id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
10_1101-2020_12_26_424429	Nayar, Gowri	Analysis and Forecasting of Global RT-PCR Primers for SARS-CoV-2	2021	13	.pdf	application/pdf	4921	407	61	SARS-CoV-2 primers in use today by measuring the number of mismatches between primer sequence and genome targets with respect to the sequenced SARS-CoV-2 genomes, we can measure how the targeted proteins are mutating. primer sequences and protocols developed for six different regions – USA, Germany, China, Hong Kong, Japan, and Thailand – percent of genomes hit by each PCR test, labelled by the country and target gene region. Figure 6 shows the average number of mismatches over time, grouped by the genomes sampled The results of this study also demonstrate that each primer target develops a different number of mismatches over time The mutations that lead to mismatches between gene PCR primers and their targets reflect the sequence evolution of the consistent with the observed increasing number of mismatches over time, and shows that evolution of SARS-CoV-2 genomes is of the RdRp gene, primer target creates a disproportionate number of mismatches when compared to genomes sequenced within	./cache/10_1101-2020_12_26_424429.pdf	./txt/10_1101-2020_12_26_424429.txt