101-2021_01_07_425794 321 3 RNA RNA NNP 10_1101-2021_01_07_425794 321 4 - - HYPH 10_1101-2021_01_07_425794 321 5 seq seq NN 10_1101-2021_01_07_425794 321 6 data datum NNS 10_1101-2021_01_07_425794 321 7 generated generate VBN 10_1101-2021_01_07_425794 321 8 from from IN 10_1101-2021_01_07_425794 321 9 SEQC SEQC NNP 10_1101-2021_01_07_425794 321 10 ( ( -LRB- 10_1101-2021_01_07_425794 321 11 MAQC MAQC NNP 10_1101-2021_01_07_425794 321 12 - - HYPH 10_1101-2021_01_07_425794 321 13 III III NNP 10_1101-2021_01_07_425794 321 14 ) ) -RRB- 10_1101-2021_01_07_425794 321 15 study study NN 10_1101-2021_01_07_425794 321 16 , , , 10_1101-2021_01_07_425794 321 17 2020 2020 CD 10_1101-2021_01_07_425794 321 18 . . . 10_1101-2021_01_07_425794 322 1 R r NN 10_1101-2021_01_07_425794 322 2 package package NN 10_1101-2021_01_07_425794 322 3 version version NN 10_1101-2021_01_07_425794 322 4 1.22.0 1.22.0 CD 10_1101-2021_01_07_425794 322 5 . . . 10_1101-2021_01_07_425794 322 6 http://bioconductor.org/packages/release/data/experiment/html/seqc.html http://bioconductor.org/packages/release/data/experiment/html/seqc.html NNP 10_1101-2021_01_07_425794 322 7 . . . 10_1101-2021_01_07_425794 323 1 22 22 CD 10_1101-2021_01_07_425794 323 2 .CC .CC : 10_1101-2021_01_07_425794 323 3 - - HYPH 10_1101-2021_01_07_425794 323 4 BY by IN 10_1101-2021_01_07_425794 323 5 - - HYPH 10_1101-2021_01_07_425794 323 6 NC NC NNP 10_1101-2021_01_07_425794 323 7 - - HYPH 10_1101-2021_01_07_425794 323 8 ND ND NNP 10_1101-2021_01_07_425794 323 9 4.0 4.0 CD 10_1101-2021_01_07_425794 323 10 International international JJ 10_1101-2021_01_07_425794 323 11 licensemade licensemade NN 10_1101-2021_01_07_425794 323 12 available available JJ 10_1101-2021_01_07_425794 323 13 under under IN 10_1101-2021_01_07_425794 323 14 a a DT 10_1101-2021_01_07_425794 323 15 ( ( -LRB- 10_1101-2021_01_07_425794 323 16 which which WDT 10_1101-2021_01_07_425794 323 17 was be VBD 10_1101-2021_01_07_425794 323 18 not not RB 10_1101-2021_01_07_425794 323 19 certified certify VBN 10_1101-2021_01_07_425794 323 20 by by IN 10_1101-2021_01_07_425794 323 21 peer peer NN 10_1101-2021_01_07_425794 323 22 review review NN 10_1101-2021_01_07_425794 323 23 ) ) -RRB- 10_1101-2021_01_07_425794 323 24 is be VBZ 10_1101-2021_01_07_425794 323 25 the the DT 10_1101-2021_01_07_425794 323 26 author author NN 10_1101-2021_01_07_425794 323 27 / / SYM 10_1101-2021_01_07_425794 323 28 funder funder NN 10_1101-2021_01_07_425794 323 29 , , , 10_1101-2021_01_07_425794 323 30 who who WP 10_1101-2021_01_07_425794 323 31 has have VBZ 10_1101-2021_01_07_425794 323 32 granted grant VBN 10_1101-2021_01_07_425794 323 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 323 34 a a DT 10_1101-2021_01_07_425794 323 35 license license NN 10_1101-2021_01_07_425794 323 36 to to TO 10_1101-2021_01_07_425794 323 37 display display VB 10_1101-2021_01_07_425794 323 38 the the DT 10_1101-2021_01_07_425794 323 39 preprint preprint NN 10_1101-2021_01_07_425794 323 40 in in IN 10_1101-2021_01_07_425794 323 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 323 42 . . . 10_1101-2021_01_07_425794 324 1 It -PRON- PRP 10_1101-2021_01_07_425794 324 2 is be VBZ 10_1101-2021_01_07_425794 324 3 The the DT 10_1101-2021_01_07_425794 324 4 copyright copyright NN 10_1101-2021_01_07_425794 324 5 holder holder NN 10_1101-2021_01_07_425794 324 6 for for IN 10_1101-2021_01_07_425794 324 7 this this DT 10_1101-2021_01_07_425794 324 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 324 9 version version NN 10_1101-2021_01_07_425794 324 10 posted post VBD 10_1101-2021_01_07_425794 324 11 January January NNP 10_1101-2021_01_07_425794 324 12 8 8 CD 10_1101-2021_01_07_425794 324 13 , , , 10_1101-2021_01_07_425794 324 14 2021 2021 CD 10_1101-2021_01_07_425794 324 15 . . . 10_1101-2021_01_07_425794 324 16 ; ; : 10_1101-2021_01_07_425794 324 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 324 18 : : : 10_1101-2021_01_07_425794 324 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 324 20 preprint preprint JJ 10_1101-2021_01_07_425794 324 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 324 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 324 23 [ [ -LRB- 10_1101-2021_01_07_425794 324 24 17 17 CD 10_1101-2021_01_07_425794 324 25 ] ] -RRB- 10_1101-2021_01_07_425794 324 26 Marc Marc NNP 10_1101-2021_01_07_425794 324 27 Carlson Carlson NNP 10_1101-2021_01_07_425794 324 28 . . . 10_1101-2021_01_07_425794 325 1 org.Hs.eg.db org.hs.eg.db LS 10_1101-2021_01_07_425794 325 2 : : : 10_1101-2021_01_07_425794 325 3 Genome genome VB 10_1101-2021_01_07_425794 325 4 wide wide JJ 10_1101-2021_01_07_425794 325 5 annota- annota- JJ 10_1101-2021_01_07_425794 325 6 tion tion NN 10_1101-2021_01_07_425794 325 7 for for IN 10_1101-2021_01_07_425794 325 8 Human Human NNP 10_1101-2021_01_07_425794 325 9 , , , 10_1101-2021_01_07_425794 325 10 2020 2020 CD 10_1101-2021_01_07_425794 325 11 . . . 10_1101-2021_01_07_425794 326 1 R r NN 10_1101-2021_01_07_425794 326 2 package package NN 10_1101-2021_01_07_425794 326 3 version version NN 10_1101-2021_01_07_425794 326 4 3.11.4 3.11.4 CD 10_1101-2021_01_07_425794 326 5 . . . 10_1101-2021_01_07_425794 326 6 https://www.bioconductor.org/packages/release/data/annotation/html/org.Hs.eg.db.html https://www.bioconductor.org/packages/release/data/annotation/html/org.Hs.eg.db.html NNP 10_1101-2021_01_07_425794 326 7 . . . 10_1101-2021_01_07_425794 327 1 [ [ -LRB- 10_1101-2021_01_07_425794 327 2 18 18 CD 10_1101-2021_01_07_425794 327 3 ] ] -RRB- 10_1101-2021_01_07_425794 327 4 Matthew Matthew NNP 10_1101-2021_01_07_425794 327 5 E E NNP 10_1101-2021_01_07_425794 327 6 Ritchie Ritchie NNP 10_1101-2021_01_07_425794 327 7 , , , 10_1101-2021_01_07_425794 327 8 Belinda Belinda NNP 10_1101-2021_01_07_425794 327 9 Phipson Phipson NNP 10_1101-2021_01_07_425794 327 10 , , , 10_1101-2021_01_07_425794 327 11 Di Di NNP 10_1101-2021_01_07_425794 327 12 Wu Wu NNP 10_1101-2021_01_07_425794 327 13 , , , 10_1101-2021_01_07_425794 327 14 Yifang Yifang NNP 10_1101-2021_01_07_425794 327 15 Hu Hu NNP 10_1101-2021_01_07_425794 327 16 , , , 10_1101-2021_01_07_425794 327 17 Charity Charity NNP 10_1101-2021_01_07_425794 327 18 W W NNP 10_1101-2021_01_07_425794 327 19 Law Law NNP 10_1101-2021_01_07_425794 327 20 , , , 10_1101-2021_01_07_425794 327 21 Wei Wei NNP 10_1101-2021_01_07_425794 327 22 Shi Shi NNP 10_1101-2021_01_07_425794 327 23 , , , 10_1101-2021_01_07_425794 327 24 and and CC 10_1101-2021_01_07_425794 327 25 Gordon Gordon NNP 10_1101-2021_01_07_425794 327 26 K K NNP 10_1101-2021_01_07_425794 327 27 Smyth Smyth NNP 10_1101-2021_01_07_425794 327 28 . . . 10_1101-2021_01_07_425794 328 1 limma limma NNP 10_1101-2021_01_07_425794 328 2 powers powers NNP 10_1101-2021_01_07_425794 328 3 differential differential VBP 10_1101-2021_01_07_425794 328 4 expression expression NN 10_1101-2021_01_07_425794 328 5 analyses analysis NNS 10_1101-2021_01_07_425794 328 6 for for IN 10_1101-2021_01_07_425794 328 7 RNA- RNA- NNP 10_1101-2021_01_07_425794 328 8 sequencing sequencing NN 10_1101-2021_01_07_425794 328 9 and and CC 10_1101-2021_01_07_425794 328 10 microarray microarray JJ 10_1101-2021_01_07_425794 328 11 studies study NNS 10_1101-2021_01_07_425794 328 12 . . . 10_1101-2021_01_07_425794 329 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 329 2 Acids Acids NNPS 10_1101-2021_01_07_425794 329 3 Research Research NNP 10_1101-2021_01_07_425794 329 4 , , , 10_1101-2021_01_07_425794 329 5 43(7):e47–e47 43(7):e47–e47 CD 10_1101-2021_01_07_425794 329 6 , , , 10_1101-2021_01_07_425794 329 7 2015 2015 CD 10_1101-2021_01_07_425794 329 8 . . . 10_1101-2021_01_07_425794 330 1 [ [ -LRB- 10_1101-2021_01_07_425794 330 2 19 19 CD 10_1101-2021_01_07_425794 330 3 ] ] -RRB- 10_1101-2021_01_07_425794 330 4 Wolfgang Wolfgang NNP 10_1101-2021_01_07_425794 330 5 Huber Huber NNP 10_1101-2021_01_07_425794 330 6 , , , 10_1101-2021_01_07_425794 330 7 Vincent Vincent NNP 10_1101-2021_01_07_425794 330 8 J J NNP 10_1101-2021_01_07_425794 330 9 Carey Carey NNP 10_1101-2021_01_07_425794 330 10 , , , 10_1101-2021_01_07_425794 330 11 Robert Robert NNP 10_1101-2021_01_07_425794 330 12 Gentleman Gentleman NNP 10_1101-2021_01_07_425794 330 13 , , , 10_1101-2021_01_07_425794 330 14 Simon Simon NNP 10_1101-2021_01_07_425794 330 15 Anders Anders NNP 10_1101-2021_01_07_425794 330 16 , , , 10_1101-2021_01_07_425794 330 17 Marc Marc NNP 10_1101-2021_01_07_425794 330 18 Carlson Carlson NNP 10_1101-2021_01_07_425794 330 19 , , , 10_1101-2021_01_07_425794 330 20 Benilton Benilton NNP 10_1101-2021_01_07_425794 330 21 S S NNP 10_1101-2021_01_07_425794 330 22 Carvalho Carvalho NNP 10_1101-2021_01_07_425794 330 23 , , , 10_1101-2021_01_07_425794 330 24 Hector Hector NNP 10_1101-2021_01_07_425794 330 25 Corrada Corrada NNP 10_1101-2021_01_07_425794 330 26 Bravo Bravo NNP 10_1101-2021_01_07_425794 330 27 , , , 10_1101-2021_01_07_425794 330 28 Sean Sean NNP 10_1101-2021_01_07_425794 330 29 Davis Davis NNP 10_1101-2021_01_07_425794 330 30 , , , 10_1101-2021_01_07_425794 330 31 Laurent Laurent NNP 10_1101-2021_01_07_425794 330 32 Gatto Gatto NNP 10_1101-2021_01_07_425794 330 33 , , , 10_1101-2021_01_07_425794 330 34 Thomas Thomas NNP 10_1101-2021_01_07_425794 330 35 Girke Girke NNP 10_1101-2021_01_07_425794 330 36 , , , 10_1101-2021_01_07_425794 330 37 et et NNP 10_1101-2021_01_07_425794 330 38 al al NNP 10_1101-2021_01_07_425794 330 39 . . . 10_1101-2021_01_07_425794 331 1 Orchestrating orchestrate VBG 10_1101-2021_01_07_425794 331 2 high high JJ 10_1101-2021_01_07_425794 331 3 - - HYPH 10_1101-2021_01_07_425794 331 4 throughput throughput NN 10_1101-2021_01_07_425794 331 5 genomic genomic JJ 10_1101-2021_01_07_425794 331 6 analysis analysis NN 10_1101-2021_01_07_425794 331 7 with with IN 10_1101-2021_01_07_425794 331 8 Bioconductor Bioconductor NNP 10_1101-2021_01_07_425794 331 9 . . . 10_1101-2021_01_07_425794 332 1 Nature Nature NNP 10_1101-2021_01_07_425794 332 2 Methods Methods NNPS 10_1101-2021_01_07_425794 332 3 , , , 10_1101-2021_01_07_425794 332 4 12(2):115 12(2):115 CD 10_1101-2021_01_07_425794 332 5 , , , 10_1101-2021_01_07_425794 332 6 2015 2015 CD 10_1101-2021_01_07_425794 332 7 . . . 10_1101-2021_01_07_425794 333 1 [ [ -LRB- 10_1101-2021_01_07_425794 333 2 20 20 CD 10_1101-2021_01_07_425794 333 3 ] ] -RRB- 10_1101-2021_01_07_425794 333 4 Yang Yang NNP 10_1101-2021_01_07_425794 333 5 Liao Liao NNP 10_1101-2021_01_07_425794 333 6 , , , 10_1101-2021_01_07_425794 333 7 Gordon Gordon NNP 10_1101-2021_01_07_425794 333 8 K K NNP 10_1101-2021_01_07_425794 333 9 Smyth Smyth NNP 10_1101-2021_01_07_425794 333 10 , , , 10_1101-2021_01_07_425794 333 11 and and CC 10_1101-2021_01_07_425794 333 12 Wei Wei NNP 10_1101-2021_01_07_425794 333 13 Shi Shi NNP 10_1101-2021_01_07_425794 333 14 . . . 10_1101-2021_01_07_425794 334 1 The the DT 10_1101-2021_01_07_425794 334 2 Subread Subread NNP 10_1101-2021_01_07_425794 334 3 aligner aligner NN 10_1101-2021_01_07_425794 334 4 : : : 10_1101-2021_01_07_425794 334 5 fast fast RB 10_1101-2021_01_07_425794 334 6 , , , 10_1101-2021_01_07_425794 334 7 accurate accurate JJ 10_1101-2021_01_07_425794 334 8 and and CC 10_1101-2021_01_07_425794 334 9 scalable scalable JJ 10_1101-2021_01_07_425794 334 10 read read VBN 10_1101-2021_01_07_425794 334 11 mapping mapping NN 10_1101-2021_01_07_425794 334 12 by by IN 10_1101-2021_01_07_425794 334 13 seed seed NN 10_1101-2021_01_07_425794 334 14 - - HYPH 10_1101-2021_01_07_425794 334 15 and and CC 10_1101-2021_01_07_425794 334 16 - - HYPH 10_1101-2021_01_07_425794 334 17 vote vote NN 10_1101-2021_01_07_425794 334 18 . . . 10_1101-2021_01_07_425794 335 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 335 2 Acids Acids NNPS 10_1101-2021_01_07_425794 335 3 Research Research NNP 10_1101-2021_01_07_425794 335 4 , , , 10_1101-2021_01_07_425794 335 5 41(10):e108–e108 41(10):e108–e108 CD 10_1101-2021_01_07_425794 335 6 , , , 10_1101-2021_01_07_425794 335 7 2013 2013 CD 10_1101-2021_01_07_425794 335 8 . . . 10_1101-2021_01_07_425794 336 1 [ [ -LRB- 10_1101-2021_01_07_425794 336 2 21 21 CD 10_1101-2021_01_07_425794 336 3 ] ] -RRB- 10_1101-2021_01_07_425794 336 4 Charity Charity NNP 10_1101-2021_01_07_425794 336 5 W W NNP 10_1101-2021_01_07_425794 336 6 Law Law NNP 10_1101-2021_01_07_425794 336 7 , , , 10_1101-2021_01_07_425794 336 8 Yunshun Yunshun NNP 10_1101-2021_01_07_425794 336 9 Chen Chen NNP 10_1101-2021_01_07_425794 336 10 , , , 10_1101-2021_01_07_425794 336 11 Wei Wei NNP 10_1101-2021_01_07_425794 336 12 Shi Shi NNP 10_1101-2021_01_07_425794 336 13 , , , 10_1101-2021_01_07_425794 336 14 and and CC 10_1101-2021_01_07_425794 336 15 Gordon Gordon NNP 10_1101-2021_01_07_425794 336 16 K K NNP 10_1101-2021_01_07_425794 336 17 Smyth Smyth NNP 10_1101-2021_01_07_425794 336 18 . . . 10_1101-2021_01_07_425794 337 1 voom voom NNP 10_1101-2021_01_07_425794 337 2 : : : 10_1101-2021_01_07_425794 337 3 Precision precision NN 10_1101-2021_01_07_425794 337 4 weights weight NNS 10_1101-2021_01_07_425794 337 5 unlock unlock VBP 10_1101-2021_01_07_425794 337 6 linear linear JJ 10_1101-2021_01_07_425794 337 7 model model NN 10_1101-2021_01_07_425794 337 8 analysis analysis NN 10_1101-2021_01_07_425794 337 9 tools tool NNS 10_1101-2021_01_07_425794 337 10 for for IN 10_1101-2021_01_07_425794 337 11 RNA RNA NNP 10_1101-2021_01_07_425794 337 12 - - HYPH 10_1101-2021_01_07_425794 337 13 seq seq NN 10_1101-2021_01_07_425794 337 14 read read VB 10_1101-2021_01_07_425794 337 15 counts count NNS 10_1101-2021_01_07_425794 337 16 . . . 10_1101-2021_01_07_425794 338 1 Genome Genome NNP 10_1101-2021_01_07_425794 338 2 Biology Biology NNP 10_1101-2021_01_07_425794 338 3 , , , 10_1101-2021_01_07_425794 338 4 15(2):R29 15(2):r29 CD 10_1101-2021_01_07_425794 338 5 , , , 10_1101-2021_01_07_425794 338 6 2014 2014 CD 10_1101-2021_01_07_425794 338 7 . . . 10_1101-2021_01_07_425794 339 1 [ [ -LRB- 10_1101-2021_01_07_425794 339 2 22 22 CD 10_1101-2021_01_07_425794 339 3 ] ] -RRB- 10_1101-2021_01_07_425794 339 4 Ali Ali NNP 10_1101-2021_01_07_425794 339 5 Mortazavi Mortazavi NNP 10_1101-2021_01_07_425794 339 6 , , , 10_1101-2021_01_07_425794 339 7 Brian Brian NNP 10_1101-2021_01_07_425794 339 8 A A NNP 10_1101-2021_01_07_425794 339 9 Williams Williams NNP 10_1101-2021_01_07_425794 339 10 , , , 10_1101-2021_01_07_425794 339 11 Kenneth Kenneth NNP 10_1101-2021_01_07_425794 339 12 McCue McCue NNP 10_1101-2021_01_07_425794 339 13 , , , 10_1101-2021_01_07_425794 339 14 Lorian Lorian NNP 10_1101-2021_01_07_425794 339 15 Schaeffer Schaeffer NNP 10_1101-2021_01_07_425794 339 16 , , , 10_1101-2021_01_07_425794 339 17 and and CC 10_1101-2021_01_07_425794 339 18 Barbara Barbara NNP 10_1101-2021_01_07_425794 339 19 Wold Wold NNP 10_1101-2021_01_07_425794 339 20 . . . 10_1101-2021_01_07_425794 340 1 Mapping mapping NN 10_1101-2021_01_07_425794 340 2 and and CC 10_1101-2021_01_07_425794 340 3 quantifying quantify VBG 10_1101-2021_01_07_425794 340 4 mammalian mammalian JJ 10_1101-2021_01_07_425794 340 5 transcriptomes transcriptome NNS 10_1101-2021_01_07_425794 340 6 by by IN 10_1101-2021_01_07_425794 340 7 RNA RNA NNP 10_1101-2021_01_07_425794 340 8 - - HYPH 10_1101-2021_01_07_425794 340 9 Seq Seq NNP 10_1101-2021_01_07_425794 340 10 . . . 10_1101-2021_01_07_425794 341 1 Nat Nat NNP 10_1101-2021_01_07_425794 341 2 Methods Methods NNP 10_1101-2021_01_07_425794 341 3 , , , 10_1101-2021_01_07_425794 341 4 5(7):621–8 5(7):621–8 NNP 10_1101-2021_01_07_425794 341 5 , , , 10_1101-2021_01_07_425794 341 6 2008 2008 CD 10_1101-2021_01_07_425794 341 7 . . . 10_1101-2021_01_07_425794 342 1 [ [ -LRB- 10_1101-2021_01_07_425794 342 2 23 23 CD 10_1101-2021_01_07_425794 342 3 ] ] -RRB- 10_1101-2021_01_07_425794 342 4 Benjamin Benjamin NNP 10_1101-2021_01_07_425794 342 5 M M NNP 10_1101-2021_01_07_425794 342 6 Bolstad Bolstad NNP 10_1101-2021_01_07_425794 342 7 , , , 10_1101-2021_01_07_425794 342 8 Rafael Rafael NNP 10_1101-2021_01_07_425794 342 9 A A NNP 10_1101-2021_01_07_425794 342 10 Irizarry Irizarry NNP 10_1101-2021_01_07_425794 342 11 , , , 10_1101-2021_01_07_425794 342 12 Magnus Magnus NNP 10_1101-2021_01_07_425794 342 13 Åstrand Åstrand NNP 10_1101-2021_01_07_425794 342 14 , , , 10_1101-2021_01_07_425794 342 15 and and CC 10_1101-2021_01_07_425794 342 16 Terence Terence NNP 10_1101-2021_01_07_425794 342 17 P. P. NNP 10_1101-2021_01_07_425794 342 18 Speed Speed NNP 10_1101-2021_01_07_425794 342 19 . . . 10_1101-2021_01_07_425794 343 1 A a DT 10_1101-2021_01_07_425794 343 2 comparison comparison NN 10_1101-2021_01_07_425794 343 3 of of IN 10_1101-2021_01_07_425794 343 4 normalization normalization NN 10_1101-2021_01_07_425794 343 5 methods method NNS 10_1101-2021_01_07_425794 343 6 for for IN 10_1101-2021_01_07_425794 343 7 high high JJ 10_1101-2021_01_07_425794 343 8 density density NN 10_1101-2021_01_07_425794 343 9 oligonucleotide oligonucleotide IN 10_1101-2021_01_07_425794 343 10 array array NNP 10_1101-2021_01_07_425794 343 11 data datum NNS 10_1101-2021_01_07_425794 343 12 based base VBN 10_1101-2021_01_07_425794 343 13 on on IN 10_1101-2021_01_07_425794 343 14 variance variance NN 10_1101-2021_01_07_425794 343 15 and and CC 10_1101-2021_01_07_425794 343 16 bias bias NN 10_1101-2021_01_07_425794 343 17 . . . 10_1101-2021_01_07_425794 344 1 Bioinformatics bioinformatic NNS 10_1101-2021_01_07_425794 344 2 , , , 10_1101-2021_01_07_425794 344 3 19(2):185–193 19(2):185–193 CD 10_1101-2021_01_07_425794 344 4 , , , 10_1101-2021_01_07_425794 344 5 2003 2003 CD 10_1101-2021_01_07_425794 344 6 . . . 10_1101-2021_01_07_425794 345 1 [ [ -LRB- 10_1101-2021_01_07_425794 345 2 24 24 CD 10_1101-2021_01_07_425794 345 3 ] ] -RRB- 10_1101-2021_01_07_425794 345 4 Mark Mark NNP 10_1101-2021_01_07_425794 345 5 D D NNP 10_1101-2021_01_07_425794 345 6 Robinson Robinson NNP 10_1101-2021_01_07_425794 345 7 and and CC 10_1101-2021_01_07_425794 345 8 Alicia Alicia NNP 10_1101-2021_01_07_425794 345 9 Oshlack Oshlack NNP 10_1101-2021_01_07_425794 345 10 . . . 10_1101-2021_01_07_425794 346 1 A a DT 10_1101-2021_01_07_425794 346 2 scaling scale VBG 10_1101-2021_01_07_425794 346 3 normalization normalization NN 10_1101-2021_01_07_425794 346 4 method method NN 10_1101-2021_01_07_425794 346 5 for for IN 10_1101-2021_01_07_425794 346 6 differen- differen- NNP 10_1101-2021_01_07_425794 346 7 tial tial JJ 10_1101-2021_01_07_425794 346 8 expression expression NN 10_1101-2021_01_07_425794 346 9 analysis analysis NN 10_1101-2021_01_07_425794 346 10 of of IN 10_1101-2021_01_07_425794 346 11 RNA RNA NNP 10_1101-2021_01_07_425794 346 12 - - HYPH 10_1101-2021_01_07_425794 346 13 seq seq NN 10_1101-2021_01_07_425794 346 14 data datum NNS 10_1101-2021_01_07_425794 346 15 . . . 10_1101-2021_01_07_425794 347 1 Genome Genome NNP 10_1101-2021_01_07_425794 347 2 Biology Biology NNP 10_1101-2021_01_07_425794 347 3 , , , 10_1101-2021_01_07_425794 347 4 11(3):R25 11(3):r25 CD 10_1101-2021_01_07_425794 347 5 , , , 10_1101-2021_01_07_425794 347 6 2010 2010 CD 10_1101-2021_01_07_425794 347 7 . . . 10_1101-2021_01_07_425794 348 1 [ [ -LRB- 10_1101-2021_01_07_425794 348 2 25 25 CD 10_1101-2021_01_07_425794 348 3 ] ] -RRB- 10_1101-2021_01_07_425794 348 4 Mark Mark NNP 10_1101-2021_01_07_425794 348 5 D D NNP 10_1101-2021_01_07_425794 348 6 Robinson Robinson NNP 10_1101-2021_01_07_425794 348 7 , , , 10_1101-2021_01_07_425794 348 8 Davis Davis NNP 10_1101-2021_01_07_425794 348 9 J J NNP 10_1101-2021_01_07_425794 348 10 McCarthy McCarthy NNP 10_1101-2021_01_07_425794 348 11 , , , 10_1101-2021_01_07_425794 348 12 and and CC 10_1101-2021_01_07_425794 348 13 Gordon Gordon NNP 10_1101-2021_01_07_425794 348 14 K K NNP 10_1101-2021_01_07_425794 348 15 Smyth Smyth NNP 10_1101-2021_01_07_425794 348 16 . . . 10_1101-2021_01_07_425794 349 1 edgeR edgeR NNP 10_1101-2021_01_07_425794 349 2 : : : 10_1101-2021_01_07_425794 349 3 a a DT 10_1101-2021_01_07_425794 349 4 Biocon- Biocon- NNP 10_1101-2021_01_07_425794 349 5 ductor ductor NN 10_1101-2021_01_07_425794 349 6 package package NN 10_1101-2021_01_07_425794 349 7 for for IN 10_1101-2021_01_07_425794 349 8 differential differential NN 10_1101-2021_01_07_425794 349 9 expression expression NN 10_1101-2021_01_07_425794 349 10 analysis analysis NN 10_1101-2021_01_07_425794 349 11 of of IN 10_1101-2021_01_07_425794 349 12 digital digital NNP 10_1101-2021_01_07_425794 349 13 gene gene NN 10_1101-2021_01_07_425794 349 14 expression expression NNP 10_1101-2021_01_07_425794 349 15 data datum NNS 10_1101-2021_01_07_425794 349 16 . . . 10_1101-2021_01_07_425794 350 1 Bioinformatics bioinformatic NNS 10_1101-2021_01_07_425794 350 2 , , , 10_1101-2021_01_07_425794 350 3 26(1):139–40 26(1):139–40 CD 10_1101-2021_01_07_425794 350 4 , , , 10_1101-2021_01_07_425794 350 5 2010 2010 CD 10_1101-2021_01_07_425794 350 6 . . . 10_1101-2021_01_07_425794 351 1 [ [ -LRB- 10_1101-2021_01_07_425794 351 2 26 26 CD 10_1101-2021_01_07_425794 351 3 ] ] -RRB- 10_1101-2021_01_07_425794 351 4 Wei Wei NNP 10_1101-2021_01_07_425794 351 5 Shi Shi NNP 10_1101-2021_01_07_425794 351 6 , , , 10_1101-2021_01_07_425794 351 7 Alicia Alicia NNP 10_1101-2021_01_07_425794 351 8 Oshlack Oshlack NNP 10_1101-2021_01_07_425794 351 9 , , , 10_1101-2021_01_07_425794 351 10 and and CC 10_1101-2021_01_07_425794 351 11 Gordon Gordon NNP 10_1101-2021_01_07_425794 351 12 K K NNP 10_1101-2021_01_07_425794 351 13 Smyth Smyth NNP 10_1101-2021_01_07_425794 351 14 . . . 10_1101-2021_01_07_425794 352 1 Optimizing optimize VBG 10_1101-2021_01_07_425794 352 2 the the DT 10_1101-2021_01_07_425794 352 3 noise noise NN 10_1101-2021_01_07_425794 352 4 versus versus IN 10_1101-2021_01_07_425794 352 5 bias bias NNP 10_1101-2021_01_07_425794 352 6 trade trade NN 10_1101-2021_01_07_425794 352 7 - - HYPH 10_1101-2021_01_07_425794 352 8 off off NN 10_1101-2021_01_07_425794 352 9 for for IN 10_1101-2021_01_07_425794 352 10 Illumina Illumina NNP 10_1101-2021_01_07_425794 352 11 whole whole JJ 10_1101-2021_01_07_425794 352 12 genome genome JJ 10_1101-2021_01_07_425794 352 13 expression expression NN 10_1101-2021_01_07_425794 352 14 BeadChips BeadChips NNP 10_1101-2021_01_07_425794 352 15 . . . 10_1101-2021_01_07_425794 353 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 353 2 Acids Acids NNP 10_1101-2021_01_07_425794 353 3 Research Research NNP 10_1101-2021_01_07_425794 353 4 , , , 10_1101-2021_01_07_425794 353 5 38(22):e204 38(22):e204 NNP 10_1101-2021_01_07_425794 353 6 , , , 10_1101-2021_01_07_425794 353 7 2010 2010 CD 10_1101-2021_01_07_425794 353 8 . . . 10_1101-2021_01_07_425794 354 1 [ [ -LRB- 10_1101-2021_01_07_425794 354 2 27 27 CD 10_1101-2021_01_07_425794 354 3 ] ] -RRB- 10_1101-2021_01_07_425794 354 4 Kim Kim NNP 10_1101-2021_01_07_425794 354 5 D D NNP 10_1101-2021_01_07_425794 354 6 Pruitt Pruitt NNP 10_1101-2021_01_07_425794 354 7 , , , 10_1101-2021_01_07_425794 354 8 Garth Garth NNP 10_1101-2021_01_07_425794 354 9 R R NNP 10_1101-2021_01_07_425794 354 10 Brown Brown NNP 10_1101-2021_01_07_425794 354 11 , , , 10_1101-2021_01_07_425794 354 12 Susan Susan NNP 10_1101-2021_01_07_425794 354 13 M M NNP 10_1101-2021_01_07_425794 354 14 Hiatt Hiatt NNP 10_1101-2021_01_07_425794 354 15 , , , 10_1101-2021_01_07_425794 354 16 Françoise françoise CD 10_1101-2021_01_07_425794 354 17 Thibaud Thibaud NNP 10_1101-2021_01_07_425794 354 18 - - HYPH 10_1101-2021_01_07_425794 354 19 Nissen Nissen NNP 10_1101-2021_01_07_425794 354 20 , , , 10_1101-2021_01_07_425794 354 21 Alexander Alexander NNP 10_1101-2021_01_07_425794 354 22 Astashyn Astashyn NNP 10_1101-2021_01_07_425794 354 23 , , , 10_1101-2021_01_07_425794 354 24 Olga Olga NNP 10_1101-2021_01_07_425794 354 25 Ermolaeva Ermolaeva NNP 10_1101-2021_01_07_425794 354 26 , , , 10_1101-2021_01_07_425794 354 27 Catherine Catherine NNP 10_1101-2021_01_07_425794 354 28 M M NNP 10_1101-2021_01_07_425794 354 29 Farrell Farrell NNP 10_1101-2021_01_07_425794 354 30 , , , 10_1101-2021_01_07_425794 354 31 Jennifer Jennifer NNP 10_1101-2021_01_07_425794 354 32 Hart Hart NNP 10_1101-2021_01_07_425794 354 33 , , , 10_1101-2021_01_07_425794 354 34 Melissa Melissa NNP 10_1101-2021_01_07_425794 354 35 J J NNP 10_1101-2021_01_07_425794 354 36 Landrum Landrum NNP 10_1101-2021_01_07_425794 354 37 , , , 10_1101-2021_01_07_425794 354 38 23 23 CD 10_1101-2021_01_07_425794 354 39 .CC .cc SYM 10_1101-2021_01_07_425794 354 40 - - HYPH 10_1101-2021_01_07_425794 354 41 BY by IN 10_1101-2021_01_07_425794 354 42 - - HYPH 10_1101-2021_01_07_425794 354 43 NC NC NNP 10_1101-2021_01_07_425794 354 44 - - HYPH 10_1101-2021_01_07_425794 354 45 ND ND NNP 10_1101-2021_01_07_425794 354 46 4.0 4.0 CD 10_1101-2021_01_07_425794 354 47 International international JJ 10_1101-2021_01_07_425794 354 48 licensemade licensemade NN 10_1101-2021_01_07_425794 354 49 available available JJ 10_1101-2021_01_07_425794 354 50 under under IN 10_1101-2021_01_07_425794 354 51 a a DT 10_1101-2021_01_07_425794 354 52 ( ( -LRB- 10_1101-2021_01_07_425794 354 53 which which WDT 10_1101-2021_01_07_425794 354 54 was be VBD 10_1101-2021_01_07_425794 354 55 not not RB 10_1101-2021_01_07_425794 354 56 certified certify VBN 10_1101-2021_01_07_425794 354 57 by by IN 10_1101-2021_01_07_425794 354 58 peer peer NN 10_1101-2021_01_07_425794 354 59 review review NN 10_1101-2021_01_07_425794 354 60 ) ) -RRB- 10_1101-2021_01_07_425794 354 61 is be VBZ 10_1101-2021_01_07_425794 354 62 the the DT 10_1101-2021_01_07_425794 354 63 author author NN 10_1101-2021_01_07_425794 354 64 / / SYM 10_1101-2021_01_07_425794 354 65 funder funder NN 10_1101-2021_01_07_425794 354 66 , , , 10_1101-2021_01_07_425794 354 67 who who WP 10_1101-2021_01_07_425794 354 68 has have VBZ 10_1101-2021_01_07_425794 354 69 granted grant VBN 10_1101-2021_01_07_425794 354 70 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 354 71 a a DT 10_1101-2021_01_07_425794 354 72 license license NN 10_1101-2021_01_07_425794 354 73 to to TO 10_1101-2021_01_07_425794 354 74 display display VB 10_1101-2021_01_07_425794 354 75 the the DT 10_1101-2021_01_07_425794 354 76 preprint preprint NN 10_1101-2021_01_07_425794 354 77 in in IN 10_1101-2021_01_07_425794 354 78 perpetuity perpetuity NN 10_1101-2021_01_07_425794 354 79 . . . 10_1101-2021_01_07_425794 355 1 It -PRON- PRP 10_1101-2021_01_07_425794 355 2 is be VBZ 10_1101-2021_01_07_425794 355 3 The the DT 10_1101-2021_01_07_425794 355 4 copyright copyright NN 10_1101-2021_01_07_425794 355 5 holder holder NN 10_1101-2021_01_07_425794 355 6 for for IN 10_1101-2021_01_07_425794 355 7 this this DT 10_1101-2021_01_07_425794 355 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 355 9 version version NN 10_1101-2021_01_07_425794 355 10 posted post VBD 10_1101-2021_01_07_425794 355 11 January January NNP 10_1101-2021_01_07_425794 355 12 8 8 CD 10_1101-2021_01_07_425794 355 13 , , , 10_1101-2021_01_07_425794 355 14 2021 2021 CD 10_1101-2021_01_07_425794 355 15 . . . 10_1101-2021_01_07_425794 355 16 ; ; : 10_1101-2021_01_07_425794 355 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 355 18 : : : 10_1101-2021_01_07_425794 355 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 355 20 preprint preprint JJ 10_1101-2021_01_07_425794 355 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 355 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN 10_1101-2021_01_07_425794 355 23 Kelly Kelly NNP 10_1101-2021_01_07_425794 355 24 M M NNP 10_1101-2021_01_07_425794 355 25 McGarvey McGarvey NNP 10_1101-2021_01_07_425794 355 26 , , , 10_1101-2021_01_07_425794 355 27 et et NNP 10_1101-2021_01_07_425794 355 28 al al NNP 10_1101-2021_01_07_425794 355 29 . . . 10_1101-2021_01_07_425794 356 1 RefSeq refseq NN 10_1101-2021_01_07_425794 356 2 : : : 10_1101-2021_01_07_425794 356 3 an an DT 10_1101-2021_01_07_425794 356 4 update update NN 10_1101-2021_01_07_425794 356 5 on on IN 10_1101-2021_01_07_425794 356 6 mammalian mammalian JJ 10_1101-2021_01_07_425794 356 7 reference reference NN 10_1101-2021_01_07_425794 356 8 sequences sequence NNS 10_1101-2021_01_07_425794 356 9 . . . 10_1101-2021_01_07_425794 357 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425794 357 2 Acids Acids NNP 10_1101-2021_01_07_425794 357 3 Research Research NNP 10_1101-2021_01_07_425794 357 4 , , , 10_1101-2021_01_07_425794 357 5 42(Database 42(database CD 10_1101-2021_01_07_425794 357 6 issue):D756–D763 issue):d756–d763 UH 10_1101-2021_01_07_425794 357 7 , , , 10_1101-2021_01_07_425794 357 8 2014 2014 CD 10_1101-2021_01_07_425794 357 9 . . . 10_1101-2021_01_07_425794 358 1 24 24 CD 10_1101-2021_01_07_425794 358 2 .CC .CC : 10_1101-2021_01_07_425794 358 3 - - HYPH 10_1101-2021_01_07_425794 358 4 BY by IN 10_1101-2021_01_07_425794 358 5 - - HYPH 10_1101-2021_01_07_425794 358 6 NC NC NNP 10_1101-2021_01_07_425794 358 7 - - HYPH 10_1101-2021_01_07_425794 358 8 ND ND NNP 10_1101-2021_01_07_425794 358 9 4.0 4.0 CD 10_1101-2021_01_07_425794 358 10 International international JJ 10_1101-2021_01_07_425794 358 11 licensemade licensemade NN 10_1101-2021_01_07_425794 358 12 available available JJ 10_1101-2021_01_07_425794 358 13 under under IN 10_1101-2021_01_07_425794 358 14 a a DT 10_1101-2021_01_07_425794 358 15 ( ( -LRB- 10_1101-2021_01_07_425794 358 16 which which WDT 10_1101-2021_01_07_425794 358 17 was be VBD 10_1101-2021_01_07_425794 358 18 not not RB 10_1101-2021_01_07_425794 358 19 certified certify VBN 10_1101-2021_01_07_425794 358 20 by by IN 10_1101-2021_01_07_425794 358 21 peer peer NN 10_1101-2021_01_07_425794 358 22 review review NN 10_1101-2021_01_07_425794 358 23 ) ) -RRB- 10_1101-2021_01_07_425794 358 24 is be VBZ 10_1101-2021_01_07_425794 358 25 the the DT 10_1101-2021_01_07_425794 358 26 author author NN 10_1101-2021_01_07_425794 358 27 / / SYM 10_1101-2021_01_07_425794 358 28 funder funder NN 10_1101-2021_01_07_425794 358 29 , , , 10_1101-2021_01_07_425794 358 30 who who WP 10_1101-2021_01_07_425794 358 31 has have VBZ 10_1101-2021_01_07_425794 358 32 granted grant VBN 10_1101-2021_01_07_425794 358 33 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 358 34 a a DT 10_1101-2021_01_07_425794 358 35 license license NN 10_1101-2021_01_07_425794 358 36 to to TO 10_1101-2021_01_07_425794 358 37 display display VB 10_1101-2021_01_07_425794 358 38 the the DT 10_1101-2021_01_07_425794 358 39 preprint preprint NN 10_1101-2021_01_07_425794 358 40 in in IN 10_1101-2021_01_07_425794 358 41 perpetuity perpetuity NN 10_1101-2021_01_07_425794 358 42 . . . 10_1101-2021_01_07_425794 359 1 It -PRON- PRP 10_1101-2021_01_07_425794 359 2 is be VBZ 10_1101-2021_01_07_425794 359 3 The the DT 10_1101-2021_01_07_425794 359 4 copyright copyright NN 10_1101-2021_01_07_425794 359 5 holder holder NN 10_1101-2021_01_07_425794 359 6 for for IN 10_1101-2021_01_07_425794 359 7 this this DT 10_1101-2021_01_07_425794 359 8 preprintthis preprintthis NN 10_1101-2021_01_07_425794 359 9 version version NN 10_1101-2021_01_07_425794 359 10 posted post VBD 10_1101-2021_01_07_425794 359 11 January January NNP 10_1101-2021_01_07_425794 359 12 8 8 CD 10_1101-2021_01_07_425794 359 13 , , , 10_1101-2021_01_07_425794 359 14 2021 2021 CD 10_1101-2021_01_07_425794 359 15 . . . 10_1101-2021_01_07_425794 359 16 ; ; : 10_1101-2021_01_07_425794 359 17 https://doi.org/10.1101/2021.01.07.425794doi https://doi.org/10.1101/2021.01.07.425794doi ADD 10_1101-2021_01_07_425794 359 18 : : : 10_1101-2021_01_07_425794 359 19 bioRxiv biorxiv IN 10_1101-2021_01_07_425794 359 20 preprint preprint JJ 10_1101-2021_01_07_425794 359 21 https://doi.org/10.1101/2021.01.07.425794 https://doi.org/10.1101/2021.01.07.425794 NN 10_1101-2021_01_07_425794 359 22 http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ NN