id sid tid token lemma pos 10_1101-2021_01_08_425864 1 1 Fatty fatty NN 10_1101-2021_01_08_425864 1 2 acid acid NN 10_1101-2021_01_08_425864 1 3 oxidation oxidation NN 10_1101-2021_01_08_425864 1 4 participates participate NNS 10_1101-2021_01_08_425864 1 5 of of IN 10_1101-2021_01_08_425864 1 6 the the DT 10_1101-2021_01_08_425864 1 7 survival survival NN 10_1101-2021_01_08_425864 1 8 to to IN 10_1101-2021_01_08_425864 1 9 starvation starvation NN 10_1101-2021_01_08_425864 1 10 , , , 10_1101-2021_01_08_425864 1 11 cell cell NN 10_1101-2021_01_08_425864 1 12 cycle cycle NN 10_1101-2021_01_08_425864 1 13 progression progression NN 10_1101-2021_01_08_425864 1 14 and and CC 10_1101-2021_01_08_425864 1 15 differentiation differentiation NN 10_1101-2021_01_08_425864 1 16 in in IN 10_1101-2021_01_08_425864 1 17 the the DT 10_1101-2021_01_08_425864 1 18 insect insect JJ 10_1101-2021_01_08_425864 1 19 stages stage NNS 10_1101-2021_01_08_425864 1 20 of of IN 10_1101-2021_01_08_425864 1 21 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 1 22 cruzi cruzi NNS 10_1101-2021_01_08_425864 1 23 1 1 CD 10_1101-2021_01_08_425864 1 24 2 2 CD 10_1101-2021_01_08_425864 1 25 3 3 CD 10_1101-2021_01_08_425864 1 26 4 4 CD 10_1101-2021_01_08_425864 1 27 Fatty Fatty NNP 10_1101-2021_01_08_425864 1 28 acid acid NN 10_1101-2021_01_08_425864 1 29 oxidation oxidation NN 10_1101-2021_01_08_425864 1 30 participates participate NNS 10_1101-2021_01_08_425864 1 31 of of IN 10_1101-2021_01_08_425864 1 32 the the DT 10_1101-2021_01_08_425864 1 33 survival survival NN 10_1101-2021_01_08_425864 1 34 to to IN 10_1101-2021_01_08_425864 1 35 starvation starvation NN 10_1101-2021_01_08_425864 1 36 , , , 10_1101-2021_01_08_425864 1 37 cell cell NN 10_1101-2021_01_08_425864 1 38 cycle cycle NN 10_1101-2021_01_08_425864 1 39 progression progression NN 10_1101-2021_01_08_425864 1 40 5 5 CD 10_1101-2021_01_08_425864 1 41 and and CC 10_1101-2021_01_08_425864 1 42 differentiation differentiation NN 10_1101-2021_01_08_425864 1 43 in in IN 10_1101-2021_01_08_425864 1 44 the the DT 10_1101-2021_01_08_425864 1 45 insect insect JJ 10_1101-2021_01_08_425864 1 46 stages stage NNS 10_1101-2021_01_08_425864 1 47 of of IN 10_1101-2021_01_08_425864 1 48 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 1 49 cruzi cruzi NNS 10_1101-2021_01_08_425864 1 50 6 6 CD 10_1101-2021_01_08_425864 1 51 7 7 CD 10_1101-2021_01_08_425864 1 52 Rodolpho Rodolpho NNP 10_1101-2021_01_08_425864 1 53 Ornitz Ornitz NNP 10_1101-2021_01_08_425864 1 54 Oliveira Oliveira NNP 10_1101-2021_01_08_425864 1 55 Souza¹ Souza¹ NNP 10_1101-2021_01_08_425864 1 56 , , , 10_1101-2021_01_08_425864 1 57 Flávia Flávia NNP 10_1101-2021_01_08_425864 1 58 Silva Silva NNP 10_1101-2021_01_08_425864 1 59 Damasceno¹ Damasceno¹ NNP 10_1101-2021_01_08_425864 1 60 , , , 10_1101-2021_01_08_425864 1 61 Sabrina Sabrina NNP 10_1101-2021_01_08_425864 1 62 Marsiccobetre1 Marsiccobetre1 NNP 10_1101-2021_01_08_425864 1 63 , , , 10_1101-2021_01_08_425864 1 64 Marc Marc NNP 10_1101-2021_01_08_425864 1 65 Biran2 Biran2 NNP 10_1101-2021_01_08_425864 1 66 , , , 10_1101-2021_01_08_425864 1 67 8 8 CD 10_1101-2021_01_08_425864 1 68 Gilson Gilson NNP 10_1101-2021_01_08_425864 1 69 Murata3 Murata3 NNP 10_1101-2021_01_08_425864 1 70 , , , 10_1101-2021_01_08_425864 1 71 Rui Rui NNP 10_1101-2021_01_08_425864 1 72 Curi4 curi4 CD 10_1101-2021_01_08_425864 1 73 , , , 10_1101-2021_01_08_425864 1 74 Frédéric Frédéric NNP 10_1101-2021_01_08_425864 1 75 Bringaud5 Bringaud5 NNP 10_1101-2021_01_08_425864 1 76 , , , 10_1101-2021_01_08_425864 1 77 Ariel Ariel NNP 10_1101-2021_01_08_425864 1 78 Mariano Mariano NNP 10_1101-2021_01_08_425864 1 79 Silber¹ Silber¹ NNP 10_1101-2021_01_08_425864 1 80 * * NFP 10_1101-2021_01_08_425864 1 81 9 9 CD 10_1101-2021_01_08_425864 1 82 10 10 CD 10_1101-2021_01_08_425864 1 83 11 11 CD 10_1101-2021_01_08_425864 1 84 ¹ ¹ CD 10_1101-2021_01_08_425864 1 85 University University NNP 10_1101-2021_01_08_425864 1 86 of of IN 10_1101-2021_01_08_425864 1 87 São São NNP 10_1101-2021_01_08_425864 1 88 Paulo Paulo NNP 10_1101-2021_01_08_425864 1 89 , , , 10_1101-2021_01_08_425864 1 90 Laboratory Laboratory NNP 10_1101-2021_01_08_425864 1 91 of of IN 10_1101-2021_01_08_425864 1 92 Biochemistry Biochemistry NNP 10_1101-2021_01_08_425864 1 93 of of IN 10_1101-2021_01_08_425864 1 94 Tryps Tryps NNP 10_1101-2021_01_08_425864 1 95 – – : 10_1101-2021_01_08_425864 1 96 LaBTryps LaBTryps NNP 10_1101-2021_01_08_425864 1 97 , , , 10_1101-2021_01_08_425864 1 98 Department Department NNP 10_1101-2021_01_08_425864 1 99 of of IN 10_1101-2021_01_08_425864 1 100 12 12 CD 10_1101-2021_01_08_425864 1 101 Parasitology Parasitology NNP 10_1101-2021_01_08_425864 1 102 , , , 10_1101-2021_01_08_425864 1 103 Institute Institute NNP 10_1101-2021_01_08_425864 1 104 of of IN 10_1101-2021_01_08_425864 1 105 Biomedical Biomedical NNP 10_1101-2021_01_08_425864 1 106 Sciences Sciences NNPS 10_1101-2021_01_08_425864 1 107 – – : 10_1101-2021_01_08_425864 1 108 São São NNP 10_1101-2021_01_08_425864 1 109 Paulo Paulo NNP 10_1101-2021_01_08_425864 1 110 , , , 10_1101-2021_01_08_425864 1 111 SP SP NNP 10_1101-2021_01_08_425864 1 112 , , , 10_1101-2021_01_08_425864 1 113 Brazil Brazil NNP 10_1101-2021_01_08_425864 1 114 13 13 CD 10_1101-2021_01_08_425864 1 115 14 14 CD 10_1101-2021_01_08_425864 1 116 2 2 CD 10_1101-2021_01_08_425864 1 117 Centre Centre NNP 10_1101-2021_01_08_425864 1 118 de de IN 10_1101-2021_01_08_425864 1 119 Résonance Résonance NNP 10_1101-2021_01_08_425864 1 120 Magnétique Magnétique NNP 10_1101-2021_01_08_425864 1 121 des des NNP 10_1101-2021_01_08_425864 1 122 Systèmes Systèmes NNP 10_1101-2021_01_08_425864 1 123 Biologiques Biologiques NNP 10_1101-2021_01_08_425864 1 124 ( ( -LRB- 10_1101-2021_01_08_425864 1 125 RMSB RMSB NNP 10_1101-2021_01_08_425864 1 126 ) ) -RRB- 10_1101-2021_01_08_425864 1 127 , , , 10_1101-2021_01_08_425864 1 128 Université Université NNP 10_1101-2021_01_08_425864 1 129 de de NNP 10_1101-2021_01_08_425864 1 130 Bordeaux Bordeaux NNP 10_1101-2021_01_08_425864 1 131 , , , 10_1101-2021_01_08_425864 1 132 15 15 CD 10_1101-2021_01_08_425864 1 133 CNRS CNRS NNP 10_1101-2021_01_08_425864 1 134 UMR-5536 UMR-5536 NNP 10_1101-2021_01_08_425864 1 135 , , , 10_1101-2021_01_08_425864 1 136 Bordeaux Bordeaux NNP 10_1101-2021_01_08_425864 1 137 , , , 10_1101-2021_01_08_425864 1 138 France France NNP 10_1101-2021_01_08_425864 1 139 16 16 CD 10_1101-2021_01_08_425864 1 140 17 17 CD 10_1101-2021_01_08_425864 1 141 3 3 CD 10_1101-2021_01_08_425864 1 142 University University NNP 10_1101-2021_01_08_425864 1 143 of of IN 10_1101-2021_01_08_425864 1 144 São São NNP 10_1101-2021_01_08_425864 1 145 Paulo Paulo NNP 10_1101-2021_01_08_425864 1 146 , , , 10_1101-2021_01_08_425864 1 147 Department Department NNP 10_1101-2021_01_08_425864 1 148 of of IN 10_1101-2021_01_08_425864 1 149 Physiology Physiology NNP 10_1101-2021_01_08_425864 1 150 , , , 10_1101-2021_01_08_425864 1 151 Institute Institute NNP 10_1101-2021_01_08_425864 1 152 of of IN 10_1101-2021_01_08_425864 1 153 Biomedical Biomedical NNP 10_1101-2021_01_08_425864 1 154 Sciences Sciences NNPS 10_1101-2021_01_08_425864 1 155 – – : 10_1101-2021_01_08_425864 1 156 São São NNP 10_1101-2021_01_08_425864 1 157 Paulo Paulo NNP 10_1101-2021_01_08_425864 1 158 , , , 10_1101-2021_01_08_425864 1 159 18 18 CD 10_1101-2021_01_08_425864 1 160 SP SP NNP 10_1101-2021_01_08_425864 1 161 , , , 10_1101-2021_01_08_425864 1 162 Brazil Brazil NNP 10_1101-2021_01_08_425864 1 163 19 19 CD 10_1101-2021_01_08_425864 1 164 20 20 CD 10_1101-2021_01_08_425864 1 165 4 4 CD 10_1101-2021_01_08_425864 1 166 Cruzeiro Cruzeiro NNP 10_1101-2021_01_08_425864 1 167 do do VBP 10_1101-2021_01_08_425864 1 168 Sul Sul NNP 10_1101-2021_01_08_425864 1 169 University University NNP 10_1101-2021_01_08_425864 1 170 , , , 10_1101-2021_01_08_425864 1 171 Interdisciplinary Interdisciplinary NNP 10_1101-2021_01_08_425864 1 172 Post Post NNP 10_1101-2021_01_08_425864 1 173 - - NNP 10_1101-2021_01_08_425864 1 174 Graduate Graduate NNP 10_1101-2021_01_08_425864 1 175 Program Program NNP 10_1101-2021_01_08_425864 1 176 in in IN 10_1101-2021_01_08_425864 1 177 Health Health NNP 10_1101-2021_01_08_425864 1 178 Sciences Sciences NNPS 10_1101-2021_01_08_425864 1 179 - - HYPH 10_1101-2021_01_08_425864 1 180 São São NNP 10_1101-2021_01_08_425864 1 181 Paulo Paulo NNP 10_1101-2021_01_08_425864 1 182 , , , 10_1101-2021_01_08_425864 1 183 21 21 CD 10_1101-2021_01_08_425864 1 184 SP SP NNP 10_1101-2021_01_08_425864 1 185 , , , 10_1101-2021_01_08_425864 1 186 Brazil Brazil NNP 10_1101-2021_01_08_425864 1 187 22 22 CD 10_1101-2021_01_08_425864 1 188 23 23 CD 10_1101-2021_01_08_425864 1 189 5 5 CD 10_1101-2021_01_08_425864 1 190 Laboratoire Laboratoire NNP 10_1101-2021_01_08_425864 1 191 de de IN 10_1101-2021_01_08_425864 1 192 Microbiologie Microbiologie NNP 10_1101-2021_01_08_425864 1 193 Fondamentale Fondamentale NNP 10_1101-2021_01_08_425864 1 194 et et NNP 10_1101-2021_01_08_425864 1 195 Pathogénicité Pathogénicité NNP 10_1101-2021_01_08_425864 1 196 ( ( -LRB- 10_1101-2021_01_08_425864 1 197 MFP MFP NNP 10_1101-2021_01_08_425864 1 198 ) ) -RRB- 10_1101-2021_01_08_425864 1 199 , , , 10_1101-2021_01_08_425864 1 200 Université Université NNP 10_1101-2021_01_08_425864 1 201 de de NNP 10_1101-2021_01_08_425864 1 202 Bordeaux Bordeaux NNP 10_1101-2021_01_08_425864 1 203 , , , 10_1101-2021_01_08_425864 1 204 24 24 CD 10_1101-2021_01_08_425864 1 205 CNRS cnr NNS 10_1101-2021_01_08_425864 1 206 UMR-5234 UMR-5234 NNP 10_1101-2021_01_08_425864 1 207 , , , 10_1101-2021_01_08_425864 1 208 Bordeaux Bordeaux NNP 10_1101-2021_01_08_425864 1 209 , , , 10_1101-2021_01_08_425864 1 210 France France NNP 10_1101-2021_01_08_425864 1 211 25 25 CD 10_1101-2021_01_08_425864 1 212 26 26 CD 10_1101-2021_01_08_425864 1 213 27 27 CD 10_1101-2021_01_08_425864 1 214 28 28 CD 10_1101-2021_01_08_425864 1 215 * * NFP 10_1101-2021_01_08_425864 1 216 Corresponding correspond VBG 10_1101-2021_01_08_425864 1 217 author author NN 10_1101-2021_01_08_425864 1 218 29 29 CD 10_1101-2021_01_08_425864 1 219 E e NN 10_1101-2021_01_08_425864 1 220 - - NN 10_1101-2021_01_08_425864 1 221 mail mail NN 10_1101-2021_01_08_425864 1 222 : : : 10_1101-2021_01_08_425864 1 223 asilber@usp.br asilber@usp.br NNP 10_1101-2021_01_08_425864 1 224 ( ( -LRB- 10_1101-2021_01_08_425864 1 225 AMS AMS NNP 10_1101-2021_01_08_425864 1 226 ) ) -RRB- 10_1101-2021_01_08_425864 1 227 30 30 CD 10_1101-2021_01_08_425864 1 228 31 31 CD 10_1101-2021_01_08_425864 1 229 32 32 CD 10_1101-2021_01_08_425864 1 230 33 33 CD 10_1101-2021_01_08_425864 1 231 .CC .cc CD 10_1101-2021_01_08_425864 1 232 - - : 10_1101-2021_01_08_425864 1 233 BY by IN 10_1101-2021_01_08_425864 1 234 4.0 4.0 CD 10_1101-2021_01_08_425864 1 235 International international JJ 10_1101-2021_01_08_425864 1 236 licensemade licensemade NN 10_1101-2021_01_08_425864 1 237 available available JJ 10_1101-2021_01_08_425864 1 238 under under IN 10_1101-2021_01_08_425864 1 239 a a DT 10_1101-2021_01_08_425864 1 240 ( ( -LRB- 10_1101-2021_01_08_425864 1 241 which which WDT 10_1101-2021_01_08_425864 1 242 was be VBD 10_1101-2021_01_08_425864 1 243 not not RB 10_1101-2021_01_08_425864 1 244 certified certify VBN 10_1101-2021_01_08_425864 1 245 by by IN 10_1101-2021_01_08_425864 1 246 peer peer NN 10_1101-2021_01_08_425864 1 247 review review NN 10_1101-2021_01_08_425864 1 248 ) ) -RRB- 10_1101-2021_01_08_425864 1 249 is be VBZ 10_1101-2021_01_08_425864 1 250 the the DT 10_1101-2021_01_08_425864 1 251 author author NN 10_1101-2021_01_08_425864 1 252 / / SYM 10_1101-2021_01_08_425864 1 253 funder funder NN 10_1101-2021_01_08_425864 1 254 , , , 10_1101-2021_01_08_425864 1 255 who who WP 10_1101-2021_01_08_425864 1 256 has have VBZ 10_1101-2021_01_08_425864 1 257 granted grant VBN 10_1101-2021_01_08_425864 1 258 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 1 259 a a DT 10_1101-2021_01_08_425864 1 260 license license NN 10_1101-2021_01_08_425864 1 261 to to TO 10_1101-2021_01_08_425864 1 262 display display VB 10_1101-2021_01_08_425864 1 263 the the DT 10_1101-2021_01_08_425864 1 264 preprint preprint NN 10_1101-2021_01_08_425864 1 265 in in IN 10_1101-2021_01_08_425864 1 266 perpetuity perpetuity NN 10_1101-2021_01_08_425864 1 267 . . . 10_1101-2021_01_08_425864 2 1 It -PRON- PRP 10_1101-2021_01_08_425864 2 2 is be VBZ 10_1101-2021_01_08_425864 2 3 The the DT 10_1101-2021_01_08_425864 2 4 copyright copyright NN 10_1101-2021_01_08_425864 2 5 holder holder NN 10_1101-2021_01_08_425864 2 6 for for IN 10_1101-2021_01_08_425864 2 7 this this DT 10_1101-2021_01_08_425864 2 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 2 9 version version NN 10_1101-2021_01_08_425864 2 10 posted post VBD 10_1101-2021_01_08_425864 2 11 January January NNP 10_1101-2021_01_08_425864 2 12 8 8 CD 10_1101-2021_01_08_425864 2 13 , , , 10_1101-2021_01_08_425864 2 14 2021 2021 CD 10_1101-2021_01_08_425864 2 15 . . . 10_1101-2021_01_08_425864 2 16 ; ; : 10_1101-2021_01_08_425864 2 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 2 18 : : : 10_1101-2021_01_08_425864 2 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 2 20 preprint preprint NN 10_1101-2021_01_08_425864 2 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 2 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 2 23 34 34 CD 10_1101-2021_01_08_425864 2 24 Abstract Abstract NNP 10_1101-2021_01_08_425864 2 25 35 35 CD 10_1101-2021_01_08_425864 2 26 During during IN 10_1101-2021_01_08_425864 2 27 its -PRON- PRP$ 10_1101-2021_01_08_425864 2 28 complex complex JJ 10_1101-2021_01_08_425864 2 29 life life NN 10_1101-2021_01_08_425864 2 30 cycle cycle NN 10_1101-2021_01_08_425864 2 31 , , , 10_1101-2021_01_08_425864 2 32 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 2 33 cruzi cruzi NNS 10_1101-2021_01_08_425864 2 34 colonizes colonize VBZ 10_1101-2021_01_08_425864 2 35 different different JJ 10_1101-2021_01_08_425864 2 36 niches niche NNS 10_1101-2021_01_08_425864 2 37 in in IN 10_1101-2021_01_08_425864 2 38 its -PRON- PRP$ 10_1101-2021_01_08_425864 2 39 insect insect NN 10_1101-2021_01_08_425864 2 40 and and CC 10_1101-2021_01_08_425864 2 41 36 36 CD 10_1101-2021_01_08_425864 2 42 mammalian mammalian JJ 10_1101-2021_01_08_425864 2 43 hosts host NNS 10_1101-2021_01_08_425864 2 44 . . . 10_1101-2021_01_08_425864 3 1 This this DT 10_1101-2021_01_08_425864 3 2 characteristic characteristic NN 10_1101-2021_01_08_425864 3 3 determined determine VBD 10_1101-2021_01_08_425864 3 4 the the DT 10_1101-2021_01_08_425864 3 5 types type NNS 10_1101-2021_01_08_425864 3 6 of of IN 10_1101-2021_01_08_425864 3 7 parasites parasite NNS 10_1101-2021_01_08_425864 3 8 that that WDT 10_1101-2021_01_08_425864 3 9 adapted adapt VBD 10_1101-2021_01_08_425864 3 10 to to TO 10_1101-2021_01_08_425864 3 11 face face VB 10_1101-2021_01_08_425864 3 12 37 37 CD 10_1101-2021_01_08_425864 3 13 challenging challenge VBG 10_1101-2021_01_08_425864 3 14 environmental environmental JJ 10_1101-2021_01_08_425864 3 15 cues cue NNS 10_1101-2021_01_08_425864 3 16 . . . 10_1101-2021_01_08_425864 4 1 The the DT 10_1101-2021_01_08_425864 4 2 primary primary JJ 10_1101-2021_01_08_425864 4 3 environmental environmental JJ 10_1101-2021_01_08_425864 4 4 challenge challenge NN 10_1101-2021_01_08_425864 4 5 , , , 10_1101-2021_01_08_425864 4 6 particularly particularly RB 10_1101-2021_01_08_425864 4 7 in in IN 10_1101-2021_01_08_425864 4 8 the the DT 10_1101-2021_01_08_425864 4 9 insect insect NN 10_1101-2021_01_08_425864 4 10 38 38 CD 10_1101-2021_01_08_425864 4 11 stages stage NNS 10_1101-2021_01_08_425864 4 12 , , , 10_1101-2021_01_08_425864 4 13 is be VBZ 10_1101-2021_01_08_425864 4 14 poor poor JJ 10_1101-2021_01_08_425864 4 15 nutrient nutrient NN 10_1101-2021_01_08_425864 4 16 availability availability NN 10_1101-2021_01_08_425864 4 17 . . . 10_1101-2021_01_08_425864 5 1 These these DT 10_1101-2021_01_08_425864 5 2 T. t. NN 10_1101-2021_01_08_425864 5 3 cruzi cruzi NNS 10_1101-2021_01_08_425864 5 4 stages stage NNS 10_1101-2021_01_08_425864 5 5 could could MD 10_1101-2021_01_08_425864 5 6 be be VB 10_1101-2021_01_08_425864 5 7 exposed expose VBN 10_1101-2021_01_08_425864 5 8 to to IN 10_1101-2021_01_08_425864 5 9 fatty fatty NN 10_1101-2021_01_08_425864 5 10 acids acid NNS 10_1101-2021_01_08_425864 5 11 originating originate VBG 10_1101-2021_01_08_425864 5 12 39 39 CD 10_1101-2021_01_08_425864 5 13 from from IN 10_1101-2021_01_08_425864 5 14 the the DT 10_1101-2021_01_08_425864 5 15 degradation degradation NN 10_1101-2021_01_08_425864 5 16 of of IN 10_1101-2021_01_08_425864 5 17 the the DT 10_1101-2021_01_08_425864 5 18 perimicrovillar perimicrovillar JJ 10_1101-2021_01_08_425864 5 19 membrane membrane NN 10_1101-2021_01_08_425864 5 20 . . . 10_1101-2021_01_08_425864 6 1 In in IN 10_1101-2021_01_08_425864 6 2 this this DT 10_1101-2021_01_08_425864 6 3 study study NN 10_1101-2021_01_08_425864 6 4 , , , 10_1101-2021_01_08_425864 6 5 we -PRON- PRP 10_1101-2021_01_08_425864 6 6 revisit revisit VBP 10_1101-2021_01_08_425864 6 7 the the DT 10_1101-2021_01_08_425864 6 8 metabolic metabolic JJ 10_1101-2021_01_08_425864 6 9 fate fate NN 10_1101-2021_01_08_425864 6 10 of of IN 10_1101-2021_01_08_425864 6 11 40 40 CD 10_1101-2021_01_08_425864 6 12 fatty fatty NN 10_1101-2021_01_08_425864 6 13 acid acid NN 10_1101-2021_01_08_425864 6 14 breakdown breakdown NN 10_1101-2021_01_08_425864 6 15 in in IN 10_1101-2021_01_08_425864 6 16 T. T. NNP 10_1101-2021_01_08_425864 6 17 cruzi cruzi NNS 10_1101-2021_01_08_425864 6 18 . . . 10_1101-2021_01_08_425864 7 1 Herein Herein NNP 10_1101-2021_01_08_425864 7 2 , , , 10_1101-2021_01_08_425864 7 3 we -PRON- PRP 10_1101-2021_01_08_425864 7 4 show show VBP 10_1101-2021_01_08_425864 7 5 that that IN 10_1101-2021_01_08_425864 7 6 during during IN 10_1101-2021_01_08_425864 7 7 parasite parasite NN 10_1101-2021_01_08_425864 7 8 proliferation proliferation NNP 10_1101-2021_01_08_425864 7 9 , , , 10_1101-2021_01_08_425864 7 10 the the DT 10_1101-2021_01_08_425864 7 11 glucose glucose NN 10_1101-2021_01_08_425864 7 12 41 41 CD 10_1101-2021_01_08_425864 7 13 concentration concentration NN 10_1101-2021_01_08_425864 7 14 in in IN 10_1101-2021_01_08_425864 7 15 the the DT 10_1101-2021_01_08_425864 7 16 medium medium NN 10_1101-2021_01_08_425864 7 17 can can MD 10_1101-2021_01_08_425864 7 18 regulate regulate VB 10_1101-2021_01_08_425864 7 19 the the DT 10_1101-2021_01_08_425864 7 20 fatty fatty NN 10_1101-2021_01_08_425864 7 21 acid acid NN 10_1101-2021_01_08_425864 7 22 metabolism metabolism NN 10_1101-2021_01_08_425864 7 23 . . . 10_1101-2021_01_08_425864 8 1 At at IN 10_1101-2021_01_08_425864 8 2 the the DT 10_1101-2021_01_08_425864 8 3 stationary stationary JJ 10_1101-2021_01_08_425864 8 4 phase phase NN 10_1101-2021_01_08_425864 8 5 , , , 10_1101-2021_01_08_425864 8 6 the the DT 10_1101-2021_01_08_425864 8 7 42 42 CD 10_1101-2021_01_08_425864 8 8 parasites parasite NNS 10_1101-2021_01_08_425864 8 9 fully fully RB 10_1101-2021_01_08_425864 8 10 oxidize oxidize VBP 10_1101-2021_01_08_425864 8 11 fatty fatty NN 10_1101-2021_01_08_425864 8 12 acids acid NNS 10_1101-2021_01_08_425864 8 13 . . . 10_1101-2021_01_08_425864 9 1 [ [ -LRB- 10_1101-2021_01_08_425864 9 2 U-14C]-palmitate u-14c]-palmitate CD 10_1101-2021_01_08_425864 9 3 can can MD 10_1101-2021_01_08_425864 9 4 be be VB 10_1101-2021_01_08_425864 9 5 taken take VBN 10_1101-2021_01_08_425864 9 6 up up RP 10_1101-2021_01_08_425864 9 7 from from IN 10_1101-2021_01_08_425864 9 8 the the DT 10_1101-2021_01_08_425864 9 9 medium medium NN 10_1101-2021_01_08_425864 9 10 , , , 10_1101-2021_01_08_425864 9 11 leading lead VBG 10_1101-2021_01_08_425864 9 12 to to IN 10_1101-2021_01_08_425864 9 13 43 43 CD 10_1101-2021_01_08_425864 9 14 CO2 CO2 NNP 10_1101-2021_01_08_425864 9 15 production production NN 10_1101-2021_01_08_425864 9 16 via via IN 10_1101-2021_01_08_425864 9 17 beta beta NN 10_1101-2021_01_08_425864 9 18 - - HYPH 10_1101-2021_01_08_425864 9 19 oxidation oxidation NN 10_1101-2021_01_08_425864 9 20 . . . 10_1101-2021_01_08_425864 10 1 Lastly lastly RB 10_1101-2021_01_08_425864 10 2 , , , 10_1101-2021_01_08_425864 10 3 we -PRON- PRP 10_1101-2021_01_08_425864 10 4 also also RB 10_1101-2021_01_08_425864 10 5 show show VBP 10_1101-2021_01_08_425864 10 6 that that IN 10_1101-2021_01_08_425864 10 7 fatty fatty NN 10_1101-2021_01_08_425864 10 8 acids acid NNS 10_1101-2021_01_08_425864 10 9 are be VBP 10_1101-2021_01_08_425864 10 10 degraded degrade VBN 10_1101-2021_01_08_425864 10 11 through through IN 10_1101-2021_01_08_425864 10 12 beta- beta- JJ 10_1101-2021_01_08_425864 10 13 44 44 CD 10_1101-2021_01_08_425864 10 14 oxidation oxidation NN 10_1101-2021_01_08_425864 10 15 . . . 10_1101-2021_01_08_425864 11 1 Additionally additionally RB 10_1101-2021_01_08_425864 11 2 , , , 10_1101-2021_01_08_425864 11 3 through through IN 10_1101-2021_01_08_425864 11 4 beta beta NN 10_1101-2021_01_08_425864 11 5 - - HYPH 10_1101-2021_01_08_425864 11 6 oxidation oxidation NN 10_1101-2021_01_08_425864 11 7 , , , 10_1101-2021_01_08_425864 11 8 electrons electron NNS 10_1101-2021_01_08_425864 11 9 are be VBP 10_1101-2021_01_08_425864 11 10 fed feed VBN 10_1101-2021_01_08_425864 11 11 directly directly RB 10_1101-2021_01_08_425864 11 12 to to IN 10_1101-2021_01_08_425864 11 13 oxidative oxidative CD 10_1101-2021_01_08_425864 11 14 45 45 CD 10_1101-2021_01_08_425864 11 15 phosphorylation phosphorylation NN 10_1101-2021_01_08_425864 11 16 , , , 10_1101-2021_01_08_425864 11 17 and and CC 10_1101-2021_01_08_425864 11 18 acetyl acetyl NN 10_1101-2021_01_08_425864 11 19 - - HYPH 10_1101-2021_01_08_425864 11 20 CoA CoA NNP 10_1101-2021_01_08_425864 11 21 is be VBZ 10_1101-2021_01_08_425864 11 22 supplied supply VBN 10_1101-2021_01_08_425864 11 23 to to IN 10_1101-2021_01_08_425864 11 24 the the DT 10_1101-2021_01_08_425864 11 25 tricarboxylic tricarboxylic JJ 10_1101-2021_01_08_425864 11 26 acid acid NN 10_1101-2021_01_08_425864 11 27 cycle cycle NN 10_1101-2021_01_08_425864 11 28 , , , 10_1101-2021_01_08_425864 11 29 which which WDT 10_1101-2021_01_08_425864 11 30 can can MD 10_1101-2021_01_08_425864 11 31 be be VB 10_1101-2021_01_08_425864 11 32 used use VBN 10_1101-2021_01_08_425864 11 33 to to TO 10_1101-2021_01_08_425864 11 34 46 46 CD 10_1101-2021_01_08_425864 11 35 feed feed VB 10_1101-2021_01_08_425864 11 36 other other JJ 10_1101-2021_01_08_425864 11 37 anabolic anabolic JJ 10_1101-2021_01_08_425864 11 38 pathways pathway NNS 10_1101-2021_01_08_425864 11 39 such such JJ 10_1101-2021_01_08_425864 11 40 as as IN 10_1101-2021_01_08_425864 11 41 the the DT 10_1101-2021_01_08_425864 11 42 de de FW 10_1101-2021_01_08_425864 11 43 novo novo NNP 10_1101-2021_01_08_425864 11 44 biosynthesis biosynthesis NNP 10_1101-2021_01_08_425864 11 45 of of IN 10_1101-2021_01_08_425864 11 46 fatty fatty NNP 10_1101-2021_01_08_425864 11 47 acids acid NNS 10_1101-2021_01_08_425864 11 48 . . . 10_1101-2021_01_08_425864 12 1 47 47 CD 10_1101-2021_01_08_425864 12 2 48 48 CD 10_1101-2021_01_08_425864 12 3 Author author NN 10_1101-2021_01_08_425864 12 4 Summary Summary NNP 10_1101-2021_01_08_425864 12 5 49 49 CD 10_1101-2021_01_08_425864 12 6 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 12 7 cruzi cruzi NNS 10_1101-2021_01_08_425864 12 8 is be VBZ 10_1101-2021_01_08_425864 12 9 a a DT 10_1101-2021_01_08_425864 12 10 protist protist JJ 10_1101-2021_01_08_425864 12 11 parasite parasite NN 10_1101-2021_01_08_425864 12 12 with with IN 10_1101-2021_01_08_425864 12 13 a a DT 10_1101-2021_01_08_425864 12 14 life life NN 10_1101-2021_01_08_425864 12 15 cycle cycle NN 10_1101-2021_01_08_425864 12 16 involving involve VBG 10_1101-2021_01_08_425864 12 17 two two CD 10_1101-2021_01_08_425864 12 18 types type NNS 10_1101-2021_01_08_425864 12 19 of of IN 10_1101-2021_01_08_425864 12 20 hosts host NNS 10_1101-2021_01_08_425864 12 21 , , , 10_1101-2021_01_08_425864 12 22 a a DT 10_1101-2021_01_08_425864 12 23 50 50 CD 10_1101-2021_01_08_425864 12 24 vertebrate vertebrate NN 10_1101-2021_01_08_425864 12 25 one one CD 10_1101-2021_01_08_425864 12 26 ( ( -LRB- 10_1101-2021_01_08_425864 12 27 which which WDT 10_1101-2021_01_08_425864 12 28 includes include VBZ 10_1101-2021_01_08_425864 12 29 humans human NNS 10_1101-2021_01_08_425864 12 30 , , , 10_1101-2021_01_08_425864 12 31 causing cause VBG 10_1101-2021_01_08_425864 12 32 Chagas Chagas NNP 10_1101-2021_01_08_425864 12 33 disease disease NN 10_1101-2021_01_08_425864 12 34 ) ) -RRB- 10_1101-2021_01_08_425864 12 35 and and CC 10_1101-2021_01_08_425864 12 36 an an DT 10_1101-2021_01_08_425864 12 37 invertebrate invertebrate JJ 10_1101-2021_01_08_425864 12 38 one one NN 10_1101-2021_01_08_425864 12 39 ( ( -LRB- 10_1101-2021_01_08_425864 12 40 kissing kiss VBG 10_1101-2021_01_08_425864 12 41 51 51 CD 10_1101-2021_01_08_425864 12 42 bugs bug NNS 10_1101-2021_01_08_425864 12 43 , , , 10_1101-2021_01_08_425864 12 44 which which WDT 10_1101-2021_01_08_425864 12 45 vectorize vectorize VBP 10_1101-2021_01_08_425864 12 46 the the DT 10_1101-2021_01_08_425864 12 47 infection infection NN 10_1101-2021_01_08_425864 12 48 among among IN 10_1101-2021_01_08_425864 12 49 mammals mammal NNS 10_1101-2021_01_08_425864 12 50 ) ) -RRB- 10_1101-2021_01_08_425864 12 51 . . . 10_1101-2021_01_08_425864 13 1 In in IN 10_1101-2021_01_08_425864 13 2 both both DT 10_1101-2021_01_08_425864 13 3 hosts host NNS 10_1101-2021_01_08_425864 13 4 , , , 10_1101-2021_01_08_425864 13 5 the the DT 10_1101-2021_01_08_425864 13 6 parasite parasite NN 10_1101-2021_01_08_425864 13 7 faces face VBZ 10_1101-2021_01_08_425864 13 8 environmental environmental JJ 10_1101-2021_01_08_425864 13 9 52 52 CD 10_1101-2021_01_08_425864 13 10 challenges challenge NNS 10_1101-2021_01_08_425864 13 11 such such JJ 10_1101-2021_01_08_425864 13 12 as as IN 10_1101-2021_01_08_425864 13 13 sudden sudden JJ 10_1101-2021_01_08_425864 13 14 changes change NNS 10_1101-2021_01_08_425864 13 15 in in IN 10_1101-2021_01_08_425864 13 16 the the DT 10_1101-2021_01_08_425864 13 17 metabolic metabolic JJ 10_1101-2021_01_08_425864 13 18 composition composition NN 10_1101-2021_01_08_425864 13 19 of of IN 10_1101-2021_01_08_425864 13 20 the the DT 10_1101-2021_01_08_425864 13 21 medium medium NN 10_1101-2021_01_08_425864 13 22 in in IN 10_1101-2021_01_08_425864 13 23 which which WDT 10_1101-2021_01_08_425864 13 24 they -PRON- PRP 10_1101-2021_01_08_425864 13 25 53 53 CD 10_1101-2021_01_08_425864 13 26 develop develop VBP 10_1101-2021_01_08_425864 13 27 , , , 10_1101-2021_01_08_425864 13 28 severe severe JJ 10_1101-2021_01_08_425864 13 29 starvation starvation NN 10_1101-2021_01_08_425864 13 30 , , , 10_1101-2021_01_08_425864 13 31 osmotic osmotic JJ 10_1101-2021_01_08_425864 13 32 stress stress NN 10_1101-2021_01_08_425864 13 33 and and CC 10_1101-2021_01_08_425864 13 34 redox redox NN 10_1101-2021_01_08_425864 13 35 imbalance imbalance NN 10_1101-2021_01_08_425864 13 36 , , , 10_1101-2021_01_08_425864 13 37 among among IN 10_1101-2021_01_08_425864 13 38 others other NNS 10_1101-2021_01_08_425864 13 39 . . . 10_1101-2021_01_08_425864 14 1 Because because IN 10_1101-2021_01_08_425864 14 2 kissing kiss VBG 10_1101-2021_01_08_425864 14 3 bugs bug NNS 10_1101-2021_01_08_425864 14 4 54 54 CD 10_1101-2021_01_08_425864 14 5 feed feed NN 10_1101-2021_01_08_425864 14 6 infrequently infrequently RB 10_1101-2021_01_08_425864 14 7 in in IN 10_1101-2021_01_08_425864 14 8 nature nature NN 10_1101-2021_01_08_425864 14 9 , , , 10_1101-2021_01_08_425864 14 10 an an DT 10_1101-2021_01_08_425864 14 11 intriguing intriguing JJ 10_1101-2021_01_08_425864 14 12 aspect aspect NN 10_1101-2021_01_08_425864 14 13 of of IN 10_1101-2021_01_08_425864 14 14 T. T. NNP 10_1101-2021_01_08_425864 14 15 cruzi cruzi NNP 10_1101-2021_01_08_425864 14 16 biology biology NN 10_1101-2021_01_08_425864 14 17 ( ( -LRB- 10_1101-2021_01_08_425864 14 18 it -PRON- PRP 10_1101-2021_01_08_425864 14 19 exclusively exclusively RB 10_1101-2021_01_08_425864 14 20 inhabits inhabit VBZ 10_1101-2021_01_08_425864 14 21 the the DT 10_1101-2021_01_08_425864 14 22 55 55 CD 10_1101-2021_01_08_425864 14 23 digestive digestive JJ 10_1101-2021_01_08_425864 14 24 tube tube NN 10_1101-2021_01_08_425864 14 25 of of IN 10_1101-2021_01_08_425864 14 26 these these DT 10_1101-2021_01_08_425864 14 27 insects insect NNS 10_1101-2021_01_08_425864 14 28 ) ) -RRB- 10_1101-2021_01_08_425864 14 29 is be VBZ 10_1101-2021_01_08_425864 14 30 how how WRB 10_1101-2021_01_08_425864 14 31 they -PRON- PRP 10_1101-2021_01_08_425864 14 32 subsist subsist VBP 10_1101-2021_01_08_425864 14 33 during during IN 10_1101-2021_01_08_425864 14 34 long long JJ 10_1101-2021_01_08_425864 14 35 periods period NNS 10_1101-2021_01_08_425864 14 36 of of IN 10_1101-2021_01_08_425864 14 37 starvation starvation NN 10_1101-2021_01_08_425864 14 38 . . . 10_1101-2021_01_08_425864 15 1 In in IN 10_1101-2021_01_08_425864 15 2 this this DT 10_1101-2021_01_08_425864 15 3 work work NN 10_1101-2021_01_08_425864 15 4 , , , 10_1101-2021_01_08_425864 15 5 we -PRON- PRP 10_1101-2021_01_08_425864 15 6 56 56 CD 10_1101-2021_01_08_425864 15 7 show show VBP 10_1101-2021_01_08_425864 15 8 that that IN 10_1101-2021_01_08_425864 15 9 this this DT 10_1101-2021_01_08_425864 15 10 parasite parasite NN 10_1101-2021_01_08_425864 15 11 performs perform VBZ 10_1101-2021_01_08_425864 15 12 a a DT 10_1101-2021_01_08_425864 15 13 metabolic metabolic JJ 10_1101-2021_01_08_425864 15 14 switch switch NN 10_1101-2021_01_08_425864 15 15 from from IN 10_1101-2021_01_08_425864 15 16 glucose glucose VB 10_1101-2021_01_08_425864 15 17 consumption consumption NN 10_1101-2021_01_08_425864 15 18 to to IN 10_1101-2021_01_08_425864 15 19 lipid lipid NN 10_1101-2021_01_08_425864 15 20 oxidation oxidation NN 10_1101-2021_01_08_425864 15 21 , , , 10_1101-2021_01_08_425864 15 22 and and CC 10_1101-2021_01_08_425864 15 23 57 57 CD 10_1101-2021_01_08_425864 15 24 it -PRON- PRP 10_1101-2021_01_08_425864 15 25 is be VBZ 10_1101-2021_01_08_425864 15 26 able able JJ 10_1101-2021_01_08_425864 15 27 to to TO 10_1101-2021_01_08_425864 15 28 consume consume VB 10_1101-2021_01_08_425864 15 29 lipids lipid NNS 10_1101-2021_01_08_425864 15 30 and and CC 10_1101-2021_01_08_425864 15 31 the the DT 10_1101-2021_01_08_425864 15 32 lipid lipid NN 10_1101-2021_01_08_425864 15 33 - - HYPH 10_1101-2021_01_08_425864 15 34 derived derive VBN 10_1101-2021_01_08_425864 15 35 fatty fatty NN 10_1101-2021_01_08_425864 15 36 acids acid NNS 10_1101-2021_01_08_425864 15 37 from from IN 10_1101-2021_01_08_425864 15 38 both both DT 10_1101-2021_01_08_425864 15 39 internal internal JJ 10_1101-2021_01_08_425864 15 40 origins origin NNS 10_1101-2021_01_08_425864 15 41 as as RB 10_1101-2021_01_08_425864 15 42 well well RB 10_1101-2021_01_08_425864 15 43 as as IN 10_1101-2021_01_08_425864 15 44 58 58 CD 10_1101-2021_01_08_425864 15 45 externally externally RB 10_1101-2021_01_08_425864 15 46 supplied supply VBN 10_1101-2021_01_08_425864 15 47 compounds compound NNS 10_1101-2021_01_08_425864 15 48 . . . 10_1101-2021_01_08_425864 16 1 When when WRB 10_1101-2021_01_08_425864 16 2 fatty fatty NN 10_1101-2021_01_08_425864 16 3 acid acid NN 10_1101-2021_01_08_425864 16 4 oxidation oxidation NN 10_1101-2021_01_08_425864 16 5 is be VBZ 10_1101-2021_01_08_425864 16 6 chemically chemically RB 10_1101-2021_01_08_425864 16 7 inhibited inhibit VBN 10_1101-2021_01_08_425864 16 8 by by IN 10_1101-2021_01_08_425864 16 9 etomoxir etomoxir NNS 10_1101-2021_01_08_425864 16 10 , , , 10_1101-2021_01_08_425864 16 11 a a DT 10_1101-2021_01_08_425864 16 12 very very RB 10_1101-2021_01_08_425864 16 13 59 59 CD 10_1101-2021_01_08_425864 16 14 well well RB 10_1101-2021_01_08_425864 16 15 - - HYPH 10_1101-2021_01_08_425864 16 16 known know VBN 10_1101-2021_01_08_425864 16 17 drug drug NN 10_1101-2021_01_08_425864 16 18 that that WDT 10_1101-2021_01_08_425864 16 19 inhibits inhibit VBZ 10_1101-2021_01_08_425864 16 20 the the DT 10_1101-2021_01_08_425864 16 21 translocation translocation NN 10_1101-2021_01_08_425864 16 22 of of IN 10_1101-2021_01_08_425864 16 23 fatty fatty NN 10_1101-2021_01_08_425864 16 24 acids acid NNS 10_1101-2021_01_08_425864 16 25 into into IN 10_1101-2021_01_08_425864 16 26 the the DT 10_1101-2021_01_08_425864 16 27 mitochondria mitochondria NN 10_1101-2021_01_08_425864 16 28 , , , 10_1101-2021_01_08_425864 16 29 the the DT 10_1101-2021_01_08_425864 16 30 proliferative proliferative JJ 10_1101-2021_01_08_425864 16 31 60 60 CD 10_1101-2021_01_08_425864 16 32 insect insect JJ 10_1101-2021_01_08_425864 16 33 stage stage NN 10_1101-2021_01_08_425864 16 34 of of IN 10_1101-2021_01_08_425864 16 35 the the DT 10_1101-2021_01_08_425864 16 36 parasites parasite NNS 10_1101-2021_01_08_425864 16 37 has have VBZ 10_1101-2021_01_08_425864 16 38 dramatically dramatically RB 10_1101-2021_01_08_425864 16 39 diminished diminish VBN 10_1101-2021_01_08_425864 16 40 survival survival NN 10_1101-2021_01_08_425864 16 41 under under IN 10_1101-2021_01_08_425864 16 42 severe severe JJ 10_1101-2021_01_08_425864 16 43 metabolic metabolic JJ 10_1101-2021_01_08_425864 16 44 stress stress NN 10_1101-2021_01_08_425864 16 45 and and CC 10_1101-2021_01_08_425864 16 46 61 61 CD 10_1101-2021_01_08_425864 16 47 its -PRON- PRP$ 10_1101-2021_01_08_425864 16 48 differentiation differentiation NN 10_1101-2021_01_08_425864 16 49 into into IN 10_1101-2021_01_08_425864 16 50 its -PRON- PRP$ 10_1101-2021_01_08_425864 16 51 infective infective JJ 10_1101-2021_01_08_425864 16 52 forms form NNS 10_1101-2021_01_08_425864 16 53 is be VBZ 10_1101-2021_01_08_425864 16 54 impaired impair VBN 10_1101-2021_01_08_425864 16 55 . . . 10_1101-2021_01_08_425864 17 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 17 2 findings finding NNS 10_1101-2021_01_08_425864 17 3 place place VBP 10_1101-2021_01_08_425864 17 4 fatty fatty NN 10_1101-2021_01_08_425864 17 5 acids acid NNS 10_1101-2021_01_08_425864 17 6 in in IN 10_1101-2021_01_08_425864 17 7 the the DT 10_1101-2021_01_08_425864 17 8 centre centre NN 10_1101-2021_01_08_425864 17 9 of of IN 10_1101-2021_01_08_425864 17 10 62 62 CD 10_1101-2021_01_08_425864 17 11 the the DT 10_1101-2021_01_08_425864 17 12 scene scene NN 10_1101-2021_01_08_425864 17 13 regarding regard VBG 10_1101-2021_01_08_425864 17 14 their -PRON- PRP$ 10_1101-2021_01_08_425864 17 15 extraordinary extraordinary JJ 10_1101-2021_01_08_425864 17 16 resistance resistance NN 10_1101-2021_01_08_425864 17 17 to to IN 10_1101-2021_01_08_425864 17 18 nutrient nutrient NN 10_1101-2021_01_08_425864 17 19 - - HYPH 10_1101-2021_01_08_425864 17 20 depleted deplete VBN 10_1101-2021_01_08_425864 17 21 environments environment NNS 10_1101-2021_01_08_425864 17 22 . . . 10_1101-2021_01_08_425864 18 1 63 63 CD 10_1101-2021_01_08_425864 18 2 64 64 CD 10_1101-2021_01_08_425864 18 3 65 65 CD 10_1101-2021_01_08_425864 18 4 .CC .cc CD 10_1101-2021_01_08_425864 18 5 - - : 10_1101-2021_01_08_425864 18 6 BY by IN 10_1101-2021_01_08_425864 18 7 4.0 4.0 CD 10_1101-2021_01_08_425864 18 8 International international JJ 10_1101-2021_01_08_425864 18 9 licensemade licensemade NN 10_1101-2021_01_08_425864 18 10 available available JJ 10_1101-2021_01_08_425864 18 11 under under IN 10_1101-2021_01_08_425864 18 12 a a DT 10_1101-2021_01_08_425864 18 13 ( ( -LRB- 10_1101-2021_01_08_425864 18 14 which which WDT 10_1101-2021_01_08_425864 18 15 was be VBD 10_1101-2021_01_08_425864 18 16 not not RB 10_1101-2021_01_08_425864 18 17 certified certify VBN 10_1101-2021_01_08_425864 18 18 by by IN 10_1101-2021_01_08_425864 18 19 peer peer NN 10_1101-2021_01_08_425864 18 20 review review NN 10_1101-2021_01_08_425864 18 21 ) ) -RRB- 10_1101-2021_01_08_425864 18 22 is be VBZ 10_1101-2021_01_08_425864 18 23 the the DT 10_1101-2021_01_08_425864 18 24 author author NN 10_1101-2021_01_08_425864 18 25 / / SYM 10_1101-2021_01_08_425864 18 26 funder funder NN 10_1101-2021_01_08_425864 18 27 , , , 10_1101-2021_01_08_425864 18 28 who who WP 10_1101-2021_01_08_425864 18 29 has have VBZ 10_1101-2021_01_08_425864 18 30 granted grant VBN 10_1101-2021_01_08_425864 18 31 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 18 32 a a DT 10_1101-2021_01_08_425864 18 33 license license NN 10_1101-2021_01_08_425864 18 34 to to TO 10_1101-2021_01_08_425864 18 35 display display VB 10_1101-2021_01_08_425864 18 36 the the DT 10_1101-2021_01_08_425864 18 37 preprint preprint NN 10_1101-2021_01_08_425864 18 38 in in IN 10_1101-2021_01_08_425864 18 39 perpetuity perpetuity NN 10_1101-2021_01_08_425864 18 40 . . . 10_1101-2021_01_08_425864 19 1 It -PRON- PRP 10_1101-2021_01_08_425864 19 2 is be VBZ 10_1101-2021_01_08_425864 19 3 The the DT 10_1101-2021_01_08_425864 19 4 copyright copyright NN 10_1101-2021_01_08_425864 19 5 holder holder NN 10_1101-2021_01_08_425864 19 6 for for IN 10_1101-2021_01_08_425864 19 7 this this DT 10_1101-2021_01_08_425864 19 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 19 9 version version NN 10_1101-2021_01_08_425864 19 10 posted post VBD 10_1101-2021_01_08_425864 19 11 January January NNP 10_1101-2021_01_08_425864 19 12 8 8 CD 10_1101-2021_01_08_425864 19 13 , , , 10_1101-2021_01_08_425864 19 14 2021 2021 CD 10_1101-2021_01_08_425864 19 15 . . . 10_1101-2021_01_08_425864 19 16 ; ; : 10_1101-2021_01_08_425864 19 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 19 18 : : : 10_1101-2021_01_08_425864 19 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 19 20 preprint preprint NN 10_1101-2021_01_08_425864 19 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 19 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 19 23 66 66 CD 10_1101-2021_01_08_425864 19 24 Introduction introduction NN 10_1101-2021_01_08_425864 19 25 67 67 CD 10_1101-2021_01_08_425864 19 26 T. t. NN 10_1101-2021_01_08_425864 19 27 cruzi cruzi NNS 10_1101-2021_01_08_425864 19 28 , , , 10_1101-2021_01_08_425864 19 29 a a DT 10_1101-2021_01_08_425864 19 30 flagellated flagellated JJ 10_1101-2021_01_08_425864 19 31 parasite parasite NN 10_1101-2021_01_08_425864 19 32 , , , 10_1101-2021_01_08_425864 19 33 is be VBZ 10_1101-2021_01_08_425864 19 34 the the DT 10_1101-2021_01_08_425864 19 35 causative causative JJ 10_1101-2021_01_08_425864 19 36 agent agent NN 10_1101-2021_01_08_425864 19 37 of of IN 10_1101-2021_01_08_425864 19 38 Chagas Chagas NNP 10_1101-2021_01_08_425864 19 39 disease disease NN 10_1101-2021_01_08_425864 19 40 , , , 10_1101-2021_01_08_425864 19 41 a a DT 10_1101-2021_01_08_425864 19 42 neglected neglect VBN 10_1101-2021_01_08_425864 19 43 health health NN 10_1101-2021_01_08_425864 19 44 68 68 CD 10_1101-2021_01_08_425864 19 45 problem problem NN 10_1101-2021_01_08_425864 19 46 endemic endemic RB 10_1101-2021_01_08_425864 19 47 to to IN 10_1101-2021_01_08_425864 19 48 the the DT 10_1101-2021_01_08_425864 19 49 Americas Americas NNPS 10_1101-2021_01_08_425864 19 50 [ [ -LRB- 10_1101-2021_01_08_425864 19 51 1 1 CD 10_1101-2021_01_08_425864 19 52 ] ] -RRB- 10_1101-2021_01_08_425864 19 53 . . . 10_1101-2021_01_08_425864 20 1 The the DT 10_1101-2021_01_08_425864 20 2 parasite parasite NN 10_1101-2021_01_08_425864 20 3 life life NN 10_1101-2021_01_08_425864 20 4 cycle cycle NN 10_1101-2021_01_08_425864 20 5 is be VBZ 10_1101-2021_01_08_425864 20 6 complex complex JJ 10_1101-2021_01_08_425864 20 7 , , , 10_1101-2021_01_08_425864 20 8 alternating alternate VBG 10_1101-2021_01_08_425864 20 9 between between IN 10_1101-2021_01_08_425864 20 10 69 69 CD 10_1101-2021_01_08_425864 20 11 replicative replicative JJ 10_1101-2021_01_08_425864 20 12 and and CC 10_1101-2021_01_08_425864 20 13 non non JJ 10_1101-2021_01_08_425864 20 14 - - JJ 10_1101-2021_01_08_425864 20 15 replicative replicative JJ 10_1101-2021_01_08_425864 20 16 forms form NNS 10_1101-2021_01_08_425864 20 17 in in IN 10_1101-2021_01_08_425864 20 18 two two CD 10_1101-2021_01_08_425864 20 19 types type NNS 10_1101-2021_01_08_425864 20 20 of of IN 10_1101-2021_01_08_425864 20 21 hosts host NNS 10_1101-2021_01_08_425864 20 22 , , , 10_1101-2021_01_08_425864 20 23 mammalians mammalian NNS 10_1101-2021_01_08_425864 20 24 and and CC 10_1101-2021_01_08_425864 20 25 triatomine triatomine JJ 10_1101-2021_01_08_425864 20 26 insects insect NNS 10_1101-2021_01_08_425864 20 27 [ [ -LRB- 10_1101-2021_01_08_425864 20 28 2 2 CD 10_1101-2021_01_08_425864 20 29 ] ] -RRB- 10_1101-2021_01_08_425864 20 30 . . . 10_1101-2021_01_08_425864 21 1 70 70 CD 10_1101-2021_01_08_425864 21 2 In in IN 10_1101-2021_01_08_425864 21 3 mammalian mammalian JJ 10_1101-2021_01_08_425864 21 4 hosts host NNS 10_1101-2021_01_08_425864 21 5 , , , 10_1101-2021_01_08_425864 21 6 two two CD 10_1101-2021_01_08_425864 21 7 primary primary JJ 10_1101-2021_01_08_425864 21 8 forms form NNS 10_1101-2021_01_08_425864 21 9 are be VBP 10_1101-2021_01_08_425864 21 10 recognized recognize VBN 10_1101-2021_01_08_425864 21 11 : : : 10_1101-2021_01_08_425864 21 12 replicative replicative JJ 10_1101-2021_01_08_425864 21 13 intracellular intracellular JJ 10_1101-2021_01_08_425864 21 14 amastigotes amastigote NNS 10_1101-2021_01_08_425864 21 15 and and CC 10_1101-2021_01_08_425864 21 16 71 71 CD 10_1101-2021_01_08_425864 21 17 nondividing nondividing JJ 10_1101-2021_01_08_425864 21 18 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 21 19 , , , 10_1101-2021_01_08_425864 21 20 which which WDT 10_1101-2021_01_08_425864 21 21 are be VBP 10_1101-2021_01_08_425864 21 22 released release VBN 10_1101-2021_01_08_425864 21 23 from from IN 10_1101-2021_01_08_425864 21 24 infected infected JJ 10_1101-2021_01_08_425864 21 25 host host NN 10_1101-2021_01_08_425864 21 26 cells cell NNS 10_1101-2021_01_08_425864 21 27 into into IN 10_1101-2021_01_08_425864 21 28 the the DT 10_1101-2021_01_08_425864 21 29 extracellular extracellular NNP 10_1101-2021_01_08_425864 21 30 72 72 CD 10_1101-2021_01_08_425864 21 31 medium medium NN 10_1101-2021_01_08_425864 21 32 . . . 10_1101-2021_01_08_425864 22 1 After after IN 10_1101-2021_01_08_425864 22 2 being be VBG 10_1101-2021_01_08_425864 22 3 released release VBN 10_1101-2021_01_08_425864 22 4 from from IN 10_1101-2021_01_08_425864 22 5 infected infected JJ 10_1101-2021_01_08_425864 22 6 cells cell NNS 10_1101-2021_01_08_425864 22 7 , , , 10_1101-2021_01_08_425864 22 8 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 22 9 can can MD 10_1101-2021_01_08_425864 22 10 spread spread VB 10_1101-2021_01_08_425864 22 11 the the DT 10_1101-2021_01_08_425864 22 12 infection infection NN 10_1101-2021_01_08_425864 22 13 by by IN 10_1101-2021_01_08_425864 22 14 73 73 CD 10_1101-2021_01_08_425864 22 15 infecting infect VBG 10_1101-2021_01_08_425864 22 16 new new JJ 10_1101-2021_01_08_425864 22 17 cells cell NNS 10_1101-2021_01_08_425864 22 18 , , , 10_1101-2021_01_08_425864 22 19 or or CC 10_1101-2021_01_08_425864 22 20 they -PRON- PRP 10_1101-2021_01_08_425864 22 21 can can MD 10_1101-2021_01_08_425864 22 22 be be VB 10_1101-2021_01_08_425864 22 23 ingested ingest VBN 10_1101-2021_01_08_425864 22 24 by by IN 10_1101-2021_01_08_425864 22 25 a a DT 10_1101-2021_01_08_425864 22 26 triatomine triatomine JJ 10_1101-2021_01_08_425864 22 27 bug bug NN 10_1101-2021_01_08_425864 22 28 during during IN 10_1101-2021_01_08_425864 22 29 its -PRON- PRP$ 10_1101-2021_01_08_425864 22 30 blood blood NN 10_1101-2021_01_08_425864 22 31 meal meal NN 10_1101-2021_01_08_425864 22 32 . . . 10_1101-2021_01_08_425864 23 1 Once once RB 10_1101-2021_01_08_425864 23 2 inside inside IN 10_1101-2021_01_08_425864 23 3 74 74 CD 10_1101-2021_01_08_425864 23 4 the the DT 10_1101-2021_01_08_425864 23 5 invertebrate invertebrate JJ 10_1101-2021_01_08_425864 23 6 host host NN 10_1101-2021_01_08_425864 23 7 , , , 10_1101-2021_01_08_425864 23 8 the the DT 10_1101-2021_01_08_425864 23 9 ingested ingested JJ 10_1101-2021_01_08_425864 23 10 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 23 11 differentiate differentiate JJ 10_1101-2021_01_08_425864 23 12 into into IN 10_1101-2021_01_08_425864 23 13 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 23 14 , , , 10_1101-2021_01_08_425864 23 15 which which WDT 10_1101-2021_01_08_425864 23 16 initiate initiate VBP 10_1101-2021_01_08_425864 23 17 75 75 CD 10_1101-2021_01_08_425864 23 18 their -PRON- PRP$ 10_1101-2021_01_08_425864 23 19 proliferation proliferation NN 10_1101-2021_01_08_425864 23 20 and and CC 10_1101-2021_01_08_425864 23 21 colonization colonization NN 10_1101-2021_01_08_425864 23 22 of of IN 10_1101-2021_01_08_425864 23 23 the the DT 10_1101-2021_01_08_425864 23 24 insect insect JJ 10_1101-2021_01_08_425864 23 25 digestive digestive JJ 10_1101-2021_01_08_425864 23 26 tract tract NN 10_1101-2021_01_08_425864 23 27 [ [ -LRB- 10_1101-2021_01_08_425864 23 28 3 3 CD 10_1101-2021_01_08_425864 23 29 ] ] -RRB- 10_1101-2021_01_08_425864 23 30 . . . 10_1101-2021_01_08_425864 24 1 Once once IN 10_1101-2021_01_08_425864 24 2 the the DT 10_1101-2021_01_08_425864 24 3 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 24 4 reach reach VBP 10_1101-2021_01_08_425864 24 5 the the DT 10_1101-2021_01_08_425864 24 6 76 76 CD 10_1101-2021_01_08_425864 24 7 final final JJ 10_1101-2021_01_08_425864 24 8 portion portion NN 10_1101-2021_01_08_425864 24 9 of of IN 10_1101-2021_01_08_425864 24 10 the the DT 10_1101-2021_01_08_425864 24 11 digestive digestive JJ 10_1101-2021_01_08_425864 24 12 tube tube NN 10_1101-2021_01_08_425864 24 13 , , , 10_1101-2021_01_08_425864 24 14 they -PRON- PRP 10_1101-2021_01_08_425864 24 15 initiate initiate VBP 10_1101-2021_01_08_425864 24 16 differentiation differentiation NN 10_1101-2021_01_08_425864 24 17 into into IN 10_1101-2021_01_08_425864 24 18 non non JJ 10_1101-2021_01_08_425864 24 19 - - JJ 10_1101-2021_01_08_425864 24 20 proliferative proliferative JJ 10_1101-2021_01_08_425864 24 21 , , , 10_1101-2021_01_08_425864 24 22 infective infective JJ 10_1101-2021_01_08_425864 24 23 77 77 CD 10_1101-2021_01_08_425864 24 24 metacyclic metacyclic JJ 10_1101-2021_01_08_425864 24 25 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 24 26 . . . 10_1101-2021_01_08_425864 25 1 These these DT 10_1101-2021_01_08_425864 25 2 forms form NNS 10_1101-2021_01_08_425864 25 3 will will MD 10_1101-2021_01_08_425864 25 4 be be VB 10_1101-2021_01_08_425864 25 5 expelled expel VBN 10_1101-2021_01_08_425864 25 6 during during IN 10_1101-2021_01_08_425864 25 7 a a DT 10_1101-2021_01_08_425864 25 8 new new JJ 10_1101-2021_01_08_425864 25 9 blood blood NN 10_1101-2021_01_08_425864 25 10 meal meal NN 10_1101-2021_01_08_425864 25 11 and and CC 10_1101-2021_01_08_425864 25 12 will will MD 10_1101-2021_01_08_425864 25 13 be be VB 10_1101-2021_01_08_425864 25 14 able able JJ 10_1101-2021_01_08_425864 25 15 78 78 CD 10_1101-2021_01_08_425864 25 16 to to TO 10_1101-2021_01_08_425864 25 17 infect infect VB 10_1101-2021_01_08_425864 25 18 a a DT 10_1101-2021_01_08_425864 25 19 new new JJ 10_1101-2021_01_08_425864 25 20 vertebrate vertebrate NN 10_1101-2021_01_08_425864 25 21 host host NN 10_1101-2021_01_08_425864 25 22 [ [ -LRB- 10_1101-2021_01_08_425864 25 23 2,4–6 2,4–6 CD 10_1101-2021_01_08_425864 25 24 ] ] -RRB- 10_1101-2021_01_08_425864 25 25 . . . 10_1101-2021_01_08_425864 26 1 79 79 CD 10_1101-2021_01_08_425864 26 2 The the DT 10_1101-2021_01_08_425864 26 3 diversity diversity NN 10_1101-2021_01_08_425864 26 4 of of IN 10_1101-2021_01_08_425864 26 5 environments environment NNS 10_1101-2021_01_08_425864 26 6 through through IN 10_1101-2021_01_08_425864 26 7 which which WDT 10_1101-2021_01_08_425864 26 8 T. t. NN 10_1101-2021_01_08_425864 26 9 cruzi cruzi NNS 10_1101-2021_01_08_425864 26 10 passes pass VBZ 10_1101-2021_01_08_425864 26 11 during during IN 10_1101-2021_01_08_425864 26 12 its -PRON- PRP$ 10_1101-2021_01_08_425864 26 13 life life NN 10_1101-2021_01_08_425864 26 14 cycle cycle NN 10_1101-2021_01_08_425864 26 15 ( ( -LRB- 10_1101-2021_01_08_425864 26 16 i.e. i.e. FW 10_1101-2021_01_08_425864 26 17 , , , 10_1101-2021_01_08_425864 26 18 the the DT 10_1101-2021_01_08_425864 26 19 80 80 CD 10_1101-2021_01_08_425864 26 20 digestive digestive JJ 10_1101-2021_01_08_425864 26 21 tube tube NN 10_1101-2021_01_08_425864 26 22 of of IN 10_1101-2021_01_08_425864 26 23 the the DT 10_1101-2021_01_08_425864 26 24 insect insect JJ 10_1101-2021_01_08_425864 26 25 vector vector NN 10_1101-2021_01_08_425864 26 26 , , , 10_1101-2021_01_08_425864 26 27 the the DT 10_1101-2021_01_08_425864 26 28 bloodstream bloodstream NN 10_1101-2021_01_08_425864 26 29 and and CC 10_1101-2021_01_08_425864 26 30 the the DT 10_1101-2021_01_08_425864 26 31 mammalian mammalian NNP 10_1101-2021_01_08_425864 26 32 cells cell NNS 10_1101-2021_01_08_425864 26 33 cytoplasm cytoplasm NNP 10_1101-2021_01_08_425864 26 34 ) ) -RRB- 10_1101-2021_01_08_425864 26 35 subjects subject VBZ 10_1101-2021_01_08_425864 26 36 it -PRON- PRP 10_1101-2021_01_08_425864 26 37 81 81 CD 10_1101-2021_01_08_425864 26 38 to to IN 10_1101-2021_01_08_425864 26 39 different different JJ 10_1101-2021_01_08_425864 26 40 levels level NNS 10_1101-2021_01_08_425864 26 41 of of IN 10_1101-2021_01_08_425864 26 42 nutrient nutrient NN 10_1101-2021_01_08_425864 26 43 availability availability NN 10_1101-2021_01_08_425864 26 44 [ [ -LRB- 10_1101-2021_01_08_425864 26 45 3,7 3,7 CD 10_1101-2021_01_08_425864 26 46 ] ] -RRB- 10_1101-2021_01_08_425864 26 47 . . . 10_1101-2021_01_08_425864 27 1 Therefore therefore RB 10_1101-2021_01_08_425864 27 2 , , , 10_1101-2021_01_08_425864 27 3 this this DT 10_1101-2021_01_08_425864 27 4 organism organism NN 10_1101-2021_01_08_425864 27 5 evolved evolve VBD 10_1101-2021_01_08_425864 27 6 a a DT 10_1101-2021_01_08_425864 27 7 robust robust JJ 10_1101-2021_01_08_425864 27 8 , , , 10_1101-2021_01_08_425864 27 9 flexible flexible JJ 10_1101-2021_01_08_425864 27 10 82 82 CD 10_1101-2021_01_08_425864 27 11 and and CC 10_1101-2021_01_08_425864 27 12 efficient efficient JJ 10_1101-2021_01_08_425864 27 13 metabolism metabolism NN 10_1101-2021_01_08_425864 27 14 [ [ -LRB- 10_1101-2021_01_08_425864 27 15 5,8 5,8 CD 10_1101-2021_01_08_425864 27 16 ] ] -RRB- 10_1101-2021_01_08_425864 27 17 . . . 10_1101-2021_01_08_425864 28 1 As as IN 10_1101-2021_01_08_425864 28 2 an an DT 10_1101-2021_01_08_425864 28 3 example example NN 10_1101-2021_01_08_425864 28 4 , , , 10_1101-2021_01_08_425864 28 5 it -PRON- PRP 10_1101-2021_01_08_425864 28 6 was be VBD 10_1101-2021_01_08_425864 28 7 recognized recognize VBN 10_1101-2021_01_08_425864 28 8 early early RB 10_1101-2021_01_08_425864 28 9 on on IN 10_1101-2021_01_08_425864 28 10 that that DT 10_1101-2021_01_08_425864 28 11 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 28 12 are be VBP 10_1101-2021_01_08_425864 28 13 able able JJ 10_1101-2021_01_08_425864 28 14 83 83 CD 10_1101-2021_01_08_425864 28 15 to to TO 10_1101-2021_01_08_425864 28 16 rapidly rapidly RB 10_1101-2021_01_08_425864 28 17 switch switch VB 10_1101-2021_01_08_425864 28 18 their -PRON- PRP$ 10_1101-2021_01_08_425864 28 19 metabolism metabolism NN 10_1101-2021_01_08_425864 28 20 , , , 10_1101-2021_01_08_425864 28 21 allowing allow VBG 10_1101-2021_01_08_425864 28 22 the the DT 10_1101-2021_01_08_425864 28 23 consumption consumption NN 10_1101-2021_01_08_425864 28 24 of of IN 10_1101-2021_01_08_425864 28 25 carbohydrates carbohydrate NNS 10_1101-2021_01_08_425864 28 26 and and CC 10_1101-2021_01_08_425864 28 27 different different JJ 10_1101-2021_01_08_425864 28 28 amino amino NN 10_1101-2021_01_08_425864 28 29 84 84 CD 10_1101-2021_01_08_425864 28 30 acids acid NNS 10_1101-2021_01_08_425864 28 31 [ [ -LRB- 10_1101-2021_01_08_425864 28 32 9,10 9,10 NN 10_1101-2021_01_08_425864 28 33 ] ] -RRB- 10_1101-2021_01_08_425864 28 34 . . . 10_1101-2021_01_08_425864 29 1 Several several JJ 10_1101-2021_01_08_425864 29 2 studies study NNS 10_1101-2021_01_08_425864 29 3 identified identify VBN 10_1101-2021_01_08_425864 29 4 aspartate aspartate NN 10_1101-2021_01_08_425864 29 5 , , , 10_1101-2021_01_08_425864 29 6 asparagine asparagine JJ 10_1101-2021_01_08_425864 29 7 , , , 10_1101-2021_01_08_425864 29 8 glutamate glutamate NNP 10_1101-2021_01_08_425864 29 9 [ [ -LRB- 10_1101-2021_01_08_425864 29 10 11 11 CD 10_1101-2021_01_08_425864 29 11 ] ] -RRB- 10_1101-2021_01_08_425864 29 12 , , , 10_1101-2021_01_08_425864 29 13 proline proline NNP 10_1101-2021_01_08_425864 29 14 [ [ -LRB- 10_1101-2021_01_08_425864 29 15 12–14 12–14 CD 10_1101-2021_01_08_425864 29 16 ] ] -RRB- 10_1101-2021_01_08_425864 29 17 , , , 10_1101-2021_01_08_425864 29 18 85 85 CD 10_1101-2021_01_08_425864 29 19 histidine histidine NN 10_1101-2021_01_08_425864 29 20 [ [ -LRB- 10_1101-2021_01_08_425864 29 21 15 15 CD 10_1101-2021_01_08_425864 29 22 ] ] -RRB- 10_1101-2021_01_08_425864 29 23 , , , 10_1101-2021_01_08_425864 29 24 alanine alanine NN 10_1101-2021_01_08_425864 29 25 [ [ -LRB- 10_1101-2021_01_08_425864 29 26 11,16 11,16 CD 10_1101-2021_01_08_425864 29 27 ] ] -RRB- 10_1101-2021_01_08_425864 29 28 and and CC 10_1101-2021_01_08_425864 29 29 glutamine glutamine NN 10_1101-2021_01_08_425864 29 30 [ [ -LRB- 10_1101-2021_01_08_425864 29 31 11,17 11,17 CD 10_1101-2021_01_08_425864 29 32 ] ] -RRB- 10_1101-2021_01_08_425864 29 33 as as IN 10_1101-2021_01_08_425864 29 34 oxidisable oxidisable JJ 10_1101-2021_01_08_425864 29 35 energy energy NN 10_1101-2021_01_08_425864 29 36 sources source NNS 10_1101-2021_01_08_425864 29 37 . . . 10_1101-2021_01_08_425864 30 1 Despite despite IN 10_1101-2021_01_08_425864 30 2 the the DT 10_1101-2021_01_08_425864 30 3 86 86 CD 10_1101-2021_01_08_425864 30 4 quantity quantity NN 10_1101-2021_01_08_425864 30 5 of of IN 10_1101-2021_01_08_425864 30 6 accumulated accumulate VBN 10_1101-2021_01_08_425864 30 7 information information NN 10_1101-2021_01_08_425864 30 8 on on IN 10_1101-2021_01_08_425864 30 9 amino amino JJ 10_1101-2021_01_08_425864 30 10 acid acid NN 10_1101-2021_01_08_425864 30 11 and and CC 10_1101-2021_01_08_425864 30 12 carbohydrate carbohydrate NN 10_1101-2021_01_08_425864 30 13 consumption consumption NN 10_1101-2021_01_08_425864 30 14 , , , 10_1101-2021_01_08_425864 30 15 little little JJ 10_1101-2021_01_08_425864 30 16 is be VBZ 10_1101-2021_01_08_425864 30 17 known know VBN 10_1101-2021_01_08_425864 30 18 87 87 CD 10_1101-2021_01_08_425864 30 19 about about IN 10_1101-2021_01_08_425864 30 20 how how WRB 10_1101-2021_01_08_425864 30 21 T. t. NN 10_1101-2021_01_08_425864 30 22 cruzi cruzi NNS 10_1101-2021_01_08_425864 30 23 uses use VBZ 10_1101-2021_01_08_425864 30 24 fatty fatty NN 10_1101-2021_01_08_425864 30 25 acids acid NNS 10_1101-2021_01_08_425864 30 26 and and CC 10_1101-2021_01_08_425864 30 27 how how WRB 10_1101-2021_01_08_425864 30 28 these these DT 10_1101-2021_01_08_425864 30 29 compounds compound NNS 10_1101-2021_01_08_425864 30 30 contribute contribute VBP 10_1101-2021_01_08_425864 30 31 to to IN 10_1101-2021_01_08_425864 30 32 the the DT 10_1101-2021_01_08_425864 30 33 parasite´s parasite´s NNP 10_1101-2021_01_08_425864 30 34 metabolism metabolism NN 10_1101-2021_01_08_425864 30 35 88 88 CD 10_1101-2021_01_08_425864 30 36 and and CC 10_1101-2021_01_08_425864 30 37 survival survival NN 10_1101-2021_01_08_425864 30 38 . . . 10_1101-2021_01_08_425864 31 1 In in IN 10_1101-2021_01_08_425864 31 2 this this DT 10_1101-2021_01_08_425864 31 3 study study NN 10_1101-2021_01_08_425864 31 4 , , , 10_1101-2021_01_08_425864 31 5 we -PRON- PRP 10_1101-2021_01_08_425864 31 6 explore explore VBP 10_1101-2021_01_08_425864 31 7 fatty fatty NN 10_1101-2021_01_08_425864 31 8 acid acid NN 10_1101-2021_01_08_425864 31 9 metabolism metabolism NN 10_1101-2021_01_08_425864 31 10 in in IN 10_1101-2021_01_08_425864 31 11 T. T. NNP 10_1101-2021_01_08_425864 31 12 cruzi cruzi NNS 10_1101-2021_01_08_425864 31 13 . . . 10_1101-2021_01_08_425864 32 1 We -PRON- PRP 10_1101-2021_01_08_425864 32 2 also also RB 10_1101-2021_01_08_425864 32 3 address address VBP 10_1101-2021_01_08_425864 32 4 fatty fatty NN 10_1101-2021_01_08_425864 32 5 acid acid NN 10_1101-2021_01_08_425864 32 6 89 89 CD 10_1101-2021_01_08_425864 32 7 regulation regulation NN 10_1101-2021_01_08_425864 32 8 by by IN 10_1101-2021_01_08_425864 32 9 external external JJ 10_1101-2021_01_08_425864 32 10 glucose glucose NN 10_1101-2021_01_08_425864 32 11 levels level NNS 10_1101-2021_01_08_425864 32 12 and and CC 10_1101-2021_01_08_425864 32 13 the the DT 10_1101-2021_01_08_425864 32 14 involvement involvement NN 10_1101-2021_01_08_425864 32 15 of of IN 10_1101-2021_01_08_425864 32 16 their -PRON- PRP$ 10_1101-2021_01_08_425864 32 17 oxidation oxidation NN 10_1101-2021_01_08_425864 32 18 in in IN 10_1101-2021_01_08_425864 32 19 the the DT 10_1101-2021_01_08_425864 32 20 replication replication NN 10_1101-2021_01_08_425864 32 21 and and CC 10_1101-2021_01_08_425864 32 22 90 90 CD 10_1101-2021_01_08_425864 32 23 differentiation differentiation NN 10_1101-2021_01_08_425864 32 24 of of IN 10_1101-2021_01_08_425864 32 25 T. t. NN 10_1101-2021_01_08_425864 32 26 cruzi cruzi NNS 10_1101-2021_01_08_425864 32 27 insect insect NN 10_1101-2021_01_08_425864 32 28 stages stage NNS 10_1101-2021_01_08_425864 32 29 . . . 10_1101-2021_01_08_425864 33 1 91 91 CD 10_1101-2021_01_08_425864 33 2 92 92 CD 10_1101-2021_01_08_425864 33 3 Methods method NNS 10_1101-2021_01_08_425864 33 4 93 93 CD 10_1101-2021_01_08_425864 33 5 Parasites parasite NNS 10_1101-2021_01_08_425864 33 6 94 94 CD 10_1101-2021_01_08_425864 33 7 Epimastigotes Epimastigotes NNPS 10_1101-2021_01_08_425864 33 8 of of IN 10_1101-2021_01_08_425864 33 9 T. t. NN 10_1101-2021_01_08_425864 33 10 cruzi cruzi NNS 10_1101-2021_01_08_425864 33 11 strain strain NN 10_1101-2021_01_08_425864 33 12 CL CL NNP 10_1101-2021_01_08_425864 33 13 clone clone NN 10_1101-2021_01_08_425864 33 14 14 14 CD 10_1101-2021_01_08_425864 33 15 were be VBD 10_1101-2021_01_08_425864 33 16 maintained maintain VBN 10_1101-2021_01_08_425864 33 17 in in IN 10_1101-2021_01_08_425864 33 18 the the DT 10_1101-2021_01_08_425864 33 19 exponential exponential JJ 10_1101-2021_01_08_425864 33 20 growth growth NN 10_1101-2021_01_08_425864 33 21 phase phase NN 10_1101-2021_01_08_425864 33 22 95 95 CD 10_1101-2021_01_08_425864 33 23 by by IN 10_1101-2021_01_08_425864 33 24 sub sub JJ 10_1101-2021_01_08_425864 33 25 - - JJ 10_1101-2021_01_08_425864 33 26 culturing culture VBG 10_1101-2021_01_08_425864 33 27 them -PRON- PRP 10_1101-2021_01_08_425864 33 28 for for IN 10_1101-2021_01_08_425864 33 29 48 48 CD 10_1101-2021_01_08_425864 33 30 h h NN 10_1101-2021_01_08_425864 33 31 in in IN 10_1101-2021_01_08_425864 33 32 Liver Liver NNP 10_1101-2021_01_08_425864 33 33 Infusion Infusion NNP 10_1101-2021_01_08_425864 33 34 Tryptose Tryptose NNP 10_1101-2021_01_08_425864 33 35 ( ( -LRB- 10_1101-2021_01_08_425864 33 36 LIT LIT NNP 10_1101-2021_01_08_425864 33 37 ) ) -RRB- 10_1101-2021_01_08_425864 33 38 medium medium NN 10_1101-2021_01_08_425864 33 39 at at IN 10_1101-2021_01_08_425864 33 40 28 28 CD 10_1101-2021_01_08_425864 33 41 ° ° , 10_1101-2021_01_08_425864 33 42 C C NNP 10_1101-2021_01_08_425864 33 43 [ [ -LRB- 10_1101-2021_01_08_425864 33 44 18 18 CD 10_1101-2021_01_08_425864 33 45 ] ] -RRB- 10_1101-2021_01_08_425864 33 46 . . . 10_1101-2021_01_08_425864 34 1 Metacyclic metacyclic JJ 10_1101-2021_01_08_425864 34 2 96 96 CD 10_1101-2021_01_08_425864 34 3 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 34 4 were be VBD 10_1101-2021_01_08_425864 34 5 obtained obtain VBN 10_1101-2021_01_08_425864 34 6 through through IN 10_1101-2021_01_08_425864 34 7 the the DT 10_1101-2021_01_08_425864 34 8 differentiation differentiation NN 10_1101-2021_01_08_425864 34 9 of of IN 10_1101-2021_01_08_425864 34 10 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 34 11 at at IN 10_1101-2021_01_08_425864 34 12 the the DT 10_1101-2021_01_08_425864 34 13 stationary stationary JJ 10_1101-2021_01_08_425864 34 14 growth growth NN 10_1101-2021_01_08_425864 34 15 97 97 CD 10_1101-2021_01_08_425864 34 16 phase phase NN 10_1101-2021_01_08_425864 34 17 in in IN 10_1101-2021_01_08_425864 34 18 TAU-3AAG TAU-3AAG NNP 10_1101-2021_01_08_425864 34 19 ( ( -LRB- 10_1101-2021_01_08_425864 34 20 Triatomine Triatomine NNP 10_1101-2021_01_08_425864 34 21 Artificial Artificial NNP 10_1101-2021_01_08_425864 34 22 Urine Urine NNP 10_1101-2021_01_08_425864 34 23 supplemented supplement VBN 10_1101-2021_01_08_425864 34 24 with with IN 10_1101-2021_01_08_425864 34 25 10 10 CD 10_1101-2021_01_08_425864 34 26 mM mm NN 10_1101-2021_01_08_425864 34 27 proline proline NN 10_1101-2021_01_08_425864 34 28 , , , 10_1101-2021_01_08_425864 34 29 50 50 CD 10_1101-2021_01_08_425864 34 30 mM mM NNP 10_1101-2021_01_08_425864 34 31 98 98 CD 10_1101-2021_01_08_425864 34 32 glutamate glutamate NN 10_1101-2021_01_08_425864 34 33 , , , 10_1101-2021_01_08_425864 34 34 2 2 CD 10_1101-2021_01_08_425864 34 35 mM mM NNP 10_1101-2021_01_08_425864 34 36 aspartate aspartate NN 10_1101-2021_01_08_425864 34 37 and and CC 10_1101-2021_01_08_425864 34 38 10 10 CD 10_1101-2021_01_08_425864 34 39 mM mm NN 10_1101-2021_01_08_425864 34 40 glucose glucose NN 10_1101-2021_01_08_425864 34 41 ) ) -RRB- 10_1101-2021_01_08_425864 34 42 as as IN 10_1101-2021_01_08_425864 34 43 previously previously RB 10_1101-2021_01_08_425864 34 44 reported report VBN 10_1101-2021_01_08_425864 34 45 [ [ -LRB- 10_1101-2021_01_08_425864 34 46 19 19 CD 10_1101-2021_01_08_425864 34 47 ] ] -RRB- 10_1101-2021_01_08_425864 34 48 . . . 10_1101-2021_01_08_425864 35 1 .CC .CC NFP 10_1101-2021_01_08_425864 35 2 - - : 10_1101-2021_01_08_425864 35 3 BY by IN 10_1101-2021_01_08_425864 35 4 4.0 4.0 CD 10_1101-2021_01_08_425864 35 5 International international JJ 10_1101-2021_01_08_425864 35 6 licensemade licensemade NN 10_1101-2021_01_08_425864 35 7 available available JJ 10_1101-2021_01_08_425864 35 8 under under IN 10_1101-2021_01_08_425864 35 9 a a DT 10_1101-2021_01_08_425864 35 10 ( ( -LRB- 10_1101-2021_01_08_425864 35 11 which which WDT 10_1101-2021_01_08_425864 35 12 was be VBD 10_1101-2021_01_08_425864 35 13 not not RB 10_1101-2021_01_08_425864 35 14 certified certify VBN 10_1101-2021_01_08_425864 35 15 by by IN 10_1101-2021_01_08_425864 35 16 peer peer NN 10_1101-2021_01_08_425864 35 17 review review NN 10_1101-2021_01_08_425864 35 18 ) ) -RRB- 10_1101-2021_01_08_425864 35 19 is be VBZ 10_1101-2021_01_08_425864 35 20 the the DT 10_1101-2021_01_08_425864 35 21 author author NN 10_1101-2021_01_08_425864 35 22 / / SYM 10_1101-2021_01_08_425864 35 23 funder funder NN 10_1101-2021_01_08_425864 35 24 , , , 10_1101-2021_01_08_425864 35 25 who who WP 10_1101-2021_01_08_425864 35 26 has have VBZ 10_1101-2021_01_08_425864 35 27 granted grant VBN 10_1101-2021_01_08_425864 35 28 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 35 29 a a DT 10_1101-2021_01_08_425864 35 30 license license NN 10_1101-2021_01_08_425864 35 31 to to TO 10_1101-2021_01_08_425864 35 32 display display VB 10_1101-2021_01_08_425864 35 33 the the DT 10_1101-2021_01_08_425864 35 34 preprint preprint NN 10_1101-2021_01_08_425864 35 35 in in IN 10_1101-2021_01_08_425864 35 36 perpetuity perpetuity NN 10_1101-2021_01_08_425864 35 37 . . . 10_1101-2021_01_08_425864 36 1 It -PRON- PRP 10_1101-2021_01_08_425864 36 2 is be VBZ 10_1101-2021_01_08_425864 36 3 The the DT 10_1101-2021_01_08_425864 36 4 copyright copyright NN 10_1101-2021_01_08_425864 36 5 holder holder NN 10_1101-2021_01_08_425864 36 6 for for IN 10_1101-2021_01_08_425864 36 7 this this DT 10_1101-2021_01_08_425864 36 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 36 9 version version NN 10_1101-2021_01_08_425864 36 10 posted post VBD 10_1101-2021_01_08_425864 36 11 January January NNP 10_1101-2021_01_08_425864 36 12 8 8 CD 10_1101-2021_01_08_425864 36 13 , , , 10_1101-2021_01_08_425864 36 14 2021 2021 CD 10_1101-2021_01_08_425864 36 15 . . . 10_1101-2021_01_08_425864 36 16 ; ; : 10_1101-2021_01_08_425864 36 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 36 18 : : : 10_1101-2021_01_08_425864 36 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 36 20 preprint preprint NN 10_1101-2021_01_08_425864 36 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 36 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 36 23 99 99 CD 10_1101-2021_01_08_425864 36 24 100 100 CD 10_1101-2021_01_08_425864 36 25 Fatty Fatty NNP 10_1101-2021_01_08_425864 36 26 acid acid JJ 10_1101-2021_01_08_425864 36 27 oxidation oxidation NN 10_1101-2021_01_08_425864 36 28 assays assays RB 10_1101-2021_01_08_425864 36 29 101 101 CD 10_1101-2021_01_08_425864 36 30 Preparation Preparation NNP 10_1101-2021_01_08_425864 36 31 of of IN 10_1101-2021_01_08_425864 36 32 palmitate palmitate NN 10_1101-2021_01_08_425864 36 33 - - HYPH 10_1101-2021_01_08_425864 36 34 BSA BSA NNP 10_1101-2021_01_08_425864 36 35 conjugates conjugate NNS 10_1101-2021_01_08_425864 36 36 . . . 10_1101-2021_01_08_425864 37 1 Sodium sodium NN 10_1101-2021_01_08_425864 37 2 palmitate palmitate NN 10_1101-2021_01_08_425864 37 3 at at IN 10_1101-2021_01_08_425864 37 4 70 70 CD 10_1101-2021_01_08_425864 37 5 mM mM NNP 10_1101-2021_01_08_425864 37 6 was be VBD 10_1101-2021_01_08_425864 37 7 solubilized solubilize VBN 10_1101-2021_01_08_425864 37 8 in in IN 10_1101-2021_01_08_425864 37 9 water water NN 10_1101-2021_01_08_425864 37 10 by by IN 10_1101-2021_01_08_425864 37 11 102 102 CD 10_1101-2021_01_08_425864 37 12 heating heat VBG 10_1101-2021_01_08_425864 37 13 it -PRON- PRP 10_1101-2021_01_08_425864 37 14 up up IN 10_1101-2021_01_08_425864 37 15 to to TO 10_1101-2021_01_08_425864 37 16 70 70 CD 10_1101-2021_01_08_425864 37 17 ° ° , 10_1101-2021_01_08_425864 37 18 C C NNP 10_1101-2021_01_08_425864 37 19 . . . 10_1101-2021_01_08_425864 38 1 BSA bsa DT 10_1101-2021_01_08_425864 38 2 free free JJ 10_1101-2021_01_08_425864 38 3 fatty fatty NN 10_1101-2021_01_08_425864 38 4 acids acid NNS 10_1101-2021_01_08_425864 38 5 ( ( -LRB- 10_1101-2021_01_08_425864 38 6 FFA FFA NNP 10_1101-2021_01_08_425864 38 7 BSA BSA NNP 10_1101-2021_01_08_425864 38 8 ) ) -RRB- 10_1101-2021_01_08_425864 38 9 ( ( -LRB- 10_1101-2021_01_08_425864 38 10 Sigma Sigma NNP 10_1101-2021_01_08_425864 38 11 ® ® NNP 10_1101-2021_01_08_425864 38 12 ) ) -RRB- 10_1101-2021_01_08_425864 38 13 was be VBD 10_1101-2021_01_08_425864 38 14 dissolved dissolve VBN 10_1101-2021_01_08_425864 38 15 in in IN 10_1101-2021_01_08_425864 38 16 PBS PBS NNP 10_1101-2021_01_08_425864 38 17 and and CC 10_1101-2021_01_08_425864 38 18 warmed warm VBD 10_1101-2021_01_08_425864 38 19 103 103 CD 10_1101-2021_01_08_425864 38 20 up up IN 10_1101-2021_01_08_425864 38 21 to to TO 10_1101-2021_01_08_425864 38 22 37 37 CD 10_1101-2021_01_08_425864 38 23 ° ° , 10_1101-2021_01_08_425864 38 24 C C NNP 10_1101-2021_01_08_425864 38 25 with with IN 10_1101-2021_01_08_425864 38 26 continuous continuous JJ 10_1101-2021_01_08_425864 38 27 stirring stirring NN 10_1101-2021_01_08_425864 38 28 . . . 10_1101-2021_01_08_425864 39 1 Solubilized solubilized JJ 10_1101-2021_01_08_425864 39 2 palmitate palmitate NN 10_1101-2021_01_08_425864 39 3 was be VBD 10_1101-2021_01_08_425864 39 4 added add VBN 10_1101-2021_01_08_425864 39 5 to to IN 10_1101-2021_01_08_425864 39 6 BSA BSA NNP 10_1101-2021_01_08_425864 39 7 at at IN 10_1101-2021_01_08_425864 39 8 37 37 CD 10_1101-2021_01_08_425864 39 9 ° ° , 10_1101-2021_01_08_425864 39 10 C C NNP 10_1101-2021_01_08_425864 39 11 with with IN 10_1101-2021_01_08_425864 39 12 104 104 CD 10_1101-2021_01_08_425864 39 13 continuous continuous JJ 10_1101-2021_01_08_425864 39 14 stirring stirring NN 10_1101-2021_01_08_425864 39 15 ( ( -LRB- 10_1101-2021_01_08_425864 39 16 for for IN 10_1101-2021_01_08_425864 39 17 a a DT 10_1101-2021_01_08_425864 39 18 final final JJ 10_1101-2021_01_08_425864 39 19 concentration concentration NN 10_1101-2021_01_08_425864 39 20 of of IN 10_1101-2021_01_08_425864 39 21 5 5 CD 10_1101-2021_01_08_425864 39 22 mM mM NNP 10_1101-2021_01_08_425864 39 23 in in IN 10_1101-2021_01_08_425864 39 24 7 7 CD 10_1101-2021_01_08_425864 39 25 % % NN 10_1101-2021_01_08_425864 39 26 BSA BSA NNP 10_1101-2021_01_08_425864 39 27 ) ) -RRB- 10_1101-2021_01_08_425864 39 28 . . . 10_1101-2021_01_08_425864 40 1 The the DT 10_1101-2021_01_08_425864 40 2 conjugated conjugate VBN 10_1101-2021_01_08_425864 40 3 palmitate palmitate NN 10_1101-2021_01_08_425864 40 4 - - : 10_1101-2021_01_08_425864 40 5 BSA BSA NNP 10_1101-2021_01_08_425864 40 6 105 105 CD 10_1101-2021_01_08_425864 40 7 was be VBD 10_1101-2021_01_08_425864 40 8 aliquoted aliquote VBN 10_1101-2021_01_08_425864 40 9 and and CC 10_1101-2021_01_08_425864 40 10 stored store VBN 10_1101-2021_01_08_425864 40 11 at at IN 10_1101-2021_01_08_425864 40 12 −80 −80 NNP 10_1101-2021_01_08_425864 40 13 ° ° , 10_1101-2021_01_08_425864 40 14 C C NNP 10_1101-2021_01_08_425864 40 15 [ [ -LRB- 10_1101-2021_01_08_425864 40 16 20 20 CD 10_1101-2021_01_08_425864 40 17 ] ] -RRB- 10_1101-2021_01_08_425864 40 18 . . . 10_1101-2021_01_08_425864 41 1 106 106 CD 10_1101-2021_01_08_425864 41 2 107 107 CD 10_1101-2021_01_08_425864 41 3 CO2 CO2 NNP 10_1101-2021_01_08_425864 41 4 production production NN 10_1101-2021_01_08_425864 41 5 from from IN 10_1101-2021_01_08_425864 41 6 oxidisable oxidisable JJ 10_1101-2021_01_08_425864 41 7 carbon carbon NN 10_1101-2021_01_08_425864 41 8 sources source NNS 10_1101-2021_01_08_425864 41 9 . . . 10_1101-2021_01_08_425864 42 1 To to TO 10_1101-2021_01_08_425864 42 2 test test VB 10_1101-2021_01_08_425864 42 3 the the DT 10_1101-2021_01_08_425864 42 4 production production NN 10_1101-2021_01_08_425864 42 5 of of IN 10_1101-2021_01_08_425864 42 6 CO2 CO2 NNP 10_1101-2021_01_08_425864 42 7 from from IN 10_1101-2021_01_08_425864 42 8 palmitate palmitate NN 10_1101-2021_01_08_425864 42 9 , , , 10_1101-2021_01_08_425864 42 10 108 108 CD 10_1101-2021_01_08_425864 42 11 glucose glucose NN 10_1101-2021_01_08_425864 42 12 or or CC 10_1101-2021_01_08_425864 42 13 histidine histidine NN 10_1101-2021_01_08_425864 42 14 , , , 10_1101-2021_01_08_425864 42 15 exponentially exponentially RB 10_1101-2021_01_08_425864 42 16 growing grow VBG 10_1101-2021_01_08_425864 42 17 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 42 18 ( ( -LRB- 10_1101-2021_01_08_425864 42 19 5x107 5x107 CD 10_1101-2021_01_08_425864 42 20 mL-1 mL-1 NNP 10_1101-2021_01_08_425864 42 21 ) ) -RRB- 10_1101-2021_01_08_425864 42 22 were be VBD 10_1101-2021_01_08_425864 42 23 washed wash VBN 10_1101-2021_01_08_425864 42 24 twice twice RB 10_1101-2021_01_08_425864 42 25 in in IN 10_1101-2021_01_08_425864 42 26 PBS PBS NNP 10_1101-2021_01_08_425864 42 27 109 109 CD 10_1101-2021_01_08_425864 42 28 and and CC 10_1101-2021_01_08_425864 42 29 incubated incubate VBD 10_1101-2021_01_08_425864 42 30 for for IN 10_1101-2021_01_08_425864 42 31 different different JJ 10_1101-2021_01_08_425864 42 32 times time NNS 10_1101-2021_01_08_425864 42 33 ( ( -LRB- 10_1101-2021_01_08_425864 42 34 0 0 CD 10_1101-2021_01_08_425864 42 35 , , , 10_1101-2021_01_08_425864 42 36 30 30 CD 10_1101-2021_01_08_425864 42 37 , , , 10_1101-2021_01_08_425864 42 38 60 60 CD 10_1101-2021_01_08_425864 42 39 and and CC 10_1101-2021_01_08_425864 42 40 120 120 CD 10_1101-2021_01_08_425864 42 41 min min NN 10_1101-2021_01_08_425864 42 42 ) ) -RRB- 10_1101-2021_01_08_425864 42 43 in in IN 10_1101-2021_01_08_425864 42 44 the the DT 10_1101-2021_01_08_425864 42 45 presence presence NN 10_1101-2021_01_08_425864 42 46 of of IN 10_1101-2021_01_08_425864 42 47 0.1 0.1 CD 10_1101-2021_01_08_425864 42 48 mM mM NNP 10_1101-2021_01_08_425864 42 49 of of IN 10_1101-2021_01_08_425864 42 50 palmitate palmitate NNP 10_1101-2021_01_08_425864 42 51 110 110 CD 10_1101-2021_01_08_425864 42 52 spiked spike VBN 10_1101-2021_01_08_425864 42 53 with with IN 10_1101-2021_01_08_425864 42 54 0.2 0.2 CD 10_1101-2021_01_08_425864 42 55 µCi µCi NNS 10_1101-2021_01_08_425864 42 56 of of IN 10_1101-2021_01_08_425864 42 57 14C 14c CD 10_1101-2021_01_08_425864 42 58 - - HYPH 10_1101-2021_01_08_425864 42 59 U u NN 10_1101-2021_01_08_425864 42 60 - - HYPH 10_1101-2021_01_08_425864 42 61 substrates substrate NNS 10_1101-2021_01_08_425864 42 62 . . . 10_1101-2021_01_08_425864 43 1 To to TO 10_1101-2021_01_08_425864 43 2 trap trap VB 10_1101-2021_01_08_425864 43 3 the the DT 10_1101-2021_01_08_425864 43 4 produced produce VBN 10_1101-2021_01_08_425864 43 5 CO2 CO2 NNP 10_1101-2021_01_08_425864 43 6 , , , 10_1101-2021_01_08_425864 43 7 Whatman Whatman NNP 10_1101-2021_01_08_425864 43 8 paper paper NN 10_1101-2021_01_08_425864 43 9 was be VBD 10_1101-2021_01_08_425864 43 10 embedded embed VBN 10_1101-2021_01_08_425864 43 11 111 111 CD 10_1101-2021_01_08_425864 43 12 in in IN 10_1101-2021_01_08_425864 43 13 2 2 CD 10_1101-2021_01_08_425864 43 14 M M NNP 10_1101-2021_01_08_425864 43 15 KOH KOH NNP 10_1101-2021_01_08_425864 43 16 solution solution NN 10_1101-2021_01_08_425864 43 17 and and CC 10_1101-2021_01_08_425864 43 18 was be VBD 10_1101-2021_01_08_425864 43 19 placed place VBN 10_1101-2021_01_08_425864 43 20 in in IN 10_1101-2021_01_08_425864 43 21 the the DT 10_1101-2021_01_08_425864 43 22 top top NN 10_1101-2021_01_08_425864 43 23 of of IN 10_1101-2021_01_08_425864 43 24 the the DT 10_1101-2021_01_08_425864 43 25 tube tube NN 10_1101-2021_01_08_425864 43 26 . . . 10_1101-2021_01_08_425864 44 1 The the DT 10_1101-2021_01_08_425864 44 2 14CO2 14co2 CD 10_1101-2021_01_08_425864 44 3 trapped trap VBN 10_1101-2021_01_08_425864 44 4 by by IN 10_1101-2021_01_08_425864 44 5 this this DT 10_1101-2021_01_08_425864 44 6 reaction reaction NN 10_1101-2021_01_08_425864 44 7 was be VBD 10_1101-2021_01_08_425864 44 8 112 112 CD 10_1101-2021_01_08_425864 44 9 quantified quantify VBN 10_1101-2021_01_08_425864 44 10 by by IN 10_1101-2021_01_08_425864 44 11 scintillation scintillation NN 10_1101-2021_01_08_425864 44 12 [ [ -LRB- 10_1101-2021_01_08_425864 44 13 15,16 15,16 CD 10_1101-2021_01_08_425864 44 14 ] ] -RRB- 10_1101-2021_01_08_425864 44 15 . . . 10_1101-2021_01_08_425864 45 1 113 113 CD 10_1101-2021_01_08_425864 45 2 114 114 CD 10_1101-2021_01_08_425864 45 3 1H 1h CD 10_1101-2021_01_08_425864 45 4 - - HYPH 10_1101-2021_01_08_425864 45 5 NMR nmr JJ 10_1101-2021_01_08_425864 45 6 analysis analysis NN 10_1101-2021_01_08_425864 45 7 of of IN 10_1101-2021_01_08_425864 45 8 the the DT 10_1101-2021_01_08_425864 45 9 exometabolome exometabolome NN 10_1101-2021_01_08_425864 45 10 . . . 10_1101-2021_01_08_425864 46 1 Epimastigotes Epimastigotes NNP 10_1101-2021_01_08_425864 46 2 ( ( -LRB- 10_1101-2021_01_08_425864 46 3 1x108 1x108 CD 10_1101-2021_01_08_425864 46 4 mL-1 mL-1 NNP 10_1101-2021_01_08_425864 46 5 ) ) -RRB- 10_1101-2021_01_08_425864 46 6 were be VBD 10_1101-2021_01_08_425864 46 7 collected collect VBN 10_1101-2021_01_08_425864 46 8 by by IN 10_1101-2021_01_08_425864 46 9 115 115 CD 10_1101-2021_01_08_425864 46 10 centrifugation centrifugation NN 10_1101-2021_01_08_425864 46 11 at at IN 10_1101-2021_01_08_425864 46 12 1,400 1,400 CD 10_1101-2021_01_08_425864 46 13 x x SYM 10_1101-2021_01_08_425864 46 14 g g NNP 10_1101-2021_01_08_425864 46 15 for for IN 10_1101-2021_01_08_425864 46 16 10 10 CD 10_1101-2021_01_08_425864 46 17 min min NN 10_1101-2021_01_08_425864 46 18 , , , 10_1101-2021_01_08_425864 46 19 washed wash VBD 10_1101-2021_01_08_425864 46 20 twice twice RB 10_1101-2021_01_08_425864 46 21 with with IN 10_1101-2021_01_08_425864 46 22 PBS PBS NNP 10_1101-2021_01_08_425864 46 23 and and CC 10_1101-2021_01_08_425864 46 24 incubated incubate VBD 10_1101-2021_01_08_425864 46 25 in in IN 10_1101-2021_01_08_425864 46 26 1 1 CD 10_1101-2021_01_08_425864 46 27 mL mL NNP 10_1101-2021_01_08_425864 46 28 ( ( -LRB- 10_1101-2021_01_08_425864 46 29 single single JJ 10_1101-2021_01_08_425864 46 30 point point NN 10_1101-2021_01_08_425864 46 31 116 116 CD 10_1101-2021_01_08_425864 46 32 analysis analysis NN 10_1101-2021_01_08_425864 46 33 ) ) -RRB- 10_1101-2021_01_08_425864 46 34 of of IN 10_1101-2021_01_08_425864 46 35 PBS PBS NNP 10_1101-2021_01_08_425864 46 36 supplemented supplement VBD 10_1101-2021_01_08_425864 46 37 with with IN 10_1101-2021_01_08_425864 46 38 2 2 CD 10_1101-2021_01_08_425864 46 39 g g NNP 10_1101-2021_01_08_425864 46 40 / / SYM 10_1101-2021_01_08_425864 46 41 L L NNP 10_1101-2021_01_08_425864 46 42 NaHCO3 NaHCO3 NNP 10_1101-2021_01_08_425864 46 43 ( ( -LRB- 10_1101-2021_01_08_425864 46 44 pH pH NNP 10_1101-2021_01_08_425864 46 45 7.4 7.4 CD 10_1101-2021_01_08_425864 46 46 ) ) -RRB- 10_1101-2021_01_08_425864 46 47 . . . 10_1101-2021_01_08_425864 47 1 The the DT 10_1101-2021_01_08_425864 47 2 cells cell NNS 10_1101-2021_01_08_425864 47 3 were be VBD 10_1101-2021_01_08_425864 47 4 maintained maintain VBN 10_1101-2021_01_08_425864 47 5 for for IN 10_1101-2021_01_08_425864 47 6 6 6 CD 10_1101-2021_01_08_425864 47 7 h h NN 10_1101-2021_01_08_425864 47 8 at at IN 10_1101-2021_01_08_425864 47 9 27 27 CD 10_1101-2021_01_08_425864 47 10 117 117 CD 10_1101-2021_01_08_425864 47 11 ° ° NNS 10_1101-2021_01_08_425864 47 12 C c NN 10_1101-2021_01_08_425864 47 13 in in IN 10_1101-2021_01_08_425864 47 14 incubation incubation NN 10_1101-2021_01_08_425864 47 15 buffer buffer NN 10_1101-2021_01_08_425864 47 16 containing contain VBG 10_1101-2021_01_08_425864 47 17 [ [ -LRB- 10_1101-2021_01_08_425864 47 18 U-13C]-glucose u-13c]-glucose IN 10_1101-2021_01_08_425864 47 19 , , , 10_1101-2021_01_08_425864 47 20 non non JJ 10_1101-2021_01_08_425864 47 21 - - JJ 10_1101-2021_01_08_425864 47 22 enriched enriched JJ 10_1101-2021_01_08_425864 47 23 palmitate palmitate NN 10_1101-2021_01_08_425864 47 24 or or CC 10_1101-2021_01_08_425864 47 25 no no DT 10_1101-2021_01_08_425864 47 26 carbon carbon NN 10_1101-2021_01_08_425864 47 27 sources source NNS 10_1101-2021_01_08_425864 47 28 . . . 10_1101-2021_01_08_425864 48 1 The the DT 10_1101-2021_01_08_425864 48 2 118 118 CD 10_1101-2021_01_08_425864 48 3 integrity integrity NN 10_1101-2021_01_08_425864 48 4 of of IN 10_1101-2021_01_08_425864 48 5 the the DT 10_1101-2021_01_08_425864 48 6 cells cell NNS 10_1101-2021_01_08_425864 48 7 during during IN 10_1101-2021_01_08_425864 48 8 the the DT 10_1101-2021_01_08_425864 48 9 incubation incubation NN 10_1101-2021_01_08_425864 48 10 was be VBD 10_1101-2021_01_08_425864 48 11 checked check VBN 10_1101-2021_01_08_425864 48 12 by by IN 10_1101-2021_01_08_425864 48 13 microscopic microscopic JJ 10_1101-2021_01_08_425864 48 14 observation observation NN 10_1101-2021_01_08_425864 48 15 . . . 10_1101-2021_01_08_425864 49 1 The the DT 10_1101-2021_01_08_425864 49 2 supernatant supernatant JJ 10_1101-2021_01_08_425864 49 3 119 119 CD 10_1101-2021_01_08_425864 49 4 ( ( -LRB- 10_1101-2021_01_08_425864 49 5 1 1 CD 10_1101-2021_01_08_425864 49 6 mL mL NNP 10_1101-2021_01_08_425864 49 7 ) ) -RRB- 10_1101-2021_01_08_425864 49 8 was be VBD 10_1101-2021_01_08_425864 49 9 collected collect VBN 10_1101-2021_01_08_425864 49 10 and and CC 10_1101-2021_01_08_425864 49 11 50 50 CD 10_1101-2021_01_08_425864 49 12 µl µl NNS 10_1101-2021_01_08_425864 49 13 of of IN 10_1101-2021_01_08_425864 49 14 maleate maleate NN 10_1101-2021_01_08_425864 49 15 solution solution NN 10_1101-2021_01_08_425864 49 16 in in IN 10_1101-2021_01_08_425864 49 17 D2O d2o NN 10_1101-2021_01_08_425864 49 18 ( ( -LRB- 10_1101-2021_01_08_425864 49 19 10 10 CD 10_1101-2021_01_08_425864 49 20 mM mM NNP 10_1101-2021_01_08_425864 49 21 ) ) -RRB- 10_1101-2021_01_08_425864 49 22 was be VBD 10_1101-2021_01_08_425864 49 23 added add VBN 10_1101-2021_01_08_425864 49 24 as as IN 10_1101-2021_01_08_425864 49 25 an an DT 10_1101-2021_01_08_425864 49 26 internal internal JJ 10_1101-2021_01_08_425864 49 27 120 120 CD 10_1101-2021_01_08_425864 49 28 reference reference NN 10_1101-2021_01_08_425864 49 29 . . . 10_1101-2021_01_08_425864 50 1 1H 1h LS 10_1101-2021_01_08_425864 50 2 - - : 10_1101-2021_01_08_425864 50 3 NMR NMR NNP 10_1101-2021_01_08_425864 50 4 spectra spectra NNP 10_1101-2021_01_08_425864 50 5 were be VBD 10_1101-2021_01_08_425864 50 6 collected collect VBN 10_1101-2021_01_08_425864 50 7 at at IN 10_1101-2021_01_08_425864 50 8 500.19 500.19 CD 10_1101-2021_01_08_425864 50 9 MHz MHz NNS 10_1101-2021_01_08_425864 50 10 on on IN 10_1101-2021_01_08_425864 50 11 a a DT 10_1101-2021_01_08_425864 50 12 Bruker Bruker NNP 10_1101-2021_01_08_425864 50 13 Avance Avance NNP 10_1101-2021_01_08_425864 50 14 III III NNP 10_1101-2021_01_08_425864 50 15 500 500 CD 10_1101-2021_01_08_425864 50 16 HD HD NNP 10_1101-2021_01_08_425864 50 17 121 121 CD 10_1101-2021_01_08_425864 50 18 spectrometer spectrometer VBP 10_1101-2021_01_08_425864 50 19 equipped equip VBN 10_1101-2021_01_08_425864 50 20 with with IN 10_1101-2021_01_08_425864 50 21 a a DT 10_1101-2021_01_08_425864 50 22 5 5 CD 10_1101-2021_01_08_425864 50 23 mm mm NN 10_1101-2021_01_08_425864 50 24 Prodigy Prodigy NNP 10_1101-2021_01_08_425864 50 25 cryoprobe cryoprobe NN 10_1101-2021_01_08_425864 50 26 . . . 10_1101-2021_01_08_425864 51 1 The the DT 10_1101-2021_01_08_425864 51 2 measurements measurement NNS 10_1101-2021_01_08_425864 51 3 were be VBD 10_1101-2021_01_08_425864 51 4 recorded record VBN 10_1101-2021_01_08_425864 51 5 at at IN 10_1101-2021_01_08_425864 51 6 25 25 CD 10_1101-2021_01_08_425864 51 7 ° ° NNS 10_1101-2021_01_08_425864 51 8 C C NNP 10_1101-2021_01_08_425864 51 9 . . . 10_1101-2021_01_08_425864 52 1 122 122 CD 10_1101-2021_01_08_425864 52 2 The the DT 10_1101-2021_01_08_425864 52 3 acquisition acquisition NN 10_1101-2021_01_08_425864 52 4 conditions condition NNS 10_1101-2021_01_08_425864 52 5 were be VBD 10_1101-2021_01_08_425864 52 6 as as IN 10_1101-2021_01_08_425864 52 7 follows follow VBZ 10_1101-2021_01_08_425864 52 8 : : : 10_1101-2021_01_08_425864 52 9 90 90 CD 10_1101-2021_01_08_425864 52 10 ° ° NN 10_1101-2021_01_08_425864 52 11 flip flip JJ 10_1101-2021_01_08_425864 52 12 angle angle NN 10_1101-2021_01_08_425864 52 13 , , , 10_1101-2021_01_08_425864 52 14 5,000 5,000 CD 10_1101-2021_01_08_425864 52 15 Hz Hz NNP 10_1101-2021_01_08_425864 52 16 spectral spectral JJ 10_1101-2021_01_08_425864 52 17 width width NN 10_1101-2021_01_08_425864 52 18 , , , 10_1101-2021_01_08_425864 52 19 32 32 CD 10_1101-2021_01_08_425864 52 20 K k NN 10_1101-2021_01_08_425864 52 21 memory memory NN 10_1101-2021_01_08_425864 52 22 123 123 CD 10_1101-2021_01_08_425864 52 23 size size NN 10_1101-2021_01_08_425864 52 24 , , , 10_1101-2021_01_08_425864 52 25 and and CC 10_1101-2021_01_08_425864 52 26 9.3 9.3 CD 10_1101-2021_01_08_425864 52 27 sec sec NNP 10_1101-2021_01_08_425864 52 28 total total JJ 10_1101-2021_01_08_425864 52 29 recycling recycling NN 10_1101-2021_01_08_425864 52 30 time time NN 10_1101-2021_01_08_425864 52 31 . . . 10_1101-2021_01_08_425864 53 1 The the DT 10_1101-2021_01_08_425864 53 2 measurements measurement NNS 10_1101-2021_01_08_425864 53 3 were be VBD 10_1101-2021_01_08_425864 53 4 performed perform VBN 10_1101-2021_01_08_425864 53 5 with with IN 10_1101-2021_01_08_425864 53 6 64 64 CD 10_1101-2021_01_08_425864 53 7 scans scan NNS 10_1101-2021_01_08_425864 53 8 for for IN 10_1101-2021_01_08_425864 53 9 a a DT 10_1101-2021_01_08_425864 53 10 total total JJ 10_1101-2021_01_08_425864 53 11 124 124 CD 10_1101-2021_01_08_425864 53 12 time time NN 10_1101-2021_01_08_425864 53 13 of of IN 10_1101-2021_01_08_425864 53 14 close close NN 10_1101-2021_01_08_425864 53 15 to to TO 10_1101-2021_01_08_425864 53 16 10 10 CD 10_1101-2021_01_08_425864 53 17 min min NN 10_1101-2021_01_08_425864 53 18 and and CC 10_1101-2021_01_08_425864 53 19 30 30 CD 10_1101-2021_01_08_425864 53 20 sec sec NNP 10_1101-2021_01_08_425864 53 21 . . . 10_1101-2021_01_08_425864 54 1 The the DT 10_1101-2021_01_08_425864 54 2 resonances resonance NNS 10_1101-2021_01_08_425864 54 3 of of IN 10_1101-2021_01_08_425864 54 4 the the DT 10_1101-2021_01_08_425864 54 5 obtained obtained JJ 10_1101-2021_01_08_425864 54 6 spectra spectra NNP 10_1101-2021_01_08_425864 54 7 were be VBD 10_1101-2021_01_08_425864 54 8 integrated integrate VBN 10_1101-2021_01_08_425864 54 9 and and CC 10_1101-2021_01_08_425864 54 10 the the DT 10_1101-2021_01_08_425864 54 11 125 125 CD 10_1101-2021_01_08_425864 54 12 metabolite metabolite JJ 10_1101-2021_01_08_425864 54 13 concentrations concentration NNS 10_1101-2021_01_08_425864 54 14 were be VBD 10_1101-2021_01_08_425864 54 15 calculated calculate VBN 10_1101-2021_01_08_425864 54 16 using use VBG 10_1101-2021_01_08_425864 54 17 the the DT 10_1101-2021_01_08_425864 54 18 ERETIC2 ERETIC2 `` 10_1101-2021_01_08_425864 54 19 NMR NMR NNP 10_1101-2021_01_08_425864 54 20 quantification quantification NN 10_1101-2021_01_08_425864 54 21 Bruker Bruker NNP 10_1101-2021_01_08_425864 54 22 program program NN 10_1101-2021_01_08_425864 54 23 . . . 10_1101-2021_01_08_425864 55 1 126 126 CD 10_1101-2021_01_08_425864 55 2 127 127 CD 10_1101-2021_01_08_425864 55 3 Oxygen oxygen NN 10_1101-2021_01_08_425864 55 4 consumption consumption NN 10_1101-2021_01_08_425864 55 5 . . . 10_1101-2021_01_08_425864 56 1 To to TO 10_1101-2021_01_08_425864 56 2 evaluate evaluate VB 10_1101-2021_01_08_425864 56 3 the the DT 10_1101-2021_01_08_425864 56 4 importance importance NN 10_1101-2021_01_08_425864 56 5 of of IN 10_1101-2021_01_08_425864 56 6 internal internal JJ 10_1101-2021_01_08_425864 56 7 fatty fatty NN 10_1101-2021_01_08_425864 56 8 acid acid NN 10_1101-2021_01_08_425864 56 9 sources source NNS 10_1101-2021_01_08_425864 56 10 in in IN 10_1101-2021_01_08_425864 56 11 O2 o2 CD 10_1101-2021_01_08_425864 56 12 consumption consumption NN 10_1101-2021_01_08_425864 56 13 , , , 10_1101-2021_01_08_425864 56 14 128 128 CD 10_1101-2021_01_08_425864 56 15 exponentially exponentially RB 10_1101-2021_01_08_425864 56 16 growing grow VBG 10_1101-2021_01_08_425864 56 17 parasites parasite NNS 10_1101-2021_01_08_425864 56 18 were be VBD 10_1101-2021_01_08_425864 56 19 treated treat VBN 10_1101-2021_01_08_425864 56 20 or or CC 10_1101-2021_01_08_425864 56 21 not not RB 10_1101-2021_01_08_425864 56 22 treated treat VBN 10_1101-2021_01_08_425864 56 23 with with IN 10_1101-2021_01_08_425864 56 24 500 500 CD 10_1101-2021_01_08_425864 56 25 µM µM NNP 10_1101-2021_01_08_425864 56 26 ETO ETO NNP 10_1101-2021_01_08_425864 56 27 ( ( -LRB- 10_1101-2021_01_08_425864 56 28 the the DT 10_1101-2021_01_08_425864 56 29 inhibitor inhibitor NN 10_1101-2021_01_08_425864 56 30 of of IN 10_1101-2021_01_08_425864 56 31 129 129 CD 10_1101-2021_01_08_425864 56 32 carnitine carnitine NN 10_1101-2021_01_08_425864 56 33 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 56 34 1 1 CD 10_1101-2021_01_08_425864 56 35 ) ) -RRB- 10_1101-2021_01_08_425864 56 36 , , , 10_1101-2021_01_08_425864 56 37 washed wash VBD 10_1101-2021_01_08_425864 56 38 twice twice RB 10_1101-2021_01_08_425864 56 39 in in IN 10_1101-2021_01_08_425864 56 40 PBS PBS NNP 10_1101-2021_01_08_425864 56 41 and and CC 10_1101-2021_01_08_425864 56 42 resuspended resuspend VBD 10_1101-2021_01_08_425864 56 43 in in IN 10_1101-2021_01_08_425864 56 44 Mitochondrial Mitochondrial NNP 10_1101-2021_01_08_425864 56 45 Cellular Cellular NNP 10_1101-2021_01_08_425864 56 46 130 130 CD 10_1101-2021_01_08_425864 56 47 Respiration Respiration NNP 10_1101-2021_01_08_425864 56 48 ( ( -LRB- 10_1101-2021_01_08_425864 56 49 MCR MCR NNP 10_1101-2021_01_08_425864 56 50 ) ) -RRB- 10_1101-2021_01_08_425864 56 51 buffer buffer NN 10_1101-2021_01_08_425864 56 52 . . . 10_1101-2021_01_08_425864 57 1 The the DT 10_1101-2021_01_08_425864 57 2 rates rate NNS 10_1101-2021_01_08_425864 57 3 of of IN 10_1101-2021_01_08_425864 57 4 oxygen oxygen NN 10_1101-2021_01_08_425864 57 5 consumption consumption NN 10_1101-2021_01_08_425864 57 6 were be VBD 10_1101-2021_01_08_425864 57 7 measured measure VBN 10_1101-2021_01_08_425864 57 8 using use VBG 10_1101-2021_01_08_425864 57 9 intact intact JJ 10_1101-2021_01_08_425864 57 10 cells cell NNS 10_1101-2021_01_08_425864 57 11 in in IN 10_1101-2021_01_08_425864 57 12 a a DT 10_1101-2021_01_08_425864 57 13 131 131 CD 10_1101-2021_01_08_425864 57 14 high high JJ 10_1101-2021_01_08_425864 57 15 - - HYPH 10_1101-2021_01_08_425864 57 16 resolution resolution NN 10_1101-2021_01_08_425864 57 17 oxygraph oxygraph NN 10_1101-2021_01_08_425864 57 18 ( ( -LRB- 10_1101-2021_01_08_425864 57 19 Oxygraph-2k oxygraph-2k IN 10_1101-2021_01_08_425864 57 20 ; ; : 10_1101-2021_01_08_425864 57 21 Oroboros Oroboros NNP 10_1101-2021_01_08_425864 57 22 Instruments Instruments NNP 10_1101-2021_01_08_425864 57 23 , , , 10_1101-2021_01_08_425864 57 24 Innsbruck Innsbruck NNP 10_1101-2021_01_08_425864 57 25 , , , 10_1101-2021_01_08_425864 57 26 Austria Austria NNP 10_1101-2021_01_08_425864 57 27 ) ) -RRB- 10_1101-2021_01_08_425864 57 28 . . . 10_1101-2021_01_08_425864 58 1 Oligomycin Oligomycin NNP 10_1101-2021_01_08_425864 58 2 A A NNP 10_1101-2021_01_08_425864 58 3 132 132 CD 10_1101-2021_01_08_425864 58 4 ( ( -LRB- 10_1101-2021_01_08_425864 58 5 0.5 0.5 CD 10_1101-2021_01_08_425864 58 6 µg µg IN 10_1101-2021_01_08_425864 58 7 / / SYM 10_1101-2021_01_08_425864 58 8 mL ml NN 10_1101-2021_01_08_425864 58 9 ) ) -RRB- 10_1101-2021_01_08_425864 58 10 and and CC 10_1101-2021_01_08_425864 58 11 FCCP FCCP NNP 10_1101-2021_01_08_425864 58 12 ( ( -LRB- 10_1101-2021_01_08_425864 58 13 0.5 0.5 CD 10_1101-2021_01_08_425864 58 14 µM µM NNP 10_1101-2021_01_08_425864 58 15 ) ) -RRB- 10_1101-2021_01_08_425864 58 16 were be VBD 10_1101-2021_01_08_425864 58 17 sequentially sequentially RB 10_1101-2021_01_08_425864 58 18 added add VBN 10_1101-2021_01_08_425864 58 19 to to TO 10_1101-2021_01_08_425864 58 20 measure measure VB 10_1101-2021_01_08_425864 58 21 the the DT 10_1101-2021_01_08_425864 58 22 optimal optimal JJ 10_1101-2021_01_08_425864 58 23 non non JJ 10_1101-2021_01_08_425864 58 24 - - JJ 10_1101-2021_01_08_425864 58 25 coupled coupled JJ 10_1101-2021_01_08_425864 58 26 .CC .CC NFP 10_1101-2021_01_08_425864 58 27 - - : 10_1101-2021_01_08_425864 58 28 BY by IN 10_1101-2021_01_08_425864 58 29 4.0 4.0 CD 10_1101-2021_01_08_425864 58 30 International international JJ 10_1101-2021_01_08_425864 58 31 licensemade licensemade NN 10_1101-2021_01_08_425864 58 32 available available JJ 10_1101-2021_01_08_425864 58 33 under under IN 10_1101-2021_01_08_425864 58 34 a a DT 10_1101-2021_01_08_425864 58 35 ( ( -LRB- 10_1101-2021_01_08_425864 58 36 which which WDT 10_1101-2021_01_08_425864 58 37 was be VBD 10_1101-2021_01_08_425864 58 38 not not RB 10_1101-2021_01_08_425864 58 39 certified certify VBN 10_1101-2021_01_08_425864 58 40 by by IN 10_1101-2021_01_08_425864 58 41 peer peer NN 10_1101-2021_01_08_425864 58 42 review review NN 10_1101-2021_01_08_425864 58 43 ) ) -RRB- 10_1101-2021_01_08_425864 58 44 is be VBZ 10_1101-2021_01_08_425864 58 45 the the DT 10_1101-2021_01_08_425864 58 46 author author NN 10_1101-2021_01_08_425864 58 47 / / SYM 10_1101-2021_01_08_425864 58 48 funder funder NN 10_1101-2021_01_08_425864 58 49 , , , 10_1101-2021_01_08_425864 58 50 who who WP 10_1101-2021_01_08_425864 58 51 has have VBZ 10_1101-2021_01_08_425864 58 52 granted grant VBN 10_1101-2021_01_08_425864 58 53 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 58 54 a a DT 10_1101-2021_01_08_425864 58 55 license license NN 10_1101-2021_01_08_425864 58 56 to to TO 10_1101-2021_01_08_425864 58 57 display display VB 10_1101-2021_01_08_425864 58 58 the the DT 10_1101-2021_01_08_425864 58 59 preprint preprint NN 10_1101-2021_01_08_425864 58 60 in in IN 10_1101-2021_01_08_425864 58 61 perpetuity perpetuity NN 10_1101-2021_01_08_425864 58 62 . . . 10_1101-2021_01_08_425864 59 1 It -PRON- PRP 10_1101-2021_01_08_425864 59 2 is be VBZ 10_1101-2021_01_08_425864 59 3 The the DT 10_1101-2021_01_08_425864 59 4 copyright copyright NN 10_1101-2021_01_08_425864 59 5 holder holder NN 10_1101-2021_01_08_425864 59 6 for for IN 10_1101-2021_01_08_425864 59 7 this this DT 10_1101-2021_01_08_425864 59 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 59 9 version version NN 10_1101-2021_01_08_425864 59 10 posted post VBD 10_1101-2021_01_08_425864 59 11 January January NNP 10_1101-2021_01_08_425864 59 12 8 8 CD 10_1101-2021_01_08_425864 59 13 , , , 10_1101-2021_01_08_425864 59 14 2021 2021 CD 10_1101-2021_01_08_425864 59 15 . . . 10_1101-2021_01_08_425864 59 16 ; ; : 10_1101-2021_01_08_425864 59 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 59 18 : : : 10_1101-2021_01_08_425864 59 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 59 20 preprint preprint NN 10_1101-2021_01_08_425864 59 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 59 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 59 23 133 133 CD 10_1101-2021_01_08_425864 59 24 respiration respiration NN 10_1101-2021_01_08_425864 59 25 and and CC 10_1101-2021_01_08_425864 59 26 the the DT 10_1101-2021_01_08_425864 59 27 respiration respiration NN 10_1101-2021_01_08_425864 59 28 leak leak NN 10_1101-2021_01_08_425864 59 29 state state NN 10_1101-2021_01_08_425864 59 30 , , , 10_1101-2021_01_08_425864 59 31 respectively respectively RB 10_1101-2021_01_08_425864 59 32 . . . 10_1101-2021_01_08_425864 60 1 The the DT 10_1101-2021_01_08_425864 60 2 data datum NNS 10_1101-2021_01_08_425864 60 3 were be VBD 10_1101-2021_01_08_425864 60 4 recorded record VBN 10_1101-2021_01_08_425864 60 5 and and CC 10_1101-2021_01_08_425864 60 6 treated treat VBN 10_1101-2021_01_08_425864 60 7 using use VBG 10_1101-2021_01_08_425864 60 8 134 134 CD 10_1101-2021_01_08_425864 60 9 DatLab DatLab NNP 10_1101-2021_01_08_425864 60 10 7 7 CD 10_1101-2021_01_08_425864 60 11 software software NN 10_1101-2021_01_08_425864 60 12 [ [ -LRB- 10_1101-2021_01_08_425864 60 13 15,16,21 15,16,21 NNP 10_1101-2021_01_08_425864 60 14 ] ] -RRB- 10_1101-2021_01_08_425864 60 15 . . . 10_1101-2021_01_08_425864 61 1 135 135 CD 10_1101-2021_01_08_425864 61 2 136 136 CD 10_1101-2021_01_08_425864 61 3 Mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 61 4 activity activity NN 10_1101-2021_01_08_425864 61 5 assays assays RB 10_1101-2021_01_08_425864 61 6 137 137 CD 10_1101-2021_01_08_425864 61 7 MTT MTT NNP 10_1101-2021_01_08_425864 61 8 and and CC 10_1101-2021_01_08_425864 61 9 Alamar Alamar NNP 10_1101-2021_01_08_425864 61 10 Blue Blue NNP 10_1101-2021_01_08_425864 61 11 . . . 10_1101-2021_01_08_425864 62 1 The the DT 10_1101-2021_01_08_425864 62 2 parasites parasite NNS 10_1101-2021_01_08_425864 62 3 were be VBD 10_1101-2021_01_08_425864 62 4 washed wash VBN 10_1101-2021_01_08_425864 62 5 twice twice RB 10_1101-2021_01_08_425864 62 6 and and CC 10_1101-2021_01_08_425864 62 7 incubated incubate VBN 10_1101-2021_01_08_425864 62 8 in in IN 10_1101-2021_01_08_425864 62 9 PBS PBS NNP 10_1101-2021_01_08_425864 62 10 supplemented supplement VBD 10_1101-2021_01_08_425864 62 11 with with IN 10_1101-2021_01_08_425864 62 12 138 138 CD 10_1101-2021_01_08_425864 62 13 0.1 0.1 CD 10_1101-2021_01_08_425864 62 14 mM mm NN 10_1101-2021_01_08_425864 62 15 palmitate palmitate NN 10_1101-2021_01_08_425864 62 16 in in IN 10_1101-2021_01_08_425864 62 17 0.35 0.35 CD 10_1101-2021_01_08_425864 62 18 % % NN 10_1101-2021_01_08_425864 62 19 FFA FFA NNP 10_1101-2021_01_08_425864 62 20 BSA BSA NNP 10_1101-2021_01_08_425864 62 21 , , , 10_1101-2021_01_08_425864 62 22 0.35 0.35 CD 10_1101-2021_01_08_425864 62 23 % % NN 10_1101-2021_01_08_425864 62 24 FFA FFA NNP 10_1101-2021_01_08_425864 62 25 BSA bsa RB 10_1101-2021_01_08_425864 62 26 alone alone JJ 10_1101-2021_01_08_425864 62 27 , , , 10_1101-2021_01_08_425864 62 28 and and CC 10_1101-2021_01_08_425864 62 29 5 5 CD 10_1101-2021_01_08_425864 62 30 mM mm NN 10_1101-2021_01_08_425864 62 31 glucose glucose NN 10_1101-2021_01_08_425864 62 32 , , , 10_1101-2021_01_08_425864 62 33 and and CC 10_1101-2021_01_08_425864 62 34 5 5 CD 10_1101-2021_01_08_425864 62 35 mM mM NNP 10_1101-2021_01_08_425864 62 36 histidine histidine NN 10_1101-2021_01_08_425864 62 37 139 139 CD 10_1101-2021_01_08_425864 62 38 or or CC 10_1101-2021_01_08_425864 62 39 not not RB 10_1101-2021_01_08_425864 62 40 supplemented supplement VBN 10_1101-2021_01_08_425864 62 41 media medium NNS 10_1101-2021_01_08_425864 62 42 were be VBD 10_1101-2021_01_08_425864 62 43 used use VBN 10_1101-2021_01_08_425864 62 44 as as IN 10_1101-2021_01_08_425864 62 45 controls control NNS 10_1101-2021_01_08_425864 62 46 ( ( -LRB- 10_1101-2021_01_08_425864 62 47 positives positive NNS 10_1101-2021_01_08_425864 62 48 and and CC 10_1101-2021_01_08_425864 62 49 negative negative JJ 10_1101-2021_01_08_425864 62 50 , , , 10_1101-2021_01_08_425864 62 51 respectively respectively RB 10_1101-2021_01_08_425864 62 52 ) ) -RRB- 10_1101-2021_01_08_425864 62 53 . . . 10_1101-2021_01_08_425864 63 1 The the DT 10_1101-2021_01_08_425864 63 2 cell cell NN 10_1101-2021_01_08_425864 63 3 140 140 CD 10_1101-2021_01_08_425864 63 4 viability viability NN 10_1101-2021_01_08_425864 63 5 was be VBD 10_1101-2021_01_08_425864 63 6 evaluated evaluate VBN 10_1101-2021_01_08_425864 63 7 at at IN 10_1101-2021_01_08_425864 63 8 24 24 CD 10_1101-2021_01_08_425864 63 9 h h NN 10_1101-2021_01_08_425864 63 10 and and CC 10_1101-2021_01_08_425864 63 11 48 48 CD 10_1101-2021_01_08_425864 63 12 h h NN 10_1101-2021_01_08_425864 63 13 after after IN 10_1101-2021_01_08_425864 63 14 incubation incubation NN 10_1101-2021_01_08_425864 63 15 using use VBG 10_1101-2021_01_08_425864 63 16 the the DT 10_1101-2021_01_08_425864 63 17 MTT MTT NNP 10_1101-2021_01_08_425864 63 18 assay assay NN 10_1101-2021_01_08_425864 63 19 , , , 10_1101-2021_01_08_425864 63 20 as as IN 10_1101-2021_01_08_425864 63 21 described describe VBN 10_1101-2021_01_08_425864 63 22 in in IN 10_1101-2021_01_08_425864 63 23 [ [ -LRB- 10_1101-2021_01_08_425864 63 24 15,16 15,16 CD 10_1101-2021_01_08_425864 63 25 ] ] -RRB- 10_1101-2021_01_08_425864 63 26 . . . 10_1101-2021_01_08_425864 64 1 141 141 CD 10_1101-2021_01_08_425864 64 2 Alamar Alamar NNP 10_1101-2021_01_08_425864 64 3 Blue Blue NNP 10_1101-2021_01_08_425864 64 4 . . . 10_1101-2021_01_08_425864 65 1 The the DT 10_1101-2021_01_08_425864 65 2 parasites parasite NNS 10_1101-2021_01_08_425864 65 3 were be VBD 10_1101-2021_01_08_425864 65 4 washed wash VBN 10_1101-2021_01_08_425864 65 5 twice twice RB 10_1101-2021_01_08_425864 65 6 and and CC 10_1101-2021_01_08_425864 65 7 incubated incubate VBN 10_1101-2021_01_08_425864 65 8 in in IN 10_1101-2021_01_08_425864 65 9 PBS PBS NNP 10_1101-2021_01_08_425864 65 10 or or CC 10_1101-2021_01_08_425864 65 11 PBS PBS NNP 10_1101-2021_01_08_425864 65 12 supplemented supplement VBD 10_1101-2021_01_08_425864 65 13 with with IN 10_1101-2021_01_08_425864 65 14 500 500 CD 10_1101-2021_01_08_425864 65 15 142 142 CD 10_1101-2021_01_08_425864 65 16 μM μM NNP 10_1101-2021_01_08_425864 65 17 ETO eto NN 10_1101-2021_01_08_425864 65 18 in in IN 10_1101-2021_01_08_425864 65 19 96-well 96-well CD 10_1101-2021_01_08_425864 65 20 plates plate NNS 10_1101-2021_01_08_425864 65 21 . . . 10_1101-2021_01_08_425864 66 1 The the DT 10_1101-2021_01_08_425864 66 2 plates plate NNS 10_1101-2021_01_08_425864 66 3 were be VBD 10_1101-2021_01_08_425864 66 4 maintained maintain VBN 10_1101-2021_01_08_425864 66 5 at at IN 10_1101-2021_01_08_425864 66 6 28 28 CD 10_1101-2021_01_08_425864 66 7 ° ° , 10_1101-2021_01_08_425864 66 8 C c NN 10_1101-2021_01_08_425864 66 9 during during IN 10_1101-2021_01_08_425864 66 10 all all PDT 10_1101-2021_01_08_425864 66 11 the the DT 10_1101-2021_01_08_425864 66 12 experiments experiment NNS 10_1101-2021_01_08_425864 66 13 . . . 10_1101-2021_01_08_425864 67 1 After after IN 10_1101-2021_01_08_425864 67 2 143 143 CD 10_1101-2021_01_08_425864 67 3 every every DT 10_1101-2021_01_08_425864 67 4 24 24 CD 10_1101-2021_01_08_425864 67 5 h h NN 10_1101-2021_01_08_425864 67 6 , , , 10_1101-2021_01_08_425864 67 7 the the DT 10_1101-2021_01_08_425864 67 8 cells cell NNS 10_1101-2021_01_08_425864 67 9 were be VBD 10_1101-2021_01_08_425864 67 10 incubated incubate VBN 10_1101-2021_01_08_425864 67 11 with with IN 10_1101-2021_01_08_425864 67 12 0.125 0.125 CD 10_1101-2021_01_08_425864 67 13 μg.mL-1 μg.mL-1 NNS 10_1101-2021_01_08_425864 67 14 of of IN 10_1101-2021_01_08_425864 67 15 Alamar Alamar NNP 10_1101-2021_01_08_425864 67 16 Blue blue JJ 10_1101-2021_01_08_425864 67 17 reagent reagent NN 10_1101-2021_01_08_425864 67 18 and and CC 10_1101-2021_01_08_425864 67 19 kept keep VBD 10_1101-2021_01_08_425864 67 20 at at IN 10_1101-2021_01_08_425864 67 21 28 28 CD 10_1101-2021_01_08_425864 67 22 ° ° , 10_1101-2021_01_08_425864 67 23 C C NNP 10_1101-2021_01_08_425864 67 24 for for IN 10_1101-2021_01_08_425864 67 25 144 144 CD 10_1101-2021_01_08_425864 67 26 2 2 CD 10_1101-2021_01_08_425864 67 27 h h NN 10_1101-2021_01_08_425864 67 28 under under IN 10_1101-2021_01_08_425864 67 29 protection protection NN 10_1101-2021_01_08_425864 67 30 from from IN 10_1101-2021_01_08_425864 67 31 light light NN 10_1101-2021_01_08_425864 67 32 . . . 10_1101-2021_01_08_425864 68 1 The the DT 10_1101-2021_01_08_425864 68 2 fluorescence fluorescence NN 10_1101-2021_01_08_425864 68 3 was be VBD 10_1101-2021_01_08_425864 68 4 accessed access VBN 10_1101-2021_01_08_425864 68 5 using use VBG 10_1101-2021_01_08_425864 68 6 the the DT 10_1101-2021_01_08_425864 68 7 wavelengths wavelength NNS 10_1101-2021_01_08_425864 68 8 λexc λexc VB 10_1101-2021_01_08_425864 68 9 = = SYM 10_1101-2021_01_08_425864 68 10 530 530 CD 10_1101-2021_01_08_425864 68 11 nm nm CC 10_1101-2021_01_08_425864 68 12 145 145 CD 10_1101-2021_01_08_425864 68 13 and and CC 10_1101-2021_01_08_425864 68 14 λem λem NN 10_1101-2021_01_08_425864 68 15 = = SYM 10_1101-2021_01_08_425864 68 16 590 590 CD 10_1101-2021_01_08_425864 68 17 nm nm UH 10_1101-2021_01_08_425864 68 18 in in IN 10_1101-2021_01_08_425864 68 19 the the DT 10_1101-2021_01_08_425864 68 20 SpectraMax SpectraMax NNP 10_1101-2021_01_08_425864 68 21 ® ® NNPS 10_1101-2021_01_08_425864 68 22 i3 i3 NNP 10_1101-2021_01_08_425864 68 23 ( ( -LRB- 10_1101-2021_01_08_425864 68 24 Molecular Molecular NNP 10_1101-2021_01_08_425864 68 25 Devices Devices NNPS 10_1101-2021_01_08_425864 68 26 ) ) -RRB- 10_1101-2021_01_08_425864 68 27 plate plate VBP 10_1101-2021_01_08_425864 68 28 reader reader NN 10_1101-2021_01_08_425864 68 29 . . . 10_1101-2021_01_08_425864 69 1 146 146 CD 10_1101-2021_01_08_425864 69 2 147 147 CD 10_1101-2021_01_08_425864 69 3 Measurement Measurement NNP 10_1101-2021_01_08_425864 69 4 of of IN 10_1101-2021_01_08_425864 69 5 intracellular intracellular JJ 10_1101-2021_01_08_425864 69 6 ATP ATP NNP 10_1101-2021_01_08_425864 69 7 content content NN 10_1101-2021_01_08_425864 69 8 148 148 CD 10_1101-2021_01_08_425864 69 9 The the DT 10_1101-2021_01_08_425864 69 10 intracellular intracellular JJ 10_1101-2021_01_08_425864 69 11 ATP atp NN 10_1101-2021_01_08_425864 69 12 levels level NNS 10_1101-2021_01_08_425864 69 13 were be VBD 10_1101-2021_01_08_425864 69 14 assessed assess VBN 10_1101-2021_01_08_425864 69 15 using use VBG 10_1101-2021_01_08_425864 69 16 a a DT 10_1101-2021_01_08_425864 69 17 luciferase luciferase NN 10_1101-2021_01_08_425864 69 18 assay assay NN 10_1101-2021_01_08_425864 69 19 kit kit NN 10_1101-2021_01_08_425864 69 20 ( ( -LRB- 10_1101-2021_01_08_425864 69 21 Sigma Sigma NNP 10_1101-2021_01_08_425864 69 22 - - HYPH 10_1101-2021_01_08_425864 69 23 Aldrich Aldrich NNP 10_1101-2021_01_08_425864 69 24 ® ® NNP 10_1101-2021_01_08_425864 69 25 ) ) -RRB- 10_1101-2021_01_08_425864 69 26 , , , 10_1101-2021_01_08_425864 69 27 as as IN 10_1101-2021_01_08_425864 69 28 149 149 CD 10_1101-2021_01_08_425864 69 29 described describe VBN 10_1101-2021_01_08_425864 69 30 in in IN 10_1101-2021_01_08_425864 69 31 [ [ -LRB- 10_1101-2021_01_08_425864 69 32 15–17 15–17 CD 10_1101-2021_01_08_425864 69 33 ] ] -RRB- 10_1101-2021_01_08_425864 69 34 . . . 10_1101-2021_01_08_425864 70 1 In in IN 10_1101-2021_01_08_425864 70 2 brief brief NN 10_1101-2021_01_08_425864 70 3 , , , 10_1101-2021_01_08_425864 70 4 the the DT 10_1101-2021_01_08_425864 70 5 parasites parasite NNS 10_1101-2021_01_08_425864 70 6 were be VBD 10_1101-2021_01_08_425864 70 7 incubated incubate VBN 10_1101-2021_01_08_425864 70 8 in in IN 10_1101-2021_01_08_425864 70 9 PBS PBS NNP 10_1101-2021_01_08_425864 70 10 supplemented supplement VBN 10_1101-2021_01_08_425864 70 11 ( ( -LRB- 10_1101-2021_01_08_425864 70 12 or or CC 10_1101-2021_01_08_425864 70 13 not not RB 10_1101-2021_01_08_425864 70 14 ) ) -RRB- 10_1101-2021_01_08_425864 70 15 with with IN 10_1101-2021_01_08_425864 70 16 0.1 0.1 CD 10_1101-2021_01_08_425864 70 17 150 150 CD 10_1101-2021_01_08_425864 70 18 mM mm NN 10_1101-2021_01_08_425864 70 19 palmitate palmitate NN 10_1101-2021_01_08_425864 70 20 , , , 10_1101-2021_01_08_425864 70 21 0.35 0.35 CD 10_1101-2021_01_08_425864 70 22 % % NN 10_1101-2021_01_08_425864 70 23 FFA FFA NNP 10_1101-2021_01_08_425864 70 24 BSA BSA NNP 10_1101-2021_01_08_425864 70 25 , , , 10_1101-2021_01_08_425864 70 26 5 5 CD 10_1101-2021_01_08_425864 70 27 mM mm NN 10_1101-2021_01_08_425864 70 28 glucose glucose NN 10_1101-2021_01_08_425864 70 29 or or CC 10_1101-2021_01_08_425864 70 30 5 5 CD 10_1101-2021_01_08_425864 70 31 mM mM NNP 10_1101-2021_01_08_425864 70 32 histidine histidine NN 10_1101-2021_01_08_425864 70 33 for for IN 10_1101-2021_01_08_425864 70 34 24 24 CD 10_1101-2021_01_08_425864 70 35 h h NN 10_1101-2021_01_08_425864 70 36 at at IN 10_1101-2021_01_08_425864 70 37 28 28 CD 10_1101-2021_01_08_425864 70 38 ° ° , 10_1101-2021_01_08_425864 70 39 C C NNP 10_1101-2021_01_08_425864 70 40 . . . 10_1101-2021_01_08_425864 71 1 The the DT 10_1101-2021_01_08_425864 71 2 ATP ATP NNP 10_1101-2021_01_08_425864 71 3 151 151 CD 10_1101-2021_01_08_425864 71 4 concentrations concentration NNS 10_1101-2021_01_08_425864 71 5 were be VBD 10_1101-2021_01_08_425864 71 6 determined determine VBN 10_1101-2021_01_08_425864 71 7 by by IN 10_1101-2021_01_08_425864 71 8 using use VBG 10_1101-2021_01_08_425864 71 9 a a DT 10_1101-2021_01_08_425864 71 10 calibration calibration NN 10_1101-2021_01_08_425864 71 11 curve curve NN 10_1101-2021_01_08_425864 71 12 with with IN 10_1101-2021_01_08_425864 71 13 ATP atp NN 10_1101-2021_01_08_425864 71 14 disodium disodium NN 10_1101-2021_01_08_425864 71 15 salt salt NN 10_1101-2021_01_08_425864 71 16 ( ( -LRB- 10_1101-2021_01_08_425864 71 17 Sigma Sigma NNP 10_1101-2021_01_08_425864 71 18 ) ) -RRB- 10_1101-2021_01_08_425864 71 19 , , , 10_1101-2021_01_08_425864 71 20 and and CC 10_1101-2021_01_08_425864 71 21 152 152 CD 10_1101-2021_01_08_425864 71 22 the the DT 10_1101-2021_01_08_425864 71 23 luminescence luminescence NN 10_1101-2021_01_08_425864 71 24 at at IN 10_1101-2021_01_08_425864 71 25 570 570 CD 10_1101-2021_01_08_425864 71 26 nm nm CD 10_1101-2021_01_08_425864 71 27 was be VBD 10_1101-2021_01_08_425864 71 28 measured measure VBN 10_1101-2021_01_08_425864 71 29 as as IN 10_1101-2021_01_08_425864 71 30 indicated indicate VBN 10_1101-2021_01_08_425864 71 31 by by IN 10_1101-2021_01_08_425864 71 32 the the DT 10_1101-2021_01_08_425864 71 33 manufacturer manufacturer NN 10_1101-2021_01_08_425864 71 34 . . . 10_1101-2021_01_08_425864 72 1 153 153 CD 10_1101-2021_01_08_425864 72 2 154 154 CD 10_1101-2021_01_08_425864 72 3 Enzymatic enzymatic JJ 10_1101-2021_01_08_425864 72 4 activities activity NNS 10_1101-2021_01_08_425864 72 5 155 155 CD 10_1101-2021_01_08_425864 72 6 Carnitine Carnitine NNP 10_1101-2021_01_08_425864 72 7 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 72 8 1 1 CD 10_1101-2021_01_08_425864 72 9 ( ( -LRB- 10_1101-2021_01_08_425864 72 10 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 72 11 ) ) -RRB- 10_1101-2021_01_08_425864 72 12 . . . 10_1101-2021_01_08_425864 73 1 The the DT 10_1101-2021_01_08_425864 73 2 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 73 3 were be VBD 10_1101-2021_01_08_425864 73 4 washed wash VBN 10_1101-2021_01_08_425864 73 5 twice twice RB 10_1101-2021_01_08_425864 73 6 in in IN 10_1101-2021_01_08_425864 73 7 PBS PBS NNP 10_1101-2021_01_08_425864 73 8 ( ( -LRB- 10_1101-2021_01_08_425864 73 9 1,000 1,000 CD 10_1101-2021_01_08_425864 73 10 x x SYM 10_1101-2021_01_08_425864 73 11 156 156 CD 10_1101-2021_01_08_425864 73 12 g g NN 10_1101-2021_01_08_425864 73 13 , , , 10_1101-2021_01_08_425864 73 14 5 5 CD 10_1101-2021_01_08_425864 73 15 min min NN 10_1101-2021_01_08_425864 73 16 at at IN 10_1101-2021_01_08_425864 73 17 4 4 CD 10_1101-2021_01_08_425864 73 18 ° ° , 10_1101-2021_01_08_425864 73 19 C C NNP 10_1101-2021_01_08_425864 73 20 ) ) -RRB- 10_1101-2021_01_08_425864 73 21 , , , 10_1101-2021_01_08_425864 73 22 resuspended resuspend VBN 10_1101-2021_01_08_425864 73 23 in in IN 10_1101-2021_01_08_425864 73 24 buffered buffered JJ 10_1101-2021_01_08_425864 73 25 Tris Tris NNP 10_1101-2021_01_08_425864 73 26 - - HYPH 10_1101-2021_01_08_425864 73 27 EDTA EDTA NNP 10_1101-2021_01_08_425864 73 28 ( ( -LRB- 10_1101-2021_01_08_425864 73 29 100 100 CD 10_1101-2021_01_08_425864 73 30 mM mm NN 10_1101-2021_01_08_425864 73 31 , , , 10_1101-2021_01_08_425864 73 32 2.5 2.5 CD 10_1101-2021_01_08_425864 73 33 mM mM NNP 10_1101-2021_01_08_425864 73 34 and and CC 10_1101-2021_01_08_425864 73 35 0.1 0.1 CD 10_1101-2021_01_08_425864 73 36 % % NN 10_1101-2021_01_08_425864 73 37 Triton Triton NNP 10_1101-2021_01_08_425864 73 38 X-100 X-100 NNP 10_1101-2021_01_08_425864 73 39 ) ) -RRB- 10_1101-2021_01_08_425864 73 40 157 157 CD 10_1101-2021_01_08_425864 73 41 containing contain VBG 10_1101-2021_01_08_425864 73 42 1 1 CD 10_1101-2021_01_08_425864 73 43 µM µM NNP 10_1101-2021_01_08_425864 73 44 phenylmethyl phenylmethyl NN 10_1101-2021_01_08_425864 73 45 - - HYPH 10_1101-2021_01_08_425864 73 46 sulphonyl sulphonyl NN 10_1101-2021_01_08_425864 73 47 fluoride fluoride NN 10_1101-2021_01_08_425864 73 48 ( ( -LRB- 10_1101-2021_01_08_425864 73 49 PMSF PMSF NNP 10_1101-2021_01_08_425864 73 50 ) ) -RRB- 10_1101-2021_01_08_425864 73 51 , , , 10_1101-2021_01_08_425864 73 52 0.5 0.5 CD 10_1101-2021_01_08_425864 73 53 mM mm NN 10_1101-2021_01_08_425864 73 54 N n CD 10_1101-2021_01_08_425864 73 55 - - HYPH 10_1101-2021_01_08_425864 73 56 alpha alpha NN 10_1101-2021_01_08_425864 73 57 - - HYPH 10_1101-2021_01_08_425864 73 58 p p NN 10_1101-2021_01_08_425864 73 59 - - HYPH 10_1101-2021_01_08_425864 73 60 tosyl tosyl NN 10_1101-2021_01_08_425864 73 61 - - HYPH 10_1101-2021_01_08_425864 73 62 lysyl- lysyl- NN 10_1101-2021_01_08_425864 73 63 158 158 CD 10_1101-2021_01_08_425864 73 64 chloromethyl chloromethyl NN 10_1101-2021_01_08_425864 73 65 ketone ketone NN 10_1101-2021_01_08_425864 73 66 ( ( -LRB- 10_1101-2021_01_08_425864 73 67 TLCK TLCK NNP 10_1101-2021_01_08_425864 73 68 ) ) -RRB- 10_1101-2021_01_08_425864 73 69 , , , 10_1101-2021_01_08_425864 73 70 0.01 0.01 CD 10_1101-2021_01_08_425864 73 71 mg mg NNP 10_1101-2021_01_08_425864 73 72 aprotinin aprotinin NN 10_1101-2021_01_08_425864 73 73 and and CC 10_1101-2021_01_08_425864 73 74 0.1 0.1 CD 10_1101-2021_01_08_425864 73 75 mM mM NNP 10_1101-2021_01_08_425864 73 76 trans trans JJ 10_1101-2021_01_08_425864 73 77 - - JJ 10_1101-2021_01_08_425864 73 78 epoxysuccinyl epoxysuccinyl JJ 10_1101-2021_01_08_425864 73 79 - - HYPH 10_1101-2021_01_08_425864 73 80 L l NN 10_1101-2021_01_08_425864 73 81 - - HYPH 10_1101-2021_01_08_425864 73 82 leucyl leucyl NNP 10_1101-2021_01_08_425864 73 83 amido amido NNP 10_1101-2021_01_08_425864 73 84 159 159 CD 10_1101-2021_01_08_425864 73 85 ( ( -LRB- 10_1101-2021_01_08_425864 73 86 4-guanidino 4-guanidino CD 10_1101-2021_01_08_425864 73 87 ) ) -RRB- 10_1101-2021_01_08_425864 73 88 butane butane NN 10_1101-2021_01_08_425864 73 89 ( ( -LRB- 10_1101-2021_01_08_425864 73 90 E-64 e-64 CD 10_1101-2021_01_08_425864 73 91 ) ) -RRB- 10_1101-2021_01_08_425864 73 92 as as IN 10_1101-2021_01_08_425864 73 93 a a DT 10_1101-2021_01_08_425864 73 94 protease protease NN 10_1101-2021_01_08_425864 73 95 inhibitors inhibitor NNS 10_1101-2021_01_08_425864 73 96 ( ( -LRB- 10_1101-2021_01_08_425864 73 97 Sigma Sigma NNP 10_1101-2021_01_08_425864 73 98 Aldrich Aldrich NNP 10_1101-2021_01_08_425864 73 99 ® ® NNP 10_1101-2021_01_08_425864 73 100 ) ) -RRB- 10_1101-2021_01_08_425864 73 101 and and CC 10_1101-2021_01_08_425864 73 102 lysed lyse VBN 10_1101-2021_01_08_425864 73 103 by by IN 10_1101-2021_01_08_425864 73 104 sonication sonication NN 10_1101-2021_01_08_425864 73 105 ( ( -LRB- 10_1101-2021_01_08_425864 73 106 5 5 CD 10_1101-2021_01_08_425864 73 107 160 160 CD 10_1101-2021_01_08_425864 73 108 pulses pulse NNS 10_1101-2021_01_08_425864 73 109 for for IN 10_1101-2021_01_08_425864 73 110 1 1 CD 10_1101-2021_01_08_425864 73 111 min min NN 10_1101-2021_01_08_425864 73 112 each each DT 10_1101-2021_01_08_425864 73 113 , , , 10_1101-2021_01_08_425864 73 114 20 20 CD 10_1101-2021_01_08_425864 73 115 % % NN 10_1101-2021_01_08_425864 73 116 ) ) -RRB- 10_1101-2021_01_08_425864 73 117 . . . 10_1101-2021_01_08_425864 74 1 The the DT 10_1101-2021_01_08_425864 74 2 lysates lysate NNS 10_1101-2021_01_08_425864 74 3 were be VBD 10_1101-2021_01_08_425864 74 4 clarified clarify VBN 10_1101-2021_01_08_425864 74 5 by by IN 10_1101-2021_01_08_425864 74 6 centrifugation centrifugation NN 10_1101-2021_01_08_425864 74 7 at at IN 10_1101-2021_01_08_425864 74 8 10,000 10,000 CD 10_1101-2021_01_08_425864 74 9 x x SYM 10_1101-2021_01_08_425864 74 10 g g NN 10_1101-2021_01_08_425864 74 11 for for IN 10_1101-2021_01_08_425864 74 12 30 30 CD 10_1101-2021_01_08_425864 74 13 min min NN 10_1101-2021_01_08_425864 74 14 at at IN 10_1101-2021_01_08_425864 74 15 161 161 CD 10_1101-2021_01_08_425864 74 16 4 4 CD 10_1101-2021_01_08_425864 74 17 ° ° , 10_1101-2021_01_08_425864 74 18 C C NNP 10_1101-2021_01_08_425864 74 19 . . . 10_1101-2021_01_08_425864 75 1 The the DT 10_1101-2021_01_08_425864 75 2 soluble soluble JJ 10_1101-2021_01_08_425864 75 3 fraction fraction NN 10_1101-2021_01_08_425864 75 4 was be VBD 10_1101-2021_01_08_425864 75 5 collected collect VBN 10_1101-2021_01_08_425864 75 6 and and CC 10_1101-2021_01_08_425864 75 7 the the DT 10_1101-2021_01_08_425864 75 8 proteins protein NNS 10_1101-2021_01_08_425864 75 9 were be VBD 10_1101-2021_01_08_425864 75 10 quantified quantify VBN 10_1101-2021_01_08_425864 75 11 by by IN 10_1101-2021_01_08_425864 75 12 Bradford Bradford NNP 10_1101-2021_01_08_425864 75 13 method method NN 10_1101-2021_01_08_425864 75 14 [ [ -LRB- 10_1101-2021_01_08_425864 75 15 22 22 CD 10_1101-2021_01_08_425864 75 16 ] ] -RRB- 10_1101-2021_01_08_425864 75 17 162 162 CD 10_1101-2021_01_08_425864 75 18 and and CC 10_1101-2021_01_08_425864 75 19 adjusted adjust VBN 10_1101-2021_01_08_425864 75 20 to to IN 10_1101-2021_01_08_425864 75 21 0.1 0.1 CD 10_1101-2021_01_08_425864 75 22 mg mg NNP 10_1101-2021_01_08_425864 75 23 / / SYM 10_1101-2021_01_08_425864 75 24 mL mL NNP 10_1101-2021_01_08_425864 75 25 protein protein NN 10_1101-2021_01_08_425864 75 26 . . . 10_1101-2021_01_08_425864 76 1 The the DT 10_1101-2021_01_08_425864 76 2 reaction reaction NN 10_1101-2021_01_08_425864 76 3 mixture mixture NN 10_1101-2021_01_08_425864 76 4 contained contain VBD 10_1101-2021_01_08_425864 76 5 0.5 0.5 CD 10_1101-2021_01_08_425864 76 6 mM mM NNP 10_1101-2021_01_08_425864 76 7 L L NNP 10_1101-2021_01_08_425864 76 8 - - HYPH 10_1101-2021_01_08_425864 76 9 carnitine carnitine NN 10_1101-2021_01_08_425864 76 10 , , , 10_1101-2021_01_08_425864 76 11 0.1 0.1 CD 10_1101-2021_01_08_425864 76 12 mM mM NNP 10_1101-2021_01_08_425864 76 13 163 163 CD 10_1101-2021_01_08_425864 76 14 palmitoyl palmitoyl NN 10_1101-2021_01_08_425864 76 15 - - HYPH 10_1101-2021_01_08_425864 76 16 CoA coa NN 10_1101-2021_01_08_425864 76 17 and and CC 10_1101-2021_01_08_425864 76 18 2.5 2.5 CD 10_1101-2021_01_08_425864 76 19 mM mM NNP 10_1101-2021_01_08_425864 76 20 DTNB dtnb NN 10_1101-2021_01_08_425864 76 21 in in IN 10_1101-2021_01_08_425864 76 22 Tris Tris NNP 10_1101-2021_01_08_425864 76 23 - - HYPH 10_1101-2021_01_08_425864 76 24 EDTA EDTA NNP 10_1101-2021_01_08_425864 76 25 buffer buffer NN 10_1101-2021_01_08_425864 76 26 ( ( -LRB- 10_1101-2021_01_08_425864 76 27 pH pH NNP 10_1101-2021_01_08_425864 76 28 = = SYM 10_1101-2021_01_08_425864 76 29 8.0 8.0 CD 10_1101-2021_01_08_425864 76 30 ) ) -RRB- 10_1101-2021_01_08_425864 76 31 . . . 10_1101-2021_01_08_425864 77 1 The the DT 10_1101-2021_01_08_425864 77 2 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 77 3 activity activity NN 10_1101-2021_01_08_425864 77 4 was be VBD 10_1101-2021_01_08_425864 77 5 measured measure VBN 10_1101-2021_01_08_425864 77 6 164 164 CD 10_1101-2021_01_08_425864 77 7 spectrophotometrically spectrophotometrically RB 10_1101-2021_01_08_425864 77 8 at at IN 10_1101-2021_01_08_425864 77 9 412 412 CD 10_1101-2021_01_08_425864 77 10 nm nm NN 10_1101-2021_01_08_425864 77 11 by by IN 10_1101-2021_01_08_425864 77 12 DTNB dtnb NN 10_1101-2021_01_08_425864 77 13 reaction reaction NN 10_1101-2021_01_08_425864 77 14 with with IN 10_1101-2021_01_08_425864 77 15 free free JJ 10_1101-2021_01_08_425864 77 16 HS HS NNP 10_1101-2021_01_08_425864 77 17 - - HYPH 10_1101-2021_01_08_425864 77 18 CoA CoA NNP 10_1101-2021_01_08_425864 77 19 , , , 10_1101-2021_01_08_425864 77 20 forming form VBG 10_1101-2021_01_08_425864 77 21 the the DT 10_1101-2021_01_08_425864 77 22 TNB- TNB- NNP 10_1101-2021_01_08_425864 77 23 ion ion NN 10_1101-2021_01_08_425864 77 24 . . . 10_1101-2021_01_08_425864 78 1 To to IN 10_1101-2021_01_08_425864 78 2 165 165 CD 10_1101-2021_01_08_425864 78 3 calculate calculate NN 10_1101-2021_01_08_425864 78 4 the the DT 10_1101-2021_01_08_425864 78 5 specific specific JJ 10_1101-2021_01_08_425864 78 6 activity activity NN 10_1101-2021_01_08_425864 78 7 , , , 10_1101-2021_01_08_425864 78 8 the the DT 10_1101-2021_01_08_425864 78 9 absorbance absorbance NN 10_1101-2021_01_08_425864 78 10 values value NNS 10_1101-2021_01_08_425864 78 11 were be VBD 10_1101-2021_01_08_425864 78 12 converted convert VBN 10_1101-2021_01_08_425864 78 13 into into IN 10_1101-2021_01_08_425864 78 14 molarity molarity NN 10_1101-2021_01_08_425864 78 15 by by IN 10_1101-2021_01_08_425864 78 16 using use VBG 10_1101-2021_01_08_425864 78 17 the the DT 10_1101-2021_01_08_425864 78 18 TNB- TNB- NNP 10_1101-2021_01_08_425864 78 19 166 166 CD 10_1101-2021_01_08_425864 78 20 extinction extinction NN 10_1101-2021_01_08_425864 78 21 molar molar JJ 10_1101-2021_01_08_425864 78 22 coefficient coefficient NN 10_1101-2021_01_08_425864 78 23 of of IN 10_1101-2021_01_08_425864 78 24 12,000 12,000 CD 10_1101-2021_01_08_425864 78 25 M-1.s-1 M-1.s-1 NNS 10_1101-2021_01_08_425864 78 26 [ [ -LRB- 10_1101-2021_01_08_425864 78 27 23 23 CD 10_1101-2021_01_08_425864 78 28 ] ] -RRB- 10_1101-2021_01_08_425864 78 29 . . . 10_1101-2021_01_08_425864 79 1 As as IN 10_1101-2021_01_08_425864 79 2 a a DT 10_1101-2021_01_08_425864 79 3 blank blank NN 10_1101-2021_01_08_425864 79 4 , , , 10_1101-2021_01_08_425864 79 5 we -PRON- PRP 10_1101-2021_01_08_425864 79 6 performed perform VBD 10_1101-2021_01_08_425864 79 7 the the DT 10_1101-2021_01_08_425864 79 8 same same JJ 10_1101-2021_01_08_425864 79 9 assay assay NN 10_1101-2021_01_08_425864 79 10 without without IN 10_1101-2021_01_08_425864 79 11 .CC .CC : 10_1101-2021_01_08_425864 79 12 - - : 10_1101-2021_01_08_425864 79 13 BY by IN 10_1101-2021_01_08_425864 79 14 4.0 4.0 CD 10_1101-2021_01_08_425864 79 15 International international JJ 10_1101-2021_01_08_425864 79 16 licensemade licensemade NN 10_1101-2021_01_08_425864 79 17 available available JJ 10_1101-2021_01_08_425864 79 18 under under IN 10_1101-2021_01_08_425864 79 19 a a DT 10_1101-2021_01_08_425864 79 20 ( ( -LRB- 10_1101-2021_01_08_425864 79 21 which which WDT 10_1101-2021_01_08_425864 79 22 was be VBD 10_1101-2021_01_08_425864 79 23 not not RB 10_1101-2021_01_08_425864 79 24 certified certify VBN 10_1101-2021_01_08_425864 79 25 by by IN 10_1101-2021_01_08_425864 79 26 peer peer NN 10_1101-2021_01_08_425864 79 27 review review NN 10_1101-2021_01_08_425864 79 28 ) ) -RRB- 10_1101-2021_01_08_425864 79 29 is be VBZ 10_1101-2021_01_08_425864 79 30 the the DT 10_1101-2021_01_08_425864 79 31 author author NN 10_1101-2021_01_08_425864 79 32 / / SYM 10_1101-2021_01_08_425864 79 33 funder funder NN 10_1101-2021_01_08_425864 79 34 , , , 10_1101-2021_01_08_425864 79 35 who who WP 10_1101-2021_01_08_425864 79 36 has have VBZ 10_1101-2021_01_08_425864 79 37 granted grant VBN 10_1101-2021_01_08_425864 79 38 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 79 39 a a DT 10_1101-2021_01_08_425864 79 40 license license NN 10_1101-2021_01_08_425864 79 41 to to TO 10_1101-2021_01_08_425864 79 42 display display VB 10_1101-2021_01_08_425864 79 43 the the DT 10_1101-2021_01_08_425864 79 44 preprint preprint NN 10_1101-2021_01_08_425864 79 45 in in IN 10_1101-2021_01_08_425864 79 46 perpetuity perpetuity NN 10_1101-2021_01_08_425864 79 47 . . . 10_1101-2021_01_08_425864 80 1 It -PRON- PRP 10_1101-2021_01_08_425864 80 2 is be VBZ 10_1101-2021_01_08_425864 80 3 The the DT 10_1101-2021_01_08_425864 80 4 copyright copyright NN 10_1101-2021_01_08_425864 80 5 holder holder NN 10_1101-2021_01_08_425864 80 6 for for IN 10_1101-2021_01_08_425864 80 7 this this DT 10_1101-2021_01_08_425864 80 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 80 9 version version NN 10_1101-2021_01_08_425864 80 10 posted post VBD 10_1101-2021_01_08_425864 80 11 January January NNP 10_1101-2021_01_08_425864 80 12 8 8 CD 10_1101-2021_01_08_425864 80 13 , , , 10_1101-2021_01_08_425864 80 14 2021 2021 CD 10_1101-2021_01_08_425864 80 15 . . . 10_1101-2021_01_08_425864 80 16 ; ; : 10_1101-2021_01_08_425864 80 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 80 18 : : : 10_1101-2021_01_08_425864 80 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 80 20 preprint preprint NN 10_1101-2021_01_08_425864 80 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 80 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 80 23 167 167 CD 10_1101-2021_01_08_425864 80 24 adding add VBG 10_1101-2021_01_08_425864 80 25 the the DT 10_1101-2021_01_08_425864 80 26 substrate substrate NN 10_1101-2021_01_08_425864 80 27 . . . 10_1101-2021_01_08_425864 81 1 All all PDT 10_1101-2021_01_08_425864 81 2 the the DT 10_1101-2021_01_08_425864 81 3 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 81 4 assays assay NNS 10_1101-2021_01_08_425864 81 5 were be VBD 10_1101-2021_01_08_425864 81 6 performed perform VBN 10_1101-2021_01_08_425864 81 7 in in IN 10_1101-2021_01_08_425864 81 8 96-well 96-well CD 10_1101-2021_01_08_425864 81 9 plates plate NNS 10_1101-2021_01_08_425864 81 10 at at IN 10_1101-2021_01_08_425864 81 11 a a DT 10_1101-2021_01_08_425864 81 12 final final JJ 10_1101-2021_01_08_425864 81 13 volume volume NN 10_1101-2021_01_08_425864 81 14 of of IN 10_1101-2021_01_08_425864 81 15 168 168 CD 10_1101-2021_01_08_425864 81 16 0.2 0.2 CD 10_1101-2021_01_08_425864 81 17 mL ml NN 10_1101-2021_01_08_425864 81 18 in in IN 10_1101-2021_01_08_425864 81 19 the the DT 10_1101-2021_01_08_425864 81 20 SpectraMax SpectraMax NNP 10_1101-2021_01_08_425864 81 21 ® ® NNPS 10_1101-2021_01_08_425864 81 22 i3 i3 NNP 10_1101-2021_01_08_425864 81 23 ( ( -LRB- 10_1101-2021_01_08_425864 81 24 Molecular Molecular NNP 10_1101-2021_01_08_425864 81 25 Devices Devices NNPS 10_1101-2021_01_08_425864 81 26 ) ) -RRB- 10_1101-2021_01_08_425864 81 27 . . . 10_1101-2021_01_08_425864 82 1 169 169 CD 10_1101-2021_01_08_425864 82 2 170 170 CD 10_1101-2021_01_08_425864 82 3 Acetyl Acetyl NNP 10_1101-2021_01_08_425864 82 4 - - HYPH 10_1101-2021_01_08_425864 82 5 CoA CoA NNP 10_1101-2021_01_08_425864 82 6 carboxylase carboxylase NN 10_1101-2021_01_08_425864 82 7 ( ( -LRB- 10_1101-2021_01_08_425864 82 8 ACC ACC NNP 10_1101-2021_01_08_425864 82 9 ) ) -RRB- 10_1101-2021_01_08_425864 82 10 . . . 10_1101-2021_01_08_425864 83 1 The the DT 10_1101-2021_01_08_425864 83 2 ACC ACC NNP 10_1101-2021_01_08_425864 83 3 activity activity NN 10_1101-2021_01_08_425864 83 4 was be VBD 10_1101-2021_01_08_425864 83 5 measured measure VBN 10_1101-2021_01_08_425864 83 6 spectrophotometrically spectrophotometrically RB 10_1101-2021_01_08_425864 83 7 by by IN 10_1101-2021_01_08_425864 83 8 171 171 CD 10_1101-2021_01_08_425864 83 9 coupling couple VBG 10_1101-2021_01_08_425864 83 10 its -PRON- PRP$ 10_1101-2021_01_08_425864 83 11 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 83 12 reaction reaction NN 10_1101-2021_01_08_425864 83 13 with with IN 10_1101-2021_01_08_425864 83 14 that that DT 10_1101-2021_01_08_425864 83 15 of of IN 10_1101-2021_01_08_425864 83 16 citrate citrate NN 10_1101-2021_01_08_425864 83 17 synthase synthase NN 10_1101-2021_01_08_425864 83 18 ( ( -LRB- 10_1101-2021_01_08_425864 83 19 CS CS NNP 10_1101-2021_01_08_425864 83 20 ) ) -RRB- 10_1101-2021_01_08_425864 83 21 , , , 10_1101-2021_01_08_425864 83 22 which which WDT 10_1101-2021_01_08_425864 83 23 uses use VBZ 10_1101-2021_01_08_425864 83 24 oxaloacetate oxaloacetate NN 10_1101-2021_01_08_425864 83 25 and and CC 10_1101-2021_01_08_425864 83 26 acetyl- acetyl- JJ 10_1101-2021_01_08_425864 83 27 172 172 CD 10_1101-2021_01_08_425864 83 28 CoA coa NN 10_1101-2021_01_08_425864 83 29 to to TO 10_1101-2021_01_08_425864 83 30 produce produce VB 10_1101-2021_01_08_425864 83 31 citrate citrate NN 10_1101-2021_01_08_425864 83 32 . . . 10_1101-2021_01_08_425864 84 1 Measurements measurement NNS 10_1101-2021_01_08_425864 84 2 were be VBD 10_1101-2021_01_08_425864 84 3 performed perform VBN 10_1101-2021_01_08_425864 84 4 at at IN 10_1101-2021_01_08_425864 84 5 the the DT 10_1101-2021_01_08_425864 84 6 end end NN 10_1101-2021_01_08_425864 84 7 - - HYPH 10_1101-2021_01_08_425864 84 8 points point NNS 10_1101-2021_01_08_425864 84 9 in in IN 10_1101-2021_01_08_425864 84 10 two two CD 10_1101-2021_01_08_425864 84 11 steps step NNS 10_1101-2021_01_08_425864 84 12 . . . 10_1101-2021_01_08_425864 85 1 First first RB 10_1101-2021_01_08_425864 85 2 , , , 10_1101-2021_01_08_425864 85 3 the the DT 10_1101-2021_01_08_425864 85 4 173 173 CD 10_1101-2021_01_08_425864 85 5 reaction reaction NN 10_1101-2021_01_08_425864 85 6 mixture mixture NN 10_1101-2021_01_08_425864 85 7 contained contain VBD 10_1101-2021_01_08_425864 85 8 100 100 CD 10_1101-2021_01_08_425864 85 9 mM mM NNP 10_1101-2021_01_08_425864 85 10 potassium potassium NN 10_1101-2021_01_08_425864 85 11 phosphate phosphate NN 10_1101-2021_01_08_425864 85 12 buffer buffer NN 10_1101-2021_01_08_425864 85 13 ( ( -LRB- 10_1101-2021_01_08_425864 85 14 pH pH NNP 10_1101-2021_01_08_425864 85 15 = = SYM 10_1101-2021_01_08_425864 85 16 8.0 8.0 CD 10_1101-2021_01_08_425864 85 17 ) ) -RRB- 10_1101-2021_01_08_425864 85 18 , , , 10_1101-2021_01_08_425864 85 19 15 15 CD 10_1101-2021_01_08_425864 85 20 mM mm NN 10_1101-2021_01_08_425864 85 21 KHCO3 KHCO3 NNP 10_1101-2021_01_08_425864 85 22 , , , 10_1101-2021_01_08_425864 85 23 5 5 CD 10_1101-2021_01_08_425864 85 24 mM mM NNP 10_1101-2021_01_08_425864 85 25 174 174 CD 10_1101-2021_01_08_425864 85 26 MnCl2 mncl2 CD 10_1101-2021_01_08_425864 85 27 , , , 10_1101-2021_01_08_425864 85 28 5 5 CD 10_1101-2021_01_08_425864 85 29 mM mm NN 10_1101-2021_01_08_425864 85 30 ATP atp NN 10_1101-2021_01_08_425864 85 31 , , , 10_1101-2021_01_08_425864 85 32 1 1 CD 10_1101-2021_01_08_425864 85 33 mM mm NN 10_1101-2021_01_08_425864 85 34 acetyl acetyl NN 10_1101-2021_01_08_425864 85 35 - - HYPH 10_1101-2021_01_08_425864 85 36 CoA CoA NNP 10_1101-2021_01_08_425864 85 37 and and CC 10_1101-2021_01_08_425864 85 38 0.1 0.1 CD 10_1101-2021_01_08_425864 85 39 μM μm NN 10_1101-2021_01_08_425864 85 40 biotin biotin JJ 10_1101-2021_01_08_425864 85 41 . . . 10_1101-2021_01_08_425864 86 1 The the DT 10_1101-2021_01_08_425864 86 2 reaction reaction NN 10_1101-2021_01_08_425864 86 3 was be VBD 10_1101-2021_01_08_425864 86 4 initiated initiate VBN 10_1101-2021_01_08_425864 86 5 by by IN 10_1101-2021_01_08_425864 86 6 adding add VBG 10_1101-2021_01_08_425864 86 7 0.1 0.1 CD 10_1101-2021_01_08_425864 86 8 175 175 CD 10_1101-2021_01_08_425864 86 9 mg mg NN 10_1101-2021_01_08_425864 86 10 of of IN 10_1101-2021_01_08_425864 86 11 cell cell NN 10_1101-2021_01_08_425864 86 12 extract extract NN 10_1101-2021_01_08_425864 86 13 and and CC 10_1101-2021_01_08_425864 86 14 developed develop VBD 10_1101-2021_01_08_425864 86 15 using use VBG 10_1101-2021_01_08_425864 86 16 15 15 CD 10_1101-2021_01_08_425864 86 17 min min NN 10_1101-2021_01_08_425864 86 18 incubations incubation NNS 10_1101-2021_01_08_425864 86 19 at at IN 10_1101-2021_01_08_425864 86 20 28 28 CD 10_1101-2021_01_08_425864 86 21 ° ° , 10_1101-2021_01_08_425864 86 22 C C NNP 10_1101-2021_01_08_425864 86 23 . . . 10_1101-2021_01_08_425864 87 1 The the DT 10_1101-2021_01_08_425864 87 2 reaction reaction NN 10_1101-2021_01_08_425864 87 3 was be VBD 10_1101-2021_01_08_425864 87 4 stopped stop VBN 10_1101-2021_01_08_425864 87 5 by by IN 10_1101-2021_01_08_425864 87 6 176 176 CD 10_1101-2021_01_08_425864 87 7 adding add VBG 10_1101-2021_01_08_425864 87 8 perchloric perchloric JJ 10_1101-2021_01_08_425864 87 9 acid acid NN 10_1101-2021_01_08_425864 87 10 40 40 CD 10_1101-2021_01_08_425864 87 11 % % NN 10_1101-2021_01_08_425864 87 12 ( ( -LRB- 10_1101-2021_01_08_425864 87 13 v v NN 10_1101-2021_01_08_425864 87 14 / / SYM 10_1101-2021_01_08_425864 87 15 v v NN 10_1101-2021_01_08_425864 87 16 ) ) -RRB- 10_1101-2021_01_08_425864 87 17 and and CC 10_1101-2021_01_08_425864 87 18 centrifuged centrifuge VBD 10_1101-2021_01_08_425864 87 19 10,000 10,000 CD 10_1101-2021_01_08_425864 87 20 x x SYM 10_1101-2021_01_08_425864 87 21 g g NN 10_1101-2021_01_08_425864 87 22 for for IN 10_1101-2021_01_08_425864 87 23 15 15 CD 10_1101-2021_01_08_425864 87 24 min min NN 10_1101-2021_01_08_425864 87 25 at at IN 10_1101-2021_01_08_425864 87 26 4 4 CD 10_1101-2021_01_08_425864 87 27 ° ° NNS 10_1101-2021_01_08_425864 87 28 C C NNP 10_1101-2021_01_08_425864 87 29 . . . 10_1101-2021_01_08_425864 88 1 The the DT 10_1101-2021_01_08_425864 88 2 second second JJ 10_1101-2021_01_08_425864 88 3 reaction reaction NN 10_1101-2021_01_08_425864 88 4 177 177 CD 10_1101-2021_01_08_425864 88 5 was be VBD 10_1101-2021_01_08_425864 88 6 performed perform VBN 10_1101-2021_01_08_425864 88 7 by by IN 10_1101-2021_01_08_425864 88 8 using use VBG 10_1101-2021_01_08_425864 88 9 0.1 0.1 CD 10_1101-2021_01_08_425864 88 10 mL ml NN 10_1101-2021_01_08_425864 88 11 of of IN 10_1101-2021_01_08_425864 88 12 the the DT 10_1101-2021_01_08_425864 88 13 supernatant supernatant JJ 10_1101-2021_01_08_425864 88 14 from from IN 10_1101-2021_01_08_425864 88 15 the the DT 10_1101-2021_01_08_425864 88 16 first first JJ 10_1101-2021_01_08_425864 88 17 reaction reaction NN 10_1101-2021_01_08_425864 88 18 , , , 10_1101-2021_01_08_425864 88 19 20 20 CD 10_1101-2021_01_08_425864 88 20 mM mm NN 10_1101-2021_01_08_425864 88 21 oxaloacetate oxaloacetate NN 10_1101-2021_01_08_425864 88 22 and and CC 10_1101-2021_01_08_425864 88 23 178 178 CD 10_1101-2021_01_08_425864 88 24 0.5 0.5 CD 10_1101-2021_01_08_425864 88 25 mM mm NN 10_1101-2021_01_08_425864 88 26 of of IN 10_1101-2021_01_08_425864 88 27 DTNB DTNB NNP 10_1101-2021_01_08_425864 88 28 in in IN 10_1101-2021_01_08_425864 88 29 100 100 CD 10_1101-2021_01_08_425864 88 30 mM mM NNP 10_1101-2021_01_08_425864 88 31 potassium potassium NN 10_1101-2021_01_08_425864 88 32 phosphate phosphate NN 10_1101-2021_01_08_425864 88 33 buffer buffer NN 10_1101-2021_01_08_425864 88 34 ( ( -LRB- 10_1101-2021_01_08_425864 88 35 pH pH NNP 10_1101-2021_01_08_425864 88 36 = = SYM 10_1101-2021_01_08_425864 88 37 8.0 8.0 CD 10_1101-2021_01_08_425864 88 38 ) ) -RRB- 10_1101-2021_01_08_425864 88 39 . . . 10_1101-2021_01_08_425864 89 1 The the DT 10_1101-2021_01_08_425864 89 2 reaction reaction NN 10_1101-2021_01_08_425864 89 3 was be VBD 10_1101-2021_01_08_425864 89 4 initiated initiate VBN 10_1101-2021_01_08_425864 89 5 by by IN 10_1101-2021_01_08_425864 89 6 179 179 CD 10_1101-2021_01_08_425864 89 7 adding add VBG 10_1101-2021_01_08_425864 89 8 0.5 0.5 CD 10_1101-2021_01_08_425864 89 9 units unit NNS 10_1101-2021_01_08_425864 89 10 of of IN 10_1101-2021_01_08_425864 89 11 CS CS NNP 10_1101-2021_01_08_425864 89 12 ( ( -LRB- 10_1101-2021_01_08_425864 89 13 Sigma Sigma NNP 10_1101-2021_01_08_425864 89 14 Aldrich Aldrich NNP 10_1101-2021_01_08_425864 89 15 © © NNP 10_1101-2021_01_08_425864 89 16 ) ) -RRB- 10_1101-2021_01_08_425864 89 17 . . . 10_1101-2021_01_08_425864 90 1 To to TO 10_1101-2021_01_08_425864 90 2 calculate calculate VB 10_1101-2021_01_08_425864 90 3 the the DT 10_1101-2021_01_08_425864 90 4 specific specific JJ 10_1101-2021_01_08_425864 90 5 activity activity NN 10_1101-2021_01_08_425864 90 6 of of IN 10_1101-2021_01_08_425864 90 7 ACC ACC NNP 10_1101-2021_01_08_425864 90 8 , , , 10_1101-2021_01_08_425864 90 9 we -PRON- PRP 10_1101-2021_01_08_425864 90 10 converted convert VBD 10_1101-2021_01_08_425864 90 11 180 180 CD 10_1101-2021_01_08_425864 90 12 the the DT 10_1101-2021_01_08_425864 90 13 absorbance absorbance NN 10_1101-2021_01_08_425864 90 14 values value NNS 10_1101-2021_01_08_425864 90 15 to to IN 10_1101-2021_01_08_425864 90 16 molarity molarity NN 10_1101-2021_01_08_425864 90 17 by by IN 10_1101-2021_01_08_425864 90 18 using use VBG 10_1101-2021_01_08_425864 90 19 the the DT 10_1101-2021_01_08_425864 90 20 TNB- tnb- JJ 10_1101-2021_01_08_425864 90 21 extinction extinction NN 10_1101-2021_01_08_425864 90 22 molar molar JJ 10_1101-2021_01_08_425864 90 23 coefficient coefficient NN 10_1101-2021_01_08_425864 90 24 of of IN 10_1101-2021_01_08_425864 90 25 12,000 12,000 CD 10_1101-2021_01_08_425864 90 26 M-1.s-1 M-1.s-1 NNS 10_1101-2021_01_08_425864 90 27 . . . 10_1101-2021_01_08_425864 91 1 181 181 CD 10_1101-2021_01_08_425864 91 2 For for IN 10_1101-2021_01_08_425864 91 3 the the DT 10_1101-2021_01_08_425864 91 4 blank blank JJ 10_1101-2021_01_08_425864 91 5 reaction reaction NN 10_1101-2021_01_08_425864 91 6 , , , 10_1101-2021_01_08_425864 91 7 we -PRON- PRP 10_1101-2021_01_08_425864 91 8 performed perform VBD 10_1101-2021_01_08_425864 91 9 the the DT 10_1101-2021_01_08_425864 91 10 same same JJ 10_1101-2021_01_08_425864 91 11 assay assay NN 10_1101-2021_01_08_425864 91 12 without without IN 10_1101-2021_01_08_425864 91 13 acetyl acetyl NN 10_1101-2021_01_08_425864 91 14 - - HYPH 10_1101-2021_01_08_425864 91 15 CoA CoA NNP 10_1101-2021_01_08_425864 91 16 [ [ -LRB- 10_1101-2021_01_08_425864 91 17 24 24 CD 10_1101-2021_01_08_425864 91 18 ] ] -RRB- 10_1101-2021_01_08_425864 91 19 . . . 10_1101-2021_01_08_425864 92 1 182 182 CD 10_1101-2021_01_08_425864 92 2 183 183 CD 10_1101-2021_01_08_425864 92 3 Hexokinase hexokinase NN 10_1101-2021_01_08_425864 92 4 ( ( -LRB- 10_1101-2021_01_08_425864 92 5 HK HK NNP 10_1101-2021_01_08_425864 92 6 ) ) -RRB- 10_1101-2021_01_08_425864 92 7 . . . 10_1101-2021_01_08_425864 93 1 The the DT 10_1101-2021_01_08_425864 93 2 HK HK NNP 10_1101-2021_01_08_425864 93 3 activity activity NN 10_1101-2021_01_08_425864 93 4 was be VBD 10_1101-2021_01_08_425864 93 5 measured measure VBN 10_1101-2021_01_08_425864 93 6 as as IN 10_1101-2021_01_08_425864 93 7 described describe VBN 10_1101-2021_01_08_425864 93 8 in in IN 10_1101-2021_01_08_425864 93 9 [ [ -LRB- 10_1101-2021_01_08_425864 93 10 25 25 CD 10_1101-2021_01_08_425864 93 11 ] ] -RRB- 10_1101-2021_01_08_425864 93 12 . . . 10_1101-2021_01_08_425864 94 1 Briefly briefly RB 10_1101-2021_01_08_425864 94 2 , , , 10_1101-2021_01_08_425864 94 3 the the DT 10_1101-2021_01_08_425864 94 4 activity activity NN 10_1101-2021_01_08_425864 94 5 was be VBD 10_1101-2021_01_08_425864 94 6 184 184 CD 10_1101-2021_01_08_425864 94 7 measured measure VBN 10_1101-2021_01_08_425864 94 8 by by IN 10_1101-2021_01_08_425864 94 9 coupling couple VBG 10_1101-2021_01_08_425864 94 10 the the DT 10_1101-2021_01_08_425864 94 11 hexokinase hexokinase NN 10_1101-2021_01_08_425864 94 12 activity activity NN 10_1101-2021_01_08_425864 94 13 with with IN 10_1101-2021_01_08_425864 94 14 a a DT 10_1101-2021_01_08_425864 94 15 commercial commercial JJ 10_1101-2021_01_08_425864 94 16 glucose-6-phosphate glucose-6-phosphate NNP 10_1101-2021_01_08_425864 94 17 185 185 CD 10_1101-2021_01_08_425864 94 18 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 94 19 , , , 10_1101-2021_01_08_425864 94 20 which which WDT 10_1101-2021_01_08_425864 94 21 oxidizes oxidize VBZ 10_1101-2021_01_08_425864 94 22 the the DT 10_1101-2021_01_08_425864 94 23 glucose-6-phosphate glucose-6-phosphate NNP 10_1101-2021_01_08_425864 94 24 ( ( -LRB- 10_1101-2021_01_08_425864 94 25 G6PD G6PD NNP 10_1101-2021_01_08_425864 94 26 , , , 10_1101-2021_01_08_425864 94 27 SIGMA SIGMA NNP 10_1101-2021_01_08_425864 94 28 ) ) -RRB- 10_1101-2021_01_08_425864 94 29 resulting result VBG 10_1101-2021_01_08_425864 94 30 from from IN 10_1101-2021_01_08_425864 94 31 the the DT 10_1101-2021_01_08_425864 94 32 HK HK NNP 10_1101-2021_01_08_425864 94 33 186 186 CD 10_1101-2021_01_08_425864 94 34 activity activity NN 10_1101-2021_01_08_425864 94 35 with with IN 10_1101-2021_01_08_425864 94 36 the the DT 10_1101-2021_01_08_425864 94 37 concomitant concomitant JJ 10_1101-2021_01_08_425864 94 38 reduction reduction NN 10_1101-2021_01_08_425864 94 39 of of IN 10_1101-2021_01_08_425864 94 40 NADP+ NADP+ NNP 10_1101-2021_01_08_425864 94 41 to to IN 10_1101-2021_01_08_425864 94 42 NADPH NADPH NNP 10_1101-2021_01_08_425864 94 43 . . . 10_1101-2021_01_08_425864 95 1 The the DT 10_1101-2021_01_08_425864 95 2 resulting result VBG 10_1101-2021_01_08_425864 95 3 NADPH NADPH NNP 10_1101-2021_01_08_425864 95 4 was be VBD 10_1101-2021_01_08_425864 95 5 187 187 CD 10_1101-2021_01_08_425864 95 6 spectrophotometrically spectrophotometrically RB 10_1101-2021_01_08_425864 95 7 monitored monitor VBN 10_1101-2021_01_08_425864 95 8 at at IN 10_1101-2021_01_08_425864 95 9 340 340 CD 10_1101-2021_01_08_425864 95 10 nm nm NN 10_1101-2021_01_08_425864 95 11 . . . 10_1101-2021_01_08_425864 96 1 The the DT 10_1101-2021_01_08_425864 96 2 reaction reaction NN 10_1101-2021_01_08_425864 96 3 mixture mixture NN 10_1101-2021_01_08_425864 96 4 contained contain VBD 10_1101-2021_01_08_425864 96 5 50 50 CD 10_1101-2021_01_08_425864 96 6 mM mM NNP 10_1101-2021_01_08_425864 96 7 Triethanolamine Triethanolamine NNP 10_1101-2021_01_08_425864 96 8 188 188 CD 10_1101-2021_01_08_425864 96 9 buffer buffer NN 10_1101-2021_01_08_425864 96 10 pH pH NNP 10_1101-2021_01_08_425864 96 11 7.5 7.5 CD 10_1101-2021_01_08_425864 96 12 , , , 10_1101-2021_01_08_425864 96 13 5 5 CD 10_1101-2021_01_08_425864 96 14 mM mM NNP 10_1101-2021_01_08_425864 96 15 MgCl2 mgcl2 NN 10_1101-2021_01_08_425864 96 16 , , , 10_1101-2021_01_08_425864 96 17 100 100 CD 10_1101-2021_01_08_425864 96 18 mM mM NNP 10_1101-2021_01_08_425864 96 19 KCl KCl NNS 10_1101-2021_01_08_425864 96 20 , , , 10_1101-2021_01_08_425864 96 21 10 10 CD 10_1101-2021_01_08_425864 96 22 mM mm NN 10_1101-2021_01_08_425864 96 23 glucose glucose NN 10_1101-2021_01_08_425864 96 24 , , , 10_1101-2021_01_08_425864 96 25 5 5 CD 10_1101-2021_01_08_425864 96 26 mM mm NN 10_1101-2021_01_08_425864 96 27 ATP atp NN 10_1101-2021_01_08_425864 96 28 and and CC 10_1101-2021_01_08_425864 96 29 5 5 CD 10_1101-2021_01_08_425864 96 30 U u NN 10_1101-2021_01_08_425864 96 31 of of IN 10_1101-2021_01_08_425864 96 32 commercial commercial JJ 10_1101-2021_01_08_425864 96 33 189 189 CD 10_1101-2021_01_08_425864 96 34 G6PD G6PD NNP 10_1101-2021_01_08_425864 96 35 . . . 10_1101-2021_01_08_425864 97 1 To to TO 10_1101-2021_01_08_425864 97 2 calculate calculate VB 10_1101-2021_01_08_425864 97 3 the the DT 10_1101-2021_01_08_425864 97 4 specific specific JJ 10_1101-2021_01_08_425864 97 5 activity activity NN 10_1101-2021_01_08_425864 97 6 , , , 10_1101-2021_01_08_425864 97 7 the the DT 10_1101-2021_01_08_425864 97 8 absorbance absorbance NN 10_1101-2021_01_08_425864 97 9 values value NNS 10_1101-2021_01_08_425864 97 10 were be VBD 10_1101-2021_01_08_425864 97 11 converted convert VBN 10_1101-2021_01_08_425864 97 12 to to IN 10_1101-2021_01_08_425864 97 13 molarity molarity NN 10_1101-2021_01_08_425864 97 14 using use VBG 10_1101-2021_01_08_425864 97 15 the the DT 10_1101-2021_01_08_425864 97 16 190 190 CD 10_1101-2021_01_08_425864 97 17 NADP(H NADP(H NNP 10_1101-2021_01_08_425864 97 18 ) ) -RRB- 10_1101-2021_01_08_425864 97 19 extinction extinction NN 10_1101-2021_01_08_425864 97 20 molar molar JJ 10_1101-2021_01_08_425864 97 21 coefficient coefficient NN 10_1101-2021_01_08_425864 97 22 of of IN 10_1101-2021_01_08_425864 97 23 6,220 6,220 CD 10_1101-2021_01_08_425864 97 24 M-1.s-1 M-1.s-1 NNS 10_1101-2021_01_08_425864 97 25 . . . 10_1101-2021_01_08_425864 98 1 191 191 CD 10_1101-2021_01_08_425864 98 2 Serine Serine NNP 10_1101-2021_01_08_425864 98 3 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 98 4 ( ( -LRB- 10_1101-2021_01_08_425864 98 5 SPT SPT NNP 10_1101-2021_01_08_425864 98 6 ) ) -RRB- 10_1101-2021_01_08_425864 98 7 . . . 10_1101-2021_01_08_425864 99 1 The the DT 10_1101-2021_01_08_425864 99 2 SPT SPT NNP 10_1101-2021_01_08_425864 99 3 activity activity NN 10_1101-2021_01_08_425864 99 4 was be VBD 10_1101-2021_01_08_425864 99 5 measured measure VBN 10_1101-2021_01_08_425864 99 6 through through IN 10_1101-2021_01_08_425864 99 7 the the DT 10_1101-2021_01_08_425864 99 8 reduction reduction NN 10_1101-2021_01_08_425864 99 9 of of IN 10_1101-2021_01_08_425864 99 10 the the DT 10_1101-2021_01_08_425864 99 11 192 192 CD 10_1101-2021_01_08_425864 99 12 DTNB dtnb NN 10_1101-2021_01_08_425864 99 13 reaction reaction NN 10_1101-2021_01_08_425864 99 14 by by IN 10_1101-2021_01_08_425864 99 15 the the DT 10_1101-2021_01_08_425864 99 16 free free JJ 10_1101-2021_01_08_425864 99 17 HS HS NNP 10_1101-2021_01_08_425864 99 18 - - HYPH 10_1101-2021_01_08_425864 99 19 CoA CoA NNP 10_1101-2021_01_08_425864 99 20 , , , 10_1101-2021_01_08_425864 99 21 forming form VBG 10_1101-2021_01_08_425864 99 22 the the DT 10_1101-2021_01_08_425864 99 23 TNB- tnb- JJ 10_1101-2021_01_08_425864 99 24 ion ion NN 10_1101-2021_01_08_425864 99 25 , , , 10_1101-2021_01_08_425864 99 26 which which WDT 10_1101-2021_01_08_425864 99 27 was be VBD 10_1101-2021_01_08_425864 99 28 measured measure VBN 10_1101-2021_01_08_425864 99 29 193 193 CD 10_1101-2021_01_08_425864 99 30 spectrophotometrically spectrophotometrically RB 10_1101-2021_01_08_425864 99 31 at at IN 10_1101-2021_01_08_425864 99 32 412 412 CD 10_1101-2021_01_08_425864 99 33 nm nm IN 10_1101-2021_01_08_425864 99 34 as as RB 10_1101-2021_01_08_425864 99 35 previously previously RB 10_1101-2021_01_08_425864 99 36 described describe VBN 10_1101-2021_01_08_425864 99 37 [ [ -LRB- 10_1101-2021_01_08_425864 99 38 23 23 CD 10_1101-2021_01_08_425864 99 39 ] ] -RRB- 10_1101-2021_01_08_425864 99 40 . . . 10_1101-2021_01_08_425864 100 1 In in IN 10_1101-2021_01_08_425864 100 2 brief brief NN 10_1101-2021_01_08_425864 100 3 , , , 10_1101-2021_01_08_425864 100 4 the the DT 10_1101-2021_01_08_425864 100 5 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 100 6 were be VBD 10_1101-2021_01_08_425864 100 7 194 194 CD 10_1101-2021_01_08_425864 100 8 washed wash VBN 10_1101-2021_01_08_425864 100 9 twice twice RB 10_1101-2021_01_08_425864 100 10 in in IN 10_1101-2021_01_08_425864 100 11 PBS PBS NNP 10_1101-2021_01_08_425864 100 12 , , , 10_1101-2021_01_08_425864 100 13 resuspended resuspend VBD 10_1101-2021_01_08_425864 100 14 in in IN 10_1101-2021_01_08_425864 100 15 Tris Tris NNP 10_1101-2021_01_08_425864 100 16 - - HYPH 10_1101-2021_01_08_425864 100 17 EDTA EDTA NNP 10_1101-2021_01_08_425864 100 18 buffer buffer NN 10_1101-2021_01_08_425864 100 19 ( ( -LRB- 10_1101-2021_01_08_425864 100 20 100 100 CD 10_1101-2021_01_08_425864 100 21 mM/2.5 mM/2.5 NNP 10_1101-2021_01_08_425864 100 22 mM mM NNP 10_1101-2021_01_08_425864 100 23 ) ) -RRB- 10_1101-2021_01_08_425864 100 24 containing contain VBG 10_1101-2021_01_08_425864 100 25 Triton Triton NNP 10_1101-2021_01_08_425864 100 26 X-100 X-100 NNP 10_1101-2021_01_08_425864 100 27 195 195 CD 10_1101-2021_01_08_425864 100 28 0.1 0.1 CD 10_1101-2021_01_08_425864 100 29 % % NN 10_1101-2021_01_08_425864 100 30 and and CC 10_1101-2021_01_08_425864 100 31 lysed lyse VBN 10_1101-2021_01_08_425864 100 32 by by IN 10_1101-2021_01_08_425864 100 33 sonication sonication NN 10_1101-2021_01_08_425864 100 34 ( ( -LRB- 10_1101-2021_01_08_425864 100 35 20 20 CD 10_1101-2021_01_08_425864 100 36 % % NN 10_1101-2021_01_08_425864 100 37 of of IN 10_1101-2021_01_08_425864 100 38 potency potency NN 10_1101-2021_01_08_425864 100 39 , , , 10_1101-2021_01_08_425864 100 40 during during IN 10_1101-2021_01_08_425864 100 41 2 2 CD 10_1101-2021_01_08_425864 100 42 min min NNP 10_1101-2021_01_08_425864 100 43 ) ) -RRB- 10_1101-2021_01_08_425864 100 44 . . . 10_1101-2021_01_08_425864 101 1 The the DT 10_1101-2021_01_08_425864 101 2 reaction reaction NN 10_1101-2021_01_08_425864 101 3 mixture mixture NN 10_1101-2021_01_08_425864 101 4 contained contain VBD 10_1101-2021_01_08_425864 101 5 0.1 0.1 CD 10_1101-2021_01_08_425864 101 6 196 196 CD 10_1101-2021_01_08_425864 101 7 mg mg CD 10_1101-2021_01_08_425864 101 8 of of IN 10_1101-2021_01_08_425864 101 9 protein protein NN 10_1101-2021_01_08_425864 101 10 free free JJ 10_1101-2021_01_08_425864 101 11 - - HYPH 10_1101-2021_01_08_425864 101 12 cell cell NN 10_1101-2021_01_08_425864 101 13 extract extract NN 10_1101-2021_01_08_425864 101 14 , , , 10_1101-2021_01_08_425864 101 15 0.5 0.5 CD 10_1101-2021_01_08_425864 101 16 mM mM NNP 10_1101-2021_01_08_425864 101 17 L L NNP 10_1101-2021_01_08_425864 101 18 - - HYPH 10_1101-2021_01_08_425864 101 19 serine serine NNP 10_1101-2021_01_08_425864 101 20 , , , 10_1101-2021_01_08_425864 101 21 0.1 0.1 CD 10_1101-2021_01_08_425864 101 22 mM mm NN 10_1101-2021_01_08_425864 101 23 palmitoyl palmitoyl NN 10_1101-2021_01_08_425864 101 24 - - HYPH 10_1101-2021_01_08_425864 101 25 CoA CoA NNP 10_1101-2021_01_08_425864 101 26 and and CC 10_1101-2021_01_08_425864 101 27 2.5 2.5 CD 10_1101-2021_01_08_425864 101 28 mM mM NNP 10_1101-2021_01_08_425864 101 29 DTNB dtnb NN 10_1101-2021_01_08_425864 101 30 in in IN 10_1101-2021_01_08_425864 101 31 Tris- Tris- NNP 10_1101-2021_01_08_425864 101 32 197 197 CD 10_1101-2021_01_08_425864 101 33 EDTA edta NN 10_1101-2021_01_08_425864 101 34 buffer buffer NN 10_1101-2021_01_08_425864 101 35 ( ( -LRB- 10_1101-2021_01_08_425864 101 36 100 100 CD 10_1101-2021_01_08_425864 101 37 mM/2.5 mM/2.5 NNP 10_1101-2021_01_08_425864 101 38 mM mM NNP 10_1101-2021_01_08_425864 101 39 ) ) -RRB- 10_1101-2021_01_08_425864 101 40 pH ph NN 10_1101-2021_01_08_425864 101 41 = = NFP 10_1101-2021_01_08_425864 101 42 8.0 8.0 CD 10_1101-2021_01_08_425864 101 43 [ [ -LRB- 10_1101-2021_01_08_425864 101 44 26 26 CD 10_1101-2021_01_08_425864 101 45 ] ] -RRB- 10_1101-2021_01_08_425864 101 46 . . . 10_1101-2021_01_08_425864 102 1 To to TO 10_1101-2021_01_08_425864 102 2 calculate calculate VB 10_1101-2021_01_08_425864 102 3 the the DT 10_1101-2021_01_08_425864 102 4 specific specific JJ 10_1101-2021_01_08_425864 102 5 activity activity NN 10_1101-2021_01_08_425864 102 6 , , , 10_1101-2021_01_08_425864 102 7 we -PRON- PRP 10_1101-2021_01_08_425864 102 8 converted convert VBD 10_1101-2021_01_08_425864 102 9 the the DT 10_1101-2021_01_08_425864 102 10 198 198 CD 10_1101-2021_01_08_425864 102 11 absorbance absorbance NN 10_1101-2021_01_08_425864 102 12 values value NNS 10_1101-2021_01_08_425864 102 13 to to IN 10_1101-2021_01_08_425864 102 14 molarity molarity NN 10_1101-2021_01_08_425864 102 15 using use VBG 10_1101-2021_01_08_425864 102 16 the the DT 10_1101-2021_01_08_425864 102 17 TNB- tnb- JJ 10_1101-2021_01_08_425864 102 18 extinction extinction NN 10_1101-2021_01_08_425864 102 19 molar molar JJ 10_1101-2021_01_08_425864 102 20 coefficient coefficient NN 10_1101-2021_01_08_425864 102 21 of of IN 10_1101-2021_01_08_425864 102 22 12,000 12,000 CD 10_1101-2021_01_08_425864 102 23 M-1.s-1 M-1.s-1 NNS 10_1101-2021_01_08_425864 102 24 . . . 10_1101-2021_01_08_425864 103 1 For for IN 10_1101-2021_01_08_425864 103 2 the the DT 10_1101-2021_01_08_425864 103 3 199 199 CD 10_1101-2021_01_08_425864 103 4 blank blank JJ 10_1101-2021_01_08_425864 103 5 reaction reaction NN 10_1101-2021_01_08_425864 103 6 , , , 10_1101-2021_01_08_425864 103 7 we -PRON- PRP 10_1101-2021_01_08_425864 103 8 performed perform VBD 10_1101-2021_01_08_425864 103 9 the the DT 10_1101-2021_01_08_425864 103 10 same same JJ 10_1101-2021_01_08_425864 103 11 assay assay NN 10_1101-2021_01_08_425864 103 12 without without IN 10_1101-2021_01_08_425864 103 13 adding add VBG 10_1101-2021_01_08_425864 103 14 palmitoyl palmitoyl NN 10_1101-2021_01_08_425864 103 15 - - : 10_1101-2021_01_08_425864 103 16 CoA. coa. NN 10_1101-2021_01_08_425864 104 1 All all PDT 10_1101-2021_01_08_425864 104 2 the the DT 10_1101-2021_01_08_425864 104 3 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 104 4 assays assays RB 10_1101-2021_01_08_425864 104 5 200 200 CD 10_1101-2021_01_08_425864 104 6 were be VBD 10_1101-2021_01_08_425864 104 7 performed perform VBN 10_1101-2021_01_08_425864 104 8 in in IN 10_1101-2021_01_08_425864 104 9 96-well 96-well CD 10_1101-2021_01_08_425864 104 10 plates plate NNS 10_1101-2021_01_08_425864 104 11 in in IN 10_1101-2021_01_08_425864 104 12 a a DT 10_1101-2021_01_08_425864 104 13 final final JJ 10_1101-2021_01_08_425864 104 14 volume volume NN 10_1101-2021_01_08_425864 104 15 of of IN 10_1101-2021_01_08_425864 104 16 0.2 0.2 CD 10_1101-2021_01_08_425864 104 17 mL ml NN 10_1101-2021_01_08_425864 104 18 in in IN 10_1101-2021_01_08_425864 104 19 the the DT 10_1101-2021_01_08_425864 104 20 SpectraMax SpectraMax NNP 10_1101-2021_01_08_425864 104 21 ® ® NNPS 10_1101-2021_01_08_425864 104 22 i3 i3 NNP 10_1101-2021_01_08_425864 104 23 ( ( -LRB- 10_1101-2021_01_08_425864 104 24 Molecular Molecular NNP 10_1101-2021_01_08_425864 104 25 201 201 CD 10_1101-2021_01_08_425864 104 26 Devices Devices NNPS 10_1101-2021_01_08_425864 104 27 ) ) -RRB- 10_1101-2021_01_08_425864 104 28 . . . 10_1101-2021_01_08_425864 105 1 .CC .CC NFP 10_1101-2021_01_08_425864 105 2 - - : 10_1101-2021_01_08_425864 105 3 BY by IN 10_1101-2021_01_08_425864 105 4 4.0 4.0 CD 10_1101-2021_01_08_425864 105 5 International international JJ 10_1101-2021_01_08_425864 105 6 licensemade licensemade NN 10_1101-2021_01_08_425864 105 7 available available JJ 10_1101-2021_01_08_425864 105 8 under under IN 10_1101-2021_01_08_425864 105 9 a a DT 10_1101-2021_01_08_425864 105 10 ( ( -LRB- 10_1101-2021_01_08_425864 105 11 which which WDT 10_1101-2021_01_08_425864 105 12 was be VBD 10_1101-2021_01_08_425864 105 13 not not RB 10_1101-2021_01_08_425864 105 14 certified certify VBN 10_1101-2021_01_08_425864 105 15 by by IN 10_1101-2021_01_08_425864 105 16 peer peer NN 10_1101-2021_01_08_425864 105 17 review review NN 10_1101-2021_01_08_425864 105 18 ) ) -RRB- 10_1101-2021_01_08_425864 105 19 is be VBZ 10_1101-2021_01_08_425864 105 20 the the DT 10_1101-2021_01_08_425864 105 21 author author NN 10_1101-2021_01_08_425864 105 22 / / SYM 10_1101-2021_01_08_425864 105 23 funder funder NN 10_1101-2021_01_08_425864 105 24 , , , 10_1101-2021_01_08_425864 105 25 who who WP 10_1101-2021_01_08_425864 105 26 has have VBZ 10_1101-2021_01_08_425864 105 27 granted grant VBN 10_1101-2021_01_08_425864 105 28 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 105 29 a a DT 10_1101-2021_01_08_425864 105 30 license license NN 10_1101-2021_01_08_425864 105 31 to to TO 10_1101-2021_01_08_425864 105 32 display display VB 10_1101-2021_01_08_425864 105 33 the the DT 10_1101-2021_01_08_425864 105 34 preprint preprint NN 10_1101-2021_01_08_425864 105 35 in in IN 10_1101-2021_01_08_425864 105 36 perpetuity perpetuity NN 10_1101-2021_01_08_425864 105 37 . . . 10_1101-2021_01_08_425864 106 1 It -PRON- PRP 10_1101-2021_01_08_425864 106 2 is be VBZ 10_1101-2021_01_08_425864 106 3 The the DT 10_1101-2021_01_08_425864 106 4 copyright copyright NN 10_1101-2021_01_08_425864 106 5 holder holder NN 10_1101-2021_01_08_425864 106 6 for for IN 10_1101-2021_01_08_425864 106 7 this this DT 10_1101-2021_01_08_425864 106 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 106 9 version version NN 10_1101-2021_01_08_425864 106 10 posted post VBD 10_1101-2021_01_08_425864 106 11 January January NNP 10_1101-2021_01_08_425864 106 12 8 8 CD 10_1101-2021_01_08_425864 106 13 , , , 10_1101-2021_01_08_425864 106 14 2021 2021 CD 10_1101-2021_01_08_425864 106 15 . . . 10_1101-2021_01_08_425864 106 16 ; ; : 10_1101-2021_01_08_425864 106 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 106 18 : : : 10_1101-2021_01_08_425864 106 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 106 20 preprint preprint NN 10_1101-2021_01_08_425864 106 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 106 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 106 23 202 202 CD 10_1101-2021_01_08_425864 106 24 203 203 CD 10_1101-2021_01_08_425864 106 25 Glucose Glucose NNP 10_1101-2021_01_08_425864 106 26 and and CC 10_1101-2021_01_08_425864 106 27 triglyceride triglyceride NN 10_1101-2021_01_08_425864 106 28 quantification quantification NN 10_1101-2021_01_08_425864 106 29 204 204 CD 10_1101-2021_01_08_425864 106 30 Spent spend VBN 10_1101-2021_01_08_425864 106 31 LIT LIT NNP 10_1101-2021_01_08_425864 106 32 medium medium NN 10_1101-2021_01_08_425864 106 33 from from IN 10_1101-2021_01_08_425864 106 34 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 106 35 cultures culture NNS 10_1101-2021_01_08_425864 106 36 was be VBD 10_1101-2021_01_08_425864 106 37 collected collect VBN 10_1101-2021_01_08_425864 106 38 by by IN 10_1101-2021_01_08_425864 106 39 recovering recover VBG 10_1101-2021_01_08_425864 106 40 the the DT 10_1101-2021_01_08_425864 106 41 supernatants supernatant NNS 10_1101-2021_01_08_425864 106 42 205 205 CD 10_1101-2021_01_08_425864 106 43 from from IN 10_1101-2021_01_08_425864 106 44 a a DT 10_1101-2021_01_08_425864 106 45 centrifugation centrifugation NN 10_1101-2021_01_08_425864 106 46 ( ( -LRB- 10_1101-2021_01_08_425864 106 47 10,000 10,000 CD 10_1101-2021_01_08_425864 106 48 x x SYM 10_1101-2021_01_08_425864 106 49 g g NN 10_1101-2021_01_08_425864 106 50 for for IN 10_1101-2021_01_08_425864 106 51 15 15 CD 10_1101-2021_01_08_425864 106 52 min min NN 10_1101-2021_01_08_425864 106 53 at at IN 10_1101-2021_01_08_425864 106 54 4 4 CD 10_1101-2021_01_08_425864 106 55 ° ° , 10_1101-2021_01_08_425864 106 56 C C NNP 10_1101-2021_01_08_425864 106 57 ) ) -RRB- 10_1101-2021_01_08_425864 106 58 . . . 10_1101-2021_01_08_425864 107 1 Each each DT 10_1101-2021_01_08_425864 107 2 sample sample NN 10_1101-2021_01_08_425864 107 3 of of IN 10_1101-2021_01_08_425864 107 4 spent spend VBN 10_1101-2021_01_08_425864 107 5 LIT LIT NNP 10_1101-2021_01_08_425864 107 6 was be VBD 10_1101-2021_01_08_425864 107 7 analysed analyse VBN 10_1101-2021_01_08_425864 107 8 for for IN 10_1101-2021_01_08_425864 107 9 its -PRON- PRP$ 10_1101-2021_01_08_425864 107 10 206 206 CD 10_1101-2021_01_08_425864 107 11 glucose glucose NN 10_1101-2021_01_08_425864 107 12 and and CC 10_1101-2021_01_08_425864 107 13 triglyceride triglyceride NN 10_1101-2021_01_08_425864 107 14 contents content NNS 10_1101-2021_01_08_425864 107 15 using use VBG 10_1101-2021_01_08_425864 107 16 commercial commercial JJ 10_1101-2021_01_08_425864 107 17 kits kit NNS 10_1101-2021_01_08_425864 107 18 ( ( -LRB- 10_1101-2021_01_08_425864 107 19 triglyceride triglyceride NNP 10_1101-2021_01_08_425864 107 20 monoreagent monoreagent NNP 10_1101-2021_01_08_425864 107 21 and and CC 10_1101-2021_01_08_425864 107 22 glucose glucose VB 10_1101-2021_01_08_425864 107 23 207 207 CD 10_1101-2021_01_08_425864 107 24 monoreagent monoreagent NN 10_1101-2021_01_08_425864 107 25 by by IN 10_1101-2021_01_08_425864 107 26 Bioclin Bioclin NNP 10_1101-2021_01_08_425864 107 27 Brazil Brazil NNP 10_1101-2021_01_08_425864 107 28 ) ) -RRB- 10_1101-2021_01_08_425864 107 29 according accord VBG 10_1101-2021_01_08_425864 107 30 to to IN 10_1101-2021_01_08_425864 107 31 the the DT 10_1101-2021_01_08_425864 107 32 manufacturer manufacturer NN 10_1101-2021_01_08_425864 107 33 ’s ’s POS 10_1101-2021_01_08_425864 107 34 instructions instruction NNS 10_1101-2021_01_08_425864 107 35 . . . 10_1101-2021_01_08_425864 108 1 These these DT 10_1101-2021_01_08_425864 108 2 kits kit NNS 10_1101-2021_01_08_425864 108 3 are be VBP 10_1101-2021_01_08_425864 108 4 based base VBN 10_1101-2021_01_08_425864 108 5 on on IN 10_1101-2021_01_08_425864 108 6 208 208 CD 10_1101-2021_01_08_425864 108 7 colorimetric colorimetric JJ 10_1101-2021_01_08_425864 108 8 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 108 9 reactions reaction NNS 10_1101-2021_01_08_425864 108 10 , , , 10_1101-2021_01_08_425864 108 11 and and CC 10_1101-2021_01_08_425864 108 12 the the DT 10_1101-2021_01_08_425864 108 13 absorbance absorbance NN 10_1101-2021_01_08_425864 108 14 of of IN 10_1101-2021_01_08_425864 108 15 each each DT 10_1101-2021_01_08_425864 108 16 assay assay NN 10_1101-2021_01_08_425864 108 17 was be VBD 10_1101-2021_01_08_425864 108 18 measured measure VBN 10_1101-2021_01_08_425864 108 19 in in IN 10_1101-2021_01_08_425864 108 20 96-well 96-well CD 10_1101-2021_01_08_425864 108 21 plates plate NNS 10_1101-2021_01_08_425864 108 22 209 209 CD 10_1101-2021_01_08_425864 108 23 at at IN 10_1101-2021_01_08_425864 108 24 a a DT 10_1101-2021_01_08_425864 108 25 final final JJ 10_1101-2021_01_08_425864 108 26 volume volume NN 10_1101-2021_01_08_425864 108 27 of of IN 10_1101-2021_01_08_425864 108 28 0.2 0.2 CD 10_1101-2021_01_08_425864 108 29 mL ml NN 10_1101-2021_01_08_425864 108 30 in in IN 10_1101-2021_01_08_425864 108 31 the the DT 10_1101-2021_01_08_425864 108 32 SpectraMax SpectraMax NNP 10_1101-2021_01_08_425864 108 33 ® ® NNPS 10_1101-2021_01_08_425864 108 34 i3 i3 NNP 10_1101-2021_01_08_425864 108 35 ( ( -LRB- 10_1101-2021_01_08_425864 108 36 Molecular Molecular NNP 10_1101-2021_01_08_425864 108 37 Devices Devices NNPS 10_1101-2021_01_08_425864 108 38 ) ) -RRB- 10_1101-2021_01_08_425864 108 39 . . . 10_1101-2021_01_08_425864 109 1 210 210 CD 10_1101-2021_01_08_425864 109 2 211 211 CD 10_1101-2021_01_08_425864 109 3 Proliferation Proliferation NNP 10_1101-2021_01_08_425864 109 4 assays assays RB 10_1101-2021_01_08_425864 109 5 212 212 CD 10_1101-2021_01_08_425864 109 6 Exponentially exponentially RB 10_1101-2021_01_08_425864 109 7 growing grow VBG 10_1101-2021_01_08_425864 109 8 T. t. NN 10_1101-2021_01_08_425864 109 9 cruzi cruzi NNS 10_1101-2021_01_08_425864 109 10 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 109 11 ( ( -LRB- 10_1101-2021_01_08_425864 109 12 5x107 5x107 CD 10_1101-2021_01_08_425864 109 13 mL-1 mL-1 NNP 10_1101-2021_01_08_425864 109 14 ) ) -RRB- 10_1101-2021_01_08_425864 109 15 were be VBD 10_1101-2021_01_08_425864 109 16 treated treat VBN 10_1101-2021_01_08_425864 109 17 with with IN 10_1101-2021_01_08_425864 109 18 different different JJ 10_1101-2021_01_08_425864 109 19 213 213 CD 10_1101-2021_01_08_425864 109 20 concentrations concentration NNS 10_1101-2021_01_08_425864 109 21 of of IN 10_1101-2021_01_08_425864 109 22 ETO ETO NNP 10_1101-2021_01_08_425864 109 23 or or CC 10_1101-2021_01_08_425864 109 24 not not RB 10_1101-2021_01_08_425864 109 25 treated treat VBN 10_1101-2021_01_08_425864 109 26 ( ( -LRB- 10_1101-2021_01_08_425864 109 27 negative negative JJ 10_1101-2021_01_08_425864 109 28 control control NN 10_1101-2021_01_08_425864 109 29 ) ) -RRB- 10_1101-2021_01_08_425864 109 30 in in IN 10_1101-2021_01_08_425864 109 31 LIT LIT NNP 10_1101-2021_01_08_425864 109 32 medium medium NN 10_1101-2021_01_08_425864 109 33 . . . 10_1101-2021_01_08_425864 110 1 As as IN 10_1101-2021_01_08_425864 110 2 a a DT 10_1101-2021_01_08_425864 110 3 positive positive JJ 10_1101-2021_01_08_425864 110 4 control control NN 10_1101-2021_01_08_425864 110 5 for for IN 10_1101-2021_01_08_425864 110 6 214 214 CD 10_1101-2021_01_08_425864 110 7 growth growth NN 10_1101-2021_01_08_425864 110 8 inhibition inhibition NN 10_1101-2021_01_08_425864 110 9 , , , 10_1101-2021_01_08_425864 110 10 we -PRON- PRP 10_1101-2021_01_08_425864 110 11 used use VBD 10_1101-2021_01_08_425864 110 12 a a DT 10_1101-2021_01_08_425864 110 13 combination combination NN 10_1101-2021_01_08_425864 110 14 of of IN 10_1101-2021_01_08_425864 110 15 rotenone rotenone NN 10_1101-2021_01_08_425864 110 16 ( ( -LRB- 10_1101-2021_01_08_425864 110 17 60 60 CD 10_1101-2021_01_08_425864 110 18 µM µM NNP 10_1101-2021_01_08_425864 110 19 ) ) -RRB- 10_1101-2021_01_08_425864 110 20 and and CC 10_1101-2021_01_08_425864 110 21 antimycin antimycin NNS 10_1101-2021_01_08_425864 110 22 ( ( -LRB- 10_1101-2021_01_08_425864 110 23 0.5 0.5 CD 10_1101-2021_01_08_425864 110 24 µM µm NN 10_1101-2021_01_08_425864 110 25 ) ) -RRB- 10_1101-2021_01_08_425864 110 26 [ [ -LRB- 10_1101-2021_01_08_425864 110 27 27 27 CD 10_1101-2021_01_08_425864 110 28 ] ] -RRB- 10_1101-2021_01_08_425864 110 29 . . . 10_1101-2021_01_08_425864 111 1 The the DT 10_1101-2021_01_08_425864 111 2 215 215 CD 10_1101-2021_01_08_425864 111 3 parasites parasite NNS 10_1101-2021_01_08_425864 111 4 ( ( -LRB- 10_1101-2021_01_08_425864 111 5 2.5x106 2.5x106 NNP 10_1101-2021_01_08_425864 111 6 mL-1 mL-1 NNP 10_1101-2021_01_08_425864 111 7 ) ) -RRB- 10_1101-2021_01_08_425864 111 8 were be VBD 10_1101-2021_01_08_425864 111 9 transferred transfer VBN 10_1101-2021_01_08_425864 111 10 to to IN 10_1101-2021_01_08_425864 111 11 96-well 96-well CD 10_1101-2021_01_08_425864 111 12 plates plate NNS 10_1101-2021_01_08_425864 111 13 and and CC 10_1101-2021_01_08_425864 111 14 then then RB 10_1101-2021_01_08_425864 111 15 incubated incubate VBD 10_1101-2021_01_08_425864 111 16 at at IN 10_1101-2021_01_08_425864 111 17 28 28 CD 10_1101-2021_01_08_425864 111 18 ° ° , 10_1101-2021_01_08_425864 111 19 C C NNP 10_1101-2021_01_08_425864 111 20 . . . 10_1101-2021_01_08_425864 112 1 The the DT 10_1101-2021_01_08_425864 112 2 cell cell NN 10_1101-2021_01_08_425864 112 3 216 216 CD 10_1101-2021_01_08_425864 112 4 proliferation proliferation NN 10_1101-2021_01_08_425864 112 5 was be VBD 10_1101-2021_01_08_425864 112 6 quantified quantify VBN 10_1101-2021_01_08_425864 112 7 by by IN 10_1101-2021_01_08_425864 112 8 reading read VBG 10_1101-2021_01_08_425864 112 9 the the DT 10_1101-2021_01_08_425864 112 10 optical optical JJ 10_1101-2021_01_08_425864 112 11 density density NN 10_1101-2021_01_08_425864 112 12 ( ( -LRB- 10_1101-2021_01_08_425864 112 13 OD OD NNP 10_1101-2021_01_08_425864 112 14 ) ) -RRB- 10_1101-2021_01_08_425864 112 15 at at IN 10_1101-2021_01_08_425864 112 16 620 620 CD 10_1101-2021_01_08_425864 112 17 nm nm CD 10_1101-2021_01_08_425864 112 18 for for IN 10_1101-2021_01_08_425864 112 19 eight eight CD 10_1101-2021_01_08_425864 112 20 days day NNS 10_1101-2021_01_08_425864 112 21 . . . 10_1101-2021_01_08_425864 113 1 The the DT 10_1101-2021_01_08_425864 113 2 OD OD NNP 10_1101-2021_01_08_425864 113 3 217 217 CD 10_1101-2021_01_08_425864 113 4 values value NNS 10_1101-2021_01_08_425864 113 5 were be VBD 10_1101-2021_01_08_425864 113 6 converted convert VBN 10_1101-2021_01_08_425864 113 7 to to IN 10_1101-2021_01_08_425864 113 8 cell cell NN 10_1101-2021_01_08_425864 113 9 numbers number NNS 10_1101-2021_01_08_425864 113 10 using use VBG 10_1101-2021_01_08_425864 113 11 a a DT 10_1101-2021_01_08_425864 113 12 linear linear JJ 10_1101-2021_01_08_425864 113 13 regression regression NN 10_1101-2021_01_08_425864 113 14 equation equation NN 10_1101-2021_01_08_425864 113 15 previously previously RB 10_1101-2021_01_08_425864 113 16 obtained obtain VBN 10_1101-2021_01_08_425864 113 17 under under IN 10_1101-2021_01_08_425864 113 18 218 218 CD 10_1101-2021_01_08_425864 113 19 the the DT 10_1101-2021_01_08_425864 113 20 same same JJ 10_1101-2021_01_08_425864 113 21 conditions condition NNS 10_1101-2021_01_08_425864 113 22 . . . 10_1101-2021_01_08_425864 114 1 Each each DT 10_1101-2021_01_08_425864 114 2 experiment experiment NN 10_1101-2021_01_08_425864 114 3 was be VBD 10_1101-2021_01_08_425864 114 4 performed perform VBN 10_1101-2021_01_08_425864 114 5 in in IN 10_1101-2021_01_08_425864 114 6 quadruplicate quadruplicate NN 10_1101-2021_01_08_425864 114 7 [ [ -LRB- 10_1101-2021_01_08_425864 114 8 28 28 CD 10_1101-2021_01_08_425864 114 9 ] ] -RRB- 10_1101-2021_01_08_425864 114 10 . . . 10_1101-2021_01_08_425864 115 1 219 219 CD 10_1101-2021_01_08_425864 115 2 220 220 CD 10_1101-2021_01_08_425864 115 3 Flow flow NN 10_1101-2021_01_08_425864 115 4 cytometry cytometry NN 10_1101-2021_01_08_425864 115 5 analyses analyse VBZ 10_1101-2021_01_08_425864 115 6 221 221 CD 10_1101-2021_01_08_425864 115 7 Cell Cell NNP 10_1101-2021_01_08_425864 115 8 death death NN 10_1101-2021_01_08_425864 115 9 . . . 10_1101-2021_01_08_425864 116 1 Epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 116 2 in in IN 10_1101-2021_01_08_425864 116 3 the the DT 10_1101-2021_01_08_425864 116 4 exponential exponential JJ 10_1101-2021_01_08_425864 116 5 phase phase NN 10_1101-2021_01_08_425864 116 6 of of IN 10_1101-2021_01_08_425864 116 7 growth growth NN 10_1101-2021_01_08_425864 116 8 were be VBD 10_1101-2021_01_08_425864 116 9 maintained maintain VBN 10_1101-2021_01_08_425864 116 10 in in IN 10_1101-2021_01_08_425864 116 11 LIT LIT NNP 10_1101-2021_01_08_425864 116 12 and and CC 10_1101-2021_01_08_425864 116 13 treated treat VBD 10_1101-2021_01_08_425864 116 14 222 222 CD 10_1101-2021_01_08_425864 116 15 with with IN 10_1101-2021_01_08_425864 116 16 ETO ETO NNP 10_1101-2021_01_08_425864 116 17 500 500 CD 10_1101-2021_01_08_425864 116 18 µM µM NNP 10_1101-2021_01_08_425864 116 19 for for IN 10_1101-2021_01_08_425864 116 20 5 5 CD 10_1101-2021_01_08_425864 116 21 days day NNS 10_1101-2021_01_08_425864 116 22 . . . 10_1101-2021_01_08_425864 117 1 After after IN 10_1101-2021_01_08_425864 117 2 the the DT 10_1101-2021_01_08_425864 117 3 incubation incubation NN 10_1101-2021_01_08_425864 117 4 time time NN 10_1101-2021_01_08_425864 117 5 , , , 10_1101-2021_01_08_425864 117 6 the the DT 10_1101-2021_01_08_425864 117 7 parasites parasite NNS 10_1101-2021_01_08_425864 117 8 were be VBD 10_1101-2021_01_08_425864 117 9 analysed analyse VBN 10_1101-2021_01_08_425864 117 10 as as IN 10_1101-2021_01_08_425864 117 11 described describe VBN 10_1101-2021_01_08_425864 117 12 in in IN 10_1101-2021_01_08_425864 117 13 223 223 CD 10_1101-2021_01_08_425864 117 14 [ [ -LRB- 10_1101-2021_01_08_425864 117 15 28 28 CD 10_1101-2021_01_08_425864 117 16 ] ] -RRB- 10_1101-2021_01_08_425864 117 17 . . . 10_1101-2021_01_08_425864 118 1 The the DT 10_1101-2021_01_08_425864 118 2 cells cell NNS 10_1101-2021_01_08_425864 118 3 were be VBD 10_1101-2021_01_08_425864 118 4 analysed analyse VBN 10_1101-2021_01_08_425864 118 5 by by IN 10_1101-2021_01_08_425864 118 6 flow flow NN 10_1101-2021_01_08_425864 118 7 cytometry cytometry NN 10_1101-2021_01_08_425864 118 8 ( ( -LRB- 10_1101-2021_01_08_425864 118 9 FACScalibur FACScalibur NNP 10_1101-2021_01_08_425864 118 10 BD BD NNP 10_1101-2021_01_08_425864 118 11 Biosciences Biosciences NNP 10_1101-2021_01_08_425864 118 12 ) ) -RRB- 10_1101-2021_01_08_425864 118 13 . . . 10_1101-2021_01_08_425864 119 1 224 224 CD 10_1101-2021_01_08_425864 119 2 225 225 CD 10_1101-2021_01_08_425864 119 3 Cell cell NN 10_1101-2021_01_08_425864 119 4 cycle cycle NN 10_1101-2021_01_08_425864 119 5 ( ( -LRB- 10_1101-2021_01_08_425864 119 6 DNA dna NN 10_1101-2021_01_08_425864 119 7 content content NN 10_1101-2021_01_08_425864 119 8 ) ) -RRB- 10_1101-2021_01_08_425864 119 9 . . . 10_1101-2021_01_08_425864 120 1 Epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 120 2 in in IN 10_1101-2021_01_08_425864 120 3 the the DT 10_1101-2021_01_08_425864 120 4 exponential exponential JJ 10_1101-2021_01_08_425864 120 5 phase phase NN 10_1101-2021_01_08_425864 120 6 of of IN 10_1101-2021_01_08_425864 120 7 growth growth NN 10_1101-2021_01_08_425864 120 8 were be VBD 10_1101-2021_01_08_425864 120 9 maintained maintain VBN 10_1101-2021_01_08_425864 120 10 in in IN 10_1101-2021_01_08_425864 120 11 LIT LIT NNP 10_1101-2021_01_08_425864 120 12 226 226 CD 10_1101-2021_01_08_425864 120 13 and and CC 10_1101-2021_01_08_425864 120 14 treated treat VBN 10_1101-2021_01_08_425864 120 15 with with IN 10_1101-2021_01_08_425864 120 16 ETO ETO NNP 10_1101-2021_01_08_425864 120 17 500 500 CD 10_1101-2021_01_08_425864 120 18 µM µM NNP 10_1101-2021_01_08_425864 120 19 over over IN 10_1101-2021_01_08_425864 120 20 5 5 CD 10_1101-2021_01_08_425864 120 21 days day NNS 10_1101-2021_01_08_425864 120 22 . . . 10_1101-2021_01_08_425864 121 1 After after IN 10_1101-2021_01_08_425864 121 2 the the DT 10_1101-2021_01_08_425864 121 3 incubation incubation NN 10_1101-2021_01_08_425864 121 4 time time NN 10_1101-2021_01_08_425864 121 5 , , , 10_1101-2021_01_08_425864 121 6 the the DT 10_1101-2021_01_08_425864 121 7 parasites parasite NNS 10_1101-2021_01_08_425864 121 8 were be VBD 10_1101-2021_01_08_425864 121 9 washed wash VBN 10_1101-2021_01_08_425864 121 10 twice twice RB 10_1101-2021_01_08_425864 121 11 227 227 CD 10_1101-2021_01_08_425864 121 12 in in IN 10_1101-2021_01_08_425864 121 13 PBS PBS NNP 10_1101-2021_01_08_425864 121 14 and and CC 10_1101-2021_01_08_425864 121 15 resuspended resuspend VBD 10_1101-2021_01_08_425864 121 16 in in IN 10_1101-2021_01_08_425864 121 17 lysis lysis NN 10_1101-2021_01_08_425864 121 18 buffer buffer NNP 10_1101-2021_01_08_425864 121 19 ( ( -LRB- 10_1101-2021_01_08_425864 121 20 phosphate phosphate NN 10_1101-2021_01_08_425864 121 21 buffer buffer NNP 10_1101-2021_01_08_425864 121 22 Na2HPO4 Na2HPO4 NNP 10_1101-2021_01_08_425864 121 23 7.7 7.7 CD 10_1101-2021_01_08_425864 121 24 mM mM NNP 10_1101-2021_01_08_425864 121 25 ; ; : 10_1101-2021_01_08_425864 121 26 KH2PO4 KH2PO4 NNP 10_1101-2021_01_08_425864 121 27 2.3 2.3 CD 10_1101-2021_01_08_425864 121 28 mM mM NNP 10_1101-2021_01_08_425864 121 29 ; ; : 10_1101-2021_01_08_425864 121 30 pH pH NNP 10_1101-2021_01_08_425864 121 31 = = NFP 10_1101-2021_01_08_425864 121 32 228 228 CD 10_1101-2021_01_08_425864 121 33 7.4 7.4 CD 10_1101-2021_01_08_425864 121 34 ) ) -RRB- 10_1101-2021_01_08_425864 121 35 and and CC 10_1101-2021_01_08_425864 121 36 digitonin digitonin NN 10_1101-2021_01_08_425864 121 37 64 64 CD 10_1101-2021_01_08_425864 121 38 µM. µM. NNS 10_1101-2021_01_08_425864 122 1 After after IN 10_1101-2021_01_08_425864 122 2 incubating incubate VBG 10_1101-2021_01_08_425864 122 3 on on IN 10_1101-2021_01_08_425864 122 4 ice ice NN 10_1101-2021_01_08_425864 122 5 for for IN 10_1101-2021_01_08_425864 122 6 30 30 CD 10_1101-2021_01_08_425864 122 7 min min NN 10_1101-2021_01_08_425864 122 8 , , , 10_1101-2021_01_08_425864 122 9 propidium propidium NN 10_1101-2021_01_08_425864 122 10 iodide iodide NNP 10_1101-2021_01_08_425864 122 11 0.2 0.2 CD 10_1101-2021_01_08_425864 122 12 μg μg NN 10_1101-2021_01_08_425864 122 13 / / SYM 10_1101-2021_01_08_425864 122 14 mL mL NNP 10_1101-2021_01_08_425864 122 15 was be VBD 10_1101-2021_01_08_425864 122 16 added add VBN 10_1101-2021_01_08_425864 122 17 . . . 10_1101-2021_01_08_425864 123 1 229 229 CD 10_1101-2021_01_08_425864 123 2 The the DT 10_1101-2021_01_08_425864 123 3 samples sample NNS 10_1101-2021_01_08_425864 123 4 were be VBD 10_1101-2021_01_08_425864 123 5 analysed analyse VBN 10_1101-2021_01_08_425864 123 6 by by IN 10_1101-2021_01_08_425864 123 7 flow flow NN 10_1101-2021_01_08_425864 123 8 cytometry cytometry NN 10_1101-2021_01_08_425864 123 9 ( ( -LRB- 10_1101-2021_01_08_425864 123 10 Guava Guava NNP 10_1101-2021_01_08_425864 123 11 ) ) -RRB- 10_1101-2021_01_08_425864 123 12 adapted adapt VBD 10_1101-2021_01_08_425864 123 13 from from IN 10_1101-2021_01_08_425864 123 14 [ [ -LRB- 10_1101-2021_01_08_425864 123 15 29 29 CD 10_1101-2021_01_08_425864 123 16 ] ] -RRB- 10_1101-2021_01_08_425864 123 17 . . . 10_1101-2021_01_08_425864 124 1 230 230 CD 10_1101-2021_01_08_425864 124 2 231 231 CD 10_1101-2021_01_08_425864 124 3 Fatty Fatty NNP 10_1101-2021_01_08_425864 124 4 acid acid NN 10_1101-2021_01_08_425864 124 5 staining stain VBG 10_1101-2021_01_08_425864 124 6 using use VBG 10_1101-2021_01_08_425864 124 7 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 124 8 ® ® NNPS 10_1101-2021_01_08_425864 124 9 500/510 500/510 NNP 10_1101-2021_01_08_425864 124 10 . . . 10_1101-2021_01_08_425864 125 1 Exponentially exponentially RB 10_1101-2021_01_08_425864 125 2 growing grow VBG 10_1101-2021_01_08_425864 125 3 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 125 4 were be VBD 10_1101-2021_01_08_425864 125 5 kept keep VBN 10_1101-2021_01_08_425864 125 6 in in IN 10_1101-2021_01_08_425864 125 7 232 232 CD 10_1101-2021_01_08_425864 125 8 LIT LIT NNP 10_1101-2021_01_08_425864 125 9 medium medium NN 10_1101-2021_01_08_425864 125 10 to to TO 10_1101-2021_01_08_425864 125 11 reach reach VB 10_1101-2021_01_08_425864 125 12 three three CD 10_1101-2021_01_08_425864 125 13 different different JJ 10_1101-2021_01_08_425864 125 14 cell cell NN 10_1101-2021_01_08_425864 125 15 densities density NNS 10_1101-2021_01_08_425864 125 16 ( ( -LRB- 10_1101-2021_01_08_425864 125 17 2.5x107 2.5x107 NNP 10_1101-2021_01_08_425864 125 18 mL-1 mL-1 NNP 10_1101-2021_01_08_425864 125 19 , , , 10_1101-2021_01_08_425864 125 20 5x107 5x107 CD 10_1101-2021_01_08_425864 125 21 mL-1 ml-1 NN 10_1101-2021_01_08_425864 125 22 and and CC 10_1101-2021_01_08_425864 125 23 108 108 CD 10_1101-2021_01_08_425864 125 24 mL-1 ml-1 NN 10_1101-2021_01_08_425864 125 25 ) ) -RRB- 10_1101-2021_01_08_425864 125 26 in in IN 10_1101-2021_01_08_425864 125 27 24- 24- NNP 10_1101-2021_01_08_425864 125 28 233 233 CD 10_1101-2021_01_08_425864 125 29 well well NN 10_1101-2021_01_08_425864 125 30 plates plate NNS 10_1101-2021_01_08_425864 125 31 at at IN 10_1101-2021_01_08_425864 125 32 28 28 CD 10_1101-2021_01_08_425864 125 33 ° ° , 10_1101-2021_01_08_425864 125 34 C C NNP 10_1101-2021_01_08_425864 125 35 . . . 10_1101-2021_01_08_425864 126 1 Twenty twenty CD 10_1101-2021_01_08_425864 126 2 - - HYPH 10_1101-2021_01_08_425864 126 3 four four CD 10_1101-2021_01_08_425864 126 4 hours hour NNS 10_1101-2021_01_08_425864 126 5 before before IN 10_1101-2021_01_08_425864 126 6 the the DT 10_1101-2021_01_08_425864 126 7 flow flow NN 10_1101-2021_01_08_425864 126 8 cytometry cytometry NN 10_1101-2021_01_08_425864 126 9 analysis analysis NN 10_1101-2021_01_08_425864 126 10 , , , 10_1101-2021_01_08_425864 126 11 the the DT 10_1101-2021_01_08_425864 126 12 parasites parasite NNS 10_1101-2021_01_08_425864 126 13 were be VBD 10_1101-2021_01_08_425864 126 14 treated treat VBN 10_1101-2021_01_08_425864 126 15 234 234 CD 10_1101-2021_01_08_425864 126 16 with with IN 10_1101-2021_01_08_425864 126 17 1 1 CD 10_1101-2021_01_08_425864 126 18 µM µM NNP 10_1101-2021_01_08_425864 126 19 C1-BODIPY C1-BODIPY NNP 10_1101-2021_01_08_425864 126 20 ® ® NNPS 10_1101-2021_01_08_425864 126 21 500/510-C12 500/510-c12 CD 10_1101-2021_01_08_425864 126 22 . . . 10_1101-2021_01_08_425864 127 1 This this DT 10_1101-2021_01_08_425864 127 2 fluorophore fluorophore NN 10_1101-2021_01_08_425864 127 3 allows allow VBZ 10_1101-2021_01_08_425864 127 4 for for IN 10_1101-2021_01_08_425864 127 5 measurements measurement NNS 10_1101-2021_01_08_425864 127 6 of of IN 10_1101-2021_01_08_425864 127 7 the the DT 10_1101-2021_01_08_425864 127 8 relationship relationship NN 10_1101-2021_01_08_425864 127 9 235 235 CD 10_1101-2021_01_08_425864 127 10 between between IN 10_1101-2021_01_08_425864 127 11 fatty fatty NN 10_1101-2021_01_08_425864 127 12 acid acid NN 10_1101-2021_01_08_425864 127 13 accumulation accumulation NN 10_1101-2021_01_08_425864 127 14 and and CC 10_1101-2021_01_08_425864 127 15 consumption consumption NN 10_1101-2021_01_08_425864 127 16 by by IN 10_1101-2021_01_08_425864 127 17 shifting shift VBG 10_1101-2021_01_08_425864 127 18 the the DT 10_1101-2021_01_08_425864 127 19 fluorescence fluorescence NN 10_1101-2021_01_08_425864 127 20 filter filter NN 10_1101-2021_01_08_425864 127 21 . . . 10_1101-2021_01_08_425864 128 1 The the DT 10_1101-2021_01_08_425864 128 2 samples sample NNS 10_1101-2021_01_08_425864 128 3 236 236 CD 10_1101-2021_01_08_425864 128 4 were be VBD 10_1101-2021_01_08_425864 128 5 collected collect VBN 10_1101-2021_01_08_425864 128 6 , , , 10_1101-2021_01_08_425864 128 7 washed wash VBN 10_1101-2021_01_08_425864 128 8 twice twice RB 10_1101-2021_01_08_425864 128 9 in in IN 10_1101-2021_01_08_425864 128 10 PBS PBS NNP 10_1101-2021_01_08_425864 128 11 and and CC 10_1101-2021_01_08_425864 128 12 incubated incubate VBD 10_1101-2021_01_08_425864 128 13 in in IN 10_1101-2021_01_08_425864 128 14 4 4 CD 10_1101-2021_01_08_425864 128 15 % % NN 10_1101-2021_01_08_425864 128 16 paraformaldehyde paraformaldehyde NN 10_1101-2021_01_08_425864 128 17 for for IN 10_1101-2021_01_08_425864 128 18 15 15 CD 10_1101-2021_01_08_425864 128 19 min min NN 10_1101-2021_01_08_425864 128 20 . . . 10_1101-2021_01_08_425864 129 1 After after IN 10_1101-2021_01_08_425864 129 2 .CC .CC NFP 10_1101-2021_01_08_425864 129 3 - - : 10_1101-2021_01_08_425864 129 4 BY by IN 10_1101-2021_01_08_425864 129 5 4.0 4.0 CD 10_1101-2021_01_08_425864 129 6 International international JJ 10_1101-2021_01_08_425864 129 7 licensemade licensemade NN 10_1101-2021_01_08_425864 129 8 available available JJ 10_1101-2021_01_08_425864 129 9 under under IN 10_1101-2021_01_08_425864 129 10 a a DT 10_1101-2021_01_08_425864 129 11 ( ( -LRB- 10_1101-2021_01_08_425864 129 12 which which WDT 10_1101-2021_01_08_425864 129 13 was be VBD 10_1101-2021_01_08_425864 129 14 not not RB 10_1101-2021_01_08_425864 129 15 certified certify VBN 10_1101-2021_01_08_425864 129 16 by by IN 10_1101-2021_01_08_425864 129 17 peer peer NN 10_1101-2021_01_08_425864 129 18 review review NN 10_1101-2021_01_08_425864 129 19 ) ) -RRB- 10_1101-2021_01_08_425864 129 20 is be VBZ 10_1101-2021_01_08_425864 129 21 the the DT 10_1101-2021_01_08_425864 129 22 author author NN 10_1101-2021_01_08_425864 129 23 / / SYM 10_1101-2021_01_08_425864 129 24 funder funder NN 10_1101-2021_01_08_425864 129 25 , , , 10_1101-2021_01_08_425864 129 26 who who WP 10_1101-2021_01_08_425864 129 27 has have VBZ 10_1101-2021_01_08_425864 129 28 granted grant VBN 10_1101-2021_01_08_425864 129 29 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 129 30 a a DT 10_1101-2021_01_08_425864 129 31 license license NN 10_1101-2021_01_08_425864 129 32 to to TO 10_1101-2021_01_08_425864 129 33 display display VB 10_1101-2021_01_08_425864 129 34 the the DT 10_1101-2021_01_08_425864 129 35 preprint preprint NN 10_1101-2021_01_08_425864 129 36 in in IN 10_1101-2021_01_08_425864 129 37 perpetuity perpetuity NN 10_1101-2021_01_08_425864 129 38 . . . 10_1101-2021_01_08_425864 130 1 It -PRON- PRP 10_1101-2021_01_08_425864 130 2 is be VBZ 10_1101-2021_01_08_425864 130 3 The the DT 10_1101-2021_01_08_425864 130 4 copyright copyright NN 10_1101-2021_01_08_425864 130 5 holder holder NN 10_1101-2021_01_08_425864 130 6 for for IN 10_1101-2021_01_08_425864 130 7 this this DT 10_1101-2021_01_08_425864 130 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 130 9 version version NN 10_1101-2021_01_08_425864 130 10 posted post VBD 10_1101-2021_01_08_425864 130 11 January January NNP 10_1101-2021_01_08_425864 130 12 8 8 CD 10_1101-2021_01_08_425864 130 13 , , , 10_1101-2021_01_08_425864 130 14 2021 2021 CD 10_1101-2021_01_08_425864 130 15 . . . 10_1101-2021_01_08_425864 130 16 ; ; : 10_1101-2021_01_08_425864 130 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 130 18 : : : 10_1101-2021_01_08_425864 130 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 130 20 preprint preprint NN 10_1101-2021_01_08_425864 130 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 130 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ VBZ 10_1101-2021_01_08_425864 130 23 237 237 CD 10_1101-2021_01_08_425864 130 24 incubation incubation NN 10_1101-2021_01_08_425864 130 25 , , , 10_1101-2021_01_08_425864 130 26 the the DT 10_1101-2021_01_08_425864 130 27 cells cell NNS 10_1101-2021_01_08_425864 130 28 were be VBD 10_1101-2021_01_08_425864 130 29 washed wash VBN 10_1101-2021_01_08_425864 130 30 twice twice RB 10_1101-2021_01_08_425864 130 31 with with IN 10_1101-2021_01_08_425864 130 32 PBS PBS NNP 10_1101-2021_01_08_425864 130 33 and and CC 10_1101-2021_01_08_425864 130 34 suspended suspend VBN 10_1101-2021_01_08_425864 130 35 in in IN 10_1101-2021_01_08_425864 130 36 the the DT 10_1101-2021_01_08_425864 130 37 same same JJ 10_1101-2021_01_08_425864 130 38 buffer buffer NN 10_1101-2021_01_08_425864 130 39 . . . 10_1101-2021_01_08_425864 131 1 Flow flow VB 10_1101-2021_01_08_425864 131 2 cytometry cytometry NN 10_1101-2021_01_08_425864 131 3 238 238 CD 10_1101-2021_01_08_425864 131 4 analysis analysis NN 10_1101-2021_01_08_425864 131 5 was be VBD 10_1101-2021_01_08_425864 131 6 performed perform VBN 10_1101-2021_01_08_425864 131 7 with with IN 10_1101-2021_01_08_425864 131 8 FL-1 FL-1 NNP 10_1101-2021_01_08_425864 131 9 and and CC 10_1101-2021_01_08_425864 131 10 FL-2 FL-2 NNP 10_1101-2021_01_08_425864 131 11 filters filter NNS 10_1101-2021_01_08_425864 131 12 in in IN 10_1101-2021_01_08_425864 131 13 a a DT 10_1101-2021_01_08_425864 131 14 FACS FACS NNP 10_1101-2021_01_08_425864 131 15 Fortessa Fortessa NNP 10_1101-2021_01_08_425864 131 16 DB DB NNP 10_1101-2021_01_08_425864 131 17 ® ® NNP 10_1101-2021_01_08_425864 131 18 . . . 10_1101-2021_01_08_425864 132 1 The the DT 10_1101-2021_01_08_425864 132 2 results result NNS 10_1101-2021_01_08_425864 132 3 were be VBD 10_1101-2021_01_08_425864 132 4 239 239 CD 10_1101-2021_01_08_425864 132 5 analysed analyse VBN 10_1101-2021_01_08_425864 132 6 using use VBG 10_1101-2021_01_08_425864 132 7 FloJo FloJo NNP 10_1101-2021_01_08_425864 132 8 software software NN 10_1101-2021_01_08_425864 132 9 . . . 10_1101-2021_01_08_425864 133 1 240 240 CD 10_1101-2021_01_08_425864 133 2 241 241 CD 10_1101-2021_01_08_425864 133 3 Fluorescence fluorescence NN 10_1101-2021_01_08_425864 133 4 microscopy microscopy NN 10_1101-2021_01_08_425864 133 5 242 242 CD 10_1101-2021_01_08_425864 133 6 The the DT 10_1101-2021_01_08_425864 133 7 parasites parasite NNS 10_1101-2021_01_08_425864 133 8 were be VBD 10_1101-2021_01_08_425864 133 9 maintained maintain VBN 10_1101-2021_01_08_425864 133 10 in in IN 10_1101-2021_01_08_425864 133 11 LIT LIT NNP 10_1101-2021_01_08_425864 133 12 medium medium NN 10_1101-2021_01_08_425864 133 13 as as IN 10_1101-2021_01_08_425864 133 14 previously previously RB 10_1101-2021_01_08_425864 133 15 reported report VBN 10_1101-2021_01_08_425864 133 16 for for IN 10_1101-2021_01_08_425864 133 17 fatty fatty NN 10_1101-2021_01_08_425864 133 18 acid acid NN 10_1101-2021_01_08_425864 133 19 staining stain VBG 10_1101-2021_01_08_425864 133 20 243 243 CD 10_1101-2021_01_08_425864 133 21 using use VBG 10_1101-2021_01_08_425864 133 22 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 133 23 ® ® NNPS 10_1101-2021_01_08_425864 133 24 500/510 500/510 NNP 10_1101-2021_01_08_425864 133 25 . . . 10_1101-2021_01_08_425864 134 1 After after IN 10_1101-2021_01_08_425864 134 2 incubation incubation NN 10_1101-2021_01_08_425864 134 3 , , , 10_1101-2021_01_08_425864 134 4 the the DT 10_1101-2021_01_08_425864 134 5 cells cell NNS 10_1101-2021_01_08_425864 134 6 were be VBD 10_1101-2021_01_08_425864 134 7 washed wash VBN 10_1101-2021_01_08_425864 134 8 twice twice RB 10_1101-2021_01_08_425864 134 9 in in IN 10_1101-2021_01_08_425864 134 10 PBS PBS NNP 10_1101-2021_01_08_425864 134 11 and and CC 10_1101-2021_01_08_425864 134 12 placed place VBN 10_1101-2021_01_08_425864 134 13 on on IN 10_1101-2021_01_08_425864 134 14 glass glass NN 10_1101-2021_01_08_425864 134 15 244 244 CD 10_1101-2021_01_08_425864 134 16 slides slide NNS 10_1101-2021_01_08_425864 134 17 . . . 10_1101-2021_01_08_425864 135 1 The the DT 10_1101-2021_01_08_425864 135 2 images image NNS 10_1101-2021_01_08_425864 135 3 were be VBD 10_1101-2021_01_08_425864 135 4 acquired acquire VBN 10_1101-2021_01_08_425864 135 5 with with IN 10_1101-2021_01_08_425864 135 6 a a DT 10_1101-2021_01_08_425864 135 7 digital digital JJ 10_1101-2021_01_08_425864 135 8 DFC DFC NNP 10_1101-2021_01_08_425864 135 9 365 365 CD 10_1101-2021_01_08_425864 135 10 FX FX NNP 10_1101-2021_01_08_425864 135 11 camera camera NN 10_1101-2021_01_08_425864 135 12 coupled couple VBN 10_1101-2021_01_08_425864 135 13 to to IN 10_1101-2021_01_08_425864 135 14 a a DT 10_1101-2021_01_08_425864 135 15 DMI6000B dmi6000b NN 10_1101-2021_01_08_425864 135 16 / / SYM 10_1101-2021_01_08_425864 135 17 AF6000 AF6000 NNP 10_1101-2021_01_08_425864 135 18 245 245 CD 10_1101-2021_01_08_425864 135 19 microscope microscope NN 10_1101-2021_01_08_425864 135 20 ( ( -LRB- 10_1101-2021_01_08_425864 135 21 Leica Leica NNP 10_1101-2021_01_08_425864 135 22 ) ) -RRB- 10_1101-2021_01_08_425864 135 23 . . . 10_1101-2021_01_08_425864 136 1 The the DT 10_1101-2021_01_08_425864 136 2 images image NNS 10_1101-2021_01_08_425864 136 3 were be VBD 10_1101-2021_01_08_425864 136 4 analysed analyse VBN 10_1101-2021_01_08_425864 136 5 using use VBG 10_1101-2021_01_08_425864 136 6 ImageJ ImageJ NNPS 10_1101-2021_01_08_425864 136 7 software software NN 10_1101-2021_01_08_425864 136 8 . . . 10_1101-2021_01_08_425864 137 1 246 246 CD 10_1101-2021_01_08_425864 137 2 247 247 CD 10_1101-2021_01_08_425864 137 3 Results result NNS 10_1101-2021_01_08_425864 137 4 248 248 CD 10_1101-2021_01_08_425864 137 5 Palmitate Palmitate NNP 10_1101-2021_01_08_425864 137 6 supports support VBZ 10_1101-2021_01_08_425864 137 7 ATP atp NN 10_1101-2021_01_08_425864 137 8 synthesis synthesis NN 10_1101-2021_01_08_425864 137 9 in in IN 10_1101-2021_01_08_425864 137 10 T. t. NN 10_1101-2021_01_08_425864 137 11 cruzi cruzi NNS 10_1101-2021_01_08_425864 137 12 249 249 CD 10_1101-2021_01_08_425864 137 13 We -PRON- PRP 10_1101-2021_01_08_425864 137 14 initially initially RB 10_1101-2021_01_08_425864 137 15 investigated investigate VBD 10_1101-2021_01_08_425864 137 16 the the DT 10_1101-2021_01_08_425864 137 17 ability ability NN 10_1101-2021_01_08_425864 137 18 of of IN 10_1101-2021_01_08_425864 137 19 T. T. NNP 10_1101-2021_01_08_425864 137 20 cruzi cruzi NNS 10_1101-2021_01_08_425864 137 21 epimastigotes epimastigote VBZ 10_1101-2021_01_08_425864 137 22 to to TO 10_1101-2021_01_08_425864 137 23 oxidize oxidize VB 10_1101-2021_01_08_425864 137 24 fatty fatty NN 10_1101-2021_01_08_425864 137 25 acids acid NNS 10_1101-2021_01_08_425864 137 26 . . . 10_1101-2021_01_08_425864 138 1 To to IN 10_1101-2021_01_08_425864 138 2 this this DT 10_1101-2021_01_08_425864 138 3 250 250 CD 10_1101-2021_01_08_425864 138 4 end end NN 10_1101-2021_01_08_425864 138 5 , , , 10_1101-2021_01_08_425864 138 6 we -PRON- PRP 10_1101-2021_01_08_425864 138 7 used use VBD 10_1101-2021_01_08_425864 138 8 palmitate palmitate NN 10_1101-2021_01_08_425864 138 9 as as IN 10_1101-2021_01_08_425864 138 10 a a DT 10_1101-2021_01_08_425864 138 11 proxy proxy NN 10_1101-2021_01_08_425864 138 12 for for IN 10_1101-2021_01_08_425864 138 13 fatty fatty NN 10_1101-2021_01_08_425864 138 14 acids acid NNS 10_1101-2021_01_08_425864 138 15 in in IN 10_1101-2021_01_08_425864 138 16 general general JJ 10_1101-2021_01_08_425864 138 17 . . . 10_1101-2021_01_08_425864 139 1 The the DT 10_1101-2021_01_08_425864 139 2 parasites parasite NNS 10_1101-2021_01_08_425864 139 3 were be VBD 10_1101-2021_01_08_425864 139 4 incubated incubate VBN 10_1101-2021_01_08_425864 139 5 with with IN 10_1101-2021_01_08_425864 139 6 0.1 0.1 CD 10_1101-2021_01_08_425864 139 7 251 251 CD 10_1101-2021_01_08_425864 139 8 mM mM NNP 10_1101-2021_01_08_425864 139 9 14C-[U]-palmitate 14c-[u]-palmitate NN 10_1101-2021_01_08_425864 139 10 , , , 10_1101-2021_01_08_425864 139 11 which which WDT 10_1101-2021_01_08_425864 139 12 allowed allow VBD 10_1101-2021_01_08_425864 139 13 us -PRON- PRP 10_1101-2021_01_08_425864 139 14 to to TO 10_1101-2021_01_08_425864 139 15 measure measure VB 10_1101-2021_01_08_425864 139 16 the the DT 10_1101-2021_01_08_425864 139 17 production production NN 10_1101-2021_01_08_425864 139 18 of of IN 10_1101-2021_01_08_425864 139 19 1.3 1.3 CD 10_1101-2021_01_08_425864 139 20 nmoles nmole NNS 10_1101-2021_01_08_425864 139 21 of of IN 10_1101-2021_01_08_425864 139 22 CO2 CO2 NNP 10_1101-2021_01_08_425864 139 23 derived derive VBD 10_1101-2021_01_08_425864 139 24 252 252 CD 10_1101-2021_01_08_425864 139 25 from from IN 10_1101-2021_01_08_425864 139 26 palmitate palmitate NN 10_1101-2021_01_08_425864 139 27 oxidation oxidation NN 10_1101-2021_01_08_425864 139 28 during during IN 10_1101-2021_01_08_425864 139 29 the the DT 10_1101-2021_01_08_425864 139 30 first first JJ 10_1101-2021_01_08_425864 139 31 60 60 CD 10_1101-2021_01_08_425864 139 32 min min NN 10_1101-2021_01_08_425864 139 33 and and CC 10_1101-2021_01_08_425864 139 34 1.5 1.5 CD 10_1101-2021_01_08_425864 139 35 extra extra JJ 10_1101-2021_01_08_425864 139 36 nmoles nmole NNS 10_1101-2021_01_08_425864 139 37 during during IN 10_1101-2021_01_08_425864 139 38 the the DT 10_1101-2021_01_08_425864 139 39 following follow VBG 10_1101-2021_01_08_425864 139 40 60 60 CD 10_1101-2021_01_08_425864 139 41 min min NN 10_1101-2021_01_08_425864 139 42 253 253 CD 10_1101-2021_01_08_425864 139 43 ( ( -LRB- 10_1101-2021_01_08_425864 139 44 Fig Fig NNP 10_1101-2021_01_08_425864 139 45 1a 1a NNP 10_1101-2021_01_08_425864 139 46 ) ) -RRB- 10_1101-2021_01_08_425864 139 47 . . . 10_1101-2021_01_08_425864 140 1 This this DT 10_1101-2021_01_08_425864 140 2 finding finding NN 10_1101-2021_01_08_425864 140 3 indicated indicate VBD 10_1101-2021_01_08_425864 140 4 that that IN 10_1101-2021_01_08_425864 140 5 beta beta NN 10_1101-2021_01_08_425864 140 6 - - HYPH 10_1101-2021_01_08_425864 140 7 oxidation oxidation NN 10_1101-2021_01_08_425864 140 8 and and CC 10_1101-2021_01_08_425864 140 9 the the DT 10_1101-2021_01_08_425864 140 10 further further RB 10_1101-2021_01_08_425864 140 11 ‘ ' `` 10_1101-2021_01_08_425864 140 12 burning burn VBG 10_1101-2021_01_08_425864 140 13 ’ ' '' 10_1101-2021_01_08_425864 140 14 of of IN 10_1101-2021_01_08_425864 140 15 the the DT 10_1101-2021_01_08_425864 140 16 resulting result VBG 10_1101-2021_01_08_425864 140 17 acetyl- acetyl- JJ 10_1101-2021_01_08_425864 140 18 254 254 CD 10_1101-2021_01_08_425864 140 19 CoA CoA NNP 10_1101-2021_01_08_425864 140 20 is be VBZ 10_1101-2021_01_08_425864 140 21 operative operative JJ 10_1101-2021_01_08_425864 140 22 in in IN 10_1101-2021_01_08_425864 140 23 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 140 24 mitochondria mitochondria NNP 10_1101-2021_01_08_425864 140 25 . . . 10_1101-2021_01_08_425864 141 1 Because because IN 10_1101-2021_01_08_425864 141 2 palmitate palmitate NN 10_1101-2021_01_08_425864 141 3 is be VBZ 10_1101-2021_01_08_425864 141 4 taken take VBN 10_1101-2021_01_08_425864 141 5 up up RP 10_1101-2021_01_08_425864 141 6 from from IN 10_1101-2021_01_08_425864 141 7 extracellular extracellular NNP 10_1101-2021_01_08_425864 141 8 255 255 CD 10_1101-2021_01_08_425864 141 9 medium medium NN 10_1101-2021_01_08_425864 141 10 and and CC 10_1101-2021_01_08_425864 141 11 oxidized oxidize VBN 10_1101-2021_01_08_425864 141 12 to to IN 10_1101-2021_01_08_425864 141 13 CO2 CO2 NNP 10_1101-2021_01_08_425864 141 14 , , , 10_1101-2021_01_08_425864 141 15 it -PRON- PRP 10_1101-2021_01_08_425864 141 16 is be VBZ 10_1101-2021_01_08_425864 141 17 reasonable reasonable JJ 10_1101-2021_01_08_425864 141 18 to to TO 10_1101-2021_01_08_425864 141 19 assume assume VB 10_1101-2021_01_08_425864 141 20 that that IN 10_1101-2021_01_08_425864 141 21 it -PRON- PRP 10_1101-2021_01_08_425864 141 22 could could MD 10_1101-2021_01_08_425864 141 23 contribute contribute VB 10_1101-2021_01_08_425864 141 24 to to IN 10_1101-2021_01_08_425864 141 25 resistance resistance NN 10_1101-2021_01_08_425864 141 26 to to IN 10_1101-2021_01_08_425864 141 27 severe severe JJ 10_1101-2021_01_08_425864 141 28 256 256 CD 10_1101-2021_01_08_425864 141 29 nutritional nutritional JJ 10_1101-2021_01_08_425864 141 30 stress stress NN 10_1101-2021_01_08_425864 141 31 . . . 10_1101-2021_01_08_425864 142 1 To to TO 10_1101-2021_01_08_425864 142 2 support support VB 10_1101-2021_01_08_425864 142 3 this this DT 10_1101-2021_01_08_425864 142 4 idea idea NN 10_1101-2021_01_08_425864 142 5 , , , 10_1101-2021_01_08_425864 142 6 we -PRON- PRP 10_1101-2021_01_08_425864 142 7 tested test VBD 10_1101-2021_01_08_425864 142 8 the the DT 10_1101-2021_01_08_425864 142 9 ability ability NN 10_1101-2021_01_08_425864 142 10 of of IN 10_1101-2021_01_08_425864 142 11 palmitate palmitate NN 10_1101-2021_01_08_425864 142 12 to to TO 10_1101-2021_01_08_425864 142 13 extend extend VB 10_1101-2021_01_08_425864 142 14 parasite parasite NN 10_1101-2021_01_08_425864 142 15 survival survival NN 10_1101-2021_01_08_425864 142 16 257 257 CD 10_1101-2021_01_08_425864 142 17 under under IN 10_1101-2021_01_08_425864 142 18 extreme extreme JJ 10_1101-2021_01_08_425864 142 19 nutritional nutritional JJ 10_1101-2021_01_08_425864 142 20 stress stress NN 10_1101-2021_01_08_425864 142 21 . . . 10_1101-2021_01_08_425864 143 1 Parasites parasite NNS 10_1101-2021_01_08_425864 143 2 were be VBD 10_1101-2021_01_08_425864 143 3 incubated incubate VBN 10_1101-2021_01_08_425864 143 4 for for IN 10_1101-2021_01_08_425864 143 5 24 24 CD 10_1101-2021_01_08_425864 143 6 and and CC 10_1101-2021_01_08_425864 143 7 48 48 CD 10_1101-2021_01_08_425864 143 8 h h NNS 10_1101-2021_01_08_425864 143 9 in in IN 10_1101-2021_01_08_425864 143 10 PBS PBS NNP 10_1101-2021_01_08_425864 143 11 ( ( -LRB- 10_1101-2021_01_08_425864 143 12 negative negative JJ 10_1101-2021_01_08_425864 143 13 control control NN 10_1101-2021_01_08_425864 143 14 , , , 10_1101-2021_01_08_425864 143 15 258 258 CD 10_1101-2021_01_08_425864 143 16 in in IN 10_1101-2021_01_08_425864 143 17 this this DT 10_1101-2021_01_08_425864 143 18 condition condition NN 10_1101-2021_01_08_425864 143 19 we -PRON- PRP 10_1101-2021_01_08_425864 143 20 expected expect VBD 10_1101-2021_01_08_425864 143 21 the the DT 10_1101-2021_01_08_425864 143 22 lower low JJR 10_1101-2021_01_08_425864 143 23 viability viability NN 10_1101-2021_01_08_425864 143 24 after after IN 10_1101-2021_01_08_425864 143 25 the the DT 10_1101-2021_01_08_425864 143 26 incubations incubation NNS 10_1101-2021_01_08_425864 143 27 ) ) -RRB- 10_1101-2021_01_08_425864 143 28 , , , 10_1101-2021_01_08_425864 143 29 0.1 0.1 CD 10_1101-2021_01_08_425864 143 30 mM mm NN 10_1101-2021_01_08_425864 143 31 palmitate palmitate NN 10_1101-2021_01_08_425864 143 32 in in IN 10_1101-2021_01_08_425864 143 33 PBS PBS NNP 10_1101-2021_01_08_425864 143 34 259 259 CD 10_1101-2021_01_08_425864 143 35 supplemented supplement VBD 10_1101-2021_01_08_425864 143 36 with with IN 10_1101-2021_01_08_425864 143 37 BSA BSA NNP 10_1101-2021_01_08_425864 143 38 ( ( -LRB- 10_1101-2021_01_08_425864 143 39 as as IN 10_1101-2021_01_08_425864 143 40 a a DT 10_1101-2021_01_08_425864 143 41 palmitate palmitate NN 10_1101-2021_01_08_425864 143 42 carrier carrier NN 10_1101-2021_01_08_425864 143 43 ) ) -RRB- 10_1101-2021_01_08_425864 143 44 , , , 10_1101-2021_01_08_425864 143 45 5.0 5.0 CD 10_1101-2021_01_08_425864 143 46 mM mm NN 10_1101-2021_01_08_425864 143 47 histidine histidine NN 10_1101-2021_01_08_425864 143 48 in in IN 10_1101-2021_01_08_425864 143 49 PBS PBS NNP 10_1101-2021_01_08_425864 143 50 or or CC 10_1101-2021_01_08_425864 143 51 5.0 5.0 CD 10_1101-2021_01_08_425864 143 52 mM mm NN 10_1101-2021_01_08_425864 143 53 glucose glucose NN 10_1101-2021_01_08_425864 143 54 in in IN 10_1101-2021_01_08_425864 143 55 PBS PBS NNP 10_1101-2021_01_08_425864 143 56 260 260 CD 10_1101-2021_01_08_425864 143 57 ( ( -LRB- 10_1101-2021_01_08_425864 143 58 both both CC 10_1101-2021_01_08_425864 143 59 positive positive JJ 10_1101-2021_01_08_425864 143 60 controls control NNS 10_1101-2021_01_08_425864 143 61 , , , 10_1101-2021_01_08_425864 143 62 since since IN 10_1101-2021_01_08_425864 143 63 it -PRON- PRP 10_1101-2021_01_08_425864 143 64 is be VBZ 10_1101-2021_01_08_425864 143 65 well well RB 10_1101-2021_01_08_425864 143 66 knowing know VBG 10_1101-2021_01_08_425864 143 67 the the DT 10_1101-2021_01_08_425864 143 68 ability ability NN 10_1101-2021_01_08_425864 143 69 of of IN 10_1101-2021_01_08_425864 143 70 both both DT 10_1101-2021_01_08_425864 143 71 metabolites metabolite NNS 10_1101-2021_01_08_425864 143 72 to to TO 10_1101-2021_01_08_425864 143 73 extend extend VB 10_1101-2021_01_08_425864 143 74 the the DT 10_1101-2021_01_08_425864 143 75 parasites parasite NNS 10_1101-2021_01_08_425864 143 76 ´ ´ CD 10_1101-2021_01_08_425864 143 77 261 261 CD 10_1101-2021_01_08_425864 143 78 viability viability NN 10_1101-2021_01_08_425864 143 79 in in IN 10_1101-2021_01_08_425864 143 80 metabolic metabolic JJ 10_1101-2021_01_08_425864 143 81 stress stress NN 10_1101-2021_01_08_425864 143 82 conditions condition NNS 10_1101-2021_01_08_425864 143 83 , , , 10_1101-2021_01_08_425864 143 84 see see VB 10_1101-2021_01_08_425864 143 85 [ [ -LRB- 10_1101-2021_01_08_425864 143 86 15 15 CD 10_1101-2021_01_08_425864 143 87 ] ] -RRB- 10_1101-2021_01_08_425864 143 88 ) ) -RRB- 10_1101-2021_01_08_425864 143 89 . . . 10_1101-2021_01_08_425864 144 1 As as IN 10_1101-2021_01_08_425864 144 2 an an DT 10_1101-2021_01_08_425864 144 3 additional additional JJ 10_1101-2021_01_08_425864 144 4 negative negative JJ 10_1101-2021_01_08_425864 144 5 control control NN 10_1101-2021_01_08_425864 144 6 , , , 10_1101-2021_01_08_425864 144 7 we -PRON- PRP 10_1101-2021_01_08_425864 144 8 used use VBD 10_1101-2021_01_08_425864 144 9 PBS PBS NNP 10_1101-2021_01_08_425864 144 10 262 262 CD 10_1101-2021_01_08_425864 144 11 supplemented supplement VBD 10_1101-2021_01_08_425864 144 12 with with IN 10_1101-2021_01_08_425864 144 13 BSA BSA NNP 10_1101-2021_01_08_425864 144 14 without without IN 10_1101-2021_01_08_425864 144 15 added add VBN 10_1101-2021_01_08_425864 144 16 palmitate palmitate NN 10_1101-2021_01_08_425864 144 17 . . . 10_1101-2021_01_08_425864 145 1 The the DT 10_1101-2021_01_08_425864 145 2 viability viability NN 10_1101-2021_01_08_425864 145 3 of of IN 10_1101-2021_01_08_425864 145 4 these these DT 10_1101-2021_01_08_425864 145 5 cells cell NNS 10_1101-2021_01_08_425864 145 6 was be VBD 10_1101-2021_01_08_425864 145 7 assayed assay VBN 10_1101-2021_01_08_425864 145 8 by by IN 10_1101-2021_01_08_425864 145 9 263 263 CD 10_1101-2021_01_08_425864 145 10 measuring measure VBG 10_1101-2021_01_08_425864 145 11 the the DT 10_1101-2021_01_08_425864 145 12 total total JJ 10_1101-2021_01_08_425864 145 13 reductive reductive JJ 10_1101-2021_01_08_425864 145 14 activity activity NN 10_1101-2021_01_08_425864 145 15 by by IN 10_1101-2021_01_08_425864 145 16 MTT MTT NNP 10_1101-2021_01_08_425864 145 17 assay assay NNP 10_1101-2021_01_08_425864 145 18 . . . 10_1101-2021_01_08_425864 146 1 Additionally additionally RB 10_1101-2021_01_08_425864 146 2 , , , 10_1101-2021_01_08_425864 146 3 we -PRON- PRP 10_1101-2021_01_08_425864 146 4 measured measure VBD 10_1101-2021_01_08_425864 146 5 the the DT 10_1101-2021_01_08_425864 146 6 total total JJ 10_1101-2021_01_08_425864 146 7 ATP ATP NNP 10_1101-2021_01_08_425864 146 8 levels level NNS 10_1101-2021_01_08_425864 146 9 . . . 10_1101-2021_01_08_425864 147 1 264 264 CD 10_1101-2021_01_08_425864 147 2 Cells cell NNS 10_1101-2021_01_08_425864 147 3 incubated incubate VBD 10_1101-2021_01_08_425864 147 4 in in IN 10_1101-2021_01_08_425864 147 5 the the DT 10_1101-2021_01_08_425864 147 6 presence presence NN 10_1101-2021_01_08_425864 147 7 of of IN 10_1101-2021_01_08_425864 147 8 palmitate palmitate NN 10_1101-2021_01_08_425864 147 9 showed show VBD 10_1101-2021_01_08_425864 147 10 higher high JJR 10_1101-2021_01_08_425864 147 11 viability viability NN 10_1101-2021_01_08_425864 147 12 than than IN 10_1101-2021_01_08_425864 147 13 the the DT 10_1101-2021_01_08_425864 147 14 negative negative JJ 10_1101-2021_01_08_425864 147 15 controls control NNS 10_1101-2021_01_08_425864 147 16 , , , 10_1101-2021_01_08_425864 147 17 but but CC 10_1101-2021_01_08_425864 147 18 265 265 CD 10_1101-2021_01_08_425864 147 19 not not RB 10_1101-2021_01_08_425864 147 20 as as RB 10_1101-2021_01_08_425864 147 21 high high JJ 10_1101-2021_01_08_425864 147 22 as as IN 10_1101-2021_01_08_425864 147 23 that that DT 10_1101-2021_01_08_425864 147 24 of of IN 10_1101-2021_01_08_425864 147 25 parasites parasite NNS 10_1101-2021_01_08_425864 147 26 incubated incubate VBN 10_1101-2021_01_08_425864 147 27 with with IN 10_1101-2021_01_08_425864 147 28 glucose glucose NN 10_1101-2021_01_08_425864 147 29 or or CC 10_1101-2021_01_08_425864 147 30 histidine histidine NN 10_1101-2021_01_08_425864 147 31 ( ( -LRB- 10_1101-2021_01_08_425864 147 32 Fig fig NN 10_1101-2021_01_08_425864 147 33 1b 1b CD 10_1101-2021_01_08_425864 147 34 ) ) -RRB- 10_1101-2021_01_08_425864 147 35 . . . 10_1101-2021_01_08_425864 148 1 Consistently consistently RB 10_1101-2021_01_08_425864 148 2 , , , 10_1101-2021_01_08_425864 148 3 parasites parasite NNS 10_1101-2021_01_08_425864 148 4 266 266 CD 10_1101-2021_01_08_425864 148 5 incubated incubate VBD 10_1101-2021_01_08_425864 148 6 in in IN 10_1101-2021_01_08_425864 148 7 the the DT 10_1101-2021_01_08_425864 148 8 presence presence NN 10_1101-2021_01_08_425864 148 9 of of IN 10_1101-2021_01_08_425864 148 10 palmitate palmitate NN 10_1101-2021_01_08_425864 148 11 showed show VBD 10_1101-2021_01_08_425864 148 12 higher high JJR 10_1101-2021_01_08_425864 148 13 ATP atp NN 10_1101-2021_01_08_425864 148 14 contents content NNS 10_1101-2021_01_08_425864 148 15 than than IN 10_1101-2021_01_08_425864 148 16 both both DT 10_1101-2021_01_08_425864 148 17 negative negative JJ 10_1101-2021_01_08_425864 148 18 controls control NNS 10_1101-2021_01_08_425864 148 19 . . . 10_1101-2021_01_08_425864 149 1 267 267 CD 10_1101-2021_01_08_425864 149 2 However however RB 10_1101-2021_01_08_425864 149 3 , , , 10_1101-2021_01_08_425864 149 4 the the DT 10_1101-2021_01_08_425864 149 5 intracellular intracellular JJ 10_1101-2021_01_08_425864 149 6 ATP ATP NNP 10_1101-2021_01_08_425864 149 7 levels level NNS 10_1101-2021_01_08_425864 149 8 in in IN 10_1101-2021_01_08_425864 149 9 the the DT 10_1101-2021_01_08_425864 149 10 cells cell NNS 10_1101-2021_01_08_425864 149 11 incubated incubate VBN 10_1101-2021_01_08_425864 149 12 with with IN 10_1101-2021_01_08_425864 149 13 palmitate palmitate NN 10_1101-2021_01_08_425864 149 14 were be VBD 10_1101-2021_01_08_425864 149 15 diminished diminish VBN 10_1101-2021_01_08_425864 149 16 by by IN 10_1101-2021_01_08_425864 149 17 half half NN 10_1101-2021_01_08_425864 149 18 268 268 CD 10_1101-2021_01_08_425864 149 19 when when WRB 10_1101-2021_01_08_425864 149 20 compared compare VBN 10_1101-2021_01_08_425864 149 21 to to IN 10_1101-2021_01_08_425864 149 22 parasites parasite NNS 10_1101-2021_01_08_425864 149 23 incubated incubate VBN 10_1101-2021_01_08_425864 149 24 with with IN 10_1101-2021_01_08_425864 149 25 histidine histidine NN 10_1101-2021_01_08_425864 149 26 . . . 10_1101-2021_01_08_425864 150 1 Interestingly interestingly RB 10_1101-2021_01_08_425864 150 2 , , , 10_1101-2021_01_08_425864 150 3 the the DT 10_1101-2021_01_08_425864 150 4 palmitate palmitate NN 10_1101-2021_01_08_425864 150 5 kept keep VBD 10_1101-2021_01_08_425864 150 6 the the DT 10_1101-2021_01_08_425864 150 7 ATP ATP NNP 10_1101-2021_01_08_425864 150 8 269 269 CD 10_1101-2021_01_08_425864 150 9 content content NN 10_1101-2021_01_08_425864 150 10 at at IN 10_1101-2021_01_08_425864 150 11 levels level NNS 10_1101-2021_01_08_425864 150 12 comparable comparable JJ 10_1101-2021_01_08_425864 150 13 to to TO 10_1101-2021_01_08_425864 150 14 glucose glucose VB 10_1101-2021_01_08_425864 150 15 ( ( -LRB- 10_1101-2021_01_08_425864 150 16 Fig fig NN 10_1101-2021_01_08_425864 150 17 1c 1c CD 10_1101-2021_01_08_425864 150 18 ) ) -RRB- 10_1101-2021_01_08_425864 150 19 . . . 10_1101-2021_01_08_425864 151 1 270 270 CD 10_1101-2021_01_08_425864 151 2 .CC .CC : 10_1101-2021_01_08_425864 151 3 - - : 10_1101-2021_01_08_425864 151 4 BY by IN 10_1101-2021_01_08_425864 151 5 4.0 4.0 CD 10_1101-2021_01_08_425864 151 6 International international JJ 10_1101-2021_01_08_425864 151 7 licensemade licensemade NN 10_1101-2021_01_08_425864 151 8 available available JJ 10_1101-2021_01_08_425864 151 9 under under IN 10_1101-2021_01_08_425864 151 10 a a DT 10_1101-2021_01_08_425864 151 11 ( ( -LRB- 10_1101-2021_01_08_425864 151 12 which which WDT 10_1101-2021_01_08_425864 151 13 was be VBD 10_1101-2021_01_08_425864 151 14 not not RB 10_1101-2021_01_08_425864 151 15 certified certify VBN 10_1101-2021_01_08_425864 151 16 by by IN 10_1101-2021_01_08_425864 151 17 peer peer NN 10_1101-2021_01_08_425864 151 18 review review NN 10_1101-2021_01_08_425864 151 19 ) ) -RRB- 10_1101-2021_01_08_425864 151 20 is be VBZ 10_1101-2021_01_08_425864 151 21 the the DT 10_1101-2021_01_08_425864 151 22 author author NN 10_1101-2021_01_08_425864 151 23 / / SYM 10_1101-2021_01_08_425864 151 24 funder funder NN 10_1101-2021_01_08_425864 151 25 , , , 10_1101-2021_01_08_425864 151 26 who who WP 10_1101-2021_01_08_425864 151 27 has have VBZ 10_1101-2021_01_08_425864 151 28 granted grant VBN 10_1101-2021_01_08_425864 151 29 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 151 30 a a DT 10_1101-2021_01_08_425864 151 31 license license NN 10_1101-2021_01_08_425864 151 32 to to TO 10_1101-2021_01_08_425864 151 33 display display VB 10_1101-2021_01_08_425864 151 34 the the DT 10_1101-2021_01_08_425864 151 35 preprint preprint NN 10_1101-2021_01_08_425864 151 36 in in IN 10_1101-2021_01_08_425864 151 37 perpetuity perpetuity NN 10_1101-2021_01_08_425864 151 38 . . . 10_1101-2021_01_08_425864 152 1 It -PRON- PRP 10_1101-2021_01_08_425864 152 2 is be VBZ 10_1101-2021_01_08_425864 152 3 The the DT 10_1101-2021_01_08_425864 152 4 copyright copyright NN 10_1101-2021_01_08_425864 152 5 holder holder NN 10_1101-2021_01_08_425864 152 6 for for IN 10_1101-2021_01_08_425864 152 7 this this DT 10_1101-2021_01_08_425864 152 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 152 9 version version NN 10_1101-2021_01_08_425864 152 10 posted post VBD 10_1101-2021_01_08_425864 152 11 January January NNP 10_1101-2021_01_08_425864 152 12 8 8 CD 10_1101-2021_01_08_425864 152 13 , , , 10_1101-2021_01_08_425864 152 14 2021 2021 CD 10_1101-2021_01_08_425864 152 15 . . . 10_1101-2021_01_08_425864 152 16 ; ; : 10_1101-2021_01_08_425864 152 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 152 18 : : : 10_1101-2021_01_08_425864 152 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 152 20 preprint preprint NN 10_1101-2021_01_08_425864 152 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 152 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 152 23 271 271 CD 10_1101-2021_01_08_425864 152 24 Figure figure NN 10_1101-2021_01_08_425864 152 25 1 1 CD 10_1101-2021_01_08_425864 152 26 . . . 10_1101-2021_01_08_425864 153 1 Palmitate palmitate NN 10_1101-2021_01_08_425864 153 2 oxidation oxidation NN 10_1101-2021_01_08_425864 153 3 promotes promote VBZ 10_1101-2021_01_08_425864 153 4 ATP atp NN 10_1101-2021_01_08_425864 153 5 production production NN 10_1101-2021_01_08_425864 153 6 and and CC 10_1101-2021_01_08_425864 153 7 viability viability NN 10_1101-2021_01_08_425864 153 8 in in IN 10_1101-2021_01_08_425864 153 9 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 153 10 forms form NNS 10_1101-2021_01_08_425864 153 11 272 272 CD 10_1101-2021_01_08_425864 153 12 under under IN 10_1101-2021_01_08_425864 153 13 starvation starvation NN 10_1101-2021_01_08_425864 153 14 . . . 10_1101-2021_01_08_425864 154 1 Schematic schematic JJ 10_1101-2021_01_08_425864 154 2 representation representation NN 10_1101-2021_01_08_425864 154 3 of of IN 10_1101-2021_01_08_425864 154 4 14C 14c CD 10_1101-2021_01_08_425864 154 5 - - HYPH 10_1101-2021_01_08_425864 154 6 U U NNP 10_1101-2021_01_08_425864 154 7 - - HYPH 10_1101-2021_01_08_425864 154 8 palmitate palmitate NN 10_1101-2021_01_08_425864 154 9 metabolism metabolism NN 10_1101-2021_01_08_425864 154 10 . . . 10_1101-2021_01_08_425864 155 1 The the DT 10_1101-2021_01_08_425864 155 2 metabolites metabolite NNS 10_1101-2021_01_08_425864 155 3 273 273 CD 10_1101-2021_01_08_425864 155 4 corresponding correspond VBG 10_1101-2021_01_08_425864 155 5 to to IN 10_1101-2021_01_08_425864 155 6 labelled label VBN 10_1101-2021_01_08_425864 155 7 palmitate palmitate NN 10_1101-2021_01_08_425864 155 8 metabolism metabolism NN 10_1101-2021_01_08_425864 155 9 are be VBP 10_1101-2021_01_08_425864 155 10 presented present VBN 10_1101-2021_01_08_425864 155 11 in in IN 10_1101-2021_01_08_425864 155 12 green green NN 10_1101-2021_01_08_425864 155 13 . . . 10_1101-2021_01_08_425864 156 1 A a DT 10_1101-2021_01_08_425864 156 2 ) ) -RRB- 10_1101-2021_01_08_425864 156 3 14CO2 14co2 CD 10_1101-2021_01_08_425864 156 4 production production NN 10_1101-2021_01_08_425864 156 5 from from IN 10_1101-2021_01_08_425864 156 6 274 274 CD 10_1101-2021_01_08_425864 156 7 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 156 8 incubated incubate VBN 10_1101-2021_01_08_425864 156 9 in in IN 10_1101-2021_01_08_425864 156 10 PBS PBS NNP 10_1101-2021_01_08_425864 156 11 with with IN 10_1101-2021_01_08_425864 156 12 14C 14c CD 10_1101-2021_01_08_425864 156 13 - - HYPH 10_1101-2021_01_08_425864 156 14 U U NNP 10_1101-2021_01_08_425864 156 15 - - HYPH 10_1101-2021_01_08_425864 156 16 palmitate palmitate NNP 10_1101-2021_01_08_425864 156 17 100 100 CD 10_1101-2021_01_08_425864 156 18 µM. µM. NNS 10_1101-2021_01_08_425864 157 1 The the DT 10_1101-2021_01_08_425864 157 2 14CO2 14co2 CD 10_1101-2021_01_08_425864 157 3 was be VBD 10_1101-2021_01_08_425864 157 4 captured capture VBN 10_1101-2021_01_08_425864 157 5 at at IN 10_1101-2021_01_08_425864 157 6 0 0 CD 10_1101-2021_01_08_425864 157 7 , , , 10_1101-2021_01_08_425864 157 8 30 30 CD 10_1101-2021_01_08_425864 157 9 , , , 10_1101-2021_01_08_425864 157 10 60 60 CD 10_1101-2021_01_08_425864 157 11 275 275 CD 10_1101-2021_01_08_425864 157 12 and and CC 10_1101-2021_01_08_425864 157 13 120 120 CD 10_1101-2021_01_08_425864 157 14 min min NN 10_1101-2021_01_08_425864 157 15 . . . 10_1101-2021_01_08_425864 158 1 B b NN 10_1101-2021_01_08_425864 158 2 ) ) -RRB- 10_1101-2021_01_08_425864 158 3 Viability viability NN 10_1101-2021_01_08_425864 158 4 of of IN 10_1101-2021_01_08_425864 158 5 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 158 6 forms form NNS 10_1101-2021_01_08_425864 158 7 after after IN 10_1101-2021_01_08_425864 158 8 incubation incubation NN 10_1101-2021_01_08_425864 158 9 with with IN 10_1101-2021_01_08_425864 158 10 different different JJ 10_1101-2021_01_08_425864 158 11 carbon carbon NN 10_1101-2021_01_08_425864 158 12 sources source NNS 10_1101-2021_01_08_425864 158 13 and and CC 10_1101-2021_01_08_425864 158 14 276 276 CD 10_1101-2021_01_08_425864 158 15 palmitate palmitate NN 10_1101-2021_01_08_425864 158 16 . . . 10_1101-2021_01_08_425864 159 1 The the DT 10_1101-2021_01_08_425864 159 2 viability viability NN 10_1101-2021_01_08_425864 159 3 was be VBD 10_1101-2021_01_08_425864 159 4 assessed assess VBN 10_1101-2021_01_08_425864 159 5 after after IN 10_1101-2021_01_08_425864 159 6 24 24 CD 10_1101-2021_01_08_425864 159 7 and and CC 10_1101-2021_01_08_425864 159 8 48 48 CD 10_1101-2021_01_08_425864 159 9 h h NN 10_1101-2021_01_08_425864 159 10 by by IN 10_1101-2021_01_08_425864 159 11 MTT MTT NNP 10_1101-2021_01_08_425864 159 12 assay assay NNP 10_1101-2021_01_08_425864 159 13 . . . 10_1101-2021_01_08_425864 160 1 C C NNP 10_1101-2021_01_08_425864 160 2 ) ) -RRB- 10_1101-2021_01_08_425864 160 3 The the DT 10_1101-2021_01_08_425864 160 4 intracellular intracellular JJ 10_1101-2021_01_08_425864 160 5 ATP atp NN 10_1101-2021_01_08_425864 160 6 277 277 CD 10_1101-2021_01_08_425864 160 7 content content NN 10_1101-2021_01_08_425864 160 8 was be VBD 10_1101-2021_01_08_425864 160 9 evaluated evaluate VBN 10_1101-2021_01_08_425864 160 10 following follow VBG 10_1101-2021_01_08_425864 160 11 incubation incubation NN 10_1101-2021_01_08_425864 160 12 with with IN 10_1101-2021_01_08_425864 160 13 different different JJ 10_1101-2021_01_08_425864 160 14 energy energy NN 10_1101-2021_01_08_425864 160 15 substrates substrate NNS 10_1101-2021_01_08_425864 160 16 or or CC 10_1101-2021_01_08_425864 160 17 not not RB 10_1101-2021_01_08_425864 160 18 ( ( -LRB- 10_1101-2021_01_08_425864 160 19 PBS PBS NNP 10_1101-2021_01_08_425864 160 20 , , , 10_1101-2021_01_08_425864 160 21 negative negative JJ 10_1101-2021_01_08_425864 160 22 278 278 CD 10_1101-2021_01_08_425864 160 23 control control NN 10_1101-2021_01_08_425864 160 24 ) ) -RRB- 10_1101-2021_01_08_425864 160 25 . . . 10_1101-2021_01_08_425864 161 1 The the DT 10_1101-2021_01_08_425864 161 2 ATP ATP NNP 10_1101-2021_01_08_425864 161 3 concentration concentration NN 10_1101-2021_01_08_425864 161 4 was be VBD 10_1101-2021_01_08_425864 161 5 determined determine VBN 10_1101-2021_01_08_425864 161 6 by by IN 10_1101-2021_01_08_425864 161 7 luciferase luciferase NN 10_1101-2021_01_08_425864 161 8 assay assay NNP 10_1101-2021_01_08_425864 161 9 and and CC 10_1101-2021_01_08_425864 161 10 the the DT 10_1101-2021_01_08_425864 161 11 data datum NNS 10_1101-2021_01_08_425864 161 12 were be VBD 10_1101-2021_01_08_425864 161 13 adjusted adjust VBN 10_1101-2021_01_08_425864 161 14 by by IN 10_1101-2021_01_08_425864 161 15 279 279 CD 10_1101-2021_01_08_425864 161 16 the the DT 10_1101-2021_01_08_425864 161 17 number number NN 10_1101-2021_01_08_425864 161 18 of of IN 10_1101-2021_01_08_425864 161 19 cells cell NNS 10_1101-2021_01_08_425864 161 20 . . . 10_1101-2021_01_08_425864 162 1 A a DT 10_1101-2021_01_08_425864 162 2 statistical statistical JJ 10_1101-2021_01_08_425864 162 3 analysis analysis NN 10_1101-2021_01_08_425864 162 4 was be VBD 10_1101-2021_01_08_425864 162 5 performed perform VBN 10_1101-2021_01_08_425864 162 6 with with IN 10_1101-2021_01_08_425864 162 7 one one CD 10_1101-2021_01_08_425864 162 8 - - HYPH 10_1101-2021_01_08_425864 162 9 way way NN 10_1101-2021_01_08_425864 162 10 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 162 11 followed follow VBN 10_1101-2021_01_08_425864 162 12 by by IN 10_1101-2021_01_08_425864 162 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 162 14 's 's POS 10_1101-2021_01_08_425864 162 15 280 280 CD 10_1101-2021_01_08_425864 162 16 post post NN 10_1101-2021_01_08_425864 162 17 - - NN 10_1101-2021_01_08_425864 162 18 test test NN 10_1101-2021_01_08_425864 162 19 at at IN 10_1101-2021_01_08_425864 162 20 p p NN 10_1101-2021_01_08_425864 162 21 < < XX 10_1101-2021_01_08_425864 162 22 0.05 0.05 XX 10_1101-2021_01_08_425864 162 23 using use VBG 10_1101-2021_01_08_425864 162 24 the the DT 10_1101-2021_01_08_425864 162 25 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 162 26 Prism Prism NNP 10_1101-2021_01_08_425864 162 27 8.0.2 8.0.2 NNP 10_1101-2021_01_08_425864 162 28 software software NN 10_1101-2021_01_08_425864 162 29 program program NN 10_1101-2021_01_08_425864 162 30 . . . 10_1101-2021_01_08_425864 163 1 We -PRON- PRP 10_1101-2021_01_08_425864 163 2 represent represent VBP 10_1101-2021_01_08_425864 163 3 the the DT 10_1101-2021_01_08_425864 163 4 level level NN 10_1101-2021_01_08_425864 163 5 of of IN 10_1101-2021_01_08_425864 163 6 281 281 CD 10_1101-2021_01_08_425864 163 7 statistical statistical JJ 10_1101-2021_01_08_425864 163 8 significance significance NN 10_1101-2021_01_08_425864 163 9 in in IN 10_1101-2021_01_08_425864 163 10 this this DT 10_1101-2021_01_08_425864 163 11 figure figure NN 10_1101-2021_01_08_425864 163 12 as as IN 10_1101-2021_01_08_425864 163 13 follows follow VBZ 10_1101-2021_01_08_425864 163 14 : : : 10_1101-2021_01_08_425864 163 15 * * NFP 10_1101-2021_01_08_425864 163 16 * * NFP 10_1101-2021_01_08_425864 163 17 * * NFP 10_1101-2021_01_08_425864 163 18 p p NN 10_1101-2021_01_08_425864 163 19 value value NN 10_1101-2021_01_08_425864 163 20 < < XX 10_1101-2021_01_08_425864 163 21 0.001 0.001 CD 10_1101-2021_01_08_425864 163 22 ; ; : 10_1101-2021_01_08_425864 163 23 * * NFP 10_1101-2021_01_08_425864 163 24 * * NFP 10_1101-2021_01_08_425864 163 25 p p NN 10_1101-2021_01_08_425864 163 26 value value NN 10_1101-2021_01_08_425864 163 27 < < XX 10_1101-2021_01_08_425864 163 28 0.01 0.01 XX 10_1101-2021_01_08_425864 163 29 ; ; : 10_1101-2021_01_08_425864 163 30 * * NFP 10_1101-2021_01_08_425864 163 31 p p NN 10_1101-2021_01_08_425864 163 32 value value NN 10_1101-2021_01_08_425864 163 33 < < XX 10_1101-2021_01_08_425864 163 34 282 282 CD 10_1101-2021_01_08_425864 163 35 0.05 0.05 CD 10_1101-2021_01_08_425864 163 36 . . . 10_1101-2021_01_08_425864 164 1 For for IN 10_1101-2021_01_08_425864 164 2 a a DT 10_1101-2021_01_08_425864 164 3 p p NN 10_1101-2021_01_08_425864 164 4 value value NN 10_1101-2021_01_08_425864 164 5 > > NN 10_1101-2021_01_08_425864 164 6 0.05 0.05 CD 10_1101-2021_01_08_425864 164 7 we -PRON- PRP 10_1101-2021_01_08_425864 164 8 consider consider VBP 10_1101-2021_01_08_425864 164 9 the the DT 10_1101-2021_01_08_425864 164 10 differences difference NNS 10_1101-2021_01_08_425864 164 11 to to TO 10_1101-2021_01_08_425864 164 12 be be VB 10_1101-2021_01_08_425864 164 13 not not RB 10_1101-2021_01_08_425864 164 14 significant significant JJ 10_1101-2021_01_08_425864 164 15 ( ( -LRB- 10_1101-2021_01_08_425864 164 16 ns ns NNP 10_1101-2021_01_08_425864 164 17 ) ) -RRB- 10_1101-2021_01_08_425864 164 18 . . . 10_1101-2021_01_08_425864 165 1 283 283 CD 10_1101-2021_01_08_425864 165 2 284 284 CD 10_1101-2021_01_08_425864 165 3 Epimastigote epimastigote JJ 10_1101-2021_01_08_425864 165 4 forms form NNS 10_1101-2021_01_08_425864 165 5 excrete excrete VBP 10_1101-2021_01_08_425864 165 6 acetate acetate NNP 10_1101-2021_01_08_425864 165 7 as as IN 10_1101-2021_01_08_425864 165 8 a a DT 10_1101-2021_01_08_425864 165 9 primary primary JJ 10_1101-2021_01_08_425864 165 10 end end NN 10_1101-2021_01_08_425864 165 11 - - HYPH 10_1101-2021_01_08_425864 165 12 product product NN 10_1101-2021_01_08_425864 165 13 of of IN 10_1101-2021_01_08_425864 165 14 palmitate palmitate NN 10_1101-2021_01_08_425864 165 15 oxidation oxidation NN 10_1101-2021_01_08_425864 165 16 285 285 CD 10_1101-2021_01_08_425864 165 17 Because because IN 10_1101-2021_01_08_425864 165 18 the the DT 10_1101-2021_01_08_425864 165 19 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 165 20 were be VBD 10_1101-2021_01_08_425864 165 21 able able JJ 10_1101-2021_01_08_425864 165 22 to to TO 10_1101-2021_01_08_425864 165 23 oxidize oxidize VB 10_1101-2021_01_08_425864 165 24 14C 14c CD 10_1101-2021_01_08_425864 165 25 - - HYPH 10_1101-2021_01_08_425864 165 26 U u NN 10_1101-2021_01_08_425864 165 27 - - HYPH 10_1101-2021_01_08_425864 165 28 palmitate palmitate NN 10_1101-2021_01_08_425864 165 29 to to IN 10_1101-2021_01_08_425864 165 30 14CO2 14co2 CD 10_1101-2021_01_08_425864 165 31 , , , 10_1101-2021_01_08_425864 165 32 we -PRON- PRP 10_1101-2021_01_08_425864 165 33 were be VBD 10_1101-2021_01_08_425864 165 34 interested interested JJ 10_1101-2021_01_08_425864 165 35 286 286 CD 10_1101-2021_01_08_425864 165 36 in in IN 10_1101-2021_01_08_425864 165 37 analysing analyse VBG 10_1101-2021_01_08_425864 165 38 their -PRON- PRP$ 10_1101-2021_01_08_425864 165 39 exometabolome exometabolome NN 10_1101-2021_01_08_425864 165 40 and and CC 10_1101-2021_01_08_425864 165 41 comparing compare VBG 10_1101-2021_01_08_425864 165 42 it -PRON- PRP 10_1101-2021_01_08_425864 165 43 with with IN 10_1101-2021_01_08_425864 165 44 that that DT 10_1101-2021_01_08_425864 165 45 of of IN 10_1101-2021_01_08_425864 165 46 parasites parasite NNS 10_1101-2021_01_08_425864 165 47 exclusively exclusively RB 10_1101-2021_01_08_425864 165 48 consuming consume VBG 10_1101-2021_01_08_425864 165 49 287 287 CD 10_1101-2021_01_08_425864 165 50 glucose glucose NN 10_1101-2021_01_08_425864 165 51 , , , 10_1101-2021_01_08_425864 165 52 palmitate palmitate VBP 10_1101-2021_01_08_425864 165 53 or or CC 10_1101-2021_01_08_425864 165 54 without without IN 10_1101-2021_01_08_425864 165 55 any any DT 10_1101-2021_01_08_425864 165 56 carbon carbon NN 10_1101-2021_01_08_425864 165 57 source source NN 10_1101-2021_01_08_425864 165 58 . . . 10_1101-2021_01_08_425864 166 1 Thus thus RB 10_1101-2021_01_08_425864 166 2 , , , 10_1101-2021_01_08_425864 166 3 we -PRON- PRP 10_1101-2021_01_08_425864 166 4 subjected subject VBD 10_1101-2021_01_08_425864 166 5 exponentially exponentially RB 10_1101-2021_01_08_425864 166 6 growing grow VBG 10_1101-2021_01_08_425864 166 7 parasites parasite NNS 10_1101-2021_01_08_425864 166 8 288 288 CD 10_1101-2021_01_08_425864 166 9 to to TO 10_1101-2021_01_08_425864 166 10 16 16 CD 10_1101-2021_01_08_425864 166 11 h h NN 10_1101-2021_01_08_425864 166 12 of of IN 10_1101-2021_01_08_425864 166 13 starvation starvation NN 10_1101-2021_01_08_425864 166 14 and and CC 10_1101-2021_01_08_425864 166 15 then then RB 10_1101-2021_01_08_425864 166 16 incubated incubate VBD 10_1101-2021_01_08_425864 166 17 them -PRON- PRP 10_1101-2021_01_08_425864 166 18 for for IN 10_1101-2021_01_08_425864 166 19 6 6 CD 10_1101-2021_01_08_425864 166 20 h h NN 10_1101-2021_01_08_425864 166 21 in in IN 10_1101-2021_01_08_425864 166 22 the the DT 10_1101-2021_01_08_425864 166 23 presence presence NN 10_1101-2021_01_08_425864 166 24 of of IN 10_1101-2021_01_08_425864 166 25 0.3 0.3 CD 10_1101-2021_01_08_425864 166 26 mM mM NNP 10_1101-2021_01_08_425864 166 27 palmitate palmitate NN 10_1101-2021_01_08_425864 166 28 , , , 10_1101-2021_01_08_425864 166 29 10 10 CD 10_1101-2021_01_08_425864 166 30 mM mm NN 10_1101-2021_01_08_425864 166 31 289 289 CD 10_1101-2021_01_08_425864 166 32 13C 13c CD 10_1101-2021_01_08_425864 166 33 - - HYPH 10_1101-2021_01_08_425864 166 34 U u NN 10_1101-2021_01_08_425864 166 35 - - HYPH 10_1101-2021_01_08_425864 166 36 glucose glucose NN 10_1101-2021_01_08_425864 166 37 or or CC 10_1101-2021_01_08_425864 166 38 without without IN 10_1101-2021_01_08_425864 166 39 any any DT 10_1101-2021_01_08_425864 166 40 carbon carbon NN 10_1101-2021_01_08_425864 166 41 source source NN 10_1101-2021_01_08_425864 166 42 . . . 10_1101-2021_01_08_425864 167 1 For for IN 10_1101-2021_01_08_425864 167 2 the the DT 10_1101-2021_01_08_425864 167 3 control control NN 10_1101-2021_01_08_425864 167 4 , , , 10_1101-2021_01_08_425864 167 5 we -PRON- PRP 10_1101-2021_01_08_425864 167 6 analysed analyse VBD 10_1101-2021_01_08_425864 167 7 a a DT 10_1101-2021_01_08_425864 167 8 sample sample NN 10_1101-2021_01_08_425864 167 9 of of IN 10_1101-2021_01_08_425864 167 10 non non JJ 10_1101-2021_01_08_425864 167 11 - - JJ 10_1101-2021_01_08_425864 167 12 starved starve VBN 10_1101-2021_01_08_425864 167 13 290 290 CD 10_1101-2021_01_08_425864 167 14 parasites parasite NNS 10_1101-2021_01_08_425864 167 15 . . . 10_1101-2021_01_08_425864 168 1 After after IN 10_1101-2021_01_08_425864 168 2 the the DT 10_1101-2021_01_08_425864 168 3 incubations incubation NNS 10_1101-2021_01_08_425864 168 4 , , , 10_1101-2021_01_08_425864 168 5 the the DT 10_1101-2021_01_08_425864 168 6 extracellular extracellular JJ 10_1101-2021_01_08_425864 168 7 media medium NNS 10_1101-2021_01_08_425864 168 8 were be VBD 10_1101-2021_01_08_425864 168 9 collected collect VBN 10_1101-2021_01_08_425864 168 10 and and CC 10_1101-2021_01_08_425864 168 11 analysed analyse VBN 10_1101-2021_01_08_425864 168 12 by by IN 10_1101-2021_01_08_425864 168 13 1H 1h JJ 10_1101-2021_01_08_425864 168 14 - - : 10_1101-2021_01_08_425864 168 15 NMR NMR NNP 10_1101-2021_01_08_425864 168 16 291 291 CD 10_1101-2021_01_08_425864 168 17 spectrometry spectrometry NN 10_1101-2021_01_08_425864 168 18 . . . 10_1101-2021_01_08_425864 169 1 As as IN 10_1101-2021_01_08_425864 169 2 expected expect VBN 10_1101-2021_01_08_425864 169 3 , , , 10_1101-2021_01_08_425864 169 4 all all PDT 10_1101-2021_01_08_425864 169 5 the the DT 10_1101-2021_01_08_425864 169 6 incubation incubation NN 10_1101-2021_01_08_425864 169 7 conditions condition NNS 10_1101-2021_01_08_425864 169 8 produced produce VBD 10_1101-2021_01_08_425864 169 9 different different JJ 10_1101-2021_01_08_425864 169 10 flux flux JJ 10_1101-2021_01_08_425864 169 11 profiles profile NNS 10_1101-2021_01_08_425864 169 12 for for IN 10_1101-2021_01_08_425864 169 13 excreted excreted JJ 10_1101-2021_01_08_425864 169 14 292 292 CD 10_1101-2021_01_08_425864 169 15 metabolites metabolite NNS 10_1101-2021_01_08_425864 169 16 ( ( -LRB- 10_1101-2021_01_08_425864 169 17 Fig fig NN 10_1101-2021_01_08_425864 169 18 2 2 CD 10_1101-2021_01_08_425864 169 19 and and CC 10_1101-2021_01_08_425864 169 20 S1 S1 NNP 10_1101-2021_01_08_425864 169 21 Fig Fig NNP 10_1101-2021_01_08_425864 169 22 ) ) -RRB- 10_1101-2021_01_08_425864 169 23 . . . 10_1101-2021_01_08_425864 170 1 Under under IN 10_1101-2021_01_08_425864 170 2 our -PRON- PRP$ 10_1101-2021_01_08_425864 170 3 experimental experimental JJ 10_1101-2021_01_08_425864 170 4 conditions condition NNS 10_1101-2021_01_08_425864 170 5 , , , 10_1101-2021_01_08_425864 170 6 the the DT 10_1101-2021_01_08_425864 170 7 non non JJ 10_1101-2021_01_08_425864 170 8 - - JJ 10_1101-2021_01_08_425864 170 9 starved starved JJ 10_1101-2021_01_08_425864 170 10 parasites parasite NNS 10_1101-2021_01_08_425864 170 11 293 293 CD 10_1101-2021_01_08_425864 170 12 primarily primarily RB 10_1101-2021_01_08_425864 170 13 excreted excrete VBN 10_1101-2021_01_08_425864 170 14 succinate succinate NN 10_1101-2021_01_08_425864 170 15 and and CC 10_1101-2021_01_08_425864 170 16 acetate acetate JJ 10_1101-2021_01_08_425864 170 17 in in IN 10_1101-2021_01_08_425864 170 18 similar similar JJ 10_1101-2021_01_08_425864 170 19 quantities quantity NNS 10_1101-2021_01_08_425864 170 20 , , , 10_1101-2021_01_08_425864 170 21 and and CC 10_1101-2021_01_08_425864 170 22 alanine alanine NN 10_1101-2021_01_08_425864 170 23 and and CC 10_1101-2021_01_08_425864 170 24 lactate lactate VB 10_1101-2021_01_08_425864 170 25 to to IN 10_1101-2021_01_08_425864 170 26 a a DT 10_1101-2021_01_08_425864 170 27 lesser less JJR 10_1101-2021_01_08_425864 170 28 extent extent NN 10_1101-2021_01_08_425864 170 29 . . . 10_1101-2021_01_08_425864 171 1 294 294 CD 10_1101-2021_01_08_425864 171 2 Parasites Parasites NNPS 10_1101-2021_01_08_425864 171 3 starved starve VBD 10_1101-2021_01_08_425864 171 4 for for IN 10_1101-2021_01_08_425864 171 5 16 16 CD 10_1101-2021_01_08_425864 171 6 h h NN 10_1101-2021_01_08_425864 171 7 in in IN 10_1101-2021_01_08_425864 171 8 PBS PBS NNP 10_1101-2021_01_08_425864 171 9 and and CC 10_1101-2021_01_08_425864 171 10 left leave VBD 10_1101-2021_01_08_425864 171 11 to to TO 10_1101-2021_01_08_425864 171 12 incubate incubate VB 10_1101-2021_01_08_425864 171 13 in in IN 10_1101-2021_01_08_425864 171 14 the the DT 10_1101-2021_01_08_425864 171 15 absence absence NN 10_1101-2021_01_08_425864 171 16 of of IN 10_1101-2021_01_08_425864 171 17 other other JJ 10_1101-2021_01_08_425864 171 18 metabolites metabolite NNS 10_1101-2021_01_08_425864 171 19 had have VBD 10_1101-2021_01_08_425864 171 20 295 295 CD 10_1101-2021_01_08_425864 171 21 diminished diminish VBN 10_1101-2021_01_08_425864 171 22 succinate succinate NN 10_1101-2021_01_08_425864 171 23 production production NN 10_1101-2021_01_08_425864 171 24 ( ( -LRB- 10_1101-2021_01_08_425864 171 25 ~7-fold ~7-fold NN 10_1101-2021_01_08_425864 171 26 ) ) -RRB- 10_1101-2021_01_08_425864 171 27 but but CC 10_1101-2021_01_08_425864 171 28 increased increase VBN 10_1101-2021_01_08_425864 171 29 acetate acetate JJ 10_1101-2021_01_08_425864 171 30 production production NN 10_1101-2021_01_08_425864 171 31 three three CD 10_1101-2021_01_08_425864 171 32 - - HYPH 10_1101-2021_01_08_425864 171 33 fold fold RB 10_1101-2021_01_08_425864 171 34 compared compare VBN 10_1101-2021_01_08_425864 171 35 to to IN 10_1101-2021_01_08_425864 171 36 296 296 CD 10_1101-2021_01_08_425864 171 37 the the DT 10_1101-2021_01_08_425864 171 38 non non JJ 10_1101-2021_01_08_425864 171 39 - - JJ 10_1101-2021_01_08_425864 171 40 starved starved JJ 10_1101-2021_01_08_425864 171 41 parasites parasite NNS 10_1101-2021_01_08_425864 171 42 . . . 10_1101-2021_01_08_425864 172 1 It -PRON- PRP 10_1101-2021_01_08_425864 172 2 is be VBZ 10_1101-2021_01_08_425864 172 3 relevant relevant JJ 10_1101-2021_01_08_425864 172 4 to to TO 10_1101-2021_01_08_425864 172 5 stress stress VB 10_1101-2021_01_08_425864 172 6 that that IN 10_1101-2021_01_08_425864 172 7 the the DT 10_1101-2021_01_08_425864 172 8 only only JJ 10_1101-2021_01_08_425864 172 9 possible possible JJ 10_1101-2021_01_08_425864 172 10 origin origin NN 10_1101-2021_01_08_425864 172 11 for for IN 10_1101-2021_01_08_425864 172 12 these these DT 10_1101-2021_01_08_425864 172 13 metabolites metabolite NNS 10_1101-2021_01_08_425864 172 14 are be VBP 10_1101-2021_01_08_425864 172 15 297 297 CD 10_1101-2021_01_08_425864 172 16 internal internal JJ 10_1101-2021_01_08_425864 172 17 carbon carbon NN 10_1101-2021_01_08_425864 172 18 sources source NNS 10_1101-2021_01_08_425864 172 19 ( ( -LRB- 10_1101-2021_01_08_425864 172 20 ICS ics NN 10_1101-2021_01_08_425864 172 21 ) ) -RRB- 10_1101-2021_01_08_425864 172 22 . . . 10_1101-2021_01_08_425864 173 1 Notably notably RB 10_1101-2021_01_08_425864 173 2 , , , 10_1101-2021_01_08_425864 173 3 no no DT 10_1101-2021_01_08_425864 173 4 other other JJ 10_1101-2021_01_08_425864 173 5 excreted excreted JJ 10_1101-2021_01_08_425864 173 6 metabolites metabolite NNS 10_1101-2021_01_08_425864 173 7 were be VBD 10_1101-2021_01_08_425864 173 8 detected detect VBN 10_1101-2021_01_08_425864 173 9 under under IN 10_1101-2021_01_08_425864 173 10 these these DT 10_1101-2021_01_08_425864 173 11 298 298 CD 10_1101-2021_01_08_425864 173 12 conditions condition NNS 10_1101-2021_01_08_425864 173 13 , , , 10_1101-2021_01_08_425864 173 14 indicating indicate VBG 10_1101-2021_01_08_425864 173 15 that that IN 10_1101-2021_01_08_425864 173 16 under under IN 10_1101-2021_01_08_425864 173 17 starvation starvation NN 10_1101-2021_01_08_425864 173 18 , , , 10_1101-2021_01_08_425864 173 19 most most JJS 10_1101-2021_01_08_425864 173 20 of of IN 10_1101-2021_01_08_425864 173 21 the the DT 10_1101-2021_01_08_425864 173 22 ICS ICS NNP 10_1101-2021_01_08_425864 173 23 are be VBP 10_1101-2021_01_08_425864 173 24 transformed transform VBN 10_1101-2021_01_08_425864 173 25 into into IN 10_1101-2021_01_08_425864 173 26 acetate acetate NNP 10_1101-2021_01_08_425864 173 27 as as IN 10_1101-2021_01_08_425864 173 28 an an DT 10_1101-2021_01_08_425864 173 29 end end NN 10_1101-2021_01_08_425864 173 30 299 299 CD 10_1101-2021_01_08_425864 173 31 product product NN 10_1101-2021_01_08_425864 173 32 , , , 10_1101-2021_01_08_425864 173 33 which which WDT 10_1101-2021_01_08_425864 173 34 is be VBZ 10_1101-2021_01_08_425864 173 35 compatible compatible JJ 10_1101-2021_01_08_425864 173 36 with with IN 10_1101-2021_01_08_425864 173 37 the the DT 10_1101-2021_01_08_425864 173 38 oxidation oxidation NN 10_1101-2021_01_08_425864 173 39 of of IN 10_1101-2021_01_08_425864 173 40 internal internal JJ 10_1101-2021_01_08_425864 173 41 fatty fatty NN 10_1101-2021_01_08_425864 173 42 acids acid NNS 10_1101-2021_01_08_425864 173 43 . . . 10_1101-2021_01_08_425864 174 1 These these DT 10_1101-2021_01_08_425864 174 2 results result NNS 10_1101-2021_01_08_425864 174 3 raise raise VBP 10_1101-2021_01_08_425864 174 4 the the DT 10_1101-2021_01_08_425864 174 5 question question NN 10_1101-2021_01_08_425864 174 6 300 300 CD 10_1101-2021_01_08_425864 174 7 about about IN 10_1101-2021_01_08_425864 174 8 the the DT 10_1101-2021_01_08_425864 174 9 metabolic metabolic JJ 10_1101-2021_01_08_425864 174 10 fates fate NNS 10_1101-2021_01_08_425864 174 11 of of IN 10_1101-2021_01_08_425864 174 12 glucose glucose NN 10_1101-2021_01_08_425864 174 13 or or CC 10_1101-2021_01_08_425864 174 14 fatty fatty NN 10_1101-2021_01_08_425864 174 15 acids acid NNS 10_1101-2021_01_08_425864 174 16 in in IN 10_1101-2021_01_08_425864 174 17 previously previously RB 10_1101-2021_01_08_425864 174 18 starved starve VBN 10_1101-2021_01_08_425864 174 19 parasites parasite NNS 10_1101-2021_01_08_425864 174 20 . . . 10_1101-2021_01_08_425864 175 1 Starved starve VBN 10_1101-2021_01_08_425864 175 2 301 301 CD 10_1101-2021_01_08_425864 175 3 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 175 4 that that WDT 10_1101-2021_01_08_425864 175 5 recovered recover VBD 10_1101-2021_01_08_425864 175 6 in in IN 10_1101-2021_01_08_425864 175 7 the the DT 10_1101-2021_01_08_425864 175 8 presence presence NN 10_1101-2021_01_08_425864 175 9 of of IN 10_1101-2021_01_08_425864 175 10 glucose glucose NNP 10_1101-2021_01_08_425864 175 11 exhibited exhibit VBD 10_1101-2021_01_08_425864 175 12 a a DT 10_1101-2021_01_08_425864 175 13 profuse profuse NN 10_1101-2021_01_08_425864 175 14 excretion excretion NN 10_1101-2021_01_08_425864 175 15 of of IN 10_1101-2021_01_08_425864 175 16 succinate succinate NNP 10_1101-2021_01_08_425864 175 17 302 302 CD 10_1101-2021_01_08_425864 175 18 ( ( -LRB- 10_1101-2021_01_08_425864 175 19 450-fold 450-fold CD 10_1101-2021_01_08_425864 175 20 the the DT 10_1101-2021_01_08_425864 175 21 quantity quantity NN 10_1101-2021_01_08_425864 175 22 excreted excrete VBN 10_1101-2021_01_08_425864 175 23 by by IN 10_1101-2021_01_08_425864 175 24 the the DT 10_1101-2021_01_08_425864 175 25 starved starve VBN 10_1101-2021_01_08_425864 175 26 cells cell NNS 10_1101-2021_01_08_425864 175 27 ) ) -RRB- 10_1101-2021_01_08_425864 175 28 and and CC 10_1101-2021_01_08_425864 175 29 roughly roughly RB 10_1101-2021_01_08_425864 175 30 equivalent equivalent JJ 10_1101-2021_01_08_425864 175 31 quantities quantity NNS 10_1101-2021_01_08_425864 175 32 of of IN 10_1101-2021_01_08_425864 175 33 acetate acetate JJ 10_1101-2021_01_08_425864 175 34 303 303 CD 10_1101-2021_01_08_425864 175 35 compared compare VBN 10_1101-2021_01_08_425864 175 36 with with IN 10_1101-2021_01_08_425864 175 37 the the DT 10_1101-2021_01_08_425864 175 38 starved starve VBN 10_1101-2021_01_08_425864 175 39 cells cell NNS 10_1101-2021_01_08_425864 175 40 . . . 10_1101-2021_01_08_425864 176 1 Interestingly interestingly RB 10_1101-2021_01_08_425864 176 2 , , , 10_1101-2021_01_08_425864 176 3 lactate lactate JJ 10_1101-2021_01_08_425864 176 4 and and CC 10_1101-2021_01_08_425864 176 5 alanine alanine NN 10_1101-2021_01_08_425864 176 6 were be VBD 10_1101-2021_01_08_425864 176 7 also also RB 10_1101-2021_01_08_425864 176 8 excreted excrete VBN 10_1101-2021_01_08_425864 176 9 at at IN 10_1101-2021_01_08_425864 176 10 similar similar JJ 10_1101-2021_01_08_425864 176 11 levels level NNS 10_1101-2021_01_08_425864 176 12 . . . 10_1101-2021_01_08_425864 177 1 304 304 CD 10_1101-2021_01_08_425864 177 2 As as IN 10_1101-2021_01_08_425864 177 3 expected expect VBN 10_1101-2021_01_08_425864 177 4 , , , 10_1101-2021_01_08_425864 177 5 the the DT 10_1101-2021_01_08_425864 177 6 recovery recovery NN 10_1101-2021_01_08_425864 177 7 with with IN 10_1101-2021_01_08_425864 177 8 glucose glucose NNP 10_1101-2021_01_08_425864 177 9 produced produce VBD 10_1101-2021_01_08_425864 177 10 an an DT 10_1101-2021_01_08_425864 177 11 increase increase NN 10_1101-2021_01_08_425864 177 12 in in IN 10_1101-2021_01_08_425864 177 13 all all PDT 10_1101-2021_01_08_425864 177 14 the the DT 10_1101-2021_01_08_425864 177 15 secreted secrete VBN 10_1101-2021_01_08_425864 177 16 metabolites metabolite NNS 10_1101-2021_01_08_425864 177 17 . . . 10_1101-2021_01_08_425864 178 1 However however RB 10_1101-2021_01_08_425864 178 2 , , , 10_1101-2021_01_08_425864 178 3 305 305 CD 10_1101-2021_01_08_425864 178 4 analysing analyse VBG 10_1101-2021_01_08_425864 178 5 their -PRON- PRP$ 10_1101-2021_01_08_425864 178 6 distribution distribution NN 10_1101-2021_01_08_425864 178 7 is be VBZ 10_1101-2021_01_08_425864 178 8 a a DT 10_1101-2021_01_08_425864 178 9 reconfiguration reconfiguration NN 10_1101-2021_01_08_425864 178 10 of of IN 10_1101-2021_01_08_425864 178 11 the the DT 10_1101-2021_01_08_425864 178 12 metabolism metabolism NN 10_1101-2021_01_08_425864 178 13 towards towards IN 10_1101-2021_01_08_425864 178 14 a a DT 10_1101-2021_01_08_425864 178 15 majority majority NN 10_1101-2021_01_08_425864 178 16 production production NN 10_1101-2021_01_08_425864 178 17 of of IN 10_1101-2021_01_08_425864 178 18 306 306 CD 10_1101-2021_01_08_425864 178 19 succinate succinate NN 10_1101-2021_01_08_425864 178 20 . . . 10_1101-2021_01_08_425864 179 1 Finally finally RB 10_1101-2021_01_08_425864 179 2 , , , 10_1101-2021_01_08_425864 179 3 in in IN 10_1101-2021_01_08_425864 179 4 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 179 5 incubated incubate VBN 10_1101-2021_01_08_425864 179 6 with with IN 10_1101-2021_01_08_425864 179 7 palmitate palmitate NN 10_1101-2021_01_08_425864 179 8 , , , 10_1101-2021_01_08_425864 179 9 we -PRON- PRP 10_1101-2021_01_08_425864 179 10 observed observe VBD 10_1101-2021_01_08_425864 179 11 an an DT 10_1101-2021_01_08_425864 179 12 increase increase NN 10_1101-2021_01_08_425864 179 13 in in IN 10_1101-2021_01_08_425864 179 14 the the DT 10_1101-2021_01_08_425864 179 15 acetate acetate JJ 10_1101-2021_01_08_425864 179 16 307 307 CD 10_1101-2021_01_08_425864 179 17 and and CC 10_1101-2021_01_08_425864 179 18 alanine alanine NN 10_1101-2021_01_08_425864 179 19 production production NN 10_1101-2021_01_08_425864 179 20 of of IN 10_1101-2021_01_08_425864 179 21 approximately approximately RB 10_1101-2021_01_08_425864 179 22 2.5 2.5 CD 10_1101-2021_01_08_425864 179 23 times time NNS 10_1101-2021_01_08_425864 179 24 to to IN 10_1101-2021_01_08_425864 179 25 the the DT 10_1101-2021_01_08_425864 179 26 levels level NNS 10_1101-2021_01_08_425864 179 27 in in IN 10_1101-2021_01_08_425864 179 28 parasites parasite NNS 10_1101-2021_01_08_425864 179 29 that that WDT 10_1101-2021_01_08_425864 179 30 recovered recover VBD 10_1101-2021_01_08_425864 179 31 in in IN 10_1101-2021_01_08_425864 179 32 the the DT 10_1101-2021_01_08_425864 179 33 308 308 CD 10_1101-2021_01_08_425864 179 34 presence presence NN 10_1101-2021_01_08_425864 179 35 of of IN 10_1101-2021_01_08_425864 179 36 glucose glucose NN 10_1101-2021_01_08_425864 179 37 . . . 10_1101-2021_01_08_425864 180 1 Interestingly interestingly RB 10_1101-2021_01_08_425864 180 2 , , , 10_1101-2021_01_08_425864 180 3 succinate succinate NN 10_1101-2021_01_08_425864 180 4 is be VBZ 10_1101-2021_01_08_425864 180 5 excreted excrete VBN 10_1101-2021_01_08_425864 180 6 in in IN 10_1101-2021_01_08_425864 180 7 a a DT 10_1101-2021_01_08_425864 180 8 smaller small JJR 10_1101-2021_01_08_425864 180 9 quantity quantity NN 10_1101-2021_01_08_425864 180 10 than than IN 10_1101-2021_01_08_425864 180 11 acetate acetate NN 10_1101-2021_01_08_425864 180 12 and and CC 10_1101-2021_01_08_425864 180 13 alanine alanine NN 10_1101-2021_01_08_425864 180 14 , , , 10_1101-2021_01_08_425864 180 15 309 309 CD 10_1101-2021_01_08_425864 180 16 but but CC 10_1101-2021_01_08_425864 180 17 still still RB 10_1101-2021_01_08_425864 180 18 at at IN 10_1101-2021_01_08_425864 180 19 10-fold 10-fold CD 10_1101-2021_01_08_425864 180 20 the the DT 10_1101-2021_01_08_425864 180 21 rate rate NN 10_1101-2021_01_08_425864 180 22 observed observe VBN 10_1101-2021_01_08_425864 180 23 in in IN 10_1101-2021_01_08_425864 180 24 the the DT 10_1101-2021_01_08_425864 180 25 starved starve VBN 10_1101-2021_01_08_425864 180 26 non non JJ 10_1101-2021_01_08_425864 180 27 - - JJ 10_1101-2021_01_08_425864 180 28 recovered recovered JJ 10_1101-2021_01_08_425864 180 29 cells cell NNS 10_1101-2021_01_08_425864 180 30 . . . 10_1101-2021_01_08_425864 181 1 Surprisingly surprisingly RB 10_1101-2021_01_08_425864 181 2 , , , 10_1101-2021_01_08_425864 181 3 there there EX 10_1101-2021_01_08_425864 181 4 was be VBD 10_1101-2021_01_08_425864 181 5 also also RB 10_1101-2021_01_08_425864 181 6 a a DT 10_1101-2021_01_08_425864 181 7 .CC .CC NFP 10_1101-2021_01_08_425864 181 8 - - : 10_1101-2021_01_08_425864 181 9 BY by IN 10_1101-2021_01_08_425864 181 10 4.0 4.0 CD 10_1101-2021_01_08_425864 181 11 International international JJ 10_1101-2021_01_08_425864 181 12 licensemade licensemade NN 10_1101-2021_01_08_425864 181 13 available available JJ 10_1101-2021_01_08_425864 181 14 under under IN 10_1101-2021_01_08_425864 181 15 a a DT 10_1101-2021_01_08_425864 181 16 ( ( -LRB- 10_1101-2021_01_08_425864 181 17 which which WDT 10_1101-2021_01_08_425864 181 18 was be VBD 10_1101-2021_01_08_425864 181 19 not not RB 10_1101-2021_01_08_425864 181 20 certified certify VBN 10_1101-2021_01_08_425864 181 21 by by IN 10_1101-2021_01_08_425864 181 22 peer peer NN 10_1101-2021_01_08_425864 181 23 review review NN 10_1101-2021_01_08_425864 181 24 ) ) -RRB- 10_1101-2021_01_08_425864 181 25 is be VBZ 10_1101-2021_01_08_425864 181 26 the the DT 10_1101-2021_01_08_425864 181 27 author author NN 10_1101-2021_01_08_425864 181 28 / / SYM 10_1101-2021_01_08_425864 181 29 funder funder NN 10_1101-2021_01_08_425864 181 30 , , , 10_1101-2021_01_08_425864 181 31 who who WP 10_1101-2021_01_08_425864 181 32 has have VBZ 10_1101-2021_01_08_425864 181 33 granted grant VBN 10_1101-2021_01_08_425864 181 34 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 181 35 a a DT 10_1101-2021_01_08_425864 181 36 license license NN 10_1101-2021_01_08_425864 181 37 to to TO 10_1101-2021_01_08_425864 181 38 display display VB 10_1101-2021_01_08_425864 181 39 the the DT 10_1101-2021_01_08_425864 181 40 preprint preprint NN 10_1101-2021_01_08_425864 181 41 in in IN 10_1101-2021_01_08_425864 181 42 perpetuity perpetuity NN 10_1101-2021_01_08_425864 181 43 . . . 10_1101-2021_01_08_425864 182 1 It -PRON- PRP 10_1101-2021_01_08_425864 182 2 is be VBZ 10_1101-2021_01_08_425864 182 3 The the DT 10_1101-2021_01_08_425864 182 4 copyright copyright NN 10_1101-2021_01_08_425864 182 5 holder holder NN 10_1101-2021_01_08_425864 182 6 for for IN 10_1101-2021_01_08_425864 182 7 this this DT 10_1101-2021_01_08_425864 182 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 182 9 version version NN 10_1101-2021_01_08_425864 182 10 posted post VBD 10_1101-2021_01_08_425864 182 11 January January NNP 10_1101-2021_01_08_425864 182 12 8 8 CD 10_1101-2021_01_08_425864 182 13 , , , 10_1101-2021_01_08_425864 182 14 2021 2021 CD 10_1101-2021_01_08_425864 182 15 . . . 10_1101-2021_01_08_425864 182 16 ; ; : 10_1101-2021_01_08_425864 182 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 182 18 : : : 10_1101-2021_01_08_425864 182 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 182 20 preprint preprint NN 10_1101-2021_01_08_425864 182 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 182 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 182 23 310 310 CD 10_1101-2021_01_08_425864 182 24 significant significant JJ 10_1101-2021_01_08_425864 182 25 production production NN 10_1101-2021_01_08_425864 182 26 of of IN 10_1101-2021_01_08_425864 182 27 pyruvate pyruvate NN 10_1101-2021_01_08_425864 182 28 ( ( -LRB- 10_1101-2021_01_08_425864 182 29 not not RB 10_1101-2021_01_08_425864 182 30 previously previously RB 10_1101-2021_01_08_425864 182 31 described describe VBN 10_1101-2021_01_08_425864 182 32 in in IN 10_1101-2021_01_08_425864 182 33 the the DT 10_1101-2021_01_08_425864 182 34 literature literature NN 10_1101-2021_01_08_425864 182 35 , , , 10_1101-2021_01_08_425864 182 36 and and CC 10_1101-2021_01_08_425864 182 37 not not RB 10_1101-2021_01_08_425864 182 38 observed observe VBN 10_1101-2021_01_08_425864 182 39 under under IN 10_1101-2021_01_08_425864 182 40 311 311 CD 10_1101-2021_01_08_425864 182 41 any any DT 10_1101-2021_01_08_425864 182 42 other other JJ 10_1101-2021_01_08_425864 182 43 conditions condition NNS 10_1101-2021_01_08_425864 182 44 ) ) -RRB- 10_1101-2021_01_08_425864 182 45 and and CC 10_1101-2021_01_08_425864 182 46 a a DT 10_1101-2021_01_08_425864 182 47 small small JJ 10_1101-2021_01_08_425864 182 48 amount amount NN 10_1101-2021_01_08_425864 182 49 of of IN 10_1101-2021_01_08_425864 182 50 lactate lactate NN 10_1101-2021_01_08_425864 182 51 derived derive VBN 10_1101-2021_01_08_425864 182 52 from from IN 10_1101-2021_01_08_425864 182 53 palmitate palmitate NN 10_1101-2021_01_08_425864 182 54 . . . 10_1101-2021_01_08_425864 183 1 312 312 CD 10_1101-2021_01_08_425864 183 2 313 313 CD 10_1101-2021_01_08_425864 183 3 Figure figure NN 10_1101-2021_01_08_425864 183 4 2 2 CD 10_1101-2021_01_08_425864 183 5 . . . 10_1101-2021_01_08_425864 184 1 Excreted excreted JJ 10_1101-2021_01_08_425864 184 2 end end NN 10_1101-2021_01_08_425864 184 3 products product NNS 10_1101-2021_01_08_425864 184 4 of of IN 10_1101-2021_01_08_425864 184 5 glucose glucose NN 10_1101-2021_01_08_425864 184 6 and and CC 10_1101-2021_01_08_425864 184 7 palmitate palmitate VB 10_1101-2021_01_08_425864 184 8 metabolism metabolism NN 10_1101-2021_01_08_425864 184 9 in in IN 10_1101-2021_01_08_425864 184 10 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 184 11 forms form NNS 10_1101-2021_01_08_425864 184 12 of of IN 10_1101-2021_01_08_425864 184 13 314 314 CD 10_1101-2021_01_08_425864 184 14 T. t. NN 10_1101-2021_01_08_425864 184 15 cruzi cruzi NNS 10_1101-2021_01_08_425864 184 16 . . . 10_1101-2021_01_08_425864 185 1 A a LS 10_1101-2021_01_08_425864 185 2 ) ) -RRB- 10_1101-2021_01_08_425864 185 3 The the DT 10_1101-2021_01_08_425864 185 4 extracellular extracellular JJ 10_1101-2021_01_08_425864 185 5 medium medium NN 10_1101-2021_01_08_425864 185 6 of of IN 10_1101-2021_01_08_425864 185 7 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 185 8 forms form NNS 10_1101-2021_01_08_425864 185 9 incubated incubate VBN 10_1101-2021_01_08_425864 185 10 under under IN 10_1101-2021_01_08_425864 185 11 different different JJ 10_1101-2021_01_08_425864 185 12 conditions condition NNS 10_1101-2021_01_08_425864 185 13 315 315 CD 10_1101-2021_01_08_425864 185 14 was be VBD 10_1101-2021_01_08_425864 185 15 analysed analyse VBN 10_1101-2021_01_08_425864 185 16 by by IN 10_1101-2021_01_08_425864 185 17 1H 1h JJ 10_1101-2021_01_08_425864 185 18 - - HYPH 10_1101-2021_01_08_425864 185 19 NMR NMR NNP 10_1101-2021_01_08_425864 185 20 spectrometry spectrometry NN 10_1101-2021_01_08_425864 185 21 to to TO 10_1101-2021_01_08_425864 185 22 detect detect VB 10_1101-2021_01_08_425864 185 23 and and CC 10_1101-2021_01_08_425864 185 24 quantify quantify VB 10_1101-2021_01_08_425864 185 25 the the DT 10_1101-2021_01_08_425864 185 26 end end NN 10_1101-2021_01_08_425864 185 27 - - HYPH 10_1101-2021_01_08_425864 185 28 products product NNS 10_1101-2021_01_08_425864 185 29 . . . 10_1101-2021_01_08_425864 186 1 The the DT 10_1101-2021_01_08_425864 186 2 resulting result VBG 10_1101-2021_01_08_425864 186 3 data datum NNS 10_1101-2021_01_08_425864 186 4 316 316 CD 10_1101-2021_01_08_425864 186 5 were be VBD 10_1101-2021_01_08_425864 186 6 expressed express VBN 10_1101-2021_01_08_425864 186 7 in in IN 10_1101-2021_01_08_425864 186 8 nmoles nmoles NNP 10_1101-2021_01_08_425864 186 9 / / SYM 10_1101-2021_01_08_425864 186 10 h/108 h/108 NNP 10_1101-2021_01_08_425864 186 11 cells cell NNS 10_1101-2021_01_08_425864 186 12 . . . 10_1101-2021_01_08_425864 187 1 Means Means NNPS 10_1101-2021_01_08_425864 187 2 ± ± NNP 10_1101-2021_01_08_425864 187 3 SD sd NN 10_1101-2021_01_08_425864 187 4 of of IN 10_1101-2021_01_08_425864 187 5 three three CD 10_1101-2021_01_08_425864 187 6 independent independent JJ 10_1101-2021_01_08_425864 187 7 experiments experiment NNS 10_1101-2021_01_08_425864 187 8 . . . 10_1101-2021_01_08_425864 188 1 ICS ics NN 10_1101-2021_01_08_425864 188 2 is be VBZ 10_1101-2021_01_08_425864 188 3 internal internal JJ 10_1101-2021_01_08_425864 188 4 317 317 CD 10_1101-2021_01_08_425864 188 5 carbon carbon NN 10_1101-2021_01_08_425864 188 6 sources source NNS 10_1101-2021_01_08_425864 188 7 ; ; : 10_1101-2021_01_08_425864 188 8 nd nd NNP 10_1101-2021_01_08_425864 188 9 is be VBZ 10_1101-2021_01_08_425864 188 10 non non JJ 10_1101-2021_01_08_425864 188 11 - - JJ 10_1101-2021_01_08_425864 188 12 detectable detectable JJ 10_1101-2021_01_08_425864 188 13 . . . 10_1101-2021_01_08_425864 189 1 B b NN 10_1101-2021_01_08_425864 189 2 ) ) -RRB- 10_1101-2021_01_08_425864 189 3 and and CC 10_1101-2021_01_08_425864 189 4 C C NNP 10_1101-2021_01_08_425864 189 5 ) ) -RRB- 10_1101-2021_01_08_425864 189 6 Schematic schematic JJ 10_1101-2021_01_08_425864 189 7 representation representation NN 10_1101-2021_01_08_425864 189 8 of of IN 10_1101-2021_01_08_425864 189 9 the the DT 10_1101-2021_01_08_425864 189 10 contribution contribution NN 10_1101-2021_01_08_425864 189 11 of of IN 10_1101-2021_01_08_425864 189 12 318 318 CD 10_1101-2021_01_08_425864 189 13 glucose glucose NN 10_1101-2021_01_08_425864 189 14 and and CC 10_1101-2021_01_08_425864 189 15 palmitate palmitate VBP 10_1101-2021_01_08_425864 189 16 to to IN 10_1101-2021_01_08_425864 189 17 the the DT 10_1101-2021_01_08_425864 189 18 metabolism metabolism NN 10_1101-2021_01_08_425864 189 19 of of IN 10_1101-2021_01_08_425864 189 20 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 189 21 forms form NNS 10_1101-2021_01_08_425864 189 22 of of IN 10_1101-2021_01_08_425864 189 23 T. T. NNP 10_1101-2021_01_08_425864 189 24 cruzi cruzi NNS 10_1101-2021_01_08_425864 189 25 . . . 10_1101-2021_01_08_425864 190 1 The the DT 10_1101-2021_01_08_425864 190 2 glycosomal glycosomal NN 10_1101-2021_01_08_425864 190 3 319 319 CD 10_1101-2021_01_08_425864 190 4 compartment compartment NN 10_1101-2021_01_08_425864 190 5 and and CC 10_1101-2021_01_08_425864 190 6 TCA TCA NNP 10_1101-2021_01_08_425864 190 7 cycle cycle NN 10_1101-2021_01_08_425864 190 8 are be VBP 10_1101-2021_01_08_425864 190 9 indicated indicate VBN 10_1101-2021_01_08_425864 190 10 . . . 10_1101-2021_01_08_425864 191 1 The the DT 10_1101-2021_01_08_425864 191 2 amount amount NN 10_1101-2021_01_08_425864 191 3 of of IN 10_1101-2021_01_08_425864 191 4 end end NN 10_1101-2021_01_08_425864 191 5 - - HYPH 10_1101-2021_01_08_425864 191 6 product product NN 10_1101-2021_01_08_425864 191 7 determined determine VBN 10_1101-2021_01_08_425864 191 8 by by IN 10_1101-2021_01_08_425864 191 9 the the DT 10_1101-2021_01_08_425864 191 10 font font NN 10_1101-2021_01_08_425864 191 11 size size NN 10_1101-2021_01_08_425864 191 12 . . . 10_1101-2021_01_08_425864 192 1 320 320 CD 10_1101-2021_01_08_425864 192 2 Numbers number NNS 10_1101-2021_01_08_425864 192 3 indicates indicate VBZ 10_1101-2021_01_08_425864 192 4 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 192 5 steps step NNS 10_1101-2021_01_08_425864 192 6 . . . 10_1101-2021_01_08_425864 193 1 1 1 LS 10_1101-2021_01_08_425864 193 2 . . . 10_1101-2021_01_08_425864 194 1 Glycolysis glycolysis NN 10_1101-2021_01_08_425864 194 2 ; ; : 10_1101-2021_01_08_425864 194 3 2 2 LS 10_1101-2021_01_08_425864 194 4 . . . 10_1101-2021_01_08_425864 194 5 pyruvate pyruvate VB 10_1101-2021_01_08_425864 194 6 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 194 7 ; ; : 10_1101-2021_01_08_425864 194 8 3 3 CD 10_1101-2021_01_08_425864 194 9 . . . 10_1101-2021_01_08_425864 194 10 citrate citrate NNP 10_1101-2021_01_08_425864 194 11 synthase synthase NN 10_1101-2021_01_08_425864 194 12 ; ; : 10_1101-2021_01_08_425864 194 13 4 4 CD 10_1101-2021_01_08_425864 194 14 . . . 10_1101-2021_01_08_425864 195 1 321 321 CD 10_1101-2021_01_08_425864 195 2 aconitase aconitase NN 10_1101-2021_01_08_425864 195 3 ; ; : 10_1101-2021_01_08_425864 195 4 5 5 LS 10_1101-2021_01_08_425864 195 5 . . . 10_1101-2021_01_08_425864 195 6 isocitrate isocitrate VB 10_1101-2021_01_08_425864 195 7 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 195 8 ; ; : 10_1101-2021_01_08_425864 195 9 6 6 CD 10_1101-2021_01_08_425864 195 10 . . . 10_1101-2021_01_08_425864 195 11 α α LS 10_1101-2021_01_08_425864 195 12 - - HYPH 10_1101-2021_01_08_425864 195 13 ketoglutarate ketoglutarate NNP 10_1101-2021_01_08_425864 195 14 dehydrogenase dehydrogenase NNP 10_1101-2021_01_08_425864 195 15 ; ; : 10_1101-2021_01_08_425864 195 16 7 7 CD 10_1101-2021_01_08_425864 195 17 . . . 10_1101-2021_01_08_425864 195 18 succinyl succinyl NNP 10_1101-2021_01_08_425864 195 19 - - HYPH 10_1101-2021_01_08_425864 195 20 CoA CoA NNP 10_1101-2021_01_08_425864 195 21 322 322 CD 10_1101-2021_01_08_425864 195 22 synthetase synthetase NN 10_1101-2021_01_08_425864 195 23 ; ; : 10_1101-2021_01_08_425864 195 24 8 8 CD 10_1101-2021_01_08_425864 195 25 . . . 10_1101-2021_01_08_425864 196 1 Succinate succinate VB 10_1101-2021_01_08_425864 196 2 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 196 3 / / SYM 10_1101-2021_01_08_425864 196 4 complex complex JJ 10_1101-2021_01_08_425864 196 5 II ii CD 10_1101-2021_01_08_425864 196 6 / / SYM 10_1101-2021_01_08_425864 196 7 fumarate fumarate NN 10_1101-2021_01_08_425864 196 8 reductase reductase NN 10_1101-2021_01_08_425864 196 9 NADH NADH NNP 10_1101-2021_01_08_425864 196 10 - - HYPH 10_1101-2021_01_08_425864 196 11 dependent dependent JJ 10_1101-2021_01_08_425864 196 12 ; ; : 10_1101-2021_01_08_425864 196 13 9 9 CD 10_1101-2021_01_08_425864 196 14 . . . 10_1101-2021_01_08_425864 197 1 323 323 CD 10_1101-2021_01_08_425864 197 2 fumarate fumarate NN 10_1101-2021_01_08_425864 197 3 hydratase hydratase NN 10_1101-2021_01_08_425864 197 4 ; ; : 10_1101-2021_01_08_425864 197 5 10 10 CD 10_1101-2021_01_08_425864 197 6 . . . 10_1101-2021_01_08_425864 197 7 malate malate NNP 10_1101-2021_01_08_425864 197 8 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 197 9 ; ; : 10_1101-2021_01_08_425864 197 10 11 11 CD 10_1101-2021_01_08_425864 197 11 . . . 10_1101-2021_01_08_425864 198 1 Malic malic JJ 10_1101-2021_01_08_425864 198 2 enzyme enzyme NNS 10_1101-2021_01_08_425864 198 3 ; ; : 10_1101-2021_01_08_425864 198 4 12 12 CD 10_1101-2021_01_08_425864 198 5 . . . 10_1101-2021_01_08_425864 198 6 alanine alanine NNP 10_1101-2021_01_08_425864 198 7 dehydrogenase dehydrogenase NNP 10_1101-2021_01_08_425864 198 8 / / SYM 10_1101-2021_01_08_425864 198 9 alanine alanine NNP 10_1101-2021_01_08_425864 198 10 324 324 CD 10_1101-2021_01_08_425864 198 11 aminotransferase aminotransferase NN 10_1101-2021_01_08_425864 198 12 ; ; : 10_1101-2021_01_08_425864 198 13 13 13 CD 10_1101-2021_01_08_425864 198 14 . . . 10_1101-2021_01_08_425864 198 15 lactate lactate JJ 10_1101-2021_01_08_425864 198 16 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 198 17 ; ; : 10_1101-2021_01_08_425864 198 18 14 14 CD 10_1101-2021_01_08_425864 198 19 . . . 10_1101-2021_01_08_425864 198 20 acetate acetate NNP 10_1101-2021_01_08_425864 198 21 : : : 10_1101-2021_01_08_425864 198 22 succinyl succinyl NNP 10_1101-2021_01_08_425864 198 23 - - HYPH 10_1101-2021_01_08_425864 198 24 CoA CoA NNP 10_1101-2021_01_08_425864 198 25 transferase transferase NN 10_1101-2021_01_08_425864 198 26 ; ; : 10_1101-2021_01_08_425864 198 27 15 15 CD 10_1101-2021_01_08_425864 198 28 . . . 10_1101-2021_01_08_425864 198 29 acetyl acetyl NNP 10_1101-2021_01_08_425864 198 30 - - HYPH 10_1101-2021_01_08_425864 198 31 CoA CoA NNP 10_1101-2021_01_08_425864 198 32 325 325 CD 10_1101-2021_01_08_425864 198 33 hydrolase hydrolase NN 10_1101-2021_01_08_425864 198 34 ; ; : 10_1101-2021_01_08_425864 198 35 16 16 CD 10_1101-2021_01_08_425864 198 36 . . . 10_1101-2021_01_08_425864 198 37 succinyl succinyl NNP 10_1101-2021_01_08_425864 198 38 - - HYPH 10_1101-2021_01_08_425864 198 39 CoA CoA NNP 10_1101-2021_01_08_425864 198 40 synthetase synthetase NN 10_1101-2021_01_08_425864 198 41 ; ; : 10_1101-2021_01_08_425864 198 42 17 17 CD 10_1101-2021_01_08_425864 198 43 . . . 10_1101-2021_01_08_425864 199 1 Glycosomal glycosomal JJ 10_1101-2021_01_08_425864 199 2 fumarate fumarate NN 10_1101-2021_01_08_425864 199 3 reductase reductase NN 10_1101-2021_01_08_425864 199 4 and and CC 10_1101-2021_01_08_425864 199 5 18 18 CD 10_1101-2021_01_08_425864 199 6 . . . 10_1101-2021_01_08_425864 200 1 Palmitate palmitate NN 10_1101-2021_01_08_425864 200 2 326 326 CD 10_1101-2021_01_08_425864 200 3 oxidation oxidation NN 10_1101-2021_01_08_425864 200 4 by by IN 10_1101-2021_01_08_425864 200 5 beta beta NN 10_1101-2021_01_08_425864 200 6 - - HYPH 10_1101-2021_01_08_425864 200 7 oxidation oxidation NN 10_1101-2021_01_08_425864 200 8 , , , 10_1101-2021_01_08_425864 200 9 resulting result VBG 10_1101-2021_01_08_425864 200 10 in in IN 10_1101-2021_01_08_425864 200 11 FADH2 FADH2 NNP 10_1101-2021_01_08_425864 200 12 , , , 10_1101-2021_01_08_425864 200 13 NADH NADH NNP 10_1101-2021_01_08_425864 200 14 and and CC 10_1101-2021_01_08_425864 200 15 acetyl acetyl NNP 10_1101-2021_01_08_425864 200 16 - - HYPH 10_1101-2021_01_08_425864 200 17 CoA CoA NNP 10_1101-2021_01_08_425864 200 18 ; ; : 10_1101-2021_01_08_425864 200 19 Abbreviations Abbreviations NNP 10_1101-2021_01_08_425864 200 20 : : : 10_1101-2021_01_08_425864 200 21 Cit Cit NNP 10_1101-2021_01_08_425864 200 22 : : : 10_1101-2021_01_08_425864 200 23 Citrate Citrate NNP 10_1101-2021_01_08_425864 200 24 , , , 10_1101-2021_01_08_425864 200 25 327 327 CD 10_1101-2021_01_08_425864 200 26 Aco aco JJ 10_1101-2021_01_08_425864 200 27 : : : 10_1101-2021_01_08_425864 200 28 Aconitate Aconitate NNP 10_1101-2021_01_08_425864 200 29 , , , 10_1101-2021_01_08_425864 200 30 IsoC IsoC NNP 10_1101-2021_01_08_425864 200 31 : : : 10_1101-2021_01_08_425864 200 32 Isocitrate isocitrate VB 10_1101-2021_01_08_425864 200 33 , , , 10_1101-2021_01_08_425864 200 34 α α NNP 10_1101-2021_01_08_425864 200 35 - - HYPH 10_1101-2021_01_08_425864 200 36 kg kg NNS 10_1101-2021_01_08_425864 200 37 : : : 10_1101-2021_01_08_425864 200 38 α α LS 10_1101-2021_01_08_425864 200 39 - - : 10_1101-2021_01_08_425864 200 40 Ketoglutarate Ketoglutarate NNP 10_1101-2021_01_08_425864 200 41 , , , 10_1101-2021_01_08_425864 200 42 Suc Suc NNP 10_1101-2021_01_08_425864 200 43 - - HYPH 10_1101-2021_01_08_425864 200 44 CoA CoA NNP 10_1101-2021_01_08_425864 200 45 : : : 10_1101-2021_01_08_425864 200 46 Succinyl Succinyl NNP 10_1101-2021_01_08_425864 200 47 - - HYPH 10_1101-2021_01_08_425864 200 48 CoA CoA NNP 10_1101-2021_01_08_425864 200 49 , , , 10_1101-2021_01_08_425864 200 50 Suc Suc NNP 10_1101-2021_01_08_425864 200 51 : : : 10_1101-2021_01_08_425864 200 52 Succinate Succinate NNP 10_1101-2021_01_08_425864 200 53 , , , 10_1101-2021_01_08_425864 200 54 328 328 CD 10_1101-2021_01_08_425864 200 55 Fum Fum NNP 10_1101-2021_01_08_425864 200 56 : : : 10_1101-2021_01_08_425864 200 57 Fumarate Fumarate NNP 10_1101-2021_01_08_425864 200 58 , , , 10_1101-2021_01_08_425864 200 59 Mal Mal NNP 10_1101-2021_01_08_425864 200 60 : : : 10_1101-2021_01_08_425864 200 61 Malate Malate NNP 10_1101-2021_01_08_425864 200 62 , , , 10_1101-2021_01_08_425864 200 63 and and CC 10_1101-2021_01_08_425864 200 64 Oxa oxa NN 10_1101-2021_01_08_425864 200 65 : : : 10_1101-2021_01_08_425864 200 66 Oxaloacetate oxaloacetate VB 10_1101-2021_01_08_425864 200 67 . . . 10_1101-2021_01_08_425864 201 1 329 329 CD 10_1101-2021_01_08_425864 201 2 330 330 CD 10_1101-2021_01_08_425864 201 3 Glucose Glucose NNP 10_1101-2021_01_08_425864 201 4 metabolism metabolism NN 10_1101-2021_01_08_425864 201 5 represses repress VBZ 10_1101-2021_01_08_425864 201 6 the the DT 10_1101-2021_01_08_425864 201 7 fatty fatty NN 10_1101-2021_01_08_425864 201 8 acid acid NN 10_1101-2021_01_08_425864 201 9 oxidation oxidation NN 10_1101-2021_01_08_425864 201 10 in in IN 10_1101-2021_01_08_425864 201 11 epimastigotes epimastigote VBZ 10_1101-2021_01_08_425864 201 12 331 331 CD 10_1101-2021_01_08_425864 201 13 Glucose Glucose NNP 10_1101-2021_01_08_425864 201 14 is be VBZ 10_1101-2021_01_08_425864 201 15 the the DT 10_1101-2021_01_08_425864 201 16 primary primary JJ 10_1101-2021_01_08_425864 201 17 carbon carbon NN 10_1101-2021_01_08_425864 201 18 source source NN 10_1101-2021_01_08_425864 201 19 for for IN 10_1101-2021_01_08_425864 201 20 exponentially exponentially RB 10_1101-2021_01_08_425864 201 21 proliferating proliferate VBG 10_1101-2021_01_08_425864 201 22 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 201 23 , , , 10_1101-2021_01_08_425864 201 24 and and CC 10_1101-2021_01_08_425864 201 25 after after IN 10_1101-2021_01_08_425864 201 26 332 332 CD 10_1101-2021_01_08_425864 201 27 its -PRON- PRP$ 10_1101-2021_01_08_425864 201 28 exhaustion exhaustion NN 10_1101-2021_01_08_425864 201 29 from from IN 10_1101-2021_01_08_425864 201 30 the the DT 10_1101-2021_01_08_425864 201 31 culture culture NN 10_1101-2021_01_08_425864 201 32 medium medium NN 10_1101-2021_01_08_425864 201 33 , , , 10_1101-2021_01_08_425864 201 34 the the DT 10_1101-2021_01_08_425864 201 35 parasites parasite NNS 10_1101-2021_01_08_425864 201 36 change change VBP 10_1101-2021_01_08_425864 201 37 their -PRON- PRP$ 10_1101-2021_01_08_425864 201 38 metabolism metabolism NN 10_1101-2021_01_08_425864 201 39 to to TO 10_1101-2021_01_08_425864 201 40 use use VB 10_1101-2021_01_08_425864 201 41 amino amino JJ 10_1101-2021_01_08_425864 201 42 acids acid NNS 10_1101-2021_01_08_425864 201 43 as as IN 10_1101-2021_01_08_425864 201 44 333 333 CD 10_1101-2021_01_08_425864 201 45 carbon carbon NN 10_1101-2021_01_08_425864 201 46 sources source NNS 10_1101-2021_01_08_425864 201 47 preferentially preferentially RB 10_1101-2021_01_08_425864 201 48 [ [ -LRB- 10_1101-2021_01_08_425864 201 49 10 10 CD 10_1101-2021_01_08_425864 201 50 ] ] -RRB- 10_1101-2021_01_08_425864 201 51 . . . 10_1101-2021_01_08_425864 202 1 Therefore therefore RB 10_1101-2021_01_08_425864 202 2 , , , 10_1101-2021_01_08_425864 202 3 we -PRON- PRP 10_1101-2021_01_08_425864 202 4 were be VBD 10_1101-2021_01_08_425864 202 5 interested interested JJ 10_1101-2021_01_08_425864 202 6 in in IN 10_1101-2021_01_08_425864 202 7 analysing analyse VBG 10_1101-2021_01_08_425864 202 8 if if IN 10_1101-2021_01_08_425864 202 9 this this DT 10_1101-2021_01_08_425864 202 10 preference preference NN 10_1101-2021_01_08_425864 202 11 for for IN 10_1101-2021_01_08_425864 202 12 334 334 CD 10_1101-2021_01_08_425864 202 13 glucose glucose NN 10_1101-2021_01_08_425864 202 14 is be VBZ 10_1101-2021_01_08_425864 202 15 maintained maintain VBN 10_1101-2021_01_08_425864 202 16 in in IN 10_1101-2021_01_08_425864 202 17 relation relation NN 10_1101-2021_01_08_425864 202 18 to to IN 10_1101-2021_01_08_425864 202 19 the the DT 10_1101-2021_01_08_425864 202 20 consumption consumption NN 10_1101-2021_01_08_425864 202 21 of of IN 10_1101-2021_01_08_425864 202 22 lipids lipid NNS 10_1101-2021_01_08_425864 202 23 . . . 10_1101-2021_01_08_425864 203 1 To to TO 10_1101-2021_01_08_425864 203 2 determine determine VB 10_1101-2021_01_08_425864 203 3 if if IN 10_1101-2021_01_08_425864 203 4 glucose glucose NN 10_1101-2021_01_08_425864 203 5 metabolism metabolism VBG 10_1101-2021_01_08_425864 203 6 335 335 CD 10_1101-2021_01_08_425864 203 7 interferes interfere NNS 10_1101-2021_01_08_425864 203 8 with with IN 10_1101-2021_01_08_425864 203 9 the the DT 10_1101-2021_01_08_425864 203 10 consumption consumption NN 10_1101-2021_01_08_425864 203 11 of of IN 10_1101-2021_01_08_425864 203 12 fatty fatty NN 10_1101-2021_01_08_425864 203 13 acids acid NNS 10_1101-2021_01_08_425864 203 14 , , , 10_1101-2021_01_08_425864 203 15 we -PRON- PRP 10_1101-2021_01_08_425864 203 16 created create VBD 10_1101-2021_01_08_425864 203 17 a a DT 10_1101-2021_01_08_425864 203 18 48 48 CD 10_1101-2021_01_08_425864 203 19 h h NN 10_1101-2021_01_08_425864 203 20 proliferation proliferation NN 10_1101-2021_01_08_425864 203 21 curve curve NN 10_1101-2021_01_08_425864 203 22 using use VBG 10_1101-2021_01_08_425864 203 23 parasites parasite NNS 10_1101-2021_01_08_425864 203 24 336 336 CD 10_1101-2021_01_08_425864 203 25 with with IN 10_1101-2021_01_08_425864 203 26 an an DT 10_1101-2021_01_08_425864 203 27 initial initial JJ 10_1101-2021_01_08_425864 203 28 concentration concentration NN 10_1101-2021_01_08_425864 203 29 adjusted adjust VBN 10_1101-2021_01_08_425864 203 30 to to IN 10_1101-2021_01_08_425864 203 31 2.5 2.5 CD 10_1101-2021_01_08_425864 203 32 x x SYM 10_1101-2021_01_08_425864 203 33 107 107 CD 10_1101-2021_01_08_425864 203 34 mL-1 ml-1 NN 10_1101-2021_01_08_425864 203 35 and and CC 10_1101-2021_01_08_425864 203 36 quantified quantify VBD 10_1101-2021_01_08_425864 203 37 them -PRON- PRP 10_1101-2021_01_08_425864 203 38 for for IN 10_1101-2021_01_08_425864 203 39 24 24 CD 10_1101-2021_01_08_425864 203 40 h h NNS 10_1101-2021_01_08_425864 203 41 each each DT 10_1101-2021_01_08_425864 203 42 . . . 10_1101-2021_01_08_425864 204 1 Under under IN 10_1101-2021_01_08_425864 204 2 these these DT 10_1101-2021_01_08_425864 204 3 337 337 CD 10_1101-2021_01_08_425864 204 4 conditions condition NNS 10_1101-2021_01_08_425864 204 5 , , , 10_1101-2021_01_08_425864 204 6 the the DT 10_1101-2021_01_08_425864 204 7 parasites parasite NNS 10_1101-2021_01_08_425864 204 8 from from IN 10_1101-2021_01_08_425864 204 9 the the DT 10_1101-2021_01_08_425864 204 10 beginning beginning NN 10_1101-2021_01_08_425864 204 11 of of IN 10_1101-2021_01_08_425864 204 12 the the DT 10_1101-2021_01_08_425864 204 13 experiment experiment NN 10_1101-2021_01_08_425864 204 14 , , , 10_1101-2021_01_08_425864 204 15 at at IN 10_1101-2021_01_08_425864 204 16 0 0 CD 10_1101-2021_01_08_425864 204 17 h h NN 10_1101-2021_01_08_425864 204 18 , , , 10_1101-2021_01_08_425864 204 19 are be VBP 10_1101-2021_01_08_425864 204 20 at at IN 10_1101-2021_01_08_425864 204 21 mid mid JJ 10_1101-2021_01_08_425864 204 22 - - JJ 10_1101-2021_01_08_425864 204 23 exponential exponential JJ 10_1101-2021_01_08_425864 204 24 phase phase NN 10_1101-2021_01_08_425864 204 25 , , , 10_1101-2021_01_08_425864 204 26 338 338 CD 10_1101-2021_01_08_425864 204 27 they -PRON- PRP 10_1101-2021_01_08_425864 204 28 are be VBP 10_1101-2021_01_08_425864 204 29 at at IN 10_1101-2021_01_08_425864 204 30 late late JJ 10_1101-2021_01_08_425864 204 31 exponential exponential JJ 10_1101-2021_01_08_425864 204 32 phase phase NN 10_1101-2021_01_08_425864 204 33 at at IN 10_1101-2021_01_08_425864 204 34 24 24 CD 10_1101-2021_01_08_425864 204 35 h h NN 10_1101-2021_01_08_425864 204 36 , , , 10_1101-2021_01_08_425864 204 37 and and CC 10_1101-2021_01_08_425864 204 38 at at IN 10_1101-2021_01_08_425864 204 39 48 48 CD 10_1101-2021_01_08_425864 204 40 h h NN 10_1101-2021_01_08_425864 204 41 they -PRON- PRP 10_1101-2021_01_08_425864 204 42 reached reach VBD 10_1101-2021_01_08_425864 204 43 stationary stationary JJ 10_1101-2021_01_08_425864 204 44 phase phase NN 10_1101-2021_01_08_425864 204 45 at at IN 10_1101-2021_01_08_425864 204 46 a a DT 10_1101-2021_01_08_425864 204 47 concentration concentration NN 10_1101-2021_01_08_425864 204 48 339 339 CD 10_1101-2021_01_08_425864 204 49 of of IN 10_1101-2021_01_08_425864 204 50 10 10 CD 10_1101-2021_01_08_425864 204 51 x x SYM 10_1101-2021_01_08_425864 204 52 107 107 CD 10_1101-2021_01_08_425864 204 53 mL-1 ml-1 NN 10_1101-2021_01_08_425864 204 54 ( ( -LRB- 10_1101-2021_01_08_425864 204 55 Fig Fig NNP 10_1101-2021_01_08_425864 204 56 3A 3A NNP 10_1101-2021_01_08_425864 204 57 ) ) -RRB- 10_1101-2021_01_08_425864 204 58 . . . 10_1101-2021_01_08_425864 205 1 At at IN 10_1101-2021_01_08_425864 205 2 0 0 CD 10_1101-2021_01_08_425864 205 3 h h NN 10_1101-2021_01_08_425864 205 4 , , , 10_1101-2021_01_08_425864 205 5 24 24 CD 10_1101-2021_01_08_425864 205 6 h h NN 10_1101-2021_01_08_425864 205 7 and and CC 10_1101-2021_01_08_425864 205 8 48 48 CD 10_1101-2021_01_08_425864 205 9 h h NNS 10_1101-2021_01_08_425864 205 10 , , , 10_1101-2021_01_08_425864 205 11 the the DT 10_1101-2021_01_08_425864 205 12 culture culture NN 10_1101-2021_01_08_425864 205 13 medium medium NN 10_1101-2021_01_08_425864 205 14 was be VBD 10_1101-2021_01_08_425864 205 15 collected collect VBN 10_1101-2021_01_08_425864 205 16 to to TO 10_1101-2021_01_08_425864 205 17 measure measure VB 10_1101-2021_01_08_425864 205 18 the the DT 10_1101-2021_01_08_425864 205 19 340 340 CD 10_1101-2021_01_08_425864 205 20 remaining remain VBG 10_1101-2021_01_08_425864 205 21 glucose glucose NN 10_1101-2021_01_08_425864 205 22 and and CC 10_1101-2021_01_08_425864 205 23 triacylglycerol triacylglycerol NN 10_1101-2021_01_08_425864 205 24 ( ( -LRB- 10_1101-2021_01_08_425864 205 25 TAGs TAGs NNP 10_1101-2021_01_08_425864 205 26 ) ) -RRB- 10_1101-2021_01_08_425864 205 27 concentrations concentration NNS 10_1101-2021_01_08_425864 205 28 ( ( -LRB- 10_1101-2021_01_08_425864 205 29 Figs fig NNS 10_1101-2021_01_08_425864 205 30 3B 3b NN 10_1101-2021_01_08_425864 205 31 and and CC 10_1101-2021_01_08_425864 205 32 3C 3c NN 10_1101-2021_01_08_425864 205 33 ) ) -RRB- 10_1101-2021_01_08_425864 205 34 . . . 10_1101-2021_01_08_425864 206 1 Most Most JJS 10_1101-2021_01_08_425864 206 2 of of IN 10_1101-2021_01_08_425864 206 3 the the DT 10_1101-2021_01_08_425864 206 4 glucose glucose NN 10_1101-2021_01_08_425864 206 5 341 341 CD 10_1101-2021_01_08_425864 206 6 was be VBD 10_1101-2021_01_08_425864 206 7 consumed consume VBN 10_1101-2021_01_08_425864 206 8 during during IN 10_1101-2021_01_08_425864 206 9 the the DT 10_1101-2021_01_08_425864 206 10 first first JJ 10_1101-2021_01_08_425864 206 11 24 24 CD 10_1101-2021_01_08_425864 206 12 h h NN 10_1101-2021_01_08_425864 206 13 ( ( -LRB- 10_1101-2021_01_08_425864 206 14 during during IN 10_1101-2021_01_08_425864 206 15 proliferation proliferation NN 10_1101-2021_01_08_425864 206 16 ) ) -RRB- 10_1101-2021_01_08_425864 206 17 , , , 10_1101-2021_01_08_425864 206 18 while while IN 10_1101-2021_01_08_425864 206 19 the the DT 10_1101-2021_01_08_425864 206 20 concentration concentration NN 10_1101-2021_01_08_425864 206 21 of of IN 10_1101-2021_01_08_425864 206 22 TAGs tag NNS 10_1101-2021_01_08_425864 206 23 remained remain VBD 10_1101-2021_01_08_425864 206 24 342 342 CD 10_1101-2021_01_08_425864 206 25 the the DT 10_1101-2021_01_08_425864 206 26 same same JJ 10_1101-2021_01_08_425864 206 27 . . . 10_1101-2021_01_08_425864 207 1 After after IN 10_1101-2021_01_08_425864 207 2 48 48 CD 10_1101-2021_01_08_425864 207 3 h h NN 10_1101-2021_01_08_425864 207 4 of of IN 10_1101-2021_01_08_425864 207 5 proliferation proliferation NN 10_1101-2021_01_08_425864 207 6 ( ( -LRB- 10_1101-2021_01_08_425864 207 7 stationary stationary JJ 10_1101-2021_01_08_425864 207 8 phase phase NN 10_1101-2021_01_08_425864 207 9 ) ) -RRB- 10_1101-2021_01_08_425864 207 10 , , , 10_1101-2021_01_08_425864 207 11 the the DT 10_1101-2021_01_08_425864 207 12 TAG TAG NNP 10_1101-2021_01_08_425864 207 13 levels level NNS 10_1101-2021_01_08_425864 207 14 and and CC 10_1101-2021_01_08_425864 207 15 lipid lipid NN 10_1101-2021_01_08_425864 207 16 contents content NNS 10_1101-2021_01_08_425864 207 17 of of IN 10_1101-2021_01_08_425864 207 18 the the DT 10_1101-2021_01_08_425864 207 19 343 343 CD 10_1101-2021_01_08_425864 207 20 droplets droplet NNS 10_1101-2021_01_08_425864 207 21 were be VBD 10_1101-2021_01_08_425864 207 22 decreased decrease VBN 10_1101-2021_01_08_425864 207 23 by by IN 10_1101-2021_01_08_425864 207 24 1.5-fold 1.5-fold CD 10_1101-2021_01_08_425864 207 25 and and CC 10_1101-2021_01_08_425864 207 26 2-fold 2-fold CD 10_1101-2021_01_08_425864 207 27 , , , 10_1101-2021_01_08_425864 207 28 respectively respectively RB 10_1101-2021_01_08_425864 207 29 , , , 10_1101-2021_01_08_425864 207 30 suggesting suggest VBG 10_1101-2021_01_08_425864 207 31 that that IN 10_1101-2021_01_08_425864 207 32 glucose glucose NN 10_1101-2021_01_08_425864 207 33 is be VBZ 10_1101-2021_01_08_425864 207 34 preferentially preferentially RB 10_1101-2021_01_08_425864 207 35 344 344 CD 10_1101-2021_01_08_425864 207 36 consumed consume VBN 10_1101-2021_01_08_425864 207 37 relative relative JJ 10_1101-2021_01_08_425864 207 38 to to IN 10_1101-2021_01_08_425864 207 39 fatty fatty NN 10_1101-2021_01_08_425864 207 40 acids acid NNS 10_1101-2021_01_08_425864 207 41 . . . 10_1101-2021_01_08_425864 208 1 These these DT 10_1101-2021_01_08_425864 208 2 data datum NNS 10_1101-2021_01_08_425864 208 3 show show VBP 10_1101-2021_01_08_425864 208 4 a a DT 10_1101-2021_01_08_425864 208 5 decrease decrease NN 10_1101-2021_01_08_425864 208 6 in in IN 10_1101-2021_01_08_425864 208 7 the the DT 10_1101-2021_01_08_425864 208 8 extracellular extracellular JJ 10_1101-2021_01_08_425864 208 9 TAGs tag NNS 10_1101-2021_01_08_425864 208 10 between between IN 10_1101-2021_01_08_425864 208 11 24 24 CD 10_1101-2021_01_08_425864 208 12 345 345 CD 10_1101-2021_01_08_425864 208 13 and and CC 10_1101-2021_01_08_425864 208 14 48 48 CD 10_1101-2021_01_08_425864 208 15 h h NNS 10_1101-2021_01_08_425864 208 16 , , , 10_1101-2021_01_08_425864 208 17 while while IN 10_1101-2021_01_08_425864 208 18 the the DT 10_1101-2021_01_08_425864 208 19 glucose glucose NN 10_1101-2021_01_08_425864 208 20 was be VBD 10_1101-2021_01_08_425864 208 21 already already RB 10_1101-2021_01_08_425864 208 22 almost almost RB 10_1101-2021_01_08_425864 208 23 entirely entirely RB 10_1101-2021_01_08_425864 208 24 consumed consume VBN 10_1101-2021_01_08_425864 208 25 , , , 10_1101-2021_01_08_425864 208 26 suggesting suggest VBG 10_1101-2021_01_08_425864 208 27 that that IN 10_1101-2021_01_08_425864 208 28 glucose glucose NN 10_1101-2021_01_08_425864 208 29 is be VBZ 10_1101-2021_01_08_425864 208 30 346 346 CD 10_1101-2021_01_08_425864 208 31 negatively negatively RB 10_1101-2021_01_08_425864 208 32 regulating regulate VBG 10_1101-2021_01_08_425864 208 33 the the DT 10_1101-2021_01_08_425864 208 34 fatty fatty NN 10_1101-2021_01_08_425864 208 35 acid acid NN 10_1101-2021_01_08_425864 208 36 catabolism catabolism NN 10_1101-2021_01_08_425864 208 37 . . . 10_1101-2021_01_08_425864 209 1 347 347 CD 10_1101-2021_01_08_425864 209 2 348 348 CD 10_1101-2021_01_08_425864 209 3 .CC .cc CD 10_1101-2021_01_08_425864 209 4 - - : 10_1101-2021_01_08_425864 209 5 BY by IN 10_1101-2021_01_08_425864 209 6 4.0 4.0 CD 10_1101-2021_01_08_425864 209 7 International international JJ 10_1101-2021_01_08_425864 209 8 licensemade licensemade NN 10_1101-2021_01_08_425864 209 9 available available JJ 10_1101-2021_01_08_425864 209 10 under under IN 10_1101-2021_01_08_425864 209 11 a a DT 10_1101-2021_01_08_425864 209 12 ( ( -LRB- 10_1101-2021_01_08_425864 209 13 which which WDT 10_1101-2021_01_08_425864 209 14 was be VBD 10_1101-2021_01_08_425864 209 15 not not RB 10_1101-2021_01_08_425864 209 16 certified certify VBN 10_1101-2021_01_08_425864 209 17 by by IN 10_1101-2021_01_08_425864 209 18 peer peer NN 10_1101-2021_01_08_425864 209 19 review review NN 10_1101-2021_01_08_425864 209 20 ) ) -RRB- 10_1101-2021_01_08_425864 209 21 is be VBZ 10_1101-2021_01_08_425864 209 22 the the DT 10_1101-2021_01_08_425864 209 23 author author NN 10_1101-2021_01_08_425864 209 24 / / SYM 10_1101-2021_01_08_425864 209 25 funder funder NN 10_1101-2021_01_08_425864 209 26 , , , 10_1101-2021_01_08_425864 209 27 who who WP 10_1101-2021_01_08_425864 209 28 has have VBZ 10_1101-2021_01_08_425864 209 29 granted grant VBN 10_1101-2021_01_08_425864 209 30 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 209 31 a a DT 10_1101-2021_01_08_425864 209 32 license license NN 10_1101-2021_01_08_425864 209 33 to to TO 10_1101-2021_01_08_425864 209 34 display display VB 10_1101-2021_01_08_425864 209 35 the the DT 10_1101-2021_01_08_425864 209 36 preprint preprint NN 10_1101-2021_01_08_425864 209 37 in in IN 10_1101-2021_01_08_425864 209 38 perpetuity perpetuity NN 10_1101-2021_01_08_425864 209 39 . . . 10_1101-2021_01_08_425864 210 1 It -PRON- PRP 10_1101-2021_01_08_425864 210 2 is be VBZ 10_1101-2021_01_08_425864 210 3 The the DT 10_1101-2021_01_08_425864 210 4 copyright copyright NN 10_1101-2021_01_08_425864 210 5 holder holder NN 10_1101-2021_01_08_425864 210 6 for for IN 10_1101-2021_01_08_425864 210 7 this this DT 10_1101-2021_01_08_425864 210 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 210 9 version version NN 10_1101-2021_01_08_425864 210 10 posted post VBD 10_1101-2021_01_08_425864 210 11 January January NNP 10_1101-2021_01_08_425864 210 12 8 8 CD 10_1101-2021_01_08_425864 210 13 , , , 10_1101-2021_01_08_425864 210 14 2021 2021 CD 10_1101-2021_01_08_425864 210 15 . . . 10_1101-2021_01_08_425864 210 16 ; ; : 10_1101-2021_01_08_425864 210 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 210 18 : : : 10_1101-2021_01_08_425864 210 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 210 20 preprint preprint NN 10_1101-2021_01_08_425864 210 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 210 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 210 23 349 349 CD 10_1101-2021_01_08_425864 210 24 Figure figure NN 10_1101-2021_01_08_425864 210 25 3 3 CD 10_1101-2021_01_08_425864 210 26 . . . 10_1101-2021_01_08_425864 211 1 Changes change NNS 10_1101-2021_01_08_425864 211 2 in in IN 10_1101-2021_01_08_425864 211 3 glucose glucose NN 10_1101-2021_01_08_425864 211 4 and and CC 10_1101-2021_01_08_425864 211 5 triacylglycerol triacylglycerol NN 10_1101-2021_01_08_425864 211 6 contents content NNS 10_1101-2021_01_08_425864 211 7 in in IN 10_1101-2021_01_08_425864 211 8 LIT LIT NNP 10_1101-2021_01_08_425864 211 9 medium medium NN 10_1101-2021_01_08_425864 211 10 . . . 10_1101-2021_01_08_425864 212 1 A a LS 10_1101-2021_01_08_425864 212 2 ) ) -RRB- 10_1101-2021_01_08_425864 212 3 Growth growth NN 10_1101-2021_01_08_425864 212 4 curve curve NN 10_1101-2021_01_08_425864 212 5 of of IN 10_1101-2021_01_08_425864 212 6 350 350 CD 10_1101-2021_01_08_425864 212 7 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 212 8 forms form NNS 10_1101-2021_01_08_425864 212 9 . . . 10_1101-2021_01_08_425864 213 1 B b NN 10_1101-2021_01_08_425864 213 2 ) ) -RRB- 10_1101-2021_01_08_425864 213 3 Glucose Glucose NNP 10_1101-2021_01_08_425864 213 4 quantification quantification NN 10_1101-2021_01_08_425864 213 5 over over IN 10_1101-2021_01_08_425864 213 6 48 48 CD 10_1101-2021_01_08_425864 213 7 h. h. NN 10_1101-2021_01_08_425864 213 8 C C NNP 10_1101-2021_01_08_425864 213 9 ) ) -RRB- 10_1101-2021_01_08_425864 213 10 Triacylglycerol triacylglycerol NN 10_1101-2021_01_08_425864 213 11 levels level NNS 10_1101-2021_01_08_425864 213 12 over over IN 10_1101-2021_01_08_425864 213 13 48 48 CD 10_1101-2021_01_08_425864 213 14 h. h. NNS 10_1101-2021_01_08_425864 213 15 In in IN 10_1101-2021_01_08_425864 213 16 each each DT 10_1101-2021_01_08_425864 213 17 351 351 CD 10_1101-2021_01_08_425864 213 18 experiment experiment NN 10_1101-2021_01_08_425864 213 19 , , , 10_1101-2021_01_08_425864 213 20 we -PRON- PRP 10_1101-2021_01_08_425864 213 21 collected collect VBD 10_1101-2021_01_08_425864 213 22 each each DT 10_1101-2021_01_08_425864 213 23 medium medium NN 10_1101-2021_01_08_425864 213 24 at at IN 10_1101-2021_01_08_425864 213 25 different different JJ 10_1101-2021_01_08_425864 213 26 times time NNS 10_1101-2021_01_08_425864 213 27 and and CC 10_1101-2021_01_08_425864 213 28 subjected subject VBD 10_1101-2021_01_08_425864 213 29 it -PRON- PRP 10_1101-2021_01_08_425864 213 30 to to IN 10_1101-2021_01_08_425864 213 31 quantification quantification NN 10_1101-2021_01_08_425864 213 32 according accord VBG 10_1101-2021_01_08_425864 213 33 352 352 CD 10_1101-2021_01_08_425864 213 34 to to IN 10_1101-2021_01_08_425864 213 35 the the DT 10_1101-2021_01_08_425864 213 36 manufacturer manufacturer NN 10_1101-2021_01_08_425864 213 37 's 's POS 10_1101-2021_01_08_425864 213 38 instructions instruction NNS 10_1101-2021_01_08_425864 213 39 . . . 10_1101-2021_01_08_425864 214 1 All all PDT 10_1101-2021_01_08_425864 214 2 the the DT 10_1101-2021_01_08_425864 214 3 experiments experiment NNS 10_1101-2021_01_08_425864 214 4 were be VBD 10_1101-2021_01_08_425864 214 5 performed perform VBN 10_1101-2021_01_08_425864 214 6 in in IN 10_1101-2021_01_08_425864 214 7 triplicates triplicate NNS 10_1101-2021_01_08_425864 214 8 . . . 10_1101-2021_01_08_425864 215 1 Statistical statistical JJ 10_1101-2021_01_08_425864 215 2 353 353 CD 10_1101-2021_01_08_425864 215 3 analysis analysis NN 10_1101-2021_01_08_425864 215 4 was be VBD 10_1101-2021_01_08_425864 215 5 performed perform VBN 10_1101-2021_01_08_425864 215 6 with with IN 10_1101-2021_01_08_425864 215 7 one one CD 10_1101-2021_01_08_425864 215 8 - - HYPH 10_1101-2021_01_08_425864 215 9 way way NN 10_1101-2021_01_08_425864 215 10 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 215 11 followed follow VBN 10_1101-2021_01_08_425864 215 12 by by IN 10_1101-2021_01_08_425864 215 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 215 14 's 's POS 10_1101-2021_01_08_425864 215 15 post post JJ 10_1101-2021_01_08_425864 215 16 - - JJ 10_1101-2021_01_08_425864 215 17 test test JJ 10_1101-2021_01_08_425864 215 18 p p NN 10_1101-2021_01_08_425864 215 19 < < XX 10_1101-2021_01_08_425864 215 20 0.05 0.05 XX 10_1101-2021_01_08_425864 215 21 using use VBG 10_1101-2021_01_08_425864 215 22 the the DT 10_1101-2021_01_08_425864 215 23 354 354 CD 10_1101-2021_01_08_425864 215 24 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 215 25 Prism Prism NNP 10_1101-2021_01_08_425864 215 26 8.0.2 8.0.2 NNP 10_1101-2021_01_08_425864 215 27 software software NN 10_1101-2021_01_08_425864 215 28 program program NN 10_1101-2021_01_08_425864 215 29 . . . 10_1101-2021_01_08_425864 216 1 We -PRON- PRP 10_1101-2021_01_08_425864 216 2 represent represent VBP 10_1101-2021_01_08_425864 216 3 the the DT 10_1101-2021_01_08_425864 216 4 levels level NNS 10_1101-2021_01_08_425864 216 5 of of IN 10_1101-2021_01_08_425864 216 6 statistical statistical JJ 10_1101-2021_01_08_425864 216 7 significance significance NN 10_1101-2021_01_08_425864 216 8 in in IN 10_1101-2021_01_08_425864 216 9 this this DT 10_1101-2021_01_08_425864 216 10 355 355 CD 10_1101-2021_01_08_425864 216 11 figure figure NN 10_1101-2021_01_08_425864 216 12 as as IN 10_1101-2021_01_08_425864 216 13 follows follow VBZ 10_1101-2021_01_08_425864 216 14 : : : 10_1101-2021_01_08_425864 216 15 * * NFP 10_1101-2021_01_08_425864 216 16 * * NFP 10_1101-2021_01_08_425864 216 17 * * NFP 10_1101-2021_01_08_425864 216 18 p p NN 10_1101-2021_01_08_425864 216 19 value value NN 10_1101-2021_01_08_425864 216 20 < < XX 10_1101-2021_01_08_425864 216 21 0.001 0.001 CD 10_1101-2021_01_08_425864 216 22 ; ; : 10_1101-2021_01_08_425864 216 23 * * NFP 10_1101-2021_01_08_425864 216 24 * * NFP 10_1101-2021_01_08_425864 216 25 p p NN 10_1101-2021_01_08_425864 216 26 value value NN 10_1101-2021_01_08_425864 216 27 < < XX 10_1101-2021_01_08_425864 216 28 0.01 0.01 XX 10_1101-2021_01_08_425864 216 29 ; ; : 10_1101-2021_01_08_425864 216 30 and and CC 10_1101-2021_01_08_425864 216 31 * * NFP 10_1101-2021_01_08_425864 216 32 p p NN 10_1101-2021_01_08_425864 216 33 value value NN 10_1101-2021_01_08_425864 216 34 < < XX 10_1101-2021_01_08_425864 216 35 0.05 0.05 CD 10_1101-2021_01_08_425864 216 36 . . . 10_1101-2021_01_08_425864 217 1 For for IN 10_1101-2021_01_08_425864 217 2 p p NN 10_1101-2021_01_08_425864 217 3 value value NN 10_1101-2021_01_08_425864 217 4 > > XX 10_1101-2021_01_08_425864 217 5 0.05 0.05 CD 10_1101-2021_01_08_425864 217 6 , , , 10_1101-2021_01_08_425864 217 7 356 356 CD 10_1101-2021_01_08_425864 217 8 we -PRON- PRP 10_1101-2021_01_08_425864 217 9 consider consider VBP 10_1101-2021_01_08_425864 217 10 the the DT 10_1101-2021_01_08_425864 217 11 differences difference NNS 10_1101-2021_01_08_425864 217 12 not not RB 10_1101-2021_01_08_425864 217 13 significant significant JJ 10_1101-2021_01_08_425864 217 14 ( ( -LRB- 10_1101-2021_01_08_425864 217 15 ns ns NNP 10_1101-2021_01_08_425864 217 16 ) ) -RRB- 10_1101-2021_01_08_425864 217 17 . . . 10_1101-2021_01_08_425864 218 1 357 357 CD 10_1101-2021_01_08_425864 218 2 358 358 CD 10_1101-2021_01_08_425864 218 3 Epimastigote epimastigote JJ 10_1101-2021_01_08_425864 218 4 forms form NNS 10_1101-2021_01_08_425864 218 5 use use VBP 10_1101-2021_01_08_425864 218 6 endogenous endogenous JJ 10_1101-2021_01_08_425864 218 7 fatty fatty NN 10_1101-2021_01_08_425864 218 8 acids acid NNS 10_1101-2021_01_08_425864 218 9 to to TO 10_1101-2021_01_08_425864 218 10 support support VB 10_1101-2021_01_08_425864 218 11 growth growth NN 10_1101-2021_01_08_425864 218 12 after after IN 10_1101-2021_01_08_425864 218 13 glucose glucose NN 10_1101-2021_01_08_425864 218 14 exhaustion exhaustion NN 10_1101-2021_01_08_425864 218 15 359 359 CD 10_1101-2021_01_08_425864 218 16 From from IN 10_1101-2021_01_08_425864 218 17 the the DT 10_1101-2021_01_08_425864 218 18 previous previous JJ 10_1101-2021_01_08_425864 218 19 results result NNS 10_1101-2021_01_08_425864 218 20 , , , 10_1101-2021_01_08_425864 218 21 we -PRON- PRP 10_1101-2021_01_08_425864 218 22 learned learn VBD 10_1101-2021_01_08_425864 218 23 that that IN 10_1101-2021_01_08_425864 218 24 under under IN 10_1101-2021_01_08_425864 218 25 glucose glucose NN 10_1101-2021_01_08_425864 218 26 deprivation deprivation NN 10_1101-2021_01_08_425864 218 27 , , , 10_1101-2021_01_08_425864 218 28 TAGs tag NNS 10_1101-2021_01_08_425864 218 29 are be VBP 10_1101-2021_01_08_425864 218 30 taken take VBN 10_1101-2021_01_08_425864 218 31 up up RP 10_1101-2021_01_08_425864 218 32 by by IN 10_1101-2021_01_08_425864 218 33 360 360 CD 10_1101-2021_01_08_425864 218 34 the the DT 10_1101-2021_01_08_425864 218 35 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 218 36 , , , 10_1101-2021_01_08_425864 218 37 and and CC 10_1101-2021_01_08_425864 218 38 internally internally RB 10_1101-2021_01_08_425864 218 39 stored store VBD 10_1101-2021_01_08_425864 218 40 fatty fatty NN 10_1101-2021_01_08_425864 218 41 acids acid NNS 10_1101-2021_01_08_425864 218 42 are be VBP 10_1101-2021_01_08_425864 218 43 mobilized mobilize VBN 10_1101-2021_01_08_425864 218 44 . . . 10_1101-2021_01_08_425864 219 1 However however RB 10_1101-2021_01_08_425864 219 2 , , , 10_1101-2021_01_08_425864 219 3 to to IN 10_1101-2021_01_08_425864 219 4 date date NN 10_1101-2021_01_08_425864 219 5 , , , 10_1101-2021_01_08_425864 219 6 we -PRON- PRP 10_1101-2021_01_08_425864 219 7 did do VBD 10_1101-2021_01_08_425864 219 8 not not RB 10_1101-2021_01_08_425864 219 9 361 361 CD 10_1101-2021_01_08_425864 219 10 provide provide VB 10_1101-2021_01_08_425864 219 11 any any DT 10_1101-2021_01_08_425864 219 12 evidence evidence NN 10_1101-2021_01_08_425864 219 13 pointing point VBG 10_1101-2021_01_08_425864 219 14 to to IN 10_1101-2021_01_08_425864 219 15 their -PRON- PRP$ 10_1101-2021_01_08_425864 219 16 use use NN 10_1101-2021_01_08_425864 219 17 as as IN 10_1101-2021_01_08_425864 219 18 reduced reduced JJ 10_1101-2021_01_08_425864 219 19 carbon carbon NN 10_1101-2021_01_08_425864 219 20 sources source NNS 10_1101-2021_01_08_425864 219 21 . . . 10_1101-2021_01_08_425864 220 1 To to TO 10_1101-2021_01_08_425864 220 2 confirm confirm VB 10_1101-2021_01_08_425864 220 3 this this DT 10_1101-2021_01_08_425864 220 4 idea idea NN 10_1101-2021_01_08_425864 220 5 , , , 10_1101-2021_01_08_425864 220 6 362 362 CD 10_1101-2021_01_08_425864 220 7 exponentially exponentially RB 10_1101-2021_01_08_425864 220 8 proliferating proliferating NN 10_1101-2021_01_08_425864 220 9 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 220 10 were be VBD 10_1101-2021_01_08_425864 220 11 incubated incubate VBN 10_1101-2021_01_08_425864 220 12 in in IN 10_1101-2021_01_08_425864 220 13 PBS PBS NNP 10_1101-2021_01_08_425864 220 14 supplemented supplement VBN 10_1101-2021_01_08_425864 220 15 with with IN 10_1101-2021_01_08_425864 220 16 palmitate palmitate NN 10_1101-2021_01_08_425864 220 17 and and CC 10_1101-2021_01_08_425864 220 18 363 363 CD 10_1101-2021_01_08_425864 220 19 14C 14c CD 10_1101-2021_01_08_425864 220 20 - - HYPH 10_1101-2021_01_08_425864 220 21 U u NN 10_1101-2021_01_08_425864 220 22 - - HYPH 10_1101-2021_01_08_425864 220 23 glucose glucose NN 10_1101-2021_01_08_425864 220 24 , , , 10_1101-2021_01_08_425864 220 25 or or CC 10_1101-2021_01_08_425864 220 26 reciprocally reciprocally RB 10_1101-2021_01_08_425864 220 27 , , , 10_1101-2021_01_08_425864 220 28 glucose glucose NN 10_1101-2021_01_08_425864 220 29 and and CC 10_1101-2021_01_08_425864 220 30 14C 14c NN 10_1101-2021_01_08_425864 220 31 - - HYPH 10_1101-2021_01_08_425864 220 32 U u NN 10_1101-2021_01_08_425864 220 33 - - HYPH 10_1101-2021_01_08_425864 220 34 palmitate palmitate NN 10_1101-2021_01_08_425864 220 35 . . . 10_1101-2021_01_08_425864 221 1 In in IN 10_1101-2021_01_08_425864 221 2 both both DT 10_1101-2021_01_08_425864 221 3 cases case NNS 10_1101-2021_01_08_425864 221 4 , , , 10_1101-2021_01_08_425864 221 5 the the DT 10_1101-2021_01_08_425864 221 6 production production NN 10_1101-2021_01_08_425864 221 7 of of IN 10_1101-2021_01_08_425864 221 8 14C- 14c- CD 10_1101-2021_01_08_425864 221 9 364 364 CD 10_1101-2021_01_08_425864 221 10 labelled label VBN 10_1101-2021_01_08_425864 221 11 CO2 CO2 NNP 10_1101-2021_01_08_425864 221 12 was be VBD 10_1101-2021_01_08_425864 221 13 quantified quantify VBN 10_1101-2021_01_08_425864 221 14 . . . 10_1101-2021_01_08_425864 222 1 The the DT 10_1101-2021_01_08_425864 222 2 presence presence NN 10_1101-2021_01_08_425864 222 3 of of IN 10_1101-2021_01_08_425864 222 4 5 5 CD 10_1101-2021_01_08_425864 222 5 mM mM NNP 10_1101-2021_01_08_425864 222 6 glucose glucose NN 10_1101-2021_01_08_425864 222 7 diminished diminish VBD 10_1101-2021_01_08_425864 222 8 the the DT 10_1101-2021_01_08_425864 222 9 release release NN 10_1101-2021_01_08_425864 222 10 of of IN 10_1101-2021_01_08_425864 222 11 14CO2 14co2 CD 10_1101-2021_01_08_425864 222 12 from from IN 10_1101-2021_01_08_425864 222 13 365 365 CD 10_1101-2021_01_08_425864 222 14 14C 14C NNP 10_1101-2021_01_08_425864 222 15 - - HYPH 10_1101-2021_01_08_425864 222 16 U u NN 10_1101-2021_01_08_425864 222 17 - - HYPH 10_1101-2021_01_08_425864 222 18 palmitate palmitate NN 10_1101-2021_01_08_425864 222 19 by by IN 10_1101-2021_01_08_425864 222 20 90 90 CD 10_1101-2021_01_08_425864 222 21 % % NN 10_1101-2021_01_08_425864 222 22 while while IN 10_1101-2021_01_08_425864 222 23 the the DT 10_1101-2021_01_08_425864 222 24 presence presence NN 10_1101-2021_01_08_425864 222 25 of of IN 10_1101-2021_01_08_425864 222 26 palmitate palmitate NN 10_1101-2021_01_08_425864 222 27 did do VBD 10_1101-2021_01_08_425864 222 28 not not RB 10_1101-2021_01_08_425864 222 29 interfere interfere VB 10_1101-2021_01_08_425864 222 30 with with IN 10_1101-2021_01_08_425864 222 31 the the DT 10_1101-2021_01_08_425864 222 32 production production NN 10_1101-2021_01_08_425864 222 33 of of IN 10_1101-2021_01_08_425864 222 34 14CO2 14co2 CD 10_1101-2021_01_08_425864 222 35 366 366 CD 10_1101-2021_01_08_425864 222 36 from from IN 10_1101-2021_01_08_425864 222 37 14C 14c CD 10_1101-2021_01_08_425864 222 38 - - HYPH 10_1101-2021_01_08_425864 222 39 U u NN 10_1101-2021_01_08_425864 222 40 - - HYPH 10_1101-2021_01_08_425864 222 41 glucose glucose NN 10_1101-2021_01_08_425864 222 42 ( ( -LRB- 10_1101-2021_01_08_425864 222 43 Fig fig NN 10_1101-2021_01_08_425864 222 44 4 4 CD 10_1101-2021_01_08_425864 222 45 ) ) -RRB- 10_1101-2021_01_08_425864 222 46 . . . 10_1101-2021_01_08_425864 223 1 Taken take VBN 10_1101-2021_01_08_425864 223 2 together together RB 10_1101-2021_01_08_425864 223 3 , , , 10_1101-2021_01_08_425864 223 4 our -PRON- PRP$ 10_1101-2021_01_08_425864 223 5 results result NNS 10_1101-2021_01_08_425864 223 6 show show VBP 10_1101-2021_01_08_425864 223 7 that that WDT 10_1101-2021_01_08_425864 223 8 glucose glucose VBP 10_1101-2021_01_08_425864 223 9 inhibits inhibit VBZ 10_1101-2021_01_08_425864 223 10 TAGs tag NNS 10_1101-2021_01_08_425864 223 11 and and CC 10_1101-2021_01_08_425864 223 12 fatty fatty VBP 10_1101-2021_01_08_425864 223 13 367 367 CD 10_1101-2021_01_08_425864 223 14 acid acid NN 10_1101-2021_01_08_425864 223 15 consumption consumption NN 10_1101-2021_01_08_425864 223 16 , , , 10_1101-2021_01_08_425864 223 17 and and CC 10_1101-2021_01_08_425864 223 18 after after IN 10_1101-2021_01_08_425864 223 19 glucose glucose VB 10_1101-2021_01_08_425864 223 20 exhaustion exhaustion NN 10_1101-2021_01_08_425864 223 21 , , , 10_1101-2021_01_08_425864 223 22 a a DT 10_1101-2021_01_08_425864 223 23 metabolic metabolic JJ 10_1101-2021_01_08_425864 223 24 switch switch NN 10_1101-2021_01_08_425864 223 25 occurs occur VBZ 10_1101-2021_01_08_425864 223 26 towards towards IN 10_1101-2021_01_08_425864 223 27 the the DT 10_1101-2021_01_08_425864 223 28 oxidation oxidation NN 10_1101-2021_01_08_425864 223 29 of of IN 10_1101-2021_01_08_425864 223 30 368 368 CD 10_1101-2021_01_08_425864 223 31 internally internally RB 10_1101-2021_01_08_425864 223 32 stored store VBN 10_1101-2021_01_08_425864 223 33 fatty fatty NN 10_1101-2021_01_08_425864 223 34 acids acid NNS 10_1101-2021_01_08_425864 223 35 . . . 10_1101-2021_01_08_425864 224 1 369 369 CD 10_1101-2021_01_08_425864 224 2 370 370 CD 10_1101-2021_01_08_425864 224 3 Figure figure NN 10_1101-2021_01_08_425864 224 4 4 4 CD 10_1101-2021_01_08_425864 224 5 . . . 10_1101-2021_01_08_425864 225 1 Glucose Glucose NNP 10_1101-2021_01_08_425864 225 2 metabolism metabolism NN 10_1101-2021_01_08_425864 225 3 inhibits inhibit VBZ 10_1101-2021_01_08_425864 225 4 FAO FAO NNP 10_1101-2021_01_08_425864 225 5 . . . 10_1101-2021_01_08_425864 226 1 Parasites parasite NNS 10_1101-2021_01_08_425864 226 2 were be VBD 10_1101-2021_01_08_425864 226 3 incubated incubate VBN 10_1101-2021_01_08_425864 226 4 in in IN 10_1101-2021_01_08_425864 226 5 the the DT 10_1101-2021_01_08_425864 226 6 presence presence NN 10_1101-2021_01_08_425864 226 7 of of IN 10_1101-2021_01_08_425864 226 8 14C 14C NNP 10_1101-2021_01_08_425864 226 9 - - HYPH 10_1101-2021_01_08_425864 226 10 U- u- NN 10_1101-2021_01_08_425864 226 11 371 371 CD 10_1101-2021_01_08_425864 226 12 palmitate palmitate NN 10_1101-2021_01_08_425864 226 13 + + CC 10_1101-2021_01_08_425864 226 14 5 5 CD 10_1101-2021_01_08_425864 226 15 mM mm NN 10_1101-2021_01_08_425864 226 16 glucose glucose NN 10_1101-2021_01_08_425864 226 17 and and CC 10_1101-2021_01_08_425864 226 18 14C 14c NN 10_1101-2021_01_08_425864 226 19 - - HYPH 10_1101-2021_01_08_425864 226 20 U u NN 10_1101-2021_01_08_425864 226 21 - - HYPH 10_1101-2021_01_08_425864 226 22 glucose glucose NN 10_1101-2021_01_08_425864 226 23 + + SYM 10_1101-2021_01_08_425864 226 24 0.1 0.1 CD 10_1101-2021_01_08_425864 226 25 mM mm NN 10_1101-2021_01_08_425864 226 26 palmitate palmitate NN 10_1101-2021_01_08_425864 226 27 in in IN 10_1101-2021_01_08_425864 226 28 PBS PBS NNP 10_1101-2021_01_08_425864 226 29 . . . 10_1101-2021_01_08_425864 227 1 14CO2 14co2 CD 10_1101-2021_01_08_425864 227 2 production production NN 10_1101-2021_01_08_425864 227 3 from from IN 10_1101-2021_01_08_425864 227 4 372 372 CD 10_1101-2021_01_08_425864 227 5 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 227 6 incubated incubate VBN 10_1101-2021_01_08_425864 227 7 in in IN 10_1101-2021_01_08_425864 227 8 PBS PBS NNP 10_1101-2021_01_08_425864 227 9 . . . 10_1101-2021_01_08_425864 228 1 The the DT 10_1101-2021_01_08_425864 228 2 14CO2 14co2 CD 10_1101-2021_01_08_425864 228 3 was be VBD 10_1101-2021_01_08_425864 228 4 captured capture VBN 10_1101-2021_01_08_425864 228 5 after after IN 10_1101-2021_01_08_425864 228 6 120 120 CD 10_1101-2021_01_08_425864 228 7 min min NN 10_1101-2021_01_08_425864 228 8 of of IN 10_1101-2021_01_08_425864 228 9 incubation incubation NN 10_1101-2021_01_08_425864 228 10 . . . 10_1101-2021_01_08_425864 229 1 The the DT 10_1101-2021_01_08_425864 229 2 373 373 CD 10_1101-2021_01_08_425864 229 3 experiments experiment NNS 10_1101-2021_01_08_425864 229 4 were be VBD 10_1101-2021_01_08_425864 229 5 performed perform VBN 10_1101-2021_01_08_425864 229 6 in in IN 10_1101-2021_01_08_425864 229 7 triplicates triplicate NNS 10_1101-2021_01_08_425864 229 8 . . . 10_1101-2021_01_08_425864 230 1 Statistical statistical JJ 10_1101-2021_01_08_425864 230 2 analysis analysis NN 10_1101-2021_01_08_425864 230 3 was be VBD 10_1101-2021_01_08_425864 230 4 performed perform VBN 10_1101-2021_01_08_425864 230 5 with with IN 10_1101-2021_01_08_425864 230 6 one one CD 10_1101-2021_01_08_425864 230 7 - - HYPH 10_1101-2021_01_08_425864 230 8 way way NN 10_1101-2021_01_08_425864 230 9 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 230 10 374 374 CD 10_1101-2021_01_08_425864 230 11 followed follow VBN 10_1101-2021_01_08_425864 230 12 by by IN 10_1101-2021_01_08_425864 230 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 230 14 's 's POS 10_1101-2021_01_08_425864 230 15 post post JJ 10_1101-2021_01_08_425864 230 16 - - JJ 10_1101-2021_01_08_425864 230 17 test test JJ 10_1101-2021_01_08_425864 230 18 p p NN 10_1101-2021_01_08_425864 230 19 < < XX 10_1101-2021_01_08_425864 230 20 0.05 0.05 XX 10_1101-2021_01_08_425864 230 21 using use VBG 10_1101-2021_01_08_425864 230 22 the the DT 10_1101-2021_01_08_425864 230 23 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 230 24 Prism Prism NNP 10_1101-2021_01_08_425864 230 25 8.0.2 8.0.2 NNP 10_1101-2021_01_08_425864 230 26 software software NN 10_1101-2021_01_08_425864 230 27 program program NN 10_1101-2021_01_08_425864 230 28 . . . 10_1101-2021_01_08_425864 231 1 We -PRON- PRP 10_1101-2021_01_08_425864 231 2 375 375 CD 10_1101-2021_01_08_425864 231 3 represent represent VBP 10_1101-2021_01_08_425864 231 4 the the DT 10_1101-2021_01_08_425864 231 5 level level NN 10_1101-2021_01_08_425864 231 6 of of IN 10_1101-2021_01_08_425864 231 7 statistical statistical JJ 10_1101-2021_01_08_425864 231 8 significance significance NN 10_1101-2021_01_08_425864 231 9 in in IN 10_1101-2021_01_08_425864 231 10 this this DT 10_1101-2021_01_08_425864 231 11 figure figure NN 10_1101-2021_01_08_425864 231 12 as as IN 10_1101-2021_01_08_425864 231 13 follows follow VBZ 10_1101-2021_01_08_425864 231 14 : : : 10_1101-2021_01_08_425864 231 15 * * NFP 10_1101-2021_01_08_425864 231 16 * * NFP 10_1101-2021_01_08_425864 231 17 * * NFP 10_1101-2021_01_08_425864 231 18 p p NN 10_1101-2021_01_08_425864 231 19 value value NN 10_1101-2021_01_08_425864 231 20 < < XX 10_1101-2021_01_08_425864 231 21 0.001 0.001 CD 10_1101-2021_01_08_425864 231 22 ; ; : 10_1101-2021_01_08_425864 231 23 * * NFP 10_1101-2021_01_08_425864 231 24 * * NFP 10_1101-2021_01_08_425864 231 25 p p NN 10_1101-2021_01_08_425864 231 26 value value NN 10_1101-2021_01_08_425864 231 27 376 376 CD 10_1101-2021_01_08_425864 231 28 < < XX 10_1101-2021_01_08_425864 231 29 0.01 0.01 CD 10_1101-2021_01_08_425864 231 30 ; ; : 10_1101-2021_01_08_425864 231 31 and and CC 10_1101-2021_01_08_425864 231 32 * * NFP 10_1101-2021_01_08_425864 231 33 p p NN 10_1101-2021_01_08_425864 231 34 value value NN 10_1101-2021_01_08_425864 231 35 < < XX 10_1101-2021_01_08_425864 231 36 0.05 0.05 CD 10_1101-2021_01_08_425864 231 37 . . . 10_1101-2021_01_08_425864 232 1 For for IN 10_1101-2021_01_08_425864 232 2 p p NN 10_1101-2021_01_08_425864 232 3 value value NN 10_1101-2021_01_08_425864 232 4 > > XX 10_1101-2021_01_08_425864 232 5 0.05 0.05 CD 10_1101-2021_01_08_425864 232 6 , , , 10_1101-2021_01_08_425864 232 7 we -PRON- PRP 10_1101-2021_01_08_425864 232 8 consider consider VBP 10_1101-2021_01_08_425864 232 9 the the DT 10_1101-2021_01_08_425864 232 10 differences difference NNS 10_1101-2021_01_08_425864 232 11 not not RB 10_1101-2021_01_08_425864 232 12 significant significant JJ 10_1101-2021_01_08_425864 232 13 ( ( -LRB- 10_1101-2021_01_08_425864 232 14 ns ns NNP 10_1101-2021_01_08_425864 232 15 ) ) -RRB- 10_1101-2021_01_08_425864 232 16 . . . 10_1101-2021_01_08_425864 233 1 377 377 CD 10_1101-2021_01_08_425864 233 2 378 378 CD 10_1101-2021_01_08_425864 233 3 To to TO 10_1101-2021_01_08_425864 233 4 monitor monitor VB 10_1101-2021_01_08_425864 233 5 the the DT 10_1101-2021_01_08_425864 233 6 dynamics dynamic NNS 10_1101-2021_01_08_425864 233 7 of of IN 10_1101-2021_01_08_425864 233 8 use use NN 10_1101-2021_01_08_425864 233 9 or or CC 10_1101-2021_01_08_425864 233 10 accumulation accumulation NN 10_1101-2021_01_08_425864 233 11 of of IN 10_1101-2021_01_08_425864 233 12 fatty fatty NN 10_1101-2021_01_08_425864 233 13 acids acid NNS 10_1101-2021_01_08_425864 233 14 in in IN 10_1101-2021_01_08_425864 233 15 lipid lipid NN 10_1101-2021_01_08_425864 233 16 droplets droplet NNS 10_1101-2021_01_08_425864 233 17 , , , 10_1101-2021_01_08_425864 233 18 we -PRON- PRP 10_1101-2021_01_08_425864 233 19 used use VBD 10_1101-2021_01_08_425864 233 20 as as IN 10_1101-2021_01_08_425864 233 21 a a DT 10_1101-2021_01_08_425864 233 22 379 379 CD 10_1101-2021_01_08_425864 233 23 probe probe NN 10_1101-2021_01_08_425864 233 24 a a DT 10_1101-2021_01_08_425864 233 25 fluorescent fluorescent NN 10_1101-2021_01_08_425864 233 26 fatty fatty NN 10_1101-2021_01_08_425864 233 27 acid acid NN 10_1101-2021_01_08_425864 233 28 analogue analogue NN 10_1101-2021_01_08_425864 233 29 called call VBN 10_1101-2021_01_08_425864 233 30 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 233 31 500/510 500/510 NNP 10_1101-2021_01_08_425864 233 32 C1-C12 C1-C12 NNP 10_1101-2021_01_08_425864 233 33 . . . 10_1101-2021_01_08_425864 234 1 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 234 2 shifts shift VBZ 10_1101-2021_01_08_425864 234 3 its -PRON- PRP$ 10_1101-2021_01_08_425864 234 4 380 380 CD 10_1101-2021_01_08_425864 234 5 fluorescence fluorescence NN 10_1101-2021_01_08_425864 234 6 from from IN 10_1101-2021_01_08_425864 234 7 red red NN 10_1101-2021_01_08_425864 234 8 to to IN 10_1101-2021_01_08_425864 234 9 green green NN 10_1101-2021_01_08_425864 234 10 upon upon IN 10_1101-2021_01_08_425864 234 11 the the DT 10_1101-2021_01_08_425864 234 12 uptake uptake NN 10_1101-2021_01_08_425864 234 13 and and CC 10_1101-2021_01_08_425864 234 14 catabolism catabolism NN 10_1101-2021_01_08_425864 234 15 of of IN 10_1101-2021_01_08_425864 234 16 fatty fatty NN 10_1101-2021_01_08_425864 234 17 acids acid NNS 10_1101-2021_01_08_425864 234 18 , , , 10_1101-2021_01_08_425864 234 19 and and CC 10_1101-2021_01_08_425864 234 20 from from IN 10_1101-2021_01_08_425864 234 21 green green NN 10_1101-2021_01_08_425864 234 22 to to IN 10_1101-2021_01_08_425864 234 23 red red NNP 10_1101-2021_01_08_425864 234 24 381 381 CD 10_1101-2021_01_08_425864 234 25 when when WRB 10_1101-2021_01_08_425864 234 26 fatty fatty NN 10_1101-2021_01_08_425864 234 27 acids acid NNS 10_1101-2021_01_08_425864 234 28 are be VBP 10_1101-2021_01_08_425864 234 29 accumulated accumulate VBN 10_1101-2021_01_08_425864 234 30 in in IN 10_1101-2021_01_08_425864 234 31 the the DT 10_1101-2021_01_08_425864 234 32 lipid lipid NN 10_1101-2021_01_08_425864 234 33 droplets droplet NNS 10_1101-2021_01_08_425864 234 34 . . . 10_1101-2021_01_08_425864 235 1 Parasites parasite NNS 10_1101-2021_01_08_425864 235 2 collected collect VBD 10_1101-2021_01_08_425864 235 3 at at IN 10_1101-2021_01_08_425864 235 4 the the DT 10_1101-2021_01_08_425864 235 5 mid mid JJ 10_1101-2021_01_08_425864 235 6 and and CC 10_1101-2021_01_08_425864 235 7 late late JJ 10_1101-2021_01_08_425864 235 8 382 382 CD 10_1101-2021_01_08_425864 235 9 exponential exponential JJ 10_1101-2021_01_08_425864 235 10 proliferation proliferation NN 10_1101-2021_01_08_425864 235 11 phases phase NNS 10_1101-2021_01_08_425864 235 12 and and CC 10_1101-2021_01_08_425864 235 13 the the DT 10_1101-2021_01_08_425864 235 14 stationary stationary JJ 10_1101-2021_01_08_425864 235 15 phase phase NN 10_1101-2021_01_08_425864 235 16 were be VBD 10_1101-2021_01_08_425864 235 17 incubated incubate VBN 10_1101-2021_01_08_425864 235 18 with with IN 10_1101-2021_01_08_425864 235 19 1 1 CD 10_1101-2021_01_08_425864 235 20 μM μM NNP 10_1101-2021_01_08_425864 235 21 BODIPY bodipy RB 10_1101-2021_01_08_425864 235 22 383 383 CD 10_1101-2021_01_08_425864 235 23 500/510 500/510 NNP 10_1101-2021_01_08_425864 235 24 C1-C12 C1-C12 NNP 10_1101-2021_01_08_425864 235 25 for for IN 10_1101-2021_01_08_425864 235 26 16 16 CD 10_1101-2021_01_08_425864 235 27 h h NNS 10_1101-2021_01_08_425864 235 28 , , , 10_1101-2021_01_08_425864 235 29 before before IN 10_1101-2021_01_08_425864 235 30 fluorescence fluorescence NN 10_1101-2021_01_08_425864 235 31 determination determination NN 10_1101-2021_01_08_425864 235 32 by by IN 10_1101-2021_01_08_425864 235 33 flow flow NN 10_1101-2021_01_08_425864 235 34 cytometry cytometry NN 10_1101-2021_01_08_425864 235 35 ( ( -LRB- 10_1101-2021_01_08_425864 235 36 Figs fig NNS 10_1101-2021_01_08_425864 235 37 5A 5a RB 10_1101-2021_01_08_425864 235 38 , , , 10_1101-2021_01_08_425864 235 39 5B 5b JJ 10_1101-2021_01_08_425864 235 40 and and CC 10_1101-2021_01_08_425864 235 41 5C 5c NN 10_1101-2021_01_08_425864 235 42 ) ) -RRB- 10_1101-2021_01_08_425864 235 43 . . . 10_1101-2021_01_08_425864 236 1 384 384 CD 10_1101-2021_01_08_425864 236 2 The the DT 10_1101-2021_01_08_425864 236 3 fluorescence fluorescence NN 10_1101-2021_01_08_425864 236 4 values value NNS 10_1101-2021_01_08_425864 236 5 increased increase VBD 10_1101-2021_01_08_425864 236 6 with with IN 10_1101-2021_01_08_425864 236 7 the the DT 10_1101-2021_01_08_425864 236 8 harvesting harvesting NN 10_1101-2021_01_08_425864 236 9 time time NN 10_1101-2021_01_08_425864 236 10 ( ( -LRB- 10_1101-2021_01_08_425864 236 11 and and CC 10_1101-2021_01_08_425864 236 12 therefore therefore RB 10_1101-2021_01_08_425864 236 13 , , , 10_1101-2021_01_08_425864 236 14 with with IN 10_1101-2021_01_08_425864 236 15 the the DT 10_1101-2021_01_08_425864 236 16 glucose glucose NN 10_1101-2021_01_08_425864 236 17 385 385 CD 10_1101-2021_01_08_425864 236 18 depletion depletion NN 10_1101-2021_01_08_425864 236 19 ) ) -RRB- 10_1101-2021_01_08_425864 236 20 , , , 10_1101-2021_01_08_425864 236 21 indicating indicate VBG 10_1101-2021_01_08_425864 236 22 the the DT 10_1101-2021_01_08_425864 236 23 increased increased JJ 10_1101-2021_01_08_425864 236 24 uptake uptake NN 10_1101-2021_01_08_425864 236 25 and and CC 10_1101-2021_01_08_425864 236 26 use use NN 10_1101-2021_01_08_425864 236 27 of of IN 10_1101-2021_01_08_425864 236 28 fatty fatty NN 10_1101-2021_01_08_425864 236 29 acids acid NNS 10_1101-2021_01_08_425864 236 30 as as IN 10_1101-2021_01_08_425864 236 31 substrates substrate NNS 10_1101-2021_01_08_425864 236 32 by by IN 10_1101-2021_01_08_425864 236 33 a a DT 10_1101-2021_01_08_425864 236 34 fatty fatty NN 10_1101-2021_01_08_425864 236 35 acyl acyl NN 10_1101-2021_01_08_425864 236 36 - - HYPH 10_1101-2021_01_08_425864 236 37 CoA CoA NNP 10_1101-2021_01_08_425864 236 38 386 386 CD 10_1101-2021_01_08_425864 236 39 synthetase synthetase NN 10_1101-2021_01_08_425864 236 40 . . . 10_1101-2021_01_08_425864 237 1 These these DT 10_1101-2021_01_08_425864 237 2 data datum NNS 10_1101-2021_01_08_425864 237 3 were be VBD 10_1101-2021_01_08_425864 237 4 confirmed confirm VBN 10_1101-2021_01_08_425864 237 5 by by IN 10_1101-2021_01_08_425864 237 6 fluorescence fluorescence NN 10_1101-2021_01_08_425864 237 7 microscopy microscopy JJ 10_1101-2021_01_08_425864 237 8 ( ( -LRB- 10_1101-2021_01_08_425864 237 9 Fig Fig NNP 10_1101-2021_01_08_425864 237 10 5D 5d NN 10_1101-2021_01_08_425864 237 11 ) ) -RRB- 10_1101-2021_01_08_425864 237 12 . . . 10_1101-2021_01_08_425864 238 1 Interestingly interestingly RB 10_1101-2021_01_08_425864 238 2 , , , 10_1101-2021_01_08_425864 238 3 parasites parasite VBZ 10_1101-2021_01_08_425864 238 4 387 387 CD 10_1101-2021_01_08_425864 238 5 in in IN 10_1101-2021_01_08_425864 238 6 stationary stationary JJ 10_1101-2021_01_08_425864 238 7 phase phase NN 10_1101-2021_01_08_425864 238 8 showed show VBD 10_1101-2021_01_08_425864 238 9 an an DT 10_1101-2021_01_08_425864 238 10 accumulation accumulation NN 10_1101-2021_01_08_425864 238 11 of of IN 10_1101-2021_01_08_425864 238 12 activated activated JJ 10_1101-2021_01_08_425864 238 13 fatty fatty NN 10_1101-2021_01_08_425864 238 14 acids acid NNS 10_1101-2021_01_08_425864 238 15 in in IN 10_1101-2021_01_08_425864 238 16 spots spot NNS 10_1101-2021_01_08_425864 238 17 along along IN 10_1101-2021_01_08_425864 238 18 the the DT 10_1101-2021_01_08_425864 238 19 cell cell NN 10_1101-2021_01_08_425864 238 20 . . . 10_1101-2021_01_08_425864 239 1 However however RB 10_1101-2021_01_08_425864 239 2 , , , 10_1101-2021_01_08_425864 239 3 388 388 CD 10_1101-2021_01_08_425864 239 4 the the DT 10_1101-2021_01_08_425864 239 5 number number NN 10_1101-2021_01_08_425864 239 6 of of IN 10_1101-2021_01_08_425864 239 7 lipid lipid NN 10_1101-2021_01_08_425864 239 8 droplets droplet NNS 10_1101-2021_01_08_425864 239 9 increased increase VBD 10_1101-2021_01_08_425864 239 10 upon upon IN 10_1101-2021_01_08_425864 239 11 parasite parasite NN 10_1101-2021_01_08_425864 239 12 proliferation proliferation NN 10_1101-2021_01_08_425864 239 13 ( ( -LRB- 10_1101-2021_01_08_425864 239 14 Figs figs NN 10_1101-2021_01_08_425864 239 15 6A 6a NN 10_1101-2021_01_08_425864 239 16 , , , 10_1101-2021_01_08_425864 239 17 6B 6b JJ 10_1101-2021_01_08_425864 239 18 6C 6c NN 10_1101-2021_01_08_425864 239 19 ) ) -RRB- 10_1101-2021_01_08_425864 239 20 . . . 10_1101-2021_01_08_425864 240 1 This this DT 10_1101-2021_01_08_425864 240 2 observation observation NN 10_1101-2021_01_08_425864 240 3 .CC .CC : 10_1101-2021_01_08_425864 240 4 - - : 10_1101-2021_01_08_425864 240 5 BY by IN 10_1101-2021_01_08_425864 240 6 4.0 4.0 CD 10_1101-2021_01_08_425864 240 7 International international JJ 10_1101-2021_01_08_425864 240 8 licensemade licensemade NN 10_1101-2021_01_08_425864 240 9 available available JJ 10_1101-2021_01_08_425864 240 10 under under IN 10_1101-2021_01_08_425864 240 11 a a DT 10_1101-2021_01_08_425864 240 12 ( ( -LRB- 10_1101-2021_01_08_425864 240 13 which which WDT 10_1101-2021_01_08_425864 240 14 was be VBD 10_1101-2021_01_08_425864 240 15 not not RB 10_1101-2021_01_08_425864 240 16 certified certify VBN 10_1101-2021_01_08_425864 240 17 by by IN 10_1101-2021_01_08_425864 240 18 peer peer NN 10_1101-2021_01_08_425864 240 19 review review NN 10_1101-2021_01_08_425864 240 20 ) ) -RRB- 10_1101-2021_01_08_425864 240 21 is be VBZ 10_1101-2021_01_08_425864 240 22 the the DT 10_1101-2021_01_08_425864 240 23 author author NN 10_1101-2021_01_08_425864 240 24 / / SYM 10_1101-2021_01_08_425864 240 25 funder funder NN 10_1101-2021_01_08_425864 240 26 , , , 10_1101-2021_01_08_425864 240 27 who who WP 10_1101-2021_01_08_425864 240 28 has have VBZ 10_1101-2021_01_08_425864 240 29 granted grant VBN 10_1101-2021_01_08_425864 240 30 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 240 31 a a DT 10_1101-2021_01_08_425864 240 32 license license NN 10_1101-2021_01_08_425864 240 33 to to TO 10_1101-2021_01_08_425864 240 34 display display VB 10_1101-2021_01_08_425864 240 35 the the DT 10_1101-2021_01_08_425864 240 36 preprint preprint NN 10_1101-2021_01_08_425864 240 37 in in IN 10_1101-2021_01_08_425864 240 38 perpetuity perpetuity NN 10_1101-2021_01_08_425864 240 39 . . . 10_1101-2021_01_08_425864 241 1 It -PRON- PRP 10_1101-2021_01_08_425864 241 2 is be VBZ 10_1101-2021_01_08_425864 241 3 The the DT 10_1101-2021_01_08_425864 241 4 copyright copyright NN 10_1101-2021_01_08_425864 241 5 holder holder NN 10_1101-2021_01_08_425864 241 6 for for IN 10_1101-2021_01_08_425864 241 7 this this DT 10_1101-2021_01_08_425864 241 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 241 9 version version NN 10_1101-2021_01_08_425864 241 10 posted post VBD 10_1101-2021_01_08_425864 241 11 January January NNP 10_1101-2021_01_08_425864 241 12 8 8 CD 10_1101-2021_01_08_425864 241 13 , , , 10_1101-2021_01_08_425864 241 14 2021 2021 CD 10_1101-2021_01_08_425864 241 15 . . . 10_1101-2021_01_08_425864 241 16 ; ; : 10_1101-2021_01_08_425864 241 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 241 18 : : : 10_1101-2021_01_08_425864 241 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 241 20 preprint preprint NN 10_1101-2021_01_08_425864 241 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 241 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 241 23 389 389 CD 10_1101-2021_01_08_425864 241 24 indicates indicate VBZ 10_1101-2021_01_08_425864 241 25 that that IN 10_1101-2021_01_08_425864 241 26 not not RB 10_1101-2021_01_08_425864 241 27 only only RB 10_1101-2021_01_08_425864 241 28 fatty fatty NN 10_1101-2021_01_08_425864 241 29 acids acid NNS 10_1101-2021_01_08_425864 241 30 metabolism metabolism NN 10_1101-2021_01_08_425864 241 31 is be VBZ 10_1101-2021_01_08_425864 241 32 activated activate VBN 10_1101-2021_01_08_425864 241 33 after after IN 10_1101-2021_01_08_425864 241 34 glucose glucose NN 10_1101-2021_01_08_425864 241 35 exhaustion exhaustion NN 10_1101-2021_01_08_425864 241 36 , , , 10_1101-2021_01_08_425864 241 37 but but CC 10_1101-2021_01_08_425864 241 38 also also RB 10_1101-2021_01_08_425864 241 39 the the DT 10_1101-2021_01_08_425864 241 40 390 390 CD 10_1101-2021_01_08_425864 241 41 parasite parasite NN 10_1101-2021_01_08_425864 241 42 storage storage NN 10_1101-2021_01_08_425864 241 43 of of IN 10_1101-2021_01_08_425864 241 44 fatty fatty NN 10_1101-2021_01_08_425864 241 45 acids acid NNS 10_1101-2021_01_08_425864 241 46 into into IN 10_1101-2021_01_08_425864 241 47 lipid lipid NN 10_1101-2021_01_08_425864 241 48 droplets droplet NNS 10_1101-2021_01_08_425864 241 49 . . . 10_1101-2021_01_08_425864 242 1 391 391 CD 10_1101-2021_01_08_425864 242 2 392 392 CD 10_1101-2021_01_08_425864 242 3 393 393 CD 10_1101-2021_01_08_425864 242 4 Figure figure NN 10_1101-2021_01_08_425864 242 5 5 5 CD 10_1101-2021_01_08_425864 242 6 . . . 10_1101-2021_01_08_425864 243 1 Flow flow VB 10_1101-2021_01_08_425864 243 2 cytometry cytometry NN 10_1101-2021_01_08_425864 243 3 reveals reveal VBZ 10_1101-2021_01_08_425864 243 4 distinct distinct JJ 10_1101-2021_01_08_425864 243 5 patterns pattern NNS 10_1101-2021_01_08_425864 243 6 in in IN 10_1101-2021_01_08_425864 243 7 fatty fatty NN 10_1101-2021_01_08_425864 243 8 acid acid NN 10_1101-2021_01_08_425864 243 9 pools pool NNS 10_1101-2021_01_08_425864 243 10 during during IN 10_1101-2021_01_08_425864 243 11 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 243 12 394 394 CD 10_1101-2021_01_08_425864 243 13 growth growth NN 10_1101-2021_01_08_425864 243 14 . . . 10_1101-2021_01_08_425864 244 1 The the DT 10_1101-2021_01_08_425864 244 2 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 244 3 were be VBD 10_1101-2021_01_08_425864 244 4 treated treat VBN 10_1101-2021_01_08_425864 244 5 with with IN 10_1101-2021_01_08_425864 244 6 1 1 CD 10_1101-2021_01_08_425864 244 7 µM µM NNP 10_1101-2021_01_08_425864 244 8 of of IN 10_1101-2021_01_08_425864 244 9 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 244 10 C1-C12 C1-C12 NNP 10_1101-2021_01_08_425864 244 11 ( ( -LRB- 10_1101-2021_01_08_425864 244 12 500/510 500/510 NNP 10_1101-2021_01_08_425864 244 13 ) ) -RRB- 10_1101-2021_01_08_425864 244 14 and and CC 10_1101-2021_01_08_425864 244 15 analysed analyse VBN 10_1101-2021_01_08_425864 244 16 by by IN 10_1101-2021_01_08_425864 244 17 395 395 CD 10_1101-2021_01_08_425864 244 18 flow flow NN 10_1101-2021_01_08_425864 244 19 cytometry cytometry NN 10_1101-2021_01_08_425864 244 20 and and CC 10_1101-2021_01_08_425864 244 21 fluorescence fluorescence NN 10_1101-2021_01_08_425864 244 22 microscopy microscopy NN 10_1101-2021_01_08_425864 244 23 . . . 10_1101-2021_01_08_425864 245 1 A a LS 10_1101-2021_01_08_425864 245 2 ) ) -RRB- 10_1101-2021_01_08_425864 245 3 0 0 CD 10_1101-2021_01_08_425864 245 4 h. h. NN 10_1101-2021_01_08_425864 245 5 B B NNP 10_1101-2021_01_08_425864 245 6 ) ) -RRB- 10_1101-2021_01_08_425864 245 7 24 24 CD 10_1101-2021_01_08_425864 245 8 h. h. NN 10_1101-2021_01_08_425864 245 9 C C NNP 10_1101-2021_01_08_425864 245 10 ) ) -RRB- 10_1101-2021_01_08_425864 245 11 48 48 CD 10_1101-2021_01_08_425864 245 12 h. h. NN 10_1101-2021_01_08_425864 245 13 In in IN 10_1101-2021_01_08_425864 245 14 the the DT 10_1101-2021_01_08_425864 245 15 flow flow NN 10_1101-2021_01_08_425864 245 16 cytometry cytometry NN 10_1101-2021_01_08_425864 245 17 396 396 CD 10_1101-2021_01_08_425864 245 18 histograms histogram NNS 10_1101-2021_01_08_425864 245 19 , , , 10_1101-2021_01_08_425864 245 20 dashed dash VBD 10_1101-2021_01_08_425864 245 21 peaks peak NNS 10_1101-2021_01_08_425864 245 22 represent represent VBP 10_1101-2021_01_08_425864 245 23 unstained unstained JJ 10_1101-2021_01_08_425864 245 24 parasites parasite NNS 10_1101-2021_01_08_425864 245 25 . . . 10_1101-2021_01_08_425864 246 1 Green green JJ 10_1101-2021_01_08_425864 246 2 - - HYPH 10_1101-2021_01_08_425864 246 3 filled fill VBN 10_1101-2021_01_08_425864 246 4 peaks peak NNS 10_1101-2021_01_08_425864 246 5 represent represent NN 10_1101-2021_01_08_425864 246 6 stained stain VBN 10_1101-2021_01_08_425864 246 7 397 397 CD 10_1101-2021_01_08_425864 246 8 parasites parasite NNS 10_1101-2021_01_08_425864 246 9 . . . 10_1101-2021_01_08_425864 247 1 D D NNP 10_1101-2021_01_08_425864 247 2 ) ) -RRB- 10_1101-2021_01_08_425864 247 3 Mean Mean NNP 10_1101-2021_01_08_425864 247 4 fluorescence fluorescence NN 10_1101-2021_01_08_425864 247 5 per per IN 10_1101-2021_01_08_425864 247 6 cell cell NN 10_1101-2021_01_08_425864 247 7 . . . 10_1101-2021_01_08_425864 248 1 The the DT 10_1101-2021_01_08_425864 248 2 fluorescence fluorescence NN 10_1101-2021_01_08_425864 248 3 for for IN 10_1101-2021_01_08_425864 248 4 each each DT 10_1101-2021_01_08_425864 248 5 cell cell NN 10_1101-2021_01_08_425864 248 6 was be VBD 10_1101-2021_01_08_425864 248 7 calculated calculate VBN 10_1101-2021_01_08_425864 248 8 using use VBG 10_1101-2021_01_08_425864 248 9 ImageJ ImageJ NNP 10_1101-2021_01_08_425864 248 10 398 398 CD 10_1101-2021_01_08_425864 248 11 software software NN 10_1101-2021_01_08_425864 248 12 . . . 10_1101-2021_01_08_425864 249 1 All all PDT 10_1101-2021_01_08_425864 249 2 the the DT 10_1101-2021_01_08_425864 249 3 experiments experiment NNS 10_1101-2021_01_08_425864 249 4 were be VBD 10_1101-2021_01_08_425864 249 5 performed perform VBN 10_1101-2021_01_08_425864 249 6 in in IN 10_1101-2021_01_08_425864 249 7 triplicates triplicate NNS 10_1101-2021_01_08_425864 249 8 . . . 10_1101-2021_01_08_425864 250 1 Statistical statistical JJ 10_1101-2021_01_08_425864 250 2 analysis analysis NN 10_1101-2021_01_08_425864 250 3 was be VBD 10_1101-2021_01_08_425864 250 4 performed perform VBN 10_1101-2021_01_08_425864 250 5 with with IN 10_1101-2021_01_08_425864 250 6 399 399 CD 10_1101-2021_01_08_425864 250 7 one one CD 10_1101-2021_01_08_425864 250 8 - - HYPH 10_1101-2021_01_08_425864 250 9 way way NN 10_1101-2021_01_08_425864 250 10 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 250 11 followed follow VBN 10_1101-2021_01_08_425864 250 12 by by IN 10_1101-2021_01_08_425864 250 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 250 14 's 's POS 10_1101-2021_01_08_425864 250 15 post post JJ 10_1101-2021_01_08_425864 250 16 - - JJ 10_1101-2021_01_08_425864 250 17 test test JJ 10_1101-2021_01_08_425864 250 18 p p NN 10_1101-2021_01_08_425864 250 19 < < XX 10_1101-2021_01_08_425864 250 20 0.05 0.05 XX 10_1101-2021_01_08_425864 250 21 using use VBG 10_1101-2021_01_08_425864 250 22 the the DT 10_1101-2021_01_08_425864 250 23 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 250 24 Prism Prism NNP 10_1101-2021_01_08_425864 250 25 8.0.2 8.0.2 CD 10_1101-2021_01_08_425864 250 26 software software NN 10_1101-2021_01_08_425864 250 27 400 400 CD 10_1101-2021_01_08_425864 250 28 program program NN 10_1101-2021_01_08_425864 250 29 . . . 10_1101-2021_01_08_425864 251 1 We -PRON- PRP 10_1101-2021_01_08_425864 251 2 represent represent VBP 10_1101-2021_01_08_425864 251 3 the the DT 10_1101-2021_01_08_425864 251 4 level level NN 10_1101-2021_01_08_425864 251 5 of of IN 10_1101-2021_01_08_425864 251 6 statistical statistical JJ 10_1101-2021_01_08_425864 251 7 significance significance NN 10_1101-2021_01_08_425864 251 8 in in IN 10_1101-2021_01_08_425864 251 9 this this DT 10_1101-2021_01_08_425864 251 10 figure figure NN 10_1101-2021_01_08_425864 251 11 as as IN 10_1101-2021_01_08_425864 251 12 follows follow VBZ 10_1101-2021_01_08_425864 251 13 : : : 10_1101-2021_01_08_425864 251 14 * * NFP 10_1101-2021_01_08_425864 251 15 * * NFP 10_1101-2021_01_08_425864 251 16 * * NFP 10_1101-2021_01_08_425864 251 17 p p NN 10_1101-2021_01_08_425864 251 18 value value NN 10_1101-2021_01_08_425864 251 19 < < XX 10_1101-2021_01_08_425864 251 20 401 401 CD 10_1101-2021_01_08_425864 251 21 0.001 0.001 CD 10_1101-2021_01_08_425864 251 22 ; ; : 10_1101-2021_01_08_425864 251 23 * * NFP 10_1101-2021_01_08_425864 251 24 * * NFP 10_1101-2021_01_08_425864 251 25 p p NN 10_1101-2021_01_08_425864 251 26 value value NN 10_1101-2021_01_08_425864 251 27 < < XX 10_1101-2021_01_08_425864 251 28 0.01 0.01 XX 10_1101-2021_01_08_425864 251 29 ; ; : 10_1101-2021_01_08_425864 251 30 and and CC 10_1101-2021_01_08_425864 251 31 * * NFP 10_1101-2021_01_08_425864 251 32 p p NN 10_1101-2021_01_08_425864 251 33 value value NN 10_1101-2021_01_08_425864 251 34 < < XX 10_1101-2021_01_08_425864 251 35 0.05 0.05 CD 10_1101-2021_01_08_425864 251 36 . . . 10_1101-2021_01_08_425864 252 1 For for IN 10_1101-2021_01_08_425864 252 2 p p NN 10_1101-2021_01_08_425864 252 3 value value NN 10_1101-2021_01_08_425864 252 4 > > XX 10_1101-2021_01_08_425864 252 5 0.05 0.05 CD 10_1101-2021_01_08_425864 252 6 , , , 10_1101-2021_01_08_425864 252 7 we -PRON- PRP 10_1101-2021_01_08_425864 252 8 consider consider VBP 10_1101-2021_01_08_425864 252 9 the the DT 10_1101-2021_01_08_425864 252 10 differences difference NNS 10_1101-2021_01_08_425864 252 11 not not RB 10_1101-2021_01_08_425864 252 12 402 402 CD 10_1101-2021_01_08_425864 252 13 significant significant JJ 10_1101-2021_01_08_425864 252 14 ( ( -LRB- 10_1101-2021_01_08_425864 252 15 ns ns NNP 10_1101-2021_01_08_425864 252 16 ) ) -RRB- 10_1101-2021_01_08_425864 252 17 . . . 10_1101-2021_01_08_425864 253 1 403 403 CD 10_1101-2021_01_08_425864 253 2 404 404 CD 10_1101-2021_01_08_425864 253 3 405 405 CD 10_1101-2021_01_08_425864 253 4 Figure figure NN 10_1101-2021_01_08_425864 253 5 6 6 CD 10_1101-2021_01_08_425864 253 6 . . . 10_1101-2021_01_08_425864 254 1 Epimastigote epimastigote JJ 10_1101-2021_01_08_425864 254 2 forms form NNS 10_1101-2021_01_08_425864 254 3 accumulates accumulate VBZ 10_1101-2021_01_08_425864 254 4 fatty fatty NN 10_1101-2021_01_08_425864 254 5 acids acid NNS 10_1101-2021_01_08_425864 254 6 into into IN 10_1101-2021_01_08_425864 254 7 lipid lipid NN 10_1101-2021_01_08_425864 254 8 droplets droplet NNS 10_1101-2021_01_08_425864 254 9 during during IN 10_1101-2021_01_08_425864 254 10 growth growth NN 10_1101-2021_01_08_425864 254 11 . . . 10_1101-2021_01_08_425864 255 1 The the DT 10_1101-2021_01_08_425864 255 2 406 406 CD 10_1101-2021_01_08_425864 255 3 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 255 4 were be VBD 10_1101-2021_01_08_425864 255 5 treated treat VBN 10_1101-2021_01_08_425864 255 6 with with IN 10_1101-2021_01_08_425864 255 7 1 1 CD 10_1101-2021_01_08_425864 255 8 µM µM NNP 10_1101-2021_01_08_425864 255 9 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 255 10 C1-C12 C1-C12 NNP 10_1101-2021_01_08_425864 255 11 ( ( -LRB- 10_1101-2021_01_08_425864 255 12 500/510 500/510 NNP 10_1101-2021_01_08_425864 255 13 ) ) -RRB- 10_1101-2021_01_08_425864 255 14 and and CC 10_1101-2021_01_08_425864 255 15 analysed analyse VBN 10_1101-2021_01_08_425864 255 16 by by IN 10_1101-2021_01_08_425864 255 17 flow flow NN 10_1101-2021_01_08_425864 255 18 cytometry cytometry NN 10_1101-2021_01_08_425864 255 19 407 407 CD 10_1101-2021_01_08_425864 255 20 and and CC 10_1101-2021_01_08_425864 255 21 fluorescence fluorescence NN 10_1101-2021_01_08_425864 255 22 microscopy microscopy NN 10_1101-2021_01_08_425864 255 23 . . . 10_1101-2021_01_08_425864 256 1 A a LS 10_1101-2021_01_08_425864 256 2 ) ) -RRB- 10_1101-2021_01_08_425864 256 3 0 0 CD 10_1101-2021_01_08_425864 256 4 h. h. NN 10_1101-2021_01_08_425864 256 5 B B NNP 10_1101-2021_01_08_425864 256 6 ) ) -RRB- 10_1101-2021_01_08_425864 256 7 24 24 CD 10_1101-2021_01_08_425864 256 8 h. h. NN 10_1101-2021_01_08_425864 256 9 C C NNP 10_1101-2021_01_08_425864 256 10 ) ) -RRB- 10_1101-2021_01_08_425864 256 11 48 48 CD 10_1101-2021_01_08_425864 256 12 h. h. NN 10_1101-2021_01_08_425864 256 13 In in IN 10_1101-2021_01_08_425864 256 14 the the DT 10_1101-2021_01_08_425864 256 15 flow flow NN 10_1101-2021_01_08_425864 256 16 cytometry cytometry NN 10_1101-2021_01_08_425864 256 17 histograms histogram NNS 10_1101-2021_01_08_425864 256 18 , , , 10_1101-2021_01_08_425864 256 19 dashed dash VBD 10_1101-2021_01_08_425864 256 20 408 408 CD 10_1101-2021_01_08_425864 256 21 peaks peak NNS 10_1101-2021_01_08_425864 256 22 represent represent VBP 10_1101-2021_01_08_425864 256 23 unstained unstained JJ 10_1101-2021_01_08_425864 256 24 parasites parasite NNS 10_1101-2021_01_08_425864 256 25 . . . 10_1101-2021_01_08_425864 257 1 Yellow Yellow NNP 10_1101-2021_01_08_425864 257 2 filled fill VBD 10_1101-2021_01_08_425864 257 3 peaks peak NNS 10_1101-2021_01_08_425864 257 4 represent represent VBP 10_1101-2021_01_08_425864 257 5 positively positively RB 10_1101-2021_01_08_425864 257 6 stained stain VBN 10_1101-2021_01_08_425864 257 7 parasites parasite NNS 10_1101-2021_01_08_425864 257 8 . . . 10_1101-2021_01_08_425864 258 1 The the DT 10_1101-2021_01_08_425864 258 2 409 409 CD 10_1101-2021_01_08_425864 258 3 number number NN 10_1101-2021_01_08_425864 258 4 of of IN 10_1101-2021_01_08_425864 258 5 green green JJ 10_1101-2021_01_08_425864 258 6 / / SYM 10_1101-2021_01_08_425864 258 7 yellow yellow JJ 10_1101-2021_01_08_425864 258 8 spots spot NNS 10_1101-2021_01_08_425864 258 9 for for IN 10_1101-2021_01_08_425864 258 10 each each DT 10_1101-2021_01_08_425864 258 11 cell cell NN 10_1101-2021_01_08_425864 258 12 was be VBD 10_1101-2021_01_08_425864 258 13 calculated calculate VBN 10_1101-2021_01_08_425864 258 14 using use VBG 10_1101-2021_01_08_425864 258 15 ImageJ ImageJ NNPS 10_1101-2021_01_08_425864 258 16 software software NN 10_1101-2021_01_08_425864 258 17 . . . 10_1101-2021_01_08_425864 259 1 All all PDT 10_1101-2021_01_08_425864 259 2 the the DT 10_1101-2021_01_08_425864 259 3 experiments experiment NNS 10_1101-2021_01_08_425864 259 4 410 410 CD 10_1101-2021_01_08_425864 259 5 were be VBD 10_1101-2021_01_08_425864 259 6 performed perform VBN 10_1101-2021_01_08_425864 259 7 in in IN 10_1101-2021_01_08_425864 259 8 triplicates triplicate NNS 10_1101-2021_01_08_425864 259 9 . . . 10_1101-2021_01_08_425864 260 1 411 411 CD 10_1101-2021_01_08_425864 260 2 412 412 CD 10_1101-2021_01_08_425864 260 3 To to TO 10_1101-2021_01_08_425864 260 4 find find VB 10_1101-2021_01_08_425864 260 5 if if IN 10_1101-2021_01_08_425864 260 6 the the DT 10_1101-2021_01_08_425864 260 7 increase increase NN 10_1101-2021_01_08_425864 260 8 in in IN 10_1101-2021_01_08_425864 260 9 fatty fatty NN 10_1101-2021_01_08_425864 260 10 acid acid NN 10_1101-2021_01_08_425864 260 11 pools pool NNS 10_1101-2021_01_08_425864 260 12 is be VBZ 10_1101-2021_01_08_425864 260 13 accompanied accompany VBN 10_1101-2021_01_08_425864 260 14 by by IN 10_1101-2021_01_08_425864 260 15 a a DT 10_1101-2021_01_08_425864 260 16 change change NN 10_1101-2021_01_08_425864 260 17 in in IN 10_1101-2021_01_08_425864 260 18 the the DT 10_1101-2021_01_08_425864 260 19 levels level NNS 10_1101-2021_01_08_425864 260 20 of of IN 10_1101-2021_01_08_425864 260 21 enzymes enzyme NNS 10_1101-2021_01_08_425864 260 22 413 413 CD 10_1101-2021_01_08_425864 260 23 related relate VBN 10_1101-2021_01_08_425864 260 24 to to IN 10_1101-2021_01_08_425864 260 25 fatty fatty NN 10_1101-2021_01_08_425864 260 26 acid acid NN 10_1101-2021_01_08_425864 260 27 metabolism metabolism NN 10_1101-2021_01_08_425864 260 28 , , , 10_1101-2021_01_08_425864 260 29 we -PRON- PRP 10_1101-2021_01_08_425864 260 30 evaluated evaluate VBD 10_1101-2021_01_08_425864 260 31 the the DT 10_1101-2021_01_08_425864 260 32 specific specific JJ 10_1101-2021_01_08_425864 260 33 activities activity NNS 10_1101-2021_01_08_425864 260 34 of of IN 10_1101-2021_01_08_425864 260 35 the the DT 10_1101-2021_01_08_425864 260 36 enzymes enzyme NNS 10_1101-2021_01_08_425864 260 37 hexokinase hexokinase VBP 10_1101-2021_01_08_425864 260 38 ( ( -LRB- 10_1101-2021_01_08_425864 260 39 HK HK NNP 10_1101-2021_01_08_425864 260 40 ) ) -RRB- 10_1101-2021_01_08_425864 260 41 , , , 10_1101-2021_01_08_425864 260 42 414 414 CD 10_1101-2021_01_08_425864 260 43 which which WDT 10_1101-2021_01_08_425864 260 44 is be VBZ 10_1101-2021_01_08_425864 260 45 responsible responsible JJ 10_1101-2021_01_08_425864 260 46 for for IN 10_1101-2021_01_08_425864 260 47 the the DT 10_1101-2021_01_08_425864 260 48 initial initial JJ 10_1101-2021_01_08_425864 260 49 step step NN 10_1101-2021_01_08_425864 260 50 of of IN 10_1101-2021_01_08_425864 260 51 glycolysis glycolysis NN 10_1101-2021_01_08_425864 260 52 and and CC 10_1101-2021_01_08_425864 260 53 an an DT 10_1101-2021_01_08_425864 260 54 indicator indicator NN 10_1101-2021_01_08_425864 260 55 of of IN 10_1101-2021_01_08_425864 260 56 active active JJ 10_1101-2021_01_08_425864 260 57 glycolysis glycolysis NN 10_1101-2021_01_08_425864 260 58 ; ; : 10_1101-2021_01_08_425864 260 59 acetyl acetyl NN 10_1101-2021_01_08_425864 260 60 - - HYPH 10_1101-2021_01_08_425864 260 61 CoA CoA NNP 10_1101-2021_01_08_425864 260 62 415 415 CD 10_1101-2021_01_08_425864 260 63 carboxylase carboxylase NN 10_1101-2021_01_08_425864 260 64 ( ( -LRB- 10_1101-2021_01_08_425864 260 65 ACC ACC NNP 10_1101-2021_01_08_425864 260 66 ) ) -RRB- 10_1101-2021_01_08_425864 260 67 , , , 10_1101-2021_01_08_425864 260 68 which which WDT 10_1101-2021_01_08_425864 260 69 produces produce VBZ 10_1101-2021_01_08_425864 260 70 malonyl malonyl NN 10_1101-2021_01_08_425864 260 71 - - HYPH 10_1101-2021_01_08_425864 260 72 CoA CoA NNP 10_1101-2021_01_08_425864 260 73 for for IN 10_1101-2021_01_08_425864 260 74 fatty fatty NN 10_1101-2021_01_08_425864 260 75 acid acid NN 10_1101-2021_01_08_425864 260 76 synthesis synthesis NN 10_1101-2021_01_08_425864 260 77 and and CC 10_1101-2021_01_08_425864 260 78 carnitine carnitine NN 10_1101-2021_01_08_425864 260 79 416 416 CD 10_1101-2021_01_08_425864 260 80 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 260 81 1 1 CD 10_1101-2021_01_08_425864 260 82 ( ( -LRB- 10_1101-2021_01_08_425864 260 83 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 260 84 ) ) -RRB- 10_1101-2021_01_08_425864 260 85 , , , 10_1101-2021_01_08_425864 260 86 the the DT 10_1101-2021_01_08_425864 260 87 complex complex NN 10_1101-2021_01_08_425864 260 88 that that WDT 10_1101-2021_01_08_425864 260 89 plays play VBZ 10_1101-2021_01_08_425864 260 90 a a DT 10_1101-2021_01_08_425864 260 91 central central JJ 10_1101-2021_01_08_425864 260 92 role role NN 10_1101-2021_01_08_425864 260 93 in in IN 10_1101-2021_01_08_425864 260 94 fatty fatty NN 10_1101-2021_01_08_425864 260 95 acid acid NN 10_1101-2021_01_08_425864 260 96 oxidation oxidation NN 10_1101-2021_01_08_425864 260 97 ( ( -LRB- 10_1101-2021_01_08_425864 260 98 FAO FAO NNP 10_1101-2021_01_08_425864 260 99 ) ) -RRB- 10_1101-2021_01_08_425864 260 100 by by IN 10_1101-2021_01_08_425864 260 101 417 417 CD 10_1101-2021_01_08_425864 260 102 controlling control VBG 10_1101-2021_01_08_425864 260 103 the the DT 10_1101-2021_01_08_425864 260 104 entrance entrance NN 10_1101-2021_01_08_425864 260 105 of of IN 10_1101-2021_01_08_425864 260 106 long long JJ 10_1101-2021_01_08_425864 260 107 - - HYPH 10_1101-2021_01_08_425864 260 108 chain chain NN 10_1101-2021_01_08_425864 260 109 fatty fatty NN 10_1101-2021_01_08_425864 260 110 acids acid NNS 10_1101-2021_01_08_425864 260 111 into into IN 10_1101-2021_01_08_425864 260 112 the the DT 10_1101-2021_01_08_425864 260 113 mitochondria mitochondria NN 10_1101-2021_01_08_425864 260 114 [ [ -LRB- 10_1101-2021_01_08_425864 260 115 30 30 CD 10_1101-2021_01_08_425864 260 116 ] ] -RRB- 10_1101-2021_01_08_425864 260 117 . . . 10_1101-2021_01_08_425864 261 1 For for IN 10_1101-2021_01_08_425864 261 2 the the DT 10_1101-2021_01_08_425864 261 3 control control NN 10_1101-2021_01_08_425864 261 4 , , , 10_1101-2021_01_08_425864 261 5 we -PRON- PRP 10_1101-2021_01_08_425864 261 6 418 418 CD 10_1101-2021_01_08_425864 261 7 selected select VBD 10_1101-2021_01_08_425864 261 8 the the DT 10_1101-2021_01_08_425864 261 9 enzyme enzyme NNS 10_1101-2021_01_08_425864 261 10 serine serine NNP 10_1101-2021_01_08_425864 261 11 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 261 12 1 1 CD 10_1101-2021_01_08_425864 261 13 ( ( -LRB- 10_1101-2021_01_08_425864 261 14 SPT1 SPT1 NNP 10_1101-2021_01_08_425864 261 15 ) ) -RRB- 10_1101-2021_01_08_425864 261 16 , , , 10_1101-2021_01_08_425864 261 17 a a DT 10_1101-2021_01_08_425864 261 18 constitutively constitutively RB 10_1101-2021_01_08_425864 261 19 expressed express VBN 10_1101-2021_01_08_425864 261 20 protein protein NN 10_1101-2021_01_08_425864 261 21 in in IN 10_1101-2021_01_08_425864 261 22 T. T. NNP 10_1101-2021_01_08_425864 261 23 419 419 CD 10_1101-2021_01_08_425864 261 24 cruzi cruzi NNS 10_1101-2021_01_08_425864 261 25 [ [ -LRB- 10_1101-2021_01_08_425864 261 26 31 31 CD 10_1101-2021_01_08_425864 261 27 ] ] -RRB- 10_1101-2021_01_08_425864 261 28 ( ( -LRB- 10_1101-2021_01_08_425864 261 29 Fig fig NN 10_1101-2021_01_08_425864 261 30 7 7 CD 10_1101-2021_01_08_425864 261 31 ) ) -RRB- 10_1101-2021_01_08_425864 261 32 . . . 10_1101-2021_01_08_425864 262 1 The the DT 10_1101-2021_01_08_425864 262 2 hexokinase hexokinase NN 10_1101-2021_01_08_425864 262 3 activity activity NN 10_1101-2021_01_08_425864 262 4 diminished diminish VBN 10_1101-2021_01_08_425864 262 5 up up IN 10_1101-2021_01_08_425864 262 6 to to TO 10_1101-2021_01_08_425864 262 7 30 30 CD 10_1101-2021_01_08_425864 262 8 % % NN 10_1101-2021_01_08_425864 262 9 with with IN 10_1101-2021_01_08_425864 262 10 the the DT 10_1101-2021_01_08_425864 262 11 progression progression NN 10_1101-2021_01_08_425864 262 12 of of IN 10_1101-2021_01_08_425864 262 13 the the DT 10_1101-2021_01_08_425864 262 14 420 420 CD 10_1101-2021_01_08_425864 262 15 proliferation proliferation NN 10_1101-2021_01_08_425864 262 16 curve curve NN 10_1101-2021_01_08_425864 262 17 and and CC 10_1101-2021_01_08_425864 262 18 the the DT 10_1101-2021_01_08_425864 262 19 correlated correlate VBN 10_1101-2021_01_08_425864 262 20 depletion depletion NN 10_1101-2021_01_08_425864 262 21 of of IN 10_1101-2021_01_08_425864 262 22 glucose glucose NN 10_1101-2021_01_08_425864 262 23 ( ( -LRB- 10_1101-2021_01_08_425864 262 24 Fig fig NN 10_1101-2021_01_08_425864 262 25 7A 7a NN 10_1101-2021_01_08_425864 262 26 ) ) -RRB- 10_1101-2021_01_08_425864 262 27 . . . 10_1101-2021_01_08_425864 263 1 In in IN 10_1101-2021_01_08_425864 263 2 addition addition NN 10_1101-2021_01_08_425864 263 3 , , , 10_1101-2021_01_08_425864 263 4 the the DT 10_1101-2021_01_08_425864 263 5 ACC ACC NNP 10_1101-2021_01_08_425864 263 6 activity activity NN 10_1101-2021_01_08_425864 263 7 is be VBZ 10_1101-2021_01_08_425864 263 8 421 421 CD 10_1101-2021_01_08_425864 263 9 no no DT 10_1101-2021_01_08_425864 263 10 more more RBR 10_1101-2021_01_08_425864 263 11 detectable detectable JJ 10_1101-2021_01_08_425864 263 12 in in IN 10_1101-2021_01_08_425864 263 13 the the DT 10_1101-2021_01_08_425864 263 14 stationary stationary JJ 10_1101-2021_01_08_425864 263 15 phase phase NN 10_1101-2021_01_08_425864 263 16 cells cell NNS 10_1101-2021_01_08_425864 263 17 ( ( -LRB- 10_1101-2021_01_08_425864 263 18 Fig fig NN 10_1101-2021_01_08_425864 263 19 7B 7b NN 10_1101-2021_01_08_425864 263 20 ) ) -RRB- 10_1101-2021_01_08_425864 263 21 . . . 10_1101-2021_01_08_425864 264 1 By by IN 10_1101-2021_01_08_425864 264 2 contrast contrast NN 10_1101-2021_01_08_425864 264 3 , , , 10_1101-2021_01_08_425864 264 4 the the DT 10_1101-2021_01_08_425864 264 5 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 264 6 activity activity NN 10_1101-2021_01_08_425864 264 7 is be VBZ 10_1101-2021_01_08_425864 264 8 increased increase VBN 10_1101-2021_01_08_425864 264 9 422 422 CD 10_1101-2021_01_08_425864 264 10 by by IN 10_1101-2021_01_08_425864 264 11 ~4-fold ~4-fold NFP 10_1101-2021_01_08_425864 264 12 when when WRB 10_1101-2021_01_08_425864 264 13 the the DT 10_1101-2021_01_08_425864 264 14 stationary stationary JJ 10_1101-2021_01_08_425864 264 15 phase phase NN 10_1101-2021_01_08_425864 264 16 is be VBZ 10_1101-2021_01_08_425864 264 17 reached reach VBN 10_1101-2021_01_08_425864 264 18 ( ( -LRB- 10_1101-2021_01_08_425864 264 19 Fig Fig NNP 10_1101-2021_01_08_425864 264 20 7C 7C NNP 10_1101-2021_01_08_425864 264 21 ) ) -RRB- 10_1101-2021_01_08_425864 264 22 , , , 10_1101-2021_01_08_425864 264 23 which which WDT 10_1101-2021_01_08_425864 264 24 confirms confirm VBZ 10_1101-2021_01_08_425864 264 25 that that IN 10_1101-2021_01_08_425864 264 26 fatty fatty NN 10_1101-2021_01_08_425864 264 27 acid acid VBP 10_1101-2021_01_08_425864 264 28 degradation degradation NN 10_1101-2021_01_08_425864 264 29 423 423 CD 10_1101-2021_01_08_425864 264 30 occurs occur NNS 10_1101-2021_01_08_425864 264 31 in in IN 10_1101-2021_01_08_425864 264 32 the the DT 10_1101-2021_01_08_425864 264 33 absence absence NN 10_1101-2021_01_08_425864 264 34 of of IN 10_1101-2021_01_08_425864 264 35 glucose glucose NN 10_1101-2021_01_08_425864 264 36 . . . 10_1101-2021_01_08_425864 265 1 It -PRON- PRP 10_1101-2021_01_08_425864 265 2 is be VBZ 10_1101-2021_01_08_425864 265 3 noteworthy noteworthy JJ 10_1101-2021_01_08_425864 265 4 that that IN 10_1101-2021_01_08_425864 265 5 the the DT 10_1101-2021_01_08_425864 265 6 high high JJ 10_1101-2021_01_08_425864 265 7 levels level NNS 10_1101-2021_01_08_425864 265 8 of of IN 10_1101-2021_01_08_425864 265 9 ACC ACC NNP 10_1101-2021_01_08_425864 265 10 activity activity NN 10_1101-2021_01_08_425864 265 11 in in IN 10_1101-2021_01_08_425864 265 12 the the DT 10_1101-2021_01_08_425864 265 13 presence presence NN 10_1101-2021_01_08_425864 265 14 424 424 CD 10_1101-2021_01_08_425864 265 15 of of IN 10_1101-2021_01_08_425864 265 16 glucose glucose NN 10_1101-2021_01_08_425864 265 17 supports support VBZ 10_1101-2021_01_08_425864 265 18 the the DT 10_1101-2021_01_08_425864 265 19 idea idea NN 10_1101-2021_01_08_425864 265 20 that that IN 10_1101-2021_01_08_425864 265 21 under under IN 10_1101-2021_01_08_425864 265 22 these these DT 10_1101-2021_01_08_425864 265 23 conditions condition NNS 10_1101-2021_01_08_425864 265 24 , , , 10_1101-2021_01_08_425864 265 25 fatty fatty NN 10_1101-2021_01_08_425864 265 26 acids acid NNS 10_1101-2021_01_08_425864 265 27 are be VBP 10_1101-2021_01_08_425864 265 28 probably probably RB 10_1101-2021_01_08_425864 265 29 synthesized synthesize VBN 10_1101-2021_01_08_425864 265 30 instead instead RB 10_1101-2021_01_08_425864 265 31 425 425 CD 10_1101-2021_01_08_425864 265 32 of of IN 10_1101-2021_01_08_425864 265 33 being be VBG 10_1101-2021_01_08_425864 265 34 catabolized catabolize VBN 10_1101-2021_01_08_425864 265 35 . . . 10_1101-2021_01_08_425864 266 1 As as IN 10_1101-2021_01_08_425864 266 2 expected expect VBN 10_1101-2021_01_08_425864 266 3 , , , 10_1101-2021_01_08_425864 266 4 SPT1 SPT1 NNP 10_1101-2021_01_08_425864 266 5 did do VBD 10_1101-2021_01_08_425864 266 6 not not RB 10_1101-2021_01_08_425864 266 7 change change VB 10_1101-2021_01_08_425864 266 8 during during IN 10_1101-2021_01_08_425864 266 9 the the DT 10_1101-2021_01_08_425864 266 10 analysed analyse VBN 10_1101-2021_01_08_425864 266 11 time time NN 10_1101-2021_01_08_425864 266 12 frame frame NN 10_1101-2021_01_08_425864 266 13 ( ( -LRB- 10_1101-2021_01_08_425864 266 14 Fig fig NN 10_1101-2021_01_08_425864 266 15 7D 7D NNP 10_1101-2021_01_08_425864 266 16 ) ) -RRB- 10_1101-2021_01_08_425864 266 17 . . . 10_1101-2021_01_08_425864 267 1 426 426 CD 10_1101-2021_01_08_425864 267 2 427 427 CD 10_1101-2021_01_08_425864 267 3 Figure figure NN 10_1101-2021_01_08_425864 267 4 6 6 CD 10_1101-2021_01_08_425864 267 5 . . . 10_1101-2021_01_08_425864 268 1 Activities activity NNS 10_1101-2021_01_08_425864 268 2 of of IN 10_1101-2021_01_08_425864 268 3 enzymes enzyme NNS 10_1101-2021_01_08_425864 268 4 related relate VBN 10_1101-2021_01_08_425864 268 5 to to IN 10_1101-2021_01_08_425864 268 6 lipid lipid NN 10_1101-2021_01_08_425864 268 7 and and CC 10_1101-2021_01_08_425864 268 8 glucose glucose VB 10_1101-2021_01_08_425864 268 9 metabolism metabolism NN 10_1101-2021_01_08_425864 268 10 during during IN 10_1101-2021_01_08_425864 268 11 T. t. NN 10_1101-2021_01_08_425864 268 12 cruzi cruzi NNS 10_1101-2021_01_08_425864 268 13 growth growth NN 10_1101-2021_01_08_425864 268 14 428 428 CD 10_1101-2021_01_08_425864 268 15 curves curve NNS 10_1101-2021_01_08_425864 268 16 . . . 10_1101-2021_01_08_425864 269 1 A a LS 10_1101-2021_01_08_425864 269 2 ) ) -RRB- 10_1101-2021_01_08_425864 269 3 ( ( -LRB- 10_1101-2021_01_08_425864 269 4 HK HK NNP 10_1101-2021_01_08_425864 269 5 ) ) -RRB- 10_1101-2021_01_08_425864 269 6 Hexokinase Hexokinase NNP 10_1101-2021_01_08_425864 269 7 B B NNP 10_1101-2021_01_08_425864 269 8 ) ) -RRB- 10_1101-2021_01_08_425864 269 9 ( ( -LRB- 10_1101-2021_01_08_425864 269 10 ACC ACC NNP 10_1101-2021_01_08_425864 269 11 ) ) -RRB- 10_1101-2021_01_08_425864 269 12 acetyl acetyl NN 10_1101-2021_01_08_425864 269 13 - - HYPH 10_1101-2021_01_08_425864 269 14 CoA CoA NNP 10_1101-2021_01_08_425864 269 15 carboxylase carboxylase NN 10_1101-2021_01_08_425864 269 16 , , , 10_1101-2021_01_08_425864 269 17 C C NNP 10_1101-2021_01_08_425864 269 18 ) ) -RRB- 10_1101-2021_01_08_425864 269 19 ( ( -LRB- 10_1101-2021_01_08_425864 269 20 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 269 21 ) ) -RRB- 10_1101-2021_01_08_425864 269 22 carnitine- carnitine- NN 10_1101-2021_01_08_425864 269 23 429 429 CD 10_1101-2021_01_08_425864 269 24 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 269 25 , , , 10_1101-2021_01_08_425864 269 26 and and CC 10_1101-2021_01_08_425864 269 27 D d NN 10_1101-2021_01_08_425864 269 28 ) ) -RRB- 10_1101-2021_01_08_425864 269 29 ( ( -LRB- 10_1101-2021_01_08_425864 269 30 SPT SPT NNP 10_1101-2021_01_08_425864 269 31 ) ) -RRB- 10_1101-2021_01_08_425864 269 32 serine serine JJ 10_1101-2021_01_08_425864 269 33 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 269 34 . . . 10_1101-2021_01_08_425864 270 1 All all PDT 10_1101-2021_01_08_425864 270 2 these these DT 10_1101-2021_01_08_425864 270 3 activities activity NNS 10_1101-2021_01_08_425864 270 4 were be VBD 10_1101-2021_01_08_425864 270 5 measured measure VBN 10_1101-2021_01_08_425864 270 6 in in IN 10_1101-2021_01_08_425864 270 7 .CC .CC NFP 10_1101-2021_01_08_425864 270 8 - - : 10_1101-2021_01_08_425864 270 9 BY by IN 10_1101-2021_01_08_425864 270 10 4.0 4.0 CD 10_1101-2021_01_08_425864 270 11 International international JJ 10_1101-2021_01_08_425864 270 12 licensemade licensemade NN 10_1101-2021_01_08_425864 270 13 available available JJ 10_1101-2021_01_08_425864 270 14 under under IN 10_1101-2021_01_08_425864 270 15 a a DT 10_1101-2021_01_08_425864 270 16 ( ( -LRB- 10_1101-2021_01_08_425864 270 17 which which WDT 10_1101-2021_01_08_425864 270 18 was be VBD 10_1101-2021_01_08_425864 270 19 not not RB 10_1101-2021_01_08_425864 270 20 certified certify VBN 10_1101-2021_01_08_425864 270 21 by by IN 10_1101-2021_01_08_425864 270 22 peer peer NN 10_1101-2021_01_08_425864 270 23 review review NN 10_1101-2021_01_08_425864 270 24 ) ) -RRB- 10_1101-2021_01_08_425864 270 25 is be VBZ 10_1101-2021_01_08_425864 270 26 the the DT 10_1101-2021_01_08_425864 270 27 author author NN 10_1101-2021_01_08_425864 270 28 / / SYM 10_1101-2021_01_08_425864 270 29 funder funder NN 10_1101-2021_01_08_425864 270 30 , , , 10_1101-2021_01_08_425864 270 31 who who WP 10_1101-2021_01_08_425864 270 32 has have VBZ 10_1101-2021_01_08_425864 270 33 granted grant VBN 10_1101-2021_01_08_425864 270 34 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 270 35 a a DT 10_1101-2021_01_08_425864 270 36 license license NN 10_1101-2021_01_08_425864 270 37 to to TO 10_1101-2021_01_08_425864 270 38 display display VB 10_1101-2021_01_08_425864 270 39 the the DT 10_1101-2021_01_08_425864 270 40 preprint preprint NN 10_1101-2021_01_08_425864 270 41 in in IN 10_1101-2021_01_08_425864 270 42 perpetuity perpetuity NN 10_1101-2021_01_08_425864 270 43 . . . 10_1101-2021_01_08_425864 271 1 It -PRON- PRP 10_1101-2021_01_08_425864 271 2 is be VBZ 10_1101-2021_01_08_425864 271 3 The the DT 10_1101-2021_01_08_425864 271 4 copyright copyright NN 10_1101-2021_01_08_425864 271 5 holder holder NN 10_1101-2021_01_08_425864 271 6 for for IN 10_1101-2021_01_08_425864 271 7 this this DT 10_1101-2021_01_08_425864 271 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 271 9 version version NN 10_1101-2021_01_08_425864 271 10 posted post VBD 10_1101-2021_01_08_425864 271 11 January January NNP 10_1101-2021_01_08_425864 271 12 8 8 CD 10_1101-2021_01_08_425864 271 13 , , , 10_1101-2021_01_08_425864 271 14 2021 2021 CD 10_1101-2021_01_08_425864 271 15 . . . 10_1101-2021_01_08_425864 271 16 ; ; : 10_1101-2021_01_08_425864 271 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 271 18 : : : 10_1101-2021_01_08_425864 271 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 271 20 preprint preprint NN 10_1101-2021_01_08_425864 271 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 271 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ VBZ 10_1101-2021_01_08_425864 271 23 430 430 CD 10_1101-2021_01_08_425864 271 24 crude crude JJ 10_1101-2021_01_08_425864 271 25 extracts extract NNS 10_1101-2021_01_08_425864 271 26 from from IN 10_1101-2021_01_08_425864 271 27 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 271 28 forms form NNS 10_1101-2021_01_08_425864 271 29 at at IN 10_1101-2021_01_08_425864 271 30 different different JJ 10_1101-2021_01_08_425864 271 31 moments moment NNS 10_1101-2021_01_08_425864 271 32 of of IN 10_1101-2021_01_08_425864 271 33 the the DT 10_1101-2021_01_08_425864 271 34 growth growth NN 10_1101-2021_01_08_425864 271 35 curve curve NN 10_1101-2021_01_08_425864 271 36 . . . 10_1101-2021_01_08_425864 272 1 All all PDT 10_1101-2021_01_08_425864 272 2 the the DT 10_1101-2021_01_08_425864 272 3 experiments experiment NNS 10_1101-2021_01_08_425864 272 4 431 431 CD 10_1101-2021_01_08_425864 272 5 were be VBD 10_1101-2021_01_08_425864 272 6 performed perform VBN 10_1101-2021_01_08_425864 272 7 in in IN 10_1101-2021_01_08_425864 272 8 triplicates triplicate NNS 10_1101-2021_01_08_425864 272 9 . . . 10_1101-2021_01_08_425864 273 1 Time time NN 10_1101-2021_01_08_425864 273 2 course course NN 10_1101-2021_01_08_425864 273 3 activities activity NNS 10_1101-2021_01_08_425864 273 4 and and CC 10_1101-2021_01_08_425864 273 5 controls control NNS 10_1101-2021_01_08_425864 273 6 shown show VBN 10_1101-2021_01_08_425864 273 7 in in IN 10_1101-2021_01_08_425864 273 8 Fig Fig NNP 10_1101-2021_01_08_425864 273 9 S2 S2 NNP 10_1101-2021_01_08_425864 273 10 . . . 10_1101-2021_01_08_425864 274 1 Statistical statistical JJ 10_1101-2021_01_08_425864 274 2 analysis analysis NN 10_1101-2021_01_08_425864 274 3 432 432 CD 10_1101-2021_01_08_425864 274 4 was be VBD 10_1101-2021_01_08_425864 274 5 performed perform VBN 10_1101-2021_01_08_425864 274 6 with with IN 10_1101-2021_01_08_425864 274 7 one one CD 10_1101-2021_01_08_425864 274 8 - - HYPH 10_1101-2021_01_08_425864 274 9 way way NN 10_1101-2021_01_08_425864 274 10 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 274 11 followed follow VBN 10_1101-2021_01_08_425864 274 12 by by IN 10_1101-2021_01_08_425864 274 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 274 14 's 's POS 10_1101-2021_01_08_425864 274 15 post post JJ 10_1101-2021_01_08_425864 274 16 - - NN 10_1101-2021_01_08_425864 274 17 test test NN 10_1101-2021_01_08_425864 274 18 at at IN 10_1101-2021_01_08_425864 274 19 p p NN 10_1101-2021_01_08_425864 274 20 < < XX 10_1101-2021_01_08_425864 274 21 0.05 0.05 CD 10_1101-2021_01_08_425864 274 22 , , , 10_1101-2021_01_08_425864 274 23 using use VBG 10_1101-2021_01_08_425864 274 24 the the DT 10_1101-2021_01_08_425864 274 25 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 274 26 433 433 CD 10_1101-2021_01_08_425864 274 27 Prism Prism NNP 10_1101-2021_01_08_425864 274 28 8.0.2 8.0.2 CD 10_1101-2021_01_08_425864 274 29 software software NN 10_1101-2021_01_08_425864 274 30 program program NN 10_1101-2021_01_08_425864 274 31 . . . 10_1101-2021_01_08_425864 275 1 We -PRON- PRP 10_1101-2021_01_08_425864 275 2 represent represent VBP 10_1101-2021_01_08_425864 275 3 the the DT 10_1101-2021_01_08_425864 275 4 level level NN 10_1101-2021_01_08_425864 275 5 of of IN 10_1101-2021_01_08_425864 275 6 statistical statistical JJ 10_1101-2021_01_08_425864 275 7 significance significance NN 10_1101-2021_01_08_425864 275 8 in in IN 10_1101-2021_01_08_425864 275 9 this this DT 10_1101-2021_01_08_425864 275 10 figure figure NN 10_1101-2021_01_08_425864 275 11 as as IN 10_1101-2021_01_08_425864 275 12 434 434 CD 10_1101-2021_01_08_425864 275 13 follows follow VBZ 10_1101-2021_01_08_425864 275 14 : : : 10_1101-2021_01_08_425864 275 15 * * NFP 10_1101-2021_01_08_425864 275 16 * * NFP 10_1101-2021_01_08_425864 275 17 * * NFP 10_1101-2021_01_08_425864 275 18 p p NN 10_1101-2021_01_08_425864 275 19 value value NN 10_1101-2021_01_08_425864 275 20 < < XX 10_1101-2021_01_08_425864 275 21 0.001 0.001 CD 10_1101-2021_01_08_425864 275 22 ; ; : 10_1101-2021_01_08_425864 275 23 * * NFP 10_1101-2021_01_08_425864 275 24 * * NFP 10_1101-2021_01_08_425864 275 25 p p NN 10_1101-2021_01_08_425864 275 26 value value NN 10_1101-2021_01_08_425864 275 27 < < XX 10_1101-2021_01_08_425864 275 28 0.01 0.01 XX 10_1101-2021_01_08_425864 275 29 ; ; : 10_1101-2021_01_08_425864 275 30 and and CC 10_1101-2021_01_08_425864 275 31 * * NFP 10_1101-2021_01_08_425864 275 32 p p NN 10_1101-2021_01_08_425864 275 33 value value NN 10_1101-2021_01_08_425864 275 34 < < XX 10_1101-2021_01_08_425864 275 35 0.05 0.05 CD 10_1101-2021_01_08_425864 275 36 . . . 10_1101-2021_01_08_425864 276 1 For for IN 10_1101-2021_01_08_425864 276 2 p p NN 10_1101-2021_01_08_425864 276 3 value value NN 10_1101-2021_01_08_425864 276 4 > > XX 10_1101-2021_01_08_425864 276 5 0.05 0.05 CD 10_1101-2021_01_08_425864 276 6 we -PRON- PRP 10_1101-2021_01_08_425864 276 7 consider consider VBP 10_1101-2021_01_08_425864 276 8 435 435 CD 10_1101-2021_01_08_425864 276 9 the the DT 10_1101-2021_01_08_425864 276 10 differences difference NNS 10_1101-2021_01_08_425864 276 11 not not RB 10_1101-2021_01_08_425864 276 12 significant significant JJ 10_1101-2021_01_08_425864 276 13 ( ( -LRB- 10_1101-2021_01_08_425864 276 14 ns ns NNP 10_1101-2021_01_08_425864 276 15 ) ) -RRB- 10_1101-2021_01_08_425864 276 16 . . . 10_1101-2021_01_08_425864 277 1 436 436 CD 10_1101-2021_01_08_425864 277 2 437 437 CD 10_1101-2021_01_08_425864 277 3 438 438 CD 10_1101-2021_01_08_425864 277 4 Etomoxir Etomoxir NNPS 10_1101-2021_01_08_425864 277 5 , , , 10_1101-2021_01_08_425864 277 6 a a DT 10_1101-2021_01_08_425864 277 7 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 277 8 inhibitor inhibitor NN 10_1101-2021_01_08_425864 277 9 , , , 10_1101-2021_01_08_425864 277 10 affects affect VBZ 10_1101-2021_01_08_425864 277 11 T. T. NNP 10_1101-2021_01_08_425864 277 12 cruzi cruzi NNP 10_1101-2021_01_08_425864 277 13 proliferation proliferation NN 10_1101-2021_01_08_425864 277 14 and and CC 10_1101-2021_01_08_425864 277 15 mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 277 16 activity activity NN 10_1101-2021_01_08_425864 277 17 439 439 CD 10_1101-2021_01_08_425864 277 18 To to TO 10_1101-2021_01_08_425864 277 19 investigate investigate VB 10_1101-2021_01_08_425864 277 20 the the DT 10_1101-2021_01_08_425864 277 21 role role NN 10_1101-2021_01_08_425864 277 22 of of IN 10_1101-2021_01_08_425864 277 23 FAO FAO NNP 10_1101-2021_01_08_425864 277 24 in in IN 10_1101-2021_01_08_425864 277 25 T. T. NNP 10_1101-2021_01_08_425864 277 26 cruzi cruzi NNS 10_1101-2021_01_08_425864 277 27 , , , 10_1101-2021_01_08_425864 277 28 we -PRON- PRP 10_1101-2021_01_08_425864 277 29 tested test VBD 10_1101-2021_01_08_425864 277 30 the the DT 10_1101-2021_01_08_425864 277 31 effect effect NN 10_1101-2021_01_08_425864 277 32 of of IN 10_1101-2021_01_08_425864 277 33 a a DT 10_1101-2021_01_08_425864 277 34 well well RB 10_1101-2021_01_08_425864 277 35 characterized characterize VBN 10_1101-2021_01_08_425864 277 36 inhibitor inhibitor NN 10_1101-2021_01_08_425864 277 37 440 440 CD 10_1101-2021_01_08_425864 277 38 of of IN 10_1101-2021_01_08_425864 277 39 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 277 40 , , , 10_1101-2021_01_08_425864 277 41 etomoxir etomoxir NNP 10_1101-2021_01_08_425864 277 42 ( ( -LRB- 10_1101-2021_01_08_425864 277 43 ETO ETO NNP 10_1101-2021_01_08_425864 277 44 ) ) -RRB- 10_1101-2021_01_08_425864 277 45 , , , 10_1101-2021_01_08_425864 277 46 on on IN 10_1101-2021_01_08_425864 277 47 the the DT 10_1101-2021_01_08_425864 277 48 proliferation proliferation NN 10_1101-2021_01_08_425864 277 49 of of IN 10_1101-2021_01_08_425864 277 50 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 277 51 . . . 10_1101-2021_01_08_425864 278 1 Among among IN 10_1101-2021_01_08_425864 278 2 the the DT 10_1101-2021_01_08_425864 278 3 ETO ETO NNP 10_1101-2021_01_08_425864 278 4 concentrations concentration NNS 10_1101-2021_01_08_425864 278 5 441 441 CD 10_1101-2021_01_08_425864 278 6 tested test VBN 10_1101-2021_01_08_425864 278 7 here here RB 10_1101-2021_01_08_425864 278 8 ( ( -LRB- 10_1101-2021_01_08_425864 278 9 from from IN 10_1101-2021_01_08_425864 278 10 0.1 0.1 CD 10_1101-2021_01_08_425864 278 11 to to IN 10_1101-2021_01_08_425864 278 12 500 500 CD 10_1101-2021_01_08_425864 278 13 µM µM NNP 10_1101-2021_01_08_425864 278 14 ) ) -RRB- 10_1101-2021_01_08_425864 278 15 , , , 10_1101-2021_01_08_425864 278 16 only only RB 10_1101-2021_01_08_425864 278 17 the the DT 10_1101-2021_01_08_425864 278 18 higher high JJR 10_1101-2021_01_08_425864 278 19 concentration concentration NN 10_1101-2021_01_08_425864 278 20 arrested arrest VBN 10_1101-2021_01_08_425864 278 21 parasite parasite NN 10_1101-2021_01_08_425864 278 22 proliferation proliferation NN 10_1101-2021_01_08_425864 278 23 ( ( -LRB- 10_1101-2021_01_08_425864 278 24 Fig fig NN 10_1101-2021_01_08_425864 278 25 442 442 CD 10_1101-2021_01_08_425864 278 26 8A 8A NNP 10_1101-2021_01_08_425864 278 27 ) ) -RRB- 10_1101-2021_01_08_425864 278 28 . . . 10_1101-2021_01_08_425864 279 1 Importantly importantly RB 10_1101-2021_01_08_425864 279 2 , , , 10_1101-2021_01_08_425864 279 3 the the DT 10_1101-2021_01_08_425864 279 4 ETO ETO NNP 10_1101-2021_01_08_425864 279 5 effect effect NN 10_1101-2021_01_08_425864 279 6 was be VBD 10_1101-2021_01_08_425864 279 7 manifested manifest VBN 10_1101-2021_01_08_425864 279 8 when when WRB 10_1101-2021_01_08_425864 279 9 the the DT 10_1101-2021_01_08_425864 279 10 parasites parasite NNS 10_1101-2021_01_08_425864 279 11 reached reach VBD 10_1101-2021_01_08_425864 279 12 the the DT 10_1101-2021_01_08_425864 279 13 late late JJ 10_1101-2021_01_08_425864 279 14 exponential exponential JJ 10_1101-2021_01_08_425864 279 15 443 443 CD 10_1101-2021_01_08_425864 279 16 phase phase NN 10_1101-2021_01_08_425864 279 17 ( ( -LRB- 10_1101-2021_01_08_425864 279 18 a a DT 10_1101-2021_01_08_425864 279 19 cell cell NN 10_1101-2021_01_08_425864 279 20 density density NN 10_1101-2021_01_08_425864 279 21 of of IN 10_1101-2021_01_08_425864 279 22 approximately approximately RB 10_1101-2021_01_08_425864 279 23 5x107 5x107 CD 10_1101-2021_01_08_425864 279 24 mL-1 ml-1 NN 10_1101-2021_01_08_425864 279 25 ) ) -RRB- 10_1101-2021_01_08_425864 279 26 . . . 10_1101-2021_01_08_425864 280 1 This this DT 10_1101-2021_01_08_425864 280 2 result result NN 10_1101-2021_01_08_425864 280 3 is be VBZ 10_1101-2021_01_08_425864 280 4 consistent consistent JJ 10_1101-2021_01_08_425864 280 5 with with IN 10_1101-2021_01_08_425864 280 6 our -PRON- PRP$ 10_1101-2021_01_08_425864 280 7 previous previous JJ 10_1101-2021_01_08_425864 280 8 444 444 CD 10_1101-2021_01_08_425864 280 9 findings finding NNS 10_1101-2021_01_08_425864 280 10 showing show VBG 10_1101-2021_01_08_425864 280 11 that that IN 10_1101-2021_01_08_425864 280 12 FAO FAO NNP 10_1101-2021_01_08_425864 280 13 ( ( -LRB- 10_1101-2021_01_08_425864 280 14 and and CC 10_1101-2021_01_08_425864 280 15 thus thus RB 10_1101-2021_01_08_425864 280 16 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 280 17 activity activity NN 10_1101-2021_01_08_425864 280 18 ) ) -RRB- 10_1101-2021_01_08_425864 280 19 acquires acquire VBZ 10_1101-2021_01_08_425864 280 20 an an DT 10_1101-2021_01_08_425864 280 21 important important JJ 10_1101-2021_01_08_425864 280 22 role role NN 10_1101-2021_01_08_425864 280 23 at at IN 10_1101-2021_01_08_425864 280 24 this this DT 10_1101-2021_01_08_425864 280 25 point point NN 10_1101-2021_01_08_425864 280 26 in in IN 10_1101-2021_01_08_425864 280 27 the the DT 10_1101-2021_01_08_425864 280 28 445 445 CD 10_1101-2021_01_08_425864 280 29 proliferation proliferation NN 10_1101-2021_01_08_425864 280 30 curve curve NN 10_1101-2021_01_08_425864 280 31 . . . 10_1101-2021_01_08_425864 281 1 To to TO 10_1101-2021_01_08_425864 281 2 confirm confirm VB 10_1101-2021_01_08_425864 281 3 that that IN 10_1101-2021_01_08_425864 281 4 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 281 5 is be VBZ 10_1101-2021_01_08_425864 281 6 in in IN 10_1101-2021_01_08_425864 281 7 fact fact NN 10_1101-2021_01_08_425864 281 8 a a DT 10_1101-2021_01_08_425864 281 9 target target NN 10_1101-2021_01_08_425864 281 10 of of IN 10_1101-2021_01_08_425864 281 11 ETO eto NN 10_1101-2021_01_08_425864 281 12 in in IN 10_1101-2021_01_08_425864 281 13 T. T. NNP 10_1101-2021_01_08_425864 281 14 cruzi cruzi NNS 10_1101-2021_01_08_425864 281 15 , , , 10_1101-2021_01_08_425864 281 16 we -PRON- PRP 10_1101-2021_01_08_425864 281 17 assayed assay VBD 10_1101-2021_01_08_425864 281 18 the the DT 10_1101-2021_01_08_425864 281 19 drug drug NN 10_1101-2021_01_08_425864 281 20 's 's POS 10_1101-2021_01_08_425864 281 21 446 446 CD 10_1101-2021_01_08_425864 281 22 effect effect NN 10_1101-2021_01_08_425864 281 23 on on IN 10_1101-2021_01_08_425864 281 24 the the DT 10_1101-2021_01_08_425864 281 25 enzyme enzyme NNS 10_1101-2021_01_08_425864 281 26 activity activity NN 10_1101-2021_01_08_425864 281 27 in in IN 10_1101-2021_01_08_425864 281 28 free free JJ 10_1101-2021_01_08_425864 281 29 cell cell NN 10_1101-2021_01_08_425864 281 30 extracts extract NNS 10_1101-2021_01_08_425864 281 31 . . . 10_1101-2021_01_08_425864 282 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 282 2 results result NNS 10_1101-2021_01_08_425864 282 3 showed show VBD 10_1101-2021_01_08_425864 282 4 that that IN 10_1101-2021_01_08_425864 282 5 500 500 CD 10_1101-2021_01_08_425864 282 6 µM µM NNP 10_1101-2021_01_08_425864 282 7 ETO ETO NNP 10_1101-2021_01_08_425864 282 8 diminished diminish VBD 10_1101-2021_01_08_425864 282 9 447 447 CD 10_1101-2021_01_08_425864 282 10 the the DT 10_1101-2021_01_08_425864 282 11 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 282 12 activity activity NN 10_1101-2021_01_08_425864 282 13 by by IN 10_1101-2021_01_08_425864 282 14 almost almost RB 10_1101-2021_01_08_425864 282 15 80 80 CD 10_1101-2021_01_08_425864 282 16 % % NN 10_1101-2021_01_08_425864 282 17 ( ( -LRB- 10_1101-2021_01_08_425864 282 18 Fig fig NN 10_1101-2021_01_08_425864 282 19 8B 8b NN 10_1101-2021_01_08_425864 282 20 ) ) -RRB- 10_1101-2021_01_08_425864 282 21 . . . 10_1101-2021_01_08_425864 283 1 To to TO 10_1101-2021_01_08_425864 283 2 confirm confirm VB 10_1101-2021_01_08_425864 283 3 the the DT 10_1101-2021_01_08_425864 283 4 interference interference NN 10_1101-2021_01_08_425864 283 5 of of IN 10_1101-2021_01_08_425864 283 6 ETO ETO NNP 10_1101-2021_01_08_425864 283 7 with with IN 10_1101-2021_01_08_425864 283 8 the the DT 10_1101-2021_01_08_425864 283 9 beta- beta- JJ 10_1101-2021_01_08_425864 283 10 448 448 CD 10_1101-2021_01_08_425864 283 11 oxidation oxidation NN 10_1101-2021_01_08_425864 283 12 of of IN 10_1101-2021_01_08_425864 283 13 fatty fatty NN 10_1101-2021_01_08_425864 283 14 acids acid NNS 10_1101-2021_01_08_425864 283 15 , , , 10_1101-2021_01_08_425864 283 16 parasites parasite NNS 10_1101-2021_01_08_425864 283 17 incubated incubate VBD 10_1101-2021_01_08_425864 283 18 in in IN 10_1101-2021_01_08_425864 283 19 PBS PBS NNP 10_1101-2021_01_08_425864 283 20 containing contain VBG 10_1101-2021_01_08_425864 283 21 14C 14c CD 10_1101-2021_01_08_425864 283 22 - - HYPH 10_1101-2021_01_08_425864 283 23 U u NN 10_1101-2021_01_08_425864 283 24 - - HYPH 10_1101-2021_01_08_425864 283 25 palmitate palmitate NN 10_1101-2021_01_08_425864 283 26 were be VBD 10_1101-2021_01_08_425864 283 27 treated treat VBN 10_1101-2021_01_08_425864 283 28 with with IN 10_1101-2021_01_08_425864 283 29 500 500 CD 10_1101-2021_01_08_425864 283 30 449 449 CD 10_1101-2021_01_08_425864 283 31 µM µM NNP 10_1101-2021_01_08_425864 283 32 ETO eto NN 10_1101-2021_01_08_425864 283 33 to to TO 10_1101-2021_01_08_425864 283 34 compare compare VB 10_1101-2021_01_08_425864 283 35 their -PRON- PRP$ 10_1101-2021_01_08_425864 283 36 production production NN 10_1101-2021_01_08_425864 283 37 of of IN 10_1101-2021_01_08_425864 283 38 14CO2 14co2 CD 10_1101-2021_01_08_425864 283 39 with with IN 10_1101-2021_01_08_425864 283 40 that that DT 10_1101-2021_01_08_425864 283 41 of of IN 10_1101-2021_01_08_425864 283 42 the the DT 10_1101-2021_01_08_425864 283 43 untreated untreated JJ 10_1101-2021_01_08_425864 283 44 controls control NNS 10_1101-2021_01_08_425864 283 45 . . . 10_1101-2021_01_08_425864 284 1 Palmitate Palmitate NNP 10_1101-2021_01_08_425864 284 2 - - HYPH 10_1101-2021_01_08_425864 284 3 derived derive VBN 10_1101-2021_01_08_425864 284 4 450 450 CD 10_1101-2021_01_08_425864 284 5 CO2 CO2 NNP 10_1101-2021_01_08_425864 284 6 production production NN 10_1101-2021_01_08_425864 284 7 diminished diminish VBN 10_1101-2021_01_08_425864 284 8 by by IN 10_1101-2021_01_08_425864 284 9 80 80 CD 10_1101-2021_01_08_425864 284 10 % % NN 10_1101-2021_01_08_425864 284 11 in in IN 10_1101-2021_01_08_425864 284 12 ETO ETO NNP 10_1101-2021_01_08_425864 284 13 - - HYPH 10_1101-2021_01_08_425864 284 14 treated treat VBN 10_1101-2021_01_08_425864 284 15 cells cell NNS 10_1101-2021_01_08_425864 284 16 compared compare VBN 10_1101-2021_01_08_425864 284 17 to to IN 10_1101-2021_01_08_425864 284 18 untreated untreated JJ 10_1101-2021_01_08_425864 284 19 parasites parasite NNS 10_1101-2021_01_08_425864 284 20 ( ( -LRB- 10_1101-2021_01_08_425864 284 21 Fig fig NN 10_1101-2021_01_08_425864 284 22 8C 8c NN 10_1101-2021_01_08_425864 284 23 ) ) -RRB- 10_1101-2021_01_08_425864 284 24 . . . 10_1101-2021_01_08_425864 285 1 451 451 CD 10_1101-2021_01_08_425864 285 2 In in IN 10_1101-2021_01_08_425864 285 3 addition addition NN 10_1101-2021_01_08_425864 285 4 , , , 10_1101-2021_01_08_425864 285 5 ETO ETO NNP 10_1101-2021_01_08_425864 285 6 treatment treatment NN 10_1101-2021_01_08_425864 285 7 did do VBD 10_1101-2021_01_08_425864 285 8 not not RB 10_1101-2021_01_08_425864 285 9 affect affect VB 10_1101-2021_01_08_425864 285 10 the the DT 10_1101-2021_01_08_425864 285 11 metabolism metabolism NN 10_1101-2021_01_08_425864 285 12 of of IN 10_1101-2021_01_08_425864 285 13 14C 14c CD 10_1101-2021_01_08_425864 285 14 - - HYPH 10_1101-2021_01_08_425864 285 15 U u NN 10_1101-2021_01_08_425864 285 16 - - HYPH 10_1101-2021_01_08_425864 285 17 glucose glucose NN 10_1101-2021_01_08_425864 285 18 or or CC 10_1101-2021_01_08_425864 285 19 14C 14c NN 10_1101-2021_01_08_425864 285 20 - - HYPH 10_1101-2021_01_08_425864 285 21 U u NN 10_1101-2021_01_08_425864 285 22 - - HYPH 10_1101-2021_01_08_425864 285 23 histidine histidine NN 10_1101-2021_01_08_425864 285 24 , , , 10_1101-2021_01_08_425864 285 25 ruling rule VBG 10_1101-2021_01_08_425864 285 26 452 452 CD 10_1101-2021_01_08_425864 285 27 out out RP 10_1101-2021_01_08_425864 285 28 a a DT 10_1101-2021_01_08_425864 285 29 possible possible JJ 10_1101-2021_01_08_425864 285 30 unspecific unspecific JJ 10_1101-2021_01_08_425864 285 31 reaction reaction NN 10_1101-2021_01_08_425864 285 32 of of IN 10_1101-2021_01_08_425864 285 33 this this DT 10_1101-2021_01_08_425864 285 34 drug drug NN 10_1101-2021_01_08_425864 285 35 with with IN 10_1101-2021_01_08_425864 285 36 CoA CoA NNP 10_1101-2021_01_08_425864 285 37 - - HYPH 10_1101-2021_01_08_425864 285 38 SH SH NNP 10_1101-2021_01_08_425864 285 39 as as IN 10_1101-2021_01_08_425864 285 40 described describe VBN 10_1101-2021_01_08_425864 285 41 by by IN 10_1101-2021_01_08_425864 285 42 [ [ -LRB- 10_1101-2021_01_08_425864 285 43 32 32 CD 10_1101-2021_01_08_425864 285 44 ] ] -RRB- 10_1101-2021_01_08_425864 285 45 . . . 10_1101-2021_01_08_425864 286 1 Other other JJ 10_1101-2021_01_08_425864 286 2 compounds compound NNS 10_1101-2021_01_08_425864 286 3 453 453 CD 10_1101-2021_01_08_425864 286 4 described describe VBN 10_1101-2021_01_08_425864 286 5 as as IN 10_1101-2021_01_08_425864 286 6 FAO FAO NNP 10_1101-2021_01_08_425864 286 7 inhibitors inhibitor NNS 10_1101-2021_01_08_425864 286 8 were be VBD 10_1101-2021_01_08_425864 286 9 also also RB 10_1101-2021_01_08_425864 286 10 tested test VBN 10_1101-2021_01_08_425864 286 11 , , , 10_1101-2021_01_08_425864 286 12 but but CC 10_1101-2021_01_08_425864 286 13 none none NN 10_1101-2021_01_08_425864 286 14 of of IN 10_1101-2021_01_08_425864 286 15 them -PRON- PRP 10_1101-2021_01_08_425864 286 16 inhibited inhibit VBD 10_1101-2021_01_08_425864 286 17 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 286 18 proliferation proliferation NN 10_1101-2021_01_08_425864 286 19 454 454 CD 10_1101-2021_01_08_425864 286 20 or or CC 10_1101-2021_01_08_425864 286 21 14CO2 14co2 CD 10_1101-2021_01_08_425864 286 22 production production NN 10_1101-2021_01_08_425864 286 23 from from IN 10_1101-2021_01_08_425864 286 24 14C 14c CD 10_1101-2021_01_08_425864 286 25 - - HYPH 10_1101-2021_01_08_425864 286 26 U u NN 10_1101-2021_01_08_425864 286 27 - - HYPH 10_1101-2021_01_08_425864 286 28 palmitate palmitate NN 10_1101-2021_01_08_425864 286 29 ( ( -LRB- 10_1101-2021_01_08_425864 286 30 S3 S3 NNP 10_1101-2021_01_08_425864 286 31 Fig Fig NNP 10_1101-2021_01_08_425864 286 32 ) ) -RRB- 10_1101-2021_01_08_425864 286 33 . . . 10_1101-2021_01_08_425864 287 1 In in IN 10_1101-2021_01_08_425864 287 2 addition addition NN 10_1101-2021_01_08_425864 287 3 , , , 10_1101-2021_01_08_425864 287 4 the the DT 10_1101-2021_01_08_425864 287 5 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 287 6 cytometric cytometric NN 10_1101-2021_01_08_425864 287 7 analysis analysis NN 10_1101-2021_01_08_425864 287 8 of of IN 10_1101-2021_01_08_425864 287 9 455 455 CD 10_1101-2021_01_08_425864 287 10 cells cell NNS 10_1101-2021_01_08_425864 287 11 treated treat VBN 10_1101-2021_01_08_425864 287 12 with with IN 10_1101-2021_01_08_425864 287 13 500 500 CD 10_1101-2021_01_08_425864 287 14 µM µM NNP 10_1101-2021_01_08_425864 287 15 ETO ETO NNP 10_1101-2021_01_08_425864 287 16 showed show VBD 10_1101-2021_01_08_425864 287 17 a a DT 10_1101-2021_01_08_425864 287 18 strong strong JJ 10_1101-2021_01_08_425864 287 19 decrease decrease NN 10_1101-2021_01_08_425864 287 20 in in IN 10_1101-2021_01_08_425864 287 21 the the DT 10_1101-2021_01_08_425864 287 22 CoA CoA NNP 10_1101-2021_01_08_425864 287 23 acylation acylation NN 10_1101-2021_01_08_425864 287 24 levels level NNS 10_1101-2021_01_08_425864 287 25 ( ( -LRB- 10_1101-2021_01_08_425864 287 26 activation activation NN 10_1101-2021_01_08_425864 287 27 of of IN 10_1101-2021_01_08_425864 287 28 456 456 CD 10_1101-2021_01_08_425864 287 29 fatty fatty NN 10_1101-2021_01_08_425864 287 30 acids acid NNS 10_1101-2021_01_08_425864 287 31 ) ) -RRB- 10_1101-2021_01_08_425864 287 32 with with IN 10_1101-2021_01_08_425864 287 33 respect respect NN 10_1101-2021_01_08_425864 287 34 to to IN 10_1101-2021_01_08_425864 287 35 the the DT 10_1101-2021_01_08_425864 287 36 untreated untreated JJ 10_1101-2021_01_08_425864 287 37 controls control NNS 10_1101-2021_01_08_425864 287 38 ( ( -LRB- 10_1101-2021_01_08_425864 287 39 Fig fig NN 10_1101-2021_01_08_425864 287 40 8D 8d NN 10_1101-2021_01_08_425864 287 41 ) ) -RRB- 10_1101-2021_01_08_425864 287 42 , , , 10_1101-2021_01_08_425864 287 43 as as IN 10_1101-2021_01_08_425864 287 44 confirmed confirm VBN 10_1101-2021_01_08_425864 287 45 by by IN 10_1101-2021_01_08_425864 287 46 fluorescence fluorescence NN 10_1101-2021_01_08_425864 287 47 microscopy microscopy JJ 10_1101-2021_01_08_425864 287 48 457 457 CD 10_1101-2021_01_08_425864 287 49 ( ( -LRB- 10_1101-2021_01_08_425864 287 50 Fig Fig NNP 10_1101-2021_01_08_425864 287 51 8D 8d NN 10_1101-2021_01_08_425864 287 52 ) ) -RRB- 10_1101-2021_01_08_425864 287 53 . . . 10_1101-2021_01_08_425864 288 1 To to TO 10_1101-2021_01_08_425864 288 2 reinforce reinforce VB 10_1101-2021_01_08_425864 288 3 the the DT 10_1101-2021_01_08_425864 288 4 validation validation NN 10_1101-2021_01_08_425864 288 5 of of IN 10_1101-2021_01_08_425864 288 6 ETO ETO NNP 10_1101-2021_01_08_425864 288 7 for for IN 10_1101-2021_01_08_425864 288 8 further further JJ 10_1101-2021_01_08_425864 288 9 experiments experiment NNS 10_1101-2021_01_08_425864 288 10 , , , 10_1101-2021_01_08_425864 288 11 a a DT 10_1101-2021_01_08_425864 288 12 set set NN 10_1101-2021_01_08_425864 288 13 of of IN 10_1101-2021_01_08_425864 288 14 controls control NNS 10_1101-2021_01_08_425864 288 15 are be VBP 10_1101-2021_01_08_425864 288 16 offered offer VBN 10_1101-2021_01_08_425864 288 17 in in IN 10_1101-2021_01_08_425864 288 18 458 458 CD 10_1101-2021_01_08_425864 288 19 S3 S3 NNP 10_1101-2021_01_08_425864 288 20 Fig Fig NNP 10_1101-2021_01_08_425864 288 21 . . . 10_1101-2021_01_08_425864 289 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 289 2 preliminary preliminary JJ 10_1101-2021_01_08_425864 289 3 conclusion conclusion NN 10_1101-2021_01_08_425864 289 4 is be VBZ 10_1101-2021_01_08_425864 289 5 that that IN 10_1101-2021_01_08_425864 289 6 ETO ETO NNP 10_1101-2021_01_08_425864 289 7 inhibited inhibit VBD 10_1101-2021_01_08_425864 289 8 beta beta NN 10_1101-2021_01_08_425864 289 9 - - HYPH 10_1101-2021_01_08_425864 289 10 oxidation oxidation NN 10_1101-2021_01_08_425864 289 11 by by IN 10_1101-2021_01_08_425864 289 12 inhibiting inhibit VBG 10_1101-2021_01_08_425864 289 13 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 289 14 , , , 10_1101-2021_01_08_425864 289 15 459 459 CD 10_1101-2021_01_08_425864 289 16 confirming confirm VBG 10_1101-2021_01_08_425864 289 17 that that IN 10_1101-2021_01_08_425864 289 18 the the DT 10_1101-2021_01_08_425864 289 19 breakdown breakdown NN 10_1101-2021_01_08_425864 289 20 of of IN 10_1101-2021_01_08_425864 289 21 fatty fatty NN 10_1101-2021_01_08_425864 289 22 acids acid NNS 10_1101-2021_01_08_425864 289 23 is be VBZ 10_1101-2021_01_08_425864 289 24 important important JJ 10_1101-2021_01_08_425864 289 25 to to IN 10_1101-2021_01_08_425864 289 26 proliferation proliferation NN 10_1101-2021_01_08_425864 289 27 progression progression NN 10_1101-2021_01_08_425864 289 28 in in IN 10_1101-2021_01_08_425864 289 29 the the DT 10_1101-2021_01_08_425864 289 30 absence absence NN 10_1101-2021_01_08_425864 289 31 460 460 CD 10_1101-2021_01_08_425864 289 32 of of IN 10_1101-2021_01_08_425864 289 33 glucose glucose NN 10_1101-2021_01_08_425864 289 34 . . . 10_1101-2021_01_08_425864 290 1 461 461 CD 10_1101-2021_01_08_425864 290 2 462 462 CD 10_1101-2021_01_08_425864 290 3 Figure figure NN 10_1101-2021_01_08_425864 290 4 8 8 CD 10_1101-2021_01_08_425864 290 5 . . . 10_1101-2021_01_08_425864 291 1 ETO ETO NNP 10_1101-2021_01_08_425864 291 2 inhibits inhibit VBZ 10_1101-2021_01_08_425864 291 3 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 291 4 and and CC 10_1101-2021_01_08_425864 291 5 interferes interfere VBZ 10_1101-2021_01_08_425864 291 6 with with IN 10_1101-2021_01_08_425864 291 7 cell cell NN 10_1101-2021_01_08_425864 291 8 proliferation proliferation NN 10_1101-2021_01_08_425864 291 9 in in IN 10_1101-2021_01_08_425864 291 10 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 291 11 forms form NNS 10_1101-2021_01_08_425864 291 12 . . . 10_1101-2021_01_08_425864 292 1 ( ( -LRB- 10_1101-2021_01_08_425864 292 2 A a NN 10_1101-2021_01_08_425864 292 3 ) ) -RRB- 10_1101-2021_01_08_425864 292 4 463 463 CD 10_1101-2021_01_08_425864 292 5 Proliferation proliferation NN 10_1101-2021_01_08_425864 292 6 of of IN 10_1101-2021_01_08_425864 292 7 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 292 8 forms form NNS 10_1101-2021_01_08_425864 292 9 in in IN 10_1101-2021_01_08_425864 292 10 the the DT 10_1101-2021_01_08_425864 292 11 presence presence NN 10_1101-2021_01_08_425864 292 12 of of IN 10_1101-2021_01_08_425864 292 13 0.1 0.1 CD 10_1101-2021_01_08_425864 292 14 to to TO 10_1101-2021_01_08_425864 292 15 500 500 CD 10_1101-2021_01_08_425864 292 16 µM µM NNP 10_1101-2021_01_08_425864 292 17 ETO ETO NNP 10_1101-2021_01_08_425864 292 18 . . . 10_1101-2021_01_08_425864 293 1 For for IN 10_1101-2021_01_08_425864 293 2 the the DT 10_1101-2021_01_08_425864 293 3 positive positive JJ 10_1101-2021_01_08_425864 293 4 control control NN 10_1101-2021_01_08_425864 293 5 464 464 CD 10_1101-2021_01_08_425864 293 6 of of IN 10_1101-2021_01_08_425864 293 7 dead dead JJ 10_1101-2021_01_08_425864 293 8 cells cell NNS 10_1101-2021_01_08_425864 293 9 , , , 10_1101-2021_01_08_425864 293 10 a a DT 10_1101-2021_01_08_425864 293 11 combination combination NN 10_1101-2021_01_08_425864 293 12 of of IN 10_1101-2021_01_08_425864 293 13 antimycin antimycin NNS 10_1101-2021_01_08_425864 293 14 ( ( -LRB- 10_1101-2021_01_08_425864 293 15 0.5 0.5 CD 10_1101-2021_01_08_425864 293 16 µM µM NNP 10_1101-2021_01_08_425864 293 17 ) ) -RRB- 10_1101-2021_01_08_425864 293 18 and and CC 10_1101-2021_01_08_425864 293 19 rotenone rotenone NN 10_1101-2021_01_08_425864 293 20 ( ( -LRB- 10_1101-2021_01_08_425864 293 21 60 60 CD 10_1101-2021_01_08_425864 293 22 µM µM NNP 10_1101-2021_01_08_425864 293 23 ) ) -RRB- 10_1101-2021_01_08_425864 293 24 was be VBD 10_1101-2021_01_08_425864 293 25 used use VBN 10_1101-2021_01_08_425864 293 26 . . . 10_1101-2021_01_08_425864 294 1 ( ( -LRB- 10_1101-2021_01_08_425864 294 2 B b NN 10_1101-2021_01_08_425864 294 3 ) ) -RRB- 10_1101-2021_01_08_425864 294 4 Inhibition inhibition NN 10_1101-2021_01_08_425864 294 5 of of IN 10_1101-2021_01_08_425864 294 6 465 465 CD 10_1101-2021_01_08_425864 294 7 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 294 8 activity activity NN 10_1101-2021_01_08_425864 294 9 in in IN 10_1101-2021_01_08_425864 294 10 crude crude JJ 10_1101-2021_01_08_425864 294 11 extracts extract NNS 10_1101-2021_01_08_425864 294 12 using use VBG 10_1101-2021_01_08_425864 294 13 250 250 CD 10_1101-2021_01_08_425864 294 14 and and CC 10_1101-2021_01_08_425864 294 15 500 500 CD 10_1101-2021_01_08_425864 294 16 µM µM NNP 10_1101-2021_01_08_425864 294 17 of of IN 10_1101-2021_01_08_425864 294 18 ETO ETO NNP 10_1101-2021_01_08_425864 294 19 . . . 10_1101-2021_01_08_425864 295 1 C C NNP 10_1101-2021_01_08_425864 295 2 ) ) -RRB- 10_1101-2021_01_08_425864 295 3 14CO2 14co2 CD 10_1101-2021_01_08_425864 295 4 capture capture NN 10_1101-2021_01_08_425864 295 5 from from IN 10_1101-2021_01_08_425864 295 6 14C 14c CD 10_1101-2021_01_08_425864 295 7 - - HYPH 10_1101-2021_01_08_425864 295 8 U- u- NN 10_1101-2021_01_08_425864 295 9 466 466 CD 10_1101-2021_01_08_425864 295 10 palmitate palmitate NN 10_1101-2021_01_08_425864 295 11 oxidation oxidation NN 10_1101-2021_01_08_425864 295 12 . . . 10_1101-2021_01_08_425864 296 1 D D NNP 10_1101-2021_01_08_425864 296 2 ) ) -RRB- 10_1101-2021_01_08_425864 296 3 Flow flow NN 10_1101-2021_01_08_425864 296 4 cytometry cytometry NN 10_1101-2021_01_08_425864 296 5 analysis analysis NN 10_1101-2021_01_08_425864 296 6 and and CC 10_1101-2021_01_08_425864 296 7 fluorescence fluorescence NN 10_1101-2021_01_08_425864 296 8 microscopy microscopy JJ 10_1101-2021_01_08_425864 296 9 of of IN 10_1101-2021_01_08_425864 296 10 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 296 11 forms form NNS 10_1101-2021_01_08_425864 296 12 467 467 CD 10_1101-2021_01_08_425864 296 13 treated treat VBN 10_1101-2021_01_08_425864 296 14 ( ( -LRB- 10_1101-2021_01_08_425864 296 15 or or CC 10_1101-2021_01_08_425864 296 16 not not RB 10_1101-2021_01_08_425864 296 17 ) ) -RRB- 10_1101-2021_01_08_425864 296 18 with with IN 10_1101-2021_01_08_425864 296 19 ETO ETO NNP 10_1101-2021_01_08_425864 296 20 . . . 10_1101-2021_01_08_425864 297 1 In in IN 10_1101-2021_01_08_425864 297 2 the the DT 10_1101-2021_01_08_425864 297 3 histograms histogram NNS 10_1101-2021_01_08_425864 297 4 , , , 10_1101-2021_01_08_425864 297 5 dashed dash VBD 10_1101-2021_01_08_425864 297 6 peaks peak NNS 10_1101-2021_01_08_425864 297 7 represent represent VBP 10_1101-2021_01_08_425864 297 8 unstained unstained JJ 10_1101-2021_01_08_425864 297 9 parasites parasite NNS 10_1101-2021_01_08_425864 297 10 and and CC 10_1101-2021_01_08_425864 297 11 green- green- XX 10_1101-2021_01_08_425864 297 12 468 468 CD 10_1101-2021_01_08_425864 297 13 filled fill VBD 10_1101-2021_01_08_425864 297 14 peaks peak NNS 10_1101-2021_01_08_425864 297 15 represent represent VBP 10_1101-2021_01_08_425864 297 16 parasites parasite NNS 10_1101-2021_01_08_425864 297 17 stained stain VBN 10_1101-2021_01_08_425864 297 18 with with IN 10_1101-2021_01_08_425864 297 19 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 297 20 C1-C12 C1-C12 NNP 10_1101-2021_01_08_425864 297 21 . . . 10_1101-2021_01_08_425864 298 1 All all PDT 10_1101-2021_01_08_425864 298 2 the the DT 10_1101-2021_01_08_425864 298 3 experiments experiment NNS 10_1101-2021_01_08_425864 298 4 were be VBD 10_1101-2021_01_08_425864 298 5 performed perform VBN 10_1101-2021_01_08_425864 298 6 in in IN 10_1101-2021_01_08_425864 298 7 .CC .CC NFP 10_1101-2021_01_08_425864 298 8 - - : 10_1101-2021_01_08_425864 298 9 BY by IN 10_1101-2021_01_08_425864 298 10 4.0 4.0 CD 10_1101-2021_01_08_425864 298 11 International international JJ 10_1101-2021_01_08_425864 298 12 licensemade licensemade NN 10_1101-2021_01_08_425864 298 13 available available JJ 10_1101-2021_01_08_425864 298 14 under under IN 10_1101-2021_01_08_425864 298 15 a a DT 10_1101-2021_01_08_425864 298 16 ( ( -LRB- 10_1101-2021_01_08_425864 298 17 which which WDT 10_1101-2021_01_08_425864 298 18 was be VBD 10_1101-2021_01_08_425864 298 19 not not RB 10_1101-2021_01_08_425864 298 20 certified certify VBN 10_1101-2021_01_08_425864 298 21 by by IN 10_1101-2021_01_08_425864 298 22 peer peer NN 10_1101-2021_01_08_425864 298 23 review review NN 10_1101-2021_01_08_425864 298 24 ) ) -RRB- 10_1101-2021_01_08_425864 298 25 is be VBZ 10_1101-2021_01_08_425864 298 26 the the DT 10_1101-2021_01_08_425864 298 27 author author NN 10_1101-2021_01_08_425864 298 28 / / SYM 10_1101-2021_01_08_425864 298 29 funder funder NN 10_1101-2021_01_08_425864 298 30 , , , 10_1101-2021_01_08_425864 298 31 who who WP 10_1101-2021_01_08_425864 298 32 has have VBZ 10_1101-2021_01_08_425864 298 33 granted grant VBN 10_1101-2021_01_08_425864 298 34 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 298 35 a a DT 10_1101-2021_01_08_425864 298 36 license license NN 10_1101-2021_01_08_425864 298 37 to to TO 10_1101-2021_01_08_425864 298 38 display display VB 10_1101-2021_01_08_425864 298 39 the the DT 10_1101-2021_01_08_425864 298 40 preprint preprint NN 10_1101-2021_01_08_425864 298 41 in in IN 10_1101-2021_01_08_425864 298 42 perpetuity perpetuity NN 10_1101-2021_01_08_425864 298 43 . . . 10_1101-2021_01_08_425864 299 1 It -PRON- PRP 10_1101-2021_01_08_425864 299 2 is be VBZ 10_1101-2021_01_08_425864 299 3 The the DT 10_1101-2021_01_08_425864 299 4 copyright copyright NN 10_1101-2021_01_08_425864 299 5 holder holder NN 10_1101-2021_01_08_425864 299 6 for for IN 10_1101-2021_01_08_425864 299 7 this this DT 10_1101-2021_01_08_425864 299 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 299 9 version version NN 10_1101-2021_01_08_425864 299 10 posted post VBD 10_1101-2021_01_08_425864 299 11 January January NNP 10_1101-2021_01_08_425864 299 12 8 8 CD 10_1101-2021_01_08_425864 299 13 , , , 10_1101-2021_01_08_425864 299 14 2021 2021 CD 10_1101-2021_01_08_425864 299 15 . . . 10_1101-2021_01_08_425864 299 16 ; ; : 10_1101-2021_01_08_425864 299 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 299 18 : : : 10_1101-2021_01_08_425864 299 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 299 20 preprint preprint NN 10_1101-2021_01_08_425864 299 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 299 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 299 23 469 469 CD 10_1101-2021_01_08_425864 299 24 triplicates triplicate NNS 10_1101-2021_01_08_425864 299 25 . . . 10_1101-2021_01_08_425864 300 1 Statistical statistical JJ 10_1101-2021_01_08_425864 300 2 analysis analysis NN 10_1101-2021_01_08_425864 300 3 was be VBD 10_1101-2021_01_08_425864 300 4 performed perform VBN 10_1101-2021_01_08_425864 300 5 with with IN 10_1101-2021_01_08_425864 300 6 one one CD 10_1101-2021_01_08_425864 300 7 - - HYPH 10_1101-2021_01_08_425864 300 8 way way NN 10_1101-2021_01_08_425864 300 9 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 300 10 followed follow VBN 10_1101-2021_01_08_425864 300 11 by by IN 10_1101-2021_01_08_425864 300 12 Tukey Tukey NNP 10_1101-2021_01_08_425864 300 13 's 's POS 10_1101-2021_01_08_425864 300 14 post post JJ 10_1101-2021_01_08_425864 300 15 - - JJ 10_1101-2021_01_08_425864 300 16 test test NN 10_1101-2021_01_08_425864 300 17 470 470 CD 10_1101-2021_01_08_425864 300 18 at at IN 10_1101-2021_01_08_425864 300 19 p p NN 10_1101-2021_01_08_425864 300 20 < < XX 10_1101-2021_01_08_425864 300 21 0.05 0.05 XX 10_1101-2021_01_08_425864 300 22 using use VBG 10_1101-2021_01_08_425864 300 23 the the DT 10_1101-2021_01_08_425864 300 24 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 300 25 Prism Prism NNP 10_1101-2021_01_08_425864 300 26 8.0.2 8.0.2 NNP 10_1101-2021_01_08_425864 300 27 software software NN 10_1101-2021_01_08_425864 300 28 program program NN 10_1101-2021_01_08_425864 300 29 . . . 10_1101-2021_01_08_425864 301 1 We -PRON- PRP 10_1101-2021_01_08_425864 301 2 represent represent VBP 10_1101-2021_01_08_425864 301 3 the the DT 10_1101-2021_01_08_425864 301 4 level level NN 10_1101-2021_01_08_425864 301 5 of of IN 10_1101-2021_01_08_425864 301 6 statistical statistical JJ 10_1101-2021_01_08_425864 301 7 471 471 CD 10_1101-2021_01_08_425864 301 8 significance significance NN 10_1101-2021_01_08_425864 301 9 in in IN 10_1101-2021_01_08_425864 301 10 this this DT 10_1101-2021_01_08_425864 301 11 figure figure NN 10_1101-2021_01_08_425864 301 12 as as IN 10_1101-2021_01_08_425864 301 13 follows follow VBZ 10_1101-2021_01_08_425864 301 14 : : : 10_1101-2021_01_08_425864 301 15 * * NFP 10_1101-2021_01_08_425864 301 16 * * NFP 10_1101-2021_01_08_425864 301 17 * * NFP 10_1101-2021_01_08_425864 301 18 p p NN 10_1101-2021_01_08_425864 301 19 value value NN 10_1101-2021_01_08_425864 301 20 < < XX 10_1101-2021_01_08_425864 301 21 0.001 0.001 CD 10_1101-2021_01_08_425864 301 22 ; ; : 10_1101-2021_01_08_425864 301 23 * * NFP 10_1101-2021_01_08_425864 301 24 * * NFP 10_1101-2021_01_08_425864 301 25 p p NN 10_1101-2021_01_08_425864 301 26 value value NN 10_1101-2021_01_08_425864 301 27 < < XX 10_1101-2021_01_08_425864 301 28 0.01 0.01 XX 10_1101-2021_01_08_425864 301 29 ; ; : 10_1101-2021_01_08_425864 301 30 and and CC 10_1101-2021_01_08_425864 301 31 * * NFP 10_1101-2021_01_08_425864 301 32 p p NN 10_1101-2021_01_08_425864 301 33 value value NN 10_1101-2021_01_08_425864 301 34 < < XX 10_1101-2021_01_08_425864 301 35 0.05 0.05 CD 10_1101-2021_01_08_425864 301 36 . . . 10_1101-2021_01_08_425864 302 1 472 472 CD 10_1101-2021_01_08_425864 302 2 For for IN 10_1101-2021_01_08_425864 302 3 p p NN 10_1101-2021_01_08_425864 302 4 values value NNS 10_1101-2021_01_08_425864 302 5 > > XX 10_1101-2021_01_08_425864 302 6 0.05 0.05 CD 10_1101-2021_01_08_425864 302 7 , , , 10_1101-2021_01_08_425864 302 8 we -PRON- PRP 10_1101-2021_01_08_425864 302 9 consider consider VBP 10_1101-2021_01_08_425864 302 10 the the DT 10_1101-2021_01_08_425864 302 11 differences difference NNS 10_1101-2021_01_08_425864 302 12 not not RB 10_1101-2021_01_08_425864 302 13 significant significant JJ 10_1101-2021_01_08_425864 302 14 ( ( -LRB- 10_1101-2021_01_08_425864 302 15 ns ns NNP 10_1101-2021_01_08_425864 302 16 ) ) -RRB- 10_1101-2021_01_08_425864 302 17 . . . 10_1101-2021_01_08_425864 303 1 473 473 CD 10_1101-2021_01_08_425864 303 2 474 474 CD 10_1101-2021_01_08_425864 303 3 Etomoxir Etomoxir NNP 10_1101-2021_01_08_425864 303 4 treatment treatment NN 10_1101-2021_01_08_425864 303 5 affects affect VBZ 10_1101-2021_01_08_425864 303 6 cell cell NN 10_1101-2021_01_08_425864 303 7 cycle cycle NN 10_1101-2021_01_08_425864 303 8 progression progression NN 10_1101-2021_01_08_425864 303 9 475 475 CD 10_1101-2021_01_08_425864 303 10 The the DT 10_1101-2021_01_08_425864 303 11 metabolic metabolic JJ 10_1101-2021_01_08_425864 303 12 interference interference NN 10_1101-2021_01_08_425864 303 13 of of IN 10_1101-2021_01_08_425864 303 14 ETO ETO NNP 10_1101-2021_01_08_425864 303 15 diminished diminish VBD 10_1101-2021_01_08_425864 303 16 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 303 17 proliferation proliferation NN 10_1101-2021_01_08_425864 303 18 ; ; : 10_1101-2021_01_08_425864 303 19 however however RB 10_1101-2021_01_08_425864 303 20 , , , 10_1101-2021_01_08_425864 303 21 this this DT 10_1101-2021_01_08_425864 303 22 476 476 CD 10_1101-2021_01_08_425864 303 23 finding finding NN 10_1101-2021_01_08_425864 303 24 could could MD 10_1101-2021_01_08_425864 303 25 be be VB 10_1101-2021_01_08_425864 303 26 due due IN 10_1101-2021_01_08_425864 303 27 to to IN 10_1101-2021_01_08_425864 303 28 a a DT 10_1101-2021_01_08_425864 303 29 decrease decrease NN 10_1101-2021_01_08_425864 303 30 in in IN 10_1101-2021_01_08_425864 303 31 the the DT 10_1101-2021_01_08_425864 303 32 parasite parasite NN 10_1101-2021_01_08_425864 303 33 proliferation proliferation NN 10_1101-2021_01_08_425864 303 34 rate rate NN 10_1101-2021_01_08_425864 303 35 or or CC 10_1101-2021_01_08_425864 303 36 an an DT 10_1101-2021_01_08_425864 303 37 increase increase NN 10_1101-2021_01_08_425864 303 38 in in IN 10_1101-2021_01_08_425864 303 39 the the DT 10_1101-2021_01_08_425864 303 40 death death NN 10_1101-2021_01_08_425864 303 41 rate rate NN 10_1101-2021_01_08_425864 303 42 . . . 10_1101-2021_01_08_425864 304 1 477 477 CD 10_1101-2021_01_08_425864 304 2 Therefore therefore RB 10_1101-2021_01_08_425864 304 3 , , , 10_1101-2021_01_08_425864 304 4 we -PRON- PRP 10_1101-2021_01_08_425864 304 5 checked check VBD 10_1101-2021_01_08_425864 304 6 if if IN 10_1101-2021_01_08_425864 304 7 this this DT 10_1101-2021_01_08_425864 304 8 compound compound NN 10_1101-2021_01_08_425864 304 9 could could MD 10_1101-2021_01_08_425864 304 10 induce induce VB 10_1101-2021_01_08_425864 304 11 cell cell NN 10_1101-2021_01_08_425864 304 12 death death NN 10_1101-2021_01_08_425864 304 13 through through IN 10_1101-2021_01_08_425864 304 14 programmed program VBN 10_1101-2021_01_08_425864 304 15 cell cell NN 10_1101-2021_01_08_425864 304 16 death death NN 10_1101-2021_01_08_425864 304 17 478 478 CD 10_1101-2021_01_08_425864 304 18 ( ( -LRB- 10_1101-2021_01_08_425864 304 19 PCD PCD NNP 10_1101-2021_01_08_425864 304 20 ) ) -RRB- 10_1101-2021_01_08_425864 304 21 or or CC 10_1101-2021_01_08_425864 304 22 necrosis necrosis NN 10_1101-2021_01_08_425864 304 23 . . . 10_1101-2021_01_08_425864 305 1 PCD PCD NNP 10_1101-2021_01_08_425864 305 2 is be VBZ 10_1101-2021_01_08_425864 305 3 characterized characterize VBN 10_1101-2021_01_08_425864 305 4 by by IN 10_1101-2021_01_08_425864 305 5 biochemical biochemical JJ 10_1101-2021_01_08_425864 305 6 and and CC 10_1101-2021_01_08_425864 305 7 morphological morphological JJ 10_1101-2021_01_08_425864 305 8 events event NNS 10_1101-2021_01_08_425864 305 9 such such JJ 10_1101-2021_01_08_425864 305 10 as as IN 10_1101-2021_01_08_425864 305 11 exposure exposure NN 10_1101-2021_01_08_425864 305 12 479 479 CD 10_1101-2021_01_08_425864 305 13 to to TO 10_1101-2021_01_08_425864 305 14 phosphatidylserine phosphatidylserine VB 10_1101-2021_01_08_425864 305 15 , , , 10_1101-2021_01_08_425864 305 16 DNA dna NN 10_1101-2021_01_08_425864 305 17 fragmentation fragmentation NN 10_1101-2021_01_08_425864 305 18 , , , 10_1101-2021_01_08_425864 305 19 decreases decrease VBZ 10_1101-2021_01_08_425864 305 20 ( ( -LRB- 10_1101-2021_01_08_425864 305 21 or or CC 10_1101-2021_01_08_425864 305 22 increases increase NNS 10_1101-2021_01_08_425864 305 23 ) ) -RRB- 10_1101-2021_01_08_425864 305 24 in in IN 10_1101-2021_01_08_425864 305 25 the the DT 10_1101-2021_01_08_425864 305 26 ATP ATP NNP 10_1101-2021_01_08_425864 305 27 levels level NNS 10_1101-2021_01_08_425864 305 28 , , , 10_1101-2021_01_08_425864 305 29 and and CC 10_1101-2021_01_08_425864 305 30 increases increase VBZ 10_1101-2021_01_08_425864 305 31 480 480 CD 10_1101-2021_01_08_425864 305 32 in in IN 10_1101-2021_01_08_425864 305 33 reactive reactive JJ 10_1101-2021_01_08_425864 305 34 oxygen oxygen NN 10_1101-2021_01_08_425864 305 35 species specie NNS 10_1101-2021_01_08_425864 305 36 ( ( -LRB- 10_1101-2021_01_08_425864 305 37 ROS ROS NNP 10_1101-2021_01_08_425864 305 38 ) ) -RRB- 10_1101-2021_01_08_425864 305 39 , , , 10_1101-2021_01_08_425864 305 40 among among IN 10_1101-2021_01_08_425864 305 41 others other NNS 10_1101-2021_01_08_425864 305 42 [ [ -LRB- 10_1101-2021_01_08_425864 305 43 33 33 CD 10_1101-2021_01_08_425864 305 44 ] ] -RRB- 10_1101-2021_01_08_425864 305 45 . . . 10_1101-2021_01_08_425864 306 1 The the DT 10_1101-2021_01_08_425864 306 2 parasites parasite NNS 10_1101-2021_01_08_425864 306 3 were be VBD 10_1101-2021_01_08_425864 306 4 treated treat VBN 10_1101-2021_01_08_425864 306 5 with with IN 10_1101-2021_01_08_425864 306 6 500 500 CD 10_1101-2021_01_08_425864 306 7 µM µM NNP 10_1101-2021_01_08_425864 306 8 of of IN 10_1101-2021_01_08_425864 306 9 481 481 CD 10_1101-2021_01_08_425864 306 10 ETO ETO NNS 10_1101-2021_01_08_425864 306 11 for for IN 10_1101-2021_01_08_425864 306 12 5 5 CD 10_1101-2021_01_08_425864 306 13 days day NNS 10_1101-2021_01_08_425864 306 14 , , , 10_1101-2021_01_08_425864 306 15 followed follow VBN 10_1101-2021_01_08_425864 306 16 by by IN 10_1101-2021_01_08_425864 306 17 incubation incubation NN 10_1101-2021_01_08_425864 306 18 with with IN 10_1101-2021_01_08_425864 306 19 propidium propidium NN 10_1101-2021_01_08_425864 306 20 iodide iodide NN 10_1101-2021_01_08_425864 306 21 ( ( -LRB- 10_1101-2021_01_08_425864 306 22 PI PI NNP 10_1101-2021_01_08_425864 306 23 ) ) -RRB- 10_1101-2021_01_08_425864 306 24 for for IN 10_1101-2021_01_08_425864 306 25 cell cell NN 10_1101-2021_01_08_425864 306 26 membrane membrane NN 10_1101-2021_01_08_425864 306 27 integrity integrity NN 10_1101-2021_01_08_425864 306 28 482 482 CD 10_1101-2021_01_08_425864 306 29 analysis analysis NN 10_1101-2021_01_08_425864 306 30 and and CC 10_1101-2021_01_08_425864 306 31 annexin annexin NN 10_1101-2021_01_08_425864 306 32 - - HYPH 10_1101-2021_01_08_425864 306 33 V v NN 10_1101-2021_01_08_425864 306 34 FITC FITC NNP 10_1101-2021_01_08_425864 306 35 to to TO 10_1101-2021_01_08_425864 306 36 evaluate evaluate VB 10_1101-2021_01_08_425864 306 37 the the DT 10_1101-2021_01_08_425864 306 38 phosphatidylserine phosphatidylserine NN 10_1101-2021_01_08_425864 306 39 exposure exposure NN 10_1101-2021_01_08_425864 306 40 . . . 10_1101-2021_01_08_425864 307 1 Parasites parasite NNS 10_1101-2021_01_08_425864 307 2 treated treat VBD 10_1101-2021_01_08_425864 307 3 with with IN 10_1101-2021_01_08_425864 307 4 ETO ETO NNP 10_1101-2021_01_08_425864 307 5 483 483 CD 10_1101-2021_01_08_425864 307 6 showed show VBD 10_1101-2021_01_08_425864 307 7 negative negative JJ 10_1101-2021_01_08_425864 307 8 results result NNS 10_1101-2021_01_08_425864 307 9 for for IN 10_1101-2021_01_08_425864 307 10 necrosis necrosis NN 10_1101-2021_01_08_425864 307 11 or or CC 10_1101-2021_01_08_425864 307 12 programmed program VBN 10_1101-2021_01_08_425864 307 13 cell cell NN 10_1101-2021_01_08_425864 307 14 death death NN 10_1101-2021_01_08_425864 307 15 markers marker NNS 10_1101-2021_01_08_425864 307 16 ( ( -LRB- 10_1101-2021_01_08_425864 307 17 Fig fig NN 10_1101-2021_01_08_425864 307 18 9A 9a NN 10_1101-2021_01_08_425864 307 19 ) ) -RRB- 10_1101-2021_01_08_425864 307 20 , , , 10_1101-2021_01_08_425864 307 21 indicating indicate VBG 10_1101-2021_01_08_425864 307 22 that that IN 10_1101-2021_01_08_425864 307 23 the the DT 10_1101-2021_01_08_425864 307 24 484 484 CD 10_1101-2021_01_08_425864 307 25 cell cell NN 10_1101-2021_01_08_425864 307 26 proliferation proliferation NN 10_1101-2021_01_08_425864 307 27 was be VBD 10_1101-2021_01_08_425864 307 28 arrested arrest VBN 10_1101-2021_01_08_425864 307 29 but but CC 10_1101-2021_01_08_425864 307 30 cell cell NN 10_1101-2021_01_08_425864 307 31 viability viability NN 10_1101-2021_01_08_425864 307 32 was be VBD 10_1101-2021_01_08_425864 307 33 maintained maintain VBN 10_1101-2021_01_08_425864 307 34 . . . 10_1101-2021_01_08_425864 308 1 Because because IN 10_1101-2021_01_08_425864 308 2 the the DT 10_1101-2021_01_08_425864 308 3 multiplication multiplication NN 10_1101-2021_01_08_425864 308 4 rates rate NNS 10_1101-2021_01_08_425864 308 5 485 485 CD 10_1101-2021_01_08_425864 308 6 seemed seem VBD 10_1101-2021_01_08_425864 308 7 to to TO 10_1101-2021_01_08_425864 308 8 be be VB 10_1101-2021_01_08_425864 308 9 diminished diminish VBN 10_1101-2021_01_08_425864 308 10 , , , 10_1101-2021_01_08_425864 308 11 we -PRON- PRP 10_1101-2021_01_08_425864 308 12 performed perform VBD 10_1101-2021_01_08_425864 308 13 a a DT 10_1101-2021_01_08_425864 308 14 cell cell NN 10_1101-2021_01_08_425864 308 15 cycle cycle NN 10_1101-2021_01_08_425864 308 16 analysis analysis NN 10_1101-2021_01_08_425864 308 17 . . . 10_1101-2021_01_08_425864 309 1 Noticeably noticeably RB 10_1101-2021_01_08_425864 309 2 , , , 10_1101-2021_01_08_425864 309 3 the the DT 10_1101-2021_01_08_425864 309 4 treated treat VBN 10_1101-2021_01_08_425864 309 5 parasites parasite NNS 10_1101-2021_01_08_425864 309 6 were be VBD 10_1101-2021_01_08_425864 309 7 486 486 CD 10_1101-2021_01_08_425864 309 8 enriched enrich VBN 10_1101-2021_01_08_425864 309 9 in in IN 10_1101-2021_01_08_425864 309 10 G1 G1 NNP 10_1101-2021_01_08_425864 309 11 ( ( -LRB- 10_1101-2021_01_08_425864 309 12 85.9 85.9 CD 10_1101-2021_01_08_425864 309 13 % % NN 10_1101-2021_01_08_425864 309 14 ) ) -RRB- 10_1101-2021_01_08_425864 309 15 with with IN 10_1101-2021_01_08_425864 309 16 respect respect NN 10_1101-2021_01_08_425864 309 17 to to IN 10_1101-2021_01_08_425864 309 18 non non JJ 10_1101-2021_01_08_425864 309 19 - - JJ 10_1101-2021_01_08_425864 309 20 treated treated JJ 10_1101-2021_01_08_425864 309 21 cells cell NNS 10_1101-2021_01_08_425864 309 22 ( ( -LRB- 10_1101-2021_01_08_425864 309 23 43.6 43.6 CD 10_1101-2021_01_08_425864 309 24 % % NN 10_1101-2021_01_08_425864 309 25 ) ) -RRB- 10_1101-2021_01_08_425864 309 26 , , , 10_1101-2021_01_08_425864 309 27 suggesting suggest VBG 10_1101-2021_01_08_425864 309 28 that that IN 10_1101-2021_01_08_425864 309 29 ETO ETO NNP 10_1101-2021_01_08_425864 309 30 prevented prevent VBD 10_1101-2021_01_08_425864 309 31 the the DT 10_1101-2021_01_08_425864 309 32 487 487 CD 10_1101-2021_01_08_425864 309 33 entry entry NN 10_1101-2021_01_08_425864 309 34 of of IN 10_1101-2021_01_08_425864 309 35 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 309 36 into into IN 10_1101-2021_01_08_425864 309 37 the the DT 10_1101-2021_01_08_425864 309 38 S S NNP 10_1101-2021_01_08_425864 309 39 phase phase NN 10_1101-2021_01_08_425864 309 40 of of IN 10_1101-2021_01_08_425864 309 41 the the DT 10_1101-2021_01_08_425864 309 42 cell cell NN 10_1101-2021_01_08_425864 309 43 cycle cycle NN 10_1101-2021_01_08_425864 309 44 ( ( -LRB- 10_1101-2021_01_08_425864 309 45 Fig Fig NNP 10_1101-2021_01_08_425864 309 46 9B 9b NN 10_1101-2021_01_08_425864 309 47 ) ) -RRB- 10_1101-2021_01_08_425864 309 48 . . . 10_1101-2021_01_08_425864 310 1 Last last JJ 10_1101-2021_01_08_425864 310 2 , , , 10_1101-2021_01_08_425864 310 3 we -PRON- PRP 10_1101-2021_01_08_425864 310 4 noticed notice VBD 10_1101-2021_01_08_425864 310 5 that that IN 10_1101-2021_01_08_425864 310 6 after after IN 10_1101-2021_01_08_425864 310 7 washing wash VBG 10_1101-2021_01_08_425864 310 8 488 488 CD 10_1101-2021_01_08_425864 310 9 out out IN 10_1101-2021_01_08_425864 310 10 the the DT 10_1101-2021_01_08_425864 310 11 ETO ETO NNP 10_1101-2021_01_08_425864 310 12 , , , 10_1101-2021_01_08_425864 310 13 the the DT 10_1101-2021_01_08_425864 310 14 parasites parasite NNS 10_1101-2021_01_08_425864 310 15 recovered recover VBD 10_1101-2021_01_08_425864 310 16 their -PRON- PRP$ 10_1101-2021_01_08_425864 310 17 proliferation proliferation NN 10_1101-2021_01_08_425864 310 18 at at IN 10_1101-2021_01_08_425864 310 19 rates rate NNS 10_1101-2021_01_08_425864 310 20 comparable comparable JJ 10_1101-2021_01_08_425864 310 21 to to IN 10_1101-2021_01_08_425864 310 22 our -PRON- PRP$ 10_1101-2021_01_08_425864 310 23 untreated untreated JJ 10_1101-2021_01_08_425864 310 24 controls control NNS 10_1101-2021_01_08_425864 310 25 489 489 CD 10_1101-2021_01_08_425864 310 26 ( ( -LRB- 10_1101-2021_01_08_425864 310 27 Figs fig NNS 10_1101-2021_01_08_425864 310 28 9C 9c NN 10_1101-2021_01_08_425864 310 29 ) ) -RRB- 10_1101-2021_01_08_425864 310 30 . . . 10_1101-2021_01_08_425864 311 1 490 490 CD 10_1101-2021_01_08_425864 311 2 491 491 CD 10_1101-2021_01_08_425864 311 3 Figure figure NN 10_1101-2021_01_08_425864 311 4 9 9 CD 10_1101-2021_01_08_425864 311 5 . . . 10_1101-2021_01_08_425864 312 1 Analysis Analysis NNP 10_1101-2021_01_08_425864 312 2 of of IN 10_1101-2021_01_08_425864 312 3 extracellular extracellular NNP 10_1101-2021_01_08_425864 312 4 phosphatidylserine phosphatidylserine NN 10_1101-2021_01_08_425864 312 5 exposure exposure NN 10_1101-2021_01_08_425864 312 6 , , , 10_1101-2021_01_08_425864 312 7 membrane membrane NN 10_1101-2021_01_08_425864 312 8 integrity integrity NN 10_1101-2021_01_08_425864 312 9 and and CC 10_1101-2021_01_08_425864 312 10 cell cell NN 10_1101-2021_01_08_425864 312 11 492 492 CD 10_1101-2021_01_08_425864 312 12 cycle cycle NN 10_1101-2021_01_08_425864 312 13 after after IN 10_1101-2021_01_08_425864 312 14 ETO ETO NNP 10_1101-2021_01_08_425864 312 15 treatment treatment NN 10_1101-2021_01_08_425864 312 16 . . . 10_1101-2021_01_08_425864 313 1 Parasites parasite NNS 10_1101-2021_01_08_425864 313 2 in in IN 10_1101-2021_01_08_425864 313 3 the the DT 10_1101-2021_01_08_425864 313 4 exponential exponential JJ 10_1101-2021_01_08_425864 313 5 growth growth NN 10_1101-2021_01_08_425864 313 6 phase phase NN 10_1101-2021_01_08_425864 313 7 were be VBD 10_1101-2021_01_08_425864 313 8 treated treat VBN 10_1101-2021_01_08_425864 313 9 with with IN 10_1101-2021_01_08_425864 313 10 500 500 CD 10_1101-2021_01_08_425864 313 11 µM µM NNP 10_1101-2021_01_08_425864 313 12 of of IN 10_1101-2021_01_08_425864 313 13 493 493 CD 10_1101-2021_01_08_425864 313 14 ETO eto NN 10_1101-2021_01_08_425864 313 15 for for IN 10_1101-2021_01_08_425864 313 16 5 5 CD 10_1101-2021_01_08_425864 313 17 days day NNS 10_1101-2021_01_08_425864 313 18 . . . 10_1101-2021_01_08_425864 314 1 ( ( -LRB- 10_1101-2021_01_08_425864 314 2 A a NN 10_1101-2021_01_08_425864 314 3 ) ) -RRB- 10_1101-2021_01_08_425864 314 4 Following follow VBG 10_1101-2021_01_08_425864 314 5 the the DT 10_1101-2021_01_08_425864 314 6 incubation incubation NN 10_1101-2021_01_08_425864 314 7 period period NN 10_1101-2021_01_08_425864 314 8 , , , 10_1101-2021_01_08_425864 314 9 the the DT 10_1101-2021_01_08_425864 314 10 parasites parasite NNS 10_1101-2021_01_08_425864 314 11 were be VBD 10_1101-2021_01_08_425864 314 12 labelled label VBN 10_1101-2021_01_08_425864 314 13 with with IN 10_1101-2021_01_08_425864 314 14 propidium propidium NN 10_1101-2021_01_08_425864 314 15 494 494 CD 10_1101-2021_01_08_425864 314 16 iodide iodide NN 10_1101-2021_01_08_425864 314 17 ( ( -LRB- 10_1101-2021_01_08_425864 314 18 PI PI NNP 10_1101-2021_01_08_425864 314 19 ) ) -RRB- 10_1101-2021_01_08_425864 314 20 and and CC 10_1101-2021_01_08_425864 314 21 annexin annexin VB 10_1101-2021_01_08_425864 314 22 V V NNP 10_1101-2021_01_08_425864 314 23 - - HYPH 10_1101-2021_01_08_425864 314 24 FITC FITC NNP 10_1101-2021_01_08_425864 314 25 ( ( -LRB- 10_1101-2021_01_08_425864 314 26 ANX ANX NNP 10_1101-2021_01_08_425864 314 27 ) ) -RRB- 10_1101-2021_01_08_425864 314 28 and and CC 10_1101-2021_01_08_425864 314 29 analysed analyse VBN 10_1101-2021_01_08_425864 314 30 by by IN 10_1101-2021_01_08_425864 314 31 flow flow NN 10_1101-2021_01_08_425864 314 32 cytometry cytometry NN 10_1101-2021_01_08_425864 314 33 . . . 10_1101-2021_01_08_425864 315 1 ( ( -LRB- 10_1101-2021_01_08_425864 315 2 B b NN 10_1101-2021_01_08_425864 315 3 ) ) -RRB- 10_1101-2021_01_08_425864 315 4 The the DT 10_1101-2021_01_08_425864 315 5 cell cell NN 10_1101-2021_01_08_425864 315 6 cycle cycle NN 10_1101-2021_01_08_425864 315 7 was be VBD 10_1101-2021_01_08_425864 315 8 495 495 CD 10_1101-2021_01_08_425864 315 9 assessed assess VBD 10_1101-2021_01_08_425864 315 10 using use VBG 10_1101-2021_01_08_425864 315 11 PI PI NNP 10_1101-2021_01_08_425864 315 12 staining staining NN 10_1101-2021_01_08_425864 315 13 . . . 10_1101-2021_01_08_425864 316 1 ( ( -LRB- 10_1101-2021_01_08_425864 316 2 C c NN 10_1101-2021_01_08_425864 316 3 ) ) -RRB- 10_1101-2021_01_08_425864 316 4 Growth growth NN 10_1101-2021_01_08_425864 316 5 curves curve NNS 10_1101-2021_01_08_425864 316 6 of of IN 10_1101-2021_01_08_425864 316 7 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 316 8 forms form NNS 10_1101-2021_01_08_425864 316 9 before before RB 10_1101-2021_01_08_425864 316 10 and and CC 10_1101-2021_01_08_425864 316 11 after after IN 10_1101-2021_01_08_425864 316 12 removing remove VBG 10_1101-2021_01_08_425864 316 13 the the DT 10_1101-2021_01_08_425864 316 14 496 496 CD 10_1101-2021_01_08_425864 316 15 treatment treatment NN 10_1101-2021_01_08_425864 316 16 . . . 10_1101-2021_01_08_425864 317 1 All all PDT 10_1101-2021_01_08_425864 317 2 the the DT 10_1101-2021_01_08_425864 317 3 experiments experiment NNS 10_1101-2021_01_08_425864 317 4 were be VBD 10_1101-2021_01_08_425864 317 5 performed perform VBN 10_1101-2021_01_08_425864 317 6 in in IN 10_1101-2021_01_08_425864 317 7 triplicates triplicate NNS 10_1101-2021_01_08_425864 317 8 . . . 10_1101-2021_01_08_425864 318 1 Statistical statistical JJ 10_1101-2021_01_08_425864 318 2 analysis analysis NN 10_1101-2021_01_08_425864 318 3 was be VBD 10_1101-2021_01_08_425864 318 4 performed perform VBN 10_1101-2021_01_08_425864 318 5 with with IN 10_1101-2021_01_08_425864 318 6 497 497 CD 10_1101-2021_01_08_425864 318 7 one one CD 10_1101-2021_01_08_425864 318 8 - - HYPH 10_1101-2021_01_08_425864 318 9 way way NN 10_1101-2021_01_08_425864 318 10 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 318 11 followed follow VBN 10_1101-2021_01_08_425864 318 12 by by IN 10_1101-2021_01_08_425864 318 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 318 14 's 's POS 10_1101-2021_01_08_425864 318 15 post post JJ 10_1101-2021_01_08_425864 318 16 - - JJ 10_1101-2021_01_08_425864 318 17 test test JJ 10_1101-2021_01_08_425864 318 18 p p NN 10_1101-2021_01_08_425864 318 19 < < XX 10_1101-2021_01_08_425864 318 20 0.05 0.05 CD 10_1101-2021_01_08_425864 318 21 , , , 10_1101-2021_01_08_425864 318 22 using use VBG 10_1101-2021_01_08_425864 318 23 the the DT 10_1101-2021_01_08_425864 318 24 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 318 25 Prism Prism NNP 10_1101-2021_01_08_425864 318 26 8.0.2 8.0.2 CD 10_1101-2021_01_08_425864 318 27 software software NN 10_1101-2021_01_08_425864 318 28 498 498 CD 10_1101-2021_01_08_425864 318 29 program program NN 10_1101-2021_01_08_425864 318 30 . . . 10_1101-2021_01_08_425864 319 1 We -PRON- PRP 10_1101-2021_01_08_425864 319 2 represent represent VBP 10_1101-2021_01_08_425864 319 3 the the DT 10_1101-2021_01_08_425864 319 4 level level NN 10_1101-2021_01_08_425864 319 5 of of IN 10_1101-2021_01_08_425864 319 6 statistical statistical JJ 10_1101-2021_01_08_425864 319 7 significance significance NN 10_1101-2021_01_08_425864 319 8 in in IN 10_1101-2021_01_08_425864 319 9 this this DT 10_1101-2021_01_08_425864 319 10 figure figure NN 10_1101-2021_01_08_425864 319 11 as as IN 10_1101-2021_01_08_425864 319 12 follows follow VBZ 10_1101-2021_01_08_425864 319 13 : : : 10_1101-2021_01_08_425864 319 14 * * NFP 10_1101-2021_01_08_425864 319 15 * * NFP 10_1101-2021_01_08_425864 319 16 * * NFP 10_1101-2021_01_08_425864 319 17 p p NN 10_1101-2021_01_08_425864 319 18 value value NN 10_1101-2021_01_08_425864 319 19 < < XX 10_1101-2021_01_08_425864 319 20 499 499 CD 10_1101-2021_01_08_425864 319 21 0.001 0.001 CD 10_1101-2021_01_08_425864 319 22 ; ; : 10_1101-2021_01_08_425864 319 23 * * NFP 10_1101-2021_01_08_425864 319 24 * * NFP 10_1101-2021_01_08_425864 319 25 p p NN 10_1101-2021_01_08_425864 319 26 value value NN 10_1101-2021_01_08_425864 319 27 < < XX 10_1101-2021_01_08_425864 319 28 0.01 0.01 XX 10_1101-2021_01_08_425864 319 29 ; ; : 10_1101-2021_01_08_425864 319 30 and and CC 10_1101-2021_01_08_425864 319 31 * * NFP 10_1101-2021_01_08_425864 319 32 p p NN 10_1101-2021_01_08_425864 319 33 value value NN 10_1101-2021_01_08_425864 319 34 < < XX 10_1101-2021_01_08_425864 319 35 0.05 0.05 CD 10_1101-2021_01_08_425864 319 36 . . . 10_1101-2021_01_08_425864 320 1 For for IN 10_1101-2021_01_08_425864 320 2 p p NN 10_1101-2021_01_08_425864 320 3 values value NNS 10_1101-2021_01_08_425864 320 4 > > XX 10_1101-2021_01_08_425864 320 5 0.05 0.05 CD 10_1101-2021_01_08_425864 320 6 , , , 10_1101-2021_01_08_425864 320 7 we -PRON- PRP 10_1101-2021_01_08_425864 320 8 consider consider VBP 10_1101-2021_01_08_425864 320 9 the the DT 10_1101-2021_01_08_425864 320 10 differences difference NNS 10_1101-2021_01_08_425864 320 11 not not RB 10_1101-2021_01_08_425864 320 12 500 500 CD 10_1101-2021_01_08_425864 320 13 significant significant JJ 10_1101-2021_01_08_425864 320 14 ( ( -LRB- 10_1101-2021_01_08_425864 320 15 ns ns NNP 10_1101-2021_01_08_425864 320 16 ) ) -RRB- 10_1101-2021_01_08_425864 320 17 . . . 10_1101-2021_01_08_425864 321 1 501 501 CD 10_1101-2021_01_08_425864 321 2 502 502 CD 10_1101-2021_01_08_425864 321 3 Inhibition inhibition NN 10_1101-2021_01_08_425864 321 4 of of IN 10_1101-2021_01_08_425864 321 5 FAO FAO NNP 10_1101-2021_01_08_425864 321 6 by by IN 10_1101-2021_01_08_425864 321 7 ETO ETO NNP 10_1101-2021_01_08_425864 321 8 affects affect VBZ 10_1101-2021_01_08_425864 321 9 energy energy NN 10_1101-2021_01_08_425864 321 10 metabolism metabolism NN 10_1101-2021_01_08_425864 321 11 , , , 10_1101-2021_01_08_425864 321 12 impairing impair VBG 10_1101-2021_01_08_425864 321 13 the the DT 10_1101-2021_01_08_425864 321 14 consumption consumption NN 10_1101-2021_01_08_425864 321 15 of of IN 10_1101-2021_01_08_425864 321 16 503 503 CD 10_1101-2021_01_08_425864 321 17 endogenous endogenous JJ 10_1101-2021_01_08_425864 321 18 fatty fatty NN 10_1101-2021_01_08_425864 321 19 acids acid NNS 10_1101-2021_01_08_425864 321 20 504 504 CD 10_1101-2021_01_08_425864 321 21 The the DT 10_1101-2021_01_08_425864 321 22 evidence evidence NN 10_1101-2021_01_08_425864 321 23 obtained obtain VBN 10_1101-2021_01_08_425864 321 24 to to IN 10_1101-2021_01_08_425864 321 25 date date NN 10_1101-2021_01_08_425864 321 26 suggests suggest VBZ 10_1101-2021_01_08_425864 321 27 that that IN 10_1101-2021_01_08_425864 321 28 parasites parasite NNS 10_1101-2021_01_08_425864 321 29 resist resist VBP 10_1101-2021_01_08_425864 321 30 metabolic metabolic JJ 10_1101-2021_01_08_425864 321 31 stress stress NN 10_1101-2021_01_08_425864 321 32 by by IN 10_1101-2021_01_08_425864 321 33 mobilizing mobilize VBG 10_1101-2021_01_08_425864 321 34 and and CC 10_1101-2021_01_08_425864 321 35 505 505 CD 10_1101-2021_01_08_425864 321 36 consuming consume VBG 10_1101-2021_01_08_425864 321 37 stored store VBN 10_1101-2021_01_08_425864 321 38 fatty fatty NN 10_1101-2021_01_08_425864 321 39 acids acid NNS 10_1101-2021_01_08_425864 321 40 . . . 10_1101-2021_01_08_425864 322 1 Therefore therefore RB 10_1101-2021_01_08_425864 322 2 , , , 10_1101-2021_01_08_425864 322 3 it -PRON- PRP 10_1101-2021_01_08_425864 322 4 is be VBZ 10_1101-2021_01_08_425864 322 5 reasonable reasonable JJ 10_1101-2021_01_08_425864 322 6 to to TO 10_1101-2021_01_08_425864 322 7 hypothesize hypothesize VB 10_1101-2021_01_08_425864 322 8 that that DT 10_1101-2021_01_08_425864 322 9 ETO ETO NNP 10_1101-2021_01_08_425864 322 10 , , , 10_1101-2021_01_08_425864 322 11 which which WDT 10_1101-2021_01_08_425864 322 12 blocks block VBZ 10_1101-2021_01_08_425864 322 13 the the DT 10_1101-2021_01_08_425864 322 14 506 506 CD 10_1101-2021_01_08_425864 322 15 mobilization mobilization NN 10_1101-2021_01_08_425864 322 16 of of IN 10_1101-2021_01_08_425864 322 17 fatty fatty NN 10_1101-2021_01_08_425864 322 18 acids acid NNS 10_1101-2021_01_08_425864 322 19 into into IN 10_1101-2021_01_08_425864 322 20 the the DT 10_1101-2021_01_08_425864 322 21 mitochondria mitochondria NN 10_1101-2021_01_08_425864 322 22 for for IN 10_1101-2021_01_08_425864 322 23 oxidation oxidation NN 10_1101-2021_01_08_425864 322 24 , , , 10_1101-2021_01_08_425864 322 25 probably probably RB 10_1101-2021_01_08_425864 322 26 perturbs perturb VBZ 10_1101-2021_01_08_425864 322 27 the the DT 10_1101-2021_01_08_425864 322 28 ATP ATP NNP 10_1101-2021_01_08_425864 322 29 levels level NNS 10_1101-2021_01_08_425864 322 30 in in IN 10_1101-2021_01_08_425864 322 31 .CC .CC NFP 10_1101-2021_01_08_425864 322 32 - - : 10_1101-2021_01_08_425864 322 33 BY by IN 10_1101-2021_01_08_425864 322 34 4.0 4.0 CD 10_1101-2021_01_08_425864 322 35 International international JJ 10_1101-2021_01_08_425864 322 36 licensemade licensemade NN 10_1101-2021_01_08_425864 322 37 available available JJ 10_1101-2021_01_08_425864 322 38 under under IN 10_1101-2021_01_08_425864 322 39 a a DT 10_1101-2021_01_08_425864 322 40 ( ( -LRB- 10_1101-2021_01_08_425864 322 41 which which WDT 10_1101-2021_01_08_425864 322 42 was be VBD 10_1101-2021_01_08_425864 322 43 not not RB 10_1101-2021_01_08_425864 322 44 certified certify VBN 10_1101-2021_01_08_425864 322 45 by by IN 10_1101-2021_01_08_425864 322 46 peer peer NN 10_1101-2021_01_08_425864 322 47 review review NN 10_1101-2021_01_08_425864 322 48 ) ) -RRB- 10_1101-2021_01_08_425864 322 49 is be VBZ 10_1101-2021_01_08_425864 322 50 the the DT 10_1101-2021_01_08_425864 322 51 author author NN 10_1101-2021_01_08_425864 322 52 / / SYM 10_1101-2021_01_08_425864 322 53 funder funder NN 10_1101-2021_01_08_425864 322 54 , , , 10_1101-2021_01_08_425864 322 55 who who WP 10_1101-2021_01_08_425864 322 56 has have VBZ 10_1101-2021_01_08_425864 322 57 granted grant VBN 10_1101-2021_01_08_425864 322 58 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 322 59 a a DT 10_1101-2021_01_08_425864 322 60 license license NN 10_1101-2021_01_08_425864 322 61 to to TO 10_1101-2021_01_08_425864 322 62 display display VB 10_1101-2021_01_08_425864 322 63 the the DT 10_1101-2021_01_08_425864 322 64 preprint preprint NN 10_1101-2021_01_08_425864 322 65 in in IN 10_1101-2021_01_08_425864 322 66 perpetuity perpetuity NN 10_1101-2021_01_08_425864 322 67 . . . 10_1101-2021_01_08_425864 323 1 It -PRON- PRP 10_1101-2021_01_08_425864 323 2 is be VBZ 10_1101-2021_01_08_425864 323 3 The the DT 10_1101-2021_01_08_425864 323 4 copyright copyright NN 10_1101-2021_01_08_425864 323 5 holder holder NN 10_1101-2021_01_08_425864 323 6 for for IN 10_1101-2021_01_08_425864 323 7 this this DT 10_1101-2021_01_08_425864 323 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 323 9 version version NN 10_1101-2021_01_08_425864 323 10 posted post VBD 10_1101-2021_01_08_425864 323 11 January January NNP 10_1101-2021_01_08_425864 323 12 8 8 CD 10_1101-2021_01_08_425864 323 13 , , , 10_1101-2021_01_08_425864 323 14 2021 2021 CD 10_1101-2021_01_08_425864 323 15 . . . 10_1101-2021_01_08_425864 323 16 ; ; : 10_1101-2021_01_08_425864 323 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 323 18 : : : 10_1101-2021_01_08_425864 323 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 323 20 preprint preprint NN 10_1101-2021_01_08_425864 323 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 323 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 323 23 507 507 CD 10_1101-2021_01_08_425864 323 24 late late JJ 10_1101-2021_01_08_425864 323 25 - - HYPH 10_1101-2021_01_08_425864 323 26 exponential exponential JJ 10_1101-2021_01_08_425864 323 27 or or CC 10_1101-2021_01_08_425864 323 28 stationary stationary JJ 10_1101-2021_01_08_425864 323 29 phase phase NN 10_1101-2021_01_08_425864 323 30 cells cell NNS 10_1101-2021_01_08_425864 323 31 . . . 10_1101-2021_01_08_425864 324 1 Parasites parasite NNS 10_1101-2021_01_08_425864 324 2 growing grow VBG 10_1101-2021_01_08_425864 324 3 for for IN 10_1101-2021_01_08_425864 324 4 5 5 CD 10_1101-2021_01_08_425864 324 5 days day NNS 10_1101-2021_01_08_425864 324 6 under under IN 10_1101-2021_01_08_425864 324 7 500 500 CD 10_1101-2021_01_08_425864 324 8 µM µM NNP 10_1101-2021_01_08_425864 324 9 ETO eto NN 10_1101-2021_01_08_425864 324 10 treatment treatment NN 10_1101-2021_01_08_425864 324 11 508 508 CD 10_1101-2021_01_08_425864 324 12 or or CC 10_1101-2021_01_08_425864 324 13 no no DT 10_1101-2021_01_08_425864 324 14 treatment treatment NN 10_1101-2021_01_08_425864 324 15 were be VBD 10_1101-2021_01_08_425864 324 16 collected collect VBN 10_1101-2021_01_08_425864 324 17 to to TO 10_1101-2021_01_08_425864 324 18 evaluate evaluate VB 10_1101-2021_01_08_425864 324 19 the the DT 10_1101-2021_01_08_425864 324 20 ability ability NN 10_1101-2021_01_08_425864 324 21 of of IN 10_1101-2021_01_08_425864 324 22 parasites parasite NNS 10_1101-2021_01_08_425864 324 23 that that WDT 10_1101-2021_01_08_425864 324 24 were be VBD 10_1101-2021_01_08_425864 324 25 treated treat VBN 10_1101-2021_01_08_425864 324 26 or or CC 10_1101-2021_01_08_425864 324 27 not not RB 10_1101-2021_01_08_425864 324 28 with with IN 10_1101-2021_01_08_425864 324 29 ETO ETO NNP 10_1101-2021_01_08_425864 324 30 509 509 CD 10_1101-2021_01_08_425864 324 31 to to TO 10_1101-2021_01_08_425864 324 32 trigger trigger VB 10_1101-2021_01_08_425864 324 33 oxygen oxygen NN 10_1101-2021_01_08_425864 324 34 consumption consumption NN 10_1101-2021_01_08_425864 324 35 . . . 10_1101-2021_01_08_425864 325 1 The the DT 10_1101-2021_01_08_425864 325 2 rates rate NNS 10_1101-2021_01_08_425864 325 3 of of IN 10_1101-2021_01_08_425864 325 4 O2 o2 NN 10_1101-2021_01_08_425864 325 5 consumption consumption NN 10_1101-2021_01_08_425864 325 6 corresponding correspond VBG 10_1101-2021_01_08_425864 325 7 to to IN 10_1101-2021_01_08_425864 325 8 basal basal NN 10_1101-2021_01_08_425864 325 9 respiration respiration NN 10_1101-2021_01_08_425864 325 10 were be VBD 10_1101-2021_01_08_425864 325 11 510 510 CD 10_1101-2021_01_08_425864 325 12 measured measure VBN 10_1101-2021_01_08_425864 325 13 in in IN 10_1101-2021_01_08_425864 325 14 cells cell NNS 10_1101-2021_01_08_425864 325 15 resuspended resuspend VBN 10_1101-2021_01_08_425864 325 16 in in IN 10_1101-2021_01_08_425864 325 17 MCR MCR NNP 10_1101-2021_01_08_425864 325 18 respiration respiration NN 10_1101-2021_01_08_425864 325 19 buffer buffer NN 10_1101-2021_01_08_425864 325 20 . . . 10_1101-2021_01_08_425864 326 1 We -PRON- PRP 10_1101-2021_01_08_425864 326 2 then then RB 10_1101-2021_01_08_425864 326 3 measured measure VBD 10_1101-2021_01_08_425864 326 4 the the DT 10_1101-2021_01_08_425864 326 5 leak leak NN 10_1101-2021_01_08_425864 326 6 respiration respiration NN 10_1101-2021_01_08_425864 326 7 by by IN 10_1101-2021_01_08_425864 326 8 511 511 CD 10_1101-2021_01_08_425864 326 9 inhibiting inhibit VBG 10_1101-2021_01_08_425864 326 10 the the DT 10_1101-2021_01_08_425864 326 11 ATP ATP NNP 10_1101-2021_01_08_425864 326 12 synthase synthase NN 10_1101-2021_01_08_425864 326 13 with with IN 10_1101-2021_01_08_425864 326 14 oligomycin oligomycin NNP 10_1101-2021_01_08_425864 326 15 A. a. NN 10_1101-2021_01_08_425864 327 1 Finally finally RB 10_1101-2021_01_08_425864 327 2 , , , 10_1101-2021_01_08_425864 327 3 to to TO 10_1101-2021_01_08_425864 327 4 measure measure VB 10_1101-2021_01_08_425864 327 5 the the DT 10_1101-2021_01_08_425864 327 6 maximum maximum JJ 10_1101-2021_01_08_425864 327 7 capacity capacity NN 10_1101-2021_01_08_425864 327 8 of of IN 10_1101-2021_01_08_425864 327 9 the the DT 10_1101-2021_01_08_425864 327 10 512 512 CD 10_1101-2021_01_08_425864 327 11 electron electron NN 10_1101-2021_01_08_425864 327 12 transport transport NN 10_1101-2021_01_08_425864 327 13 system system NN 10_1101-2021_01_08_425864 327 14 ( ( -LRB- 10_1101-2021_01_08_425864 327 15 ETS ETS NNP 10_1101-2021_01_08_425864 327 16 ) ) -RRB- 10_1101-2021_01_08_425864 327 17 , , , 10_1101-2021_01_08_425864 327 18 we -PRON- PRP 10_1101-2021_01_08_425864 327 19 used use VBD 10_1101-2021_01_08_425864 327 20 the the DT 10_1101-2021_01_08_425864 327 21 uncoupler uncoupler NN 10_1101-2021_01_08_425864 327 22 FCCP FCCP NNP 10_1101-2021_01_08_425864 327 23 [ [ -LRB- 10_1101-2021_01_08_425864 327 24 21 21 CD 10_1101-2021_01_08_425864 327 25 ] ] -RRB- 10_1101-2021_01_08_425864 327 26 . . . 10_1101-2021_01_08_425864 328 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 328 2 results result NNS 10_1101-2021_01_08_425864 328 3 demonstrate demonstrate VBP 10_1101-2021_01_08_425864 328 4 that that IN 10_1101-2021_01_08_425864 328 5 513 513 CD 10_1101-2021_01_08_425864 328 6 compared compare VBN 10_1101-2021_01_08_425864 328 7 to to IN 10_1101-2021_01_08_425864 328 8 no no DT 10_1101-2021_01_08_425864 328 9 treatment treatment NN 10_1101-2021_01_08_425864 328 10 , , , 10_1101-2021_01_08_425864 328 11 ETO eto NN 10_1101-2021_01_08_425864 328 12 treatment treatment NN 10_1101-2021_01_08_425864 328 13 diminishes diminish VBZ 10_1101-2021_01_08_425864 328 14 the the DT 10_1101-2021_01_08_425864 328 15 rate rate NN 10_1101-2021_01_08_425864 328 16 of of IN 10_1101-2021_01_08_425864 328 17 basal basal NNP 10_1101-2021_01_08_425864 328 18 oxygen oxygen NNP 10_1101-2021_01_08_425864 328 19 consumption consumption NN 10_1101-2021_01_08_425864 328 20 , , , 10_1101-2021_01_08_425864 328 21 the the DT 10_1101-2021_01_08_425864 328 22 leak leak NN 10_1101-2021_01_08_425864 328 23 514 514 CD 10_1101-2021_01_08_425864 328 24 respiration respiration NN 10_1101-2021_01_08_425864 328 25 and and CC 10_1101-2021_01_08_425864 328 26 the the DT 10_1101-2021_01_08_425864 328 27 ETS ETS NNP 10_1101-2021_01_08_425864 328 28 capacity capacity NN 10_1101-2021_01_08_425864 328 29 . . . 10_1101-2021_01_08_425864 329 1 In in IN 10_1101-2021_01_08_425864 329 2 general general JJ 10_1101-2021_01_08_425864 329 3 , , , 10_1101-2021_01_08_425864 329 4 respiratory respiratory JJ 10_1101-2021_01_08_425864 329 5 rates rate NNS 10_1101-2021_01_08_425864 329 6 diminished diminish VBN 10_1101-2021_01_08_425864 329 7 in in IN 10_1101-2021_01_08_425864 329 8 parasites parasite NNS 10_1101-2021_01_08_425864 329 9 treated treat VBN 10_1101-2021_01_08_425864 329 10 with with IN 10_1101-2021_01_08_425864 329 11 515 515 CD 10_1101-2021_01_08_425864 329 12 ETO eto NN 10_1101-2021_01_08_425864 329 13 when when WRB 10_1101-2021_01_08_425864 329 14 compared compare VBN 10_1101-2021_01_08_425864 329 15 to to IN 10_1101-2021_01_08_425864 329 16 the the DT 10_1101-2021_01_08_425864 329 17 untreated untreated JJ 10_1101-2021_01_08_425864 329 18 ones one NNS 10_1101-2021_01_08_425864 329 19 . . . 10_1101-2021_01_08_425864 330 1 As as IN 10_1101-2021_01_08_425864 330 2 expected expect VBN 10_1101-2021_01_08_425864 330 3 , , , 10_1101-2021_01_08_425864 330 4 ETO ETO NNP 10_1101-2021_01_08_425864 330 5 treatment treatment NN 10_1101-2021_01_08_425864 330 6 led lead VBD 10_1101-2021_01_08_425864 330 7 to to IN 10_1101-2021_01_08_425864 330 8 a a DT 10_1101-2021_01_08_425864 330 9 75 75 CD 10_1101-2021_01_08_425864 330 10 % % NN 10_1101-2021_01_08_425864 330 11 decrease decrease NN 10_1101-2021_01_08_425864 330 12 in in IN 10_1101-2021_01_08_425864 330 13 the the DT 10_1101-2021_01_08_425864 330 14 516 516 CD 10_1101-2021_01_08_425864 330 15 levels level NNS 10_1101-2021_01_08_425864 330 16 of of IN 10_1101-2021_01_08_425864 330 17 total total JJ 10_1101-2021_01_08_425864 330 18 intracellular intracellular JJ 10_1101-2021_01_08_425864 330 19 ATP ATP NNP 10_1101-2021_01_08_425864 330 20 compared compare VBN 10_1101-2021_01_08_425864 330 21 to to IN 10_1101-2021_01_08_425864 330 22 untreated untreated JJ 10_1101-2021_01_08_425864 330 23 parasites parasite NNS 10_1101-2021_01_08_425864 330 24 ( ( -LRB- 10_1101-2021_01_08_425864 330 25 Fig Fig NNP 10_1101-2021_01_08_425864 330 26 10A 10A NNP 10_1101-2021_01_08_425864 330 27 ) ) -RRB- 10_1101-2021_01_08_425864 330 28 . . . 10_1101-2021_01_08_425864 331 1 To to TO 10_1101-2021_01_08_425864 331 2 complement complement VB 10_1101-2021_01_08_425864 331 3 this this DT 10_1101-2021_01_08_425864 331 4 517 517 CD 10_1101-2021_01_08_425864 331 5 result result NN 10_1101-2021_01_08_425864 331 6 , , , 10_1101-2021_01_08_425864 331 7 because because IN 10_1101-2021_01_08_425864 331 8 all all PDT 10_1101-2021_01_08_425864 331 9 these these DT 10_1101-2021_01_08_425864 331 10 experiments experiment NNS 10_1101-2021_01_08_425864 331 11 were be VBD 10_1101-2021_01_08_425864 331 12 conducted conduct VBN 10_1101-2021_01_08_425864 331 13 in in IN 10_1101-2021_01_08_425864 331 14 the the DT 10_1101-2021_01_08_425864 331 15 complete complete JJ 10_1101-2021_01_08_425864 331 16 absence absence NN 10_1101-2021_01_08_425864 331 17 of of IN 10_1101-2021_01_08_425864 331 18 an an DT 10_1101-2021_01_08_425864 331 19 oxidizable oxidizable JJ 10_1101-2021_01_08_425864 331 20 518 518 CD 10_1101-2021_01_08_425864 331 21 external external JJ 10_1101-2021_01_08_425864 331 22 metabolite metabolite NN 10_1101-2021_01_08_425864 331 23 , , , 10_1101-2021_01_08_425864 331 24 our -PRON- PRP$ 10_1101-2021_01_08_425864 331 25 results result NNS 10_1101-2021_01_08_425864 331 26 show show VBP 10_1101-2021_01_08_425864 331 27 that that IN 10_1101-2021_01_08_425864 331 28 the the DT 10_1101-2021_01_08_425864 331 29 parasite parasite NN 10_1101-2021_01_08_425864 331 30 is be VBZ 10_1101-2021_01_08_425864 331 31 able able JJ 10_1101-2021_01_08_425864 331 32 to to TO 10_1101-2021_01_08_425864 331 33 oxidize oxidize VB 10_1101-2021_01_08_425864 331 34 internal internal JJ 10_1101-2021_01_08_425864 331 35 metabolites metabolite NNS 10_1101-2021_01_08_425864 331 36 ( ( -LRB- 10_1101-2021_01_08_425864 331 37 Figs fig NNS 10_1101-2021_01_08_425864 331 38 10B 10B NNP 10_1101-2021_01_08_425864 331 39 519 519 CD 10_1101-2021_01_08_425864 331 40 and and CC 10_1101-2021_01_08_425864 331 41 10C 10c CD 10_1101-2021_01_08_425864 331 42 ) ) -RRB- 10_1101-2021_01_08_425864 331 43 . . . 10_1101-2021_01_08_425864 332 1 Taking take VBG 10_1101-2021_01_08_425864 332 2 into into IN 10_1101-2021_01_08_425864 332 3 account account NN 10_1101-2021_01_08_425864 332 4 that that IN 10_1101-2021_01_08_425864 332 5 treating treat VBG 10_1101-2021_01_08_425864 332 6 parasites parasite NNS 10_1101-2021_01_08_425864 332 7 with with IN 10_1101-2021_01_08_425864 332 8 ETO ETO NNP 10_1101-2021_01_08_425864 332 9 diminished diminish VBD 10_1101-2021_01_08_425864 332 10 the the DT 10_1101-2021_01_08_425864 332 11 basal basal NN 10_1101-2021_01_08_425864 332 12 respiration respiration NN 10_1101-2021_01_08_425864 332 13 rates rate NNS 10_1101-2021_01_08_425864 332 14 520 520 CD 10_1101-2021_01_08_425864 332 15 of of IN 10_1101-2021_01_08_425864 332 16 these these DT 10_1101-2021_01_08_425864 332 17 parasites parasite NNS 10_1101-2021_01_08_425864 332 18 by by IN 10_1101-2021_01_08_425864 332 19 approximately approximately RB 10_1101-2021_01_08_425864 332 20 one one CD 10_1101-2021_01_08_425864 332 21 - - HYPH 10_1101-2021_01_08_425864 332 22 half half NN 10_1101-2021_01_08_425864 332 23 ( ( -LRB- 10_1101-2021_01_08_425864 332 24 Figs fig NNS 10_1101-2021_01_08_425864 332 25 10B 10B NNP 10_1101-2021_01_08_425864 332 26 and and CC 10_1101-2021_01_08_425864 332 27 10C 10c CD 10_1101-2021_01_08_425864 332 28 ) ) -RRB- 10_1101-2021_01_08_425864 332 29 , , , 10_1101-2021_01_08_425864 332 30 it -PRON- PRP 10_1101-2021_01_08_425864 332 31 is be VBZ 10_1101-2021_01_08_425864 332 32 reasonable reasonable JJ 10_1101-2021_01_08_425864 332 33 to to TO 10_1101-2021_01_08_425864 332 34 conclude conclude VB 10_1101-2021_01_08_425864 332 35 that that IN 10_1101-2021_01_08_425864 332 36 a a DT 10_1101-2021_01_08_425864 332 37 521 521 CD 10_1101-2021_01_08_425864 332 38 relevant relevant JJ 10_1101-2021_01_08_425864 332 39 part part NN 10_1101-2021_01_08_425864 332 40 of of IN 10_1101-2021_01_08_425864 332 41 the the DT 10_1101-2021_01_08_425864 332 42 respiration respiration NN 10_1101-2021_01_08_425864 332 43 in in IN 10_1101-2021_01_08_425864 332 44 the the DT 10_1101-2021_01_08_425864 332 45 absence absence NN 10_1101-2021_01_08_425864 332 46 of of IN 10_1101-2021_01_08_425864 332 47 external external JJ 10_1101-2021_01_08_425864 332 48 oxidisable oxidisable JJ 10_1101-2021_01_08_425864 332 49 metabolites metabolite NNS 10_1101-2021_01_08_425864 332 50 is be VBZ 10_1101-2021_01_08_425864 332 51 based base VBN 10_1101-2021_01_08_425864 332 52 on on IN 10_1101-2021_01_08_425864 332 53 the the DT 10_1101-2021_01_08_425864 332 54 522 522 CD 10_1101-2021_01_08_425864 332 55 consumption consumption NN 10_1101-2021_01_08_425864 332 56 of of IN 10_1101-2021_01_08_425864 332 57 internal internal JJ 10_1101-2021_01_08_425864 332 58 lipids lipid NNS 10_1101-2021_01_08_425864 332 59 . . . 10_1101-2021_01_08_425864 333 1 This this DT 10_1101-2021_01_08_425864 333 2 is be VBZ 10_1101-2021_01_08_425864 333 3 consistent consistent JJ 10_1101-2021_01_08_425864 333 4 with with IN 10_1101-2021_01_08_425864 333 5 the the DT 10_1101-2021_01_08_425864 333 6 confirmation confirmation NN 10_1101-2021_01_08_425864 333 7 that that WDT 10_1101-2021_01_08_425864 333 8 epimastigotes epimastigote VBZ 10_1101-2021_01_08_425864 333 9 maintain maintain VBP 10_1101-2021_01_08_425864 333 10 523 523 CD 10_1101-2021_01_08_425864 333 11 their -PRON- PRP$ 10_1101-2021_01_08_425864 333 12 viability viability NN 10_1101-2021_01_08_425864 333 13 in in IN 10_1101-2021_01_08_425864 333 14 the the DT 10_1101-2021_01_08_425864 333 15 presence presence NN 10_1101-2021_01_08_425864 333 16 of of IN 10_1101-2021_01_08_425864 333 17 non non JJ 10_1101-2021_01_08_425864 333 18 - - JJ 10_1101-2021_01_08_425864 333 19 fatty fatty JJ 10_1101-2021_01_08_425864 333 20 acid acid NN 10_1101-2021_01_08_425864 333 21 carbon carbon NN 10_1101-2021_01_08_425864 333 22 sources source NNS 10_1101-2021_01_08_425864 333 23 in in IN 10_1101-2021_01_08_425864 333 24 the the DT 10_1101-2021_01_08_425864 333 25 presence presence NN 10_1101-2021_01_08_425864 333 26 of of IN 10_1101-2021_01_08_425864 333 27 ETO ETO NNP 10_1101-2021_01_08_425864 333 28 ( ( -LRB- 10_1101-2021_01_08_425864 333 29 S4 S4 NNP 10_1101-2021_01_08_425864 333 30 Fig Fig NNP 10_1101-2021_01_08_425864 333 31 ) ) -RRB- 10_1101-2021_01_08_425864 333 32 . . . 10_1101-2021_01_08_425864 334 1 In in IN 10_1101-2021_01_08_425864 334 2 524 524 CD 10_1101-2021_01_08_425864 334 3 summary summary NN 10_1101-2021_01_08_425864 334 4 , , , 10_1101-2021_01_08_425864 334 5 these these DT 10_1101-2021_01_08_425864 334 6 results result NNS 10_1101-2021_01_08_425864 334 7 confirm confirm VBP 10_1101-2021_01_08_425864 334 8 that that IN 10_1101-2021_01_08_425864 334 9 ETO ETO NNP 10_1101-2021_01_08_425864 334 10 is be VBZ 10_1101-2021_01_08_425864 334 11 interfering interfere VBG 10_1101-2021_01_08_425864 334 12 with with IN 10_1101-2021_01_08_425864 334 13 ATP ATP NNP 10_1101-2021_01_08_425864 334 14 synthesis synthesis NN 10_1101-2021_01_08_425864 334 15 through through IN 10_1101-2021_01_08_425864 334 16 oxidative oxidative JJ 10_1101-2021_01_08_425864 334 17 525 525 CD 10_1101-2021_01_08_425864 334 18 phosphorylation phosphorylation NN 10_1101-2021_01_08_425864 334 19 in in IN 10_1101-2021_01_08_425864 334 20 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 334 21 forms form NNS 10_1101-2021_01_08_425864 334 22 . . . 10_1101-2021_01_08_425864 335 1 526 526 CD 10_1101-2021_01_08_425864 335 2 527 527 CD 10_1101-2021_01_08_425864 335 3 Figure figure NN 10_1101-2021_01_08_425864 335 4 10 10 CD 10_1101-2021_01_08_425864 335 5 . . . 10_1101-2021_01_08_425864 336 1 Effects effect NNS 10_1101-2021_01_08_425864 336 2 of of IN 10_1101-2021_01_08_425864 336 3 ETO eto NN 10_1101-2021_01_08_425864 336 4 on on IN 10_1101-2021_01_08_425864 336 5 respiration respiration NN 10_1101-2021_01_08_425864 336 6 and and CC 10_1101-2021_01_08_425864 336 7 ATP atp NN 10_1101-2021_01_08_425864 336 8 production production NN 10_1101-2021_01_08_425864 336 9 in in IN 10_1101-2021_01_08_425864 336 10 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 336 11 forms form NNS 10_1101-2021_01_08_425864 336 12 of of IN 10_1101-2021_01_08_425864 336 13 T. T. NNP 10_1101-2021_01_08_425864 336 14 cruzi cruzi NNS 10_1101-2021_01_08_425864 336 15 . . . 10_1101-2021_01_08_425864 337 1 528 528 CD 10_1101-2021_01_08_425864 337 2 ( ( -LRB- 10_1101-2021_01_08_425864 337 3 A a DT 10_1101-2021_01_08_425864 337 4 ) ) -RRB- 10_1101-2021_01_08_425864 337 5 Oxygen oxygen NN 10_1101-2021_01_08_425864 337 6 consumption consumption NN 10_1101-2021_01_08_425864 337 7 of of IN 10_1101-2021_01_08_425864 337 8 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 337 9 forms form NNS 10_1101-2021_01_08_425864 337 10 after after IN 10_1101-2021_01_08_425864 337 11 normal normal JJ 10_1101-2021_01_08_425864 337 12 growth growth NN 10_1101-2021_01_08_425864 337 13 in in IN 10_1101-2021_01_08_425864 337 14 LIT LIT NNP 10_1101-2021_01_08_425864 337 15 medium medium NN 10_1101-2021_01_08_425864 337 16 . . . 10_1101-2021_01_08_425864 338 1 ( ( -LRB- 10_1101-2021_01_08_425864 338 2 B b NN 10_1101-2021_01_08_425864 338 3 ) ) -RRB- 10_1101-2021_01_08_425864 338 4 Oxygen Oxygen NNP 10_1101-2021_01_08_425864 338 5 529 529 CD 10_1101-2021_01_08_425864 338 6 consumption consumption NN 10_1101-2021_01_08_425864 338 7 after after IN 10_1101-2021_01_08_425864 338 8 ETO ETO NNP 10_1101-2021_01_08_425864 338 9 500 500 CD 10_1101-2021_01_08_425864 338 10 μM μM NNP 10_1101-2021_01_08_425864 338 11 treatment treatment NN 10_1101-2021_01_08_425864 338 12 . . . 10_1101-2021_01_08_425864 339 1 Parasite parasite VB 10_1101-2021_01_08_425864 339 2 growth growth NN 10_1101-2021_01_08_425864 339 3 in in IN 10_1101-2021_01_08_425864 339 4 LIT LIT NNP 10_1101-2021_01_08_425864 339 5 medium medium NN 10_1101-2021_01_08_425864 339 6 with with IN 10_1101-2021_01_08_425864 339 7 the the DT 10_1101-2021_01_08_425864 339 8 compound compound NN 10_1101-2021_01_08_425864 339 9 until until IN 10_1101-2021_01_08_425864 339 10 530 530 CD 10_1101-2021_01_08_425864 339 11 the the DT 10_1101-2021_01_08_425864 339 12 5th 5th JJ 10_1101-2021_01_08_425864 339 13 day day NN 10_1101-2021_01_08_425864 339 14 . . . 10_1101-2021_01_08_425864 340 1 In in IN 10_1101-2021_01_08_425864 340 2 black black JJ 10_1101-2021_01_08_425864 340 3 , , , 10_1101-2021_01_08_425864 340 4 a a DT 10_1101-2021_01_08_425864 340 5 time time NN 10_1101-2021_01_08_425864 340 6 - - HYPH 10_1101-2021_01_08_425864 340 7 course course NN 10_1101-2021_01_08_425864 340 8 register register NN 10_1101-2021_01_08_425864 340 9 of of IN 10_1101-2021_01_08_425864 340 10 the the DT 10_1101-2021_01_08_425864 340 11 concentration concentration NN 10_1101-2021_01_08_425864 340 12 ( ( -LRB- 10_1101-2021_01_08_425864 340 13 pmols pmols NNP 10_1101-2021_01_08_425864 340 14 ) ) -RRB- 10_1101-2021_01_08_425864 340 15 of of IN 10_1101-2021_01_08_425864 340 16 O2 o2 NN 10_1101-2021_01_08_425864 340 17 in in IN 10_1101-2021_01_08_425864 340 18 the the DT 10_1101-2021_01_08_425864 340 19 respiration respiration NN 10_1101-2021_01_08_425864 340 20 531 531 CD 10_1101-2021_01_08_425864 340 21 chamber chamber NN 10_1101-2021_01_08_425864 340 22 . . . 10_1101-2021_01_08_425864 341 1 In in IN 10_1101-2021_01_08_425864 341 2 blue blue JJ 10_1101-2021_01_08_425864 341 3 , , , 10_1101-2021_01_08_425864 341 4 negative negative JJ 10_1101-2021_01_08_425864 341 5 of of IN 10_1101-2021_01_08_425864 341 6 the the DT 10_1101-2021_01_08_425864 341 7 concentration concentration NN 10_1101-2021_01_08_425864 341 8 derivative derivative JJ 10_1101-2021_01_08_425864 341 9 ( ( -LRB- 10_1101-2021_01_08_425864 341 10 pmols pmols NNP 10_1101-2021_01_08_425864 341 11 ) ) -RRB- 10_1101-2021_01_08_425864 341 12 of of IN 10_1101-2021_01_08_425864 341 13 O2 o2 NN 10_1101-2021_01_08_425864 341 14 with with IN 10_1101-2021_01_08_425864 341 15 respect respect NN 10_1101-2021_01_08_425864 341 16 to to IN 10_1101-2021_01_08_425864 341 17 time time NN 10_1101-2021_01_08_425864 341 18 ( ( -LRB- 10_1101-2021_01_08_425864 341 19 velocity velocity NN 10_1101-2021_01_08_425864 341 20 532 532 CD 10_1101-2021_01_08_425864 341 21 of of IN 10_1101-2021_01_08_425864 341 22 O2 O2 NNP 10_1101-2021_01_08_425864 341 23 consumption consumption NN 10_1101-2021_01_08_425864 341 24 in in IN 10_1101-2021_01_08_425864 341 25 pmoles pmole NNS 10_1101-2021_01_08_425864 341 26 per per IN 10_1101-2021_01_08_425864 341 27 second second NN 10_1101-2021_01_08_425864 341 28 ) ) -RRB- 10_1101-2021_01_08_425864 341 29 . . . 10_1101-2021_01_08_425864 342 1 The the DT 10_1101-2021_01_08_425864 342 2 parasites parasite NNS 10_1101-2021_01_08_425864 342 3 were be VBD 10_1101-2021_01_08_425864 342 4 washed wash VBN 10_1101-2021_01_08_425864 342 5 twice twice RB 10_1101-2021_01_08_425864 342 6 in in IN 10_1101-2021_01_08_425864 342 7 PBS PBS NNP 10_1101-2021_01_08_425864 342 8 and and CC 10_1101-2021_01_08_425864 342 9 kept keep VBD 10_1101-2021_01_08_425864 342 10 in in IN 10_1101-2021_01_08_425864 342 11 MRC MRC NNP 10_1101-2021_01_08_425864 342 12 533 533 CD 10_1101-2021_01_08_425864 342 13 buffer buffer NN 10_1101-2021_01_08_425864 342 14 at at IN 10_1101-2021_01_08_425864 342 15 28 28 CD 10_1101-2021_01_08_425864 342 16 ° ° , 10_1101-2021_01_08_425864 342 17 C C NNP 10_1101-2021_01_08_425864 342 18 during during IN 10_1101-2021_01_08_425864 342 19 the the DT 10_1101-2021_01_08_425864 342 20 assays assay NNS 10_1101-2021_01_08_425864 342 21 ( ( -LRB- 10_1101-2021_01_08_425864 342 22 see see VB 10_1101-2021_01_08_425864 342 23 Materials material NNS 10_1101-2021_01_08_425864 342 24 and and CC 10_1101-2021_01_08_425864 342 25 Methods Methods NNPS 10_1101-2021_01_08_425864 342 26 for for IN 10_1101-2021_01_08_425864 342 27 more more JJR 10_1101-2021_01_08_425864 342 28 details detail NNS 10_1101-2021_01_08_425864 342 29 ) ) -RRB- 10_1101-2021_01_08_425864 342 30 . . . 10_1101-2021_01_08_425864 343 1 ( ( -LRB- 10_1101-2021_01_08_425864 343 2 C C NNP 10_1101-2021_01_08_425864 343 3 ) ) -RRB- 10_1101-2021_01_08_425864 343 4 The the DT 10_1101-2021_01_08_425864 343 5 basal basal NN 10_1101-2021_01_08_425864 343 6 534 534 CD 10_1101-2021_01_08_425864 343 7 respiration respiration NN 10_1101-2021_01_08_425864 343 8 ( ( -LRB- 10_1101-2021_01_08_425864 343 9 initial initial JJ 10_1101-2021_01_08_425864 343 10 oxygen oxygen NN 10_1101-2021_01_08_425864 343 11 flux flux NN 10_1101-2021_01_08_425864 343 12 values value NNS 10_1101-2021_01_08_425864 343 13 , , , 10_1101-2021_01_08_425864 343 14 MRC MRC NNP 10_1101-2021_01_08_425864 343 15 ) ) -RRB- 10_1101-2021_01_08_425864 343 16 , , , 10_1101-2021_01_08_425864 343 17 respiration respiration NN 10_1101-2021_01_08_425864 343 18 leak leak NN 10_1101-2021_01_08_425864 343 19 after after IN 10_1101-2021_01_08_425864 343 20 the the DT 10_1101-2021_01_08_425864 343 21 sequential sequential JJ 10_1101-2021_01_08_425864 343 22 addition addition NN 10_1101-2021_01_08_425864 343 23 of of IN 10_1101-2021_01_08_425864 343 24 0.5 0.5 CD 10_1101-2021_01_08_425864 343 25 535 535 CD 10_1101-2021_01_08_425864 343 26 µg µg IN 10_1101-2021_01_08_425864 343 27 / / SYM 10_1101-2021_01_08_425864 343 28 mL ml NN 10_1101-2021_01_08_425864 343 29 of of IN 10_1101-2021_01_08_425864 343 30 oligomycin oligomycin NNP 10_1101-2021_01_08_425864 343 31 A a NN 10_1101-2021_01_08_425864 343 32 ( ( -LRB- 10_1101-2021_01_08_425864 343 33 2 2 CD 10_1101-2021_01_08_425864 343 34 µg µg NN 10_1101-2021_01_08_425864 343 35 / / SYM 10_1101-2021_01_08_425864 343 36 mL ml NN 10_1101-2021_01_08_425864 343 37 ) ) -RRB- 10_1101-2021_01_08_425864 343 38 , , , 10_1101-2021_01_08_425864 343 39 and and CC 10_1101-2021_01_08_425864 343 40 electron electron NN 10_1101-2021_01_08_425864 343 41 transfer transfer NN 10_1101-2021_01_08_425864 343 42 system system NN 10_1101-2021_01_08_425864 343 43 ( ( -LRB- 10_1101-2021_01_08_425864 343 44 ETS ETS NNP 10_1101-2021_01_08_425864 343 45 ) ) -RRB- 10_1101-2021_01_08_425864 343 46 capacity capacity NN 10_1101-2021_01_08_425864 343 47 after after IN 10_1101-2021_01_08_425864 343 48 the the DT 10_1101-2021_01_08_425864 343 49 sequential sequential JJ 10_1101-2021_01_08_425864 343 50 536 536 CD 10_1101-2021_01_08_425864 343 51 addition addition NN 10_1101-2021_01_08_425864 343 52 of of IN 10_1101-2021_01_08_425864 343 53 0.5 0.5 CD 10_1101-2021_01_08_425864 343 54 µM µM NNP 10_1101-2021_01_08_425864 343 55 FCCP FCCP NNP 10_1101-2021_01_08_425864 343 56 ( ( -LRB- 10_1101-2021_01_08_425864 343 57 2 2 CD 10_1101-2021_01_08_425864 343 58 µM µM NNP 10_1101-2021_01_08_425864 343 59 ) ) -RRB- 10_1101-2021_01_08_425864 343 60 were be VBD 10_1101-2021_01_08_425864 343 61 measured measure VBN 10_1101-2021_01_08_425864 343 62 for for IN 10_1101-2021_01_08_425864 343 63 each each DT 10_1101-2021_01_08_425864 343 64 condition condition NN 10_1101-2021_01_08_425864 343 65 . . . 10_1101-2021_01_08_425864 344 1 ( ( -LRB- 10_1101-2021_01_08_425864 344 2 D d NN 10_1101-2021_01_08_425864 344 3 ) ) -RRB- 10_1101-2021_01_08_425864 344 4 Intracellular intracellular JJ 10_1101-2021_01_08_425864 344 5 levels level NNS 10_1101-2021_01_08_425864 344 6 of of IN 10_1101-2021_01_08_425864 344 7 ATP ATP NNP 10_1101-2021_01_08_425864 344 8 537 537 CD 10_1101-2021_01_08_425864 344 9 after after IN 10_1101-2021_01_08_425864 344 10 treating treat VBG 10_1101-2021_01_08_425864 344 11 with with IN 10_1101-2021_01_08_425864 344 12 500 500 CD 10_1101-2021_01_08_425864 344 13 µM µM NNP 10_1101-2021_01_08_425864 344 14 ETO ETO NNP 10_1101-2021_01_08_425864 344 15 . . . 10_1101-2021_01_08_425864 345 1 The the DT 10_1101-2021_01_08_425864 345 2 intracellular intracellular JJ 10_1101-2021_01_08_425864 345 3 ATP atp NN 10_1101-2021_01_08_425864 345 4 content content NN 10_1101-2021_01_08_425864 345 5 was be VBD 10_1101-2021_01_08_425864 345 6 assessed assess VBN 10_1101-2021_01_08_425864 345 7 following follow VBG 10_1101-2021_01_08_425864 345 8 incubation incubation NN 10_1101-2021_01_08_425864 345 9 538 538 CD 10_1101-2021_01_08_425864 345 10 with with IN 10_1101-2021_01_08_425864 345 11 different different JJ 10_1101-2021_01_08_425864 345 12 energy energy NN 10_1101-2021_01_08_425864 345 13 substrates substrate NNS 10_1101-2021_01_08_425864 345 14 or or CC 10_1101-2021_01_08_425864 345 15 not not RB 10_1101-2021_01_08_425864 345 16 ( ( -LRB- 10_1101-2021_01_08_425864 345 17 PBS PBS NNP 10_1101-2021_01_08_425864 345 18 , , , 10_1101-2021_01_08_425864 345 19 negative negative JJ 10_1101-2021_01_08_425864 345 20 control control NN 10_1101-2021_01_08_425864 345 21 ) ) -RRB- 10_1101-2021_01_08_425864 345 22 . . . 10_1101-2021_01_08_425864 346 1 The the DT 10_1101-2021_01_08_425864 346 2 ATP ATP NNP 10_1101-2021_01_08_425864 346 3 concentration concentration NN 10_1101-2021_01_08_425864 346 4 was be VBD 10_1101-2021_01_08_425864 346 5 539 539 CD 10_1101-2021_01_08_425864 346 6 determined determine VBN 10_1101-2021_01_08_425864 346 7 by by IN 10_1101-2021_01_08_425864 346 8 luciferase luciferase NN 10_1101-2021_01_08_425864 346 9 assay assay NNP 10_1101-2021_01_08_425864 346 10 and and CC 10_1101-2021_01_08_425864 346 11 the the DT 10_1101-2021_01_08_425864 346 12 data datum NNS 10_1101-2021_01_08_425864 346 13 were be VBD 10_1101-2021_01_08_425864 346 14 adjusted adjust VBN 10_1101-2021_01_08_425864 346 15 by by IN 10_1101-2021_01_08_425864 346 16 the the DT 10_1101-2021_01_08_425864 346 17 number number NN 10_1101-2021_01_08_425864 346 18 of of IN 10_1101-2021_01_08_425864 346 19 cells cell NNS 10_1101-2021_01_08_425864 346 20 . . . 10_1101-2021_01_08_425864 347 1 All all PDT 10_1101-2021_01_08_425864 347 2 the the DT 10_1101-2021_01_08_425864 347 3 experiments experiment NNS 10_1101-2021_01_08_425864 347 4 540 540 CD 10_1101-2021_01_08_425864 347 5 were be VBD 10_1101-2021_01_08_425864 347 6 performed perform VBN 10_1101-2021_01_08_425864 347 7 in in IN 10_1101-2021_01_08_425864 347 8 triplicates triplicate NNS 10_1101-2021_01_08_425864 347 9 . . . 10_1101-2021_01_08_425864 348 1 Statistical statistical JJ 10_1101-2021_01_08_425864 348 2 analysis analysis NN 10_1101-2021_01_08_425864 348 3 was be VBD 10_1101-2021_01_08_425864 348 4 performed perform VBN 10_1101-2021_01_08_425864 348 5 with with IN 10_1101-2021_01_08_425864 348 6 one one CD 10_1101-2021_01_08_425864 348 7 - - HYPH 10_1101-2021_01_08_425864 348 8 way way NN 10_1101-2021_01_08_425864 348 9 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 348 10 followed follow VBN 10_1101-2021_01_08_425864 348 11 by by IN 10_1101-2021_01_08_425864 348 12 541 541 CD 10_1101-2021_01_08_425864 348 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 348 14 's 's POS 10_1101-2021_01_08_425864 348 15 post post JJ 10_1101-2021_01_08_425864 348 16 - - NN 10_1101-2021_01_08_425864 348 17 test test NN 10_1101-2021_01_08_425864 348 18 at at IN 10_1101-2021_01_08_425864 348 19 p p NN 10_1101-2021_01_08_425864 348 20 < < XX 10_1101-2021_01_08_425864 348 21 0.05 0.05 XX 10_1101-2021_01_08_425864 348 22 using use VBG 10_1101-2021_01_08_425864 348 23 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 348 24 Prism Prism NNP 10_1101-2021_01_08_425864 348 25 8.0.2 8.0.2 CD 10_1101-2021_01_08_425864 348 26 software software NN 10_1101-2021_01_08_425864 348 27 . . . 10_1101-2021_01_08_425864 349 1 We -PRON- PRP 10_1101-2021_01_08_425864 349 2 represent represent VBP 10_1101-2021_01_08_425864 349 3 the the DT 10_1101-2021_01_08_425864 349 4 level level NN 10_1101-2021_01_08_425864 349 5 of of IN 10_1101-2021_01_08_425864 349 6 statistical statistical JJ 10_1101-2021_01_08_425864 349 7 542 542 CD 10_1101-2021_01_08_425864 349 8 significance significance NN 10_1101-2021_01_08_425864 349 9 as as IN 10_1101-2021_01_08_425864 349 10 follows follow VBZ 10_1101-2021_01_08_425864 349 11 : : : 10_1101-2021_01_08_425864 349 12 * * NFP 10_1101-2021_01_08_425864 349 13 * * NFP 10_1101-2021_01_08_425864 349 14 * * NFP 10_1101-2021_01_08_425864 349 15 p p NN 10_1101-2021_01_08_425864 349 16 value value NN 10_1101-2021_01_08_425864 349 17 < < XX 10_1101-2021_01_08_425864 349 18 0.001 0.001 CD 10_1101-2021_01_08_425864 349 19 ; ; : 10_1101-2021_01_08_425864 349 20 * * NFP 10_1101-2021_01_08_425864 349 21 * * NFP 10_1101-2021_01_08_425864 349 22 p p NN 10_1101-2021_01_08_425864 349 23 value value NN 10_1101-2021_01_08_425864 349 24 < < XX 10_1101-2021_01_08_425864 349 25 0.01 0.01 XX 10_1101-2021_01_08_425864 349 26 ; ; : 10_1101-2021_01_08_425864 349 27 and and CC 10_1101-2021_01_08_425864 349 28 * * NFP 10_1101-2021_01_08_425864 349 29 p p NN 10_1101-2021_01_08_425864 349 30 value value NN 10_1101-2021_01_08_425864 349 31 < < XX 10_1101-2021_01_08_425864 349 32 0.05 0.05 CD 10_1101-2021_01_08_425864 349 33 . . . 10_1101-2021_01_08_425864 350 1 For for IN 10_1101-2021_01_08_425864 350 2 p p NN 10_1101-2021_01_08_425864 350 3 values value NNS 10_1101-2021_01_08_425864 350 4 > > XX 10_1101-2021_01_08_425864 350 5 543 543 CD 10_1101-2021_01_08_425864 350 6 0.05 0.05 CD 10_1101-2021_01_08_425864 350 7 , , , 10_1101-2021_01_08_425864 350 8 we -PRON- PRP 10_1101-2021_01_08_425864 350 9 consider consider VBP 10_1101-2021_01_08_425864 350 10 the the DT 10_1101-2021_01_08_425864 350 11 differences difference NNS 10_1101-2021_01_08_425864 350 12 not not RB 10_1101-2021_01_08_425864 350 13 significant significant JJ 10_1101-2021_01_08_425864 350 14 ( ( -LRB- 10_1101-2021_01_08_425864 350 15 ns ns NNP 10_1101-2021_01_08_425864 350 16 ) ) -RRB- 10_1101-2021_01_08_425864 350 17 . . . 10_1101-2021_01_08_425864 351 1 544 544 CD 10_1101-2021_01_08_425864 351 2 545 545 CD 10_1101-2021_01_08_425864 351 3 Endogenous endogenous JJ 10_1101-2021_01_08_425864 351 4 fatty fatty NN 10_1101-2021_01_08_425864 351 5 acids acid NNS 10_1101-2021_01_08_425864 351 6 contribute contribute VBP 10_1101-2021_01_08_425864 351 7 to to IN 10_1101-2021_01_08_425864 351 8 long long JJ 10_1101-2021_01_08_425864 351 9 - - HYPH 10_1101-2021_01_08_425864 351 10 term term NN 10_1101-2021_01_08_425864 351 11 starvation starvation NN 10_1101-2021_01_08_425864 351 12 resistance resistance NN 10_1101-2021_01_08_425864 351 13 in in IN 10_1101-2021_01_08_425864 351 14 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 351 15 forms form NNS 10_1101-2021_01_08_425864 351 16 .CC .CC : 10_1101-2021_01_08_425864 351 17 - - : 10_1101-2021_01_08_425864 351 18 BY by IN 10_1101-2021_01_08_425864 351 19 4.0 4.0 CD 10_1101-2021_01_08_425864 351 20 International international JJ 10_1101-2021_01_08_425864 351 21 licensemade licensemade NN 10_1101-2021_01_08_425864 351 22 available available JJ 10_1101-2021_01_08_425864 351 23 under under IN 10_1101-2021_01_08_425864 351 24 a a DT 10_1101-2021_01_08_425864 351 25 ( ( -LRB- 10_1101-2021_01_08_425864 351 26 which which WDT 10_1101-2021_01_08_425864 351 27 was be VBD 10_1101-2021_01_08_425864 351 28 not not RB 10_1101-2021_01_08_425864 351 29 certified certify VBN 10_1101-2021_01_08_425864 351 30 by by IN 10_1101-2021_01_08_425864 351 31 peer peer NN 10_1101-2021_01_08_425864 351 32 review review NN 10_1101-2021_01_08_425864 351 33 ) ) -RRB- 10_1101-2021_01_08_425864 351 34 is be VBZ 10_1101-2021_01_08_425864 351 35 the the DT 10_1101-2021_01_08_425864 351 36 author author NN 10_1101-2021_01_08_425864 351 37 / / SYM 10_1101-2021_01_08_425864 351 38 funder funder NN 10_1101-2021_01_08_425864 351 39 , , , 10_1101-2021_01_08_425864 351 40 who who WP 10_1101-2021_01_08_425864 351 41 has have VBZ 10_1101-2021_01_08_425864 351 42 granted grant VBN 10_1101-2021_01_08_425864 351 43 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 351 44 a a DT 10_1101-2021_01_08_425864 351 45 license license NN 10_1101-2021_01_08_425864 351 46 to to TO 10_1101-2021_01_08_425864 351 47 display display VB 10_1101-2021_01_08_425864 351 48 the the DT 10_1101-2021_01_08_425864 351 49 preprint preprint NN 10_1101-2021_01_08_425864 351 50 in in IN 10_1101-2021_01_08_425864 351 51 perpetuity perpetuity NN 10_1101-2021_01_08_425864 351 52 . . . 10_1101-2021_01_08_425864 352 1 It -PRON- PRP 10_1101-2021_01_08_425864 352 2 is be VBZ 10_1101-2021_01_08_425864 352 3 The the DT 10_1101-2021_01_08_425864 352 4 copyright copyright NN 10_1101-2021_01_08_425864 352 5 holder holder NN 10_1101-2021_01_08_425864 352 6 for for IN 10_1101-2021_01_08_425864 352 7 this this DT 10_1101-2021_01_08_425864 352 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 352 9 version version NN 10_1101-2021_01_08_425864 352 10 posted post VBD 10_1101-2021_01_08_425864 352 11 January January NNP 10_1101-2021_01_08_425864 352 12 8 8 CD 10_1101-2021_01_08_425864 352 13 , , , 10_1101-2021_01_08_425864 352 14 2021 2021 CD 10_1101-2021_01_08_425864 352 15 . . . 10_1101-2021_01_08_425864 352 16 ; ; : 10_1101-2021_01_08_425864 352 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 352 18 : : : 10_1101-2021_01_08_425864 352 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 352 20 preprint preprint NN 10_1101-2021_01_08_425864 352 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 352 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 352 23 546 546 CD 10_1101-2021_01_08_425864 352 24 As as RB 10_1101-2021_01_08_425864 352 25 previously previously RB 10_1101-2021_01_08_425864 352 26 demonstrated demonstrate VBN 10_1101-2021_01_08_425864 352 27 , , , 10_1101-2021_01_08_425864 352 28 ETO ETO NNP 10_1101-2021_01_08_425864 352 29 interferes interfere VBZ 10_1101-2021_01_08_425864 352 30 with with IN 10_1101-2021_01_08_425864 352 31 the the DT 10_1101-2021_01_08_425864 352 32 consumption consumption NN 10_1101-2021_01_08_425864 352 33 of of IN 10_1101-2021_01_08_425864 352 34 endogenous endogenous JJ 10_1101-2021_01_08_425864 352 35 fatty fatty NN 10_1101-2021_01_08_425864 352 36 acids acid NNS 10_1101-2021_01_08_425864 352 37 , , , 10_1101-2021_01_08_425864 352 38 547 547 CD 10_1101-2021_01_08_425864 352 39 and and CC 10_1101-2021_01_08_425864 352 40 this this DT 10_1101-2021_01_08_425864 352 41 impairment impairment NN 10_1101-2021_01_08_425864 352 42 causes cause VBZ 10_1101-2021_01_08_425864 352 43 ATP atp NN 10_1101-2021_01_08_425864 352 44 depletion depletion NN 10_1101-2021_01_08_425864 352 45 and and CC 10_1101-2021_01_08_425864 352 46 cell cell NN 10_1101-2021_01_08_425864 352 47 cycle cycle NN 10_1101-2021_01_08_425864 352 48 arrest arrest NN 10_1101-2021_01_08_425864 352 49 . . . 10_1101-2021_01_08_425864 353 1 One one CD 10_1101-2021_01_08_425864 353 2 intriguing intriguing JJ 10_1101-2021_01_08_425864 353 3 characteristic characteristic NN 10_1101-2021_01_08_425864 353 4 of of IN 10_1101-2021_01_08_425864 353 5 the the DT 10_1101-2021_01_08_425864 353 6 548 548 CD 10_1101-2021_01_08_425864 353 7 insect insect NN 10_1101-2021_01_08_425864 353 8 stages stage NNS 10_1101-2021_01_08_425864 353 9 of of IN 10_1101-2021_01_08_425864 353 10 T. T. NNP 10_1101-2021_01_08_425864 353 11 cruzi cruzi NNS 10_1101-2021_01_08_425864 353 12 is be VBZ 10_1101-2021_01_08_425864 353 13 their -PRON- PRP$ 10_1101-2021_01_08_425864 353 14 resistance resistance NN 10_1101-2021_01_08_425864 353 15 to to IN 10_1101-2021_01_08_425864 353 16 starvation starvation NN 10_1101-2021_01_08_425864 353 17 . . . 10_1101-2021_01_08_425864 354 1 To to TO 10_1101-2021_01_08_425864 354 2 observe observe VB 10_1101-2021_01_08_425864 354 3 the the DT 10_1101-2021_01_08_425864 354 4 importance importance NN 10_1101-2021_01_08_425864 354 5 of of IN 10_1101-2021_01_08_425864 354 6 internal internal JJ 10_1101-2021_01_08_425864 354 7 fatty fatty NN 10_1101-2021_01_08_425864 354 8 549 549 CD 10_1101-2021_01_08_425864 354 9 acids acid NNS 10_1101-2021_01_08_425864 354 10 in in IN 10_1101-2021_01_08_425864 354 11 this this DT 10_1101-2021_01_08_425864 354 12 process process NN 10_1101-2021_01_08_425864 354 13 , , , 10_1101-2021_01_08_425864 354 14 we -PRON- PRP 10_1101-2021_01_08_425864 354 15 incubated incubate VBD 10_1101-2021_01_08_425864 354 16 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 354 17 in in IN 10_1101-2021_01_08_425864 354 18 PBS PBS NNP 10_1101-2021_01_08_425864 354 19 in in IN 10_1101-2021_01_08_425864 354 20 the the DT 10_1101-2021_01_08_425864 354 21 presence presence NN 10_1101-2021_01_08_425864 354 22 ( ( -LRB- 10_1101-2021_01_08_425864 354 23 or or CC 10_1101-2021_01_08_425864 354 24 absence absence VB 10_1101-2021_01_08_425864 354 25 ) ) -RRB- 10_1101-2021_01_08_425864 354 26 of of IN 10_1101-2021_01_08_425864 354 27 500 500 CD 10_1101-2021_01_08_425864 354 28 μM μM NNP 10_1101-2021_01_08_425864 354 29 ETO ETO NNP 10_1101-2021_01_08_425864 354 30 . . . 10_1101-2021_01_08_425864 355 1 550 550 CD 10_1101-2021_01_08_425864 355 2 The the DT 10_1101-2021_01_08_425864 355 3 mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 355 4 activity activity NN 10_1101-2021_01_08_425864 355 5 of of IN 10_1101-2021_01_08_425864 355 6 these these DT 10_1101-2021_01_08_425864 355 7 cells cell NNS 10_1101-2021_01_08_425864 355 8 was be VBD 10_1101-2021_01_08_425864 355 9 followed follow VBN 10_1101-2021_01_08_425864 355 10 for for IN 10_1101-2021_01_08_425864 355 11 24 24 CD 10_1101-2021_01_08_425864 355 12 h h NN 10_1101-2021_01_08_425864 355 13 with with IN 10_1101-2021_01_08_425864 355 14 Alamar Alamar NNP 10_1101-2021_01_08_425864 355 15 blue blue JJ 10_1101-2021_01_08_425864 355 16 ® ® NNPS 10_1101-2021_01_08_425864 355 17 . . . 10_1101-2021_01_08_425864 356 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 356 2 results result NNS 10_1101-2021_01_08_425864 356 3 551 551 CD 10_1101-2021_01_08_425864 356 4 showed show VBD 10_1101-2021_01_08_425864 356 5 that that IN 10_1101-2021_01_08_425864 356 6 the the DT 10_1101-2021_01_08_425864 356 7 mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 356 8 activity activity NN 10_1101-2021_01_08_425864 356 9 of of IN 10_1101-2021_01_08_425864 356 10 the the DT 10_1101-2021_01_08_425864 356 11 parasites parasite NNS 10_1101-2021_01_08_425864 356 12 in in IN 10_1101-2021_01_08_425864 356 13 the the DT 10_1101-2021_01_08_425864 356 14 presence presence NN 10_1101-2021_01_08_425864 356 15 of of IN 10_1101-2021_01_08_425864 356 16 ETO ETO NNP 10_1101-2021_01_08_425864 356 17 was be VBD 10_1101-2021_01_08_425864 356 18 reduced reduce VBN 10_1101-2021_01_08_425864 356 19 by by IN 10_1101-2021_01_08_425864 356 20 31 31 CD 10_1101-2021_01_08_425864 356 21 % % NN 10_1101-2021_01_08_425864 356 22 552 552 CD 10_1101-2021_01_08_425864 356 23 after after IN 10_1101-2021_01_08_425864 356 24 48 48 CD 10_1101-2021_01_08_425864 356 25 h h NN 10_1101-2021_01_08_425864 356 26 of of IN 10_1101-2021_01_08_425864 356 27 starvation starvation NN 10_1101-2021_01_08_425864 356 28 , , , 10_1101-2021_01_08_425864 356 29 and and CC 10_1101-2021_01_08_425864 356 30 65 65 CD 10_1101-2021_01_08_425864 356 31 % % NN 10_1101-2021_01_08_425864 356 32 after after IN 10_1101-2021_01_08_425864 356 33 72 72 CD 10_1101-2021_01_08_425864 356 34 h h NN 10_1101-2021_01_08_425864 356 35 of of IN 10_1101-2021_01_08_425864 356 36 starvation starvation NN 10_1101-2021_01_08_425864 356 37 ( ( -LRB- 10_1101-2021_01_08_425864 356 38 Fig fig NN 10_1101-2021_01_08_425864 356 39 . . . 10_1101-2021_01_08_425864 357 1 11 11 CD 10_1101-2021_01_08_425864 357 2 ) ) -RRB- 10_1101-2021_01_08_425864 357 3 compared compare VBN 10_1101-2021_01_08_425864 357 4 to to IN 10_1101-2021_01_08_425864 357 5 the the DT 10_1101-2021_01_08_425864 357 6 controls control NNS 10_1101-2021_01_08_425864 357 7 ( ( -LRB- 10_1101-2021_01_08_425864 357 8 untreated untreated JJ 10_1101-2021_01_08_425864 357 9 553 553 CD 10_1101-2021_01_08_425864 357 10 parasites parasite NNS 10_1101-2021_01_08_425864 357 11 ) ) -RRB- 10_1101-2021_01_08_425864 357 12 . . . 10_1101-2021_01_08_425864 358 1 These these DT 10_1101-2021_01_08_425864 358 2 data datum NNS 10_1101-2021_01_08_425864 358 3 confirmed confirm VBD 10_1101-2021_01_08_425864 358 4 our -PRON- PRP$ 10_1101-2021_01_08_425864 358 5 hypothesis hypothesis NN 10_1101-2021_01_08_425864 358 6 that that IN 10_1101-2021_01_08_425864 358 7 the the DT 10_1101-2021_01_08_425864 358 8 breakdown breakdown NN 10_1101-2021_01_08_425864 358 9 of of IN 10_1101-2021_01_08_425864 358 10 accumulated accumulated JJ 10_1101-2021_01_08_425864 358 11 fatty fatty NN 10_1101-2021_01_08_425864 358 12 acids acid NNS 10_1101-2021_01_08_425864 358 13 554 554 CD 10_1101-2021_01_08_425864 358 14 partially partially RB 10_1101-2021_01_08_425864 358 15 contributes contribute VBZ 10_1101-2021_01_08_425864 358 16 to to IN 10_1101-2021_01_08_425864 358 17 the the DT 10_1101-2021_01_08_425864 358 18 resistance resistance NN 10_1101-2021_01_08_425864 358 19 of of IN 10_1101-2021_01_08_425864 358 20 the the DT 10_1101-2021_01_08_425864 358 21 parasite parasite NN 10_1101-2021_01_08_425864 358 22 under under IN 10_1101-2021_01_08_425864 358 23 severe severe JJ 10_1101-2021_01_08_425864 358 24 starvation starvation NN 10_1101-2021_01_08_425864 358 25 . . . 10_1101-2021_01_08_425864 359 1 555 555 CD 10_1101-2021_01_08_425864 359 2 556 556 CD 10_1101-2021_01_08_425864 359 3 Figure figure NN 10_1101-2021_01_08_425864 359 4 11 11 CD 10_1101-2021_01_08_425864 359 5 . . . 10_1101-2021_01_08_425864 360 1 Internal internal JJ 10_1101-2021_01_08_425864 360 2 fatty fatty NN 10_1101-2021_01_08_425864 360 3 acid acid NN 10_1101-2021_01_08_425864 360 4 consumption consumption NN 10_1101-2021_01_08_425864 360 5 contributes contribute VBZ 10_1101-2021_01_08_425864 360 6 to to TO 10_1101-2021_01_08_425864 360 7 parasite parasite VB 10_1101-2021_01_08_425864 360 8 viability viability NN 10_1101-2021_01_08_425864 360 9 under under IN 10_1101-2021_01_08_425864 360 10 severe severe JJ 10_1101-2021_01_08_425864 360 11 557 557 CD 10_1101-2021_01_08_425864 360 12 nutritional nutritional JJ 10_1101-2021_01_08_425864 360 13 starvation starvation NN 10_1101-2021_01_08_425864 360 14 . . . 10_1101-2021_01_08_425864 361 1 Viability viability NN 10_1101-2021_01_08_425864 361 2 of of IN 10_1101-2021_01_08_425864 361 3 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 361 4 forms form NNS 10_1101-2021_01_08_425864 361 5 after after IN 10_1101-2021_01_08_425864 361 6 incubation incubation NN 10_1101-2021_01_08_425864 361 7 in in IN 10_1101-2021_01_08_425864 361 8 PBS PBS NNP 10_1101-2021_01_08_425864 361 9 with with IN 10_1101-2021_01_08_425864 361 10 or or CC 10_1101-2021_01_08_425864 361 11 without without IN 10_1101-2021_01_08_425864 361 12 ETO ETO NNP 10_1101-2021_01_08_425864 361 13 . . . 10_1101-2021_01_08_425864 362 1 558 558 CD 10_1101-2021_01_08_425864 362 2 The the DT 10_1101-2021_01_08_425864 362 3 viability viability NN 10_1101-2021_01_08_425864 362 4 was be VBD 10_1101-2021_01_08_425864 362 5 assessed assess VBN 10_1101-2021_01_08_425864 362 6 every every DT 10_1101-2021_01_08_425864 362 7 24 24 CD 10_1101-2021_01_08_425864 362 8 h h NN 10_1101-2021_01_08_425864 362 9 using use VBG 10_1101-2021_01_08_425864 362 10 Alamar Alamar NNP 10_1101-2021_01_08_425864 362 11 Blue Blue NNP 10_1101-2021_01_08_425864 362 12 ® ® NNP 10_1101-2021_01_08_425864 362 13 . . . 10_1101-2021_01_08_425864 363 1 Statistical statistical JJ 10_1101-2021_01_08_425864 363 2 analysis analysis NN 10_1101-2021_01_08_425864 363 3 was be VBD 10_1101-2021_01_08_425864 363 4 performed perform VBN 10_1101-2021_01_08_425864 363 5 with with IN 10_1101-2021_01_08_425864 363 6 559 559 CD 10_1101-2021_01_08_425864 363 7 one one CD 10_1101-2021_01_08_425864 363 8 - - HYPH 10_1101-2021_01_08_425864 363 9 way way NN 10_1101-2021_01_08_425864 363 10 ANOVA ANOVA NNP 10_1101-2021_01_08_425864 363 11 followed follow VBN 10_1101-2021_01_08_425864 363 12 by by IN 10_1101-2021_01_08_425864 363 13 Tukey Tukey NNP 10_1101-2021_01_08_425864 363 14 's 's POS 10_1101-2021_01_08_425864 363 15 post post JJ 10_1101-2021_01_08_425864 363 16 - - JJ 10_1101-2021_01_08_425864 363 17 test test JJ 10_1101-2021_01_08_425864 363 18 p p NN 10_1101-2021_01_08_425864 363 19 < < XX 10_1101-2021_01_08_425864 363 20 0.05 0.05 XX 10_1101-2021_01_08_425864 363 21 using use VBG 10_1101-2021_01_08_425864 363 22 GraphPad GraphPad NNP 10_1101-2021_01_08_425864 363 23 Prism Prism NNP 10_1101-2021_01_08_425864 363 24 8.0.2 8.0.2 CD 10_1101-2021_01_08_425864 363 25 software software NN 10_1101-2021_01_08_425864 363 26 . . . 10_1101-2021_01_08_425864 364 1 We -PRON- PRP 10_1101-2021_01_08_425864 364 2 560 560 CD 10_1101-2021_01_08_425864 364 3 represent represent VBP 10_1101-2021_01_08_425864 364 4 the the DT 10_1101-2021_01_08_425864 364 5 levels level NNS 10_1101-2021_01_08_425864 364 6 of of IN 10_1101-2021_01_08_425864 364 7 statistical statistical JJ 10_1101-2021_01_08_425864 364 8 significance significance NN 10_1101-2021_01_08_425864 364 9 as as IN 10_1101-2021_01_08_425864 364 10 follow follow NN 10_1101-2021_01_08_425864 364 11 : : : 10_1101-2021_01_08_425864 364 12 * * NFP 10_1101-2021_01_08_425864 364 13 * * NFP 10_1101-2021_01_08_425864 364 14 * * NFP 10_1101-2021_01_08_425864 364 15 p p NN 10_1101-2021_01_08_425864 364 16 value value NN 10_1101-2021_01_08_425864 364 17 < < XX 10_1101-2021_01_08_425864 364 18 0.001 0.001 CD 10_1101-2021_01_08_425864 364 19 , , , 10_1101-2021_01_08_425864 364 20 and and CC 10_1101-2021_01_08_425864 364 21 for for IN 10_1101-2021_01_08_425864 364 22 p p NN 10_1101-2021_01_08_425864 364 23 values value NNS 10_1101-2021_01_08_425864 364 24 > > XX 10_1101-2021_01_08_425864 364 25 0.05 0.05 CD 10_1101-2021_01_08_425864 364 26 , , , 10_1101-2021_01_08_425864 364 27 561 561 CD 10_1101-2021_01_08_425864 364 28 we -PRON- PRP 10_1101-2021_01_08_425864 364 29 consider consider VBP 10_1101-2021_01_08_425864 364 30 the the DT 10_1101-2021_01_08_425864 364 31 differences difference NNS 10_1101-2021_01_08_425864 364 32 not not RB 10_1101-2021_01_08_425864 364 33 significant significant JJ 10_1101-2021_01_08_425864 364 34 ( ( -LRB- 10_1101-2021_01_08_425864 364 35 ns ns NNP 10_1101-2021_01_08_425864 364 36 ) ) -RRB- 10_1101-2021_01_08_425864 364 37 . . . 10_1101-2021_01_08_425864 365 1 562 562 CD 10_1101-2021_01_08_425864 365 2 563 563 CD 10_1101-2021_01_08_425864 365 3 Inhibition Inhibition NNP 10_1101-2021_01_08_425864 365 4 of of IN 10_1101-2021_01_08_425864 365 5 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 365 6 impairs impairs NNP 10_1101-2021_01_08_425864 365 7 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 365 8 564 564 CD 10_1101-2021_01_08_425864 365 9 Considering consider VBG 10_1101-2021_01_08_425864 365 10 that that IN 10_1101-2021_01_08_425864 365 11 the the DT 10_1101-2021_01_08_425864 365 12 FAO FAO NNP 10_1101-2021_01_08_425864 365 13 increases increase VBZ 10_1101-2021_01_08_425864 365 14 in in IN 10_1101-2021_01_08_425864 365 15 the the DT 10_1101-2021_01_08_425864 365 16 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 365 17 during during IN 10_1101-2021_01_08_425864 365 18 the the DT 10_1101-2021_01_08_425864 365 19 stationary stationary JJ 10_1101-2021_01_08_425864 365 20 phase phase NN 10_1101-2021_01_08_425864 365 21 , , , 10_1101-2021_01_08_425864 365 22 and and CC 10_1101-2021_01_08_425864 365 23 that that IN 10_1101-2021_01_08_425864 365 24 565 565 CD 10_1101-2021_01_08_425864 365 25 differentiation differentiation NN 10_1101-2021_01_08_425864 365 26 into into IN 10_1101-2021_01_08_425864 365 27 infective infective JJ 10_1101-2021_01_08_425864 365 28 metacyclic metacyclic JJ 10_1101-2021_01_08_425864 365 29 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 365 30 ( ( -LRB- 10_1101-2021_01_08_425864 365 31 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 365 32 ) ) -RRB- 10_1101-2021_01_08_425864 365 33 is be VBZ 10_1101-2021_01_08_425864 365 34 triggered trigger VBN 10_1101-2021_01_08_425864 365 35 in in IN 10_1101-2021_01_08_425864 365 36 the the DT 10_1101-2021_01_08_425864 365 37 566 566 CD 10_1101-2021_01_08_425864 365 38 stationary stationary JJ 10_1101-2021_01_08_425864 365 39 phase phase NN 10_1101-2021_01_08_425864 365 40 of of IN 10_1101-2021_01_08_425864 365 41 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 365 42 parasites parasite NNS 10_1101-2021_01_08_425864 365 43 , , , 10_1101-2021_01_08_425864 365 44 one one PRP 10_1101-2021_01_08_425864 365 45 might may MD 10_1101-2021_01_08_425864 365 46 expect expect VB 10_1101-2021_01_08_425864 365 47 a a DT 10_1101-2021_01_08_425864 365 48 possible possible JJ 10_1101-2021_01_08_425864 365 49 relationship relationship NN 10_1101-2021_01_08_425864 365 50 between between IN 10_1101-2021_01_08_425864 365 51 the the DT 10_1101-2021_01_08_425864 365 52 567 567 CD 10_1101-2021_01_08_425864 365 53 consumption consumption NN 10_1101-2021_01_08_425864 365 54 of of IN 10_1101-2021_01_08_425864 365 55 fatty fatty NN 10_1101-2021_01_08_425864 365 56 acids acid NNS 10_1101-2021_01_08_425864 365 57 and and CC 10_1101-2021_01_08_425864 365 58 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 365 59 . . . 10_1101-2021_01_08_425864 366 1 To to TO 10_1101-2021_01_08_425864 366 2 approach approach VB 10_1101-2021_01_08_425864 366 3 this this DT 10_1101-2021_01_08_425864 366 4 possibility possibility NN 10_1101-2021_01_08_425864 366 5 , , , 10_1101-2021_01_08_425864 366 6 we -PRON- PRP 10_1101-2021_01_08_425864 366 7 initially initially RB 10_1101-2021_01_08_425864 366 8 compared compare VBD 10_1101-2021_01_08_425864 366 9 568 568 CD 10_1101-2021_01_08_425864 366 10 the the DT 10_1101-2021_01_08_425864 366 11 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 366 12 activity activity NN 10_1101-2021_01_08_425864 366 13 of of IN 10_1101-2021_01_08_425864 366 14 stationary stationary JJ 10_1101-2021_01_08_425864 366 15 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 366 16 forms form NNS 10_1101-2021_01_08_425864 366 17 before before IN 10_1101-2021_01_08_425864 366 18 and and CC 10_1101-2021_01_08_425864 366 19 after after IN 10_1101-2021_01_08_425864 366 20 a a DT 10_1101-2021_01_08_425864 366 21 24 24 CD 10_1101-2021_01_08_425864 366 22 h h NN 10_1101-2021_01_08_425864 366 23 incubation incubation NN 10_1101-2021_01_08_425864 366 24 in in IN 10_1101-2021_01_08_425864 366 25 the the DT 10_1101-2021_01_08_425864 366 26 569 569 CD 10_1101-2021_01_08_425864 366 27 differentiation differentiation NN 10_1101-2021_01_08_425864 366 28 medium medium NN 10_1101-2021_01_08_425864 366 29 TAU-3AAG TAU-3AAG NNP 10_1101-2021_01_08_425864 366 30 . . . 10_1101-2021_01_08_425864 367 1 As as IN 10_1101-2021_01_08_425864 367 2 observed observe VBN 10_1101-2021_01_08_425864 367 3 , , , 10_1101-2021_01_08_425864 367 4 there there EX 10_1101-2021_01_08_425864 367 5 is be VBZ 10_1101-2021_01_08_425864 367 6 an an DT 10_1101-2021_01_08_425864 367 7 increase increase NN 10_1101-2021_01_08_425864 367 8 in in IN 10_1101-2021_01_08_425864 367 9 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 367 10 activity activity NN 10_1101-2021_01_08_425864 367 11 after after IN 10_1101-2021_01_08_425864 367 12 570 570 CD 10_1101-2021_01_08_425864 367 13 submitting submit VBG 10_1101-2021_01_08_425864 367 14 the the DT 10_1101-2021_01_08_425864 367 15 parasites parasite NNS 10_1101-2021_01_08_425864 367 16 to to IN 10_1101-2021_01_08_425864 367 17 the the DT 10_1101-2021_01_08_425864 367 18 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 367 19 in in IN 10_1101-2021_01_08_425864 367 20 vitro vitro FW 10_1101-2021_01_08_425864 367 21 ( ( -LRB- 10_1101-2021_01_08_425864 367 22 Fig fig NN 10_1101-2021_01_08_425864 367 23 . . . 10_1101-2021_01_08_425864 368 1 12A 12a LS 10_1101-2021_01_08_425864 368 2 ) ) -RRB- 10_1101-2021_01_08_425864 368 3 . . . 10_1101-2021_01_08_425864 369 1 Parasites parasite NNS 10_1101-2021_01_08_425864 369 2 were be VBD 10_1101-2021_01_08_425864 369 3 then then RB 10_1101-2021_01_08_425864 369 4 submitted submit VBN 10_1101-2021_01_08_425864 369 5 to to IN 10_1101-2021_01_08_425864 369 6 571 571 CD 10_1101-2021_01_08_425864 369 7 differentiation differentiation NN 10_1101-2021_01_08_425864 369 8 with with IN 10_1101-2021_01_08_425864 369 9 TAU-3AAG TAU-3AAG NNP 10_1101-2021_01_08_425864 369 10 medium medium NNP 10_1101-2021_01_08_425864 369 11 in in IN 10_1101-2021_01_08_425864 369 12 the the DT 10_1101-2021_01_08_425864 369 13 presence presence NN 10_1101-2021_01_08_425864 369 14 of of IN 10_1101-2021_01_08_425864 369 15 the the DT 10_1101-2021_01_08_425864 369 16 probe probe NN 10_1101-2021_01_08_425864 369 17 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 369 18 . . . 10_1101-2021_01_08_425864 370 1 The the DT 10_1101-2021_01_08_425864 370 2 probe probe NN 10_1101-2021_01_08_425864 370 3 was be VBD 10_1101-2021_01_08_425864 370 4 572 572 CD 10_1101-2021_01_08_425864 370 5 incorporated incorporate VBN 10_1101-2021_01_08_425864 370 6 into into IN 10_1101-2021_01_08_425864 370 7 lipid lipid NN 10_1101-2021_01_08_425864 370 8 droplets droplet NNS 10_1101-2021_01_08_425864 370 9 , , , 10_1101-2021_01_08_425864 370 10 confirming confirm VBG 10_1101-2021_01_08_425864 370 11 that that IN 10_1101-2021_01_08_425864 370 12 fatty fatty NN 10_1101-2021_01_08_425864 370 13 acids acid NNS 10_1101-2021_01_08_425864 370 14 metabolism metabolism NN 10_1101-2021_01_08_425864 370 15 was be VBD 10_1101-2021_01_08_425864 370 16 active active JJ 10_1101-2021_01_08_425864 370 17 during during IN 10_1101-2021_01_08_425864 370 18 the the DT 10_1101-2021_01_08_425864 370 19 573 573 CD 10_1101-2021_01_08_425864 370 20 beginning beginning NN 10_1101-2021_01_08_425864 370 21 of of IN 10_1101-2021_01_08_425864 370 22 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 370 23 ( ( -LRB- 10_1101-2021_01_08_425864 370 24 Fig Fig NNP 10_1101-2021_01_08_425864 370 25 12B 12B NNP 10_1101-2021_01_08_425864 370 26 ) ) -RRB- 10_1101-2021_01_08_425864 370 27 . . . 10_1101-2021_01_08_425864 371 1 To to TO 10_1101-2021_01_08_425864 371 2 address address VB 10_1101-2021_01_08_425864 371 3 the the DT 10_1101-2021_01_08_425864 371 4 importance importance NN 10_1101-2021_01_08_425864 371 5 of of IN 10_1101-2021_01_08_425864 371 6 FAO FAO NNP 10_1101-2021_01_08_425864 371 7 during during IN 10_1101-2021_01_08_425864 371 8 differentiation differentiation NN 10_1101-2021_01_08_425864 371 9 , , , 10_1101-2021_01_08_425864 371 10 574 574 CD 10_1101-2021_01_08_425864 371 11 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 371 12 was be VBD 10_1101-2021_01_08_425864 371 13 induced induce VBN 10_1101-2021_01_08_425864 371 14 in in IN 10_1101-2021_01_08_425864 371 15 vitro vitro FW 10_1101-2021_01_08_425864 371 16 on on IN 10_1101-2021_01_08_425864 371 17 ETO ETO NNP 10_1101-2021_01_08_425864 371 18 - - HYPH 10_1101-2021_01_08_425864 371 19 treated treat VBN 10_1101-2021_01_08_425864 371 20 or or CC 10_1101-2021_01_08_425864 371 21 untreated untreated JJ 10_1101-2021_01_08_425864 371 22 ( ( -LRB- 10_1101-2021_01_08_425864 371 23 control control NN 10_1101-2021_01_08_425864 371 24 ) ) -RRB- 10_1101-2021_01_08_425864 371 25 parasites parasite NNS 10_1101-2021_01_08_425864 371 26 . . . 10_1101-2021_01_08_425864 372 1 ETO ETO NNP 10_1101-2021_01_08_425864 372 2 575 575 CD 10_1101-2021_01_08_425864 372 3 treatment treatment NN 10_1101-2021_01_08_425864 372 4 interfered interfere VBN 10_1101-2021_01_08_425864 372 5 with with IN 10_1101-2021_01_08_425864 372 6 differentiation differentiation NN 10_1101-2021_01_08_425864 372 7 , , , 10_1101-2021_01_08_425864 372 8 diminishing diminish VBG 10_1101-2021_01_08_425864 372 9 the the DT 10_1101-2021_01_08_425864 372 10 number number NN 10_1101-2021_01_08_425864 372 11 of of IN 10_1101-2021_01_08_425864 372 12 metacyclic metacyclic JJ 10_1101-2021_01_08_425864 372 13 forms form NNS 10_1101-2021_01_08_425864 372 14 present present JJ 10_1101-2021_01_08_425864 372 15 in in IN 10_1101-2021_01_08_425864 372 16 the the DT 10_1101-2021_01_08_425864 372 17 576 576 CD 10_1101-2021_01_08_425864 372 18 culture culture NN 10_1101-2021_01_08_425864 372 19 ( ( -LRB- 10_1101-2021_01_08_425864 372 20 Fig Fig NNP 10_1101-2021_01_08_425864 372 21 12C 12C NNP 10_1101-2021_01_08_425864 372 22 ) ) -RRB- 10_1101-2021_01_08_425864 372 23 . . . 10_1101-2021_01_08_425864 373 1 In in IN 10_1101-2021_01_08_425864 373 2 addition addition NN 10_1101-2021_01_08_425864 373 3 , , , 10_1101-2021_01_08_425864 373 4 this this DT 10_1101-2021_01_08_425864 373 5 inhibition inhibition NN 10_1101-2021_01_08_425864 373 6 was be VBD 10_1101-2021_01_08_425864 373 7 dose dose RB 10_1101-2021_01_08_425864 373 8 - - HYPH 10_1101-2021_01_08_425864 373 9 dependent dependent JJ 10_1101-2021_01_08_425864 373 10 , , , 10_1101-2021_01_08_425864 373 11 with with IN 10_1101-2021_01_08_425864 373 12 an an DT 10_1101-2021_01_08_425864 373 13 IC50 IC50 NNP 10_1101-2021_01_08_425864 373 14 = = SYM 10_1101-2021_01_08_425864 373 15 + + CC 10_1101-2021_01_08_425864 373 16 32.96 32.96 CD 10_1101-2021_01_08_425864 373 17 µM µM NNP 10_1101-2021_01_08_425864 373 18 ( ( -LRB- 10_1101-2021_01_08_425864 373 19 Fig fig NN 10_1101-2021_01_08_425864 373 20 577 577 CD 10_1101-2021_01_08_425864 373 21 12D 12d CD 10_1101-2021_01_08_425864 373 22 ) ) -RRB- 10_1101-2021_01_08_425864 373 23 . . . 10_1101-2021_01_08_425864 374 1 Importantly importantly RB 10_1101-2021_01_08_425864 374 2 , , , 10_1101-2021_01_08_425864 374 3 we -PRON- PRP 10_1101-2021_01_08_425864 374 4 ruled rule VBD 10_1101-2021_01_08_425864 374 5 out out RP 10_1101-2021_01_08_425864 374 6 that that IN 10_1101-2021_01_08_425864 374 7 the the DT 10_1101-2021_01_08_425864 374 8 variation variation NN 10_1101-2021_01_08_425864 374 9 found find VBN 10_1101-2021_01_08_425864 374 10 in in IN 10_1101-2021_01_08_425864 374 11 the the DT 10_1101-2021_01_08_425864 374 12 differentiation differentiation NN 10_1101-2021_01_08_425864 374 13 rates rate NNS 10_1101-2021_01_08_425864 374 14 was be VBD 10_1101-2021_01_08_425864 374 15 due due JJ 10_1101-2021_01_08_425864 374 16 to to IN 10_1101-2021_01_08_425864 374 17 a a DT 10_1101-2021_01_08_425864 374 18 578 578 CD 10_1101-2021_01_08_425864 374 19 selective selective JJ 10_1101-2021_01_08_425864 374 20 death death NN 10_1101-2021_01_08_425864 374 21 of of IN 10_1101-2021_01_08_425864 374 22 treated treat VBN 10_1101-2021_01_08_425864 374 23 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 374 24 , , , 10_1101-2021_01_08_425864 374 25 since since IN 10_1101-2021_01_08_425864 374 26 their -PRON- PRP$ 10_1101-2021_01_08_425864 374 27 survival survival NN 10_1101-2021_01_08_425864 374 28 during during IN 10_1101-2021_01_08_425864 374 29 this this DT 10_1101-2021_01_08_425864 374 30 experiment experiment NN 10_1101-2021_01_08_425864 374 31 in in IN 10_1101-2021_01_08_425864 374 32 the the DT 10_1101-2021_01_08_425864 374 33 presence presence NN 10_1101-2021_01_08_425864 374 34 or or CC 10_1101-2021_01_08_425864 374 35 579 579 CD 10_1101-2021_01_08_425864 374 36 absence absence NN 10_1101-2021_01_08_425864 374 37 of of IN 10_1101-2021_01_08_425864 374 38 ETO ETO NNP 10_1101-2021_01_08_425864 374 39 ( ( -LRB- 10_1101-2021_01_08_425864 374 40 from from IN 10_1101-2021_01_08_425864 374 41 5 5 CD 10_1101-2021_01_08_425864 374 42 to to IN 10_1101-2021_01_08_425864 374 43 500 500 CD 10_1101-2021_01_08_425864 374 44 µM µM NNP 10_1101-2021_01_08_425864 374 45 ) ) -RRB- 10_1101-2021_01_08_425864 374 46 was be VBD 10_1101-2021_01_08_425864 374 47 not not RB 10_1101-2021_01_08_425864 374 48 significantly significantly RB 10_1101-2021_01_08_425864 374 49 different different JJ 10_1101-2021_01_08_425864 374 50 ( ( -LRB- 10_1101-2021_01_08_425864 374 51 S5 s5 NN 10_1101-2021_01_08_425864 374 52 Fig Fig NNP 10_1101-2021_01_08_425864 374 53 ) ) -RRB- 10_1101-2021_01_08_425864 374 54 . . . 10_1101-2021_01_08_425864 375 1 Based base VBN 10_1101-2021_01_08_425864 375 2 on on IN 10_1101-2021_01_08_425864 375 3 these these DT 10_1101-2021_01_08_425864 375 4 data datum NNS 10_1101-2021_01_08_425864 375 5 , , , 10_1101-2021_01_08_425864 375 6 we -PRON- PRP 10_1101-2021_01_08_425864 375 7 580 580 CD 10_1101-2021_01_08_425864 375 8 could could MD 10_1101-2021_01_08_425864 375 9 conclude conclude VB 10_1101-2021_01_08_425864 375 10 that that IN 10_1101-2021_01_08_425864 375 11 fatty fatty NN 10_1101-2021_01_08_425864 375 12 acid acid NN 10_1101-2021_01_08_425864 375 13 oxidation oxidation NN 10_1101-2021_01_08_425864 375 14 , , , 10_1101-2021_01_08_425864 375 15 at at IN 10_1101-2021_01_08_425864 375 16 the the DT 10_1101-2021_01_08_425864 375 17 level level NN 10_1101-2021_01_08_425864 375 18 of of IN 10_1101-2021_01_08_425864 375 19 the the DT 10_1101-2021_01_08_425864 375 20 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 375 21 , , , 10_1101-2021_01_08_425864 375 22 was be VBD 10_1101-2021_01_08_425864 375 23 also also RB 10_1101-2021_01_08_425864 375 24 participating participate VBG 10_1101-2021_01_08_425864 375 25 in in IN 10_1101-2021_01_08_425864 375 26 the the DT 10_1101-2021_01_08_425864 375 27 581 581 CD 10_1101-2021_01_08_425864 375 28 regulation regulation NN 10_1101-2021_01_08_425864 375 29 of of IN 10_1101-2021_01_08_425864 375 30 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 375 31 . . . 10_1101-2021_01_08_425864 376 1 582 582 CD 10_1101-2021_01_08_425864 376 2 .CC .CC NFP 10_1101-2021_01_08_425864 376 3 - - : 10_1101-2021_01_08_425864 376 4 BY by IN 10_1101-2021_01_08_425864 376 5 4.0 4.0 CD 10_1101-2021_01_08_425864 376 6 International international JJ 10_1101-2021_01_08_425864 376 7 licensemade licensemade NN 10_1101-2021_01_08_425864 376 8 available available JJ 10_1101-2021_01_08_425864 376 9 under under IN 10_1101-2021_01_08_425864 376 10 a a DT 10_1101-2021_01_08_425864 376 11 ( ( -LRB- 10_1101-2021_01_08_425864 376 12 which which WDT 10_1101-2021_01_08_425864 376 13 was be VBD 10_1101-2021_01_08_425864 376 14 not not RB 10_1101-2021_01_08_425864 376 15 certified certify VBN 10_1101-2021_01_08_425864 376 16 by by IN 10_1101-2021_01_08_425864 376 17 peer peer NN 10_1101-2021_01_08_425864 376 18 review review NN 10_1101-2021_01_08_425864 376 19 ) ) -RRB- 10_1101-2021_01_08_425864 376 20 is be VBZ 10_1101-2021_01_08_425864 376 21 the the DT 10_1101-2021_01_08_425864 376 22 author author NN 10_1101-2021_01_08_425864 376 23 / / SYM 10_1101-2021_01_08_425864 376 24 funder funder NN 10_1101-2021_01_08_425864 376 25 , , , 10_1101-2021_01_08_425864 376 26 who who WP 10_1101-2021_01_08_425864 376 27 has have VBZ 10_1101-2021_01_08_425864 376 28 granted grant VBN 10_1101-2021_01_08_425864 376 29 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 376 30 a a DT 10_1101-2021_01_08_425864 376 31 license license NN 10_1101-2021_01_08_425864 376 32 to to TO 10_1101-2021_01_08_425864 376 33 display display VB 10_1101-2021_01_08_425864 376 34 the the DT 10_1101-2021_01_08_425864 376 35 preprint preprint NN 10_1101-2021_01_08_425864 376 36 in in IN 10_1101-2021_01_08_425864 376 37 perpetuity perpetuity NN 10_1101-2021_01_08_425864 376 38 . . . 10_1101-2021_01_08_425864 377 1 It -PRON- PRP 10_1101-2021_01_08_425864 377 2 is be VBZ 10_1101-2021_01_08_425864 377 3 The the DT 10_1101-2021_01_08_425864 377 4 copyright copyright NN 10_1101-2021_01_08_425864 377 5 holder holder NN 10_1101-2021_01_08_425864 377 6 for for IN 10_1101-2021_01_08_425864 377 7 this this DT 10_1101-2021_01_08_425864 377 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 377 9 version version NN 10_1101-2021_01_08_425864 377 10 posted post VBD 10_1101-2021_01_08_425864 377 11 January January NNP 10_1101-2021_01_08_425864 377 12 8 8 CD 10_1101-2021_01_08_425864 377 13 , , , 10_1101-2021_01_08_425864 377 14 2021 2021 CD 10_1101-2021_01_08_425864 377 15 . . . 10_1101-2021_01_08_425864 377 16 ; ; : 10_1101-2021_01_08_425864 377 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 377 18 : : : 10_1101-2021_01_08_425864 377 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 377 20 preprint preprint NN 10_1101-2021_01_08_425864 377 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 377 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 377 23 583 583 CD 10_1101-2021_01_08_425864 377 24 Figure Figure NNP 10_1101-2021_01_08_425864 377 25 12 12 CD 10_1101-2021_01_08_425864 377 26 . . . 10_1101-2021_01_08_425864 378 1 ETO ETO NNP 10_1101-2021_01_08_425864 378 2 inhibits inhibit VBZ 10_1101-2021_01_08_425864 378 3 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 378 4 . . . 10_1101-2021_01_08_425864 379 1 A a LS 10_1101-2021_01_08_425864 379 2 ) ) -RRB- 10_1101-2021_01_08_425864 379 3 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 379 4 activity activity NN 10_1101-2021_01_08_425864 379 5 of of IN 10_1101-2021_01_08_425864 379 6 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 379 7 forms form NNS 10_1101-2021_01_08_425864 379 8 in in IN 10_1101-2021_01_08_425864 379 9 stationary stationary JJ 10_1101-2021_01_08_425864 379 10 584 584 CD 10_1101-2021_01_08_425864 379 11 phase phase NN 10_1101-2021_01_08_425864 379 12 and and CC 10_1101-2021_01_08_425864 379 13 24h 24h NNS 10_1101-2021_01_08_425864 379 14 after after IN 10_1101-2021_01_08_425864 379 15 incubated incubate VBD 10_1101-2021_01_08_425864 379 16 in in IN 10_1101-2021_01_08_425864 379 17 TAU-3AAG TAU-3AAG NNP 10_1101-2021_01_08_425864 379 18 medium medium NNP 10_1101-2021_01_08_425864 379 19 ( ( -LRB- 10_1101-2021_01_08_425864 379 20 for for IN 10_1101-2021_01_08_425864 379 21 triggering trigger VBG 10_1101-2021_01_08_425864 379 22 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 379 23 ) ) -RRB- 10_1101-2021_01_08_425864 379 24 . . . 10_1101-2021_01_08_425864 380 1 B b NN 10_1101-2021_01_08_425864 380 2 ) ) -RRB- 10_1101-2021_01_08_425864 380 3 585 585 CD 10_1101-2021_01_08_425864 380 4 Fluorescence fluorescence NN 10_1101-2021_01_08_425864 380 5 microscopy microscopy NN 10_1101-2021_01_08_425864 380 6 of of IN 10_1101-2021_01_08_425864 380 7 cells cell NNS 10_1101-2021_01_08_425864 380 8 incubated incubate VBN 10_1101-2021_01_08_425864 380 9 in in IN 10_1101-2021_01_08_425864 380 10 TAU-3AAG TAU-3AAG NNP 10_1101-2021_01_08_425864 380 11 in in IN 10_1101-2021_01_08_425864 380 12 the the DT 10_1101-2021_01_08_425864 380 13 presence presence NN 10_1101-2021_01_08_425864 380 14 of of IN 10_1101-2021_01_08_425864 380 15 BODIPY BODIPY NNP 10_1101-2021_01_08_425864 380 16 ® ® NNP 10_1101-2021_01_08_425864 380 17 500 500 CD 10_1101-2021_01_08_425864 380 18 - - SYM 10_1101-2021_01_08_425864 380 19 510 510 CD 10_1101-2021_01_08_425864 380 20 586 586 CD 10_1101-2021_01_08_425864 380 21 C1-C12 C1-C12 NNP 10_1101-2021_01_08_425864 380 22 . . . 10_1101-2021_01_08_425864 381 1 C C NNP 10_1101-2021_01_08_425864 381 2 ) ) -RRB- 10_1101-2021_01_08_425864 381 3 Effects effect NNS 10_1101-2021_01_08_425864 381 4 of of IN 10_1101-2021_01_08_425864 381 5 different different JJ 10_1101-2021_01_08_425864 381 6 ETO eto NN 10_1101-2021_01_08_425864 381 7 concentrations concentration NNS 10_1101-2021_01_08_425864 381 8 on on IN 10_1101-2021_01_08_425864 381 9 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 381 10 . . . 10_1101-2021_01_08_425864 382 1 The the DT 10_1101-2021_01_08_425864 382 2 differentiation differentiation NN 10_1101-2021_01_08_425864 382 3 was be VBD 10_1101-2021_01_08_425864 382 4 587 587 CD 10_1101-2021_01_08_425864 382 5 evaluated evaluate VBN 10_1101-2021_01_08_425864 382 6 by by IN 10_1101-2021_01_08_425864 382 7 counting count VBG 10_1101-2021_01_08_425864 382 8 the the DT 10_1101-2021_01_08_425864 382 9 cells cell NNS 10_1101-2021_01_08_425864 382 10 in in IN 10_1101-2021_01_08_425864 382 11 a a DT 10_1101-2021_01_08_425864 382 12 Neubauer Neubauer NNP 10_1101-2021_01_08_425864 382 13 chamber chamber NN 10_1101-2021_01_08_425864 382 14 each each DT 10_1101-2021_01_08_425864 382 15 day day NN 10_1101-2021_01_08_425864 382 16 for for IN 10_1101-2021_01_08_425864 382 17 6 6 CD 10_1101-2021_01_08_425864 382 18 days day NNS 10_1101-2021_01_08_425864 382 19 . . . 10_1101-2021_01_08_425864 383 1 This this DT 10_1101-2021_01_08_425864 383 2 experiment experiment NN 10_1101-2021_01_08_425864 383 3 was be VBD 10_1101-2021_01_08_425864 383 4 588 588 CD 10_1101-2021_01_08_425864 383 5 performed perform VBN 10_1101-2021_01_08_425864 383 6 in in IN 10_1101-2021_01_08_425864 383 7 triplicate triplicate NN 10_1101-2021_01_08_425864 383 8 . . . 10_1101-2021_01_08_425864 384 1 D d LS 10_1101-2021_01_08_425864 384 2 ) ) -RRB- 10_1101-2021_01_08_425864 384 3 Percentage percentage NN 10_1101-2021_01_08_425864 384 4 of of IN 10_1101-2021_01_08_425864 384 5 differentiation differentiation NN 10_1101-2021_01_08_425864 384 6 at at IN 10_1101-2021_01_08_425864 384 7 the the DT 10_1101-2021_01_08_425864 384 8 5th 5th JJ 10_1101-2021_01_08_425864 384 9 day day NN 10_1101-2021_01_08_425864 384 10 of of IN 10_1101-2021_01_08_425864 384 11 differentiation differentiation NN 10_1101-2021_01_08_425864 384 12 . . . 10_1101-2021_01_08_425864 385 1 Inset inset NN 10_1101-2021_01_08_425864 385 2 : : : 10_1101-2021_01_08_425864 385 3 IC50 IC50 NNP 10_1101-2021_01_08_425864 385 4 of of IN 10_1101-2021_01_08_425864 385 5 589 589 CD 10_1101-2021_01_08_425864 385 6 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 385 7 inhibition inhibition NN 10_1101-2021_01_08_425864 385 8 by by IN 10_1101-2021_01_08_425864 385 9 ETO ETO NNP 10_1101-2021_01_08_425864 385 10 . . . 10_1101-2021_01_08_425864 386 1 The the DT 10_1101-2021_01_08_425864 386 2 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 386 3 activities activity NNS 10_1101-2021_01_08_425864 386 4 were be VBD 10_1101-2021_01_08_425864 386 5 measured measure VBN 10_1101-2021_01_08_425864 386 6 in in IN 10_1101-2021_01_08_425864 386 7 duplicate duplicate NN 10_1101-2021_01_08_425864 386 8 . . . 10_1101-2021_01_08_425864 387 1 All all PDT 10_1101-2021_01_08_425864 387 2 the the DT 10_1101-2021_01_08_425864 387 3 590 590 CD 10_1101-2021_01_08_425864 387 4 other other JJ 10_1101-2021_01_08_425864 387 5 experiments experiment NNS 10_1101-2021_01_08_425864 387 6 were be VBD 10_1101-2021_01_08_425864 387 7 performed perform VBN 10_1101-2021_01_08_425864 387 8 in in IN 10_1101-2021_01_08_425864 387 9 triplicates triplicate NNS 10_1101-2021_01_08_425864 387 10 . . . 10_1101-2021_01_08_425864 388 1 591 591 CD 10_1101-2021_01_08_425864 388 2 592 592 CD 10_1101-2021_01_08_425864 388 3 593 593 CD 10_1101-2021_01_08_425864 388 4 Discussion discussion NN 10_1101-2021_01_08_425864 388 5 594 594 CD 10_1101-2021_01_08_425864 388 6 During during IN 10_1101-2021_01_08_425864 388 7 the the DT 10_1101-2021_01_08_425864 388 8 journey journey NN 10_1101-2021_01_08_425864 388 9 of of IN 10_1101-2021_01_08_425864 388 10 T. T. NNP 10_1101-2021_01_08_425864 388 11 cruzi cruzi NNS 10_1101-2021_01_08_425864 388 12 inside inside IN 10_1101-2021_01_08_425864 388 13 the the DT 10_1101-2021_01_08_425864 388 14 insect insect JJ 10_1101-2021_01_08_425864 388 15 vector vector NN 10_1101-2021_01_08_425864 388 16 , , , 10_1101-2021_01_08_425864 388 17 the the DT 10_1101-2021_01_08_425864 388 18 glucose glucose NN 10_1101-2021_01_08_425864 388 19 levels level NNS 10_1101-2021_01_08_425864 388 20 decrease decrease VBP 10_1101-2021_01_08_425864 388 21 rapidly rapidly RB 10_1101-2021_01_08_425864 388 22 after after IN 10_1101-2021_01_08_425864 388 23 595 595 CD 10_1101-2021_01_08_425864 388 24 each each DT 10_1101-2021_01_08_425864 388 25 blood blood NN 10_1101-2021_01_08_425864 388 26 meal meal NN 10_1101-2021_01_08_425864 388 27 [ [ -LRB- 10_1101-2021_01_08_425864 388 28 34 34 CD 10_1101-2021_01_08_425864 388 29 ] ] -RRB- 10_1101-2021_01_08_425864 388 30 , , , 10_1101-2021_01_08_425864 388 31 leaving leave VBG 10_1101-2021_01_08_425864 388 32 the the DT 10_1101-2021_01_08_425864 388 33 parasite parasite NN 10_1101-2021_01_08_425864 388 34 exposed expose VBN 10_1101-2021_01_08_425864 388 35 to to IN 10_1101-2021_01_08_425864 388 36 an an DT 10_1101-2021_01_08_425864 388 37 environment environment NN 10_1101-2021_01_08_425864 388 38 rich rich JJ 10_1101-2021_01_08_425864 388 39 in in IN 10_1101-2021_01_08_425864 388 40 amino amino NN 10_1101-2021_01_08_425864 388 41 and and CC 10_1101-2021_01_08_425864 388 42 fatty fatty NN 10_1101-2021_01_08_425864 388 43 acids acid NNS 10_1101-2021_01_08_425864 388 44 in in IN 10_1101-2021_01_08_425864 388 45 596 596 CD 10_1101-2021_01_08_425864 388 46 the the DT 10_1101-2021_01_08_425864 388 47 digestive digestive JJ 10_1101-2021_01_08_425864 388 48 tube tube NN 10_1101-2021_01_08_425864 388 49 of of IN 10_1101-2021_01_08_425864 388 50 Rhodnius Rhodnius NNP 10_1101-2021_01_08_425864 388 51 prolixus prolixus NN 10_1101-2021_01_08_425864 388 52 [ [ -LRB- 10_1101-2021_01_08_425864 388 53 35,36 35,36 CD 10_1101-2021_01_08_425864 388 54 ] ] -RRB- 10_1101-2021_01_08_425864 388 55 . . . 10_1101-2021_01_08_425864 389 1 Because because IN 10_1101-2021_01_08_425864 389 2 the the DT 10_1101-2021_01_08_425864 389 3 digestive digestive JJ 10_1101-2021_01_08_425864 389 4 tract tract NN 10_1101-2021_01_08_425864 389 5 of of IN 10_1101-2021_01_08_425864 389 6 triatomine triatomine JJ 10_1101-2021_01_08_425864 389 7 insects insect NNS 10_1101-2021_01_08_425864 389 8 597 597 CD 10_1101-2021_01_08_425864 389 9 possesses possesse NNS 10_1101-2021_01_08_425864 389 10 a a DT 10_1101-2021_01_08_425864 389 11 perimicrovillar perimicrovillar JJ 10_1101-2021_01_08_425864 389 12 membrane membrane NN 10_1101-2021_01_08_425864 389 13 , , , 10_1101-2021_01_08_425864 389 14 which which WDT 10_1101-2021_01_08_425864 389 15 is be VBZ 10_1101-2021_01_08_425864 389 16 composed compose VBN 10_1101-2021_01_08_425864 389 17 primarily primarily RB 10_1101-2021_01_08_425864 389 18 of of IN 10_1101-2021_01_08_425864 389 19 lipids lipid NNS 10_1101-2021_01_08_425864 389 20 and and CC 10_1101-2021_01_08_425864 389 21 is be VBZ 10_1101-2021_01_08_425864 389 22 enriched enrich VBN 10_1101-2021_01_08_425864 389 23 by by IN 10_1101-2021_01_08_425864 389 24 598 598 CD 10_1101-2021_01_08_425864 389 25 glycoproteins glycoprotein NNS 10_1101-2021_01_08_425864 389 26 [ [ -LRB- 10_1101-2021_01_08_425864 389 27 37 37 CD 10_1101-2021_01_08_425864 389 28 ] ] -RRB- 10_1101-2021_01_08_425864 389 29 , , , 10_1101-2021_01_08_425864 389 30 it -PRON- PRP 10_1101-2021_01_08_425864 389 31 has have VBZ 10_1101-2021_01_08_425864 389 32 been be VBN 10_1101-2021_01_08_425864 389 33 speculated speculate VBN 10_1101-2021_01_08_425864 389 34 that that IN 10_1101-2021_01_08_425864 389 35 its -PRON- PRP$ 10_1101-2021_01_08_425864 389 36 degradation degradation NN 10_1101-2021_01_08_425864 389 37 could could MD 10_1101-2021_01_08_425864 389 38 provide provide VB 10_1101-2021_01_08_425864 389 39 lipids lipid NNS 10_1101-2021_01_08_425864 389 40 for for IN 10_1101-2021_01_08_425864 389 41 parasite parasite NN 10_1101-2021_01_08_425864 389 42 599 599 CD 10_1101-2021_01_08_425864 389 43 metabolism metabolism NN 10_1101-2021_01_08_425864 389 44 [ [ -LRB- 10_1101-2021_01_08_425864 389 45 38 38 CD 10_1101-2021_01_08_425864 389 46 ] ] -RRB- 10_1101-2021_01_08_425864 389 47 . . . 10_1101-2021_01_08_425864 390 1 In in IN 10_1101-2021_01_08_425864 390 2 this this DT 10_1101-2021_01_08_425864 390 3 study study NN 10_1101-2021_01_08_425864 390 4 , , , 10_1101-2021_01_08_425864 390 5 we -PRON- PRP 10_1101-2021_01_08_425864 390 6 showed show VBD 10_1101-2021_01_08_425864 390 7 that that IN 10_1101-2021_01_08_425864 390 8 the the DT 10_1101-2021_01_08_425864 390 9 insect insect NN 10_1101-2021_01_08_425864 390 10 stages stage NNS 10_1101-2021_01_08_425864 390 11 of of IN 10_1101-2021_01_08_425864 390 12 T. t. NN 10_1101-2021_01_08_425864 390 13 cruzi cruzi NNS 10_1101-2021_01_08_425864 390 14 coordinate coordinate VBP 10_1101-2021_01_08_425864 390 15 the the DT 10_1101-2021_01_08_425864 390 16 activation activation NN 10_1101-2021_01_08_425864 390 17 600 600 CD 10_1101-2021_01_08_425864 390 18 of of IN 10_1101-2021_01_08_425864 390 19 fatty fatty NN 10_1101-2021_01_08_425864 390 20 acid acid NN 10_1101-2021_01_08_425864 390 21 consumption consumption NN 10_1101-2021_01_08_425864 390 22 with with IN 10_1101-2021_01_08_425864 390 23 the the DT 10_1101-2021_01_08_425864 390 24 metabolism metabolism NN 10_1101-2021_01_08_425864 390 25 of of IN 10_1101-2021_01_08_425864 390 26 glucose glucose NN 10_1101-2021_01_08_425864 390 27 . . . 10_1101-2021_01_08_425864 391 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 391 2 experiments experiment NNS 10_1101-2021_01_08_425864 391 3 corroborate corroborate VBP 10_1101-2021_01_08_425864 391 4 early early JJ 10_1101-2021_01_08_425864 391 5 studies study NNS 10_1101-2021_01_08_425864 391 6 601 601 CD 10_1101-2021_01_08_425864 391 7 about about IN 10_1101-2021_01_08_425864 391 8 the the DT 10_1101-2021_01_08_425864 391 9 relatively relatively RB 10_1101-2021_01_08_425864 391 10 slow slow JJ 10_1101-2021_01_08_425864 391 11 use use NN 10_1101-2021_01_08_425864 391 12 of of IN 10_1101-2021_01_08_425864 391 13 palmitate palmitate NN 10_1101-2021_01_08_425864 391 14 as as IN 10_1101-2021_01_08_425864 391 15 an an DT 10_1101-2021_01_08_425864 391 16 energy energy NN 10_1101-2021_01_08_425864 391 17 source source NN 10_1101-2021_01_08_425864 391 18 by by IN 10_1101-2021_01_08_425864 391 19 proliferating proliferate VBG 10_1101-2021_01_08_425864 391 20 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 391 21 [ [ -LRB- 10_1101-2021_01_08_425864 391 22 39,40 39,40 CD 10_1101-2021_01_08_425864 391 23 ] ] -RRB- 10_1101-2021_01_08_425864 391 24 . . . 10_1101-2021_01_08_425864 392 1 602 602 CD 10_1101-2021_01_08_425864 392 2 In in IN 10_1101-2021_01_08_425864 392 3 addition addition NN 10_1101-2021_01_08_425864 392 4 , , , 10_1101-2021_01_08_425864 392 5 our -PRON- PRP$ 10_1101-2021_01_08_425864 392 6 results result NNS 10_1101-2021_01_08_425864 392 7 shed shed VBP 10_1101-2021_01_08_425864 392 8 light light NN 10_1101-2021_01_08_425864 392 9 on on IN 10_1101-2021_01_08_425864 392 10 the the DT 10_1101-2021_01_08_425864 392 11 end end NN 10_1101-2021_01_08_425864 392 12 product product NN 10_1101-2021_01_08_425864 392 13 excretion excretion NN 10_1101-2021_01_08_425864 392 14 by by IN 10_1101-2021_01_08_425864 392 15 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 392 16 forms form NNS 10_1101-2021_01_08_425864 392 17 during during IN 10_1101-2021_01_08_425864 392 18 603 603 CD 10_1101-2021_01_08_425864 392 19 incubation incubation NN 10_1101-2021_01_08_425864 392 20 under under IN 10_1101-2021_01_08_425864 392 21 starvation starvation NN 10_1101-2021_01_08_425864 392 22 conditions condition NNS 10_1101-2021_01_08_425864 392 23 , , , 10_1101-2021_01_08_425864 392 24 and and CC 10_1101-2021_01_08_425864 392 25 during during IN 10_1101-2021_01_08_425864 392 26 their -PRON- PRP$ 10_1101-2021_01_08_425864 392 27 recovery recovery NN 10_1101-2021_01_08_425864 392 28 from from IN 10_1101-2021_01_08_425864 392 29 starvation starvation NN 10_1101-2021_01_08_425864 392 30 using use VBG 10_1101-2021_01_08_425864 392 31 glucose glucose NN 10_1101-2021_01_08_425864 392 32 or or CC 10_1101-2021_01_08_425864 392 33 604 604 CD 10_1101-2021_01_08_425864 392 34 palmitate palmitate NN 10_1101-2021_01_08_425864 392 35 . . . 10_1101-2021_01_08_425864 393 1 First first RB 10_1101-2021_01_08_425864 393 2 , , , 10_1101-2021_01_08_425864 393 3 we -PRON- PRP 10_1101-2021_01_08_425864 393 4 showed show VBD 10_1101-2021_01_08_425864 393 5 that that IN 10_1101-2021_01_08_425864 393 6 non non JJ 10_1101-2021_01_08_425864 393 7 - - JJ 10_1101-2021_01_08_425864 393 8 starved starved JJ 10_1101-2021_01_08_425864 393 9 and and CC 10_1101-2021_01_08_425864 393 10 starved starved JJ 10_1101-2021_01_08_425864 393 11 parasites parasite NNS 10_1101-2021_01_08_425864 393 12 recovered recover VBN 10_1101-2021_01_08_425864 393 13 in in IN 10_1101-2021_01_08_425864 393 14 the the DT 10_1101-2021_01_08_425864 393 15 presence presence NN 10_1101-2021_01_08_425864 393 16 of of IN 10_1101-2021_01_08_425864 393 17 605 605 CD 10_1101-2021_01_08_425864 393 18 glucose glucose NN 10_1101-2021_01_08_425864 393 19 , , , 10_1101-2021_01_08_425864 393 20 excreting excrete VBG 10_1101-2021_01_08_425864 393 21 succinate succinate NN 10_1101-2021_01_08_425864 393 22 as as IN 10_1101-2021_01_08_425864 393 23 their -PRON- PRP$ 10_1101-2021_01_08_425864 393 24 primary primary JJ 10_1101-2021_01_08_425864 393 25 metabolic metabolic JJ 10_1101-2021_01_08_425864 393 26 waste waste NN 10_1101-2021_01_08_425864 393 27 , , , 10_1101-2021_01_08_425864 393 28 as as IN 10_1101-2021_01_08_425864 393 29 expected expect VBN 10_1101-2021_01_08_425864 393 30 [ [ -LRB- 10_1101-2021_01_08_425864 393 31 41–43 41–43 CD 10_1101-2021_01_08_425864 393 32 ] ] -RRB- 10_1101-2021_01_08_425864 393 33 . . . 10_1101-2021_01_08_425864 394 1 After after IN 10_1101-2021_01_08_425864 394 2 16 16 CD 10_1101-2021_01_08_425864 394 3 h h NN 10_1101-2021_01_08_425864 394 4 of of IN 10_1101-2021_01_08_425864 394 5 606 606 CD 10_1101-2021_01_08_425864 394 6 nutritional nutritional JJ 10_1101-2021_01_08_425864 394 7 starvation starvation NN 10_1101-2021_01_08_425864 394 8 , , , 10_1101-2021_01_08_425864 394 9 the the DT 10_1101-2021_01_08_425864 394 10 consumption consumption NN 10_1101-2021_01_08_425864 394 11 of of IN 10_1101-2021_01_08_425864 394 12 internal internal JJ 10_1101-2021_01_08_425864 394 13 carbon carbon NN 10_1101-2021_01_08_425864 394 14 sources source NNS 10_1101-2021_01_08_425864 394 15 produces produce VBZ 10_1101-2021_01_08_425864 394 16 acetate acetate RB 10_1101-2021_01_08_425864 394 17 as as IN 10_1101-2021_01_08_425864 394 18 the the DT 10_1101-2021_01_08_425864 394 19 primary primary JJ 10_1101-2021_01_08_425864 394 20 607 607 CD 10_1101-2021_01_08_425864 394 21 end end NN 10_1101-2021_01_08_425864 394 22 - - HYPH 10_1101-2021_01_08_425864 394 23 product product NN 10_1101-2021_01_08_425864 394 24 . . . 10_1101-2021_01_08_425864 395 1 In in IN 10_1101-2021_01_08_425864 395 2 the the DT 10_1101-2021_01_08_425864 395 3 presence presence NN 10_1101-2021_01_08_425864 395 4 of of IN 10_1101-2021_01_08_425864 395 5 glucose glucose NN 10_1101-2021_01_08_425864 395 6 after after IN 10_1101-2021_01_08_425864 395 7 16 16 CD 10_1101-2021_01_08_425864 395 8 h h NN 10_1101-2021_01_08_425864 395 9 of of IN 10_1101-2021_01_08_425864 395 10 starvation starvation NN 10_1101-2021_01_08_425864 395 11 , , , 10_1101-2021_01_08_425864 395 12 we -PRON- PRP 10_1101-2021_01_08_425864 395 13 found find VBD 10_1101-2021_01_08_425864 395 14 that that IN 10_1101-2021_01_08_425864 395 15 glucose glucose NN 10_1101-2021_01_08_425864 395 16 - - HYPH 10_1101-2021_01_08_425864 395 17 derived derive VBN 10_1101-2021_01_08_425864 395 18 608 608 CD 10_1101-2021_01_08_425864 395 19 carbons carbon NNS 10_1101-2021_01_08_425864 395 20 contribute contribute VBP 10_1101-2021_01_08_425864 395 21 to to IN 10_1101-2021_01_08_425864 395 22 the the DT 10_1101-2021_01_08_425864 395 23 excreted excreted JJ 10_1101-2021_01_08_425864 395 24 pools pool NNS 10_1101-2021_01_08_425864 395 25 of of IN 10_1101-2021_01_08_425864 395 26 acetate acetate NNP 10_1101-2021_01_08_425864 395 27 and and CC 10_1101-2021_01_08_425864 395 28 lactate lactate JJ 10_1101-2021_01_08_425864 395 29 . . . 10_1101-2021_01_08_425864 396 1 Interestingly interestingly RB 10_1101-2021_01_08_425864 396 2 , , , 10_1101-2021_01_08_425864 396 3 palmitate palmitate NN 10_1101-2021_01_08_425864 396 4 metabolism metabolism NN 10_1101-2021_01_08_425864 396 5 609 609 CD 10_1101-2021_01_08_425864 396 6 contributed contribute VBD 10_1101-2021_01_08_425864 396 7 to to IN 10_1101-2021_01_08_425864 396 8 the the DT 10_1101-2021_01_08_425864 396 9 increase increase NN 10_1101-2021_01_08_425864 396 10 in in IN 10_1101-2021_01_08_425864 396 11 acetate acetate JJ 10_1101-2021_01_08_425864 396 12 production production NN 10_1101-2021_01_08_425864 396 13 , , , 10_1101-2021_01_08_425864 396 14 followed follow VBN 10_1101-2021_01_08_425864 396 15 by by IN 10_1101-2021_01_08_425864 396 16 the the DT 10_1101-2021_01_08_425864 396 17 production production NN 10_1101-2021_01_08_425864 396 18 of of IN 10_1101-2021_01_08_425864 396 19 alanine alanine NN 10_1101-2021_01_08_425864 396 20 , , , 10_1101-2021_01_08_425864 396 21 pyruvate pyruvate NNP 10_1101-2021_01_08_425864 396 22 , , , 10_1101-2021_01_08_425864 396 23 610 610 CD 10_1101-2021_01_08_425864 396 24 succinate succinate NN 10_1101-2021_01_08_425864 396 25 and and CC 10_1101-2021_01_08_425864 396 26 lactate lactate VBP 10_1101-2021_01_08_425864 396 27 . . . 10_1101-2021_01_08_425864 397 1 The the DT 10_1101-2021_01_08_425864 397 2 unexpected unexpected JJ 10_1101-2021_01_08_425864 397 3 production production NN 10_1101-2021_01_08_425864 397 4 of of IN 10_1101-2021_01_08_425864 397 5 alanine alanine NN 10_1101-2021_01_08_425864 397 6 , , , 10_1101-2021_01_08_425864 397 7 pyruvate pyruvate VB 10_1101-2021_01_08_425864 397 8 and and CC 10_1101-2021_01_08_425864 397 9 lactate lactate JJ 10_1101-2021_01_08_425864 397 10 can can MD 10_1101-2021_01_08_425864 397 11 be be VB 10_1101-2021_01_08_425864 397 12 explained explain VBN 10_1101-2021_01_08_425864 397 13 611 611 CD 10_1101-2021_01_08_425864 397 14 by by IN 10_1101-2021_01_08_425864 397 15 an an DT 10_1101-2021_01_08_425864 397 16 increase increase NN 10_1101-2021_01_08_425864 397 17 in in IN 10_1101-2021_01_08_425864 397 18 the the DT 10_1101-2021_01_08_425864 397 19 TCA TCA NNP 10_1101-2021_01_08_425864 397 20 cycle cycle NN 10_1101-2021_01_08_425864 397 21 activity activity NN 10_1101-2021_01_08_425864 397 22 , , , 10_1101-2021_01_08_425864 397 23 producing produce VBG 10_1101-2021_01_08_425864 397 24 malate malate NN 10_1101-2021_01_08_425864 397 25 , , , 10_1101-2021_01_08_425864 397 26 which which WDT 10_1101-2021_01_08_425864 397 27 can can MD 10_1101-2021_01_08_425864 397 28 be be VB 10_1101-2021_01_08_425864 397 29 converted convert VBN 10_1101-2021_01_08_425864 397 30 into into IN 10_1101-2021_01_08_425864 397 31 pyruvate pyruvate NN 10_1101-2021_01_08_425864 397 32 by by IN 10_1101-2021_01_08_425864 397 33 612 612 CD 10_1101-2021_01_08_425864 397 34 the the DT 10_1101-2021_01_08_425864 397 35 decarboxylative decarboxylative JJ 10_1101-2021_01_08_425864 397 36 reaction reaction NN 10_1101-2021_01_08_425864 397 37 of of IN 10_1101-2021_01_08_425864 397 38 the the DT 10_1101-2021_01_08_425864 397 39 malic malic JJ 10_1101-2021_01_08_425864 397 40 enzyme enzyme NNS 10_1101-2021_01_08_425864 397 41 ( ( -LRB- 10_1101-2021_01_08_425864 397 42 ME me NN 10_1101-2021_01_08_425864 397 43 ) ) -RRB- 10_1101-2021_01_08_425864 397 44 [ [ -LRB- 10_1101-2021_01_08_425864 397 45 44 44 CD 10_1101-2021_01_08_425864 397 46 ] ] -RRB- 10_1101-2021_01_08_425864 397 47 . . . 10_1101-2021_01_08_425864 398 1 Pyruvate Pyruvate NNP 10_1101-2021_01_08_425864 398 2 can can MD 10_1101-2021_01_08_425864 398 3 be be VB 10_1101-2021_01_08_425864 398 4 converted convert VBN 10_1101-2021_01_08_425864 398 5 into into IN 10_1101-2021_01_08_425864 398 6 alanine alanine NN 10_1101-2021_01_08_425864 398 7 613 613 CD 10_1101-2021_01_08_425864 398 8 through through IN 10_1101-2021_01_08_425864 398 9 a a DT 10_1101-2021_01_08_425864 398 10 transamination transamination NN 10_1101-2021_01_08_425864 398 11 reaction reaction NN 10_1101-2021_01_08_425864 398 12 by by IN 10_1101-2021_01_08_425864 398 13 an an DT 10_1101-2021_01_08_425864 398 14 alanine- alanine- JJ 10_1101-2021_01_08_425864 398 15 [ [ -LRB- 10_1101-2021_01_08_425864 398 16 45 45 CD 10_1101-2021_01_08_425864 398 17 ] ] -RRB- 10_1101-2021_01_08_425864 398 18 , , , 10_1101-2021_01_08_425864 398 19 a a DT 10_1101-2021_01_08_425864 398 20 tyrosine- tyrosine- JJ 10_1101-2021_01_08_425864 398 21 [ [ -LRB- 10_1101-2021_01_08_425864 398 22 46 46 CD 10_1101-2021_01_08_425864 398 23 ] ] -RRB- 10_1101-2021_01_08_425864 398 24 an an DT 10_1101-2021_01_08_425864 398 25 aspartate aspartate NN 10_1101-2021_01_08_425864 398 26 aminotransferase aminotransferase NN 10_1101-2021_01_08_425864 398 27 614 614 CD 10_1101-2021_01_08_425864 398 28 [ [ -LRB- 10_1101-2021_01_08_425864 398 29 47 47 CD 10_1101-2021_01_08_425864 398 30 ] ] -RRB- 10_1101-2021_01_08_425864 398 31 , , , 10_1101-2021_01_08_425864 398 32 or or CC 10_1101-2021_01_08_425864 398 33 a a DT 10_1101-2021_01_08_425864 398 34 reductive reductive JJ 10_1101-2021_01_08_425864 398 35 amination amination NN 10_1101-2021_01_08_425864 398 36 by by IN 10_1101-2021_01_08_425864 398 37 an an DT 10_1101-2021_01_08_425864 398 38 alanine alanine NN 10_1101-2021_01_08_425864 398 39 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 398 40 [ [ -LRB- 10_1101-2021_01_08_425864 398 41 48 48 CD 10_1101-2021_01_08_425864 398 42 ] ] -RRB- 10_1101-2021_01_08_425864 398 43 . . . 10_1101-2021_01_08_425864 399 1 The the DT 10_1101-2021_01_08_425864 399 2 excretion excretion NN 10_1101-2021_01_08_425864 399 3 of of IN 10_1101-2021_01_08_425864 399 4 lactate lactate NN 10_1101-2021_01_08_425864 399 5 could could MD 10_1101-2021_01_08_425864 399 6 be be VB 10_1101-2021_01_08_425864 399 7 a a DT 10_1101-2021_01_08_425864 399 8 615 615 CD 10_1101-2021_01_08_425864 399 9 consequence consequence NN 10_1101-2021_01_08_425864 399 10 of of IN 10_1101-2021_01_08_425864 399 11 lactate lactate JJ 10_1101-2021_01_08_425864 399 12 dehydrogenase dehydrogenase JJ 10_1101-2021_01_08_425864 399 13 activity activity NN 10_1101-2021_01_08_425864 399 14 . . . 10_1101-2021_01_08_425864 400 1 However however RB 10_1101-2021_01_08_425864 400 2 , , , 10_1101-2021_01_08_425864 400 3 it -PRON- PRP 10_1101-2021_01_08_425864 400 4 should should MD 10_1101-2021_01_08_425864 400 5 be be VB 10_1101-2021_01_08_425864 400 6 noted note VBN 10_1101-2021_01_08_425864 400 7 that that IN 10_1101-2021_01_08_425864 400 8 this this DT 10_1101-2021_01_08_425864 400 9 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 400 10 616 616 CD 10_1101-2021_01_08_425864 400 11 activity activity NN 10_1101-2021_01_08_425864 400 12 has have VBZ 10_1101-2021_01_08_425864 400 13 not not RB 10_1101-2021_01_08_425864 400 14 been be VBN 10_1101-2021_01_08_425864 400 15 observed observe VBN 10_1101-2021_01_08_425864 400 16 to to IN 10_1101-2021_01_08_425864 400 17 date date NN 10_1101-2021_01_08_425864 400 18 . . . 10_1101-2021_01_08_425864 401 1 In in IN 10_1101-2021_01_08_425864 401 2 relation relation NN 10_1101-2021_01_08_425864 401 3 to to IN 10_1101-2021_01_08_425864 401 4 the the DT 10_1101-2021_01_08_425864 401 5 succinate succinate NN 10_1101-2021_01_08_425864 401 6 production production NN 10_1101-2021_01_08_425864 401 7 , , , 10_1101-2021_01_08_425864 401 8 a a DT 10_1101-2021_01_08_425864 401 9 relevant relevant JJ 10_1101-2021_01_08_425864 401 10 factor factor NN 10_1101-2021_01_08_425864 401 11 617 617 CD 10_1101-2021_01_08_425864 401 12 favouring favour VBG 10_1101-2021_01_08_425864 401 13 this this DT 10_1101-2021_01_08_425864 401 14 process process NN 10_1101-2021_01_08_425864 401 15 is be VBZ 10_1101-2021_01_08_425864 401 16 the the DT 10_1101-2021_01_08_425864 401 17 production production NN 10_1101-2021_01_08_425864 401 18 of of IN 10_1101-2021_01_08_425864 401 19 NADH NADH NNP 10_1101-2021_01_08_425864 401 20 by by IN 10_1101-2021_01_08_425864 401 21 the the DT 10_1101-2021_01_08_425864 401 22 third third JJ 10_1101-2021_01_08_425864 401 23 step step NN 10_1101-2021_01_08_425864 401 24 of of IN 10_1101-2021_01_08_425864 401 25 the the DT 10_1101-2021_01_08_425864 401 26 beta beta NN 10_1101-2021_01_08_425864 401 27 - - HYPH 10_1101-2021_01_08_425864 401 28 oxidation oxidation NN 10_1101-2021_01_08_425864 401 29 ( ( -LRB- 10_1101-2021_01_08_425864 401 30 3- 3- NN 10_1101-2021_01_08_425864 401 31 618 618 CD 10_1101-2021_01_08_425864 401 32 hydroxyacyl hydroxyacyl NN 10_1101-2021_01_08_425864 401 33 - - HYPH 10_1101-2021_01_08_425864 401 34 CoA CoA NNP 10_1101-2021_01_08_425864 401 35 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 401 36 ) ) -RRB- 10_1101-2021_01_08_425864 401 37 . . . 10_1101-2021_01_08_425864 402 1 This this DT 10_1101-2021_01_08_425864 402 2 NADH NADH NNP 10_1101-2021_01_08_425864 402 3 can can MD 10_1101-2021_01_08_425864 402 4 be be VB 10_1101-2021_01_08_425864 402 5 oxidized oxidize VBN 10_1101-2021_01_08_425864 402 6 through through IN 10_1101-2021_01_08_425864 402 7 the the DT 10_1101-2021_01_08_425864 402 8 activity activity NN 10_1101-2021_01_08_425864 402 9 of of IN 10_1101-2021_01_08_425864 402 10 NADH- NADH- NNP 10_1101-2021_01_08_425864 402 11 .CC .CC NFP 10_1101-2021_01_08_425864 402 12 - - : 10_1101-2021_01_08_425864 402 13 BY by IN 10_1101-2021_01_08_425864 402 14 4.0 4.0 CD 10_1101-2021_01_08_425864 402 15 International international JJ 10_1101-2021_01_08_425864 402 16 licensemade licensemade NN 10_1101-2021_01_08_425864 402 17 available available JJ 10_1101-2021_01_08_425864 402 18 under under IN 10_1101-2021_01_08_425864 402 19 a a DT 10_1101-2021_01_08_425864 402 20 ( ( -LRB- 10_1101-2021_01_08_425864 402 21 which which WDT 10_1101-2021_01_08_425864 402 22 was be VBD 10_1101-2021_01_08_425864 402 23 not not RB 10_1101-2021_01_08_425864 402 24 certified certify VBN 10_1101-2021_01_08_425864 402 25 by by IN 10_1101-2021_01_08_425864 402 26 peer peer NN 10_1101-2021_01_08_425864 402 27 review review NN 10_1101-2021_01_08_425864 402 28 ) ) -RRB- 10_1101-2021_01_08_425864 402 29 is be VBZ 10_1101-2021_01_08_425864 402 30 the the DT 10_1101-2021_01_08_425864 402 31 author author NN 10_1101-2021_01_08_425864 402 32 / / SYM 10_1101-2021_01_08_425864 402 33 funder funder NN 10_1101-2021_01_08_425864 402 34 , , , 10_1101-2021_01_08_425864 402 35 who who WP 10_1101-2021_01_08_425864 402 36 has have VBZ 10_1101-2021_01_08_425864 402 37 granted grant VBN 10_1101-2021_01_08_425864 402 38 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 402 39 a a DT 10_1101-2021_01_08_425864 402 40 license license NN 10_1101-2021_01_08_425864 402 41 to to TO 10_1101-2021_01_08_425864 402 42 display display VB 10_1101-2021_01_08_425864 402 43 the the DT 10_1101-2021_01_08_425864 402 44 preprint preprint NN 10_1101-2021_01_08_425864 402 45 in in IN 10_1101-2021_01_08_425864 402 46 perpetuity perpetuity NN 10_1101-2021_01_08_425864 402 47 . . . 10_1101-2021_01_08_425864 403 1 It -PRON- PRP 10_1101-2021_01_08_425864 403 2 is be VBZ 10_1101-2021_01_08_425864 403 3 The the DT 10_1101-2021_01_08_425864 403 4 copyright copyright NN 10_1101-2021_01_08_425864 403 5 holder holder NN 10_1101-2021_01_08_425864 403 6 for for IN 10_1101-2021_01_08_425864 403 7 this this DT 10_1101-2021_01_08_425864 403 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 403 9 version version NN 10_1101-2021_01_08_425864 403 10 posted post VBD 10_1101-2021_01_08_425864 403 11 January January NNP 10_1101-2021_01_08_425864 403 12 8 8 CD 10_1101-2021_01_08_425864 403 13 , , , 10_1101-2021_01_08_425864 403 14 2021 2021 CD 10_1101-2021_01_08_425864 403 15 . . . 10_1101-2021_01_08_425864 403 16 ; ; : 10_1101-2021_01_08_425864 403 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 403 18 : : : 10_1101-2021_01_08_425864 403 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 403 20 preprint preprint NN 10_1101-2021_01_08_425864 403 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 403 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ VBZ 10_1101-2021_01_08_425864 403 23 619 619 CD 10_1101-2021_01_08_425864 403 24 dependent dependent JJ 10_1101-2021_01_08_425864 403 25 mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 403 26 fumarate fumarate NN 10_1101-2021_01_08_425864 403 27 reductase reductase NN 10_1101-2021_01_08_425864 403 28 [ [ -LRB- 10_1101-2021_01_08_425864 403 29 49 49 CD 10_1101-2021_01_08_425864 403 30 ] ] -RRB- 10_1101-2021_01_08_425864 403 31 , , , 10_1101-2021_01_08_425864 403 32 which which WDT 10_1101-2021_01_08_425864 403 33 concomitantly concomitantly RB 10_1101-2021_01_08_425864 403 34 converts convert VBZ 10_1101-2021_01_08_425864 403 35 NADH NADH NNP 10_1101-2021_01_08_425864 403 36 into into IN 10_1101-2021_01_08_425864 403 37 NAD+ NAD+ NNP 10_1101-2021_01_08_425864 403 38 620 620 CD 10_1101-2021_01_08_425864 403 39 and and CC 10_1101-2021_01_08_425864 403 40 fumarate fumarate VBP 10_1101-2021_01_08_425864 403 41 into into IN 10_1101-2021_01_08_425864 403 42 succinate succinate NN 10_1101-2021_01_08_425864 403 43 . . . 10_1101-2021_01_08_425864 404 1 This this DT 10_1101-2021_01_08_425864 404 2 succinate succinate NN 10_1101-2021_01_08_425864 404 3 can can MD 10_1101-2021_01_08_425864 404 4 be be VB 10_1101-2021_01_08_425864 404 5 excreted excrete VBN 10_1101-2021_01_08_425864 404 6 or or CC 10_1101-2021_01_08_425864 404 7 re re VBN 10_1101-2021_01_08_425864 404 8 - - VBN 10_1101-2021_01_08_425864 404 9 used use VBN 10_1101-2021_01_08_425864 404 10 by by IN 10_1101-2021_01_08_425864 404 11 the the DT 10_1101-2021_01_08_425864 404 12 TCA TCA NNP 10_1101-2021_01_08_425864 404 13 cycle cycle NN 10_1101-2021_01_08_425864 404 14 , , , 10_1101-2021_01_08_425864 404 15 and and CC 10_1101-2021_01_08_425864 404 16 the the DT 10_1101-2021_01_08_425864 404 17 621 621 CD 10_1101-2021_01_08_425864 404 18 resulting result VBG 10_1101-2021_01_08_425864 404 19 NAD+ NAD+ NNP 10_1101-2021_01_08_425864 404 20 can can MD 10_1101-2021_01_08_425864 404 21 be be VB 10_1101-2021_01_08_425864 404 22 used use VBN 10_1101-2021_01_08_425864 404 23 as as IN 10_1101-2021_01_08_425864 404 24 a a DT 10_1101-2021_01_08_425864 404 25 cofactor cofactor NN 10_1101-2021_01_08_425864 404 26 for for IN 10_1101-2021_01_08_425864 404 27 other other JJ 10_1101-2021_01_08_425864 404 28 enzymes enzyme NNS 10_1101-2021_01_08_425864 404 29 . . . 10_1101-2021_01_08_425864 405 1 622 622 CD 10_1101-2021_01_08_425864 405 2 As as IN 10_1101-2021_01_08_425864 405 3 previously previously RB 10_1101-2021_01_08_425864 405 4 mentioned mention VBN 10_1101-2021_01_08_425864 405 5 , , , 10_1101-2021_01_08_425864 405 6 it -PRON- PRP 10_1101-2021_01_08_425864 405 7 is be VBZ 10_1101-2021_01_08_425864 405 8 well well RB 10_1101-2021_01_08_425864 405 9 known know VBN 10_1101-2021_01_08_425864 405 10 that that IN 10_1101-2021_01_08_425864 405 11 during during IN 10_1101-2021_01_08_425864 405 12 the the DT 10_1101-2021_01_08_425864 405 13 initial initial JJ 10_1101-2021_01_08_425864 405 14 phase phase NN 10_1101-2021_01_08_425864 405 15 of of IN 10_1101-2021_01_08_425864 405 16 proliferation proliferation NN 10_1101-2021_01_08_425864 405 17 , , , 10_1101-2021_01_08_425864 405 18 623 623 CD 10_1101-2021_01_08_425864 405 19 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 405 20 preferentially preferentially RB 10_1101-2021_01_08_425864 405 21 consume consume VBP 10_1101-2021_01_08_425864 405 22 glucose glucose NN 10_1101-2021_01_08_425864 405 23 , , , 10_1101-2021_01_08_425864 405 24 and and CC 10_1101-2021_01_08_425864 405 25 during during IN 10_1101-2021_01_08_425864 405 26 the the DT 10_1101-2021_01_08_425864 405 27 stationary stationary JJ 10_1101-2021_01_08_425864 405 28 phase phase NN 10_1101-2021_01_08_425864 405 29 , , , 10_1101-2021_01_08_425864 405 30 a a DT 10_1101-2021_01_08_425864 405 31 metabolic metabolic JJ 10_1101-2021_01_08_425864 405 32 switch switch NN 10_1101-2021_01_08_425864 405 33 624 624 CD 10_1101-2021_01_08_425864 405 34 occurs occur VBZ 10_1101-2021_01_08_425864 405 35 towards towards IN 10_1101-2021_01_08_425864 405 36 the the DT 10_1101-2021_01_08_425864 405 37 consumption consumption NN 10_1101-2021_01_08_425864 405 38 of of IN 10_1101-2021_01_08_425864 405 39 amino amino JJ 10_1101-2021_01_08_425864 405 40 acids acid NNS 10_1101-2021_01_08_425864 405 41 [ [ -LRB- 10_1101-2021_01_08_425864 405 42 8,10,42 8,10,42 CD 10_1101-2021_01_08_425864 405 43 ] ] -RRB- 10_1101-2021_01_08_425864 405 44 . . . 10_1101-2021_01_08_425864 406 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 406 2 results result NNS 10_1101-2021_01_08_425864 406 3 show show VBP 10_1101-2021_01_08_425864 406 4 that that IN 10_1101-2021_01_08_425864 406 5 this this DT 10_1101-2021_01_08_425864 406 6 switch switch NN 10_1101-2021_01_08_425864 406 7 625 625 CD 10_1101-2021_01_08_425864 406 8 constitutes constitute VBZ 10_1101-2021_01_08_425864 406 9 a a DT 10_1101-2021_01_08_425864 406 10 broader broad JJR 10_1101-2021_01_08_425864 406 11 and and CC 10_1101-2021_01_08_425864 406 12 more more JJR 10_1101-2021_01_08_425864 406 13 systemic systemic JJ 10_1101-2021_01_08_425864 406 14 metabolic metabolic NN 10_1101-2021_01_08_425864 406 15 reprogramming reprogramming NN 10_1101-2021_01_08_425864 406 16 , , , 10_1101-2021_01_08_425864 406 17 which which WDT 10_1101-2021_01_08_425864 406 18 also also RB 10_1101-2021_01_08_425864 406 19 includes include VBZ 10_1101-2021_01_08_425864 406 20 FAO FAO NNP 10_1101-2021_01_08_425864 406 21 . . . 10_1101-2021_01_08_425864 407 1 We -PRON- PRP 10_1101-2021_01_08_425864 407 2 626 626 CD 10_1101-2021_01_08_425864 407 3 detected detect VBD 10_1101-2021_01_08_425864 407 4 this this DT 10_1101-2021_01_08_425864 407 5 switch switch NN 10_1101-2021_01_08_425864 407 6 through through IN 10_1101-2021_01_08_425864 407 7 changes change NNS 10_1101-2021_01_08_425864 407 8 in in IN 10_1101-2021_01_08_425864 407 9 the the DT 10_1101-2021_01_08_425864 407 10 enzymatic enzymatic JJ 10_1101-2021_01_08_425864 407 11 activities activity NNS 10_1101-2021_01_08_425864 407 12 of of IN 10_1101-2021_01_08_425864 407 13 key key JJ 10_1101-2021_01_08_425864 407 14 enzymes enzyme NNS 10_1101-2021_01_08_425864 407 15 responsible responsible JJ 10_1101-2021_01_08_425864 407 16 for for IN 10_1101-2021_01_08_425864 407 17 the the DT 10_1101-2021_01_08_425864 407 18 627 627 CD 10_1101-2021_01_08_425864 407 19 regulation regulation NN 10_1101-2021_01_08_425864 407 20 of of IN 10_1101-2021_01_08_425864 407 21 FAO FAO NNP 10_1101-2021_01_08_425864 407 22 , , , 10_1101-2021_01_08_425864 407 23 such such JJ 10_1101-2021_01_08_425864 407 24 as as IN 10_1101-2021_01_08_425864 407 25 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 407 26 and and CC 10_1101-2021_01_08_425864 407 27 ACC ACC NNP 10_1101-2021_01_08_425864 407 28 , , , 10_1101-2021_01_08_425864 407 29 which which WDT 10_1101-2021_01_08_425864 407 30 have have VBP 10_1101-2021_01_08_425864 407 31 increased increase VBN 10_1101-2021_01_08_425864 407 32 and and CC 10_1101-2021_01_08_425864 407 33 decreased decrease VBN 10_1101-2021_01_08_425864 407 34 activities activity NNS 10_1101-2021_01_08_425864 407 35 , , , 10_1101-2021_01_08_425864 407 36 628 628 CD 10_1101-2021_01_08_425864 407 37 respectively respectively RB 10_1101-2021_01_08_425864 407 38 , , , 10_1101-2021_01_08_425864 407 39 in in IN 10_1101-2021_01_08_425864 407 40 the the DT 10_1101-2021_01_08_425864 407 41 presence presence NN 10_1101-2021_01_08_425864 407 42 of of IN 10_1101-2021_01_08_425864 407 43 glucose glucose NN 10_1101-2021_01_08_425864 407 44 . . . 10_1101-2021_01_08_425864 408 1 Our -PRON- PRP$ 10_1101-2021_01_08_425864 408 2 findings finding NNS 10_1101-2021_01_08_425864 408 3 showed show VBD 10_1101-2021_01_08_425864 408 4 that that IN 10_1101-2021_01_08_425864 408 5 the the DT 10_1101-2021_01_08_425864 408 6 inhibition inhibition NN 10_1101-2021_01_08_425864 408 7 of of IN 10_1101-2021_01_08_425864 408 8 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 408 9 affects affect VBZ 10_1101-2021_01_08_425864 408 10 the the DT 10_1101-2021_01_08_425864 408 11 629 629 CD 10_1101-2021_01_08_425864 408 12 late late JJ 10_1101-2021_01_08_425864 408 13 phase phase NN 10_1101-2021_01_08_425864 408 14 of of IN 10_1101-2021_01_08_425864 408 15 proliferation proliferation NN 10_1101-2021_01_08_425864 408 16 of of IN 10_1101-2021_01_08_425864 408 17 epimastigotes epimastigotes NNPS 10_1101-2021_01_08_425864 408 18 when when WRB 10_1101-2021_01_08_425864 408 19 the the DT 10_1101-2021_01_08_425864 408 20 switch switch NN 10_1101-2021_01_08_425864 408 21 to to IN 10_1101-2021_01_08_425864 408 22 FAO FAO NNP 10_1101-2021_01_08_425864 408 23 has have VBZ 10_1101-2021_01_08_425864 408 24 already already RB 10_1101-2021_01_08_425864 408 25 occurred occur VBN 10_1101-2021_01_08_425864 408 26 . . . 10_1101-2021_01_08_425864 409 1 630 630 CD 10_1101-2021_01_08_425864 409 2 An an DT 10_1101-2021_01_08_425864 409 3 interesting interesting JJ 10_1101-2021_01_08_425864 409 4 question question NN 10_1101-2021_01_08_425864 409 5 about about RB 10_1101-2021_01_08_425864 409 6 T. t. NN 10_1101-2021_01_08_425864 409 7 cruzi cruzi NNS 10_1101-2021_01_08_425864 409 8 epimastigotes epimastigote VBZ 10_1101-2021_01_08_425864 409 9 is be VBZ 10_1101-2021_01_08_425864 409 10 how how WRB 10_1101-2021_01_08_425864 409 11 they -PRON- PRP 10_1101-2021_01_08_425864 409 12 survive survive VBP 10_1101-2021_01_08_425864 409 13 long long JJ 10_1101-2021_01_08_425864 409 14 periods period NNS 10_1101-2021_01_08_425864 409 15 of of IN 10_1101-2021_01_08_425864 409 16 631 631 CD 10_1101-2021_01_08_425864 409 17 starvation starvation NN 10_1101-2021_01_08_425864 409 18 . . . 10_1101-2021_01_08_425864 410 1 Early early JJ 10_1101-2021_01_08_425864 410 2 data datum NNS 10_1101-2021_01_08_425864 410 3 showed show VBD 10_1101-2021_01_08_425864 410 4 high high JJ 10_1101-2021_01_08_425864 410 5 respiration respiration NN 10_1101-2021_01_08_425864 410 6 levels level NNS 10_1101-2021_01_08_425864 410 7 in in IN 10_1101-2021_01_08_425864 410 8 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 410 9 incubated incubate VBN 10_1101-2021_01_08_425864 410 10 in in IN 10_1101-2021_01_08_425864 410 11 the the DT 10_1101-2021_01_08_425864 410 12 absence absence NN 10_1101-2021_01_08_425864 410 13 of of IN 10_1101-2021_01_08_425864 410 14 632 632 CD 10_1101-2021_01_08_425864 410 15 external external JJ 10_1101-2021_01_08_425864 410 16 oxidisable oxidisable JJ 10_1101-2021_01_08_425864 410 17 carbon carbon NN 10_1101-2021_01_08_425864 410 18 sources source NNS 10_1101-2021_01_08_425864 410 19 . . . 10_1101-2021_01_08_425864 411 1 This this DT 10_1101-2021_01_08_425864 411 2 oxygen oxygen NN 10_1101-2021_01_08_425864 411 3 consumption consumption NN 10_1101-2021_01_08_425864 411 4 was be VBD 10_1101-2021_01_08_425864 411 5 attributed attribute VBN 10_1101-2021_01_08_425864 411 6 to to IN 10_1101-2021_01_08_425864 411 7 the the DT 10_1101-2021_01_08_425864 411 8 breakdown breakdown NN 10_1101-2021_01_08_425864 411 9 of of IN 10_1101-2021_01_08_425864 411 10 633 633 CD 10_1101-2021_01_08_425864 411 11 TAGs tag NNS 10_1101-2021_01_08_425864 411 12 into into IN 10_1101-2021_01_08_425864 411 13 free free JJ 10_1101-2021_01_08_425864 411 14 fatty fatty NN 10_1101-2021_01_08_425864 411 15 acids acid NNS 10_1101-2021_01_08_425864 411 16 and and CC 10_1101-2021_01_08_425864 411 17 their -PRON- PRP$ 10_1101-2021_01_08_425864 411 18 further further JJ 10_1101-2021_01_08_425864 411 19 oxidation oxidation NN 10_1101-2021_01_08_425864 411 20 [ [ -LRB- 10_1101-2021_01_08_425864 411 21 50 50 CD 10_1101-2021_01_08_425864 411 22 ] ] -RRB- 10_1101-2021_01_08_425864 411 23 . . . 10_1101-2021_01_08_425864 412 1 Here here RB 10_1101-2021_01_08_425864 412 2 , , , 10_1101-2021_01_08_425864 412 3 we -PRON- PRP 10_1101-2021_01_08_425864 412 4 confirmed confirm VBD 10_1101-2021_01_08_425864 412 5 this this DT 10_1101-2021_01_08_425864 412 6 finding finding NN 10_1101-2021_01_08_425864 412 7 by by IN 10_1101-2021_01_08_425864 412 8 634 634 CD 10_1101-2021_01_08_425864 412 9 inhibiting inhibit VBG 10_1101-2021_01_08_425864 412 10 the the DT 10_1101-2021_01_08_425864 412 11 internal internal JJ 10_1101-2021_01_08_425864 412 12 fatty fatty NN 10_1101-2021_01_08_425864 412 13 acid acid NNP 10_1101-2021_01_08_425864 412 14 consumption consumption NN 10_1101-2021_01_08_425864 412 15 , , , 10_1101-2021_01_08_425864 412 16 which which WDT 10_1101-2021_01_08_425864 412 17 in in IN 10_1101-2021_01_08_425864 412 18 turn turn NN 10_1101-2021_01_08_425864 412 19 diminished diminish VBD 10_1101-2021_01_08_425864 412 20 the the DT 10_1101-2021_01_08_425864 412 21 oxidative oxidative JJ 10_1101-2021_01_08_425864 412 22 phosphorylation phosphorylation NN 10_1101-2021_01_08_425864 412 23 635 635 CD 10_1101-2021_01_08_425864 412 24 activity activity NN 10_1101-2021_01_08_425864 412 25 , , , 10_1101-2021_01_08_425864 412 26 internal internal JJ 10_1101-2021_01_08_425864 412 27 ATP atp NN 10_1101-2021_01_08_425864 412 28 levels level NNS 10_1101-2021_01_08_425864 412 29 and and CC 10_1101-2021_01_08_425864 412 30 the the DT 10_1101-2021_01_08_425864 412 31 total total JJ 10_1101-2021_01_08_425864 412 32 reductive reductive JJ 10_1101-2021_01_08_425864 412 33 activity activity NN 10_1101-2021_01_08_425864 412 34 of of IN 10_1101-2021_01_08_425864 412 35 parasites parasite NNS 10_1101-2021_01_08_425864 412 36 under under IN 10_1101-2021_01_08_425864 412 37 severe severe JJ 10_1101-2021_01_08_425864 412 38 nutritional nutritional JJ 10_1101-2021_01_08_425864 412 39 stress stress NN 10_1101-2021_01_08_425864 412 40 . . . 10_1101-2021_01_08_425864 413 1 636 636 CD 10_1101-2021_01_08_425864 413 2 Even even RB 10_1101-2021_01_08_425864 413 3 more more RBR 10_1101-2021_01_08_425864 413 4 notably notably RB 10_1101-2021_01_08_425864 413 5 , , , 10_1101-2021_01_08_425864 413 6 we -PRON- PRP 10_1101-2021_01_08_425864 413 7 showed show VBD 10_1101-2021_01_08_425864 413 8 that that IN 10_1101-2021_01_08_425864 413 9 under under IN 10_1101-2021_01_08_425864 413 10 these these DT 10_1101-2021_01_08_425864 413 11 conditions condition NNS 10_1101-2021_01_08_425864 413 12 , , , 10_1101-2021_01_08_425864 413 13 the the DT 10_1101-2021_01_08_425864 413 14 lipids lipid NNS 10_1101-2021_01_08_425864 413 15 stored store VBN 10_1101-2021_01_08_425864 413 16 in in IN 10_1101-2021_01_08_425864 413 17 lipid lipid NN 10_1101-2021_01_08_425864 413 18 droplets droplet NNS 10_1101-2021_01_08_425864 413 19 [ [ -LRB- 10_1101-2021_01_08_425864 413 20 51,52 51,52 CD 10_1101-2021_01_08_425864 413 21 ] ] -RRB- 10_1101-2021_01_08_425864 413 22 637 637 CD 10_1101-2021_01_08_425864 413 23 are be VBP 10_1101-2021_01_08_425864 413 24 consumed consume VBN 10_1101-2021_01_08_425864 413 25 . . . 10_1101-2021_01_08_425864 414 1 Unlike unlike IN 10_1101-2021_01_08_425864 414 2 what what WP 10_1101-2021_01_08_425864 414 3 has have VBZ 10_1101-2021_01_08_425864 414 4 been be VBN 10_1101-2021_01_08_425864 414 5 observed observe VBN 10_1101-2021_01_08_425864 414 6 in in IN 10_1101-2021_01_08_425864 414 7 procyclic procyclic JJ 10_1101-2021_01_08_425864 414 8 forms form NNS 10_1101-2021_01_08_425864 414 9 of of IN 10_1101-2021_01_08_425864 414 10 T. t. NN 10_1101-2021_01_08_425864 414 11 brucei brucei NN 10_1101-2021_01_08_425864 414 12 , , , 10_1101-2021_01_08_425864 414 13 in in IN 10_1101-2021_01_08_425864 414 14 which which WDT 10_1101-2021_01_08_425864 414 15 the the DT 10_1101-2021_01_08_425864 414 16 function function NN 10_1101-2021_01_08_425864 414 17 638 638 CD 10_1101-2021_01_08_425864 414 18 of of IN 10_1101-2021_01_08_425864 414 19 lipid lipid NN 10_1101-2021_01_08_425864 414 20 droplets droplet NNS 10_1101-2021_01_08_425864 414 21 is be VBZ 10_1101-2021_01_08_425864 414 22 not not RB 10_1101-2021_01_08_425864 414 23 clear clear JJ 10_1101-2021_01_08_425864 414 24 [ [ -LRB- 10_1101-2021_01_08_425864 414 25 53 53 CD 10_1101-2021_01_08_425864 414 26 ] ] -RRB- 10_1101-2021_01_08_425864 414 27 , , , 10_1101-2021_01_08_425864 414 28 our -PRON- PRP$ 10_1101-2021_01_08_425864 414 29 results result NNS 10_1101-2021_01_08_425864 414 30 show show VBP 10_1101-2021_01_08_425864 414 31 that that IN 10_1101-2021_01_08_425864 414 32 in in IN 10_1101-2021_01_08_425864 414 33 T. t. NN 10_1101-2021_01_08_425864 414 34 cruzi cruzi NNS 10_1101-2021_01_08_425864 414 35 , , , 10_1101-2021_01_08_425864 414 36 they -PRON- PRP 10_1101-2021_01_08_425864 414 37 are be VBP 10_1101-2021_01_08_425864 414 38 committed commit VBN 10_1101-2021_01_08_425864 414 39 to to TO 10_1101-2021_01_08_425864 414 40 epimastigote epimastigote VB 10_1101-2021_01_08_425864 414 41 639 639 CD 10_1101-2021_01_08_425864 414 42 survival survival NN 10_1101-2021_01_08_425864 414 43 under under IN 10_1101-2021_01_08_425864 414 44 extreme extreme JJ 10_1101-2021_01_08_425864 414 45 metabolic metabolic JJ 10_1101-2021_01_08_425864 414 46 stress stress NN 10_1101-2021_01_08_425864 414 47 . . . 10_1101-2021_01_08_425864 415 1 Of of RB 10_1101-2021_01_08_425864 415 2 course course RB 10_1101-2021_01_08_425864 415 3 , , , 10_1101-2021_01_08_425864 415 4 the the DT 10_1101-2021_01_08_425864 415 5 contribution contribution NN 10_1101-2021_01_08_425864 415 6 of of IN 10_1101-2021_01_08_425864 415 7 other other JJ 10_1101-2021_01_08_425864 415 8 metabolic metabolic JJ 10_1101-2021_01_08_425864 415 9 sources source NNS 10_1101-2021_01_08_425864 415 10 and and CC 10_1101-2021_01_08_425864 415 11 640 640 CD 10_1101-2021_01_08_425864 415 12 processes process NNS 10_1101-2021_01_08_425864 415 13 such such JJ 10_1101-2021_01_08_425864 415 14 as as IN 10_1101-2021_01_08_425864 415 15 autophagy autophagy NNP 10_1101-2021_01_08_425864 415 16 in in IN 10_1101-2021_01_08_425864 415 17 coping cope VBG 10_1101-2021_01_08_425864 415 18 with with IN 10_1101-2021_01_08_425864 415 19 nutritional nutritional JJ 10_1101-2021_01_08_425864 415 20 stress stress NN 10_1101-2021_01_08_425864 415 21 can can MD 10_1101-2021_01_08_425864 415 22 not not RB 10_1101-2021_01_08_425864 415 23 be be VB 10_1101-2021_01_08_425864 415 24 ruled rule VBN 10_1101-2021_01_08_425864 415 25 out out RP 10_1101-2021_01_08_425864 415 26 [ [ -LRB- 10_1101-2021_01_08_425864 415 27 54 54 CD 10_1101-2021_01_08_425864 415 28 ] ] -RRB- 10_1101-2021_01_08_425864 415 29 . . . 10_1101-2021_01_08_425864 416 1 641 641 CD 10_1101-2021_01_08_425864 416 2 Multiple multiple JJ 10_1101-2021_01_08_425864 416 3 metabolic metabolic NN 10_1101-2021_01_08_425864 416 4 factors factor NNS 10_1101-2021_01_08_425864 416 5 has have VBZ 10_1101-2021_01_08_425864 416 6 been be VBN 10_1101-2021_01_08_425864 416 7 involved involve VBN 10_1101-2021_01_08_425864 416 8 in in IN 10_1101-2021_01_08_425864 416 9 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 416 10 , , , 10_1101-2021_01_08_425864 416 11 such such JJ 10_1101-2021_01_08_425864 416 12 as as IN 10_1101-2021_01_08_425864 416 13 the the DT 10_1101-2021_01_08_425864 416 14 proline proline NN 10_1101-2021_01_08_425864 416 15 , , , 10_1101-2021_01_08_425864 416 16 aspartate aspartate NN 10_1101-2021_01_08_425864 416 17 , , , 10_1101-2021_01_08_425864 416 18 642 642 CD 10_1101-2021_01_08_425864 416 19 glutamate glutamate NN 10_1101-2021_01_08_425864 416 20 [ [ -LRB- 10_1101-2021_01_08_425864 416 21 55 55 CD 10_1101-2021_01_08_425864 416 22 ] ] -RRB- 10_1101-2021_01_08_425864 416 23 , , , 10_1101-2021_01_08_425864 416 24 glutamine glutamine NN 10_1101-2021_01_08_425864 416 25 [ [ -LRB- 10_1101-2021_01_08_425864 416 26 17 17 CD 10_1101-2021_01_08_425864 416 27 ] ] -RRB- 10_1101-2021_01_08_425864 416 28 and and CC 10_1101-2021_01_08_425864 416 29 lipids lipid NNS 10_1101-2021_01_08_425864 416 30 present present JJ 10_1101-2021_01_08_425864 416 31 in in IN 10_1101-2021_01_08_425864 416 32 the the DT 10_1101-2021_01_08_425864 416 33 triatomine triatomine JJ 10_1101-2021_01_08_425864 416 34 digestive digestive JJ 10_1101-2021_01_08_425864 416 35 tract tract NN 10_1101-2021_01_08_425864 416 36 [ [ -LRB- 10_1101-2021_01_08_425864 416 37 56 56 CD 10_1101-2021_01_08_425864 416 38 ] ] -RRB- 10_1101-2021_01_08_425864 416 39 . . . 10_1101-2021_01_08_425864 417 1 Interestingly interestingly RB 10_1101-2021_01_08_425864 417 2 , , , 10_1101-2021_01_08_425864 417 3 643 643 CD 10_1101-2021_01_08_425864 417 4 the the DT 10_1101-2021_01_08_425864 417 5 occurrence occurrence NN 10_1101-2021_01_08_425864 417 6 of of IN 10_1101-2021_01_08_425864 417 7 metacyclic metacyclic JJ 10_1101-2021_01_08_425864 417 8 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 417 9 in in IN 10_1101-2021_01_08_425864 417 10 culture culture NN 10_1101-2021_01_08_425864 417 11 leads lead VBZ 10_1101-2021_01_08_425864 417 12 to to IN 10_1101-2021_01_08_425864 417 13 an an DT 10_1101-2021_01_08_425864 417 14 increase increase NN 10_1101-2021_01_08_425864 417 15 in in IN 10_1101-2021_01_08_425864 417 16 CO2 CO2 NNP 10_1101-2021_01_08_425864 417 17 production production NN 10_1101-2021_01_08_425864 417 18 from from IN 10_1101-2021_01_08_425864 417 19 644 644 CD 10_1101-2021_01_08_425864 417 20 labelled label VBN 10_1101-2021_01_08_425864 417 21 palmitate palmitate NN 10_1101-2021_01_08_425864 417 22 [ [ -LRB- 10_1101-2021_01_08_425864 417 23 39 39 CD 10_1101-2021_01_08_425864 417 24 ] ] -RRB- 10_1101-2021_01_08_425864 417 25 . . . 10_1101-2021_01_08_425864 418 1 The the DT 10_1101-2021_01_08_425864 418 2 ETO ETO NNP 10_1101-2021_01_08_425864 418 3 treatment treatment NN 10_1101-2021_01_08_425864 418 4 inhibited inhibit VBD 10_1101-2021_01_08_425864 418 5 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 418 6 in in IN 10_1101-2021_01_08_425864 418 7 vitro vitro FW 10_1101-2021_01_08_425864 418 8 , , , 10_1101-2021_01_08_425864 418 9 showing show VBG 10_1101-2021_01_08_425864 418 10 that that IN 10_1101-2021_01_08_425864 418 11 the the DT 10_1101-2021_01_08_425864 418 12 645 645 CD 10_1101-2021_01_08_425864 418 13 consumption consumption NN 10_1101-2021_01_08_425864 418 14 of of IN 10_1101-2021_01_08_425864 418 15 internal internal JJ 10_1101-2021_01_08_425864 418 16 fatty fatty NN 10_1101-2021_01_08_425864 418 17 acids acid NNS 10_1101-2021_01_08_425864 418 18 is be VBZ 10_1101-2021_01_08_425864 418 19 important important JJ 10_1101-2021_01_08_425864 418 20 for for IN 10_1101-2021_01_08_425864 418 21 cell cell NN 10_1101-2021_01_08_425864 418 22 differentiation differentiation NN 10_1101-2021_01_08_425864 418 23 . . . 10_1101-2021_01_08_425864 419 1 Consequently consequently RB 10_1101-2021_01_08_425864 419 2 , , , 10_1101-2021_01_08_425864 419 3 we -PRON- PRP 10_1101-2021_01_08_425864 419 4 propose propose VBP 10_1101-2021_01_08_425864 419 5 646 646 CD 10_1101-2021_01_08_425864 419 6 that that IN 10_1101-2021_01_08_425864 419 7 lipids lipid NNS 10_1101-2021_01_08_425864 419 8 are be VBP 10_1101-2021_01_08_425864 419 9 not not RB 10_1101-2021_01_08_425864 419 10 only only RB 10_1101-2021_01_08_425864 419 11 external external JJ 10_1101-2021_01_08_425864 419 12 signals signal NNS 10_1101-2021_01_08_425864 419 13 of of IN 10_1101-2021_01_08_425864 419 14 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 419 15 , , , 10_1101-2021_01_08_425864 419 16 as as IN 10_1101-2021_01_08_425864 419 17 previously previously RB 10_1101-2021_01_08_425864 419 18 suggested suggest VBN 10_1101-2021_01_08_425864 419 19 [ [ -LRB- 10_1101-2021_01_08_425864 419 20 56 56 CD 10_1101-2021_01_08_425864 419 21 ] ] -RRB- 10_1101-2021_01_08_425864 419 22 , , , 10_1101-2021_01_08_425864 419 23 but but CC 10_1101-2021_01_08_425864 419 24 they -PRON- PRP 10_1101-2021_01_08_425864 419 25 647 647 CD 10_1101-2021_01_08_425864 419 26 also also RB 10_1101-2021_01_08_425864 419 27 have have VBP 10_1101-2021_01_08_425864 419 28 a a DT 10_1101-2021_01_08_425864 419 29 central central JJ 10_1101-2021_01_08_425864 419 30 role role NN 10_1101-2021_01_08_425864 419 31 in in IN 10_1101-2021_01_08_425864 419 32 the the DT 10_1101-2021_01_08_425864 419 33 bioenergetics bioenergetic NNS 10_1101-2021_01_08_425864 419 34 of of IN 10_1101-2021_01_08_425864 419 35 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 419 36 . . . 10_1101-2021_01_08_425864 420 1 As as IN 10_1101-2021_01_08_425864 420 2 in in IN 10_1101-2021_01_08_425864 420 3 the the DT 10_1101-2021_01_08_425864 420 4 oxidation oxidation NN 10_1101-2021_01_08_425864 420 5 of of IN 10_1101-2021_01_08_425864 420 6 several several JJ 10_1101-2021_01_08_425864 420 7 amino amino JJ 10_1101-2021_01_08_425864 420 8 648 648 CD 10_1101-2021_01_08_425864 420 9 acids acid NNS 10_1101-2021_01_08_425864 420 10 , , , 10_1101-2021_01_08_425864 420 11 the the DT 10_1101-2021_01_08_425864 420 12 acetyl acetyl NNP 10_1101-2021_01_08_425864 420 13 - - HYPH 10_1101-2021_01_08_425864 420 14 CoA CoA NNP 10_1101-2021_01_08_425864 420 15 produced produce VBN 10_1101-2021_01_08_425864 420 16 from from IN 10_1101-2021_01_08_425864 420 17 beta beta NN 10_1101-2021_01_08_425864 420 18 - - HYPH 10_1101-2021_01_08_425864 420 19 oxidation oxidation NN 10_1101-2021_01_08_425864 420 20 and and CC 10_1101-2021_01_08_425864 420 21 probably probably RB 10_1101-2021_01_08_425864 420 22 the the DT 10_1101-2021_01_08_425864 420 23 reduced reduce VBN 10_1101-2021_01_08_425864 420 24 cofactors cofactor NNS 10_1101-2021_01_08_425864 420 25 resulting result VBG 10_1101-2021_01_08_425864 420 26 649 649 CD 10_1101-2021_01_08_425864 420 27 from from IN 10_1101-2021_01_08_425864 420 28 these these DT 10_1101-2021_01_08_425864 420 29 processes process NNS 10_1101-2021_01_08_425864 420 30 are be VBP 10_1101-2021_01_08_425864 420 31 contributing contribute VBG 10_1101-2021_01_08_425864 420 32 to to IN 10_1101-2021_01_08_425864 420 33 the the DT 10_1101-2021_01_08_425864 420 34 mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 420 35 ATP atp NN 10_1101-2021_01_08_425864 420 36 production production NN 10_1101-2021_01_08_425864 420 37 necessary necessary JJ 10_1101-2021_01_08_425864 420 38 to to TO 10_1101-2021_01_08_425864 420 39 support support VB 10_1101-2021_01_08_425864 420 40 this this DT 10_1101-2021_01_08_425864 420 41 650 650 CD 10_1101-2021_01_08_425864 420 42 differentiation differentiation NN 10_1101-2021_01_08_425864 420 43 step step NN 10_1101-2021_01_08_425864 420 44 . . . 10_1101-2021_01_08_425864 421 1 651 651 CD 10_1101-2021_01_08_425864 421 2 In in IN 10_1101-2021_01_08_425864 421 3 conclusion conclusion NN 10_1101-2021_01_08_425864 421 4 , , , 10_1101-2021_01_08_425864 421 5 fatty fatty NN 10_1101-2021_01_08_425864 421 6 acids acid NNS 10_1101-2021_01_08_425864 421 7 are be VBP 10_1101-2021_01_08_425864 421 8 important important JJ 10_1101-2021_01_08_425864 421 9 carbon carbon NN 10_1101-2021_01_08_425864 421 10 sources source NNS 10_1101-2021_01_08_425864 421 11 for for IN 10_1101-2021_01_08_425864 421 12 T. T. NNP 10_1101-2021_01_08_425864 421 13 cruzi cruzi NNS 10_1101-2021_01_08_425864 421 14 epimastigotes epimastigote VBZ 10_1101-2021_01_08_425864 421 15 in in IN 10_1101-2021_01_08_425864 421 16 the the DT 10_1101-2021_01_08_425864 421 17 652 652 CD 10_1101-2021_01_08_425864 421 18 absence absence NN 10_1101-2021_01_08_425864 421 19 of of IN 10_1101-2021_01_08_425864 421 20 glucose glucose NN 10_1101-2021_01_08_425864 421 21 . . . 10_1101-2021_01_08_425864 422 1 Palmitate palmitate NN 10_1101-2021_01_08_425864 422 2 can can MD 10_1101-2021_01_08_425864 422 3 be be VB 10_1101-2021_01_08_425864 422 4 taken take VBN 10_1101-2021_01_08_425864 422 5 up up RP 10_1101-2021_01_08_425864 422 6 by by IN 10_1101-2021_01_08_425864 422 7 the the DT 10_1101-2021_01_08_425864 422 8 cells cell NNS 10_1101-2021_01_08_425864 422 9 and and CC 10_1101-2021_01_08_425864 422 10 fuel fuel VB 10_1101-2021_01_08_425864 422 11 the the DT 10_1101-2021_01_08_425864 422 12 TCA TCA NNP 10_1101-2021_01_08_425864 422 13 cycle cycle NN 10_1101-2021_01_08_425864 422 14 by by IN 10_1101-2021_01_08_425864 422 15 producing produce VBG 10_1101-2021_01_08_425864 422 16 653 653 CD 10_1101-2021_01_08_425864 422 17 acetyl acetyl NN 10_1101-2021_01_08_425864 422 18 - - HYPH 10_1101-2021_01_08_425864 422 19 CoA CoA NNP 10_1101-2021_01_08_425864 422 20 , , , 10_1101-2021_01_08_425864 422 21 the the DT 10_1101-2021_01_08_425864 422 22 oxidation oxidation NN 10_1101-2021_01_08_425864 422 23 of of IN 10_1101-2021_01_08_425864 422 24 which which WDT 10_1101-2021_01_08_425864 422 25 generates generate VBZ 10_1101-2021_01_08_425864 422 26 CO2 CO2 NNP 10_1101-2021_01_08_425864 422 27 . . . 10_1101-2021_01_08_425864 423 1 However however RB 10_1101-2021_01_08_425864 423 2 , , , 10_1101-2021_01_08_425864 423 3 in in IN 10_1101-2021_01_08_425864 423 4 the the DT 10_1101-2021_01_08_425864 423 5 absence absence NN 10_1101-2021_01_08_425864 423 6 of of IN 10_1101-2021_01_08_425864 423 7 external external JJ 10_1101-2021_01_08_425864 423 8 carbon carbon NN 10_1101-2021_01_08_425864 423 9 .CC .CC . 10_1101-2021_01_08_425864 423 10 - - : 10_1101-2021_01_08_425864 423 11 BY by IN 10_1101-2021_01_08_425864 423 12 4.0 4.0 CD 10_1101-2021_01_08_425864 423 13 International international JJ 10_1101-2021_01_08_425864 423 14 licensemade licensemade NN 10_1101-2021_01_08_425864 423 15 available available JJ 10_1101-2021_01_08_425864 423 16 under under IN 10_1101-2021_01_08_425864 423 17 a a DT 10_1101-2021_01_08_425864 423 18 ( ( -LRB- 10_1101-2021_01_08_425864 423 19 which which WDT 10_1101-2021_01_08_425864 423 20 was be VBD 10_1101-2021_01_08_425864 423 21 not not RB 10_1101-2021_01_08_425864 423 22 certified certify VBN 10_1101-2021_01_08_425864 423 23 by by IN 10_1101-2021_01_08_425864 423 24 peer peer NN 10_1101-2021_01_08_425864 423 25 review review NN 10_1101-2021_01_08_425864 423 26 ) ) -RRB- 10_1101-2021_01_08_425864 423 27 is be VBZ 10_1101-2021_01_08_425864 423 28 the the DT 10_1101-2021_01_08_425864 423 29 author author NN 10_1101-2021_01_08_425864 423 30 / / SYM 10_1101-2021_01_08_425864 423 31 funder funder NN 10_1101-2021_01_08_425864 423 32 , , , 10_1101-2021_01_08_425864 423 33 who who WP 10_1101-2021_01_08_425864 423 34 has have VBZ 10_1101-2021_01_08_425864 423 35 granted grant VBN 10_1101-2021_01_08_425864 423 36 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 423 37 a a DT 10_1101-2021_01_08_425864 423 38 license license NN 10_1101-2021_01_08_425864 423 39 to to TO 10_1101-2021_01_08_425864 423 40 display display VB 10_1101-2021_01_08_425864 423 41 the the DT 10_1101-2021_01_08_425864 423 42 preprint preprint NN 10_1101-2021_01_08_425864 423 43 in in IN 10_1101-2021_01_08_425864 423 44 perpetuity perpetuity NN 10_1101-2021_01_08_425864 423 45 . . . 10_1101-2021_01_08_425864 424 1 It -PRON- PRP 10_1101-2021_01_08_425864 424 2 is be VBZ 10_1101-2021_01_08_425864 424 3 The the DT 10_1101-2021_01_08_425864 424 4 copyright copyright NN 10_1101-2021_01_08_425864 424 5 holder holder NN 10_1101-2021_01_08_425864 424 6 for for IN 10_1101-2021_01_08_425864 424 7 this this DT 10_1101-2021_01_08_425864 424 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 424 9 version version NN 10_1101-2021_01_08_425864 424 10 posted post VBD 10_1101-2021_01_08_425864 424 11 January January NNP 10_1101-2021_01_08_425864 424 12 8 8 CD 10_1101-2021_01_08_425864 424 13 , , , 10_1101-2021_01_08_425864 424 14 2021 2021 CD 10_1101-2021_01_08_425864 424 15 . . . 10_1101-2021_01_08_425864 424 16 ; ; : 10_1101-2021_01_08_425864 424 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 424 18 : : : 10_1101-2021_01_08_425864 424 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 424 20 preprint preprint NN 10_1101-2021_01_08_425864 424 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 424 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 424 23 654 654 CD 10_1101-2021_01_08_425864 424 24 sources source NNS 10_1101-2021_01_08_425864 424 25 , , , 10_1101-2021_01_08_425864 424 26 lipid lipid NN 10_1101-2021_01_08_425864 424 27 droplets droplet NNS 10_1101-2021_01_08_425864 424 28 become become VBP 10_1101-2021_01_08_425864 424 29 the the DT 10_1101-2021_01_08_425864 424 30 primary primary JJ 10_1101-2021_01_08_425864 424 31 sources source NNS 10_1101-2021_01_08_425864 424 32 of of IN 10_1101-2021_01_08_425864 424 33 fatty fatty NN 10_1101-2021_01_08_425864 424 34 acids acid NNS 10_1101-2021_01_08_425864 424 35 , , , 10_1101-2021_01_08_425864 424 36 helping help VBG 10_1101-2021_01_08_425864 424 37 the the DT 10_1101-2021_01_08_425864 424 38 organism organism NN 10_1101-2021_01_08_425864 424 39 to to TO 10_1101-2021_01_08_425864 424 40 survive survive VB 10_1101-2021_01_08_425864 424 41 655 655 CD 10_1101-2021_01_08_425864 424 42 nutritional nutritional JJ 10_1101-2021_01_08_425864 424 43 stress stress NN 10_1101-2021_01_08_425864 424 44 . . . 10_1101-2021_01_08_425864 425 1 Importantly importantly RB 10_1101-2021_01_08_425864 425 2 , , , 10_1101-2021_01_08_425864 425 3 FAO FAO NNP 10_1101-2021_01_08_425864 425 4 supports support VBZ 10_1101-2021_01_08_425864 425 5 endogenous endogenous JJ 10_1101-2021_01_08_425864 425 6 respiration respiration NN 10_1101-2021_01_08_425864 425 7 rates rate NNS 10_1101-2021_01_08_425864 425 8 and and CC 10_1101-2021_01_08_425864 425 9 ATP atp NN 10_1101-2021_01_08_425864 425 10 production production NN 10_1101-2021_01_08_425864 425 11 and and CC 10_1101-2021_01_08_425864 425 12 656 656 CD 10_1101-2021_01_08_425864 425 13 powers power NNS 10_1101-2021_01_08_425864 425 14 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 425 15 . . . 10_1101-2021_01_08_425864 426 1 657 657 CD 10_1101-2021_01_08_425864 426 2 658 658 CD 10_1101-2021_01_08_425864 426 3 659 659 CD 10_1101-2021_01_08_425864 426 4 Acknowledgements acknowledgement NNS 10_1101-2021_01_08_425864 426 5 660 660 CD 10_1101-2021_01_08_425864 426 6 We -PRON- PRP 10_1101-2021_01_08_425864 426 7 thank thank VBP 10_1101-2021_01_08_425864 426 8 the the DT 10_1101-2021_01_08_425864 426 9 Core Core NNP 10_1101-2021_01_08_425864 426 10 Facility Facility NNP 10_1101-2021_01_08_425864 426 11 for for IN 10_1101-2021_01_08_425864 426 12 Scientific Scientific NNP 10_1101-2021_01_08_425864 426 13 Research Research NNP 10_1101-2021_01_08_425864 426 14 at at IN 10_1101-2021_01_08_425864 426 15 the the DT 10_1101-2021_01_08_425864 426 16 University University NNP 10_1101-2021_01_08_425864 426 17 of of IN 10_1101-2021_01_08_425864 426 18 Sao Sao NNP 10_1101-2021_01_08_425864 426 19 Paulo Paulo NNP 10_1101-2021_01_08_425864 426 20 ( ( -LRB- 10_1101-2021_01_08_425864 426 21 CEFAP- CEFAP- NNP 10_1101-2021_01_08_425864 426 22 661 661 CD 10_1101-2021_01_08_425864 426 23 USP USP NNP 10_1101-2021_01_08_425864 426 24 / / SYM 10_1101-2021_01_08_425864 426 25 FLUIR FLUIR NNP 10_1101-2021_01_08_425864 426 26 ) ) -RRB- 10_1101-2021_01_08_425864 426 27 for for IN 10_1101-2021_01_08_425864 426 28 the the DT 10_1101-2021_01_08_425864 426 29 flow flow NN 10_1101-2021_01_08_425864 426 30 cytometry cytometry NN 10_1101-2021_01_08_425864 426 31 analysis analysis NN 10_1101-2021_01_08_425864 426 32 and and CC 10_1101-2021_01_08_425864 426 33 Dr. Dr. NNP 10_1101-2021_01_08_425864 426 34 Mauro Mauro NNP 10_1101-2021_01_08_425864 426 35 Javier Javier NNP 10_1101-2021_01_08_425864 426 36 Veliz Veliz NNP 10_1101-2021_01_08_425864 426 37 Cortez Cortez NNP 10_1101-2021_01_08_425864 426 38 ( ( -LRB- 10_1101-2021_01_08_425864 426 39 Department Department NNP 10_1101-2021_01_08_425864 426 40 of of IN 10_1101-2021_01_08_425864 426 41 662 662 CD 10_1101-2021_01_08_425864 426 42 Parasitology Parasitology NNP 10_1101-2021_01_08_425864 426 43 , , , 10_1101-2021_01_08_425864 426 44 ICB ICB NNP 10_1101-2021_01_08_425864 426 45 - - HYPH 10_1101-2021_01_08_425864 426 46 USP USP NNP 10_1101-2021_01_08_425864 426 47 ) ) -RRB- 10_1101-2021_01_08_425864 426 48 for for IN 10_1101-2021_01_08_425864 426 49 the the DT 10_1101-2021_01_08_425864 426 50 microscopy microscopy JJ 10_1101-2021_01_08_425864 426 51 work work NN 10_1101-2021_01_08_425864 426 52 . . . 10_1101-2021_01_08_425864 427 1 We -PRON- PRP 10_1101-2021_01_08_425864 427 2 thank thank VBP 10_1101-2021_01_08_425864 427 3 the the DT 10_1101-2021_01_08_425864 427 4 Core Core NNP 10_1101-2021_01_08_425864 427 5 Facility Facility NNP 10_1101-2021_01_08_425864 427 6 for for IN 10_1101-2021_01_08_425864 427 7 Scientific Scientific NNP 10_1101-2021_01_08_425864 427 8 Research Research NNP 10_1101-2021_01_08_425864 427 9 663 663 CD 10_1101-2021_01_08_425864 427 10 at at IN 10_1101-2021_01_08_425864 427 11 the the DT 10_1101-2021_01_08_425864 427 12 University University NNP 10_1101-2021_01_08_425864 427 13 of of IN 10_1101-2021_01_08_425864 427 14 Sao Sao NNP 10_1101-2021_01_08_425864 427 15 Paulo Paulo NNP 10_1101-2021_01_08_425864 427 16 ( ( -LRB- 10_1101-2021_01_08_425864 427 17 CEFAP CEFAP NNP 10_1101-2021_01_08_425864 427 18 - - HYPH 10_1101-2021_01_08_425864 427 19 USP USP NNP 10_1101-2021_01_08_425864 427 20 / / SYM 10_1101-2021_01_08_425864 427 21 FLUIR FLUIR NNP 10_1101-2021_01_08_425864 427 22 ) ) -RRB- 10_1101-2021_01_08_425864 427 23 for for IN 10_1101-2021_01_08_425864 427 24 the the DT 10_1101-2021_01_08_425864 427 25 flow flow NN 10_1101-2021_01_08_425864 427 26 cytometry cytometry NN 10_1101-2021_01_08_425864 427 27 analysis analysis NN 10_1101-2021_01_08_425864 427 28 and and CC 10_1101-2021_01_08_425864 427 29 Dr. Dr. NNP 10_1101-2021_01_08_425864 427 30 Mauro Mauro NNP 10_1101-2021_01_08_425864 427 31 664 664 CD 10_1101-2021_01_08_425864 427 32 Javier Javier NNP 10_1101-2021_01_08_425864 427 33 Veliz Veliz NNP 10_1101-2021_01_08_425864 427 34 Cortez Cortez NNP 10_1101-2021_01_08_425864 427 35 ( ( -LRB- 10_1101-2021_01_08_425864 427 36 Department Department NNP 10_1101-2021_01_08_425864 427 37 of of IN 10_1101-2021_01_08_425864 427 38 Parasitology Parasitology NNP 10_1101-2021_01_08_425864 427 39 , , , 10_1101-2021_01_08_425864 427 40 ICB ICB NNP 10_1101-2021_01_08_425864 427 41 - - HYPH 10_1101-2021_01_08_425864 427 42 USP USP NNP 10_1101-2021_01_08_425864 427 43 ) ) -RRB- 10_1101-2021_01_08_425864 427 44 for for IN 10_1101-2021_01_08_425864 427 45 the the DT 10_1101-2021_01_08_425864 427 46 microscopy microscopy JJ 10_1101-2021_01_08_425864 427 47 support support NN 10_1101-2021_01_08_425864 427 48 . . . 10_1101-2021_01_08_425864 428 1 665 665 CD 10_1101-2021_01_08_425864 428 2 666 666 CD 10_1101-2021_01_08_425864 428 3 References reference NNS 10_1101-2021_01_08_425864 428 4 667 667 CD 10_1101-2021_01_08_425864 428 5 [ [ -LRB- 10_1101-2021_01_08_425864 428 6 1 1 CD 10_1101-2021_01_08_425864 428 7 ] ] -RRB- 10_1101-2021_01_08_425864 428 8 WHO who WP 10_1101-2021_01_08_425864 428 9 | | NNP 10_1101-2021_01_08_425864 428 10 Chagas Chagas NNP 10_1101-2021_01_08_425864 428 11 disease disease NN 10_1101-2021_01_08_425864 428 12 ( ( -LRB- 10_1101-2021_01_08_425864 428 13 American american JJ 10_1101-2021_01_08_425864 428 14 trypanosomiasis trypanosomiasis NN 10_1101-2021_01_08_425864 428 15 ) ) -RRB- 10_1101-2021_01_08_425864 428 16 , , , 10_1101-2021_01_08_425864 428 17 WHO who WP 10_1101-2021_01_08_425864 428 18 . . . 10_1101-2021_01_08_425864 429 1 ( ( -LRB- 10_1101-2021_01_08_425864 429 2 2018 2018 CD 10_1101-2021_01_08_425864 429 3 ) ) -RRB- 10_1101-2021_01_08_425864 429 4 . . . 10_1101-2021_01_08_425864 430 1 668 668 CD 10_1101-2021_01_08_425864 430 2 https://www.who.int/chagas/en/ https://www.who.int/chagas/en/ NNP 10_1101-2021_01_08_425864 430 3 ( ( -LRB- 10_1101-2021_01_08_425864 430 4 accessed access VBN 10_1101-2021_01_08_425864 430 5 January January NNP 10_1101-2021_01_08_425864 430 6 29 29 CD 10_1101-2021_01_08_425864 430 7 , , , 10_1101-2021_01_08_425864 430 8 2019 2019 CD 10_1101-2021_01_08_425864 430 9 ) ) -RRB- 10_1101-2021_01_08_425864 430 10 . . . 10_1101-2021_01_08_425864 431 1 669 669 CD 10_1101-2021_01_08_425864 431 2 [ [ -LRB- 10_1101-2021_01_08_425864 431 3 2 2 CD 10_1101-2021_01_08_425864 431 4 ] ] -RRB- 10_1101-2021_01_08_425864 431 5 J.A. J.A. NNP 10_1101-2021_01_08_425864 432 1 Perez Perez NNP 10_1101-2021_01_08_425864 432 2 - - HYPH 10_1101-2021_01_08_425864 432 3 Molina Molina NNP 10_1101-2021_01_08_425864 432 4 , , , 10_1101-2021_01_08_425864 432 5 I. I. NNP 10_1101-2021_01_08_425864 432 6 Molina Molina NNP 10_1101-2021_01_08_425864 432 7 , , , 10_1101-2021_01_08_425864 432 8 Chagas Chagas NNP 10_1101-2021_01_08_425864 432 9 disease disease NN 10_1101-2021_01_08_425864 432 10 , , , 10_1101-2021_01_08_425864 432 11 Lancet Lancet NNP 10_1101-2021_01_08_425864 432 12 . . . 10_1101-2021_01_08_425864 433 1 391 391 CD 10_1101-2021_01_08_425864 433 2 ( ( -LRB- 10_1101-2021_01_08_425864 433 3 2018 2018 CD 10_1101-2021_01_08_425864 433 4 ) ) -RRB- 10_1101-2021_01_08_425864 433 5 82–94 82–94 CD 10_1101-2021_01_08_425864 433 6 . . . 10_1101-2021_01_08_425864 434 1 670 670 CD 10_1101-2021_01_08_425864 434 2 https://doi.org/10.1016/S0140-6736(17)31612-4 https://doi.org/10.1016/S0140-6736(17)31612-4 NNP 10_1101-2021_01_08_425864 434 3 . . . 10_1101-2021_01_08_425864 435 1 671 671 CD 10_1101-2021_01_08_425864 435 2 [ [ -LRB- 10_1101-2021_01_08_425864 435 3 3 3 CD 10_1101-2021_01_08_425864 435 4 ] ] -RRB- 10_1101-2021_01_08_425864 435 5 R. R. NNP 10_1101-2021_01_08_425864 435 6 de de NNP 10_1101-2021_01_08_425864 435 7 F.P. F.P. NNP 10_1101-2021_01_08_425864 436 1 Melo Melo NNP 10_1101-2021_01_08_425864 436 2 , , , 10_1101-2021_01_08_425864 436 3 A.A. A.A. NNP 10_1101-2021_01_08_425864 436 4 Guarneri Guarneri NNP 10_1101-2021_01_08_425864 436 5 , , , 10_1101-2021_01_08_425864 436 6 A.M. A.M. NNP 10_1101-2021_01_08_425864 436 7 Silber Silber NNP 10_1101-2021_01_08_425864 436 8 , , , 10_1101-2021_01_08_425864 436 9 The the DT 10_1101-2021_01_08_425864 436 10 influence influence NN 10_1101-2021_01_08_425864 436 11 of of IN 10_1101-2021_01_08_425864 436 12 environmental environmental JJ 10_1101-2021_01_08_425864 436 13 cues cue NNS 10_1101-2021_01_08_425864 436 14 on on IN 10_1101-2021_01_08_425864 436 15 the the DT 10_1101-2021_01_08_425864 436 16 672 672 CD 10_1101-2021_01_08_425864 436 17 development development NN 10_1101-2021_01_08_425864 436 18 of of IN 10_1101-2021_01_08_425864 436 19 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 436 20 cruzi cruzi NNS 10_1101-2021_01_08_425864 436 21 in in IN 10_1101-2021_01_08_425864 436 22 triatominae triatominae NN 10_1101-2021_01_08_425864 436 23 vector vector NN 10_1101-2021_01_08_425864 436 24 , , , 10_1101-2021_01_08_425864 436 25 Front Front NNP 10_1101-2021_01_08_425864 436 26 . . . 10_1101-2021_01_08_425864 437 1 Cell cell NN 10_1101-2021_01_08_425864 437 2 . . . 10_1101-2021_01_08_425864 438 1 Infect infect VB 10_1101-2021_01_08_425864 438 2 . . . 10_1101-2021_01_08_425864 439 1 Microbiol Microbiol NNP 10_1101-2021_01_08_425864 439 2 . . . 10_1101-2021_01_08_425864 440 1 10 10 CD 10_1101-2021_01_08_425864 440 2 673 673 CD 10_1101-2021_01_08_425864 440 3 ( ( -LRB- 10_1101-2021_01_08_425864 440 4 2020 2020 CD 10_1101-2021_01_08_425864 440 5 ) ) -RRB- 10_1101-2021_01_08_425864 440 6 27 27 CD 10_1101-2021_01_08_425864 440 7 . . . 10_1101-2021_01_08_425864 440 8 https://doi.org/10.3389/fcimb.2020.00027 https://doi.org/10.3389/fcimb.2020.00027 NNP 10_1101-2021_01_08_425864 440 9 . . . 10_1101-2021_01_08_425864 441 1 674 674 CD 10_1101-2021_01_08_425864 441 2 [ [ -LRB- 10_1101-2021_01_08_425864 441 3 4 4 CD 10_1101-2021_01_08_425864 441 4 ] ] -RRB- 10_1101-2021_01_08_425864 441 5 W. W. NNP 10_1101-2021_01_08_425864 441 6 De De NNP 10_1101-2021_01_08_425864 441 7 Souza Souza NNP 10_1101-2021_01_08_425864 441 8 , , , 10_1101-2021_01_08_425864 441 9 Basic basic JJ 10_1101-2021_01_08_425864 441 10 cell cell NN 10_1101-2021_01_08_425864 441 11 biology biology NN 10_1101-2021_01_08_425864 441 12 of of IN 10_1101-2021_01_08_425864 441 13 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 441 14 cruzi cruzi NNS 10_1101-2021_01_08_425864 441 15 . . . 10_1101-2021_01_08_425864 442 1 Curr curr UH 10_1101-2021_01_08_425864 442 2 . . . 10_1101-2021_01_08_425864 443 1 Pharm Pharm NNP 10_1101-2021_01_08_425864 443 2 . . . 10_1101-2021_01_08_425864 444 1 Des Des NNP 10_1101-2021_01_08_425864 444 2 . . . 10_1101-2021_01_08_425864 445 1 8 8 CD 10_1101-2021_01_08_425864 445 2 ( ( -LRB- 10_1101-2021_01_08_425864 445 3 2002 2002 CD 10_1101-2021_01_08_425864 445 4 ) ) -RRB- 10_1101-2021_01_08_425864 445 5 269–85 269–85 CD 10_1101-2021_01_08_425864 445 6 . . . 10_1101-2021_01_08_425864 446 1 675 675 CD 10_1101-2021_01_08_425864 446 2 http://www.ncbi.nlm.nih.gov/pubmed/11860366 http://www.ncbi.nlm.nih.gov/pubmed/11860366 NNS 10_1101-2021_01_08_425864 446 3 . . . 10_1101-2021_01_08_425864 447 1 676 676 CD 10_1101-2021_01_08_425864 447 2 [ [ -LRB- 10_1101-2021_01_08_425864 447 3 5 5 CD 10_1101-2021_01_08_425864 447 4 ] ] -RRB- 10_1101-2021_01_08_425864 447 5 P. P. NNP 10_1101-2021_01_08_425864 447 6 Lisvane Lisvane NNP 10_1101-2021_01_08_425864 447 7 Silva Silva NNP 10_1101-2021_01_08_425864 447 8 , , , 10_1101-2021_01_08_425864 447 9 B.S. B.S. NNP 10_1101-2021_01_08_425864 448 1 Mantilla Mantilla NNP 10_1101-2021_01_08_425864 448 2 , , , 10_1101-2021_01_08_425864 448 3 M.J. M.J. NNP 10_1101-2021_01_08_425864 448 4 Barison Barison NNP 10_1101-2021_01_08_425864 448 5 , , , 10_1101-2021_01_08_425864 448 6 C. C. NNP 10_1101-2021_01_08_425864 448 7 Wrenger Wrenger NNP 10_1101-2021_01_08_425864 448 8 , , , 10_1101-2021_01_08_425864 448 9 A.M. A.M. NNP 10_1101-2021_01_08_425864 448 10 Silber Silber NNP 10_1101-2021_01_08_425864 448 11 , , , 10_1101-2021_01_08_425864 448 12 The the DT 10_1101-2021_01_08_425864 448 13 uniqueness uniqueness NN 10_1101-2021_01_08_425864 448 14 of of IN 10_1101-2021_01_08_425864 448 15 677 677 CD 10_1101-2021_01_08_425864 448 16 the the DT 10_1101-2021_01_08_425864 448 17 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 448 18 cruzi cruzi NN 10_1101-2021_01_08_425864 448 19 mitochondrion mitochondrion NN 10_1101-2021_01_08_425864 448 20 : : : 10_1101-2021_01_08_425864 448 21 opportunities opportunity NNS 10_1101-2021_01_08_425864 448 22 to to TO 10_1101-2021_01_08_425864 448 23 identify identify VB 10_1101-2021_01_08_425864 448 24 new new JJ 10_1101-2021_01_08_425864 448 25 drug drug NN 10_1101-2021_01_08_425864 448 26 target target NN 10_1101-2021_01_08_425864 448 27 for for IN 10_1101-2021_01_08_425864 448 28 the the DT 10_1101-2021_01_08_425864 448 29 678 678 CD 10_1101-2021_01_08_425864 448 30 treatment treatment NN 10_1101-2021_01_08_425864 448 31 of of IN 10_1101-2021_01_08_425864 448 32 Chagas Chagas NNP 10_1101-2021_01_08_425864 448 33 disease disease NN 10_1101-2021_01_08_425864 448 34 , , , 10_1101-2021_01_08_425864 448 35 Curr Curr NNP 10_1101-2021_01_08_425864 448 36 Pharm Pharm NNP 10_1101-2021_01_08_425864 448 37 Des Des NNP 10_1101-2021_01_08_425864 448 38 . . . 10_1101-2021_01_08_425864 449 1 17 17 CD 10_1101-2021_01_08_425864 449 2 ( ( -LRB- 10_1101-2021_01_08_425864 449 3 2011 2011 CD 10_1101-2021_01_08_425864 449 4 ) ) -RRB- 10_1101-2021_01_08_425864 449 5 2074–2099 2074–2099 CD 10_1101-2021_01_08_425864 449 6 . . . 10_1101-2021_01_08_425864 450 1 679 679 CD 10_1101-2021_01_08_425864 450 2 https://www.ncbi.nlm.nih.gov/pubmed/21718252 https://www.ncbi.nlm.nih.gov/pubmed/21718252 NN 10_1101-2021_01_08_425864 450 3 . . . 10_1101-2021_01_08_425864 451 1 680 680 CD 10_1101-2021_01_08_425864 451 2 [ [ -LRB- 10_1101-2021_01_08_425864 451 3 6 6 CD 10_1101-2021_01_08_425864 451 4 ] ] -RRB- 10_1101-2021_01_08_425864 451 5 C. C. NNP 10_1101-2021_01_08_425864 451 6 Bern Bern NNP 10_1101-2021_01_08_425864 451 7 , , , 10_1101-2021_01_08_425864 451 8 Chagas Chagas NNP 10_1101-2021_01_08_425864 451 9 ’ ' '' 10_1101-2021_01_08_425864 451 10 Disease Disease NNP 10_1101-2021_01_08_425864 451 11 , , , 10_1101-2021_01_08_425864 451 12 N N NNP 10_1101-2021_01_08_425864 451 13 Engl Engl NNP 10_1101-2021_01_08_425864 451 14 J J NNP 10_1101-2021_01_08_425864 451 15 Med Med NNP 10_1101-2021_01_08_425864 451 16 . . . 10_1101-2021_01_08_425864 452 1 373 373 CD 10_1101-2021_01_08_425864 452 2 ( ( -LRB- 10_1101-2021_01_08_425864 452 3 2015 2015 CD 10_1101-2021_01_08_425864 452 4 ) ) -RRB- 10_1101-2021_01_08_425864 452 5 1882 1882 CD 10_1101-2021_01_08_425864 452 6 . . . 10_1101-2021_01_08_425864 453 1 681 681 CD 10_1101-2021_01_08_425864 453 2 https://doi.org/10.1056/NEJMc1510996 https://doi.org/10.1056/nejmc1510996 JJ 10_1101-2021_01_08_425864 453 3 .CC .CC , 10_1101-2021_01_08_425864 453 4 - - : 10_1101-2021_01_08_425864 453 5 BY by IN 10_1101-2021_01_08_425864 453 6 4.0 4.0 CD 10_1101-2021_01_08_425864 453 7 International international JJ 10_1101-2021_01_08_425864 453 8 licensemade licensemade NN 10_1101-2021_01_08_425864 453 9 available available JJ 10_1101-2021_01_08_425864 453 10 under under IN 10_1101-2021_01_08_425864 453 11 a a DT 10_1101-2021_01_08_425864 453 12 ( ( -LRB- 10_1101-2021_01_08_425864 453 13 which which WDT 10_1101-2021_01_08_425864 453 14 was be VBD 10_1101-2021_01_08_425864 453 15 not not RB 10_1101-2021_01_08_425864 453 16 certified certify VBN 10_1101-2021_01_08_425864 453 17 by by IN 10_1101-2021_01_08_425864 453 18 peer peer NN 10_1101-2021_01_08_425864 453 19 review review NN 10_1101-2021_01_08_425864 453 20 ) ) -RRB- 10_1101-2021_01_08_425864 453 21 is be VBZ 10_1101-2021_01_08_425864 453 22 the the DT 10_1101-2021_01_08_425864 453 23 author author NN 10_1101-2021_01_08_425864 453 24 / / SYM 10_1101-2021_01_08_425864 453 25 funder funder NN 10_1101-2021_01_08_425864 453 26 , , , 10_1101-2021_01_08_425864 453 27 who who WP 10_1101-2021_01_08_425864 453 28 has have VBZ 10_1101-2021_01_08_425864 453 29 granted grant VBN 10_1101-2021_01_08_425864 453 30 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 453 31 a a DT 10_1101-2021_01_08_425864 453 32 license license NN 10_1101-2021_01_08_425864 453 33 to to TO 10_1101-2021_01_08_425864 453 34 display display VB 10_1101-2021_01_08_425864 453 35 the the DT 10_1101-2021_01_08_425864 453 36 preprint preprint NN 10_1101-2021_01_08_425864 453 37 in in IN 10_1101-2021_01_08_425864 453 38 perpetuity perpetuity NN 10_1101-2021_01_08_425864 453 39 . . . 10_1101-2021_01_08_425864 454 1 It -PRON- PRP 10_1101-2021_01_08_425864 454 2 is be VBZ 10_1101-2021_01_08_425864 454 3 The the DT 10_1101-2021_01_08_425864 454 4 copyright copyright NN 10_1101-2021_01_08_425864 454 5 holder holder NN 10_1101-2021_01_08_425864 454 6 for for IN 10_1101-2021_01_08_425864 454 7 this this DT 10_1101-2021_01_08_425864 454 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 454 9 version version NN 10_1101-2021_01_08_425864 454 10 posted post VBD 10_1101-2021_01_08_425864 454 11 January January NNP 10_1101-2021_01_08_425864 454 12 8 8 CD 10_1101-2021_01_08_425864 454 13 , , , 10_1101-2021_01_08_425864 454 14 2021 2021 CD 10_1101-2021_01_08_425864 454 15 . . . 10_1101-2021_01_08_425864 454 16 ; ; : 10_1101-2021_01_08_425864 454 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 454 18 : : : 10_1101-2021_01_08_425864 454 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 454 20 preprint preprint NN 10_1101-2021_01_08_425864 454 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 454 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 454 23 682 682 CD 10_1101-2021_01_08_425864 454 24 [ [ -LRB- 10_1101-2021_01_08_425864 454 25 7 7 CD 10_1101-2021_01_08_425864 454 26 ] ] -RRB- 10_1101-2021_01_08_425864 454 27 Y. Y. NNP 10_1101-2021_01_08_425864 454 28 Li Li NNP 10_1101-2021_01_08_425864 454 29 , , , 10_1101-2021_01_08_425864 454 30 S. S. NNP 10_1101-2021_01_08_425864 454 31 Shah Shah NNP 10_1101-2021_01_08_425864 454 32 - - HYPH 10_1101-2021_01_08_425864 454 33 Simpson Simpson NNP 10_1101-2021_01_08_425864 454 34 , , , 10_1101-2021_01_08_425864 454 35 K. K. NNP 10_1101-2021_01_08_425864 454 36 Okrah Okrah NNP 10_1101-2021_01_08_425864 454 37 , , , 10_1101-2021_01_08_425864 454 38 A.T. A.T. NNP 10_1101-2021_01_08_425864 454 39 Belew Belew NNP 10_1101-2021_01_08_425864 454 40 , , , 10_1101-2021_01_08_425864 454 41 J. J. NNP 10_1101-2021_01_08_425864 454 42 Choi Choi NNP 10_1101-2021_01_08_425864 454 43 , , , 10_1101-2021_01_08_425864 454 44 K.L. K.L. NNP 10_1101-2021_01_08_425864 455 1 Caradonna Caradonna NNP 10_1101-2021_01_08_425864 455 2 , , , 10_1101-2021_01_08_425864 455 3 P. P. NNP 10_1101-2021_01_08_425864 455 4 Padmanabhan Padmanabhan NNP 10_1101-2021_01_08_425864 455 5 , , , 10_1101-2021_01_08_425864 455 6 683 683 CD 10_1101-2021_01_08_425864 455 7 D.M. D.M. NNP 10_1101-2021_01_08_425864 456 1 Ndegwa Ndegwa NNP 10_1101-2021_01_08_425864 456 2 , , , 10_1101-2021_01_08_425864 456 3 M.R. M.R. NNP 10_1101-2021_01_08_425864 457 1 Temanni Temanni NNP 10_1101-2021_01_08_425864 457 2 , , , 10_1101-2021_01_08_425864 457 3 H. H. NNP 10_1101-2021_01_08_425864 457 4 Corrada Corrada NNP 10_1101-2021_01_08_425864 457 5 Bravo Bravo NNP 10_1101-2021_01_08_425864 457 6 , , , 10_1101-2021_01_08_425864 457 7 N.M. New Mexico NNP 10_1101-2021_01_08_425864 458 1 El El NNP 10_1101-2021_01_08_425864 458 2 - - HYPH 10_1101-2021_01_08_425864 458 3 Sayed Sayed NNP 10_1101-2021_01_08_425864 458 4 , , , 10_1101-2021_01_08_425864 458 5 B.A. B.A. NNP 10_1101-2021_01_08_425864 459 1 Burleigh Burleigh NNP 10_1101-2021_01_08_425864 459 2 , , , 10_1101-2021_01_08_425864 459 3 684 684 CD 10_1101-2021_01_08_425864 459 4 Transcriptome Transcriptome NNP 10_1101-2021_01_08_425864 459 5 remodeling remodeling NN 10_1101-2021_01_08_425864 459 6 in in IN 10_1101-2021_01_08_425864 459 7 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 459 8 cruzi cruzi NNS 10_1101-2021_01_08_425864 459 9 and and CC 10_1101-2021_01_08_425864 459 10 human human JJ 10_1101-2021_01_08_425864 459 11 cells cell NNS 10_1101-2021_01_08_425864 459 12 during during IN 10_1101-2021_01_08_425864 459 13 intracellular intracellular JJ 10_1101-2021_01_08_425864 459 14 685 685 CD 10_1101-2021_01_08_425864 459 15 infection infection NN 10_1101-2021_01_08_425864 459 16 , , , 10_1101-2021_01_08_425864 459 17 PLOS PLOS NNP 10_1101-2021_01_08_425864 459 18 Pathog Pathog NNP 10_1101-2021_01_08_425864 459 19 . . . 10_1101-2021_01_08_425864 460 1 12 12 CD 10_1101-2021_01_08_425864 460 2 ( ( -LRB- 10_1101-2021_01_08_425864 460 3 2016 2016 CD 10_1101-2021_01_08_425864 460 4 ) ) -RRB- 10_1101-2021_01_08_425864 460 5 e1005511 e1005511 NNP 10_1101-2021_01_08_425864 460 6 . . . 10_1101-2021_01_08_425864 461 1 https://doi.org/10.1371/journal.ppat.1005511 https://doi.org/10.1371/journal.ppat.1005511 NNP 10_1101-2021_01_08_425864 461 2 . . . 10_1101-2021_01_08_425864 462 1 686 686 CD 10_1101-2021_01_08_425864 462 2 [ [ -LRB- 10_1101-2021_01_08_425864 462 3 8 8 CD 10_1101-2021_01_08_425864 462 4 ] ] -RRB- 10_1101-2021_01_08_425864 462 5 L. L. NNP 10_1101-2021_01_08_425864 462 6 Marchese Marchese NNP 10_1101-2021_01_08_425864 462 7 , , , 10_1101-2021_01_08_425864 462 8 J. J. NNP 10_1101-2021_01_08_425864 462 9 Nascimento Nascimento NNP 10_1101-2021_01_08_425864 462 10 , , , 10_1101-2021_01_08_425864 462 11 F. F. NNP 10_1101-2021_01_08_425864 462 12 Damasceno Damasceno NNP 10_1101-2021_01_08_425864 462 13 , , , 10_1101-2021_01_08_425864 462 14 F. F. NNP 10_1101-2021_01_08_425864 462 15 Bringaud Bringaud NNP 10_1101-2021_01_08_425864 462 16 , , , 10_1101-2021_01_08_425864 462 17 P. P. NNP 10_1101-2021_01_08_425864 462 18 Michels Michels NNP 10_1101-2021_01_08_425864 462 19 , , , 10_1101-2021_01_08_425864 462 20 A. A. NNP 10_1101-2021_01_08_425864 462 21 Silber Silber NNP 10_1101-2021_01_08_425864 462 22 , , , 10_1101-2021_01_08_425864 462 23 The the DT 10_1101-2021_01_08_425864 462 24 uptake uptake JJ 10_1101-2021_01_08_425864 462 25 687 687 CD 10_1101-2021_01_08_425864 462 26 and and CC 10_1101-2021_01_08_425864 462 27 metabolism metabolism NN 10_1101-2021_01_08_425864 462 28 of of IN 10_1101-2021_01_08_425864 462 29 amino amino JJ 10_1101-2021_01_08_425864 462 30 acids acid NNS 10_1101-2021_01_08_425864 462 31 , , , 10_1101-2021_01_08_425864 462 32 and and CC 10_1101-2021_01_08_425864 462 33 their -PRON- PRP$ 10_1101-2021_01_08_425864 462 34 unique unique JJ 10_1101-2021_01_08_425864 462 35 role role NN 10_1101-2021_01_08_425864 462 36 in in IN 10_1101-2021_01_08_425864 462 37 the the DT 10_1101-2021_01_08_425864 462 38 biology biology NN 10_1101-2021_01_08_425864 462 39 of of IN 10_1101-2021_01_08_425864 462 40 pathogenic pathogenic JJ 10_1101-2021_01_08_425864 462 41 688 688 CD 10_1101-2021_01_08_425864 462 42 trypanosomatids trypanosomatid NNS 10_1101-2021_01_08_425864 462 43 , , , 10_1101-2021_01_08_425864 462 44 Pathogens Pathogens NNP 10_1101-2021_01_08_425864 462 45 . . . 10_1101-2021_01_08_425864 463 1 7 7 CD 10_1101-2021_01_08_425864 463 2 ( ( -LRB- 10_1101-2021_01_08_425864 463 3 2018 2018 CD 10_1101-2021_01_08_425864 463 4 ) ) -RRB- 10_1101-2021_01_08_425864 463 5 36 36 CD 10_1101-2021_01_08_425864 463 6 . . . 10_1101-2021_01_08_425864 463 7 https://doi.org/10.3390/pathogens7020036 https://doi.org/10.3390/pathogens7020036 ADD 10_1101-2021_01_08_425864 463 8 . . . 10_1101-2021_01_08_425864 464 1 689 689 CD 10_1101-2021_01_08_425864 464 2 [ [ -LRB- 10_1101-2021_01_08_425864 464 3 9 9 CD 10_1101-2021_01_08_425864 464 4 ] ] -RRB- 10_1101-2021_01_08_425864 464 5 J.J. J.J. NNP 10_1101-2021_01_08_425864 464 6 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 464 7 , , , 10_1101-2021_01_08_425864 464 8 Energy Energy NNP 10_1101-2021_01_08_425864 464 9 metabolism metabolism NN 10_1101-2021_01_08_425864 464 10 in in IN 10_1101-2021_01_08_425864 464 11 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 464 12 cruzi cruzi NNS 10_1101-2021_01_08_425864 464 13 , , , 10_1101-2021_01_08_425864 464 14 Subcell Subcell NNP 10_1101-2021_01_08_425864 464 15 Biochem Biochem NNP 10_1101-2021_01_08_425864 464 16 . . . 10_1101-2021_01_08_425864 465 1 18 18 CD 10_1101-2021_01_08_425864 465 2 ( ( -LRB- 10_1101-2021_01_08_425864 465 3 1992 1992 CD 10_1101-2021_01_08_425864 465 4 ) ) -RRB- 10_1101-2021_01_08_425864 465 5 235 235 CD 10_1101-2021_01_08_425864 465 6 – – : 10_1101-2021_01_08_425864 465 7 690 690 CD 10_1101-2021_01_08_425864 465 8 257 257 CD 10_1101-2021_01_08_425864 465 9 . . . 10_1101-2021_01_08_425864 465 10 https://www.ncbi.nlm.nih.gov/pubmed/1485353 https://www.ncbi.nlm.nih.gov/pubmed/1485353 ADD 10_1101-2021_01_08_425864 465 11 . . . 10_1101-2021_01_08_425864 466 1 691 691 CD 10_1101-2021_01_08_425864 466 2 [ [ -LRB- 10_1101-2021_01_08_425864 466 3 10 10 CD 10_1101-2021_01_08_425864 466 4 ] ] -RRB- 10_1101-2021_01_08_425864 466 5 M.J. M.J. NNP 10_1101-2021_01_08_425864 466 6 Barison Barison NNP 10_1101-2021_01_08_425864 466 7 , , , 10_1101-2021_01_08_425864 466 8 L.N. L.N. NNP 10_1101-2021_01_08_425864 467 1 Rapado Rapado NNP 10_1101-2021_01_08_425864 467 2 , , , 10_1101-2021_01_08_425864 467 3 E.F. E.F. NNP 10_1101-2021_01_08_425864 467 4 Merino Merino NNP 10_1101-2021_01_08_425864 467 5 , , , 10_1101-2021_01_08_425864 467 6 E.M. E.M. NNP 10_1101-2021_01_08_425864 467 7 Furusho Furusho NNP 10_1101-2021_01_08_425864 467 8 Pral Pral NNP 10_1101-2021_01_08_425864 467 9 , , , 10_1101-2021_01_08_425864 467 10 B.S. B.S. NNP 10_1101-2021_01_08_425864 468 1 Mantilla Mantilla NNP 10_1101-2021_01_08_425864 468 2 , , , 10_1101-2021_01_08_425864 468 3 L. L. NNP 10_1101-2021_01_08_425864 468 4 Marchese Marchese NNP 10_1101-2021_01_08_425864 468 5 , , , 10_1101-2021_01_08_425864 468 6 C. C. NNP 10_1101-2021_01_08_425864 468 7 692 692 CD 10_1101-2021_01_08_425864 468 8 Nowicki Nowicki NNP 10_1101-2021_01_08_425864 468 9 , , , 10_1101-2021_01_08_425864 468 10 A.M. A.M. NNP 10_1101-2021_01_08_425864 468 11 Silber Silber NNP 10_1101-2021_01_08_425864 468 12 , , , 10_1101-2021_01_08_425864 468 13 M.B. M.B. NNP 10_1101-2021_01_08_425864 469 1 Cassera Cassera NNP 10_1101-2021_01_08_425864 469 2 , , , 10_1101-2021_01_08_425864 469 3 Metabolomic metabolomic JJ 10_1101-2021_01_08_425864 469 4 profiling profiling NN 10_1101-2021_01_08_425864 469 5 reveals reveal VBZ 10_1101-2021_01_08_425864 469 6 a a DT 10_1101-2021_01_08_425864 469 7 finely finely RB 10_1101-2021_01_08_425864 469 8 tuned tune VBN 10_1101-2021_01_08_425864 469 9 , , , 10_1101-2021_01_08_425864 469 10 693 693 CD 10_1101-2021_01_08_425864 469 11 starvation starvation NN 10_1101-2021_01_08_425864 469 12 - - HYPH 10_1101-2021_01_08_425864 469 13 induced induce VBN 10_1101-2021_01_08_425864 469 14 metabolic metabolic JJ 10_1101-2021_01_08_425864 469 15 switch switch NN 10_1101-2021_01_08_425864 469 16 in in IN 10_1101-2021_01_08_425864 469 17 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 469 18 cruzi cruzi NNS 10_1101-2021_01_08_425864 469 19 epimastigotes epimastigote VBZ 10_1101-2021_01_08_425864 469 20 , , , 10_1101-2021_01_08_425864 469 21 J J NNP 10_1101-2021_01_08_425864 469 22 Biol Biol NNP 10_1101-2021_01_08_425864 469 23 Chem Chem NNP 10_1101-2021_01_08_425864 469 24 . . . 10_1101-2021_01_08_425864 470 1 292 292 CD 10_1101-2021_01_08_425864 470 2 694 694 CD 10_1101-2021_01_08_425864 470 3 ( ( -LRB- 10_1101-2021_01_08_425864 470 4 2017 2017 CD 10_1101-2021_01_08_425864 470 5 ) ) -RRB- 10_1101-2021_01_08_425864 470 6 8964–8977 8964–8977 CD 10_1101-2021_01_08_425864 470 7 . . . 10_1101-2021_01_08_425864 471 1 https://doi.org/10.1074/jbc.M117.778522 https://doi.org/10.1074/jbc.M117.778522 NNP 10_1101-2021_01_08_425864 471 2 . . . 10_1101-2021_01_08_425864 472 1 695 695 CD 10_1101-2021_01_08_425864 472 2 [ [ -LRB- 10_1101-2021_01_08_425864 472 3 11 11 CD 10_1101-2021_01_08_425864 472 4 ] ] -RRB- 10_1101-2021_01_08_425864 472 5 R. R. NNP 10_1101-2021_01_08_425864 472 6 Zeledon Zeledon NNP 10_1101-2021_01_08_425864 472 7 , , , 10_1101-2021_01_08_425864 472 8 Comparative comparative JJ 10_1101-2021_01_08_425864 472 9 physiological physiological JJ 10_1101-2021_01_08_425864 472 10 studies study NNS 10_1101-2021_01_08_425864 472 11 on on IN 10_1101-2021_01_08_425864 472 12 four four CD 10_1101-2021_01_08_425864 472 13 species specie NNS 10_1101-2021_01_08_425864 472 14 of of IN 10_1101-2021_01_08_425864 472 15 hemoflagellates hemoflagellate NNS 10_1101-2021_01_08_425864 472 16 in in IN 10_1101-2021_01_08_425864 472 17 culture culture NN 10_1101-2021_01_08_425864 472 18 . . . 10_1101-2021_01_08_425864 473 1 696 696 CD 10_1101-2021_01_08_425864 473 2 II ii CD 10_1101-2021_01_08_425864 473 3 . . . 10_1101-2021_01_08_425864 474 1 Effect effect NN 10_1101-2021_01_08_425864 474 2 of of IN 10_1101-2021_01_08_425864 474 3 carbohydrates carbohydrate NNS 10_1101-2021_01_08_425864 474 4 and and CC 10_1101-2021_01_08_425864 474 5 related related JJ 10_1101-2021_01_08_425864 474 6 substances substance NNS 10_1101-2021_01_08_425864 474 7 and and CC 10_1101-2021_01_08_425864 474 8 some some DT 10_1101-2021_01_08_425864 474 9 amino amino JJ 10_1101-2021_01_08_425864 474 10 compounds compound NNS 10_1101-2021_01_08_425864 474 11 on on IN 10_1101-2021_01_08_425864 474 12 the the DT 10_1101-2021_01_08_425864 474 13 697 697 CD 10_1101-2021_01_08_425864 474 14 respiration respiration NN 10_1101-2021_01_08_425864 474 15 , , , 10_1101-2021_01_08_425864 474 16 J. J. NNP 10_1101-2021_01_08_425864 474 17 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 474 18 . . . 10_1101-2021_01_08_425864 475 1 46 46 CD 10_1101-2021_01_08_425864 475 2 ( ( -LRB- 10_1101-2021_01_08_425864 475 3 1960 1960 CD 10_1101-2021_01_08_425864 475 4 ) ) -RRB- 10_1101-2021_01_08_425864 475 5 541 541 CD 10_1101-2021_01_08_425864 475 6 . . . 10_1101-2021_01_08_425864 475 7 https://doi.org/10.2307/3274935 https://doi.org/10.2307/3274935 NNP 10_1101-2021_01_08_425864 475 8 . . . 10_1101-2021_01_08_425864 476 1 698 698 CD 10_1101-2021_01_08_425864 476 2 [ [ -LRB- 10_1101-2021_01_08_425864 476 3 12 12 CD 10_1101-2021_01_08_425864 476 4 ] ] -RRB- 10_1101-2021_01_08_425864 476 5 D. D. NNP 10_1101-2021_01_08_425864 476 6 Sylvester Sylvester NNP 10_1101-2021_01_08_425864 476 7 , , , 10_1101-2021_01_08_425864 476 8 S.M. S.M. NNP 10_1101-2021_01_08_425864 477 1 Krassner Krassner NNP 10_1101-2021_01_08_425864 477 2 , , , 10_1101-2021_01_08_425864 477 3 Proline Proline NNP 10_1101-2021_01_08_425864 477 4 metabolism metabolism NN 10_1101-2021_01_08_425864 477 5 in in IN 10_1101-2021_01_08_425864 477 6 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 477 7 cruzi cruzi NNS 10_1101-2021_01_08_425864 477 8 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 477 9 , , , 10_1101-2021_01_08_425864 477 10 Comp Comp NNP 10_1101-2021_01_08_425864 477 11 699 699 CD 10_1101-2021_01_08_425864 477 12 Biochem Biochem NNP 10_1101-2021_01_08_425864 477 13 Physiol Physiol NNP 10_1101-2021_01_08_425864 477 14 B. B. NNP 10_1101-2021_01_08_425864 478 1 55 55 CD 10_1101-2021_01_08_425864 478 2 ( ( -LRB- 10_1101-2021_01_08_425864 478 3 1976 1976 CD 10_1101-2021_01_08_425864 478 4 ) ) -RRB- 10_1101-2021_01_08_425864 478 5 443–447 443–447 CD 10_1101-2021_01_08_425864 478 6 . . . 10_1101-2021_01_08_425864 479 1 https://www.ncbi.nlm.nih.gov/pubmed/789007 https://www.ncbi.nlm.nih.gov/pubmed/789007 ADD 10_1101-2021_01_08_425864 479 2 . . . 10_1101-2021_01_08_425864 480 1 700 700 CD 10_1101-2021_01_08_425864 480 2 [ [ -LRB- 10_1101-2021_01_08_425864 480 3 13 13 CD 10_1101-2021_01_08_425864 480 4 ] ] -RRB- 10_1101-2021_01_08_425864 480 5 L.S. L.S. NNP 10_1101-2021_01_08_425864 481 1 Paes Paes NNP 10_1101-2021_01_08_425864 481 2 , , , 10_1101-2021_01_08_425864 481 3 B. B. NNP 10_1101-2021_01_08_425864 481 4 Suarez Suarez NNP 10_1101-2021_01_08_425864 481 5 Mantilla Mantilla NNP 10_1101-2021_01_08_425864 481 6 , , , 10_1101-2021_01_08_425864 481 7 F.M. F.M. NNP 10_1101-2021_01_08_425864 482 1 Zimbres Zimbres NNP 10_1101-2021_01_08_425864 482 2 , , , 10_1101-2021_01_08_425864 482 3 E.M. E.M. NNP 10_1101-2021_01_08_425864 482 4 Pral Pral NNP 10_1101-2021_01_08_425864 482 5 , , , 10_1101-2021_01_08_425864 482 6 P. P. NNP 10_1101-2021_01_08_425864 482 7 Diogo Diogo NNP 10_1101-2021_01_08_425864 482 8 de de NNP 10_1101-2021_01_08_425864 482 9 Melo Melo NNP 10_1101-2021_01_08_425864 482 10 , , , 10_1101-2021_01_08_425864 482 11 E.B. E.B. NNP 10_1101-2021_01_08_425864 483 1 Tahara Tahara NNP 10_1101-2021_01_08_425864 483 2 , , , 10_1101-2021_01_08_425864 483 3 A.J. A.J. NNP 10_1101-2021_01_08_425864 484 1 701 701 CD 10_1101-2021_01_08_425864 484 2 Kowaltowski Kowaltowski NNPS 10_1101-2021_01_08_425864 484 3 , , , 10_1101-2021_01_08_425864 484 4 M.C. M.C. NNP 10_1101-2021_01_08_425864 485 1 Elias elias RB 10_1101-2021_01_08_425864 485 2 , , , 10_1101-2021_01_08_425864 485 3 A.M. A.M. NNP 10_1101-2021_01_08_425864 485 4 Silber Silber NNP 10_1101-2021_01_08_425864 485 5 , , , 10_1101-2021_01_08_425864 485 6 Proline Proline NNP 10_1101-2021_01_08_425864 485 7 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 485 8 regulates regulate VBZ 10_1101-2021_01_08_425864 485 9 redox redox JJ 10_1101-2021_01_08_425864 485 10 state state NN 10_1101-2021_01_08_425864 485 11 and and CC 10_1101-2021_01_08_425864 485 12 702 702 CD 10_1101-2021_01_08_425864 485 13 respiratory respiratory JJ 10_1101-2021_01_08_425864 485 14 metabolism metabolism NN 10_1101-2021_01_08_425864 485 15 in in IN 10_1101-2021_01_08_425864 485 16 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 485 17 cruzi cruzi NNS 10_1101-2021_01_08_425864 485 18 , , , 10_1101-2021_01_08_425864 485 19 PLoS PLoS NNP 10_1101-2021_01_08_425864 485 20 One one CD 10_1101-2021_01_08_425864 485 21 . . . 10_1101-2021_01_08_425864 486 1 8 8 CD 10_1101-2021_01_08_425864 486 2 ( ( -LRB- 10_1101-2021_01_08_425864 486 3 2013 2013 CD 10_1101-2021_01_08_425864 486 4 ) ) -RRB- 10_1101-2021_01_08_425864 486 5 e69419 e69419 NNP 10_1101-2021_01_08_425864 486 6 . . . 10_1101-2021_01_08_425864 487 1 703 703 CD 10_1101-2021_01_08_425864 487 2 https://doi.org/10.1371/journal.pone.0069419 https://doi.org/10.1371/journal.pone.0069419 NN 10_1101-2021_01_08_425864 487 3 . . . 10_1101-2021_01_08_425864 488 1 704 704 CD 10_1101-2021_01_08_425864 488 2 [ [ -LRB- 10_1101-2021_01_08_425864 488 3 14 14 CD 10_1101-2021_01_08_425864 488 4 ] ] -RRB- 10_1101-2021_01_08_425864 488 5 B.S. B.S. NNP 10_1101-2021_01_08_425864 489 1 Mantilla Mantilla NNP 10_1101-2021_01_08_425864 489 2 , , , 10_1101-2021_01_08_425864 489 3 L.S. L.S. NNP 10_1101-2021_01_08_425864 490 1 Paes Paes NNP 10_1101-2021_01_08_425864 490 2 , , , 10_1101-2021_01_08_425864 490 3 E.M.F. E.M.F. NNP 10_1101-2021_01_08_425864 491 1 Pral pral JJ 10_1101-2021_01_08_425864 491 2 , , , 10_1101-2021_01_08_425864 491 3 D.E. D.E. NNP 10_1101-2021_01_08_425864 492 1 Martil Martil NNP 10_1101-2021_01_08_425864 492 2 , , , 10_1101-2021_01_08_425864 492 3 O.H. O.H. NNP 10_1101-2021_01_08_425864 493 1 Thiemann Thiemann NNP 10_1101-2021_01_08_425864 493 2 , , , 10_1101-2021_01_08_425864 493 3 P. P. NNP 10_1101-2021_01_08_425864 493 4 Fernández Fernández NNP 10_1101-2021_01_08_425864 493 5 - - HYPH 10_1101-2021_01_08_425864 493 6 Silva Silva NNP 10_1101-2021_01_08_425864 493 7 , , , 10_1101-2021_01_08_425864 493 8 E.L. E.L. NNP 10_1101-2021_01_08_425864 493 9 705 705 CD 10_1101-2021_01_08_425864 493 10 Bastos Bastos NNP 10_1101-2021_01_08_425864 493 11 , , , 10_1101-2021_01_08_425864 493 12 A.M. A.M. NNP 10_1101-2021_01_08_425864 493 13 Silber Silber NNP 10_1101-2021_01_08_425864 493 14 , , , 10_1101-2021_01_08_425864 493 15 Role Role NNP 10_1101-2021_01_08_425864 493 16 of of IN 10_1101-2021_01_08_425864 493 17 Δ1-pyrroline-5-carboxylate δ1-pyrroline-5-carboxylate JJ 10_1101-2021_01_08_425864 493 18 dehydrogenase dehydrogenase NN 10_1101-2021_01_08_425864 493 19 supports support VBZ 10_1101-2021_01_08_425864 493 20 706 706 CD 10_1101-2021_01_08_425864 493 21 mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 493 22 metabolism metabolism NN 10_1101-2021_01_08_425864 493 23 and and CC 10_1101-2021_01_08_425864 493 24 host host NN 10_1101-2021_01_08_425864 493 25 - - HYPH 10_1101-2021_01_08_425864 493 26 cell cell NN 10_1101-2021_01_08_425864 493 27 invasion invasion NN 10_1101-2021_01_08_425864 493 28 of of IN 10_1101-2021_01_08_425864 493 29 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 493 30 cruzi cruzi NNS 10_1101-2021_01_08_425864 493 31 . . . 10_1101-2021_01_08_425864 493 32 , , , 10_1101-2021_01_08_425864 493 33 J. J. NNP 10_1101-2021_01_08_425864 493 34 Biol Biol NNP 10_1101-2021_01_08_425864 493 35 . . . 10_1101-2021_01_08_425864 494 1 Chem Chem NNP 10_1101-2021_01_08_425864 494 2 . . . 10_1101-2021_01_08_425864 495 1 290 290 CD 10_1101-2021_01_08_425864 495 2 707 707 CD 10_1101-2021_01_08_425864 495 3 ( ( -LRB- 10_1101-2021_01_08_425864 495 4 2015 2015 CD 10_1101-2021_01_08_425864 495 5 ) ) -RRB- 10_1101-2021_01_08_425864 495 6 . . . 10_1101-2021_01_08_425864 496 1 https://doi.org/10.1074/jbc.M114.574525 https://doi.org/10.1074/jbc.M114.574525 NNP 10_1101-2021_01_08_425864 496 2 . . . 10_1101-2021_01_08_425864 497 1 .CC .CC NFP 10_1101-2021_01_08_425864 497 2 - - : 10_1101-2021_01_08_425864 497 3 BY by IN 10_1101-2021_01_08_425864 497 4 4.0 4.0 CD 10_1101-2021_01_08_425864 497 5 International international JJ 10_1101-2021_01_08_425864 497 6 licensemade licensemade NN 10_1101-2021_01_08_425864 497 7 available available JJ 10_1101-2021_01_08_425864 497 8 under under IN 10_1101-2021_01_08_425864 497 9 a a DT 10_1101-2021_01_08_425864 497 10 ( ( -LRB- 10_1101-2021_01_08_425864 497 11 which which WDT 10_1101-2021_01_08_425864 497 12 was be VBD 10_1101-2021_01_08_425864 497 13 not not RB 10_1101-2021_01_08_425864 497 14 certified certify VBN 10_1101-2021_01_08_425864 497 15 by by IN 10_1101-2021_01_08_425864 497 16 peer peer NN 10_1101-2021_01_08_425864 497 17 review review NN 10_1101-2021_01_08_425864 497 18 ) ) -RRB- 10_1101-2021_01_08_425864 497 19 is be VBZ 10_1101-2021_01_08_425864 497 20 the the DT 10_1101-2021_01_08_425864 497 21 author author NN 10_1101-2021_01_08_425864 497 22 / / SYM 10_1101-2021_01_08_425864 497 23 funder funder NN 10_1101-2021_01_08_425864 497 24 , , , 10_1101-2021_01_08_425864 497 25 who who WP 10_1101-2021_01_08_425864 497 26 has have VBZ 10_1101-2021_01_08_425864 497 27 granted grant VBN 10_1101-2021_01_08_425864 497 28 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 497 29 a a DT 10_1101-2021_01_08_425864 497 30 license license NN 10_1101-2021_01_08_425864 497 31 to to TO 10_1101-2021_01_08_425864 497 32 display display VB 10_1101-2021_01_08_425864 497 33 the the DT 10_1101-2021_01_08_425864 497 34 preprint preprint NN 10_1101-2021_01_08_425864 497 35 in in IN 10_1101-2021_01_08_425864 497 36 perpetuity perpetuity NN 10_1101-2021_01_08_425864 497 37 . . . 10_1101-2021_01_08_425864 498 1 It -PRON- PRP 10_1101-2021_01_08_425864 498 2 is be VBZ 10_1101-2021_01_08_425864 498 3 The the DT 10_1101-2021_01_08_425864 498 4 copyright copyright NN 10_1101-2021_01_08_425864 498 5 holder holder NN 10_1101-2021_01_08_425864 498 6 for for IN 10_1101-2021_01_08_425864 498 7 this this DT 10_1101-2021_01_08_425864 498 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 498 9 version version NN 10_1101-2021_01_08_425864 498 10 posted post VBD 10_1101-2021_01_08_425864 498 11 January January NNP 10_1101-2021_01_08_425864 498 12 8 8 CD 10_1101-2021_01_08_425864 498 13 , , , 10_1101-2021_01_08_425864 498 14 2021 2021 CD 10_1101-2021_01_08_425864 498 15 . . . 10_1101-2021_01_08_425864 498 16 ; ; : 10_1101-2021_01_08_425864 498 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 498 18 : : : 10_1101-2021_01_08_425864 498 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 498 20 preprint preprint NN 10_1101-2021_01_08_425864 498 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 498 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 498 23 708 708 CD 10_1101-2021_01_08_425864 498 24 [ [ -LRB- 10_1101-2021_01_08_425864 498 25 15 15 CD 10_1101-2021_01_08_425864 498 26 ] ] -RRB- 10_1101-2021_01_08_425864 498 27 M.J. M.J. NNP 10_1101-2021_01_08_425864 498 28 Barisón Barisón NNP 10_1101-2021_01_08_425864 498 29 , , , 10_1101-2021_01_08_425864 498 30 F.S. F.S. NNP 10_1101-2021_01_08_425864 499 1 Damasceno Damasceno NNP 10_1101-2021_01_08_425864 499 2 , , , 10_1101-2021_01_08_425864 499 3 B.S. B.S. NNP 10_1101-2021_01_08_425864 500 1 Mantilla Mantilla NNP 10_1101-2021_01_08_425864 500 2 , , , 10_1101-2021_01_08_425864 500 3 A.M. A.M. NNP 10_1101-2021_01_08_425864 500 4 Silber Silber NNP 10_1101-2021_01_08_425864 500 5 , , , 10_1101-2021_01_08_425864 500 6 The the DT 10_1101-2021_01_08_425864 500 7 active active JJ 10_1101-2021_01_08_425864 500 8 transport transport NN 10_1101-2021_01_08_425864 500 9 of of IN 10_1101-2021_01_08_425864 500 10 histidine histidine NNP 10_1101-2021_01_08_425864 500 11 709 709 CD 10_1101-2021_01_08_425864 500 12 and and CC 10_1101-2021_01_08_425864 500 13 its -PRON- PRP$ 10_1101-2021_01_08_425864 500 14 role role NN 10_1101-2021_01_08_425864 500 15 in in IN 10_1101-2021_01_08_425864 500 16 ATP atp NN 10_1101-2021_01_08_425864 500 17 production production NN 10_1101-2021_01_08_425864 500 18 in in IN 10_1101-2021_01_08_425864 500 19 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 500 20 cruzi cruzi NNS 10_1101-2021_01_08_425864 500 21 . . . 10_1101-2021_01_08_425864 500 22 , , , 10_1101-2021_01_08_425864 500 23 J. J. NNP 10_1101-2021_01_08_425864 500 24 Bioenerg Bioenerg NNP 10_1101-2021_01_08_425864 500 25 . . . 10_1101-2021_01_08_425864 501 1 Biomembr Biomembr NNP 10_1101-2021_01_08_425864 501 2 . . . 10_1101-2021_01_08_425864 502 1 48 48 CD 10_1101-2021_01_08_425864 502 2 ( ( -LRB- 10_1101-2021_01_08_425864 502 3 2016 2016 CD 10_1101-2021_01_08_425864 502 4 ) ) -RRB- 10_1101-2021_01_08_425864 502 5 710 710 CD 10_1101-2021_01_08_425864 502 6 437–49 437–49 CD 10_1101-2021_01_08_425864 502 7 . . . 10_1101-2021_01_08_425864 503 1 https://doi.org/10.1007/s10863-016-9665-9 https://doi.org/10.1007/s10863-016-9665-9 ADD 10_1101-2021_01_08_425864 503 2 . . . 10_1101-2021_01_08_425864 504 1 711 711 CD 10_1101-2021_01_08_425864 504 2 [ [ -LRB- 10_1101-2021_01_08_425864 504 3 16 16 CD 10_1101-2021_01_08_425864 504 4 ] ] -RRB- 10_1101-2021_01_08_425864 504 5 R.M.B.M. R.M.B.M. NNP 10_1101-2021_01_08_425864 505 1 Girard Girard NNP 10_1101-2021_01_08_425864 505 2 , , , 10_1101-2021_01_08_425864 505 3 M. M. NNP 10_1101-2021_01_08_425864 505 4 Crispim Crispim NNP 10_1101-2021_01_08_425864 505 5 , , , 10_1101-2021_01_08_425864 505 6 M.B. M.B. NNP 10_1101-2021_01_08_425864 506 1 Alencar Alencar NNP 10_1101-2021_01_08_425864 506 2 , , , 10_1101-2021_01_08_425864 506 3 A.M. A.M. NNP 10_1101-2021_01_08_425864 506 4 Silber Silber NNP 10_1101-2021_01_08_425864 506 5 , , , 10_1101-2021_01_08_425864 506 6 Uptake Uptake NNP 10_1101-2021_01_08_425864 506 7 of of IN 10_1101-2021_01_08_425864 506 8 L L NNP 10_1101-2021_01_08_425864 506 9 - - HYPH 10_1101-2021_01_08_425864 506 10 alanine alanine NNP 10_1101-2021_01_08_425864 506 11 and and CC 10_1101-2021_01_08_425864 506 12 its -PRON- PRP$ 10_1101-2021_01_08_425864 506 13 712 712 CD 10_1101-2021_01_08_425864 506 14 distinct distinct JJ 10_1101-2021_01_08_425864 506 15 roles role NNS 10_1101-2021_01_08_425864 506 16 in in IN 10_1101-2021_01_08_425864 506 17 the the DT 10_1101-2021_01_08_425864 506 18 bioenergetics bioenergetic NNS 10_1101-2021_01_08_425864 506 19 of of IN 10_1101-2021_01_08_425864 506 20 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 506 21 cruzi cruzi NNS 10_1101-2021_01_08_425864 506 22 , , , 10_1101-2021_01_08_425864 506 23 MSphere msphere RB 10_1101-2021_01_08_425864 506 24 . . . 10_1101-2021_01_08_425864 507 1 3 3 CD 10_1101-2021_01_08_425864 507 2 ( ( -LRB- 10_1101-2021_01_08_425864 507 3 2018 2018 CD 10_1101-2021_01_08_425864 507 4 ) ) -RRB- 10_1101-2021_01_08_425864 507 5 . . . 10_1101-2021_01_08_425864 508 1 713 713 CD 10_1101-2021_01_08_425864 508 2 https://doi.org/10.1128/mSphereDirect.00338-18 https://doi.org/10.1128/mSphereDirect.00338-18 NNS 10_1101-2021_01_08_425864 508 3 . . . 10_1101-2021_01_08_425864 509 1 714 714 CD 10_1101-2021_01_08_425864 509 2 [ [ -LRB- 10_1101-2021_01_08_425864 509 3 17 17 CD 10_1101-2021_01_08_425864 509 4 ] ] -RRB- 10_1101-2021_01_08_425864 509 5 F.S. F.S. NNP 10_1101-2021_01_08_425864 510 1 Damasceno Damasceno NNP 10_1101-2021_01_08_425864 510 2 , , , 10_1101-2021_01_08_425864 510 3 M.J. M.J. NNP 10_1101-2021_01_08_425864 510 4 Barisón Barisón NNP 10_1101-2021_01_08_425864 510 5 , , , 10_1101-2021_01_08_425864 510 6 M. M. NNP 10_1101-2021_01_08_425864 510 7 Crispim Crispim NNP 10_1101-2021_01_08_425864 510 8 , , , 10_1101-2021_01_08_425864 510 9 R.O.O. R.O.O. NNP 10_1101-2021_01_08_425864 511 1 Souza Souza NNP 10_1101-2021_01_08_425864 511 2 , , , 10_1101-2021_01_08_425864 511 3 L. L. NNP 10_1101-2021_01_08_425864 511 4 Marchese Marchese NNP 10_1101-2021_01_08_425864 511 5 , , , 10_1101-2021_01_08_425864 511 6 A.M. A.M. NNP 10_1101-2021_01_08_425864 511 7 Silber Silber NNP 10_1101-2021_01_08_425864 511 8 , , , 10_1101-2021_01_08_425864 511 9 L- L- NNP 10_1101-2021_01_08_425864 511 10 715 715 CD 10_1101-2021_01_08_425864 511 11 Glutamine Glutamine NNP 10_1101-2021_01_08_425864 511 12 uptake uptake NN 10_1101-2021_01_08_425864 511 13 is be VBZ 10_1101-2021_01_08_425864 511 14 developmentally developmentally RB 10_1101-2021_01_08_425864 511 15 regulated regulate VBN 10_1101-2021_01_08_425864 511 16 and and CC 10_1101-2021_01_08_425864 511 17 is be VBZ 10_1101-2021_01_08_425864 511 18 involved involve VBN 10_1101-2021_01_08_425864 511 19 in in IN 10_1101-2021_01_08_425864 511 20 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 511 21 in in IN 10_1101-2021_01_08_425864 511 22 716 716 CD 10_1101-2021_01_08_425864 511 23 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 511 24 cruzi cruzi NNS 10_1101-2021_01_08_425864 511 25 , , , 10_1101-2021_01_08_425864 511 26 Mol Mol NNP 10_1101-2021_01_08_425864 511 27 . . . 10_1101-2021_01_08_425864 512 1 Biochem Biochem NNP 10_1101-2021_01_08_425864 512 2 . . . 10_1101-2021_01_08_425864 513 1 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 513 2 . . . 10_1101-2021_01_08_425864 514 1 224 224 CD 10_1101-2021_01_08_425864 514 2 ( ( -LRB- 10_1101-2021_01_08_425864 514 3 2018 2018 CD 10_1101-2021_01_08_425864 514 4 ) ) -RRB- 10_1101-2021_01_08_425864 514 5 . . . 10_1101-2021_01_08_425864 515 1 717 717 CD 10_1101-2021_01_08_425864 515 2 https://doi.org/10.1016/j.molbiopara.2018.07.007 https://doi.org/10.1016/j.molbiopara.2018.07.007 CD 10_1101-2021_01_08_425864 515 3 . . . 10_1101-2021_01_08_425864 516 1 718 718 CD 10_1101-2021_01_08_425864 516 2 [ [ -LRB- 10_1101-2021_01_08_425864 516 3 18 18 CD 10_1101-2021_01_08_425864 516 4 ] ] -RRB- 10_1101-2021_01_08_425864 516 5 E.P. E.P. NNP 10_1101-2021_01_08_425864 517 1 Camargo camargo NN 10_1101-2021_01_08_425864 517 2 , , , 10_1101-2021_01_08_425864 517 3 Growth growth NN 10_1101-2021_01_08_425864 517 4 and and CC 10_1101-2021_01_08_425864 517 5 differentiation differentiation NN 10_1101-2021_01_08_425864 517 6 in in IN 10_1101-2021_01_08_425864 517 7 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 517 8 cruzi cruzi NNS 10_1101-2021_01_08_425864 517 9 . . . 10_1101-2021_01_08_425864 518 1 I. I. NNP 10_1101-2021_01_08_425864 519 1 Origin origin NN 10_1101-2021_01_08_425864 519 2 of of IN 10_1101-2021_01_08_425864 519 3 metacyclic metacyclic JJ 10_1101-2021_01_08_425864 519 4 719 719 CD 10_1101-2021_01_08_425864 519 5 trypanosomes trypanosome NNS 10_1101-2021_01_08_425864 519 6 in in IN 10_1101-2021_01_08_425864 519 7 liquid liquid JJ 10_1101-2021_01_08_425864 519 8 media medium NNS 10_1101-2021_01_08_425864 519 9 , , , 10_1101-2021_01_08_425864 519 10 Rev. Rev. NNP 10_1101-2021_01_08_425864 520 1 Inst inst RB 10_1101-2021_01_08_425864 520 2 . . . 10_1101-2021_01_08_425864 521 1 Med Med NNP 10_1101-2021_01_08_425864 521 2 . . . 10_1101-2021_01_08_425864 522 1 Trop Trop NNP 10_1101-2021_01_08_425864 522 2 . . . 10_1101-2021_01_08_425864 523 1 Sao Sao NNP 10_1101-2021_01_08_425864 523 2 Paulo Paulo NNP 10_1101-2021_01_08_425864 523 3 . . . 10_1101-2021_01_08_425864 524 1 6 6 CD 10_1101-2021_01_08_425864 524 2 ( ( -LRB- 10_1101-2021_01_08_425864 524 3 1964 1964 CD 10_1101-2021_01_08_425864 524 4 ) ) -RRB- 10_1101-2021_01_08_425864 524 5 93–100 93–100 CD 10_1101-2021_01_08_425864 524 6 . . . 10_1101-2021_01_08_425864 525 1 720 720 CD 10_1101-2021_01_08_425864 525 2 http://www.ncbi.nlm.nih.gov/pubmed/14177814 http://www.ncbi.nlm.nih.gov/pubmed/14177814 NNS 10_1101-2021_01_08_425864 525 3 ( ( -LRB- 10_1101-2021_01_08_425864 525 4 accessed access VBN 10_1101-2021_01_08_425864 525 5 January January NNP 10_1101-2021_01_08_425864 525 6 29 29 CD 10_1101-2021_01_08_425864 525 7 , , , 10_1101-2021_01_08_425864 525 8 2019 2019 CD 10_1101-2021_01_08_425864 525 9 ) ) -RRB- 10_1101-2021_01_08_425864 525 10 . . . 10_1101-2021_01_08_425864 526 1 721 721 CD 10_1101-2021_01_08_425864 526 2 [ [ -LRB- 10_1101-2021_01_08_425864 526 3 19 19 CD 10_1101-2021_01_08_425864 526 4 ] ] -RRB- 10_1101-2021_01_08_425864 526 5 F.S. F.S. NNP 10_1101-2021_01_08_425864 527 1 Damasceno Damasceno NNP 10_1101-2021_01_08_425864 527 2 , , , 10_1101-2021_01_08_425864 527 3 M.J. M.J. NNP 10_1101-2021_01_08_425864 527 4 Barison Barison NNP 10_1101-2021_01_08_425864 527 5 , , , 10_1101-2021_01_08_425864 527 6 M. M. NNP 10_1101-2021_01_08_425864 527 7 Crispim Crispim NNP 10_1101-2021_01_08_425864 527 8 , , , 10_1101-2021_01_08_425864 527 9 R.O.O. R.O.O. NNP 10_1101-2021_01_08_425864 528 1 Souza Souza NNP 10_1101-2021_01_08_425864 528 2 , , , 10_1101-2021_01_08_425864 528 3 L. L. NNP 10_1101-2021_01_08_425864 528 4 Marchese Marchese NNP 10_1101-2021_01_08_425864 528 5 , , , 10_1101-2021_01_08_425864 528 6 A.M. A.M. NNP 10_1101-2021_01_08_425864 528 7 Silber Silber NNP 10_1101-2021_01_08_425864 528 8 , , , 10_1101-2021_01_08_425864 528 9 L- L- NNP 10_1101-2021_01_08_425864 528 10 722 722 CD 10_1101-2021_01_08_425864 528 11 Glutamine Glutamine NNP 10_1101-2021_01_08_425864 528 12 uptake uptake NN 10_1101-2021_01_08_425864 528 13 is be VBZ 10_1101-2021_01_08_425864 528 14 developmentally developmentally RB 10_1101-2021_01_08_425864 528 15 regulated regulate VBN 10_1101-2021_01_08_425864 528 16 and and CC 10_1101-2021_01_08_425864 528 17 is be VBZ 10_1101-2021_01_08_425864 528 18 involved involve VBN 10_1101-2021_01_08_425864 528 19 in in IN 10_1101-2021_01_08_425864 528 20 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 528 21 in in IN 10_1101-2021_01_08_425864 528 22 723 723 CD 10_1101-2021_01_08_425864 528 23 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 528 24 cruzi cruzi NNS 10_1101-2021_01_08_425864 528 25 , , , 10_1101-2021_01_08_425864 528 26 Mol Mol NNP 10_1101-2021_01_08_425864 528 27 Biochem Biochem NNP 10_1101-2021_01_08_425864 528 28 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 528 29 . . . 10_1101-2021_01_08_425864 529 1 224 224 CD 10_1101-2021_01_08_425864 529 2 ( ( -LRB- 10_1101-2021_01_08_425864 529 3 2018 2018 CD 10_1101-2021_01_08_425864 529 4 ) ) -RRB- 10_1101-2021_01_08_425864 529 5 17–25 17–25 CD 10_1101-2021_01_08_425864 529 6 . . . 10_1101-2021_01_08_425864 530 1 724 724 CD 10_1101-2021_01_08_425864 530 2 https://doi.org/10.1016/j.molbiopara.2018.07.007 https://doi.org/10.1016/j.molbiopara.2018.07.007 NN 10_1101-2021_01_08_425864 530 3 . . . 10_1101-2021_01_08_425864 531 1 725 725 CD 10_1101-2021_01_08_425864 531 2 [ [ -LRB- 10_1101-2021_01_08_425864 531 3 20 20 CD 10_1101-2021_01_08_425864 531 4 ] ] -RRB- 10_1101-2021_01_08_425864 531 5 F.K. F.K. NNP 10_1101-2021_01_08_425864 532 1 Huynh Huynh NNP 10_1101-2021_01_08_425864 532 2 , , , 10_1101-2021_01_08_425864 532 3 M.F. M.F. NNP 10_1101-2021_01_08_425864 533 1 Green green JJ 10_1101-2021_01_08_425864 533 2 , , , 10_1101-2021_01_08_425864 533 3 T.R. T.R. NNP 10_1101-2021_01_08_425864 534 1 Koves kove NNS 10_1101-2021_01_08_425864 534 2 , , , 10_1101-2021_01_08_425864 534 3 M.D. M.D. NNP 10_1101-2021_01_08_425864 535 1 Hirschey Hirschey NNP 10_1101-2021_01_08_425864 535 2 , , , 10_1101-2021_01_08_425864 535 3 Measurement Measurement NNP 10_1101-2021_01_08_425864 535 4 of of IN 10_1101-2021_01_08_425864 535 5 fatty fatty NN 10_1101-2021_01_08_425864 535 6 acid acid NN 10_1101-2021_01_08_425864 535 7 oxidation oxidation NN 10_1101-2021_01_08_425864 535 8 726 726 CD 10_1101-2021_01_08_425864 535 9 rates rate NNS 10_1101-2021_01_08_425864 535 10 in in IN 10_1101-2021_01_08_425864 535 11 animal animal NN 10_1101-2021_01_08_425864 535 12 tissues tissue NNS 10_1101-2021_01_08_425864 535 13 and and CC 10_1101-2021_01_08_425864 535 14 cell cell NN 10_1101-2021_01_08_425864 535 15 lines line NNS 10_1101-2021_01_08_425864 535 16 . . . 10_1101-2021_01_08_425864 536 1 Methods Methods NNP 10_1101-2021_01_08_425864 536 2 Enzymol Enzymol NNP 10_1101-2021_01_08_425864 536 3 . . . 10_1101-2021_01_08_425864 537 1 542 542 CD 10_1101-2021_01_08_425864 537 2 ( ( -LRB- 10_1101-2021_01_08_425864 537 3 2014 2014 CD 10_1101-2021_01_08_425864 537 4 ) ) -RRB- 10_1101-2021_01_08_425864 537 5 391–405 391–405 CD 10_1101-2021_01_08_425864 537 6 . . . 10_1101-2021_01_08_425864 538 1 727 727 CD 10_1101-2021_01_08_425864 538 2 https://doi.org/10.1016/B978-0-12-416618-9.00020-0 https://doi.org/10.1016/B978-0-12-416618-9.00020-0 NNP 10_1101-2021_01_08_425864 538 3 . . . 10_1101-2021_01_08_425864 539 1 728 728 CD 10_1101-2021_01_08_425864 539 2 [ [ -LRB- 10_1101-2021_01_08_425864 539 3 21 21 CD 10_1101-2021_01_08_425864 539 4 ] ] -RRB- 10_1101-2021_01_08_425864 539 5 M.B. M.B. NNP 10_1101-2021_01_08_425864 540 1 Alencar Alencar NNP 10_1101-2021_01_08_425864 540 2 , , , 10_1101-2021_01_08_425864 540 3 R.B.M.M. R.B.M.M. NNP 10_1101-2021_01_08_425864 541 1 Girard Girard NNP 10_1101-2021_01_08_425864 541 2 , , , 10_1101-2021_01_08_425864 541 3 A.M. A.M. NNP 10_1101-2021_01_08_425864 541 4 Silber Silber NNP 10_1101-2021_01_08_425864 541 5 , , , 10_1101-2021_01_08_425864 541 6 Measurement Measurement NNP 10_1101-2021_01_08_425864 541 7 of of IN 10_1101-2021_01_08_425864 541 8 energy energy NN 10_1101-2021_01_08_425864 541 9 states state NNS 10_1101-2021_01_08_425864 541 10 of of IN 10_1101-2021_01_08_425864 541 11 the the DT 10_1101-2021_01_08_425864 541 12 729 729 CD 10_1101-2021_01_08_425864 541 13 trypanosomatid trypanosomatid NN 10_1101-2021_01_08_425864 541 14 mitochondrion mitochondrion NN 10_1101-2021_01_08_425864 541 15 . . . 10_1101-2021_01_08_425864 542 1 Methods Methods NNPS 10_1101-2021_01_08_425864 542 2 Mol Mol NNP 10_1101-2021_01_08_425864 542 3 . . . 10_1101-2021_01_08_425864 543 1 Biol Biol NNP 10_1101-2021_01_08_425864 543 2 . . . 10_1101-2021_01_08_425864 544 1 2116 2116 CD 10_1101-2021_01_08_425864 544 2 ( ( -LRB- 10_1101-2021_01_08_425864 544 3 2020 2020 CD 10_1101-2021_01_08_425864 544 4 ) ) -RRB- 10_1101-2021_01_08_425864 544 5 655–671 655–671 CD 10_1101-2021_01_08_425864 544 6 . . . 10_1101-2021_01_08_425864 545 1 730 730 CD 10_1101-2021_01_08_425864 545 2 https://doi.org/10.1007/978-1-0716-0294-2_39 https://doi.org/10.1007/978-1-0716-0294-2_39 NNS 10_1101-2021_01_08_425864 545 3 . . . 10_1101-2021_01_08_425864 546 1 731 731 CD 10_1101-2021_01_08_425864 546 2 [ [ -LRB- 10_1101-2021_01_08_425864 546 3 22 22 CD 10_1101-2021_01_08_425864 546 4 ] ] -RRB- 10_1101-2021_01_08_425864 546 5 M.M. M.M. NNP 10_1101-2021_01_08_425864 547 1 Bradford Bradford NNP 10_1101-2021_01_08_425864 547 2 , , , 10_1101-2021_01_08_425864 547 3 A a DT 10_1101-2021_01_08_425864 547 4 rapid rapid JJ 10_1101-2021_01_08_425864 547 5 and and CC 10_1101-2021_01_08_425864 547 6 sensitive sensitive JJ 10_1101-2021_01_08_425864 547 7 method method NN 10_1101-2021_01_08_425864 547 8 for for IN 10_1101-2021_01_08_425864 547 9 the the DT 10_1101-2021_01_08_425864 547 10 quantitation quantitation NN 10_1101-2021_01_08_425864 547 11 of of IN 10_1101-2021_01_08_425864 547 12 microgram microgram NN 10_1101-2021_01_08_425864 547 13 quantities quantity NNS 10_1101-2021_01_08_425864 547 14 of of IN 10_1101-2021_01_08_425864 547 15 732 732 CD 10_1101-2021_01_08_425864 547 16 protein protein NN 10_1101-2021_01_08_425864 547 17 utilizing utilize VBG 10_1101-2021_01_08_425864 547 18 the the DT 10_1101-2021_01_08_425864 547 19 principle principle NN 10_1101-2021_01_08_425864 547 20 of of IN 10_1101-2021_01_08_425864 547 21 protein protein NN 10_1101-2021_01_08_425864 547 22 - - HYPH 10_1101-2021_01_08_425864 547 23 dye dye NN 10_1101-2021_01_08_425864 547 24 binding binding NN 10_1101-2021_01_08_425864 547 25 . . . 10_1101-2021_01_08_425864 548 1 Anal anal JJ 10_1101-2021_01_08_425864 548 2 . . . 10_1101-2021_01_08_425864 549 1 Biochem Biochem NNP 10_1101-2021_01_08_425864 549 2 . . . 10_1101-2021_01_08_425864 550 1 72 72 CD 10_1101-2021_01_08_425864 550 2 ( ( -LRB- 10_1101-2021_01_08_425864 550 3 1976 1976 CD 10_1101-2021_01_08_425864 550 4 ) ) -RRB- 10_1101-2021_01_08_425864 550 5 248–54 248–54 CD 10_1101-2021_01_08_425864 550 6 . . . 10_1101-2021_01_08_425864 551 1 733 733 CD 10_1101-2021_01_08_425864 551 2 https://doi.org/10.1006/abio.1976.9999 https://doi.org/10.1006/abio.1976.9999 JJ 10_1101-2021_01_08_425864 551 3 . . . 10_1101-2021_01_08_425864 552 1 .CC .CC NFP 10_1101-2021_01_08_425864 552 2 - - : 10_1101-2021_01_08_425864 552 3 BY by IN 10_1101-2021_01_08_425864 552 4 4.0 4.0 CD 10_1101-2021_01_08_425864 552 5 International international JJ 10_1101-2021_01_08_425864 552 6 licensemade licensemade NN 10_1101-2021_01_08_425864 552 7 available available JJ 10_1101-2021_01_08_425864 552 8 under under IN 10_1101-2021_01_08_425864 552 9 a a DT 10_1101-2021_01_08_425864 552 10 ( ( -LRB- 10_1101-2021_01_08_425864 552 11 which which WDT 10_1101-2021_01_08_425864 552 12 was be VBD 10_1101-2021_01_08_425864 552 13 not not RB 10_1101-2021_01_08_425864 552 14 certified certify VBN 10_1101-2021_01_08_425864 552 15 by by IN 10_1101-2021_01_08_425864 552 16 peer peer NN 10_1101-2021_01_08_425864 552 17 review review NN 10_1101-2021_01_08_425864 552 18 ) ) -RRB- 10_1101-2021_01_08_425864 552 19 is be VBZ 10_1101-2021_01_08_425864 552 20 the the DT 10_1101-2021_01_08_425864 552 21 author author NN 10_1101-2021_01_08_425864 552 22 / / SYM 10_1101-2021_01_08_425864 552 23 funder funder NN 10_1101-2021_01_08_425864 552 24 , , , 10_1101-2021_01_08_425864 552 25 who who WP 10_1101-2021_01_08_425864 552 26 has have VBZ 10_1101-2021_01_08_425864 552 27 granted grant VBN 10_1101-2021_01_08_425864 552 28 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 552 29 a a DT 10_1101-2021_01_08_425864 552 30 license license NN 10_1101-2021_01_08_425864 552 31 to to TO 10_1101-2021_01_08_425864 552 32 display display VB 10_1101-2021_01_08_425864 552 33 the the DT 10_1101-2021_01_08_425864 552 34 preprint preprint NN 10_1101-2021_01_08_425864 552 35 in in IN 10_1101-2021_01_08_425864 552 36 perpetuity perpetuity NN 10_1101-2021_01_08_425864 552 37 . . . 10_1101-2021_01_08_425864 553 1 It -PRON- PRP 10_1101-2021_01_08_425864 553 2 is be VBZ 10_1101-2021_01_08_425864 553 3 The the DT 10_1101-2021_01_08_425864 553 4 copyright copyright NN 10_1101-2021_01_08_425864 553 5 holder holder NN 10_1101-2021_01_08_425864 553 6 for for IN 10_1101-2021_01_08_425864 553 7 this this DT 10_1101-2021_01_08_425864 553 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 553 9 version version NN 10_1101-2021_01_08_425864 553 10 posted post VBD 10_1101-2021_01_08_425864 553 11 January January NNP 10_1101-2021_01_08_425864 553 12 8 8 CD 10_1101-2021_01_08_425864 553 13 , , , 10_1101-2021_01_08_425864 553 14 2021 2021 CD 10_1101-2021_01_08_425864 553 15 . . . 10_1101-2021_01_08_425864 553 16 ; ; : 10_1101-2021_01_08_425864 553 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 553 18 : : : 10_1101-2021_01_08_425864 553 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 553 20 preprint preprint NN 10_1101-2021_01_08_425864 553 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 553 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 553 23 734 734 CD 10_1101-2021_01_08_425864 553 24 [ [ -LRB- 10_1101-2021_01_08_425864 553 25 23 23 CD 10_1101-2021_01_08_425864 553 26 ] ] -RRB- 10_1101-2021_01_08_425864 553 27 P.L.L. P.L.L. NNP 10_1101-2021_01_08_425864 554 1 Bieber Bieber NNP 10_1101-2021_01_08_425864 554 2 , , , 10_1101-2021_01_08_425864 554 3 T. T. NNP 10_1101-2021_01_08_425864 554 4 Abraham Abraham NNP 10_1101-2021_01_08_425864 554 5 , , , 10_1101-2021_01_08_425864 554 6 T. T. NNP 10_1101-2021_01_08_425864 554 7 Helmrath Helmrath NNP 10_1101-2021_01_08_425864 554 8 , , , 10_1101-2021_01_08_425864 554 9 Y. Y. NNP 10_1101-2021_01_08_425864 554 10 Kim Kim NNP 10_1101-2021_01_08_425864 554 11 , , , 10_1101-2021_01_08_425864 554 12 R. R. NNP 10_1101-2021_01_08_425864 554 13 Dehlin Dehlin NNP 10_1101-2021_01_08_425864 554 14 , , , 10_1101-2021_01_08_425864 554 15 A A NNP 10_1101-2021_01_08_425864 554 16 Rapid Rapid NNP 10_1101-2021_01_08_425864 554 17 Spectrophotometric Spectrophotometric NNP 10_1101-2021_01_08_425864 554 18 735 735 CD 10_1101-2021_01_08_425864 554 19 assay assay NN 10_1101-2021_01_08_425864 554 20 for for IN 10_1101-2021_01_08_425864 554 21 carnitine carnitine NN 10_1101-2021_01_08_425864 554 22 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 554 23 , , , 10_1101-2021_01_08_425864 554 24 1972 1972 CD 10_1101-2021_01_08_425864 554 25 . . . 10_1101-2021_01_08_425864 554 26 https://ac.els-cdn.com/0003269772900619/1- https://ac.els-cdn.com/0003269772900619/1- UH 10_1101-2021_01_08_425864 554 27 736 736 CD 10_1101-2021_01_08_425864 554 28 s2.0-0003269772900619-main.pdf?_tid=d5130378-0890-4a27-8e0e- s2.0-0003269772900619-main.pdf?_tid=d5130378-0890-4a27-8e0e- NNP 10_1101-2021_01_08_425864 554 29 737 737 CD 10_1101-2021_01_08_425864 554 30 63a5c3921132&acdnat=1549722154_d3bdaa5775a2a24b64ccf923a6b8ba4d 63a5c3921132&acdnat=1549722154_d3bdaa5775a2a24b64ccf923a6b8ba4d CD 10_1101-2021_01_08_425864 554 31 ( ( -LRB- 10_1101-2021_01_08_425864 554 32 accessed access VBN 10_1101-2021_01_08_425864 554 33 738 738 CD 10_1101-2021_01_08_425864 554 34 February February NNP 10_1101-2021_01_08_425864 554 35 9 9 CD 10_1101-2021_01_08_425864 554 36 , , , 10_1101-2021_01_08_425864 554 37 2019 2019 CD 10_1101-2021_01_08_425864 554 38 ) ) -RRB- 10_1101-2021_01_08_425864 554 39 . . . 10_1101-2021_01_08_425864 555 1 739 739 CD 10_1101-2021_01_08_425864 555 2 [ [ -LRB- 10_1101-2021_01_08_425864 555 3 24 24 CD 10_1101-2021_01_08_425864 555 4 ] ] -RRB- 10_1101-2021_01_08_425864 555 5 L.B. L.B. NNP 10_1101-2021_01_08_425864 556 1 Willis Willis NNP 10_1101-2021_01_08_425864 556 2 , , , 10_1101-2021_01_08_425864 556 3 W. W. NNP 10_1101-2021_01_08_425864 556 4 Saridah Saridah NNP 10_1101-2021_01_08_425864 556 5 , , , 10_1101-2021_01_08_425864 556 6 W. W. NNP 10_1101-2021_01_08_425864 556 7 Omar Omar NNP 10_1101-2021_01_08_425864 556 8 , , , 10_1101-2021_01_08_425864 556 9 ; ; : 10_1101-2021_01_08_425864 556 10 Ravigadevi Ravigadevi NNP 10_1101-2021_01_08_425864 556 11 Sambanthamurthi Sambanthamurthi NNP 10_1101-2021_01_08_425864 556 12 , , , 10_1101-2021_01_08_425864 556 13 A.J. A.J. NNP 10_1101-2021_01_08_425864 557 1 Sinskey Sinskey NNP 10_1101-2021_01_08_425864 557 2 , , , 10_1101-2021_01_08_425864 557 3 Non- Non- NNP 10_1101-2021_01_08_425864 557 4 740 740 CD 10_1101-2021_01_08_425864 557 5 radioactive radioactive JJ 10_1101-2021_01_08_425864 557 6 assay assay NN 10_1101-2021_01_08_425864 557 7 for for IN 10_1101-2021_01_08_425864 557 8 Acetyl Acetyl NNP 10_1101-2021_01_08_425864 557 9 - - HYPH 10_1101-2021_01_08_425864 557 10 CoA CoA NNP 10_1101-2021_01_08_425864 557 11 carboxylase carboxylase NN 10_1101-2021_01_08_425864 557 12 activity activity NN 10_1101-2021_01_08_425864 557 13 , , , 10_1101-2021_01_08_425864 557 14 2008 2008 CD 10_1101-2021_01_08_425864 557 15 . . . 10_1101-2021_01_08_425864 558 1 741 741 CD 10_1101-2021_01_08_425864 558 2 http://palmoilis.mpob.gov.my/publications/jopr2008sp2-laura.pdf http://palmoilis.mpob.gov.my/publications/jopr2008sp2-laura.pdf NNS 10_1101-2021_01_08_425864 558 3 ( ( -LRB- 10_1101-2021_01_08_425864 558 4 accessed access VBN 10_1101-2021_01_08_425864 558 5 February February NNP 10_1101-2021_01_08_425864 558 6 9 9 CD 10_1101-2021_01_08_425864 558 7 , , , 10_1101-2021_01_08_425864 558 8 742 742 CD 10_1101-2021_01_08_425864 558 9 2019 2019 CD 10_1101-2021_01_08_425864 558 10 ) ) -RRB- 10_1101-2021_01_08_425864 558 11 . . . 10_1101-2021_01_08_425864 559 1 743 743 CD 10_1101-2021_01_08_425864 559 2 [ [ -LRB- 10_1101-2021_01_08_425864 559 3 25 25 CD 10_1101-2021_01_08_425864 559 4 ] ] -RRB- 10_1101-2021_01_08_425864 559 5 G.E. G.E. NNP 10_1101-2021_01_08_425864 560 1 Racagni Racagni NNP 10_1101-2021_01_08_425864 560 2 , , , 10_1101-2021_01_08_425864 560 3 E.E. E.E. NNP 10_1101-2021_01_08_425864 561 1 Machado Machado NNP 10_1101-2021_01_08_425864 561 2 de de FW 10_1101-2021_01_08_425864 561 3 Domenech Domenech NNP 10_1101-2021_01_08_425864 561 4 , , , 10_1101-2021_01_08_425864 561 5 Characterization Characterization NNP 10_1101-2021_01_08_425864 561 6 of of IN 10_1101-2021_01_08_425864 561 7 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 561 8 cruzi cruzi NNP 10_1101-2021_01_08_425864 561 9 744 744 CD 10_1101-2021_01_08_425864 561 10 hexokinase hexokinase NN 10_1101-2021_01_08_425864 561 11 , , , 10_1101-2021_01_08_425864 561 12 Mol Mol NNP 10_1101-2021_01_08_425864 561 13 . . . 10_1101-2021_01_08_425864 562 1 Biochem Biochem NNP 10_1101-2021_01_08_425864 562 2 . . . 10_1101-2021_01_08_425864 563 1 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 563 2 . . . 10_1101-2021_01_08_425864 564 1 9 9 CD 10_1101-2021_01_08_425864 564 2 ( ( -LRB- 10_1101-2021_01_08_425864 564 3 1983 1983 CD 10_1101-2021_01_08_425864 564 4 ) ) -RRB- 10_1101-2021_01_08_425864 564 5 181–188 181–188 CD 10_1101-2021_01_08_425864 564 6 . . . 10_1101-2021_01_08_425864 565 1 https://doi.org/10.1016/0166- https://doi.org/10.1016/0166- NNP 10_1101-2021_01_08_425864 565 2 745 745 CD 10_1101-2021_01_08_425864 565 3 6851(83)90108 6851(83)90108 NNP 10_1101-2021_01_08_425864 565 4 - - HYPH 10_1101-2021_01_08_425864 565 5 1 1 CD 10_1101-2021_01_08_425864 565 6 . . . 10_1101-2021_01_08_425864 566 1 746 746 CD 10_1101-2021_01_08_425864 566 2 [ [ -LRB- 10_1101-2021_01_08_425864 566 3 26 26 CD 10_1101-2021_01_08_425864 566 4 ] ] -RRB- 10_1101-2021_01_08_425864 566 5 M.F. M.F. NNP 10_1101-2021_01_08_425864 567 1 Rütti Rütti NNP 10_1101-2021_01_08_425864 567 2 , , , 10_1101-2021_01_08_425864 567 3 S. S. NNP 10_1101-2021_01_08_425864 567 4 Richard Richard NNP 10_1101-2021_01_08_425864 567 5 , , , 10_1101-2021_01_08_425864 567 6 A. A. NNP 10_1101-2021_01_08_425864 567 7 Penno Penno NNP 10_1101-2021_01_08_425864 567 8 , , , 10_1101-2021_01_08_425864 567 9 A. A. NNP 10_1101-2021_01_08_425864 567 10 von von NNP 10_1101-2021_01_08_425864 567 11 Eckardstein Eckardstein NNP 10_1101-2021_01_08_425864 567 12 , , , 10_1101-2021_01_08_425864 567 13 T. T. NNP 10_1101-2021_01_08_425864 567 14 Hornemann Hornemann NNP 10_1101-2021_01_08_425864 567 15 , , , 10_1101-2021_01_08_425864 567 16 An an DT 10_1101-2021_01_08_425864 567 17 improved improved JJ 10_1101-2021_01_08_425864 567 18 method method NN 10_1101-2021_01_08_425864 567 19 747 747 CD 10_1101-2021_01_08_425864 567 20 to to TO 10_1101-2021_01_08_425864 567 21 determine determine VB 10_1101-2021_01_08_425864 567 22 serine serine JJ 10_1101-2021_01_08_425864 567 23 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 567 24 activity activity NN 10_1101-2021_01_08_425864 567 25 , , , 10_1101-2021_01_08_425864 567 26 J. J. NNP 10_1101-2021_01_08_425864 567 27 Lipid Lipid NNP 10_1101-2021_01_08_425864 567 28 Res Res NNP 10_1101-2021_01_08_425864 567 29 . . . 10_1101-2021_01_08_425864 568 1 50 50 CD 10_1101-2021_01_08_425864 568 2 ( ( -LRB- 10_1101-2021_01_08_425864 568 3 2009 2009 CD 10_1101-2021_01_08_425864 568 4 ) ) -RRB- 10_1101-2021_01_08_425864 568 5 1237–44 1237–44 ADD 10_1101-2021_01_08_425864 568 6 . . . 10_1101-2021_01_08_425864 569 1 748 748 CD 10_1101-2021_01_08_425864 569 2 https://doi.org/10.1194/jlr.D900001-JLR200 https://doi.org/10.1194/jlr.D900001-JLR200 NNP 10_1101-2021_01_08_425864 569 3 . . . 10_1101-2021_01_08_425864 570 1 749 749 CD 10_1101-2021_01_08_425864 570 2 [ [ -LRB- 10_1101-2021_01_08_425864 570 3 27 27 CD 10_1101-2021_01_08_425864 570 4 ] ] -RRB- 10_1101-2021_01_08_425864 570 5 A. a. NN 10_1101-2021_01_08_425864 570 6 Magdaleno Magdaleno NNP 10_1101-2021_01_08_425864 570 7 , , , 10_1101-2021_01_08_425864 570 8 I.Y. I.Y. NNP 10_1101-2021_01_08_425864 571 1 Ahn Ahn NNP 10_1101-2021_01_08_425864 571 2 , , , 10_1101-2021_01_08_425864 571 3 L.S. L.S. NNP 10_1101-2021_01_08_425864 572 1 Paes Paes NNP 10_1101-2021_01_08_425864 572 2 , , , 10_1101-2021_01_08_425864 572 3 A.M. A.M. NNP 10_1101-2021_01_08_425864 572 4 Silber Silber NNP 10_1101-2021_01_08_425864 572 5 , , , 10_1101-2021_01_08_425864 572 6 Actions Actions NNPS 10_1101-2021_01_08_425864 572 7 of of IN 10_1101-2021_01_08_425864 572 8 a a DT 10_1101-2021_01_08_425864 572 9 proline proline JJ 10_1101-2021_01_08_425864 572 10 analogue analogue NN 10_1101-2021_01_08_425864 572 11 , , , 10_1101-2021_01_08_425864 572 12 L- L- NNP 10_1101-2021_01_08_425864 572 13 750 750 CD 10_1101-2021_01_08_425864 572 14 thiazolidine-4-carboxylic thiazolidine-4-carboxylic JJ 10_1101-2021_01_08_425864 572 15 acid acid NN 10_1101-2021_01_08_425864 572 16 ( ( -LRB- 10_1101-2021_01_08_425864 572 17 T4C t4c NN 10_1101-2021_01_08_425864 572 18 ) ) -RRB- 10_1101-2021_01_08_425864 572 19 , , , 10_1101-2021_01_08_425864 572 20 on on IN 10_1101-2021_01_08_425864 572 21 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 572 22 cruzi cruzi NNS 10_1101-2021_01_08_425864 572 23 , , , 10_1101-2021_01_08_425864 572 24 PLoS PLoS NNP 10_1101-2021_01_08_425864 572 25 One one CD 10_1101-2021_01_08_425864 572 26 . . . 10_1101-2021_01_08_425864 573 1 4 4 CD 10_1101-2021_01_08_425864 573 2 ( ( -LRB- 10_1101-2021_01_08_425864 573 3 2009 2009 CD 10_1101-2021_01_08_425864 573 4 ) ) -RRB- 10_1101-2021_01_08_425864 573 5 e4534 e4534 NNP 10_1101-2021_01_08_425864 573 6 . . . 10_1101-2021_01_08_425864 574 1 751 751 CD 10_1101-2021_01_08_425864 574 2 https://doi.org/10.1371/journal.pone.0004534 https://doi.org/10.1371/journal.pone.0004534 NN 10_1101-2021_01_08_425864 574 3 . . . 10_1101-2021_01_08_425864 575 1 752 752 CD 10_1101-2021_01_08_425864 575 2 [ [ -LRB- 10_1101-2021_01_08_425864 575 3 28 28 CD 10_1101-2021_01_08_425864 575 4 ] ] -RRB- 10_1101-2021_01_08_425864 575 5 F.S. F.S. NNP 10_1101-2021_01_08_425864 576 1 Damasceno Damasceno NNP 10_1101-2021_01_08_425864 576 2 , , , 10_1101-2021_01_08_425864 576 3 M.J. M.J. NNP 10_1101-2021_01_08_425864 576 4 Barison Barison NNP 10_1101-2021_01_08_425864 576 5 , , , 10_1101-2021_01_08_425864 576 6 E.M. E.M. NNP 10_1101-2021_01_08_425864 576 7 Pral Pral NNP 10_1101-2021_01_08_425864 576 8 , , , 10_1101-2021_01_08_425864 576 9 L.S. L.S. NNP 10_1101-2021_01_08_425864 577 1 Paes Paes NNP 10_1101-2021_01_08_425864 577 2 , , , 10_1101-2021_01_08_425864 577 3 A.M. A.M. NNP 10_1101-2021_01_08_425864 577 4 Silber Silber NNP 10_1101-2021_01_08_425864 577 5 , , , 10_1101-2021_01_08_425864 577 6 Memantine Memantine NNP 10_1101-2021_01_08_425864 577 7 , , , 10_1101-2021_01_08_425864 577 8 an an DT 10_1101-2021_01_08_425864 577 9 antagonist antagonist NN 10_1101-2021_01_08_425864 577 10 753 753 CD 10_1101-2021_01_08_425864 577 11 of of IN 10_1101-2021_01_08_425864 577 12 the the DT 10_1101-2021_01_08_425864 577 13 NMDA NMDA NNP 10_1101-2021_01_08_425864 577 14 glutamate glutamate NN 10_1101-2021_01_08_425864 577 15 receptor receptor NN 10_1101-2021_01_08_425864 577 16 , , , 10_1101-2021_01_08_425864 577 17 affects affect VBZ 10_1101-2021_01_08_425864 577 18 cell cell NN 10_1101-2021_01_08_425864 577 19 proliferation proliferation NN 10_1101-2021_01_08_425864 577 20 , , , 10_1101-2021_01_08_425864 577 21 differentiation differentiation NN 10_1101-2021_01_08_425864 577 22 and and CC 10_1101-2021_01_08_425864 577 23 the the DT 10_1101-2021_01_08_425864 577 24 754 754 CD 10_1101-2021_01_08_425864 577 25 intracellular intracellular JJ 10_1101-2021_01_08_425864 577 26 cycle cycle NN 10_1101-2021_01_08_425864 577 27 and and CC 10_1101-2021_01_08_425864 577 28 induces induce VBZ 10_1101-2021_01_08_425864 577 29 apoptosis apoptosis NN 10_1101-2021_01_08_425864 577 30 in in IN 10_1101-2021_01_08_425864 577 31 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 577 32 cruzi cruzi NNS 10_1101-2021_01_08_425864 577 33 , , , 10_1101-2021_01_08_425864 577 34 PLoS PLoS NNP 10_1101-2021_01_08_425864 577 35 Negl Negl NNP 10_1101-2021_01_08_425864 577 36 Trop Trop NNP 10_1101-2021_01_08_425864 577 37 Dis Dis NNP 10_1101-2021_01_08_425864 577 38 . . . 10_1101-2021_01_08_425864 578 1 8 8 CD 10_1101-2021_01_08_425864 578 2 755 755 CD 10_1101-2021_01_08_425864 578 3 ( ( -LRB- 10_1101-2021_01_08_425864 578 4 2014 2014 CD 10_1101-2021_01_08_425864 578 5 ) ) -RRB- 10_1101-2021_01_08_425864 578 6 e2717 e2717 NN 10_1101-2021_01_08_425864 578 7 . . . 10_1101-2021_01_08_425864 579 1 https://doi.org/10.1371/journal.pntd.0002717 https://doi.org/10.1371/journal.pntd.0002717 NNP 10_1101-2021_01_08_425864 579 2 . . . 10_1101-2021_01_08_425864 580 1 756 756 CD 10_1101-2021_01_08_425864 580 2 [ [ -LRB- 10_1101-2021_01_08_425864 580 3 29 29 CD 10_1101-2021_01_08_425864 580 4 ] ] -RRB- 10_1101-2021_01_08_425864 580 5 K. K. NNP 10_1101-2021_01_08_425864 580 6 Figarella Figarella NNP 10_1101-2021_01_08_425864 580 7 , , , 10_1101-2021_01_08_425864 580 8 M. M. NNP 10_1101-2021_01_08_425864 580 9 Rawer Rawer NNP 10_1101-2021_01_08_425864 580 10 , , , 10_1101-2021_01_08_425864 580 11 N.L. N.L. NNP 10_1101-2021_01_08_425864 581 1 Uzcategui Uzcategui NNP 10_1101-2021_01_08_425864 581 2 , , , 10_1101-2021_01_08_425864 581 3 B.K. B.K. NNP 10_1101-2021_01_08_425864 582 1 Kubata Kubata NNP 10_1101-2021_01_08_425864 582 2 , , , 10_1101-2021_01_08_425864 582 3 K. K. NNP 10_1101-2021_01_08_425864 582 4 Lauber Lauber NNP 10_1101-2021_01_08_425864 582 5 , , , 10_1101-2021_01_08_425864 582 6 F. F. NNP 10_1101-2021_01_08_425864 582 7 Madeo Madeo NNP 10_1101-2021_01_08_425864 582 8 , , , 10_1101-2021_01_08_425864 582 9 S. S. NNP 10_1101-2021_01_08_425864 582 10 Wesselborg Wesselborg NNP 10_1101-2021_01_08_425864 582 11 , , , 10_1101-2021_01_08_425864 582 12 757 757 CD 10_1101-2021_01_08_425864 582 13 M. M. NNP 10_1101-2021_01_08_425864 582 14 Duszenko Duszenko NNP 10_1101-2021_01_08_425864 582 15 , , , 10_1101-2021_01_08_425864 582 16 Prostaglandin Prostaglandin NNP 10_1101-2021_01_08_425864 582 17 D2 D2 NNP 10_1101-2021_01_08_425864 582 18 induces induce VBZ 10_1101-2021_01_08_425864 582 19 programmed program VBD 10_1101-2021_01_08_425864 582 20 cell cell NN 10_1101-2021_01_08_425864 582 21 death death NN 10_1101-2021_01_08_425864 582 22 in in IN 10_1101-2021_01_08_425864 582 23 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 582 24 brucei brucei NN 10_1101-2021_01_08_425864 582 25 758 758 CD 10_1101-2021_01_08_425864 582 26 bloodstream bloodstream NN 10_1101-2021_01_08_425864 582 27 form form NN 10_1101-2021_01_08_425864 582 28 , , , 10_1101-2021_01_08_425864 582 29 Cell Cell NNP 10_1101-2021_01_08_425864 582 30 Death Death NNP 10_1101-2021_01_08_425864 582 31 Differ Differ NNP 10_1101-2021_01_08_425864 582 32 . . . 10_1101-2021_01_08_425864 583 1 12 12 CD 10_1101-2021_01_08_425864 583 2 ( ( -LRB- 10_1101-2021_01_08_425864 583 3 2005 2005 CD 10_1101-2021_01_08_425864 583 4 ) ) -RRB- 10_1101-2021_01_08_425864 583 5 335–346 335–346 CD 10_1101-2021_01_08_425864 583 6 . . . 10_1101-2021_01_08_425864 584 1 759 759 CD 10_1101-2021_01_08_425864 584 2 https://doi.org/10.1038/sj.cdd.4401564 https://doi.org/10.1038/sj.cdd.4401564 NNP 10_1101-2021_01_08_425864 584 3 . . . 10_1101-2021_01_08_425864 585 1 .CC .CC NFP 10_1101-2021_01_08_425864 585 2 - - : 10_1101-2021_01_08_425864 585 3 BY by IN 10_1101-2021_01_08_425864 585 4 4.0 4.0 CD 10_1101-2021_01_08_425864 585 5 International international JJ 10_1101-2021_01_08_425864 585 6 licensemade licensemade NN 10_1101-2021_01_08_425864 585 7 available available JJ 10_1101-2021_01_08_425864 585 8 under under IN 10_1101-2021_01_08_425864 585 9 a a DT 10_1101-2021_01_08_425864 585 10 ( ( -LRB- 10_1101-2021_01_08_425864 585 11 which which WDT 10_1101-2021_01_08_425864 585 12 was be VBD 10_1101-2021_01_08_425864 585 13 not not RB 10_1101-2021_01_08_425864 585 14 certified certify VBN 10_1101-2021_01_08_425864 585 15 by by IN 10_1101-2021_01_08_425864 585 16 peer peer NN 10_1101-2021_01_08_425864 585 17 review review NN 10_1101-2021_01_08_425864 585 18 ) ) -RRB- 10_1101-2021_01_08_425864 585 19 is be VBZ 10_1101-2021_01_08_425864 585 20 the the DT 10_1101-2021_01_08_425864 585 21 author author NN 10_1101-2021_01_08_425864 585 22 / / SYM 10_1101-2021_01_08_425864 585 23 funder funder NN 10_1101-2021_01_08_425864 585 24 , , , 10_1101-2021_01_08_425864 585 25 who who WP 10_1101-2021_01_08_425864 585 26 has have VBZ 10_1101-2021_01_08_425864 585 27 granted grant VBN 10_1101-2021_01_08_425864 585 28 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 585 29 a a DT 10_1101-2021_01_08_425864 585 30 license license NN 10_1101-2021_01_08_425864 585 31 to to TO 10_1101-2021_01_08_425864 585 32 display display VB 10_1101-2021_01_08_425864 585 33 the the DT 10_1101-2021_01_08_425864 585 34 preprint preprint NN 10_1101-2021_01_08_425864 585 35 in in IN 10_1101-2021_01_08_425864 585 36 perpetuity perpetuity NN 10_1101-2021_01_08_425864 585 37 . . . 10_1101-2021_01_08_425864 586 1 It -PRON- PRP 10_1101-2021_01_08_425864 586 2 is be VBZ 10_1101-2021_01_08_425864 586 3 The the DT 10_1101-2021_01_08_425864 586 4 copyright copyright NN 10_1101-2021_01_08_425864 586 5 holder holder NN 10_1101-2021_01_08_425864 586 6 for for IN 10_1101-2021_01_08_425864 586 7 this this DT 10_1101-2021_01_08_425864 586 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 586 9 version version NN 10_1101-2021_01_08_425864 586 10 posted post VBD 10_1101-2021_01_08_425864 586 11 January January NNP 10_1101-2021_01_08_425864 586 12 8 8 CD 10_1101-2021_01_08_425864 586 13 , , , 10_1101-2021_01_08_425864 586 14 2021 2021 CD 10_1101-2021_01_08_425864 586 15 . . . 10_1101-2021_01_08_425864 586 16 ; ; : 10_1101-2021_01_08_425864 586 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 586 18 : : : 10_1101-2021_01_08_425864 586 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 586 20 preprint preprint NN 10_1101-2021_01_08_425864 586 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 586 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 586 23 760 760 CD 10_1101-2021_01_08_425864 586 24 [ [ -LRB- 10_1101-2021_01_08_425864 586 25 30 30 CD 10_1101-2021_01_08_425864 586 26 ] ] -RRB- 10_1101-2021_01_08_425864 586 27 G.D. G.D. NNP 10_1101-2021_01_08_425864 586 28 Lopaschuk Lopaschuk NNP 10_1101-2021_01_08_425864 586 29 , , , 10_1101-2021_01_08_425864 586 30 S.R. S.R. NNP 10_1101-2021_01_08_425864 587 1 Wall Wall NNP 10_1101-2021_01_08_425864 587 2 , , , 10_1101-2021_01_08_425864 587 3 P.M. P.M. NNP 10_1101-2021_01_08_425864 587 4 Olley Olley NNP 10_1101-2021_01_08_425864 587 5 , , , 10_1101-2021_01_08_425864 587 6 N.J. New Jersey NNP 10_1101-2021_01_08_425864 587 7 Davies Davies NNP 10_1101-2021_01_08_425864 587 8 , , , 10_1101-2021_01_08_425864 587 9 Etomoxir Etomoxir NNPS 10_1101-2021_01_08_425864 587 10 , , , 10_1101-2021_01_08_425864 587 11 a a DT 10_1101-2021_01_08_425864 587 12 carnitine carnitine NN 10_1101-2021_01_08_425864 587 13 761 761 CD 10_1101-2021_01_08_425864 587 14 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 587 15 I -PRON- PRP 10_1101-2021_01_08_425864 587 16 inhibitor inhibitor VBP 10_1101-2021_01_08_425864 587 17 , , , 10_1101-2021_01_08_425864 587 18 protects protect VBZ 10_1101-2021_01_08_425864 587 19 hearts heart NNS 10_1101-2021_01_08_425864 587 20 from from IN 10_1101-2021_01_08_425864 587 21 fatty fatty NN 10_1101-2021_01_08_425864 587 22 acid acid NN 10_1101-2021_01_08_425864 587 23 - - HYPH 10_1101-2021_01_08_425864 587 24 induced induce VBN 10_1101-2021_01_08_425864 587 25 ischemic ischemic JJ 10_1101-2021_01_08_425864 587 26 injury injury NN 10_1101-2021_01_08_425864 587 27 762 762 CD 10_1101-2021_01_08_425864 587 28 independent independent JJ 10_1101-2021_01_08_425864 587 29 of of IN 10_1101-2021_01_08_425864 587 30 changes change NNS 10_1101-2021_01_08_425864 587 31 in in IN 10_1101-2021_01_08_425864 587 32 long long JJ 10_1101-2021_01_08_425864 587 33 chain chain NN 10_1101-2021_01_08_425864 587 34 acylcarnitine acylcarnitine NN 10_1101-2021_01_08_425864 587 35 . . . 10_1101-2021_01_08_425864 588 1 Circ Circ NNP 10_1101-2021_01_08_425864 588 2 . . . 10_1101-2021_01_08_425864 589 1 Res Res NNP 10_1101-2021_01_08_425864 589 2 . . . 10_1101-2021_01_08_425864 590 1 63 63 CD 10_1101-2021_01_08_425864 590 2 ( ( -LRB- 10_1101-2021_01_08_425864 590 3 1988 1988 CD 10_1101-2021_01_08_425864 590 4 ) ) -RRB- 10_1101-2021_01_08_425864 590 5 1036–1043 1036–1043 CD 10_1101-2021_01_08_425864 590 6 . . . 10_1101-2021_01_08_425864 591 1 763 763 CD 10_1101-2021_01_08_425864 591 2 https://doi.org/10.1161/01.RES.63.8.0.2036 https://doi.org/10.1161/01.RES.63.8.0.2036 NNP 10_1101-2021_01_08_425864 591 3 . . . 10_1101-2021_01_08_425864 592 1 764 764 CD 10_1101-2021_01_08_425864 592 2 [ [ -LRB- 10_1101-2021_01_08_425864 592 3 31 31 CD 10_1101-2021_01_08_425864 592 4 ] ] -RRB- 10_1101-2021_01_08_425864 592 5 C.M. C.M. NNP 10_1101-2021_01_08_425864 593 1 Koeller Koeller NNP 10_1101-2021_01_08_425864 593 2 , , , 10_1101-2021_01_08_425864 593 3 N. N. NNP 10_1101-2021_01_08_425864 593 4 Heise Heise NNP 10_1101-2021_01_08_425864 593 5 , , , 10_1101-2021_01_08_425864 593 6 The the DT 10_1101-2021_01_08_425864 593 7 sphingolipid sphingolipid JJ 10_1101-2021_01_08_425864 593 8 biosynthetic biosynthetic JJ 10_1101-2021_01_08_425864 593 9 pathway pathway NN 10_1101-2021_01_08_425864 593 10 is be VBZ 10_1101-2021_01_08_425864 593 11 a a DT 10_1101-2021_01_08_425864 593 12 potential potential JJ 10_1101-2021_01_08_425864 593 13 target target NN 10_1101-2021_01_08_425864 593 14 for for IN 10_1101-2021_01_08_425864 593 15 765 765 CD 10_1101-2021_01_08_425864 593 16 chemotherapy chemotherapy NN 10_1101-2021_01_08_425864 593 17 against against IN 10_1101-2021_01_08_425864 593 18 Chagas Chagas NNP 10_1101-2021_01_08_425864 593 19 disease disease NN 10_1101-2021_01_08_425864 593 20 , , , 10_1101-2021_01_08_425864 593 21 Enzyme Enzyme NNP 10_1101-2021_01_08_425864 593 22 Res Res NNP 10_1101-2021_01_08_425864 593 23 . . . 10_1101-2021_01_08_425864 594 1 2011 2011 CD 10_1101-2021_01_08_425864 594 2 ( ( -LRB- 10_1101-2021_01_08_425864 594 3 2011 2011 CD 10_1101-2021_01_08_425864 594 4 ) ) -RRB- 10_1101-2021_01_08_425864 594 5 1–13 1–13 CD 10_1101-2021_01_08_425864 594 6 . . . 10_1101-2021_01_08_425864 595 1 766 766 CD 10_1101-2021_01_08_425864 595 2 https://doi.org/10.4061/2011/648159 https://doi.org/10.4061/2011/648159 NNP 10_1101-2021_01_08_425864 595 3 . . . 10_1101-2021_01_08_425864 596 1 767 767 CD 10_1101-2021_01_08_425864 596 2 [ [ -LRB- 10_1101-2021_01_08_425864 596 3 32 32 CD 10_1101-2021_01_08_425864 596 4 ] ] -RRB- 10_1101-2021_01_08_425864 596 5 A.S. A.S. NNP 10_1101-2021_01_08_425864 596 6 Divakaruni Divakaruni NNP 10_1101-2021_01_08_425864 596 7 , , , 10_1101-2021_01_08_425864 596 8 W.Y. W.Y. NNP 10_1101-2021_01_08_425864 597 1 Hsieh Hsieh NNP 10_1101-2021_01_08_425864 597 2 , , , 10_1101-2021_01_08_425864 597 3 L. L. NNP 10_1101-2021_01_08_425864 597 4 Minarrieta Minarrieta NNP 10_1101-2021_01_08_425864 597 5 , , , 10_1101-2021_01_08_425864 597 6 T.N. T.N. NNP 10_1101-2021_01_08_425864 598 1 Duong Duong NNP 10_1101-2021_01_08_425864 598 2 , , , 10_1101-2021_01_08_425864 598 3 K.K.O. K.K.O. NNP 10_1101-2021_01_08_425864 599 1 Kim Kim NNP 10_1101-2021_01_08_425864 599 2 , , , 10_1101-2021_01_08_425864 599 3 B.R. B.R. NNP 10_1101-2021_01_08_425864 600 1 Desousa Desousa NNP 10_1101-2021_01_08_425864 600 2 , , , 10_1101-2021_01_08_425864 600 3 A.Y. A.Y. NNP 10_1101-2021_01_08_425864 601 1 768 768 CD 10_1101-2021_01_08_425864 601 2 Andreyev Andreyev NNP 10_1101-2021_01_08_425864 601 3 , , , 10_1101-2021_01_08_425864 601 4 C.E. C.E. NNP 10_1101-2021_01_08_425864 602 1 Bowman Bowman NNP 10_1101-2021_01_08_425864 602 2 , , , 10_1101-2021_01_08_425864 602 3 K. K. NNP 10_1101-2021_01_08_425864 602 4 Caradonna Caradonna NNP 10_1101-2021_01_08_425864 602 5 , , , 10_1101-2021_01_08_425864 602 6 B.P. B.P. NNP 10_1101-2021_01_08_425864 603 1 Dranka Dranka NNP 10_1101-2021_01_08_425864 603 2 , , , 10_1101-2021_01_08_425864 603 3 D.A. D.A. NNP 10_1101-2021_01_08_425864 604 1 Ferrick Ferrick NNP 10_1101-2021_01_08_425864 604 2 , , , 10_1101-2021_01_08_425864 604 3 M. M. NNP 10_1101-2021_01_08_425864 604 4 Liesa Liesa NNP 10_1101-2021_01_08_425864 604 5 , , , 10_1101-2021_01_08_425864 604 6 L. L. NNP 10_1101-2021_01_08_425864 604 7 Stiles Stiles NNP 10_1101-2021_01_08_425864 604 8 , , , 10_1101-2021_01_08_425864 604 9 769 769 CD 10_1101-2021_01_08_425864 604 10 G.W. G.W. NNP 10_1101-2021_01_08_425864 605 1 Rogers Rogers NNP 10_1101-2021_01_08_425864 605 2 , , , 10_1101-2021_01_08_425864 605 3 D. D. NNP 10_1101-2021_01_08_425864 605 4 Braas Braas NNP 10_1101-2021_01_08_425864 605 5 , , , 10_1101-2021_01_08_425864 605 6 T.P. T.P. NNP 10_1101-2021_01_08_425864 606 1 Ciaraldi Ciaraldi NNP 10_1101-2021_01_08_425864 606 2 , , , 10_1101-2021_01_08_425864 606 3 M.J. M.J. NNP 10_1101-2021_01_08_425864 606 4 Wolfgang Wolfgang NNP 10_1101-2021_01_08_425864 606 5 , , , 10_1101-2021_01_08_425864 606 6 T. T. NNP 10_1101-2021_01_08_425864 606 7 Sparwasser Sparwasser NNP 10_1101-2021_01_08_425864 606 8 , , , 10_1101-2021_01_08_425864 606 9 L. L. NNP 10_1101-2021_01_08_425864 606 10 Berod Berod NNP 10_1101-2021_01_08_425864 606 11 , , , 10_1101-2021_01_08_425864 606 12 S.J. S.J. NNP 10_1101-2021_01_08_425864 607 1 770 770 CD 10_1101-2021_01_08_425864 607 2 Bensinger Bensinger NNP 10_1101-2021_01_08_425864 607 3 , , , 10_1101-2021_01_08_425864 607 4 A.N. A.N. NNP 10_1101-2021_01_08_425864 608 1 Murphy Murphy NNP 10_1101-2021_01_08_425864 608 2 , , , 10_1101-2021_01_08_425864 608 3 Etomoxir Etomoxir NNP 10_1101-2021_01_08_425864 608 4 inhibits inhibit VBZ 10_1101-2021_01_08_425864 608 5 macrophage macrophage NN 10_1101-2021_01_08_425864 608 6 polarization polarization NN 10_1101-2021_01_08_425864 608 7 by by IN 10_1101-2021_01_08_425864 608 8 disrupting disrupt VBG 10_1101-2021_01_08_425864 608 9 CoA CoA NNP 10_1101-2021_01_08_425864 608 10 771 771 CD 10_1101-2021_01_08_425864 608 11 homeostasis homeostasis NN 10_1101-2021_01_08_425864 608 12 . . . 10_1101-2021_01_08_425864 608 13 , , , 10_1101-2021_01_08_425864 608 14 Cell Cell NNP 10_1101-2021_01_08_425864 608 15 Metab Metab NNP 10_1101-2021_01_08_425864 608 16 . . . 10_1101-2021_01_08_425864 609 1 28 28 CD 10_1101-2021_01_08_425864 609 2 ( ( -LRB- 10_1101-2021_01_08_425864 609 3 2018 2018 CD 10_1101-2021_01_08_425864 609 4 ) ) -RRB- 10_1101-2021_01_08_425864 609 5 490 490 CD 10_1101-2021_01_08_425864 609 6 - - SYM 10_1101-2021_01_08_425864 609 7 503.e7 503.e7 CD 10_1101-2021_01_08_425864 609 8 . . . 10_1101-2021_01_08_425864 610 1 https://doi.org/10.1016/j.cmet.2018.06.001 https://doi.org/10.1016/j.cmet.2018.06.001 ADD 10_1101-2021_01_08_425864 610 2 . . . 10_1101-2021_01_08_425864 611 1 772 772 CD 10_1101-2021_01_08_425864 611 2 [ [ -LRB- 10_1101-2021_01_08_425864 611 3 33 33 CD 10_1101-2021_01_08_425864 611 4 ] ] -RRB- 10_1101-2021_01_08_425864 611 5 M. M. NNP 10_1101-2021_01_08_425864 611 6 Duszenko Duszenko NNP 10_1101-2021_01_08_425864 611 7 , , , 10_1101-2021_01_08_425864 611 8 K. K. NNP 10_1101-2021_01_08_425864 611 9 Figarella Figarella NNP 10_1101-2021_01_08_425864 611 10 , , , 10_1101-2021_01_08_425864 611 11 E.T. E.T. NNP 10_1101-2021_01_08_425864 612 1 Macleod Macleod NNP 10_1101-2021_01_08_425864 612 2 , , , 10_1101-2021_01_08_425864 612 3 S.C. South Carolina NNP 10_1101-2021_01_08_425864 612 4 Welburn Welburn NNP 10_1101-2021_01_08_425864 612 5 , , , 10_1101-2021_01_08_425864 612 6 Death death NN 10_1101-2021_01_08_425864 612 7 of of IN 10_1101-2021_01_08_425864 612 8 a a DT 10_1101-2021_01_08_425864 612 9 trypanosome trypanosome NN 10_1101-2021_01_08_425864 612 10 : : : 10_1101-2021_01_08_425864 612 11 a a DT 10_1101-2021_01_08_425864 612 12 selfish selfish JJ 10_1101-2021_01_08_425864 612 13 773 773 CD 10_1101-2021_01_08_425864 612 14 altruism altruism NN 10_1101-2021_01_08_425864 612 15 , , , 10_1101-2021_01_08_425864 612 16 Trends Trends NNP 10_1101-2021_01_08_425864 612 17 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 612 18 . . . 10_1101-2021_01_08_425864 613 1 22 22 CD 10_1101-2021_01_08_425864 613 2 ( ( -LRB- 10_1101-2021_01_08_425864 613 3 2006 2006 CD 10_1101-2021_01_08_425864 613 4 ) ) -RRB- 10_1101-2021_01_08_425864 613 5 536–542 536–542 CD 10_1101-2021_01_08_425864 613 6 . . . 10_1101-2021_01_08_425864 614 1 https://doi.org/10.1016/j.pt.2006.08.010 https://doi.org/10.1016/j.pt.2006.08.010 NNP 10_1101-2021_01_08_425864 614 2 . . . 10_1101-2021_01_08_425864 615 1 774 774 CD 10_1101-2021_01_08_425864 615 2 [ [ -LRB- 10_1101-2021_01_08_425864 615 3 34 34 CD 10_1101-2021_01_08_425864 615 4 ] ] -RRB- 10_1101-2021_01_08_425864 615 5 A.C. A.C. NNP 10_1101-2021_01_08_425864 615 6 Mariano Mariano NNP 10_1101-2021_01_08_425864 615 7 , , , 10_1101-2021_01_08_425864 615 8 R. R. NNP 10_1101-2021_01_08_425864 615 9 Santos Santos NNP 10_1101-2021_01_08_425864 615 10 , , , 10_1101-2021_01_08_425864 615 11 M.S. M.S. NNP 10_1101-2021_01_08_425864 616 1 Gonzalez Gonzalez NNP 10_1101-2021_01_08_425864 616 2 , , , 10_1101-2021_01_08_425864 616 3 D. D. NNP 10_1101-2021_01_08_425864 616 4 Feder Feder NNP 10_1101-2021_01_08_425864 616 5 , , , 10_1101-2021_01_08_425864 616 6 E.A. E.A. NNP 10_1101-2021_01_08_425864 617 1 Machado Machado NNP 10_1101-2021_01_08_425864 617 2 , , , 10_1101-2021_01_08_425864 617 3 B. B. NNP 10_1101-2021_01_08_425864 617 4 Pascarelli Pascarelli NNP 10_1101-2021_01_08_425864 617 5 , , , 10_1101-2021_01_08_425864 617 6 K.C. K.C. NNP 10_1101-2021_01_08_425864 618 1 775 775 CD 10_1101-2021_01_08_425864 618 2 Gondim Gondim NNP 10_1101-2021_01_08_425864 618 3 , , , 10_1101-2021_01_08_425864 618 4 J.R. J.R. NNP 10_1101-2021_01_08_425864 618 5 Meyer Meyer NNP 10_1101-2021_01_08_425864 618 6 - - HYPH 10_1101-2021_01_08_425864 618 7 Fernandes Fernandes NNP 10_1101-2021_01_08_425864 618 8 , , , 10_1101-2021_01_08_425864 618 9 Synthesis Synthesis NNP 10_1101-2021_01_08_425864 618 10 and and CC 10_1101-2021_01_08_425864 618 11 mobilization mobilization NN 10_1101-2021_01_08_425864 618 12 of of IN 10_1101-2021_01_08_425864 618 13 glycogen glycogen NNP 10_1101-2021_01_08_425864 618 14 and and CC 10_1101-2021_01_08_425864 618 15 trehalose trehalose DT 10_1101-2021_01_08_425864 618 16 in in IN 10_1101-2021_01_08_425864 618 17 776 776 CD 10_1101-2021_01_08_425864 618 18 adult adult NN 10_1101-2021_01_08_425864 618 19 male male JJ 10_1101-2021_01_08_425864 618 20 Rhodnius Rhodnius NNP 10_1101-2021_01_08_425864 618 21 prolixus prolixus NN 10_1101-2021_01_08_425864 618 22 , , , 10_1101-2021_01_08_425864 618 23 Arch Arch NNP 10_1101-2021_01_08_425864 618 24 . . . 10_1101-2021_01_08_425864 619 1 Insect Insect NNP 10_1101-2021_01_08_425864 619 2 Biochem Biochem NNP 10_1101-2021_01_08_425864 619 3 . . . 10_1101-2021_01_08_425864 620 1 Physiol Physiol NNP 10_1101-2021_01_08_425864 620 2 . . . 10_1101-2021_01_08_425864 621 1 72 72 CD 10_1101-2021_01_08_425864 621 2 ( ( -LRB- 10_1101-2021_01_08_425864 621 3 2009 2009 CD 10_1101-2021_01_08_425864 621 4 ) ) -RRB- 10_1101-2021_01_08_425864 621 5 1–15 1–15 ADD 10_1101-2021_01_08_425864 621 6 . . . 10_1101-2021_01_08_425864 622 1 777 777 CD 10_1101-2021_01_08_425864 622 2 https://doi.org/10.1002/arch.20319 https://doi.org/10.1002/arch.20319 ADD 10_1101-2021_01_08_425864 622 3 . . . 10_1101-2021_01_08_425864 623 1 778 778 CD 10_1101-2021_01_08_425864 623 2 [ [ -LRB- 10_1101-2021_01_08_425864 623 3 35 35 CD 10_1101-2021_01_08_425864 623 4 ] ] -RRB- 10_1101-2021_01_08_425864 623 5 J.M.C. J.M.C. NNP 10_1101-2021_01_08_425864 624 1 Ribeiro Ribeiro NNP 10_1101-2021_01_08_425864 624 2 , , , 10_1101-2021_01_08_425864 624 3 F.A. F.A. NNP 10_1101-2021_01_08_425864 625 1 Genta Genta NNP 10_1101-2021_01_08_425864 625 2 , , , 10_1101-2021_01_08_425864 625 3 M.H.F. M.H.F. NNP 10_1101-2021_01_08_425864 626 1 Sorgine Sorgine NNP 10_1101-2021_01_08_425864 626 2 , , , 10_1101-2021_01_08_425864 626 3 R. R. NNP 10_1101-2021_01_08_425864 626 4 Logullo Logullo NNP 10_1101-2021_01_08_425864 626 5 , , , 10_1101-2021_01_08_425864 626 6 R.D. R.D. NNP 10_1101-2021_01_08_425864 627 1 Mesquita Mesquita NNP 10_1101-2021_01_08_425864 627 2 , , , 10_1101-2021_01_08_425864 627 3 G.O. G.O. NNP 10_1101-2021_01_08_425864 628 1 Paiva Paiva NNP 10_1101-2021_01_08_425864 628 2 - - HYPH 10_1101-2021_01_08_425864 628 3 Silva Silva NNP 10_1101-2021_01_08_425864 628 4 , , , 10_1101-2021_01_08_425864 628 5 779 779 CD 10_1101-2021_01_08_425864 628 6 D. D. NNP 10_1101-2021_01_08_425864 628 7 Majerowicz Majerowicz NNP 10_1101-2021_01_08_425864 628 8 , , , 10_1101-2021_01_08_425864 628 9 M. M. NNP 10_1101-2021_01_08_425864 628 10 Medeiros Medeiros NNP 10_1101-2021_01_08_425864 628 11 , , , 10_1101-2021_01_08_425864 628 12 L. L. NNP 10_1101-2021_01_08_425864 628 13 Koerich Koerich NNP 10_1101-2021_01_08_425864 628 14 , , , 10_1101-2021_01_08_425864 628 15 W.R. W.R. NNP 10_1101-2021_01_08_425864 628 16 Terra Terra NNP 10_1101-2021_01_08_425864 628 17 , , , 10_1101-2021_01_08_425864 628 18 C. C. NNP 10_1101-2021_01_08_425864 628 19 Ferreira Ferreira NNP 10_1101-2021_01_08_425864 628 20 , , , 10_1101-2021_01_08_425864 628 21 A.C. A.C. NNP 10_1101-2021_01_08_425864 628 22 Pimentel Pimentel NNP 10_1101-2021_01_08_425864 628 23 , , , 10_1101-2021_01_08_425864 628 24 P.M. P.M. NNP 10_1101-2021_01_08_425864 628 25 780 780 CD 10_1101-2021_01_08_425864 628 26 Bisch Bisch NNP 10_1101-2021_01_08_425864 628 27 , , , 10_1101-2021_01_08_425864 628 28 D.C. D.C. NNP 10_1101-2021_01_08_425864 628 29 Leite Leite NNP 10_1101-2021_01_08_425864 628 30 , , , 10_1101-2021_01_08_425864 628 31 M.M.P. M.M.P. NNP 10_1101-2021_01_08_425864 629 1 Diniz Diniz NNP 10_1101-2021_01_08_425864 629 2 , , , 10_1101-2021_01_08_425864 629 3 J.L. J.L. NNP 10_1101-2021_01_08_425864 630 1 da da NNP 10_1101-2021_01_08_425864 630 2 S.G. S.G. NNP 10_1101-2021_01_08_425864 630 3 V. V. NNP 10_1101-2021_01_08_425864 630 4 Junior Junior NNP 10_1101-2021_01_08_425864 630 5 , , , 10_1101-2021_01_08_425864 630 6 M.L. M.L. NNP 10_1101-2021_01_08_425864 631 1 Da Da NNP 10_1101-2021_01_08_425864 631 2 Silva Silva NNP 10_1101-2021_01_08_425864 631 3 , , , 10_1101-2021_01_08_425864 631 4 R.N. R.N. NNP 10_1101-2021_01_08_425864 632 1 Araujo Araujo NNP 10_1101-2021_01_08_425864 632 2 , , , 10_1101-2021_01_08_425864 632 3 A.C.P. A.C.P. NNP 10_1101-2021_01_08_425864 633 1 781 781 CD 10_1101-2021_01_08_425864 633 2 Gandara Gandara NNP 10_1101-2021_01_08_425864 633 3 , , , 10_1101-2021_01_08_425864 633 4 S. S. NNP 10_1101-2021_01_08_425864 633 5 Brosson Brosson NNP 10_1101-2021_01_08_425864 633 6 , , , 10_1101-2021_01_08_425864 633 7 D. D. NNP 10_1101-2021_01_08_425864 633 8 Salmon Salmon NNP 10_1101-2021_01_08_425864 633 9 , , , 10_1101-2021_01_08_425864 633 10 S. S. NNP 10_1101-2021_01_08_425864 633 11 Bousbata Bousbata NNP 10_1101-2021_01_08_425864 633 12 , , , 10_1101-2021_01_08_425864 633 13 N. N. NNP 10_1101-2021_01_08_425864 633 14 González González NNP 10_1101-2021_01_08_425864 633 15 - - HYPH 10_1101-2021_01_08_425864 633 16 Caballero Caballero NNP 10_1101-2021_01_08_425864 633 17 , , , 10_1101-2021_01_08_425864 633 18 A.M. A.M. NNP 10_1101-2021_01_08_425864 633 19 Silber Silber NNP 10_1101-2021_01_08_425864 633 20 , , , 10_1101-2021_01_08_425864 633 21 M. M. NNP 10_1101-2021_01_08_425864 633 22 782 782 NNP 10_1101-2021_01_08_425864 633 23 Alves Alves NNP 10_1101-2021_01_08_425864 633 24 - - HYPH 10_1101-2021_01_08_425864 633 25 Bezerra Bezerra NNP 10_1101-2021_01_08_425864 633 26 , , , 10_1101-2021_01_08_425864 633 27 K.C. K.C. NNP 10_1101-2021_01_08_425864 634 1 Gondim Gondim NNP 10_1101-2021_01_08_425864 634 2 , , , 10_1101-2021_01_08_425864 634 3 M.A.C. M.A.C. NNP 10_1101-2021_01_08_425864 635 1 Silva Silva NNP 10_1101-2021_01_08_425864 635 2 - - HYPH 10_1101-2021_01_08_425864 635 3 Neto Neto NNP 10_1101-2021_01_08_425864 635 4 , , , 10_1101-2021_01_08_425864 635 5 G.C. G.C. NNP 10_1101-2021_01_08_425864 636 1 Atella Atella NNP 10_1101-2021_01_08_425864 636 2 , , , 10_1101-2021_01_08_425864 636 3 H. H. NNP 10_1101-2021_01_08_425864 636 4 Araujo Araujo NNP 10_1101-2021_01_08_425864 636 5 , , , 10_1101-2021_01_08_425864 636 6 F.A. F.A. NNP 10_1101-2021_01_08_425864 637 1 Dias Dias NNP 10_1101-2021_01_08_425864 637 2 , , , 10_1101-2021_01_08_425864 637 3 C. C. NNP 10_1101-2021_01_08_425864 637 4 783 783 CD 10_1101-2021_01_08_425864 637 5 Polycarpo Polycarpo NNP 10_1101-2021_01_08_425864 637 6 , , , 10_1101-2021_01_08_425864 637 7 R.J. R.J. NNP 10_1101-2021_01_08_425864 637 8 Vionette Vionette NNP 10_1101-2021_01_08_425864 637 9 - - HYPH 10_1101-2021_01_08_425864 637 10 Amaral Amaral NNP 10_1101-2021_01_08_425864 637 11 , , , 10_1101-2021_01_08_425864 637 12 P. P. NNP 10_1101-2021_01_08_425864 637 13 Fampa Fampa NNP 10_1101-2021_01_08_425864 637 14 , , , 10_1101-2021_01_08_425864 637 15 A.C.A. A.C.A. NNP 10_1101-2021_01_08_425864 638 1 Melo Melo NNP 10_1101-2021_01_08_425864 638 2 , , , 10_1101-2021_01_08_425864 638 3 A.S. A.S. NNP 10_1101-2021_01_08_425864 638 4 Tanaka Tanaka NNP 10_1101-2021_01_08_425864 638 5 , , , 10_1101-2021_01_08_425864 638 6 C. C. NNP 10_1101-2021_01_08_425864 638 7 Balczun Balczun NNP 10_1101-2021_01_08_425864 638 8 , , , 10_1101-2021_01_08_425864 638 9 J.H.M. J.H.M. NNP 10_1101-2021_01_08_425864 639 1 784 784 CD 10_1101-2021_01_08_425864 639 2 Oliveira Oliveira NNP 10_1101-2021_01_08_425864 639 3 , , , 10_1101-2021_01_08_425864 639 4 R.L.S. R.L.S. NNP 10_1101-2021_01_08_425864 640 1 Gonçalves gonçalve NNS 10_1101-2021_01_08_425864 640 2 , , , 10_1101-2021_01_08_425864 640 3 C. C. NNP 10_1101-2021_01_08_425864 640 4 Lazoski Lazoski NNP 10_1101-2021_01_08_425864 640 5 , , , 10_1101-2021_01_08_425864 640 6 R. R. NNP 10_1101-2021_01_08_425864 640 7 Rivera Rivera NNP 10_1101-2021_01_08_425864 640 8 - - HYPH 10_1101-2021_01_08_425864 640 9 Pomar Pomar NNP 10_1101-2021_01_08_425864 640 10 , , , 10_1101-2021_01_08_425864 640 11 L. L. NNP 10_1101-2021_01_08_425864 640 12 Diambra Diambra NNP 10_1101-2021_01_08_425864 640 13 , , , 10_1101-2021_01_08_425864 640 14 G.A. G.A. NNP 10_1101-2021_01_08_425864 641 1 Schaub Schaub NNP 10_1101-2021_01_08_425864 641 2 , , , 10_1101-2021_01_08_425864 641 3 E.S. E.S. NNP 10_1101-2021_01_08_425864 642 1 785 785 CD 10_1101-2021_01_08_425864 642 2 Garcia Garcia NNP 10_1101-2021_01_08_425864 642 3 , , , 10_1101-2021_01_08_425864 642 4 P. P. NNP 10_1101-2021_01_08_425864 642 5 Azambuja Azambuja NNP 10_1101-2021_01_08_425864 642 6 , , , 10_1101-2021_01_08_425864 642 7 G.R.C. G.R.C. NNP 10_1101-2021_01_08_425864 643 1 Braz braz NN 10_1101-2021_01_08_425864 643 2 , , , 10_1101-2021_01_08_425864 643 3 P.L. P.L. NNP 10_1101-2021_01_08_425864 644 1 Oliveira Oliveira NNP 10_1101-2021_01_08_425864 644 2 , , , 10_1101-2021_01_08_425864 644 3 An an DT 10_1101-2021_01_08_425864 644 4 insight insight NN 10_1101-2021_01_08_425864 644 5 into into IN 10_1101-2021_01_08_425864 644 6 the the DT 10_1101-2021_01_08_425864 644 7 transcriptome transcriptome NN 10_1101-2021_01_08_425864 644 8 of of IN 10_1101-2021_01_08_425864 644 9 the the DT 10_1101-2021_01_08_425864 644 10 .CC .CC : 10_1101-2021_01_08_425864 644 11 - - : 10_1101-2021_01_08_425864 644 12 BY by IN 10_1101-2021_01_08_425864 644 13 4.0 4.0 CD 10_1101-2021_01_08_425864 644 14 International international JJ 10_1101-2021_01_08_425864 644 15 licensemade licensemade NN 10_1101-2021_01_08_425864 644 16 available available JJ 10_1101-2021_01_08_425864 644 17 under under IN 10_1101-2021_01_08_425864 644 18 a a DT 10_1101-2021_01_08_425864 644 19 ( ( -LRB- 10_1101-2021_01_08_425864 644 20 which which WDT 10_1101-2021_01_08_425864 644 21 was be VBD 10_1101-2021_01_08_425864 644 22 not not RB 10_1101-2021_01_08_425864 644 23 certified certify VBN 10_1101-2021_01_08_425864 644 24 by by IN 10_1101-2021_01_08_425864 644 25 peer peer NN 10_1101-2021_01_08_425864 644 26 review review NN 10_1101-2021_01_08_425864 644 27 ) ) -RRB- 10_1101-2021_01_08_425864 644 28 is be VBZ 10_1101-2021_01_08_425864 644 29 the the DT 10_1101-2021_01_08_425864 644 30 author author NN 10_1101-2021_01_08_425864 644 31 / / SYM 10_1101-2021_01_08_425864 644 32 funder funder NN 10_1101-2021_01_08_425864 644 33 , , , 10_1101-2021_01_08_425864 644 34 who who WP 10_1101-2021_01_08_425864 644 35 has have VBZ 10_1101-2021_01_08_425864 644 36 granted grant VBN 10_1101-2021_01_08_425864 644 37 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 644 38 a a DT 10_1101-2021_01_08_425864 644 39 license license NN 10_1101-2021_01_08_425864 644 40 to to TO 10_1101-2021_01_08_425864 644 41 display display VB 10_1101-2021_01_08_425864 644 42 the the DT 10_1101-2021_01_08_425864 644 43 preprint preprint NN 10_1101-2021_01_08_425864 644 44 in in IN 10_1101-2021_01_08_425864 644 45 perpetuity perpetuity NN 10_1101-2021_01_08_425864 644 46 . . . 10_1101-2021_01_08_425864 645 1 It -PRON- PRP 10_1101-2021_01_08_425864 645 2 is be VBZ 10_1101-2021_01_08_425864 645 3 The the DT 10_1101-2021_01_08_425864 645 4 copyright copyright NN 10_1101-2021_01_08_425864 645 5 holder holder NN 10_1101-2021_01_08_425864 645 6 for for IN 10_1101-2021_01_08_425864 645 7 this this DT 10_1101-2021_01_08_425864 645 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 645 9 version version NN 10_1101-2021_01_08_425864 645 10 posted post VBD 10_1101-2021_01_08_425864 645 11 January January NNP 10_1101-2021_01_08_425864 645 12 8 8 CD 10_1101-2021_01_08_425864 645 13 , , , 10_1101-2021_01_08_425864 645 14 2021 2021 CD 10_1101-2021_01_08_425864 645 15 . . . 10_1101-2021_01_08_425864 645 16 ; ; : 10_1101-2021_01_08_425864 645 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 645 18 : : : 10_1101-2021_01_08_425864 645 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 645 20 preprint preprint NN 10_1101-2021_01_08_425864 645 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 645 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 645 23 786 786 CD 10_1101-2021_01_08_425864 645 24 digestive digestive JJ 10_1101-2021_01_08_425864 645 25 tract tract NN 10_1101-2021_01_08_425864 645 26 of of IN 10_1101-2021_01_08_425864 645 27 the the DT 10_1101-2021_01_08_425864 645 28 bloodsucking bloodsucking NN 10_1101-2021_01_08_425864 645 29 bug bug NN 10_1101-2021_01_08_425864 645 30 , , , 10_1101-2021_01_08_425864 645 31 Rhodnius Rhodnius NNP 10_1101-2021_01_08_425864 645 32 prolixus prolixus NN 10_1101-2021_01_08_425864 645 33 , , , 10_1101-2021_01_08_425864 645 34 PLoS PLoS NNP 10_1101-2021_01_08_425864 645 35 Negl Negl NNP 10_1101-2021_01_08_425864 645 36 . . . 10_1101-2021_01_08_425864 646 1 Trop Trop NNP 10_1101-2021_01_08_425864 646 2 . . . 10_1101-2021_01_08_425864 647 1 Dis Dis NNP 10_1101-2021_01_08_425864 647 2 . . . 10_1101-2021_01_08_425864 648 1 8 8 CD 10_1101-2021_01_08_425864 648 2 ( ( -LRB- 10_1101-2021_01_08_425864 648 3 2014 2014 CD 10_1101-2021_01_08_425864 648 4 ) ) -RRB- 10_1101-2021_01_08_425864 648 5 787 787 CD 10_1101-2021_01_08_425864 648 6 e2594 e2594 JJ 10_1101-2021_01_08_425864 648 7 . . . 10_1101-2021_01_08_425864 649 1 https://doi.org/10.1371/journal.pntd.0002594 https://doi.org/10.1371/journal.pntd.0002594 NNP 10_1101-2021_01_08_425864 649 2 . . . 10_1101-2021_01_08_425864 650 1 788 788 CD 10_1101-2021_01_08_425864 650 2 [ [ -LRB- 10_1101-2021_01_08_425864 650 3 36 36 CD 10_1101-2021_01_08_425864 650 4 ] ] -RRB- 10_1101-2021_01_08_425864 650 5 L. L. NNP 10_1101-2021_01_08_425864 650 6 Antunes Antunes NNP 10_1101-2021_01_08_425864 650 7 , , , 10_1101-2021_01_08_425864 650 8 J. J. NNP 10_1101-2021_01_08_425864 650 9 Han Han NNP 10_1101-2021_01_08_425864 650 10 , , , 10_1101-2021_01_08_425864 650 11 J. J. NNP 10_1101-2021_01_08_425864 650 12 Pan Pan NNP 10_1101-2021_01_08_425864 650 13 , , , 10_1101-2021_01_08_425864 650 14 C.J.C. C.J.C. NNP 10_1101-2021_01_08_425864 651 1 Moreira Moreira NNP 10_1101-2021_01_08_425864 651 2 , , , 10_1101-2021_01_08_425864 651 3 P. P. NNP 10_1101-2021_01_08_425864 651 4 Azambuja Azambuja NNP 10_1101-2021_01_08_425864 651 5 , , , 10_1101-2021_01_08_425864 651 6 Metabolic metabolic JJ 10_1101-2021_01_08_425864 651 7 signatures signature NNS 10_1101-2021_01_08_425864 651 8 of of IN 10_1101-2021_01_08_425864 651 9 triatomine triatomine JJ 10_1101-2021_01_08_425864 651 10 789 789 CD 10_1101-2021_01_08_425864 651 11 vectors vector NNS 10_1101-2021_01_08_425864 651 12 of of IN 10_1101-2021_01_08_425864 651 13 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 651 14 cruzi cruzi NNS 10_1101-2021_01_08_425864 651 15 unveiled unveil VBN 10_1101-2021_01_08_425864 651 16 by by IN 10_1101-2021_01_08_425864 651 17 metabolomics metabolomic NNS 10_1101-2021_01_08_425864 651 18 , , , 10_1101-2021_01_08_425864 651 19 PLoS PLoS NNP 10_1101-2021_01_08_425864 651 20 One one CD 10_1101-2021_01_08_425864 651 21 . . . 10_1101-2021_01_08_425864 652 1 8 8 CD 10_1101-2021_01_08_425864 652 2 ( ( -LRB- 10_1101-2021_01_08_425864 652 3 2013 2013 CD 10_1101-2021_01_08_425864 652 4 ) ) -RRB- 10_1101-2021_01_08_425864 652 5 77283 77283 CD 10_1101-2021_01_08_425864 652 6 . . . 10_1101-2021_01_08_425864 653 1 790 790 CD 10_1101-2021_01_08_425864 653 2 https://doi.org/10.1371/journal.pone.0077283 https://doi.org/10.1371/journal.pone.0077283 NN 10_1101-2021_01_08_425864 653 3 . . . 10_1101-2021_01_08_425864 654 1 791 791 CD 10_1101-2021_01_08_425864 654 2 [ [ -LRB- 10_1101-2021_01_08_425864 654 3 37 37 CD 10_1101-2021_01_08_425864 654 4 ] ] -RRB- 10_1101-2021_01_08_425864 654 5 K.C. K.C. NNP 10_1101-2021_01_08_425864 655 1 Gondim Gondim NNP 10_1101-2021_01_08_425864 655 2 , , , 10_1101-2021_01_08_425864 655 3 G.C. G.C. NNP 10_1101-2021_01_08_425864 656 1 Atella Atella NNP 10_1101-2021_01_08_425864 656 2 , , , 10_1101-2021_01_08_425864 656 3 E.G. E.G. NNP 10_1101-2021_01_08_425864 657 1 Pontes Pontes NNPS 10_1101-2021_01_08_425864 657 2 , , , 10_1101-2021_01_08_425864 657 3 D. D. NNP 10_1101-2021_01_08_425864 657 4 Majerowicz Majerowicz NNP 10_1101-2021_01_08_425864 657 5 , , , 10_1101-2021_01_08_425864 657 6 Lipid Lipid NNP 10_1101-2021_01_08_425864 657 7 metabolism metabolism NN 10_1101-2021_01_08_425864 657 8 in in IN 10_1101-2021_01_08_425864 657 9 insect insect JJ 10_1101-2021_01_08_425864 657 10 disease disease NN 10_1101-2021_01_08_425864 657 11 792 792 CD 10_1101-2021_01_08_425864 657 12 vectors vector NNS 10_1101-2021_01_08_425864 657 13 , , , 10_1101-2021_01_08_425864 657 14 Insect Insect NNP 10_1101-2021_01_08_425864 657 15 Biochem Biochem NNP 10_1101-2021_01_08_425864 657 16 . . . 10_1101-2021_01_08_425864 658 1 Mol Mol NNP 10_1101-2021_01_08_425864 658 2 . . . 10_1101-2021_01_08_425864 659 1 Biol Biol NNP 10_1101-2021_01_08_425864 659 2 . . . 10_1101-2021_01_08_425864 660 1 101 101 CD 10_1101-2021_01_08_425864 660 2 ( ( -LRB- 10_1101-2021_01_08_425864 660 3 2018 2018 CD 10_1101-2021_01_08_425864 660 4 ) ) -RRB- 10_1101-2021_01_08_425864 660 5 108–123 108–123 CD 10_1101-2021_01_08_425864 660 6 . . . 10_1101-2021_01_08_425864 661 1 793 793 CD 10_1101-2021_01_08_425864 661 2 https://doi.org/10.1016/j.ibmb.2018.08.005 https://doi.org/10.1016/j.ibmb.2018.08.005 CD 10_1101-2021_01_08_425864 661 3 . . . 10_1101-2021_01_08_425864 662 1 794 794 CD 10_1101-2021_01_08_425864 662 2 [ [ -LRB- 10_1101-2021_01_08_425864 662 3 38 38 CD 10_1101-2021_01_08_425864 662 4 ] ] -RRB- 10_1101-2021_01_08_425864 662 5 P.R. P.R. NNP 10_1101-2021_01_08_425864 663 1 Bittencourt Bittencourt NNP 10_1101-2021_01_08_425864 663 2 - - HYPH 10_1101-2021_01_08_425864 663 3 Cunha Cunha NNP 10_1101-2021_01_08_425864 663 4 , , , 10_1101-2021_01_08_425864 663 5 L. L. NNP 10_1101-2021_01_08_425864 663 6 Silva Silva NNP 10_1101-2021_01_08_425864 663 7 - - HYPH 10_1101-2021_01_08_425864 663 8 Cardoso Cardoso NNP 10_1101-2021_01_08_425864 663 9 , , , 10_1101-2021_01_08_425864 663 10 G.A. G.A. NNP 10_1101-2021_01_08_425864 664 1 de de NNP 10_1101-2021_01_08_425864 664 2 Oliveira Oliveira NNP 10_1101-2021_01_08_425864 664 3 , , , 10_1101-2021_01_08_425864 664 4 J.R. J.R. NNP 10_1101-2021_01_08_425864 664 5 da da NNP 10_1101-2021_01_08_425864 664 6 Silva Silva NNP 10_1101-2021_01_08_425864 664 7 , , , 10_1101-2021_01_08_425864 664 8 A.B. A.B. NNP 10_1101-2021_01_08_425864 665 1 da da NNP 10_1101-2021_01_08_425864 665 2 Silveira Silveira NNP 10_1101-2021_01_08_425864 665 3 , , , 10_1101-2021_01_08_425864 665 4 795 795 CD 10_1101-2021_01_08_425864 665 5 G.E.G. G.E.G. NNS 10_1101-2021_01_08_425864 666 1 Kluck Kluck NNP 10_1101-2021_01_08_425864 666 2 , , , 10_1101-2021_01_08_425864 666 3 M. M. NNP 10_1101-2021_01_08_425864 666 4 Souza Souza NNP 10_1101-2021_01_08_425864 666 5 - - HYPH 10_1101-2021_01_08_425864 666 6 Lima Lima NNP 10_1101-2021_01_08_425864 666 7 , , , 10_1101-2021_01_08_425864 666 8 K.C. K.C. NNP 10_1101-2021_01_08_425864 667 1 Gondim Gondim NNP 10_1101-2021_01_08_425864 667 2 , , , 10_1101-2021_01_08_425864 667 3 M. M. NNP 10_1101-2021_01_08_425864 667 4 Dansa Dansa NNP 10_1101-2021_01_08_425864 667 5 - - HYPH 10_1101-2021_01_08_425864 667 6 Petretsky Petretsky NNP 10_1101-2021_01_08_425864 667 7 , , , 10_1101-2021_01_08_425864 667 8 C.P. C.P. NNP 10_1101-2021_01_08_425864 668 1 Silva silva NN 10_1101-2021_01_08_425864 668 2 , , , 10_1101-2021_01_08_425864 668 3 H. H. NNP 10_1101-2021_01_08_425864 668 4 Masuda Masuda NNP 10_1101-2021_01_08_425864 668 5 , , , 10_1101-2021_01_08_425864 668 6 796 796 CD 10_1101-2021_01_08_425864 668 7 M.A.C. M.A.C. NNP 10_1101-2021_01_08_425864 669 1 da da NNP 10_1101-2021_01_08_425864 669 2 Silva Silva NNP 10_1101-2021_01_08_425864 669 3 Neto Neto NNP 10_1101-2021_01_08_425864 669 4 , , , 10_1101-2021_01_08_425864 669 5 G.C. G.C. NNP 10_1101-2021_01_08_425864 670 1 Atella Atella NNP 10_1101-2021_01_08_425864 670 2 , , , 10_1101-2021_01_08_425864 670 3 P.R. P.R. NNP 10_1101-2021_01_08_425864 671 1 Bittencourt Bittencourt NNP 10_1101-2021_01_08_425864 671 2 - - HYPH 10_1101-2021_01_08_425864 671 3 Cunha Cunha NNP 10_1101-2021_01_08_425864 671 4 , , , 10_1101-2021_01_08_425864 671 5 L. L. NNP 10_1101-2021_01_08_425864 671 6 Silva Silva NNP 10_1101-2021_01_08_425864 671 7 - - HYPH 10_1101-2021_01_08_425864 671 8 Cardoso Cardoso NNP 10_1101-2021_01_08_425864 671 9 , , , 10_1101-2021_01_08_425864 671 10 G.A. G.A. NNP 10_1101-2021_01_08_425864 672 1 de de NNP 10_1101-2021_01_08_425864 672 2 797 797 CD 10_1101-2021_01_08_425864 672 3 Oliveira Oliveira NNP 10_1101-2021_01_08_425864 672 4 , , , 10_1101-2021_01_08_425864 672 5 J.R. J.R. NNP 10_1101-2021_01_08_425864 672 6 da da NNP 10_1101-2021_01_08_425864 672 7 Silva Silva NNP 10_1101-2021_01_08_425864 672 8 , , , 10_1101-2021_01_08_425864 672 9 A.B. A.B. NNP 10_1101-2021_01_08_425864 673 1 da da NNP 10_1101-2021_01_08_425864 673 2 Silveira Silveira NNP 10_1101-2021_01_08_425864 673 3 , , , 10_1101-2021_01_08_425864 673 4 G.E.G. G.E.G. NNP 10_1101-2021_01_08_425864 674 1 Kluck Kluck NNP 10_1101-2021_01_08_425864 674 2 , , , 10_1101-2021_01_08_425864 674 3 M. M. NNP 10_1101-2021_01_08_425864 674 4 Souza Souza NNP 10_1101-2021_01_08_425864 674 5 - - HYPH 10_1101-2021_01_08_425864 674 6 Lima Lima NNP 10_1101-2021_01_08_425864 674 7 , , , 10_1101-2021_01_08_425864 674 8 K.C. K.C. NNP 10_1101-2021_01_08_425864 675 1 Gondim Gondim NNP 10_1101-2021_01_08_425864 675 2 , , , 10_1101-2021_01_08_425864 675 3 M. M. NNP 10_1101-2021_01_08_425864 675 4 798 798 CD 10_1101-2021_01_08_425864 675 5 Dansa Dansa NNP 10_1101-2021_01_08_425864 675 6 - - HYPH 10_1101-2021_01_08_425864 675 7 Petretsky Petretsky NNP 10_1101-2021_01_08_425864 675 8 , , , 10_1101-2021_01_08_425864 675 9 C.P. C.P. NNP 10_1101-2021_01_08_425864 676 1 Silva silva NN 10_1101-2021_01_08_425864 676 2 , , , 10_1101-2021_01_08_425864 676 3 H. H. NNP 10_1101-2021_01_08_425864 676 4 Masuda Masuda NNP 10_1101-2021_01_08_425864 676 5 , , , 10_1101-2021_01_08_425864 676 6 M.A.C. M.A.C. NNP 10_1101-2021_01_08_425864 677 1 da da NNP 10_1101-2021_01_08_425864 677 2 Silva Silva NNP 10_1101-2021_01_08_425864 677 3 Neto Neto NNP 10_1101-2021_01_08_425864 677 4 , , , 10_1101-2021_01_08_425864 677 5 G.C. G.C. NNP 10_1101-2021_01_08_425864 678 1 Atella Atella NNP 10_1101-2021_01_08_425864 678 2 , , , 10_1101-2021_01_08_425864 678 3 Perimicrovillar Perimicrovillar NNP 10_1101-2021_01_08_425864 678 4 799 799 CD 10_1101-2021_01_08_425864 678 5 membrane membrane NN 10_1101-2021_01_08_425864 678 6 assembly assembly NN 10_1101-2021_01_08_425864 678 7 : : : 10_1101-2021_01_08_425864 678 8 the the DT 10_1101-2021_01_08_425864 678 9 fate fate NN 10_1101-2021_01_08_425864 678 10 of of IN 10_1101-2021_01_08_425864 678 11 phospholipids phospholipid NNS 10_1101-2021_01_08_425864 678 12 synthesised synthesise VBN 10_1101-2021_01_08_425864 678 13 by by IN 10_1101-2021_01_08_425864 678 14 the the DT 10_1101-2021_01_08_425864 678 15 midgut midgut NN 10_1101-2021_01_08_425864 678 16 of of IN 10_1101-2021_01_08_425864 678 17 Rhodnius Rhodnius NNP 10_1101-2021_01_08_425864 678 18 800 800 CD 10_1101-2021_01_08_425864 678 19 prolixus prolixus NN 10_1101-2021_01_08_425864 678 20 , , , 10_1101-2021_01_08_425864 678 21 Mem Mem NNP 10_1101-2021_01_08_425864 678 22 . . . 10_1101-2021_01_08_425864 679 1 Inst inst RB 10_1101-2021_01_08_425864 679 2 . . . 10_1101-2021_01_08_425864 680 1 Oswaldo Oswaldo NNP 10_1101-2021_01_08_425864 680 2 Cruz Cruz NNP 10_1101-2021_01_08_425864 680 3 . . . 10_1101-2021_01_08_425864 681 1 108 108 CD 10_1101-2021_01_08_425864 681 2 ( ( -LRB- 10_1101-2021_01_08_425864 681 3 2013 2013 CD 10_1101-2021_01_08_425864 681 4 ) ) -RRB- 10_1101-2021_01_08_425864 681 5 494–500 494–500 CD 10_1101-2021_01_08_425864 681 6 . . . 10_1101-2021_01_08_425864 682 1 https://doi.org/10.1590/S0074- https://doi.org/10.1590/s0074- UH 10_1101-2021_01_08_425864 682 2 801 801 CD 10_1101-2021_01_08_425864 682 3 0276108042013016 0276108042013016 CD 10_1101-2021_01_08_425864 682 4 . . . 10_1101-2021_01_08_425864 683 1 802 802 CD 10_1101-2021_01_08_425864 683 2 [ [ -LRB- 10_1101-2021_01_08_425864 683 3 39 39 CD 10_1101-2021_01_08_425864 683 4 ] ] -RRB- 10_1101-2021_01_08_425864 683 5 D.E. D.E. NNP 10_1101-2021_01_08_425864 684 1 Wood wood NN 10_1101-2021_01_08_425864 684 2 , , , 10_1101-2021_01_08_425864 684 3 E.L. E.L. NNP 10_1101-2021_01_08_425864 684 4 Schiller Schiller NNP 10_1101-2021_01_08_425864 684 5 , , , 10_1101-2021_01_08_425864 684 6 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 684 7 cruzi cruzi NNS 10_1101-2021_01_08_425864 684 8 : : : 10_1101-2021_01_08_425864 684 9 comparative comparative JJ 10_1101-2021_01_08_425864 684 10 fatty fatty NN 10_1101-2021_01_08_425864 684 11 acid acid NN 10_1101-2021_01_08_425864 684 12 metabolism metabolism NN 10_1101-2021_01_08_425864 684 13 of of IN 10_1101-2021_01_08_425864 684 14 the the DT 10_1101-2021_01_08_425864 684 15 803 803 CD 10_1101-2021_01_08_425864 684 16 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 684 17 and and CC 10_1101-2021_01_08_425864 684 18 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 684 19 in in IN 10_1101-2021_01_08_425864 684 20 vitro vitro FW 10_1101-2021_01_08_425864 684 21 . . . 10_1101-2021_01_08_425864 685 1 Exp Exp NNP 10_1101-2021_01_08_425864 685 2 . . . 10_1101-2021_01_08_425864 686 1 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 686 2 . . . 10_1101-2021_01_08_425864 687 1 38 38 CD 10_1101-2021_01_08_425864 687 2 ( ( -LRB- 10_1101-2021_01_08_425864 687 3 1975 1975 CD 10_1101-2021_01_08_425864 687 4 ) ) -RRB- 10_1101-2021_01_08_425864 687 5 202–7 202–7 CD 10_1101-2021_01_08_425864 687 6 . . . 10_1101-2021_01_08_425864 688 1 804 804 CD 10_1101-2021_01_08_425864 688 2 http://www.ncbi.nlm.nih.gov/pubmed/1100424 http://www.ncbi.nlm.nih.gov/pubmed/1100424 NN 10_1101-2021_01_08_425864 688 3 . . . 10_1101-2021_01_08_425864 689 1 805 805 CD 10_1101-2021_01_08_425864 689 2 [ [ -LRB- 10_1101-2021_01_08_425864 689 3 40 40 CD 10_1101-2021_01_08_425864 689 4 ] ] -RRB- 10_1101-2021_01_08_425864 689 5 D.E. D.E. NNP 10_1101-2021_01_08_425864 690 1 Wood wood NN 10_1101-2021_01_08_425864 690 2 , , , 10_1101-2021_01_08_425864 690 3 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 690 4 cruzi cruzi NNS 10_1101-2021_01_08_425864 690 5 : : : 10_1101-2021_01_08_425864 690 6 fatty fatty NNP 10_1101-2021_01_08_425864 690 7 acid acid NNP 10_1101-2021_01_08_425864 690 8 metabolism metabolism NN 10_1101-2021_01_08_425864 690 9 in in IN 10_1101-2021_01_08_425864 690 10 vitro vitro FW 10_1101-2021_01_08_425864 690 11 , , , 10_1101-2021_01_08_425864 690 12 Exp Exp NNP 10_1101-2021_01_08_425864 690 13 . . . 10_1101-2021_01_08_425864 691 1 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 691 2 . . . 10_1101-2021_01_08_425864 692 1 37 37 CD 10_1101-2021_01_08_425864 692 2 ( ( -LRB- 10_1101-2021_01_08_425864 692 3 1975 1975 CD 10_1101-2021_01_08_425864 692 4 ) ) -RRB- 10_1101-2021_01_08_425864 692 5 60 60 CD 10_1101-2021_01_08_425864 692 6 – – : 10_1101-2021_01_08_425864 692 7 806 806 CD 10_1101-2021_01_08_425864 692 8 6 6 CD 10_1101-2021_01_08_425864 692 9 . . . 10_1101-2021_01_08_425864 692 10 http://www.ncbi.nlm.nih.gov/pubmed/1090440 http://www.ncbi.nlm.nih.gov/pubmed/1090440 NNS 10_1101-2021_01_08_425864 692 11 . . . 10_1101-2021_01_08_425864 693 1 807 807 CD 10_1101-2021_01_08_425864 693 2 [ [ -LRB- 10_1101-2021_01_08_425864 693 3 41 41 CD 10_1101-2021_01_08_425864 693 4 ] ] -RRB- 10_1101-2021_01_08_425864 693 5 J.J. J.J. NNP 10_1101-2021_01_08_425864 693 6 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 693 7 , , , 10_1101-2021_01_08_425864 693 8 Aerobic aerobic JJ 10_1101-2021_01_08_425864 693 9 fermentation fermentation NN 10_1101-2021_01_08_425864 693 10 of of IN 10_1101-2021_01_08_425864 693 11 glucose glucose NN 10_1101-2021_01_08_425864 693 12 by by IN 10_1101-2021_01_08_425864 693 13 trypanosomatids trypanosomatid NNS 10_1101-2021_01_08_425864 693 14 , , , 10_1101-2021_01_08_425864 693 15 FASEB FASEB NNP 10_1101-2021_01_08_425864 693 16 J. J. NNP 10_1101-2021_01_08_425864 694 1 6 6 CD 10_1101-2021_01_08_425864 694 2 ( ( -LRB- 10_1101-2021_01_08_425864 694 3 1992 1992 CD 10_1101-2021_01_08_425864 694 4 ) ) -RRB- 10_1101-2021_01_08_425864 694 5 3153 3153 CD 10_1101-2021_01_08_425864 694 6 – – : 10_1101-2021_01_08_425864 694 7 808 808 CD 10_1101-2021_01_08_425864 694 8 61 61 CD 10_1101-2021_01_08_425864 694 9 . . . 10_1101-2021_01_08_425864 694 10 https://doi.org/10.1096/FASEBJ.8.0.23.1397837 https://doi.org/10.1096/fasebj.8.0.23.1397837 ADD 10_1101-2021_01_08_425864 694 11 . . . 10_1101-2021_01_08_425864 695 1 809 809 CD 10_1101-2021_01_08_425864 695 2 [ [ -LRB- 10_1101-2021_01_08_425864 695 3 42 42 CD 10_1101-2021_01_08_425864 695 4 ] ] -RRB- 10_1101-2021_01_08_425864 695 5 J.J. J.J. NNP 10_1101-2021_01_08_425864 695 6 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 695 7 , , , 10_1101-2021_01_08_425864 695 8 Intermediate Intermediate NNP 10_1101-2021_01_08_425864 695 9 metabolism metabolism NN 10_1101-2021_01_08_425864 695 10 in in IN 10_1101-2021_01_08_425864 695 11 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 695 12 cruzi cruzi NNS 10_1101-2021_01_08_425864 695 13 , , , 10_1101-2021_01_08_425864 695 14 J. J. NNP 10_1101-2021_01_08_425864 695 15 Bioenerg Bioenerg NNP 10_1101-2021_01_08_425864 695 16 . . . 10_1101-2021_01_08_425864 696 1 Biomembr Biomembr NNP 10_1101-2021_01_08_425864 696 2 . . . 10_1101-2021_01_08_425864 697 1 26 26 CD 10_1101-2021_01_08_425864 697 2 810 810 CD 10_1101-2021_01_08_425864 697 3 ( ( -LRB- 10_1101-2021_01_08_425864 697 4 1994 1994 CD 10_1101-2021_01_08_425864 697 5 ) ) -RRB- 10_1101-2021_01_08_425864 697 6 157–65 157–65 CD 10_1101-2021_01_08_425864 697 7 . . . 10_1101-2021_01_08_425864 698 1 http://www.ncbi.nlm.nih.gov/pubmed/8056782 http://www.ncbi.nlm.nih.gov/pubmed/8056782 NN 10_1101-2021_01_08_425864 698 2 ( ( -LRB- 10_1101-2021_01_08_425864 698 3 accessed access VBN 10_1101-2021_01_08_425864 698 4 June June NNP 10_1101-2021_01_08_425864 698 5 6 6 CD 10_1101-2021_01_08_425864 698 6 , , , 10_1101-2021_01_08_425864 698 7 2019 2019 CD 10_1101-2021_01_08_425864 698 8 ) ) -RRB- 10_1101-2021_01_08_425864 698 9 . . . 10_1101-2021_01_08_425864 699 1 811 811 CD 10_1101-2021_01_08_425864 699 2 [ [ -LRB- 10_1101-2021_01_08_425864 699 3 43 43 CD 10_1101-2021_01_08_425864 699 4 ] ] -RRB- 10_1101-2021_01_08_425864 699 5 B. B. NNP 10_1101-2021_01_08_425864 699 6 Frydman Frydman NNP 10_1101-2021_01_08_425864 699 7 , , , 10_1101-2021_01_08_425864 699 8 C. C. NNP 10_1101-2021_01_08_425864 699 9 Santos Santos NNP 10_1101-2021_01_08_425864 699 10 , , , 10_1101-2021_01_08_425864 699 11 J.J.B. J.J.B. NNP 10_1101-2021_01_08_425864 700 1 Cannata Cannata NNP 10_1101-2021_01_08_425864 700 2 , , , 10_1101-2021_01_08_425864 700 3 J.J. J.J. NNP 10_1101-2021_01_08_425864 700 4 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 700 5 , , , 10_1101-2021_01_08_425864 700 6 Carbon-13 Carbon-13 NNP 10_1101-2021_01_08_425864 700 7 nuclear nuclear JJ 10_1101-2021_01_08_425864 700 8 magnetic magnetic JJ 10_1101-2021_01_08_425864 700 9 resonance resonance NN 10_1101-2021_01_08_425864 700 10 .CC .CC NFP 10_1101-2021_01_08_425864 700 11 - - : 10_1101-2021_01_08_425864 700 12 BY by IN 10_1101-2021_01_08_425864 700 13 4.0 4.0 CD 10_1101-2021_01_08_425864 700 14 International international JJ 10_1101-2021_01_08_425864 700 15 licensemade licensemade NN 10_1101-2021_01_08_425864 700 16 available available JJ 10_1101-2021_01_08_425864 700 17 under under IN 10_1101-2021_01_08_425864 700 18 a a DT 10_1101-2021_01_08_425864 700 19 ( ( -LRB- 10_1101-2021_01_08_425864 700 20 which which WDT 10_1101-2021_01_08_425864 700 21 was be VBD 10_1101-2021_01_08_425864 700 22 not not RB 10_1101-2021_01_08_425864 700 23 certified certify VBN 10_1101-2021_01_08_425864 700 24 by by IN 10_1101-2021_01_08_425864 700 25 peer peer NN 10_1101-2021_01_08_425864 700 26 review review NN 10_1101-2021_01_08_425864 700 27 ) ) -RRB- 10_1101-2021_01_08_425864 700 28 is be VBZ 10_1101-2021_01_08_425864 700 29 the the DT 10_1101-2021_01_08_425864 700 30 author author NN 10_1101-2021_01_08_425864 700 31 / / SYM 10_1101-2021_01_08_425864 700 32 funder funder NN 10_1101-2021_01_08_425864 700 33 , , , 10_1101-2021_01_08_425864 700 34 who who WP 10_1101-2021_01_08_425864 700 35 has have VBZ 10_1101-2021_01_08_425864 700 36 granted grant VBN 10_1101-2021_01_08_425864 700 37 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 700 38 a a DT 10_1101-2021_01_08_425864 700 39 license license NN 10_1101-2021_01_08_425864 700 40 to to TO 10_1101-2021_01_08_425864 700 41 display display VB 10_1101-2021_01_08_425864 700 42 the the DT 10_1101-2021_01_08_425864 700 43 preprint preprint NN 10_1101-2021_01_08_425864 700 44 in in IN 10_1101-2021_01_08_425864 700 45 perpetuity perpetuity NN 10_1101-2021_01_08_425864 700 46 . . . 10_1101-2021_01_08_425864 701 1 It -PRON- PRP 10_1101-2021_01_08_425864 701 2 is be VBZ 10_1101-2021_01_08_425864 701 3 The the DT 10_1101-2021_01_08_425864 701 4 copyright copyright NN 10_1101-2021_01_08_425864 701 5 holder holder NN 10_1101-2021_01_08_425864 701 6 for for IN 10_1101-2021_01_08_425864 701 7 this this DT 10_1101-2021_01_08_425864 701 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 701 9 version version NN 10_1101-2021_01_08_425864 701 10 posted post VBD 10_1101-2021_01_08_425864 701 11 January January NNP 10_1101-2021_01_08_425864 701 12 8 8 CD 10_1101-2021_01_08_425864 701 13 , , , 10_1101-2021_01_08_425864 701 14 2021 2021 CD 10_1101-2021_01_08_425864 701 15 . . . 10_1101-2021_01_08_425864 701 16 ; ; : 10_1101-2021_01_08_425864 701 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 701 18 : : : 10_1101-2021_01_08_425864 701 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 701 20 preprint preprint NN 10_1101-2021_01_08_425864 701 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 701 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 701 23 812 812 CD 10_1101-2021_01_08_425864 701 24 analysis analysis NN 10_1101-2021_01_08_425864 701 25 of of IN 10_1101-2021_01_08_425864 701 26 [ [ -LRB- 10_1101-2021_01_08_425864 701 27 1 1 CD 10_1101-2021_01_08_425864 701 28 - - SYM 10_1101-2021_01_08_425864 701 29 13C]-glucose 13c]-glucose CD 10_1101-2021_01_08_425864 701 30 metabolism metabolism NN 10_1101-2021_01_08_425864 701 31 in in IN 10_1101-2021_01_08_425864 701 32 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 701 33 cruzi cruzi NNS 10_1101-2021_01_08_425864 701 34 . . . 10_1101-2021_01_08_425864 702 1 Evidence evidence NN 10_1101-2021_01_08_425864 702 2 of of IN 10_1101-2021_01_08_425864 702 3 the the DT 10_1101-2021_01_08_425864 702 4 presence presence NN 10_1101-2021_01_08_425864 702 5 of of IN 10_1101-2021_01_08_425864 702 6 813 813 CD 10_1101-2021_01_08_425864 702 7 two two CD 10_1101-2021_01_08_425864 702 8 alanine alanine NN 10_1101-2021_01_08_425864 702 9 pools pool NNS 10_1101-2021_01_08_425864 702 10 and and CC 10_1101-2021_01_08_425864 702 11 of of IN 10_1101-2021_01_08_425864 702 12 two two CD 10_1101-2021_01_08_425864 702 13 CO2 CO2 NNP 10_1101-2021_01_08_425864 702 14 fixation fixation NN 10_1101-2021_01_08_425864 702 15 reactions reaction NNS 10_1101-2021_01_08_425864 702 16 , , , 10_1101-2021_01_08_425864 702 17 Eur Eur NNP 10_1101-2021_01_08_425864 702 18 . . . 10_1101-2021_01_08_425864 703 1 J. J. NNP 10_1101-2021_01_08_425864 703 2 Biochem Biochem NNP 10_1101-2021_01_08_425864 703 3 . . . 10_1101-2021_01_08_425864 704 1 192 192 CD 10_1101-2021_01_08_425864 704 2 ( ( -LRB- 10_1101-2021_01_08_425864 704 3 1990 1990 CD 10_1101-2021_01_08_425864 704 4 ) ) -RRB- 10_1101-2021_01_08_425864 704 5 363–368 363–368 CD 10_1101-2021_01_08_425864 704 6 . . . 10_1101-2021_01_08_425864 705 1 814 814 CD 10_1101-2021_01_08_425864 705 2 https://doi.org/10.1111/j.1432-1033.1990.tb19235.x https://doi.org/10.1111/j.1432-1033.1990.tb19235.x NNP 10_1101-2021_01_08_425864 705 3 . . . 10_1101-2021_01_08_425864 706 1 815 815 CD 10_1101-2021_01_08_425864 706 2 [ [ -LRB- 10_1101-2021_01_08_425864 706 3 44 44 CD 10_1101-2021_01_08_425864 706 4 ] ] -RRB- 10_1101-2021_01_08_425864 706 5 A.E. A.E. NNP 10_1101-2021_01_08_425864 707 1 Leroux Leroux NNP 10_1101-2021_01_08_425864 707 2 , , , 10_1101-2021_01_08_425864 707 3 D.A. D.A. NNP 10_1101-2021_01_08_425864 708 1 Maugeri Maugeri NNP 10_1101-2021_01_08_425864 708 2 , , , 10_1101-2021_01_08_425864 708 3 F.R. F.R. NNP 10_1101-2021_01_08_425864 709 1 Opperdoes opperdoe NNS 10_1101-2021_01_08_425864 709 2 , , , 10_1101-2021_01_08_425864 709 3 J.J. J.J. NNP 10_1101-2021_01_08_425864 709 4 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 709 5 , , , 10_1101-2021_01_08_425864 709 6 C. C. NNP 10_1101-2021_01_08_425864 709 7 Nowicki Nowicki NNP 10_1101-2021_01_08_425864 709 8 , , , 10_1101-2021_01_08_425864 709 9 Comparative comparative JJ 10_1101-2021_01_08_425864 709 10 studies study NNS 10_1101-2021_01_08_425864 709 11 816 816 CD 10_1101-2021_01_08_425864 709 12 on on IN 10_1101-2021_01_08_425864 709 13 the the DT 10_1101-2021_01_08_425864 709 14 biochemical biochemical JJ 10_1101-2021_01_08_425864 709 15 properties property NNS 10_1101-2021_01_08_425864 709 16 of of IN 10_1101-2021_01_08_425864 709 17 the the DT 10_1101-2021_01_08_425864 709 18 malic malic JJ 10_1101-2021_01_08_425864 709 19 enzymes enzyme NNS 10_1101-2021_01_08_425864 709 20 from from IN 10_1101-2021_01_08_425864 709 21 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 709 22 cruzi cruzi NNS 10_1101-2021_01_08_425864 709 23     _SP 10_1101-2021_01_08_425864 709 24 and and CC 10_1101-2021_01_08_425864 709 25 817 817 CD 10_1101-2021_01_08_425864 709 26 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 709 27 brucei brucei NN 10_1101-2021_01_08_425864 709 28 , , , 10_1101-2021_01_08_425864 709 29 FEMS FEMS NNP 10_1101-2021_01_08_425864 709 30 Microbiol Microbiol NNP 10_1101-2021_01_08_425864 709 31 . . . 10_1101-2021_01_08_425864 710 1 Lett Lett NNP 10_1101-2021_01_08_425864 710 2 . . . 10_1101-2021_01_08_425864 711 1 314 314 CD 10_1101-2021_01_08_425864 711 2 ( ( -LRB- 10_1101-2021_01_08_425864 711 3 2011 2011 CD 10_1101-2021_01_08_425864 711 4 ) ) -RRB- 10_1101-2021_01_08_425864 711 5 25–33 25–33 CD 10_1101-2021_01_08_425864 711 6 . . . 10_1101-2021_01_08_425864 712 1 818 818 CD 10_1101-2021_01_08_425864 712 2 https://doi.org/10.1111/j.1574-6968.2010.02142.x https://doi.org/10.1111/j.1574-6968.2010.02142.x NN 10_1101-2021_01_08_425864 712 3 . . . 10_1101-2021_01_08_425864 713 1 819 819 CD 10_1101-2021_01_08_425864 713 2 [ [ -LRB- 10_1101-2021_01_08_425864 713 3 45 45 CD 10_1101-2021_01_08_425864 713 4 ] ] -RRB- 10_1101-2021_01_08_425864 713 5 C. C. NNP 10_1101-2021_01_08_425864 713 6 Zelada Zelada NNP 10_1101-2021_01_08_425864 713 7 , , , 10_1101-2021_01_08_425864 713 8 M. M. NNP 10_1101-2021_01_08_425864 713 9 Montemartini Montemartini NNP 10_1101-2021_01_08_425864 713 10 , , , 10_1101-2021_01_08_425864 713 11 J.J. J.J. NNP 10_1101-2021_01_08_425864 713 12 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 713 13 , , , 10_1101-2021_01_08_425864 713 14 C. C. NNP 10_1101-2021_01_08_425864 713 15 Nowicki Nowicki NNP 10_1101-2021_01_08_425864 713 16 , , , 10_1101-2021_01_08_425864 713 17 Purification Purification NNP 10_1101-2021_01_08_425864 713 18 and and CC 10_1101-2021_01_08_425864 713 19 partial partial JJ 10_1101-2021_01_08_425864 713 20 structural structural NN 10_1101-2021_01_08_425864 713 21 and and CC 10_1101-2021_01_08_425864 713 22 820 820 CD 10_1101-2021_01_08_425864 713 23 kinetic kinetic JJ 10_1101-2021_01_08_425864 713 24 characterization characterization NN 10_1101-2021_01_08_425864 713 25 of of IN 10_1101-2021_01_08_425864 713 26 an an DT 10_1101-2021_01_08_425864 713 27 alanine alanine NN 10_1101-2021_01_08_425864 713 28 aminotransferase aminotransferase NN 10_1101-2021_01_08_425864 713 29 from from IN 10_1101-2021_01_08_425864 713 30 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 713 31 of of IN 10_1101-2021_01_08_425864 713 32 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 713 33 821 821 CD 10_1101-2021_01_08_425864 713 34 cruzi cruzi NNS 10_1101-2021_01_08_425864 713 35 , , , 10_1101-2021_01_08_425864 713 36 Mol Mol NNP 10_1101-2021_01_08_425864 713 37 . . . 10_1101-2021_01_08_425864 714 1 Biochem Biochem NNP 10_1101-2021_01_08_425864 714 2 . . . 10_1101-2021_01_08_425864 715 1 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 715 2 . . . 10_1101-2021_01_08_425864 716 1 79 79 CD 10_1101-2021_01_08_425864 716 2 ( ( -LRB- 10_1101-2021_01_08_425864 716 3 1996 1996 CD 10_1101-2021_01_08_425864 716 4 ) ) -RRB- 10_1101-2021_01_08_425864 716 5 225–228 225–228 CD 10_1101-2021_01_08_425864 716 6 . . . 10_1101-2021_01_08_425864 717 1 https://doi.org/10.1016/0166- https://doi.org/10.1016/0166- NNP 10_1101-2021_01_08_425864 717 2 822 822 CD 10_1101-2021_01_08_425864 717 3 6851(96)02652 6851(96)02652 CD 10_1101-2021_01_08_425864 717 4 - - HYPH 10_1101-2021_01_08_425864 717 5 7 7 CD 10_1101-2021_01_08_425864 717 6 . . . 10_1101-2021_01_08_425864 718 1 823 823 CD 10_1101-2021_01_08_425864 718 2 [ [ -LRB- 10_1101-2021_01_08_425864 718 3 46 46 CD 10_1101-2021_01_08_425864 718 4 ] ] -RRB- 10_1101-2021_01_08_425864 718 5 M. M. NNP 10_1101-2021_01_08_425864 718 6 Montemartini Montemartini NNP 10_1101-2021_01_08_425864 718 7 , , , 10_1101-2021_01_08_425864 718 8 J. J. NNP 10_1101-2021_01_08_425864 718 9 Buá Buá NNP 10_1101-2021_01_08_425864 718 10 , , , 10_1101-2021_01_08_425864 718 11 E. E. NNP 10_1101-2021_01_08_425864 718 12 Bontempi Bontempi NNP 10_1101-2021_01_08_425864 718 13 , , , 10_1101-2021_01_08_425864 718 14 C. C. NNP 10_1101-2021_01_08_425864 718 15 Zelada Zelada NNP 10_1101-2021_01_08_425864 718 16 , , , 10_1101-2021_01_08_425864 718 17 A.M. A.M. NNP 10_1101-2021_01_08_425864 718 18 Ruiz Ruiz NNP 10_1101-2021_01_08_425864 718 19 , , , 10_1101-2021_01_08_425864 718 20 J. J. NNP 10_1101-2021_01_08_425864 718 21 Santomé Santomé NNP 10_1101-2021_01_08_425864 718 22 , , , 10_1101-2021_01_08_425864 718 23 J. J. NNP 10_1101-2021_01_08_425864 718 24 José José NNP 10_1101-2021_01_08_425864 718 25 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 718 26 , , , 10_1101-2021_01_08_425864 718 27 824 824 CD 10_1101-2021_01_08_425864 718 28 C. C. NNP 10_1101-2021_01_08_425864 718 29 Nowicki Nowicki NNP 10_1101-2021_01_08_425864 718 30 , , , 10_1101-2021_01_08_425864 718 31 A a DT 10_1101-2021_01_08_425864 718 32 recombinant recombinant JJ 10_1101-2021_01_08_425864 718 33 tyrosine tyrosine JJ 10_1101-2021_01_08_425864 718 34 aminotransferase aminotransferase NN 10_1101-2021_01_08_425864 718 35 from from IN 10_1101-2021_01_08_425864 718 36 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 718 37 cruzi cruzi NNS 10_1101-2021_01_08_425864 718 38 has have VBZ 10_1101-2021_01_08_425864 718 39 both both CC 10_1101-2021_01_08_425864 718 40 825 825 CD 10_1101-2021_01_08_425864 718 41 tyrosine tyrosine NN 10_1101-2021_01_08_425864 718 42 aminotransferase aminotransferase NN 10_1101-2021_01_08_425864 718 43 and and CC 10_1101-2021_01_08_425864 718 44 alanine alanine NN 10_1101-2021_01_08_425864 718 45 aminotransferase aminotransferase NN 10_1101-2021_01_08_425864 718 46 activities activity NNS 10_1101-2021_01_08_425864 718 47 , , , 10_1101-2021_01_08_425864 718 48 FEMS FEMS NNP 10_1101-2021_01_08_425864 718 49 Microbiol Microbiol NNP 10_1101-2021_01_08_425864 718 50 . . . 10_1101-2021_01_08_425864 719 1 Lett Lett NNP 10_1101-2021_01_08_425864 719 2 . . . 10_1101-2021_01_08_425864 720 1 826 826 CD 10_1101-2021_01_08_425864 720 2 133 133 CD 10_1101-2021_01_08_425864 720 3 ( ( -LRB- 10_1101-2021_01_08_425864 720 4 1995 1995 CD 10_1101-2021_01_08_425864 720 5 ) ) -RRB- 10_1101-2021_01_08_425864 720 6 17–20 17–20 CD 10_1101-2021_01_08_425864 720 7 . . . 10_1101-2021_01_08_425864 721 1 https://doi.org/10.1111/j.1574-6968.1995.tb07854.x https://doi.org/10.1111/j.1574-6968.1995.tb07854.x NNP 10_1101-2021_01_08_425864 721 2 . . . 10_1101-2021_01_08_425864 722 1 827 827 CD 10_1101-2021_01_08_425864 722 2 [ [ -LRB- 10_1101-2021_01_08_425864 722 3 47 47 CD 10_1101-2021_01_08_425864 722 4 ] ] -RRB- 10_1101-2021_01_08_425864 722 5 D. D. NNP 10_1101-2021_01_08_425864 722 6 Marciano Marciano NNP 10_1101-2021_01_08_425864 722 7 , , , 10_1101-2021_01_08_425864 722 8 C. C. NNP 10_1101-2021_01_08_425864 722 9 Llorente Llorente NNP 10_1101-2021_01_08_425864 722 10 , , , 10_1101-2021_01_08_425864 722 11 D.A. D.A. NNP 10_1101-2021_01_08_425864 723 1 Maugeri Maugeri NNP 10_1101-2021_01_08_425864 723 2 , , , 10_1101-2021_01_08_425864 723 3 C. C. NNP 10_1101-2021_01_08_425864 723 4 de de NNP 10_1101-2021_01_08_425864 723 5 la la NNP 10_1101-2021_01_08_425864 723 6 Fuente Fuente NNP 10_1101-2021_01_08_425864 723 7 , , , 10_1101-2021_01_08_425864 723 8 F. F. NNP 10_1101-2021_01_08_425864 723 9 Opperdoes Opperdoes NNP 10_1101-2021_01_08_425864 723 10 , , , 10_1101-2021_01_08_425864 723 11 J.J. J.J. NNP 10_1101-2021_01_08_425864 723 12 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 723 13 , , , 10_1101-2021_01_08_425864 723 14 C. C. NNP 10_1101-2021_01_08_425864 723 15 828 828 CD 10_1101-2021_01_08_425864 723 16 Nowicki Nowicki NNP 10_1101-2021_01_08_425864 723 17 , , , 10_1101-2021_01_08_425864 723 18 Biochemical biochemical JJ 10_1101-2021_01_08_425864 723 19 characterization characterization NN 10_1101-2021_01_08_425864 723 20 of of IN 10_1101-2021_01_08_425864 723 21 stage stage NN 10_1101-2021_01_08_425864 723 22 - - HYPH 10_1101-2021_01_08_425864 723 23 specific specific JJ 10_1101-2021_01_08_425864 723 24 isoforms isoform NNS 10_1101-2021_01_08_425864 723 25 of of IN 10_1101-2021_01_08_425864 723 26 aspartate aspartate NN 10_1101-2021_01_08_425864 723 27 829 829 CD 10_1101-2021_01_08_425864 723 28 aminotransferases aminotransferase NNS 10_1101-2021_01_08_425864 723 29 from from IN 10_1101-2021_01_08_425864 723 30 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 723 31 cruzi cruzi NNS 10_1101-2021_01_08_425864 723 32 and and CC 10_1101-2021_01_08_425864 723 33 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 723 34 brucei brucei NN 10_1101-2021_01_08_425864 723 35 , , , 10_1101-2021_01_08_425864 723 36 Mol Mol NNP 10_1101-2021_01_08_425864 723 37 . . . 10_1101-2021_01_08_425864 724 1 Biochem Biochem NNP 10_1101-2021_01_08_425864 724 2 . . . 10_1101-2021_01_08_425864 725 1 830 830 CD 10_1101-2021_01_08_425864 725 2 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 725 3 . . . 10_1101-2021_01_08_425864 726 1 161 161 CD 10_1101-2021_01_08_425864 726 2 ( ( -LRB- 10_1101-2021_01_08_425864 726 3 2008 2008 CD 10_1101-2021_01_08_425864 726 4 ) ) -RRB- 10_1101-2021_01_08_425864 726 5 12–20 12–20 CD 10_1101-2021_01_08_425864 726 6 . . . 10_1101-2021_01_08_425864 727 1 https://doi.org/10.1016/j.molbiopara.2008.05.005 https://doi.org/10.1016/j.molbiopara.2008.05.005 XX 10_1101-2021_01_08_425864 727 2 . . . 10_1101-2021_01_08_425864 728 1 831 831 CD 10_1101-2021_01_08_425864 728 2 [ [ -LRB- 10_1101-2021_01_08_425864 728 3 48 48 CD 10_1101-2021_01_08_425864 728 4 ] ] -RRB- 10_1101-2021_01_08_425864 728 5 J.J. J.J. NNP 10_1101-2021_01_08_425864 728 6 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 728 7 , , , 10_1101-2021_01_08_425864 728 8 S. S. NNP 10_1101-2021_01_08_425864 728 9 Arauzo Arauzo NNP 10_1101-2021_01_08_425864 728 10 , , , 10_1101-2021_01_08_425864 728 11 B.M. B.M. NNP 10_1101-2021_01_08_425864 729 1 Franke Franke NNP 10_1101-2021_01_08_425864 729 2 de de NNP 10_1101-2021_01_08_425864 729 3 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 729 4 , , , 10_1101-2021_01_08_425864 729 5 J.J.B. J.J.B. NNP 10_1101-2021_01_08_425864 730 1 Cannata Cannata NNP 10_1101-2021_01_08_425864 730 2 , , , 10_1101-2021_01_08_425864 730 3 On on IN 10_1101-2021_01_08_425864 730 4 the the DT 10_1101-2021_01_08_425864 730 5 production production NN 10_1101-2021_01_08_425864 730 6 of of IN 10_1101-2021_01_08_425864 730 7 832 832 CD 10_1101-2021_01_08_425864 730 8 glycerol glycerol NN 10_1101-2021_01_08_425864 730 9 and and CC 10_1101-2021_01_08_425864 730 10 l l NN 10_1101-2021_01_08_425864 730 11 - - HYPH 10_1101-2021_01_08_425864 730 12 alanine alanine NN 10_1101-2021_01_08_425864 730 13 during during IN 10_1101-2021_01_08_425864 730 14 the the DT 10_1101-2021_01_08_425864 730 15 aerobic aerobic JJ 10_1101-2021_01_08_425864 730 16 fermentation fermentation NN 10_1101-2021_01_08_425864 730 17 of of IN 10_1101-2021_01_08_425864 730 18 glucose glucose NN 10_1101-2021_01_08_425864 730 19 by by IN 10_1101-2021_01_08_425864 730 20 trypanosomatids trypanosomatid NNS 10_1101-2021_01_08_425864 730 21 , , , 10_1101-2021_01_08_425864 730 22 FEMS FEMS NNP 10_1101-2021_01_08_425864 730 23 833 833 CD 10_1101-2021_01_08_425864 730 24 Microbiol Microbiol NNP 10_1101-2021_01_08_425864 730 25 . . . 10_1101-2021_01_08_425864 731 1 Lett Lett NNP 10_1101-2021_01_08_425864 731 2 . . . 10_1101-2021_01_08_425864 732 1 51 51 CD 10_1101-2021_01_08_425864 732 2 ( ( -LRB- 10_1101-2021_01_08_425864 732 3 1988 1988 CD 10_1101-2021_01_08_425864 732 4 ) ) -RRB- 10_1101-2021_01_08_425864 732 5 187–191 187–191 CD 10_1101-2021_01_08_425864 732 6 . . . 10_1101-2021_01_08_425864 733 1 https://doi.org/10.1111/j.1574-6968.1988.tb02995.x https://doi.org/10.1111/j.1574-6968.1988.tb02995.x NNP 10_1101-2021_01_08_425864 733 2 . . . 10_1101-2021_01_08_425864 734 1 834 834 CD 10_1101-2021_01_08_425864 734 2 [ [ -LRB- 10_1101-2021_01_08_425864 734 3 49 49 CD 10_1101-2021_01_08_425864 734 4 ] ] -RRB- 10_1101-2021_01_08_425864 734 5 A. a. NN 10_1101-2021_01_08_425864 734 6 Boveris Boveris NNP 10_1101-2021_01_08_425864 734 7 , , , 10_1101-2021_01_08_425864 734 8 C.M. C.M. NNP 10_1101-2021_01_08_425864 735 1 Hertig Hertig NNP 10_1101-2021_01_08_425864 735 2 , , , 10_1101-2021_01_08_425864 735 3 J.F. J.F. NNP 10_1101-2021_01_08_425864 736 1 Turrens Turrens NNP 10_1101-2021_01_08_425864 736 2 , , , 10_1101-2021_01_08_425864 736 3 Fumarate Fumarate NNP 10_1101-2021_01_08_425864 736 4 reductase reductase NN 10_1101-2021_01_08_425864 736 5 and and CC 10_1101-2021_01_08_425864 736 6 other other JJ 10_1101-2021_01_08_425864 736 7 mitochondrial mitochondrial NN 10_1101-2021_01_08_425864 736 8 activities activity NNS 10_1101-2021_01_08_425864 736 9 835 835 CD 10_1101-2021_01_08_425864 736 10 in in IN 10_1101-2021_01_08_425864 736 11 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 736 12 cruzi cruzi NNS 10_1101-2021_01_08_425864 736 13 , , , 10_1101-2021_01_08_425864 736 14 Mol Mol NNP 10_1101-2021_01_08_425864 736 15 . . . 10_1101-2021_01_08_425864 737 1 Biochem Biochem NNP 10_1101-2021_01_08_425864 737 2 . . . 10_1101-2021_01_08_425864 738 1 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 738 2 . . . 10_1101-2021_01_08_425864 739 1 19 19 CD 10_1101-2021_01_08_425864 739 2 ( ( -LRB- 10_1101-2021_01_08_425864 739 3 1986 1986 CD 10_1101-2021_01_08_425864 739 4 ) ) -RRB- 10_1101-2021_01_08_425864 739 5 163–169 163–169 CD 10_1101-2021_01_08_425864 739 6 . . . 10_1101-2021_01_08_425864 740 1 836 836 CD 10_1101-2021_01_08_425864 740 2 https://doi.org/10.1016/0166-6851(86)90121-0 https://doi.org/10.1016/0166-6851(86)90121-0 NNPS 10_1101-2021_01_08_425864 740 3 . . . 10_1101-2021_01_08_425864 741 1 837 837 CD 10_1101-2021_01_08_425864 741 2 [ [ -LRB- 10_1101-2021_01_08_425864 741 3 50 50 CD 10_1101-2021_01_08_425864 741 4 ] ] -RRB- 10_1101-2021_01_08_425864 741 5 G.W. G.W. NNP 10_1101-2021_01_08_425864 742 1 Rogerson Rogerson NNP 10_1101-2021_01_08_425864 742 2 , , , 10_1101-2021_01_08_425864 742 3 W.E. W.E. NNP 10_1101-2021_01_08_425864 743 1 Gutteridge Gutteridge NNP 10_1101-2021_01_08_425864 743 2 , , , 10_1101-2021_01_08_425864 743 3 Catabolic catabolic JJ 10_1101-2021_01_08_425864 743 4 metabolism metabolism NN 10_1101-2021_01_08_425864 743 5 in in IN 10_1101-2021_01_08_425864 743 6 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 743 7 cruzi cruzi NNS 10_1101-2021_01_08_425864 743 8 , , , 10_1101-2021_01_08_425864 743 9 Int Int NNP 10_1101-2021_01_08_425864 743 10 . . . 10_1101-2021_01_08_425864 744 1 J. J. NNP 10_1101-2021_01_08_425864 745 1 .CC .CC NFP 10_1101-2021_01_08_425864 745 2 - - : 10_1101-2021_01_08_425864 745 3 BY by IN 10_1101-2021_01_08_425864 745 4 4.0 4.0 CD 10_1101-2021_01_08_425864 745 5 International international JJ 10_1101-2021_01_08_425864 745 6 licensemade licensemade NN 10_1101-2021_01_08_425864 745 7 available available JJ 10_1101-2021_01_08_425864 745 8 under under IN 10_1101-2021_01_08_425864 745 9 a a DT 10_1101-2021_01_08_425864 745 10 ( ( -LRB- 10_1101-2021_01_08_425864 745 11 which which WDT 10_1101-2021_01_08_425864 745 12 was be VBD 10_1101-2021_01_08_425864 745 13 not not RB 10_1101-2021_01_08_425864 745 14 certified certify VBN 10_1101-2021_01_08_425864 745 15 by by IN 10_1101-2021_01_08_425864 745 16 peer peer NN 10_1101-2021_01_08_425864 745 17 review review NN 10_1101-2021_01_08_425864 745 18 ) ) -RRB- 10_1101-2021_01_08_425864 745 19 is be VBZ 10_1101-2021_01_08_425864 745 20 the the DT 10_1101-2021_01_08_425864 745 21 author author NN 10_1101-2021_01_08_425864 745 22 / / SYM 10_1101-2021_01_08_425864 745 23 funder funder NN 10_1101-2021_01_08_425864 745 24 , , , 10_1101-2021_01_08_425864 745 25 who who WP 10_1101-2021_01_08_425864 745 26 has have VBZ 10_1101-2021_01_08_425864 745 27 granted grant VBN 10_1101-2021_01_08_425864 745 28 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 745 29 a a DT 10_1101-2021_01_08_425864 745 30 license license NN 10_1101-2021_01_08_425864 745 31 to to TO 10_1101-2021_01_08_425864 745 32 display display VB 10_1101-2021_01_08_425864 745 33 the the DT 10_1101-2021_01_08_425864 745 34 preprint preprint NN 10_1101-2021_01_08_425864 745 35 in in IN 10_1101-2021_01_08_425864 745 36 perpetuity perpetuity NN 10_1101-2021_01_08_425864 745 37 . . . 10_1101-2021_01_08_425864 746 1 It -PRON- PRP 10_1101-2021_01_08_425864 746 2 is be VBZ 10_1101-2021_01_08_425864 746 3 The the DT 10_1101-2021_01_08_425864 746 4 copyright copyright NN 10_1101-2021_01_08_425864 746 5 holder holder NN 10_1101-2021_01_08_425864 746 6 for for IN 10_1101-2021_01_08_425864 746 7 this this DT 10_1101-2021_01_08_425864 746 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 746 9 version version NN 10_1101-2021_01_08_425864 746 10 posted post VBD 10_1101-2021_01_08_425864 746 11 January January NNP 10_1101-2021_01_08_425864 746 12 8 8 CD 10_1101-2021_01_08_425864 746 13 , , , 10_1101-2021_01_08_425864 746 14 2021 2021 CD 10_1101-2021_01_08_425864 746 15 . . . 10_1101-2021_01_08_425864 746 16 ; ; : 10_1101-2021_01_08_425864 746 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 746 18 : : : 10_1101-2021_01_08_425864 746 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 746 20 preprint preprint NN 10_1101-2021_01_08_425864 746 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 746 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 746 23 838 838 CD 10_1101-2021_01_08_425864 746 24 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 746 25 . . . 10_1101-2021_01_08_425864 747 1 10 10 CD 10_1101-2021_01_08_425864 747 2 ( ( -LRB- 10_1101-2021_01_08_425864 747 3 1980 1980 CD 10_1101-2021_01_08_425864 747 4 ) ) -RRB- 10_1101-2021_01_08_425864 747 5 131–135 131–135 CD 10_1101-2021_01_08_425864 747 6 . . . 10_1101-2021_01_08_425864 748 1 https://doi.org/10.1016/0020-7519(80)90024-7 https://doi.org/10.1016/0020-7519(80)90024-7 UH 10_1101-2021_01_08_425864 748 2 . . . 10_1101-2021_01_08_425864 749 1 839 839 CD 10_1101-2021_01_08_425864 749 2 [ [ -LRB- 10_1101-2021_01_08_425864 749 3 51 51 CD 10_1101-2021_01_08_425864 749 4 ] ] -RRB- 10_1101-2021_01_08_425864 749 5 M.G. M.G. NNP 10_1101-2021_01_08_425864 750 1 Pereira Pereira NNP 10_1101-2021_01_08_425864 750 2 , , , 10_1101-2021_01_08_425864 750 3 G. G. NNP 10_1101-2021_01_08_425864 750 4 Visbal Visbal NNP 10_1101-2021_01_08_425864 750 5 , , , 10_1101-2021_01_08_425864 750 6 T.F.R. T.F.R. NNP 10_1101-2021_01_08_425864 751 1 Costa Costa NNP 10_1101-2021_01_08_425864 751 2 , , , 10_1101-2021_01_08_425864 751 3 S. S. NNP 10_1101-2021_01_08_425864 751 4 Frases Frases NNP 10_1101-2021_01_08_425864 751 5 , , , 10_1101-2021_01_08_425864 751 6 W. W. NNP 10_1101-2021_01_08_425864 751 7 de de NNP 10_1101-2021_01_08_425864 751 8 Souza Souza NNP 10_1101-2021_01_08_425864 751 9 , , , 10_1101-2021_01_08_425864 751 10 G. G. NNP 10_1101-2021_01_08_425864 751 11 Atella Atella NNP 10_1101-2021_01_08_425864 751 12 , , , 10_1101-2021_01_08_425864 751 13 N. N. NNP 10_1101-2021_01_08_425864 751 14 Cunha Cunha NNP 10_1101-2021_01_08_425864 751 15 - - HYPH 10_1101-2021_01_08_425864 751 16 e e NNP 10_1101-2021_01_08_425864 751 17 - - HYPH 10_1101-2021_01_08_425864 751 18 Silva silva NN 10_1101-2021_01_08_425864 751 19 , , , 10_1101-2021_01_08_425864 751 20 840 840 CD 10_1101-2021_01_08_425864 751 21 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 751 22 cruzi cruzi NNS 10_1101-2021_01_08_425864 751 23 epimastigotes epimastigote VBZ 10_1101-2021_01_08_425864 751 24 store store NN 10_1101-2021_01_08_425864 751 25 cholesteryl cholesteryl NN 10_1101-2021_01_08_425864 751 26 esters ester NNS 10_1101-2021_01_08_425864 751 27 in in IN 10_1101-2021_01_08_425864 751 28 lipid lipid NN 10_1101-2021_01_08_425864 751 29 droplets droplet NNS 10_1101-2021_01_08_425864 751 30 after after IN 10_1101-2021_01_08_425864 751 31 cholesterol cholesterol NN 10_1101-2021_01_08_425864 751 32 841 841 CD 10_1101-2021_01_08_425864 751 33 endocytosis endocytosis NN 10_1101-2021_01_08_425864 751 34 , , , 10_1101-2021_01_08_425864 751 35 Mol Mol NNP 10_1101-2021_01_08_425864 751 36 . . . 10_1101-2021_01_08_425864 752 1 Biochem Biochem NNP 10_1101-2021_01_08_425864 752 2 . . . 10_1101-2021_01_08_425864 753 1 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 753 2 . . . 10_1101-2021_01_08_425864 754 1 224 224 CD 10_1101-2021_01_08_425864 754 2 ( ( -LRB- 10_1101-2021_01_08_425864 754 3 2018 2018 CD 10_1101-2021_01_08_425864 754 4 ) ) -RRB- 10_1101-2021_01_08_425864 754 5 6–16 6–16 ADD 10_1101-2021_01_08_425864 754 6 . . . 10_1101-2021_01_08_425864 755 1 842 842 CD 10_1101-2021_01_08_425864 755 2 https://doi.org/10.1016/J.MOLBIOPARA.2018.07.004 https://doi.org/10.1016/J.MOLBIOPARA.2018.07.004 NNS 10_1101-2021_01_08_425864 755 3 . . . 10_1101-2021_01_08_425864 756 1 843 843 CD 10_1101-2021_01_08_425864 756 2 [ [ -LRB- 10_1101-2021_01_08_425864 756 3 52 52 CD 10_1101-2021_01_08_425864 756 4 ] ] -RRB- 10_1101-2021_01_08_425864 756 5 M.G. M.G. NNP 10_1101-2021_01_08_425864 757 1 Pereira Pereira NNP 10_1101-2021_01_08_425864 757 2 , , , 10_1101-2021_01_08_425864 757 3 G. G. NNP 10_1101-2021_01_08_425864 757 4 Visbal Visbal NNP 10_1101-2021_01_08_425864 757 5 , , , 10_1101-2021_01_08_425864 757 6 L.T. L.T. NNP 10_1101-2021_01_08_425864 758 1 Salgado Salgado NNP 10_1101-2021_01_08_425864 758 2 , , , 10_1101-2021_01_08_425864 758 3 J.C. J.C. NNP 10_1101-2021_01_08_425864 758 4 Vidal Vidal NNP 10_1101-2021_01_08_425864 758 5 , , , 10_1101-2021_01_08_425864 758 6 J.L.P. J.L.P. NNP 10_1101-2021_01_08_425864 759 1 Godinho Godinho NNP 10_1101-2021_01_08_425864 759 2 , , , 10_1101-2021_01_08_425864 759 3 N.N.T. N.N.T. NNP 10_1101-2021_01_08_425864 760 1 De De NNP 10_1101-2021_01_08_425864 760 2 Cicco Cicco NNP 10_1101-2021_01_08_425864 760 3 , , , 10_1101-2021_01_08_425864 760 4 G.C. G.C. NNP 10_1101-2021_01_08_425864 761 1 844 844 CD 10_1101-2021_01_08_425864 761 2 Atella Atella NNP 10_1101-2021_01_08_425864 761 3 , , , 10_1101-2021_01_08_425864 761 4 W. W. NNP 10_1101-2021_01_08_425864 761 5 de de NNP 10_1101-2021_01_08_425864 761 6 Souza Souza NNP 10_1101-2021_01_08_425864 761 7 , , , 10_1101-2021_01_08_425864 761 8 N. N. NNP 10_1101-2021_01_08_425864 761 9 Cunha Cunha NNP 10_1101-2021_01_08_425864 761 10 - - HYPH 10_1101-2021_01_08_425864 761 11 e e NNP 10_1101-2021_01_08_425864 761 12 - - HYPH 10_1101-2021_01_08_425864 761 13 Silva Silva NNP 10_1101-2021_01_08_425864 761 14 , , , 10_1101-2021_01_08_425864 761 15 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 761 16 cruzi cruzi NNS 10_1101-2021_01_08_425864 761 17 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 761 18 are be VBP 10_1101-2021_01_08_425864 761 19 able able JJ 10_1101-2021_01_08_425864 761 20 to to TO 10_1101-2021_01_08_425864 761 21 manage manage VB 10_1101-2021_01_08_425864 761 22 845 845 CD 10_1101-2021_01_08_425864 761 23 internal internal JJ 10_1101-2021_01_08_425864 761 24 cholesterol cholesterol NN 10_1101-2021_01_08_425864 761 25 levels level NNS 10_1101-2021_01_08_425864 761 26 under under IN 10_1101-2021_01_08_425864 761 27 nutritional nutritional JJ 10_1101-2021_01_08_425864 761 28 lipid lipid NN 10_1101-2021_01_08_425864 761 29 stress stress NN 10_1101-2021_01_08_425864 761 30 conditions condition NNS 10_1101-2021_01_08_425864 761 31 , , , 10_1101-2021_01_08_425864 761 32 PLoS PLoS NNP 10_1101-2021_01_08_425864 761 33 One one CD 10_1101-2021_01_08_425864 761 34 . . . 10_1101-2021_01_08_425864 762 1 10 10 CD 10_1101-2021_01_08_425864 762 2 ( ( -LRB- 10_1101-2021_01_08_425864 762 3 2015 2015 CD 10_1101-2021_01_08_425864 762 4 ) ) -RRB- 10_1101-2021_01_08_425864 762 5 846 846 CD 10_1101-2021_01_08_425864 762 6 e0128949 e0128949 CD 10_1101-2021_01_08_425864 762 7 . . . 10_1101-2021_01_08_425864 763 1 https://doi.org/10.1371/journal.pone.0128949 https://doi.org/10.1371/journal.pone.0128949 NNP 10_1101-2021_01_08_425864 763 2 . . . 10_1101-2021_01_08_425864 764 1 847 847 CD 10_1101-2021_01_08_425864 764 2 [ [ -LRB- 10_1101-2021_01_08_425864 764 3 53 53 CD 10_1101-2021_01_08_425864 764 4 ] ] -RRB- 10_1101-2021_01_08_425864 764 5 S. S. NNP 10_1101-2021_01_08_425864 764 6 Allmann Allmann NNP 10_1101-2021_01_08_425864 764 7 , , , 10_1101-2021_01_08_425864 764 8 M. M. NNP 10_1101-2021_01_08_425864 764 9 Mazet Mazet NNP 10_1101-2021_01_08_425864 764 10 , , , 10_1101-2021_01_08_425864 764 11 N. N. NNP 10_1101-2021_01_08_425864 764 12 Ziebart Ziebart NNP 10_1101-2021_01_08_425864 764 13 , , , 10_1101-2021_01_08_425864 764 14 G. G. NNP 10_1101-2021_01_08_425864 764 15 Bouyssou Bouyssou NNP 10_1101-2021_01_08_425864 764 16 , , , 10_1101-2021_01_08_425864 764 17 L. L. NNP 10_1101-2021_01_08_425864 764 18 Fouillen Fouillen NNP 10_1101-2021_01_08_425864 764 19 , , , 10_1101-2021_01_08_425864 764 20 J.-W. J.-W. NNP 10_1101-2021_01_08_425864 764 21 Dupuy Dupuy NNP 10_1101-2021_01_08_425864 764 22 , , , 10_1101-2021_01_08_425864 764 23 M. M. NNP 10_1101-2021_01_08_425864 764 24 Bonneu Bonneu NNP 10_1101-2021_01_08_425864 764 25 , , , 10_1101-2021_01_08_425864 764 26 P. P. NNP 10_1101-2021_01_08_425864 764 27 848 848 CD 10_1101-2021_01_08_425864 764 28 Moreau Moreau NNP 10_1101-2021_01_08_425864 764 29 , , , 10_1101-2021_01_08_425864 764 30 F. F. NNP 10_1101-2021_01_08_425864 764 31 Bringaud Bringaud NNP 10_1101-2021_01_08_425864 764 32 , , , 10_1101-2021_01_08_425864 764 33 M. M. NNP 10_1101-2021_01_08_425864 764 34 Boshart Boshart NNP 10_1101-2021_01_08_425864 764 35 , , , 10_1101-2021_01_08_425864 764 36 Triacylglycerol Triacylglycerol NNP 10_1101-2021_01_08_425864 764 37 storage storage NN 10_1101-2021_01_08_425864 764 38 in in IN 10_1101-2021_01_08_425864 764 39 lipid lipid NN 10_1101-2021_01_08_425864 764 40 droplets droplet NNS 10_1101-2021_01_08_425864 764 41 in in IN 10_1101-2021_01_08_425864 764 42 procyclic procyclic JJ 10_1101-2021_01_08_425864 764 43 849 849 CD 10_1101-2021_01_08_425864 764 44 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 764 45 brucei brucei NN 10_1101-2021_01_08_425864 764 46 , , , 10_1101-2021_01_08_425864 764 47 PLoS PLoS NNP 10_1101-2021_01_08_425864 764 48 One one CD 10_1101-2021_01_08_425864 764 49 . . . 10_1101-2021_01_08_425864 765 1 9 9 CD 10_1101-2021_01_08_425864 765 2 ( ( -LRB- 10_1101-2021_01_08_425864 765 3 2014 2014 CD 10_1101-2021_01_08_425864 765 4 ) ) -RRB- 10_1101-2021_01_08_425864 765 5 e114628 e114628 NN 10_1101-2021_01_08_425864 765 6 . . . 10_1101-2021_01_08_425864 766 1 850 850 CD 10_1101-2021_01_08_425864 766 2 https://doi.org/10.1371/journal.pone.0114628 https://doi.org/10.1371/journal.pone.0114628 IN 10_1101-2021_01_08_425864 766 3 . . . 10_1101-2021_01_08_425864 767 1 851 851 CD 10_1101-2021_01_08_425864 767 2 [ [ -LRB- 10_1101-2021_01_08_425864 767 3 54 54 CD 10_1101-2021_01_08_425864 767 4 ] ] -RRB- 10_1101-2021_01_08_425864 767 5 V.E. V.E. NNP 10_1101-2021_01_08_425864 768 1 Alvarez Alvarez NNP 10_1101-2021_01_08_425864 768 2 , , , 10_1101-2021_01_08_425864 768 3 G. G. NNP 10_1101-2021_01_08_425864 768 4 Kosec Kosec NNP 10_1101-2021_01_08_425864 768 5 , , , 10_1101-2021_01_08_425864 768 6 C. C. NNP 10_1101-2021_01_08_425864 768 7 Sant’Anna Sant’Anna NNP 10_1101-2021_01_08_425864 768 8 , , , 10_1101-2021_01_08_425864 768 9 V. V. NNP 10_1101-2021_01_08_425864 768 10 Turk Turk NNP 10_1101-2021_01_08_425864 768 11 , , , 10_1101-2021_01_08_425864 768 12 J.J. J.J. NNP 10_1101-2021_01_08_425864 768 13 Cazzulo Cazzulo NNP 10_1101-2021_01_08_425864 768 14 , , , 10_1101-2021_01_08_425864 768 15 B. B. NNP 10_1101-2021_01_08_425864 768 16 Turk Turk NNP 10_1101-2021_01_08_425864 768 17 , , , 10_1101-2021_01_08_425864 768 18 Autophagy Autophagy NNP 10_1101-2021_01_08_425864 768 19 is be VBZ 10_1101-2021_01_08_425864 768 20 852 852 CD 10_1101-2021_01_08_425864 768 21 involved involve VBN 10_1101-2021_01_08_425864 768 22 in in IN 10_1101-2021_01_08_425864 768 23 nutritional nutritional JJ 10_1101-2021_01_08_425864 768 24 stress stress NN 10_1101-2021_01_08_425864 768 25 response response NN 10_1101-2021_01_08_425864 768 26 and and CC 10_1101-2021_01_08_425864 768 27 differentiation differentiation NN 10_1101-2021_01_08_425864 768 28 in in IN 10_1101-2021_01_08_425864 768 29 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 768 30 cruzi cruzi NNS 10_1101-2021_01_08_425864 768 31 , , , 10_1101-2021_01_08_425864 768 32 J. J. NNP 10_1101-2021_01_08_425864 768 33 Biol Biol NNP 10_1101-2021_01_08_425864 768 34 . . . 10_1101-2021_01_08_425864 769 1 853 853 CD 10_1101-2021_01_08_425864 769 2 Chem Chem NNP 10_1101-2021_01_08_425864 769 3 . . . 10_1101-2021_01_08_425864 770 1 283 283 CD 10_1101-2021_01_08_425864 770 2 ( ( -LRB- 10_1101-2021_01_08_425864 770 3 2008 2008 CD 10_1101-2021_01_08_425864 770 4 ) ) -RRB- 10_1101-2021_01_08_425864 770 5 3454–3464 3454–3464 ADD 10_1101-2021_01_08_425864 770 6 . . . 10_1101-2021_01_08_425864 771 1 https://doi.org/10.1074/jbc.M708474200 https://doi.org/10.1074/jbc.M708474200 NNP 10_1101-2021_01_08_425864 771 2 . . . 10_1101-2021_01_08_425864 772 1 854 854 CD 10_1101-2021_01_08_425864 772 2 [ [ -LRB- 10_1101-2021_01_08_425864 772 3 55 55 CD 10_1101-2021_01_08_425864 772 4 ] ] -RRB- 10_1101-2021_01_08_425864 772 5 V.T. V.T. NNP 10_1101-2021_01_08_425864 773 1 Contreras Contreras NNP 10_1101-2021_01_08_425864 773 2 , , , 10_1101-2021_01_08_425864 773 3 J.M. J.M. NNP 10_1101-2021_01_08_425864 774 1 Salles salle NNS 10_1101-2021_01_08_425864 774 2 , , , 10_1101-2021_01_08_425864 774 3 N. N. NNP 10_1101-2021_01_08_425864 774 4 Thomas Thomas NNP 10_1101-2021_01_08_425864 774 5 , , , 10_1101-2021_01_08_425864 774 6 C.M. C.M. NNP 10_1101-2021_01_08_425864 775 1 Morel Morel NNP 10_1101-2021_01_08_425864 775 2 , , , 10_1101-2021_01_08_425864 775 3 S. S. NNP 10_1101-2021_01_08_425864 775 4 Goldenberg Goldenberg NNP 10_1101-2021_01_08_425864 775 5 , , , 10_1101-2021_01_08_425864 775 6 In in IN 10_1101-2021_01_08_425864 775 7 vitro vitro FW 10_1101-2021_01_08_425864 775 8 differentiation differentiation NN 10_1101-2021_01_08_425864 775 9 855 855 CD 10_1101-2021_01_08_425864 775 10 of of IN 10_1101-2021_01_08_425864 775 11 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 775 12 cruzi cruzi NNS 10_1101-2021_01_08_425864 775 13 under under IN 10_1101-2021_01_08_425864 775 14 chemically chemically RB 10_1101-2021_01_08_425864 775 15 defined define VBN 10_1101-2021_01_08_425864 775 16 conditions condition NNS 10_1101-2021_01_08_425864 775 17 , , , 10_1101-2021_01_08_425864 775 18 Mol Mol NNP 10_1101-2021_01_08_425864 775 19 Biochem Biochem NNP 10_1101-2021_01_08_425864 775 20 Parasitol Parasitol NNP 10_1101-2021_01_08_425864 775 21 . . . 10_1101-2021_01_08_425864 776 1 16 16 CD 10_1101-2021_01_08_425864 776 2 856 856 CD 10_1101-2021_01_08_425864 776 3 ( ( -LRB- 10_1101-2021_01_08_425864 776 4 1985 1985 CD 10_1101-2021_01_08_425864 776 5 ) ) -RRB- 10_1101-2021_01_08_425864 776 6 315–327 315–327 CD 10_1101-2021_01_08_425864 776 7 . . . 10_1101-2021_01_08_425864 777 1 https://www.ncbi.nlm.nih.gov/pubmed/3903496 https://www.ncbi.nlm.nih.gov/pubmed/3903496 ADD 10_1101-2021_01_08_425864 777 2 . . . 10_1101-2021_01_08_425864 778 1 857 857 CD 10_1101-2021_01_08_425864 778 2 [ [ -LRB- 10_1101-2021_01_08_425864 778 3 56 56 CD 10_1101-2021_01_08_425864 778 4 ] ] -RRB- 10_1101-2021_01_08_425864 778 5 M.J. M.J. NNP 10_1101-2021_01_08_425864 778 6 Wainszelbaum Wainszelbaum NNP 10_1101-2021_01_08_425864 778 7 , , , 10_1101-2021_01_08_425864 778 8 M.L. M.L. NNP 10_1101-2021_01_08_425864 779 1 Belaunzarán Belaunzarán NNP 10_1101-2021_01_08_425864 779 2 , , , 10_1101-2021_01_08_425864 779 3 E.M. E.M. NNP 10_1101-2021_01_08_425864 779 4 Lammel Lammel NNP 10_1101-2021_01_08_425864 779 5 , , , 10_1101-2021_01_08_425864 779 6 M. M. NNP 10_1101-2021_01_08_425864 779 7 Florin Florin NNP 10_1101-2021_01_08_425864 779 8 - - HYPH 10_1101-2021_01_08_425864 779 9 Christensen Christensen NNP 10_1101-2021_01_08_425864 779 10 , , , 10_1101-2021_01_08_425864 779 11 J. J. NNP 10_1101-2021_01_08_425864 779 12 Florin- Florin- NNP 10_1101-2021_01_08_425864 779 13 858 858 CD 10_1101-2021_01_08_425864 779 14 Christensen Christensen NNP 10_1101-2021_01_08_425864 779 15 , , , 10_1101-2021_01_08_425864 779 16 E.L.D. E.L.D. NNP 10_1101-2021_01_08_425864 780 1 Isola Isola NNP 10_1101-2021_01_08_425864 780 2 , , , 10_1101-2021_01_08_425864 780 3 Free free JJ 10_1101-2021_01_08_425864 780 4 fatty fatty NN 10_1101-2021_01_08_425864 780 5 acids acid NNS 10_1101-2021_01_08_425864 780 6 induce induce VBP 10_1101-2021_01_08_425864 780 7 cell cell NN 10_1101-2021_01_08_425864 780 8 differentiation differentiation NN 10_1101-2021_01_08_425864 780 9 to to IN 10_1101-2021_01_08_425864 780 10 infective infective VB 10_1101-2021_01_08_425864 780 11 forms form NNS 10_1101-2021_01_08_425864 780 12 in in IN 10_1101-2021_01_08_425864 780 13 859 859 CD 10_1101-2021_01_08_425864 780 14 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 780 15 cruzi cruzi NNS 10_1101-2021_01_08_425864 780 16 , , , 10_1101-2021_01_08_425864 780 17 Biochem Biochem NNP 10_1101-2021_01_08_425864 780 18 . . . 10_1101-2021_01_08_425864 781 1 J. J. NNP 10_1101-2021_01_08_425864 782 1 375 375 CD 10_1101-2021_01_08_425864 782 2 ( ( -LRB- 10_1101-2021_01_08_425864 782 3 2003 2003 CD 10_1101-2021_01_08_425864 782 4 ) ) -RRB- 10_1101-2021_01_08_425864 782 5 705–12 705–12 CD 10_1101-2021_01_08_425864 782 6 . . . 10_1101-2021_01_08_425864 783 1 https://doi.org/10.1042/BJ20021907 https://doi.org/10.1042/bj20021907 UH 10_1101-2021_01_08_425864 783 2 . . . 10_1101-2021_01_08_425864 784 1 860 860 CD 10_1101-2021_01_08_425864 784 2 [ [ -LRB- 10_1101-2021_01_08_425864 784 3 57 57 CD 10_1101-2021_01_08_425864 784 4 ] ] -RRB- 10_1101-2021_01_08_425864 784 5 C.C.P. C.C.P. NNP 10_1101-2021_01_08_425864 785 1 Aires aire NNS 10_1101-2021_01_08_425864 785 2 , , , 10_1101-2021_01_08_425864 785 3 L. L. NNP 10_1101-2021_01_08_425864 785 4 IJlst IJlst NNP 10_1101-2021_01_08_425864 785 5 , , , 10_1101-2021_01_08_425864 785 6 F. F. NNP 10_1101-2021_01_08_425864 785 7 Stet Stet NNP 10_1101-2021_01_08_425864 785 8 , , , 10_1101-2021_01_08_425864 785 9 C. C. NNP 10_1101-2021_01_08_425864 785 10 Prip Prip NNP 10_1101-2021_01_08_425864 785 11 - - HYPH 10_1101-2021_01_08_425864 785 12 Buus Buus NNP 10_1101-2021_01_08_425864 785 13 , , , 10_1101-2021_01_08_425864 785 14 I.T. I.T. NNP 10_1101-2021_01_08_425864 786 1 de de NNP 10_1101-2021_01_08_425864 786 2 Almeida Almeida NNP 10_1101-2021_01_08_425864 786 3 , , , 10_1101-2021_01_08_425864 786 4 M. M. NNP 10_1101-2021_01_08_425864 786 5 Duran Duran NNP 10_1101-2021_01_08_425864 786 6 , , , 10_1101-2021_01_08_425864 786 7 R.J.A. R.J.A. NNP 10_1101-2021_01_08_425864 787 1 Wanders wander NNS 10_1101-2021_01_08_425864 787 2 , , , 10_1101-2021_01_08_425864 787 3 861 861 CD 10_1101-2021_01_08_425864 787 4 M.F.B. M.F.B. NNP 10_1101-2021_01_08_425864 788 1 Silva silva NN 10_1101-2021_01_08_425864 788 2 , , , 10_1101-2021_01_08_425864 788 3 Inhibition inhibition NN 10_1101-2021_01_08_425864 788 4 of of IN 10_1101-2021_01_08_425864 788 5 hepatic hepatic JJ 10_1101-2021_01_08_425864 788 6 carnitine carnitine NN 10_1101-2021_01_08_425864 788 7 palmitoyl palmitoyl NN 10_1101-2021_01_08_425864 788 8 - - HYPH 10_1101-2021_01_08_425864 788 9 transferase transferase NN 10_1101-2021_01_08_425864 788 10 I -PRON- PRP 10_1101-2021_01_08_425864 788 11 ( ( -LRB- 10_1101-2021_01_08_425864 788 12 CPT CPT NNP 10_1101-2021_01_08_425864 788 13 IA IA NNP 10_1101-2021_01_08_425864 788 14 ) ) -RRB- 10_1101-2021_01_08_425864 788 15 by by IN 10_1101-2021_01_08_425864 788 16 valproyl- valproyl- NN 10_1101-2021_01_08_425864 788 17 862 862 CD 10_1101-2021_01_08_425864 788 18 CoA CoA NNP 10_1101-2021_01_08_425864 788 19 as as IN 10_1101-2021_01_08_425864 788 20 a a DT 10_1101-2021_01_08_425864 788 21 possible possible JJ 10_1101-2021_01_08_425864 788 22 mechanism mechanism NN 10_1101-2021_01_08_425864 788 23 of of IN 10_1101-2021_01_08_425864 788 24 valproate valproate RB 10_1101-2021_01_08_425864 788 25 - - HYPH 10_1101-2021_01_08_425864 788 26 induced induce VBN 10_1101-2021_01_08_425864 788 27 steatosis steatosis NN 10_1101-2021_01_08_425864 788 28 , , , 10_1101-2021_01_08_425864 788 29 Biochem Biochem NNP 10_1101-2021_01_08_425864 788 30 . . . 10_1101-2021_01_08_425864 789 1 Pharmacol Pharmacol NNP 10_1101-2021_01_08_425864 789 2 . . . 10_1101-2021_01_08_425864 790 1 79 79 CD 10_1101-2021_01_08_425864 790 2 ( ( -LRB- 10_1101-2021_01_08_425864 790 3 2010 2010 CD 10_1101-2021_01_08_425864 790 4 ) ) -RRB- 10_1101-2021_01_08_425864 790 5 863 863 CD 10_1101-2021_01_08_425864 790 6 792–799 792–799 CD 10_1101-2021_01_08_425864 790 7 . . . 10_1101-2021_01_08_425864 791 1 https://doi.org/10.1016/j.bcp.2009.10.011 https://doi.org/10.1016/j.bcp.2009.10.011 NNP 10_1101-2021_01_08_425864 791 2 . . . 10_1101-2021_01_08_425864 792 1 .CC .CC NFP 10_1101-2021_01_08_425864 792 2 - - : 10_1101-2021_01_08_425864 792 3 BY by IN 10_1101-2021_01_08_425864 792 4 4.0 4.0 CD 10_1101-2021_01_08_425864 792 5 International international JJ 10_1101-2021_01_08_425864 792 6 licensemade licensemade NN 10_1101-2021_01_08_425864 792 7 available available JJ 10_1101-2021_01_08_425864 792 8 under under IN 10_1101-2021_01_08_425864 792 9 a a DT 10_1101-2021_01_08_425864 792 10 ( ( -LRB- 10_1101-2021_01_08_425864 792 11 which which WDT 10_1101-2021_01_08_425864 792 12 was be VBD 10_1101-2021_01_08_425864 792 13 not not RB 10_1101-2021_01_08_425864 792 14 certified certify VBN 10_1101-2021_01_08_425864 792 15 by by IN 10_1101-2021_01_08_425864 792 16 peer peer NN 10_1101-2021_01_08_425864 792 17 review review NN 10_1101-2021_01_08_425864 792 18 ) ) -RRB- 10_1101-2021_01_08_425864 792 19 is be VBZ 10_1101-2021_01_08_425864 792 20 the the DT 10_1101-2021_01_08_425864 792 21 author author NN 10_1101-2021_01_08_425864 792 22 / / SYM 10_1101-2021_01_08_425864 792 23 funder funder NN 10_1101-2021_01_08_425864 792 24 , , , 10_1101-2021_01_08_425864 792 25 who who WP 10_1101-2021_01_08_425864 792 26 has have VBZ 10_1101-2021_01_08_425864 792 27 granted grant VBN 10_1101-2021_01_08_425864 792 28 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 792 29 a a DT 10_1101-2021_01_08_425864 792 30 license license NN 10_1101-2021_01_08_425864 792 31 to to TO 10_1101-2021_01_08_425864 792 32 display display VB 10_1101-2021_01_08_425864 792 33 the the DT 10_1101-2021_01_08_425864 792 34 preprint preprint NN 10_1101-2021_01_08_425864 792 35 in in IN 10_1101-2021_01_08_425864 792 36 perpetuity perpetuity NN 10_1101-2021_01_08_425864 792 37 . . . 10_1101-2021_01_08_425864 793 1 It -PRON- PRP 10_1101-2021_01_08_425864 793 2 is be VBZ 10_1101-2021_01_08_425864 793 3 The the DT 10_1101-2021_01_08_425864 793 4 copyright copyright NN 10_1101-2021_01_08_425864 793 5 holder holder NN 10_1101-2021_01_08_425864 793 6 for for IN 10_1101-2021_01_08_425864 793 7 this this DT 10_1101-2021_01_08_425864 793 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 793 9 version version NN 10_1101-2021_01_08_425864 793 10 posted post VBD 10_1101-2021_01_08_425864 793 11 January January NNP 10_1101-2021_01_08_425864 793 12 8 8 CD 10_1101-2021_01_08_425864 793 13 , , , 10_1101-2021_01_08_425864 793 14 2021 2021 CD 10_1101-2021_01_08_425864 793 15 . . . 10_1101-2021_01_08_425864 793 16 ; ; : 10_1101-2021_01_08_425864 793 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 793 18 : : : 10_1101-2021_01_08_425864 793 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 793 20 preprint preprint NN 10_1101-2021_01_08_425864 793 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 793 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 793 23 864 864 CD 10_1101-2021_01_08_425864 793 24 [ [ -LRB- 10_1101-2021_01_08_425864 793 25 58 58 CD 10_1101-2021_01_08_425864 793 26 ] ] -RRB- 10_1101-2021_01_08_425864 793 27 P.F. P.F. NNP 10_1101-2021_01_08_425864 794 1 Kantor Kantor NNP 10_1101-2021_01_08_425864 794 2 , , , 10_1101-2021_01_08_425864 794 3 A. a. NN 10_1101-2021_01_08_425864 794 4 Lucien Lucien NNP 10_1101-2021_01_08_425864 794 5 , , , 10_1101-2021_01_08_425864 794 6 R. R. NNP 10_1101-2021_01_08_425864 794 7 Kozak Kozak NNP 10_1101-2021_01_08_425864 794 8 , , , 10_1101-2021_01_08_425864 794 9 G.D. G.D. NNP 10_1101-2021_01_08_425864 794 10 Lopaschuk Lopaschuk NNP 10_1101-2021_01_08_425864 794 11 , , , 10_1101-2021_01_08_425864 794 12 The the DT 10_1101-2021_01_08_425864 794 13 antianginal antianginal JJ 10_1101-2021_01_08_425864 794 14 drug drug NN 10_1101-2021_01_08_425864 794 15 trimetazidine trimetazidine NN 10_1101-2021_01_08_425864 794 16 shifts shift NNS 10_1101-2021_01_08_425864 794 17 865 865 CD 10_1101-2021_01_08_425864 794 18 cardiac cardiac JJ 10_1101-2021_01_08_425864 794 19 energy energy NN 10_1101-2021_01_08_425864 794 20 metabolism metabolism NN 10_1101-2021_01_08_425864 794 21 from from IN 10_1101-2021_01_08_425864 794 22 fatty fatty NN 10_1101-2021_01_08_425864 794 23 acid acid NN 10_1101-2021_01_08_425864 794 24 oxidation oxidation NN 10_1101-2021_01_08_425864 794 25 to to TO 10_1101-2021_01_08_425864 794 26 glucose glucose VB 10_1101-2021_01_08_425864 794 27 oxidation oxidation NN 10_1101-2021_01_08_425864 794 28 by by IN 10_1101-2021_01_08_425864 794 29 inhibiting inhibit VBG 10_1101-2021_01_08_425864 794 30 866 866 CD 10_1101-2021_01_08_425864 794 31 mitochondrial mitochondrial JJ 10_1101-2021_01_08_425864 794 32 long long JJ 10_1101-2021_01_08_425864 794 33 - - HYPH 10_1101-2021_01_08_425864 794 34 chain chain NN 10_1101-2021_01_08_425864 794 35 3-ketoacyl 3-ketoacyl CD 10_1101-2021_01_08_425864 794 36 coenzyme coenzyme NNS 10_1101-2021_01_08_425864 794 37 A a DT 10_1101-2021_01_08_425864 794 38 thiolase thiolase NN 10_1101-2021_01_08_425864 794 39 , , , 10_1101-2021_01_08_425864 794 40 Circ Circ NNP 10_1101-2021_01_08_425864 794 41 . . . 10_1101-2021_01_08_425864 795 1 Res Res NNP 10_1101-2021_01_08_425864 795 2 . . . 10_1101-2021_01_08_425864 796 1 86 86 CD 10_1101-2021_01_08_425864 796 2 ( ( -LRB- 10_1101-2021_01_08_425864 796 3 2000 2000 CD 10_1101-2021_01_08_425864 796 4 ) ) -RRB- 10_1101-2021_01_08_425864 796 5 580–588 580–588 CD 10_1101-2021_01_08_425864 796 6 . . . 10_1101-2021_01_08_425864 797 1 867 867 CD 10_1101-2021_01_08_425864 797 2 https://doi.org/10.1161/01.RES.86.5.580 https://doi.org/10.1161/01.res.86.5.580 NN 10_1101-2021_01_08_425864 797 3 . . . 10_1101-2021_01_08_425864 798 1 868 868 CD 10_1101-2021_01_08_425864 798 2 [ [ -LRB- 10_1101-2021_01_08_425864 798 3 59 59 CD 10_1101-2021_01_08_425864 798 4 ] ] -RRB- 10_1101-2021_01_08_425864 798 5 C.D.L. c.d.l. NN 10_1101-2021_01_08_425864 799 1 Folmes Folmes NNP 10_1101-2021_01_08_425864 799 2 , , , 10_1101-2021_01_08_425864 799 3 A.S. A.S. NNP 10_1101-2021_01_08_425864 799 4 Clanachan Clanachan NNP 10_1101-2021_01_08_425864 799 5 , , , 10_1101-2021_01_08_425864 799 6 G.D. G.D. NNP 10_1101-2021_01_08_425864 799 7 Lopaschuk Lopaschuk NNP 10_1101-2021_01_08_425864 799 8 , , , 10_1101-2021_01_08_425864 799 9 Fatty Fatty NNP 10_1101-2021_01_08_425864 799 10 acid acid NN 10_1101-2021_01_08_425864 799 11 oxidation oxidation NN 10_1101-2021_01_08_425864 799 12 inhibitors inhibitor NNS 10_1101-2021_01_08_425864 799 13 in in IN 10_1101-2021_01_08_425864 799 14 the the DT 10_1101-2021_01_08_425864 799 15 869 869 CD 10_1101-2021_01_08_425864 799 16 management management NN 10_1101-2021_01_08_425864 799 17 of of IN 10_1101-2021_01_08_425864 799 18 chronic chronic JJ 10_1101-2021_01_08_425864 799 19 complications complication NNS 10_1101-2021_01_08_425864 799 20 of of IN 10_1101-2021_01_08_425864 799 21 atherosclerosis atherosclerosis NN 10_1101-2021_01_08_425864 799 22 , , , 10_1101-2021_01_08_425864 799 23 Curr curr UH 10_1101-2021_01_08_425864 799 24 . . . 10_1101-2021_01_08_425864 800 1 Atheroscler Atheroscler NNP 10_1101-2021_01_08_425864 800 2 . . . 10_1101-2021_01_08_425864 801 1 Rep. Rep. NNP 10_1101-2021_01_08_425864 801 2 7 7 CD 10_1101-2021_01_08_425864 801 3 ( ( -LRB- 10_1101-2021_01_08_425864 801 4 2005 2005 CD 10_1101-2021_01_08_425864 801 5 ) ) -RRB- 10_1101-2021_01_08_425864 801 6 870 870 CD 10_1101-2021_01_08_425864 801 7 63–70 63–70 CD 10_1101-2021_01_08_425864 801 8 . . . 10_1101-2021_01_08_425864 802 1 https://doi.org/10.1007/s11883-005-0077-2 https://doi.org/10.1007/s11883-005-0077-2 CD 10_1101-2021_01_08_425864 802 2 . . . 10_1101-2021_01_08_425864 803 1 871 871 CD 10_1101-2021_01_08_425864 803 2 [ [ -LRB- 10_1101-2021_01_08_425864 803 3 60 60 CD 10_1101-2021_01_08_425864 803 4 ] ] -RRB- 10_1101-2021_01_08_425864 803 5 W.C. W.C. NNP 10_1101-2021_01_08_425864 803 6 Stanley Stanley NNP 10_1101-2021_01_08_425864 803 7 , , , 10_1101-2021_01_08_425864 803 8 S.R. S.R. NNP 10_1101-2021_01_08_425864 804 1 Meadows Meadows NNP 10_1101-2021_01_08_425864 804 2 , , , 10_1101-2021_01_08_425864 804 3 K.M. K.M. NNP 10_1101-2021_01_08_425864 805 1 Kivilo Kivilo NNP 10_1101-2021_01_08_425864 805 2 , , , 10_1101-2021_01_08_425864 805 3 B.A. B.A. NNP 10_1101-2021_01_08_425864 806 1 Roth Roth NNP 10_1101-2021_01_08_425864 806 2 , , , 10_1101-2021_01_08_425864 806 3 G.D. G.D. NNP 10_1101-2021_01_08_425864 806 4 Lopaschuk Lopaschuk NNP 10_1101-2021_01_08_425864 806 5 , , , 10_1101-2021_01_08_425864 806 6 β β NNP 10_1101-2021_01_08_425864 806 7 - - HYPH 10_1101-2021_01_08_425864 806 8 Hydroxybutyrate Hydroxybutyrate NNP 10_1101-2021_01_08_425864 806 9 872 872 CD 10_1101-2021_01_08_425864 806 10 inhibits inhibit VBZ 10_1101-2021_01_08_425864 806 11 myocardial myocardial JJ 10_1101-2021_01_08_425864 806 12 fatty fatty NN 10_1101-2021_01_08_425864 806 13 acid acid NN 10_1101-2021_01_08_425864 806 14 oxidation oxidation NN 10_1101-2021_01_08_425864 806 15 in in IN 10_1101-2021_01_08_425864 806 16 vivo vivo NNP 10_1101-2021_01_08_425864 806 17 independent independent JJ 10_1101-2021_01_08_425864 806 18 of of IN 10_1101-2021_01_08_425864 806 19 changes change NNS 10_1101-2021_01_08_425864 806 20 in in IN 10_1101-2021_01_08_425864 806 21 malonyl malonyl NNP 10_1101-2021_01_08_425864 806 22 - - HYPH 10_1101-2021_01_08_425864 806 23 CoA CoA NNP 10_1101-2021_01_08_425864 806 24 873 873 CD 10_1101-2021_01_08_425864 806 25 content content NN 10_1101-2021_01_08_425864 806 26 , , , 10_1101-2021_01_08_425864 806 27 Am Am NNP 10_1101-2021_01_08_425864 806 28 . . . 10_1101-2021_01_08_425864 807 1 J. J. NNP 10_1101-2021_01_08_425864 807 2 Physiol Physiol NNP 10_1101-2021_01_08_425864 807 3 . . . 10_1101-2021_01_08_425864 808 1 Circ Circ NNP 10_1101-2021_01_08_425864 808 2 . . . 10_1101-2021_01_08_425864 809 1 Physiol Physiol NNP 10_1101-2021_01_08_425864 809 2 . . . 10_1101-2021_01_08_425864 810 1 285 285 CD 10_1101-2021_01_08_425864 810 2 ( ( -LRB- 10_1101-2021_01_08_425864 810 3 2003 2003 CD 10_1101-2021_01_08_425864 810 4 ) ) -RRB- 10_1101-2021_01_08_425864 810 5 H1626–H1631 h1626–h1631 UH 10_1101-2021_01_08_425864 810 6 . . . 10_1101-2021_01_08_425864 811 1 874 874 CD 10_1101-2021_01_08_425864 811 2 https://doi.org/10.1152/ajpheart.00332.2003 https://doi.org/10.1152/ajpheart.00332.2003 NN 10_1101-2021_01_08_425864 811 3 . . . 10_1101-2021_01_08_425864 812 1 875 875 CD 10_1101-2021_01_08_425864 812 2 [ [ -LRB- 10_1101-2021_01_08_425864 812 3 61 61 CD 10_1101-2021_01_08_425864 812 4 ] ] -RRB- 10_1101-2021_01_08_425864 812 5 R.S. R.S. NNP 10_1101-2021_01_08_425864 813 1 O’Connor O’Connor NNP 10_1101-2021_01_08_425864 813 2 , , , 10_1101-2021_01_08_425864 813 3 L. L. NNP 10_1101-2021_01_08_425864 813 4 Guo Guo NNP 10_1101-2021_01_08_425864 813 5 , , , 10_1101-2021_01_08_425864 813 6 S. S. NNP 10_1101-2021_01_08_425864 813 7 Ghassemi Ghassemi NNP 10_1101-2021_01_08_425864 813 8 , , , 10_1101-2021_01_08_425864 813 9 N.W. N.W. NNP 10_1101-2021_01_08_425864 814 1 Snyder Snyder NNP 10_1101-2021_01_08_425864 814 2 , , , 10_1101-2021_01_08_425864 814 3 A.J. A.J. NNP 10_1101-2021_01_08_425864 815 1 Worth worth JJ 10_1101-2021_01_08_425864 815 2 , , , 10_1101-2021_01_08_425864 815 3 L. L. NNP 10_1101-2021_01_08_425864 815 4 Weng Weng NNP 10_1101-2021_01_08_425864 815 5 , , , 10_1101-2021_01_08_425864 815 6 Y. Y. NNP 10_1101-2021_01_08_425864 815 7 Kam Kam NNP 10_1101-2021_01_08_425864 815 8 , , , 10_1101-2021_01_08_425864 815 9 B. B. NNP 10_1101-2021_01_08_425864 816 1 876 876 CD 10_1101-2021_01_08_425864 816 2 Philipson Philipson NNP 10_1101-2021_01_08_425864 816 3 , , , 10_1101-2021_01_08_425864 816 4 S. S. NNP 10_1101-2021_01_08_425864 816 5 Trefely trefely RB 10_1101-2021_01_08_425864 816 6 , , , 10_1101-2021_01_08_425864 816 7 S. S. NNP 10_1101-2021_01_08_425864 816 8 Nunez Nunez NNP 10_1101-2021_01_08_425864 816 9 - - HYPH 10_1101-2021_01_08_425864 816 10 Cruz Cruz NNP 10_1101-2021_01_08_425864 816 11 , , , 10_1101-2021_01_08_425864 816 12 I.A. I.A. NNP 10_1101-2021_01_08_425864 817 1 Blair Blair NNP 10_1101-2021_01_08_425864 817 2 , , , 10_1101-2021_01_08_425864 817 3 C.H. C.H. NNP 10_1101-2021_01_08_425864 818 1 June June NNP 10_1101-2021_01_08_425864 818 2 , , , 10_1101-2021_01_08_425864 818 3 M.C. M.C. NNP 10_1101-2021_01_08_425864 819 1 Milone Milone NNP 10_1101-2021_01_08_425864 819 2 , , , 10_1101-2021_01_08_425864 819 3 The the DT 10_1101-2021_01_08_425864 819 4 CPT1a CPT1a NNP 10_1101-2021_01_08_425864 819 5 877 877 CD 10_1101-2021_01_08_425864 819 6 inhibitor inhibitor NN 10_1101-2021_01_08_425864 819 7 , , , 10_1101-2021_01_08_425864 819 8 etomoxir etomoxir NNP 10_1101-2021_01_08_425864 819 9 induces induce VBZ 10_1101-2021_01_08_425864 819 10 severe severe JJ 10_1101-2021_01_08_425864 819 11 oxidative oxidative JJ 10_1101-2021_01_08_425864 819 12 stress stress NN 10_1101-2021_01_08_425864 819 13 at at IN 10_1101-2021_01_08_425864 819 14 commonly commonly RB 10_1101-2021_01_08_425864 819 15 used use VBN 10_1101-2021_01_08_425864 819 16 concentrations concentration NNS 10_1101-2021_01_08_425864 819 17 , , , 10_1101-2021_01_08_425864 819 18 Sci Sci NNP 10_1101-2021_01_08_425864 819 19 . . . 10_1101-2021_01_08_425864 820 1 878 878 CD 10_1101-2021_01_08_425864 820 2 Rep. Rep. NNP 10_1101-2021_01_08_425864 820 3 8 8 CD 10_1101-2021_01_08_425864 820 4 ( ( -LRB- 10_1101-2021_01_08_425864 820 5 2018 2018 CD 10_1101-2021_01_08_425864 820 6 ) ) -RRB- 10_1101-2021_01_08_425864 820 7 6289 6289 CD 10_1101-2021_01_08_425864 820 8 . . . 10_1101-2021_01_08_425864 820 9 https://doi.org/10.1038/s41598-018-24676-6 https://doi.org/10.1038/s41598-018-24676-6 UH 10_1101-2021_01_08_425864 820 10 . . . 10_1101-2021_01_08_425864 821 1 879 879 CD 10_1101-2021_01_08_425864 821 2 [ [ -LRB- 10_1101-2021_01_08_425864 821 3 62 62 CD 10_1101-2021_01_08_425864 821 4 ] ] -RRB- 10_1101-2021_01_08_425864 821 5 C.-H. C.-H. NNP 10_1101-2021_01_08_425864 821 6 Yao Yao NNP 10_1101-2021_01_08_425864 821 7 , , , 10_1101-2021_01_08_425864 821 8 G.-Y. G.-Y. NNP 10_1101-2021_01_08_425864 822 1 Liu Liu NNP 10_1101-2021_01_08_425864 822 2 , , , 10_1101-2021_01_08_425864 822 3 R. R. NNP 10_1101-2021_01_08_425864 822 4 Wang Wang NNP 10_1101-2021_01_08_425864 822 5 , , , 10_1101-2021_01_08_425864 822 6 S.H. S.H. NNP 10_1101-2021_01_08_425864 823 1 Moon Moon NNP 10_1101-2021_01_08_425864 823 2 , , , 10_1101-2021_01_08_425864 823 3 R.W. R.W. NNP 10_1101-2021_01_08_425864 824 1 Gross Gross NNP 10_1101-2021_01_08_425864 824 2 , , , 10_1101-2021_01_08_425864 824 3 G.J. G.J. NNP 10_1101-2021_01_08_425864 825 1 Patti Patti NNP 10_1101-2021_01_08_425864 825 2 , , , 10_1101-2021_01_08_425864 825 3 Identifying identify VBG 10_1101-2021_01_08_425864 825 4 off off IN 10_1101-2021_01_08_425864 825 5 - - HYPH 10_1101-2021_01_08_425864 825 6 target target NN 10_1101-2021_01_08_425864 825 7 880 880 CD 10_1101-2021_01_08_425864 825 8 effects effect NNS 10_1101-2021_01_08_425864 825 9 of of IN 10_1101-2021_01_08_425864 825 10 etomoxir etomoxir NNS 10_1101-2021_01_08_425864 825 11 reveals reveal VBZ 10_1101-2021_01_08_425864 825 12 that that IN 10_1101-2021_01_08_425864 825 13 carnitine carnitine NN 10_1101-2021_01_08_425864 825 14 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 825 15 I -PRON- PRP 10_1101-2021_01_08_425864 825 16 is be VBZ 10_1101-2021_01_08_425864 825 17 essential essential JJ 10_1101-2021_01_08_425864 825 18 for for IN 10_1101-2021_01_08_425864 825 19 cancer cancer NN 10_1101-2021_01_08_425864 825 20 cell cell NN 10_1101-2021_01_08_425864 825 21 881 881 CD 10_1101-2021_01_08_425864 825 22 proliferation proliferation NN 10_1101-2021_01_08_425864 825 23 independent independent JJ 10_1101-2021_01_08_425864 825 24 of of IN 10_1101-2021_01_08_425864 825 25 β β NN 10_1101-2021_01_08_425864 825 26 - - HYPH 10_1101-2021_01_08_425864 825 27 oxidation oxidation NN 10_1101-2021_01_08_425864 825 28 , , , 10_1101-2021_01_08_425864 825 29 PLOS PLOS NNP 10_1101-2021_01_08_425864 825 30 Biol Biol NNP 10_1101-2021_01_08_425864 825 31 . . . 10_1101-2021_01_08_425864 826 1 16 16 CD 10_1101-2021_01_08_425864 826 2 ( ( -LRB- 10_1101-2021_01_08_425864 826 3 2018 2018 CD 10_1101-2021_01_08_425864 826 4 ) ) -RRB- 10_1101-2021_01_08_425864 826 5 e2003782 e2003782 NN 10_1101-2021_01_08_425864 826 6 . . . 10_1101-2021_01_08_425864 827 1 882 882 CD 10_1101-2021_01_08_425864 827 2 https://doi.org/10.1371/journal.pbio.2003782 https://doi.org/10.1371/journal.pbio.2003782 NN 10_1101-2021_01_08_425864 827 3 . . . 10_1101-2021_01_08_425864 828 1 883 883 CD 10_1101-2021_01_08_425864 828 2 [ [ -LRB- 10_1101-2021_01_08_425864 828 3 63 63 CD 10_1101-2021_01_08_425864 828 4 ] ] -RRB- 10_1101-2021_01_08_425864 828 5 S.N. S.N. NNP 10_1101-2021_01_08_425864 829 1 Rampersad rampersad NN 10_1101-2021_01_08_425864 829 2 , , , 10_1101-2021_01_08_425864 829 3 Multiple multiple JJ 10_1101-2021_01_08_425864 829 4 applications application NNS 10_1101-2021_01_08_425864 829 5 of of IN 10_1101-2021_01_08_425864 829 6 Alamar Alamar NNP 10_1101-2021_01_08_425864 829 7 Blue Blue NNP 10_1101-2021_01_08_425864 829 8 as as IN 10_1101-2021_01_08_425864 829 9 an an DT 10_1101-2021_01_08_425864 829 10 indicator indicator NN 10_1101-2021_01_08_425864 829 11 of of IN 10_1101-2021_01_08_425864 829 12 metabolic metabolic JJ 10_1101-2021_01_08_425864 829 13 function function NN 10_1101-2021_01_08_425864 829 14 884 884 CD 10_1101-2021_01_08_425864 829 15 and and CC 10_1101-2021_01_08_425864 829 16 cellular cellular JJ 10_1101-2021_01_08_425864 829 17 health health NN 10_1101-2021_01_08_425864 829 18 in in IN 10_1101-2021_01_08_425864 829 19 cell cell NN 10_1101-2021_01_08_425864 829 20 viability viability NN 10_1101-2021_01_08_425864 829 21 bioassays bioassay NNS 10_1101-2021_01_08_425864 829 22 , , , 10_1101-2021_01_08_425864 829 23 Sensors Sensors NNP 10_1101-2021_01_08_425864 829 24 ( ( -LRB- 10_1101-2021_01_08_425864 829 25 Basel Basel NNP 10_1101-2021_01_08_425864 829 26 ) ) -RRB- 10_1101-2021_01_08_425864 829 27 . . . 10_1101-2021_01_08_425864 830 1 12 12 CD 10_1101-2021_01_08_425864 830 2 ( ( -LRB- 10_1101-2021_01_08_425864 830 3 2012 2012 CD 10_1101-2021_01_08_425864 830 4 ) ) -RRB- 10_1101-2021_01_08_425864 830 5 12347–60 12347–60 CD 10_1101-2021_01_08_425864 830 6 . . . 10_1101-2021_01_08_425864 831 1 885 885 CD 10_1101-2021_01_08_425864 831 2 https://doi.org/10.3390/s120912347 https://doi.org/10.3390/s120912347 NN 10_1101-2021_01_08_425864 831 3 . . . 10_1101-2021_01_08_425864 832 1 886 886 CD 10_1101-2021_01_08_425864 832 2 [ [ -LRB- 10_1101-2021_01_08_425864 832 3 64 64 CD 10_1101-2021_01_08_425864 832 4 ] ] -RRB- 10_1101-2021_01_08_425864 832 5 J.J. J.J. NNP 10_1101-2021_01_08_425864 832 6 Homsy Homsy NNP 10_1101-2021_01_08_425864 832 7 , , , 10_1101-2021_01_08_425864 832 8 B. B. NNP 10_1101-2021_01_08_425864 832 9 Granger Granger NNP 10_1101-2021_01_08_425864 832 10 , , , 10_1101-2021_01_08_425864 832 11 S.M. S.M. NNP 10_1101-2021_01_08_425864 833 1 Krassner Krassner NNP 10_1101-2021_01_08_425864 833 2 , , , 10_1101-2021_01_08_425864 833 3 Some some DT 10_1101-2021_01_08_425864 833 4 factors factor NNS 10_1101-2021_01_08_425864 833 5 inducing induce VBG 10_1101-2021_01_08_425864 833 6 formation formation NN 10_1101-2021_01_08_425864 833 7 of of IN 10_1101-2021_01_08_425864 833 8 metacyclic metacyclic JJ 10_1101-2021_01_08_425864 833 9 887 887 CD 10_1101-2021_01_08_425864 833 10 stages stage NNS 10_1101-2021_01_08_425864 833 11 of of IN 10_1101-2021_01_08_425864 833 12 Trypanosoma Trypanosoma NNP 10_1101-2021_01_08_425864 833 13 cruzi cruzi NNS 10_1101-2021_01_08_425864 833 14 , , , 10_1101-2021_01_08_425864 833 15 J. J. NNP 10_1101-2021_01_08_425864 833 16 Protozool Protozool NNP 10_1101-2021_01_08_425864 833 17 . . . 10_1101-2021_01_08_425864 834 1 36 36 CD 10_1101-2021_01_08_425864 834 2 ( ( -LRB- 10_1101-2021_01_08_425864 834 3 1989 1989 CD 10_1101-2021_01_08_425864 834 4 ) ) -RRB- 10_1101-2021_01_08_425864 834 5 150–3 150–3 CD 10_1101-2021_01_08_425864 834 6 . . . 10_1101-2021_01_08_425864 835 1 888 888 CD 10_1101-2021_01_08_425864 835 2 http://www.ncbi.nlm.nih.gov/pubmed/2657033 http://www.ncbi.nlm.nih.gov/pubmed/2657033 NNP 10_1101-2021_01_08_425864 835 3 ( ( -LRB- 10_1101-2021_01_08_425864 835 4 accessed access VBN 10_1101-2021_01_08_425864 835 5 January January NNP 10_1101-2021_01_08_425864 835 6 29 29 CD 10_1101-2021_01_08_425864 835 7 , , , 10_1101-2021_01_08_425864 835 8 2019 2019 CD 10_1101-2021_01_08_425864 835 9 ) ) -RRB- 10_1101-2021_01_08_425864 835 10 . . . 10_1101-2021_01_08_425864 836 1 889 889 CD 10_1101-2021_01_08_425864 836 2 890 890 CD 10_1101-2021_01_08_425864 836 3 .CC .CC : 10_1101-2021_01_08_425864 836 4 - - : 10_1101-2021_01_08_425864 836 5 BY by IN 10_1101-2021_01_08_425864 836 6 4.0 4.0 CD 10_1101-2021_01_08_425864 836 7 International international JJ 10_1101-2021_01_08_425864 836 8 licensemade licensemade NN 10_1101-2021_01_08_425864 836 9 available available JJ 10_1101-2021_01_08_425864 836 10 under under IN 10_1101-2021_01_08_425864 836 11 a a DT 10_1101-2021_01_08_425864 836 12 ( ( -LRB- 10_1101-2021_01_08_425864 836 13 which which WDT 10_1101-2021_01_08_425864 836 14 was be VBD 10_1101-2021_01_08_425864 836 15 not not RB 10_1101-2021_01_08_425864 836 16 certified certify VBN 10_1101-2021_01_08_425864 836 17 by by IN 10_1101-2021_01_08_425864 836 18 peer peer NN 10_1101-2021_01_08_425864 836 19 review review NN 10_1101-2021_01_08_425864 836 20 ) ) -RRB- 10_1101-2021_01_08_425864 836 21 is be VBZ 10_1101-2021_01_08_425864 836 22 the the DT 10_1101-2021_01_08_425864 836 23 author author NN 10_1101-2021_01_08_425864 836 24 / / SYM 10_1101-2021_01_08_425864 836 25 funder funder NN 10_1101-2021_01_08_425864 836 26 , , , 10_1101-2021_01_08_425864 836 27 who who WP 10_1101-2021_01_08_425864 836 28 has have VBZ 10_1101-2021_01_08_425864 836 29 granted grant VBN 10_1101-2021_01_08_425864 836 30 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 836 31 a a DT 10_1101-2021_01_08_425864 836 32 license license NN 10_1101-2021_01_08_425864 836 33 to to TO 10_1101-2021_01_08_425864 836 34 display display VB 10_1101-2021_01_08_425864 836 35 the the DT 10_1101-2021_01_08_425864 836 36 preprint preprint NN 10_1101-2021_01_08_425864 836 37 in in IN 10_1101-2021_01_08_425864 836 38 perpetuity perpetuity NN 10_1101-2021_01_08_425864 836 39 . . . 10_1101-2021_01_08_425864 837 1 It -PRON- PRP 10_1101-2021_01_08_425864 837 2 is be VBZ 10_1101-2021_01_08_425864 837 3 The the DT 10_1101-2021_01_08_425864 837 4 copyright copyright NN 10_1101-2021_01_08_425864 837 5 holder holder NN 10_1101-2021_01_08_425864 837 6 for for IN 10_1101-2021_01_08_425864 837 7 this this DT 10_1101-2021_01_08_425864 837 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 837 9 version version NN 10_1101-2021_01_08_425864 837 10 posted post VBD 10_1101-2021_01_08_425864 837 11 January January NNP 10_1101-2021_01_08_425864 837 12 8 8 CD 10_1101-2021_01_08_425864 837 13 , , , 10_1101-2021_01_08_425864 837 14 2021 2021 CD 10_1101-2021_01_08_425864 837 15 . . . 10_1101-2021_01_08_425864 837 16 ; ; : 10_1101-2021_01_08_425864 837 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 837 18 : : : 10_1101-2021_01_08_425864 837 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 837 20 preprint preprint NN 10_1101-2021_01_08_425864 837 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 NN 10_1101-2021_01_08_425864 837 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 837 23 891 891 CD 10_1101-2021_01_08_425864 837 24 Supporting support VBG 10_1101-2021_01_08_425864 837 25 information information NN 10_1101-2021_01_08_425864 837 26 892 892 CD 10_1101-2021_01_08_425864 837 27 S1 s1 NN 10_1101-2021_01_08_425864 837 28 893 893 CD 10_1101-2021_01_08_425864 837 29 S1 S1 NNS 10_1101-2021_01_08_425864 837 30 Fig fig NN 10_1101-2021_01_08_425864 837 31 . . . 10_1101-2021_01_08_425864 838 1 1H 1h LS 10_1101-2021_01_08_425864 838 2 - - : 10_1101-2021_01_08_425864 838 3 NMR NMR NNP 10_1101-2021_01_08_425864 838 4 analysis analysis NN 10_1101-2021_01_08_425864 838 5 of of IN 10_1101-2021_01_08_425864 838 6 excreted excreted JJ 10_1101-2021_01_08_425864 838 7 end end NN 10_1101-2021_01_08_425864 838 8 products product NNS 10_1101-2021_01_08_425864 838 9 from from IN 10_1101-2021_01_08_425864 838 10 glucose glucose NN 10_1101-2021_01_08_425864 838 11 and and CC 10_1101-2021_01_08_425864 838 12 threonine threonine NN 10_1101-2021_01_08_425864 838 13 metabolism metabolism NN 10_1101-2021_01_08_425864 838 14 . . . 10_1101-2021_01_08_425864 839 1 The the DT 10_1101-2021_01_08_425864 839 2 894 894 CD 10_1101-2021_01_08_425864 839 3 metabolic metabolic JJ 10_1101-2021_01_08_425864 839 4 end end NN 10_1101-2021_01_08_425864 839 5 products product NNS 10_1101-2021_01_08_425864 839 6 ( ( -LRB- 10_1101-2021_01_08_425864 839 7 succinate succinate NNP 10_1101-2021_01_08_425864 839 8 , , , 10_1101-2021_01_08_425864 839 9 acetate acetate JJ 10_1101-2021_01_08_425864 839 10 , , , 10_1101-2021_01_08_425864 839 11 alanine alanine NN 10_1101-2021_01_08_425864 839 12 and and CC 10_1101-2021_01_08_425864 839 13 lactate lactate NN 10_1101-2021_01_08_425864 839 14 ) ) -RRB- 10_1101-2021_01_08_425864 839 15 excreted excrete VBN 10_1101-2021_01_08_425864 839 16 by by IN 10_1101-2021_01_08_425864 839 17 the the DT 10_1101-2021_01_08_425864 839 18 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 839 19 cells cell NNS 10_1101-2021_01_08_425864 839 20 that that IN 10_1101-2021_01_08_425864 839 21 895 895 CD 10_1101-2021_01_08_425864 839 22 were be VBD 10_1101-2021_01_08_425864 839 23 incubated incubate VBN 10_1101-2021_01_08_425864 839 24 after after IN 10_1101-2021_01_08_425864 839 25 6 6 CD 10_1101-2021_01_08_425864 839 26 h h NN 10_1101-2021_01_08_425864 839 27 in in IN 10_1101-2021_01_08_425864 839 28 PBS PBS NNP 10_1101-2021_01_08_425864 839 29 ( ( -LRB- 10_1101-2021_01_08_425864 839 30 A A NNP 10_1101-2021_01_08_425864 839 31 ) ) -RRB- 10_1101-2021_01_08_425864 839 32 , , , 10_1101-2021_01_08_425864 839 33 PBS PBS NNP 10_1101-2021_01_08_425864 839 34 after after IN 10_1101-2021_01_08_425864 839 35 16 16 CD 10_1101-2021_01_08_425864 839 36 h h NN 10_1101-2021_01_08_425864 839 37 of of IN 10_1101-2021_01_08_425864 839 38 starvation starvation NN 10_1101-2021_01_08_425864 839 39 without without IN 10_1101-2021_01_08_425864 839 40 ( ( -LRB- 10_1101-2021_01_08_425864 839 41 B b NN 10_1101-2021_01_08_425864 839 42 ) ) -RRB- 10_1101-2021_01_08_425864 839 43 or or CC 10_1101-2021_01_08_425864 839 44 with with IN 10_1101-2021_01_08_425864 839 45 D-[U-13C]- D-[U-13C]- NNP 10_1101-2021_01_08_425864 839 46 896 896 CD 10_1101-2021_01_08_425864 839 47 glucose glucose NN 10_1101-2021_01_08_425864 839 48 ( ( -LRB- 10_1101-2021_01_08_425864 839 49 C c NN 10_1101-2021_01_08_425864 839 50 ) ) -RRB- 10_1101-2021_01_08_425864 839 51 or or CC 10_1101-2021_01_08_425864 839 52 palmitate palmitate NN 10_1101-2021_01_08_425864 839 53 ( ( -LRB- 10_1101-2021_01_08_425864 839 54 D d NN 10_1101-2021_01_08_425864 839 55 ) ) -RRB- 10_1101-2021_01_08_425864 839 56 were be VBD 10_1101-2021_01_08_425864 839 57 determined determine VBN 10_1101-2021_01_08_425864 839 58 by by IN 10_1101-2021_01_08_425864 839 59 1H 1h JJ 10_1101-2021_01_08_425864 839 60 - - HYPH 10_1101-2021_01_08_425864 839 61 NMR NMR NNP 10_1101-2021_01_08_425864 839 62 . . . 10_1101-2021_01_08_425864 840 1 Each each DT 10_1101-2021_01_08_425864 840 2 spectrum spectrum NN 10_1101-2021_01_08_425864 840 3 corresponds correspond VBZ 10_1101-2021_01_08_425864 840 4 to to IN 10_1101-2021_01_08_425864 840 5 one one CD 10_1101-2021_01_08_425864 840 6 897 897 CD 10_1101-2021_01_08_425864 840 7 representative representative JJ 10_1101-2021_01_08_425864 840 8 experiment experiment NN 10_1101-2021_01_08_425864 840 9 from from IN 10_1101-2021_01_08_425864 840 10 a a DT 10_1101-2021_01_08_425864 840 11 set set NN 10_1101-2021_01_08_425864 840 12 of of IN 10_1101-2021_01_08_425864 840 13 at at IN 10_1101-2021_01_08_425864 840 14 least least JJS 10_1101-2021_01_08_425864 840 15 3 3 CD 10_1101-2021_01_08_425864 840 16 . . . 10_1101-2021_01_08_425864 841 1 A a DT 10_1101-2021_01_08_425864 841 2 part part NN 10_1101-2021_01_08_425864 841 3 of of IN 10_1101-2021_01_08_425864 841 4 each each DT 10_1101-2021_01_08_425864 841 5 spectrum spectrum NN 10_1101-2021_01_08_425864 841 6 ranging range VBG 10_1101-2021_01_08_425864 841 7 from from IN 10_1101-2021_01_08_425864 841 8 0.5 0.5 CD 10_1101-2021_01_08_425864 841 9 ppm ppm NN 10_1101-2021_01_08_425864 841 10 to to IN 10_1101-2021_01_08_425864 841 11 898 898 CD 10_1101-2021_01_08_425864 841 12 4 4 CD 10_1101-2021_01_08_425864 841 13 ppm ppm NN 10_1101-2021_01_08_425864 841 14 is be VBZ 10_1101-2021_01_08_425864 841 15 shown show VBN 10_1101-2021_01_08_425864 841 16 . . . 10_1101-2021_01_08_425864 842 1 The the DT 10_1101-2021_01_08_425864 842 2 resonances resonance NNS 10_1101-2021_01_08_425864 842 3 were be VBD 10_1101-2021_01_08_425864 842 4 assigned assign VBN 10_1101-2021_01_08_425864 842 5 as as IN 10_1101-2021_01_08_425864 842 6 indicated indicate VBN 10_1101-2021_01_08_425864 842 7 : : : 10_1101-2021_01_08_425864 842 8 A12 A12 NNP 10_1101-2021_01_08_425864 842 9 , , , 10_1101-2021_01_08_425864 842 10 acetate acetate JJ 10_1101-2021_01_08_425864 842 11 ; ; : 10_1101-2021_01_08_425864 842 12 A13 a13 JJ 10_1101-2021_01_08_425864 842 13 , , , 10_1101-2021_01_08_425864 842 14 13C 13C NNP 10_1101-2021_01_08_425864 842 15 - - HYPH 10_1101-2021_01_08_425864 842 16 enriched enrich VBN 10_1101-2021_01_08_425864 842 17 acetate acetate NN 10_1101-2021_01_08_425864 842 18 ; ; : 10_1101-2021_01_08_425864 842 19 899 899 CD 10_1101-2021_01_08_425864 842 20 Al12 Al12 NNP 10_1101-2021_01_08_425864 842 21 , , , 10_1101-2021_01_08_425864 842 22 alanine alanine NN 10_1101-2021_01_08_425864 842 23 ; ; : 10_1101-2021_01_08_425864 842 24 Al13 Al13 NNP 10_1101-2021_01_08_425864 842 25 , , , 10_1101-2021_01_08_425864 842 26 13C 13C NNP 10_1101-2021_01_08_425864 842 27 - - HYPH 10_1101-2021_01_08_425864 842 28 enriched enrich VBN 10_1101-2021_01_08_425864 842 29 alanine alanine NN 10_1101-2021_01_08_425864 842 30 ; ; : 10_1101-2021_01_08_425864 842 31 G13 G13 NNP 10_1101-2021_01_08_425864 842 32 , , , 10_1101-2021_01_08_425864 842 33 13C 13c CD 10_1101-2021_01_08_425864 842 34 - - HYPH 10_1101-2021_01_08_425864 842 35 enriched enrich VBN 10_1101-2021_01_08_425864 842 36 glucose glucose NN 10_1101-2021_01_08_425864 842 37 ; ; : 10_1101-2021_01_08_425864 842 38 L12 L12 NNP 10_1101-2021_01_08_425864 842 39 , , , 10_1101-2021_01_08_425864 842 40 lactate lactate NN 10_1101-2021_01_08_425864 842 41 ; ; : 10_1101-2021_01_08_425864 842 42 L13 L13 NNP 10_1101-2021_01_08_425864 842 43 , , , 10_1101-2021_01_08_425864 842 44 13C 13C NNP 10_1101-2021_01_08_425864 842 45 - - HYPH 10_1101-2021_01_08_425864 842 46 enriched enrich VBN 10_1101-2021_01_08_425864 842 47 900 900 CD 10_1101-2021_01_08_425864 842 48 lactate lactate NN 10_1101-2021_01_08_425864 842 49 ; ; : 10_1101-2021_01_08_425864 842 50 P12 P12 NNP 10_1101-2021_01_08_425864 842 51 , , , 10_1101-2021_01_08_425864 842 52 palmitate palmitate NN 10_1101-2021_01_08_425864 842 53 ; ; : 10_1101-2021_01_08_425864 842 54 S12 S12 NNP 10_1101-2021_01_08_425864 842 55 , , , 10_1101-2021_01_08_425864 842 56 succinate succinate NN 10_1101-2021_01_08_425864 842 57 ; ; : 10_1101-2021_01_08_425864 842 58 and and CC 10_1101-2021_01_08_425864 842 59 S13 S13 NNP 10_1101-2021_01_08_425864 842 60 , , , 10_1101-2021_01_08_425864 842 61 13C 13C NNP 10_1101-2021_01_08_425864 842 62 - - HYPH 10_1101-2021_01_08_425864 842 63 enriched enrich VBN 10_1101-2021_01_08_425864 842 64 succinate succinate NN 10_1101-2021_01_08_425864 842 65 . . . 10_1101-2021_01_08_425864 843 1 901 901 CD 10_1101-2021_01_08_425864 843 2 902 902 CD 10_1101-2021_01_08_425864 843 3 S2 s2 NN 10_1101-2021_01_08_425864 843 4 903 903 CD 10_1101-2021_01_08_425864 843 5 904 904 CD 10_1101-2021_01_08_425864 843 6 S2 s2 NN 10_1101-2021_01_08_425864 843 7 Fig fig NN 10_1101-2021_01_08_425864 843 8 . . . 10_1101-2021_01_08_425864 844 1 Time time NN 10_1101-2021_01_08_425864 844 2 course course NN 10_1101-2021_01_08_425864 844 3 activities activity NNS 10_1101-2021_01_08_425864 844 4 of of IN 10_1101-2021_01_08_425864 844 5 enzymes enzyme NNS 10_1101-2021_01_08_425864 844 6 measured measure VBN 10_1101-2021_01_08_425864 844 7 in in IN 10_1101-2021_01_08_425864 844 8 this this DT 10_1101-2021_01_08_425864 844 9 work work NN 10_1101-2021_01_08_425864 844 10 . . . 10_1101-2021_01_08_425864 845 1 A a LS 10_1101-2021_01_08_425864 845 2 ) ) -RRB- 10_1101-2021_01_08_425864 845 3 ( ( -LRB- 10_1101-2021_01_08_425864 845 4 ACC ACC NNP 10_1101-2021_01_08_425864 845 5 ) ) -RRB- 10_1101-2021_01_08_425864 845 6 acetyl acetyl NN 10_1101-2021_01_08_425864 845 7 - - HYPH 10_1101-2021_01_08_425864 845 8 CoA CoA NNP 10_1101-2021_01_08_425864 845 9 905 905 CD 10_1101-2021_01_08_425864 845 10 carboxylase carboxylase NN 10_1101-2021_01_08_425864 845 11 , , , 10_1101-2021_01_08_425864 845 12 B b NN 10_1101-2021_01_08_425864 845 13 ) ) -RRB- 10_1101-2021_01_08_425864 845 14 ( ( -LRB- 10_1101-2021_01_08_425864 845 15 CPT1 CPT1 NNP 10_1101-2021_01_08_425864 845 16 ) ) -RRB- 10_1101-2021_01_08_425864 845 17 carnitine carnitine NN 10_1101-2021_01_08_425864 845 18 - - HYPH 10_1101-2021_01_08_425864 845 19 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 845 20 , , , 10_1101-2021_01_08_425864 845 21 and and CC 10_1101-2021_01_08_425864 845 22 C C NNP 10_1101-2021_01_08_425864 845 23 ) ) -RRB- 10_1101-2021_01_08_425864 845 24 ( ( -LRB- 10_1101-2021_01_08_425864 845 25 SPT SPT NNP 10_1101-2021_01_08_425864 845 26 ) ) -RRB- 10_1101-2021_01_08_425864 845 27 serine serine JJ 10_1101-2021_01_08_425864 845 28 palmitoyltransferase palmitoyltransferase NN 10_1101-2021_01_08_425864 845 29 . . . 10_1101-2021_01_08_425864 846 1 All all DT 10_1101-2021_01_08_425864 846 2 906 906 CD 10_1101-2021_01_08_425864 846 3 the the DT 10_1101-2021_01_08_425864 846 4 activities activity NNS 10_1101-2021_01_08_425864 846 5 were be VBD 10_1101-2021_01_08_425864 846 6 measured measure VBN 10_1101-2021_01_08_425864 846 7 in in IN 10_1101-2021_01_08_425864 846 8 cell cell NN 10_1101-2021_01_08_425864 846 9 - - HYPH 10_1101-2021_01_08_425864 846 10 free free JJ 10_1101-2021_01_08_425864 846 11 extracts extract NNS 10_1101-2021_01_08_425864 846 12 of of IN 10_1101-2021_01_08_425864 846 13 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 846 14 forms form NNS 10_1101-2021_01_08_425864 846 15 at at IN 10_1101-2021_01_08_425864 846 16 different different JJ 10_1101-2021_01_08_425864 846 17 moments moment NNS 10_1101-2021_01_08_425864 846 18 of of IN 10_1101-2021_01_08_425864 846 19 the the DT 10_1101-2021_01_08_425864 846 20 907 907 CD 10_1101-2021_01_08_425864 846 21 growth growth NN 10_1101-2021_01_08_425864 846 22 curve curve NN 10_1101-2021_01_08_425864 846 23 as as IN 10_1101-2021_01_08_425864 846 24 indicated indicate VBN 10_1101-2021_01_08_425864 846 25 in in IN 10_1101-2021_01_08_425864 846 26 the the DT 10_1101-2021_01_08_425864 846 27 main main JJ 10_1101-2021_01_08_425864 846 28 text text NN 10_1101-2021_01_08_425864 846 29 . . . 10_1101-2021_01_08_425864 847 1 All all PDT 10_1101-2021_01_08_425864 847 2 the the DT 10_1101-2021_01_08_425864 847 3 measurements measurement NNS 10_1101-2021_01_08_425864 847 4 were be VBD 10_1101-2021_01_08_425864 847 5 performed perform VBN 10_1101-2021_01_08_425864 847 6 in in IN 10_1101-2021_01_08_425864 847 7 triplicates triplicate NNS 10_1101-2021_01_08_425864 847 8 . . . 10_1101-2021_01_08_425864 848 1 908 908 CD 10_1101-2021_01_08_425864 848 2 S3 S3 NNP 10_1101-2021_01_08_425864 848 3 909 909 CD 10_1101-2021_01_08_425864 848 4 910 910 CD 10_1101-2021_01_08_425864 848 5 To to TO 10_1101-2021_01_08_425864 848 6 check check VB 10_1101-2021_01_08_425864 848 7 if if IN 10_1101-2021_01_08_425864 848 8 other other JJ 10_1101-2021_01_08_425864 848 9 well well RB 10_1101-2021_01_08_425864 848 10 - - HYPH 10_1101-2021_01_08_425864 848 11 known know VBN 10_1101-2021_01_08_425864 848 12 FAO FAO NNP 10_1101-2021_01_08_425864 848 13 inhibitors inhibitor NNS 10_1101-2021_01_08_425864 848 14 have have VBP 10_1101-2021_01_08_425864 848 15 the the DT 10_1101-2021_01_08_425864 848 16 same same JJ 10_1101-2021_01_08_425864 848 17 effect effect NN 10_1101-2021_01_08_425864 848 18 on on IN 10_1101-2021_01_08_425864 848 19 the the DT 10_1101-2021_01_08_425864 848 20 proliferation proliferation NN 10_1101-2021_01_08_425864 848 21 of of IN 10_1101-2021_01_08_425864 848 22 T. T. NNP 10_1101-2021_01_08_425864 848 23 cruzi cruzi NNS 10_1101-2021_01_08_425864 848 24 911 911 CD 10_1101-2021_01_08_425864 848 25 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 848 26 , , , 10_1101-2021_01_08_425864 848 27 we -PRON- PRP 10_1101-2021_01_08_425864 848 28 performed perform VBD 10_1101-2021_01_08_425864 848 29 the the DT 10_1101-2021_01_08_425864 848 30 same same JJ 10_1101-2021_01_08_425864 848 31 assay assay NN 10_1101-2021_01_08_425864 848 32 as as IN 10_1101-2021_01_08_425864 848 33 described describe VBN 10_1101-2021_01_08_425864 848 34 in in IN 10_1101-2021_01_08_425864 848 35 Materials Materials NNP 10_1101-2021_01_08_425864 848 36 and and CC 10_1101-2021_01_08_425864 848 37 Methods method NNS 10_1101-2021_01_08_425864 848 38 by by IN 10_1101-2021_01_08_425864 848 39 evaluating evaluate VBG 10_1101-2021_01_08_425864 848 40 912 912 CD 10_1101-2021_01_08_425864 848 41 different different JJ 10_1101-2021_01_08_425864 848 42 concentrations concentration NNS 10_1101-2021_01_08_425864 848 43 of of IN 10_1101-2021_01_08_425864 848 44 valproic valproic JJ 10_1101-2021_01_08_425864 848 45 acid acid NN 10_1101-2021_01_08_425864 848 46 ( ( -LRB- 10_1101-2021_01_08_425864 848 47 AV av NN 10_1101-2021_01_08_425864 848 48 ) ) -RRB- 10_1101-2021_01_08_425864 848 49 [ [ -LRB- 10_1101-2021_01_08_425864 848 50 57 57 CD 10_1101-2021_01_08_425864 848 51 ] ] -RRB- 10_1101-2021_01_08_425864 848 52 , , , 10_1101-2021_01_08_425864 848 53 trimetazidine trimetazidine VB 10_1101-2021_01_08_425864 848 54 [ [ -LRB- 10_1101-2021_01_08_425864 848 55 58,59 58,59 CD 10_1101-2021_01_08_425864 848 56 ] ] -RRB- 10_1101-2021_01_08_425864 848 57 and and CC 10_1101-2021_01_08_425864 848 58 β β NN 10_1101-2021_01_08_425864 848 59 - - HYPH 10_1101-2021_01_08_425864 848 60 hydroxybutyrate hydroxybutyrate NN 10_1101-2021_01_08_425864 848 61 [ [ -LRB- 10_1101-2021_01_08_425864 848 62 60 60 CD 10_1101-2021_01_08_425864 848 63 ] ] -RRB- 10_1101-2021_01_08_425864 848 64 , , , 10_1101-2021_01_08_425864 848 65 913 913 CD 10_1101-2021_01_08_425864 848 66 which which WDT 10_1101-2021_01_08_425864 848 67 are be VBP 10_1101-2021_01_08_425864 848 68 inhibitors inhibitor NNS 10_1101-2021_01_08_425864 848 69 of of IN 10_1101-2021_01_08_425864 848 70 3-ketothiolase 3-ketothiolase CD 10_1101-2021_01_08_425864 848 71 . . . 10_1101-2021_01_08_425864 849 1 Because because IN 10_1101-2021_01_08_425864 849 2 they -PRON- PRP 10_1101-2021_01_08_425864 849 3 did do VBD 10_1101-2021_01_08_425864 849 4 not not RB 10_1101-2021_01_08_425864 849 5 affect affect VB 10_1101-2021_01_08_425864 849 6 the the DT 10_1101-2021_01_08_425864 849 7 proliferation proliferation NN 10_1101-2021_01_08_425864 849 8 of of IN 10_1101-2021_01_08_425864 849 9 the the DT 10_1101-2021_01_08_425864 849 10 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 849 11 914 914 CD 10_1101-2021_01_08_425864 849 12 forms form NNS 10_1101-2021_01_08_425864 849 13 , , , 10_1101-2021_01_08_425864 849 14 we -PRON- PRP 10_1101-2021_01_08_425864 849 15 used use VBD 10_1101-2021_01_08_425864 849 16 the the DT 10_1101-2021_01_08_425864 849 17 higher high JJR 10_1101-2021_01_08_425864 849 18 concentration concentration NN 10_1101-2021_01_08_425864 849 19 evaluated evaluate VBN 10_1101-2021_01_08_425864 849 20 in in IN 10_1101-2021_01_08_425864 849 21 these these DT 10_1101-2021_01_08_425864 849 22 assays assay NNS 10_1101-2021_01_08_425864 849 23 to to TO 10_1101-2021_01_08_425864 849 24 know know VB 10_1101-2021_01_08_425864 849 25 if if IN 10_1101-2021_01_08_425864 849 26 the the DT 10_1101-2021_01_08_425864 849 27 compounds compound NNS 10_1101-2021_01_08_425864 849 28 inhibit inhibit VBP 10_1101-2021_01_08_425864 849 29 915 915 CD 10_1101-2021_01_08_425864 849 30 FAO FAO NNP 10_1101-2021_01_08_425864 849 31 by by IN 10_1101-2021_01_08_425864 849 32 14CO2 14co2 CD 10_1101-2021_01_08_425864 849 33 trapping trapping NN 10_1101-2021_01_08_425864 849 34 by by IN 10_1101-2021_01_08_425864 849 35 using use VBG 10_1101-2021_01_08_425864 849 36 U-14C u-14c NN 10_1101-2021_01_08_425864 849 37 - - HYPH 10_1101-2021_01_08_425864 849 38 palmitate palmitate NN 10_1101-2021_01_08_425864 849 39 as as IN 10_1101-2021_01_08_425864 849 40 a a DT 10_1101-2021_01_08_425864 849 41 substrate substrate NN 10_1101-2021_01_08_425864 849 42 . . . 10_1101-2021_01_08_425864 850 1 As as IN 10_1101-2021_01_08_425864 850 2 observed observe VBN 10_1101-2021_01_08_425864 850 3 , , , 10_1101-2021_01_08_425864 850 4 none none NN 10_1101-2021_01_08_425864 850 5 of of IN 10_1101-2021_01_08_425864 850 6 these these DT 10_1101-2021_01_08_425864 850 7 916 916 CD 10_1101-2021_01_08_425864 850 8 compound compound NN 10_1101-2021_01_08_425864 850 9 inhibited inhibit VBD 10_1101-2021_01_08_425864 850 10 the the DT 10_1101-2021_01_08_425864 850 11 14CO2 14co2 CD 10_1101-2021_01_08_425864 850 12 production production NN 10_1101-2021_01_08_425864 850 13 from from IN 10_1101-2021_01_08_425864 850 14 palmitate palmitate NN 10_1101-2021_01_08_425864 850 15 , , , 10_1101-2021_01_08_425864 850 16 confirming confirm VBG 10_1101-2021_01_08_425864 850 17 that that IN 10_1101-2021_01_08_425864 850 18 they -PRON- PRP 10_1101-2021_01_08_425864 850 19 are be VBP 10_1101-2021_01_08_425864 850 20 not not RB 10_1101-2021_01_08_425864 850 21 inhibiting inhibit VBG 10_1101-2021_01_08_425864 850 22 FAO FAO NNP 10_1101-2021_01_08_425864 850 23 917 917 CD 10_1101-2021_01_08_425864 850 24 in in IN 10_1101-2021_01_08_425864 850 25 T. t. NN 10_1101-2021_01_08_425864 850 26 cruzi cruzi NNS 10_1101-2021_01_08_425864 850 27 . . . 10_1101-2021_01_08_425864 851 1 918 918 CD 10_1101-2021_01_08_425864 851 2 919 919 CD 10_1101-2021_01_08_425864 851 3 S3 S3 NNP 10_1101-2021_01_08_425864 851 4 Fig Fig NNP 10_1101-2021_01_08_425864 851 5 . . . 10_1101-2021_01_08_425864 852 1 Other other JJ 10_1101-2021_01_08_425864 852 2 FAO FAO NNP 10_1101-2021_01_08_425864 852 3 inhibitors inhibitor NNS 10_1101-2021_01_08_425864 852 4 did do VBD 10_1101-2021_01_08_425864 852 5 not not RB 10_1101-2021_01_08_425864 852 6 affect affect VB 10_1101-2021_01_08_425864 852 7 cell cell NN 10_1101-2021_01_08_425864 852 8 proliferation proliferation NN 10_1101-2021_01_08_425864 852 9 and and CC 10_1101-2021_01_08_425864 852 10 FAO FAO NNP 10_1101-2021_01_08_425864 852 11 in in IN 10_1101-2021_01_08_425864 852 12 the the DT 10_1101-2021_01_08_425864 852 13 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 852 14 920 920 CD 10_1101-2021_01_08_425864 852 15 forms form NNS 10_1101-2021_01_08_425864 852 16 . . . 10_1101-2021_01_08_425864 853 1 The the DT 10_1101-2021_01_08_425864 853 2 compounds compound NNS 10_1101-2021_01_08_425864 853 3 were be VBD 10_1101-2021_01_08_425864 853 4 evaluated evaluate VBN 10_1101-2021_01_08_425864 853 5 at at IN 10_1101-2021_01_08_425864 853 6 concentrations concentration NNS 10_1101-2021_01_08_425864 853 7 between between IN 10_1101-2021_01_08_425864 853 8 0.1 0.1 CD 10_1101-2021_01_08_425864 853 9 and and CC 10_1101-2021_01_08_425864 853 10 1000 1000 CD 10_1101-2021_01_08_425864 853 11 µM. µM. NNP 10_1101-2021_01_08_425864 854 1 For for IN 10_1101-2021_01_08_425864 854 2 positive positive JJ 10_1101-2021_01_08_425864 854 3 921 921 CD 10_1101-2021_01_08_425864 854 4 controls control NNS 10_1101-2021_01_08_425864 854 5 of of IN 10_1101-2021_01_08_425864 854 6 dead dead JJ 10_1101-2021_01_08_425864 854 7 cells cell NNS 10_1101-2021_01_08_425864 854 8 , , , 10_1101-2021_01_08_425864 854 9 a a DT 10_1101-2021_01_08_425864 854 10 combination combination NN 10_1101-2021_01_08_425864 854 11 of of IN 10_1101-2021_01_08_425864 854 12 antimycin antimycin NNS 10_1101-2021_01_08_425864 854 13 ( ( -LRB- 10_1101-2021_01_08_425864 854 14 0.5 0.5 CD 10_1101-2021_01_08_425864 854 15 µM µM NNP 10_1101-2021_01_08_425864 854 16 ) ) -RRB- 10_1101-2021_01_08_425864 854 17 and and CC 10_1101-2021_01_08_425864 854 18 rotenone rotenone NN 10_1101-2021_01_08_425864 854 19 ( ( -LRB- 10_1101-2021_01_08_425864 854 20 60 60 CD 10_1101-2021_01_08_425864 854 21 µM µM NNP 10_1101-2021_01_08_425864 854 22 ) ) -RRB- 10_1101-2021_01_08_425864 854 23 were be VBD 10_1101-2021_01_08_425864 854 24 used use VBN 10_1101-2021_01_08_425864 854 25 . . . 10_1101-2021_01_08_425864 855 1 The the DT 10_1101-2021_01_08_425864 855 2 922 922 CD 10_1101-2021_01_08_425864 855 3 maximum maximum JJ 10_1101-2021_01_08_425864 855 4 concentration concentration NN 10_1101-2021_01_08_425864 855 5 tested test VBN 10_1101-2021_01_08_425864 855 6 for for IN 10_1101-2021_01_08_425864 855 7 these these DT 10_1101-2021_01_08_425864 855 8 compounds compound NNS 10_1101-2021_01_08_425864 855 9 does do VBZ 10_1101-2021_01_08_425864 855 10 not not RB 10_1101-2021_01_08_425864 855 11 diminish diminish VB 10_1101-2021_01_08_425864 855 12 CO2 CO2 NNP 10_1101-2021_01_08_425864 855 13 liberation liberation NN 10_1101-2021_01_08_425864 855 14 from from IN 10_1101-2021_01_08_425864 855 15 FAO FAO NNP 10_1101-2021_01_08_425864 855 16 . . . 10_1101-2021_01_08_425864 856 1 A a DT 10_1101-2021_01_08_425864 856 2 ) ) -RRB- 10_1101-2021_01_08_425864 856 3 923 923 CD 10_1101-2021_01_08_425864 856 4 Valproic valproic JJ 10_1101-2021_01_08_425864 856 5 Acid acid NN 10_1101-2021_01_08_425864 856 6 ( ( -LRB- 10_1101-2021_01_08_425864 856 7 AV av NN 10_1101-2021_01_08_425864 856 8 ) ) -RRB- 10_1101-2021_01_08_425864 856 9 . . . 10_1101-2021_01_08_425864 857 1 B b NN 10_1101-2021_01_08_425864 857 2 ) ) -RRB- 10_1101-2021_01_08_425864 857 3 Trimetazidine Trimetazidine NNP 10_1101-2021_01_08_425864 857 4 ( ( -LRB- 10_1101-2021_01_08_425864 857 5 TMZ TMZ NNP 10_1101-2021_01_08_425864 857 6 ) ) -RRB- 10_1101-2021_01_08_425864 857 7 . . . 10_1101-2021_01_08_425864 858 1 C C NNP 10_1101-2021_01_08_425864 858 2 ) ) -RRB- 10_1101-2021_01_08_425864 858 3 β β NN 10_1101-2021_01_08_425864 858 4 - - HYPH 10_1101-2021_01_08_425864 858 5 hydroxybutyrate hydroxybutyrate NN 10_1101-2021_01_08_425864 858 6 ( ( -LRB- 10_1101-2021_01_08_425864 858 7 βHOB βhob NN 10_1101-2021_01_08_425864 858 8 ) ) -RRB- 10_1101-2021_01_08_425864 858 9 . . . 10_1101-2021_01_08_425864 859 1 924 924 CD 10_1101-2021_01_08_425864 859 2 925 925 CD 10_1101-2021_01_08_425864 859 3 S4 s4 NN 10_1101-2021_01_08_425864 859 4 926 926 CD 10_1101-2021_01_08_425864 859 5 927 927 CD 10_1101-2021_01_08_425864 859 6 In in IN 10_1101-2021_01_08_425864 859 7 this this DT 10_1101-2021_01_08_425864 859 8 study study NN 10_1101-2021_01_08_425864 859 9 , , , 10_1101-2021_01_08_425864 859 10 we -PRON- PRP 10_1101-2021_01_08_425864 859 11 showed show VBD 10_1101-2021_01_08_425864 859 12 that that IN 10_1101-2021_01_08_425864 859 13 the the DT 10_1101-2021_01_08_425864 859 14 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 859 15 forms form NNS 10_1101-2021_01_08_425864 859 16 of of IN 10_1101-2021_01_08_425864 859 17 T. T. NNP 10_1101-2021_01_08_425864 859 18 cruzi cruzi NNS 10_1101-2021_01_08_425864 859 19 present present JJ 10_1101-2021_01_08_425864 859 20 low low JJ 10_1101-2021_01_08_425864 859 21 sensitivity sensitivity NN 10_1101-2021_01_08_425864 859 22 in in IN 10_1101-2021_01_08_425864 859 23 response response NN 10_1101-2021_01_08_425864 859 24 928 928 CD 10_1101-2021_01_08_425864 859 25 to to TO 10_1101-2021_01_08_425864 859 26 ETO eto VB 10_1101-2021_01_08_425864 859 27 treatment treatment NN 10_1101-2021_01_08_425864 859 28 . . . 10_1101-2021_01_08_425864 860 1 Recently recently RB 10_1101-2021_01_08_425864 860 2 , , , 10_1101-2021_01_08_425864 860 3 some some DT 10_1101-2021_01_08_425864 860 4 groups group NNS 10_1101-2021_01_08_425864 860 5 described describe VBD 10_1101-2021_01_08_425864 860 6 off off IN 10_1101-2021_01_08_425864 860 7 - - HYPH 10_1101-2021_01_08_425864 860 8 target target NN 10_1101-2021_01_08_425864 860 9 effects effect NNS 10_1101-2021_01_08_425864 860 10 when when WRB 10_1101-2021_01_08_425864 860 11 ETO ETO NNP 10_1101-2021_01_08_425864 860 12 is be VBZ 10_1101-2021_01_08_425864 860 13 used use VBN 10_1101-2021_01_08_425864 860 14 at at IN 10_1101-2021_01_08_425864 860 15 929 929 CD 10_1101-2021_01_08_425864 860 16 concentrations concentration NNS 10_1101-2021_01_08_425864 860 17 of of IN 10_1101-2021_01_08_425864 860 18 up up IN 10_1101-2021_01_08_425864 860 19 to to TO 10_1101-2021_01_08_425864 860 20 200 200 CD 10_1101-2021_01_08_425864 860 21 μM μM NNP 10_1101-2021_01_08_425864 860 22 [ [ -LRB- 10_1101-2021_01_08_425864 860 23 61,62 61,62 CD 10_1101-2021_01_08_425864 860 24 ] ] -RRB- 10_1101-2021_01_08_425864 860 25 . . . 10_1101-2021_01_08_425864 861 1 To to TO 10_1101-2021_01_08_425864 861 2 validate validate VB 10_1101-2021_01_08_425864 861 3 ETO ETO NNP 10_1101-2021_01_08_425864 861 4 as as IN 10_1101-2021_01_08_425864 861 5 an an DT 10_1101-2021_01_08_425864 861 6 FAO FAO NNP 10_1101-2021_01_08_425864 861 7 inhibitor inhibitor NN 10_1101-2021_01_08_425864 861 8 of of IN 10_1101-2021_01_08_425864 861 9 T. T. NNP 10_1101-2021_01_08_425864 861 10 cruzi cruzi NNS 10_1101-2021_01_08_425864 861 11 , , , 10_1101-2021_01_08_425864 861 12 the the DT 10_1101-2021_01_08_425864 861 13 parasites parasite NNS 10_1101-2021_01_08_425864 861 14 930 930 CD 10_1101-2021_01_08_425864 861 15 were be VBD 10_1101-2021_01_08_425864 861 16 incubated incubate VBN 10_1101-2021_01_08_425864 861 17 for for IN 10_1101-2021_01_08_425864 861 18 24 24 CD 10_1101-2021_01_08_425864 861 19 h h NN 10_1101-2021_01_08_425864 861 20 in in IN 10_1101-2021_01_08_425864 861 21 PBS PBS NNP 10_1101-2021_01_08_425864 861 22 ( ( -LRB- 10_1101-2021_01_08_425864 861 23 negative negative JJ 10_1101-2021_01_08_425864 861 24 control control NN 10_1101-2021_01_08_425864 861 25 ) ) -RRB- 10_1101-2021_01_08_425864 861 26 , , , 10_1101-2021_01_08_425864 861 27 0.1 0.1 CD 10_1101-2021_01_08_425864 861 28 mM mm NN 10_1101-2021_01_08_425864 861 29 palmitate palmitate NN 10_1101-2021_01_08_425864 861 30 supplemented supplement VBN 10_1101-2021_01_08_425864 861 31 with with IN 10_1101-2021_01_08_425864 861 32 BSA BSA NNP 10_1101-2021_01_08_425864 861 33 , , , 10_1101-2021_01_08_425864 861 34 5.0 5.0 CD 10_1101-2021_01_08_425864 861 35 .CC .CC NFP 10_1101-2021_01_08_425864 861 36 - - : 10_1101-2021_01_08_425864 861 37 BY by IN 10_1101-2021_01_08_425864 861 38 4.0 4.0 CD 10_1101-2021_01_08_425864 861 39 International international JJ 10_1101-2021_01_08_425864 861 40 licensemade licensemade NN 10_1101-2021_01_08_425864 861 41 available available JJ 10_1101-2021_01_08_425864 861 42 under under IN 10_1101-2021_01_08_425864 861 43 a a DT 10_1101-2021_01_08_425864 861 44 ( ( -LRB- 10_1101-2021_01_08_425864 861 45 which which WDT 10_1101-2021_01_08_425864 861 46 was be VBD 10_1101-2021_01_08_425864 861 47 not not RB 10_1101-2021_01_08_425864 861 48 certified certify VBN 10_1101-2021_01_08_425864 861 49 by by IN 10_1101-2021_01_08_425864 861 50 peer peer NN 10_1101-2021_01_08_425864 861 51 review review NN 10_1101-2021_01_08_425864 861 52 ) ) -RRB- 10_1101-2021_01_08_425864 861 53 is be VBZ 10_1101-2021_01_08_425864 861 54 the the DT 10_1101-2021_01_08_425864 861 55 author author NN 10_1101-2021_01_08_425864 861 56 / / SYM 10_1101-2021_01_08_425864 861 57 funder funder NN 10_1101-2021_01_08_425864 861 58 , , , 10_1101-2021_01_08_425864 861 59 who who WP 10_1101-2021_01_08_425864 861 60 has have VBZ 10_1101-2021_01_08_425864 861 61 granted grant VBN 10_1101-2021_01_08_425864 861 62 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 861 63 a a DT 10_1101-2021_01_08_425864 861 64 license license NN 10_1101-2021_01_08_425864 861 65 to to TO 10_1101-2021_01_08_425864 861 66 display display VB 10_1101-2021_01_08_425864 861 67 the the DT 10_1101-2021_01_08_425864 861 68 preprint preprint NN 10_1101-2021_01_08_425864 861 69 in in IN 10_1101-2021_01_08_425864 861 70 perpetuity perpetuity NN 10_1101-2021_01_08_425864 861 71 . . . 10_1101-2021_01_08_425864 862 1 It -PRON- PRP 10_1101-2021_01_08_425864 862 2 is be VBZ 10_1101-2021_01_08_425864 862 3 The the DT 10_1101-2021_01_08_425864 862 4 copyright copyright NN 10_1101-2021_01_08_425864 862 5 holder holder NN 10_1101-2021_01_08_425864 862 6 for for IN 10_1101-2021_01_08_425864 862 7 this this DT 10_1101-2021_01_08_425864 862 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 862 9 version version NN 10_1101-2021_01_08_425864 862 10 posted post VBD 10_1101-2021_01_08_425864 862 11 January January NNP 10_1101-2021_01_08_425864 862 12 8 8 CD 10_1101-2021_01_08_425864 862 13 , , , 10_1101-2021_01_08_425864 862 14 2021 2021 CD 10_1101-2021_01_08_425864 862 15 . . . 10_1101-2021_01_08_425864 862 16 ; ; : 10_1101-2021_01_08_425864 862 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 862 18 : : : 10_1101-2021_01_08_425864 862 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 862 20 preprint preprint NN 10_1101-2021_01_08_425864 862 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 862 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 862 23 931 931 NNP 10_1101-2021_01_08_425864 862 24 mM mM NNP 10_1101-2021_01_08_425864 862 25 histidine histidine NN 10_1101-2021_01_08_425864 862 26 , , , 10_1101-2021_01_08_425864 862 27 5 5 CD 10_1101-2021_01_08_425864 862 28 mM mm NN 10_1101-2021_01_08_425864 862 29 glucose glucose NN 10_1101-2021_01_08_425864 862 30 , , , 10_1101-2021_01_08_425864 862 31 0.1 0.1 CD 10_1101-2021_01_08_425864 862 32 mM mm NN 10_1101-2021_01_08_425864 862 33 carnitine carnitine NN 10_1101-2021_01_08_425864 862 34 and and CC 10_1101-2021_01_08_425864 862 35 BSA BSA NNP 10_1101-2021_01_08_425864 862 36 without without IN 10_1101-2021_01_08_425864 862 37 adding add VBG 10_1101-2021_01_08_425864 862 38 palmitate palmitate NN 10_1101-2021_01_08_425864 862 39 in in IN 10_1101-2021_01_08_425864 862 40 the the DT 10_1101-2021_01_08_425864 862 41 presence presence NN 10_1101-2021_01_08_425864 862 42 932 932 CD 10_1101-2021_01_08_425864 862 43 ( ( -LRB- 10_1101-2021_01_08_425864 862 44 or or CC 10_1101-2021_01_08_425864 862 45 not not RB 10_1101-2021_01_08_425864 862 46 ) ) -RRB- 10_1101-2021_01_08_425864 862 47 of of IN 10_1101-2021_01_08_425864 862 48 500 500 CD 10_1101-2021_01_08_425864 862 49 μM μM NNP 10_1101-2021_01_08_425864 862 50 ETO ETO NNP 10_1101-2021_01_08_425864 862 51 . . . 10_1101-2021_01_08_425864 863 1 The the DT 10_1101-2021_01_08_425864 863 2 viability viability NN 10_1101-2021_01_08_425864 863 3 of of IN 10_1101-2021_01_08_425864 863 4 these these DT 10_1101-2021_01_08_425864 863 5 cells cell NNS 10_1101-2021_01_08_425864 863 6 was be VBD 10_1101-2021_01_08_425864 863 7 inferred infer VBN 10_1101-2021_01_08_425864 863 8 from from IN 10_1101-2021_01_08_425864 863 9 the the DT 10_1101-2021_01_08_425864 863 10 measured measure VBN 10_1101-2021_01_08_425864 863 11 total total JJ 10_1101-2021_01_08_425864 863 12 reductive reductive JJ 10_1101-2021_01_08_425864 863 13 933 933 CD 10_1101-2021_01_08_425864 863 14 activity activity NN 10_1101-2021_01_08_425864 863 15 using use VBG 10_1101-2021_01_08_425864 863 16 MTT MTT NNP 10_1101-2021_01_08_425864 863 17 assays assays RB 10_1101-2021_01_08_425864 863 18 ( ( -LRB- 10_1101-2021_01_08_425864 863 19 see see VB 10_1101-2021_01_08_425864 863 20 Material Material NNP 10_1101-2021_01_08_425864 863 21 and and CC 10_1101-2021_01_08_425864 863 22 Methods Methods NNP 10_1101-2021_01_08_425864 863 23 section section NN 10_1101-2021_01_08_425864 863 24 for for IN 10_1101-2021_01_08_425864 863 25 more more JJR 10_1101-2021_01_08_425864 863 26 details detail NNS 10_1101-2021_01_08_425864 863 27 ) ) -RRB- 10_1101-2021_01_08_425864 863 28 . . . 10_1101-2021_01_08_425864 864 1 As as IN 10_1101-2021_01_08_425864 864 2 expected expect VBN 10_1101-2021_01_08_425864 864 3 , , , 10_1101-2021_01_08_425864 864 4 ETO eto VB 10_1101-2021_01_08_425864 864 5 934 934 CD 10_1101-2021_01_08_425864 864 6 treatment treatment NN 10_1101-2021_01_08_425864 864 7 did do VBD 10_1101-2021_01_08_425864 864 8 not not RB 10_1101-2021_01_08_425864 864 9 affect affect VB 10_1101-2021_01_08_425864 864 10 the the DT 10_1101-2021_01_08_425864 864 11 viability viability NN 10_1101-2021_01_08_425864 864 12 of of IN 10_1101-2021_01_08_425864 864 13 cells cell NNS 10_1101-2021_01_08_425864 864 14 incubated incubate VBN 10_1101-2021_01_08_425864 864 15 in in IN 10_1101-2021_01_08_425864 864 16 glucose glucose NN 10_1101-2021_01_08_425864 864 17 or or CC 10_1101-2021_01_08_425864 864 18 histidine histidine NN 10_1101-2021_01_08_425864 864 19 but but CC 10_1101-2021_01_08_425864 864 20 did do VBD 10_1101-2021_01_08_425864 864 21 affect affect VB 10_1101-2021_01_08_425864 864 22 the the DT 10_1101-2021_01_08_425864 864 23 935 935 CD 10_1101-2021_01_08_425864 864 24 viability viability NN 10_1101-2021_01_08_425864 864 25 of of IN 10_1101-2021_01_08_425864 864 26 the the DT 10_1101-2021_01_08_425864 864 27 cells cell NNS 10_1101-2021_01_08_425864 864 28 incubated incubate VBN 10_1101-2021_01_08_425864 864 29 with with IN 10_1101-2021_01_08_425864 864 30 palmitate palmitate NN 10_1101-2021_01_08_425864 864 31 or or CC 10_1101-2021_01_08_425864 864 32 carnitine carnitine NN 10_1101-2021_01_08_425864 864 33 . . . 10_1101-2021_01_08_425864 865 1 Surprisingly surprisingly RB 10_1101-2021_01_08_425864 865 2 , , , 10_1101-2021_01_08_425864 865 3 we -PRON- PRP 10_1101-2021_01_08_425864 865 4 also also RB 10_1101-2021_01_08_425864 865 5 observed observe VBD 10_1101-2021_01_08_425864 865 6 an an DT 10_1101-2021_01_08_425864 865 7 ETO eto NN 10_1101-2021_01_08_425864 865 8 936 936 CD 10_1101-2021_01_08_425864 865 9 effect effect NN 10_1101-2021_01_08_425864 865 10 on on IN 10_1101-2021_01_08_425864 865 11 parasites parasite NNS 10_1101-2021_01_08_425864 865 12 under under IN 10_1101-2021_01_08_425864 865 13 metabolic metabolic JJ 10_1101-2021_01_08_425864 865 14 stress stress NN 10_1101-2021_01_08_425864 865 15 , , , 10_1101-2021_01_08_425864 865 16 such such JJ 10_1101-2021_01_08_425864 865 17 as as IN 10_1101-2021_01_08_425864 865 18 those those DT 10_1101-2021_01_08_425864 865 19 incubated incubate VBN 10_1101-2021_01_08_425864 865 20 with with IN 10_1101-2021_01_08_425864 865 21 PBS PBS NNP 10_1101-2021_01_08_425864 865 22 or or CC 10_1101-2021_01_08_425864 865 23 BSA BSA NNP 10_1101-2021_01_08_425864 865 24 . . . 10_1101-2021_01_08_425864 866 1 This this DT 10_1101-2021_01_08_425864 866 2 finding finding NN 10_1101-2021_01_08_425864 866 3 937 937 CD 10_1101-2021_01_08_425864 866 4 could could MD 10_1101-2021_01_08_425864 866 5 be be VB 10_1101-2021_01_08_425864 866 6 explained explain VBN 10_1101-2021_01_08_425864 866 7 by by IN 10_1101-2021_01_08_425864 866 8 the the DT 10_1101-2021_01_08_425864 866 9 fact fact NN 10_1101-2021_01_08_425864 866 10 that that IN 10_1101-2021_01_08_425864 866 11 under under IN 10_1101-2021_01_08_425864 866 12 metabolic metabolic JJ 10_1101-2021_01_08_425864 866 13 stress stress NN 10_1101-2021_01_08_425864 866 14 , , , 10_1101-2021_01_08_425864 866 15 the the DT 10_1101-2021_01_08_425864 866 16 parasite parasite NN 10_1101-2021_01_08_425864 866 17 mobilizes mobilize NNS 10_1101-2021_01_08_425864 866 18 and and CC 10_1101-2021_01_08_425864 866 19 consumes consume VBZ 10_1101-2021_01_08_425864 866 20 its -PRON- PRP$ 10_1101-2021_01_08_425864 866 21 938 938 CD 10_1101-2021_01_08_425864 866 22 internal internal JJ 10_1101-2021_01_08_425864 866 23 lipids lipid NNS 10_1101-2021_01_08_425864 866 24 . . . 10_1101-2021_01_08_425864 867 1 939 939 CD 10_1101-2021_01_08_425864 867 2 940 940 CD 10_1101-2021_01_08_425864 867 3 941 941 CD 10_1101-2021_01_08_425864 867 4 S4 s4 NN 10_1101-2021_01_08_425864 867 5 Fig Fig NNP 10_1101-2021_01_08_425864 867 6 . . . 10_1101-2021_01_08_425864 868 1 ETO ETO NNP 10_1101-2021_01_08_425864 868 2 did do VBD 10_1101-2021_01_08_425864 868 3 not not RB 10_1101-2021_01_08_425864 868 4 affect affect VB 10_1101-2021_01_08_425864 868 5 the the DT 10_1101-2021_01_08_425864 868 6 viability viability NN 10_1101-2021_01_08_425864 868 7 of of IN 10_1101-2021_01_08_425864 868 8 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 868 9 forms form NNS 10_1101-2021_01_08_425864 868 10 in in IN 10_1101-2021_01_08_425864 868 11 the the DT 10_1101-2021_01_08_425864 868 12 presence presence NN 10_1101-2021_01_08_425864 868 13 of of IN 10_1101-2021_01_08_425864 868 14 other other JJ 10_1101-2021_01_08_425864 868 15 carbon carbon NN 10_1101-2021_01_08_425864 868 16 942 942 CD 10_1101-2021_01_08_425864 868 17 sources source NNS 10_1101-2021_01_08_425864 868 18 . . . 10_1101-2021_01_08_425864 869 1 The the DT 10_1101-2021_01_08_425864 869 2 viability viability NN 10_1101-2021_01_08_425864 869 3 of of IN 10_1101-2021_01_08_425864 869 4 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 869 5 forms form NNS 10_1101-2021_01_08_425864 869 6 after after IN 10_1101-2021_01_08_425864 869 7 incubation incubation NN 10_1101-2021_01_08_425864 869 8 with with IN 10_1101-2021_01_08_425864 869 9 different different JJ 10_1101-2021_01_08_425864 869 10 carbon carbon NN 10_1101-2021_01_08_425864 869 11 sources source NNS 10_1101-2021_01_08_425864 869 12 and and CC 10_1101-2021_01_08_425864 869 13 943 943 CD 10_1101-2021_01_08_425864 869 14 palmitate palmitate NN 10_1101-2021_01_08_425864 869 15 . . . 10_1101-2021_01_08_425864 870 1 The the DT 10_1101-2021_01_08_425864 870 2 viability viability NN 10_1101-2021_01_08_425864 870 3 was be VBD 10_1101-2021_01_08_425864 870 4 assessed assess VBN 10_1101-2021_01_08_425864 870 5 after after IN 10_1101-2021_01_08_425864 870 6 24 24 CD 10_1101-2021_01_08_425864 870 7 h h NN 10_1101-2021_01_08_425864 870 8 using use VBG 10_1101-2021_01_08_425864 870 9 MTT MTT NNP 10_1101-2021_01_08_425864 870 10 . . . 10_1101-2021_01_08_425864 871 1 944 944 CD 10_1101-2021_01_08_425864 871 2 945 945 CD 10_1101-2021_01_08_425864 871 3 S5 s5 NN 10_1101-2021_01_08_425864 871 4 946 946 CD 10_1101-2021_01_08_425864 871 5 947 947 CD 10_1101-2021_01_08_425864 871 6 Because because IN 10_1101-2021_01_08_425864 871 7 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 871 8 occurs occur VBZ 10_1101-2021_01_08_425864 871 9 in in IN 10_1101-2021_01_08_425864 871 10 chemically chemically RB 10_1101-2021_01_08_425864 871 11 defined define VBN 10_1101-2021_01_08_425864 871 12 conditions condition NNS 10_1101-2021_01_08_425864 871 13 , , , 10_1101-2021_01_08_425864 871 14 we -PRON- PRP 10_1101-2021_01_08_425864 871 15 performed perform VBD 10_1101-2021_01_08_425864 871 16 a a DT 10_1101-2021_01_08_425864 871 17 viability viability NN 10_1101-2021_01_08_425864 871 18 assay assay NN 10_1101-2021_01_08_425864 871 19 948 948 CD 10_1101-2021_01_08_425864 871 20 to to TO 10_1101-2021_01_08_425864 871 21 define define VB 10_1101-2021_01_08_425864 871 22 the the DT 10_1101-2021_01_08_425864 871 23 maximum maximum NN 10_1101-2021_01_08_425864 871 24 tolerated tolerated JJ 10_1101-2021_01_08_425864 871 25 concentration concentration NN 10_1101-2021_01_08_425864 871 26 that that WDT 10_1101-2021_01_08_425864 871 27 allows allow VBZ 10_1101-2021_01_08_425864 871 28 the the DT 10_1101-2021_01_08_425864 871 29 parasites parasite NNS 10_1101-2021_01_08_425864 871 30 to to TO 10_1101-2021_01_08_425864 871 31 survive survive VB 10_1101-2021_01_08_425864 871 32 under under IN 10_1101-2021_01_08_425864 871 33 ETO ETO NNP 10_1101-2021_01_08_425864 871 34 949 949 CD 10_1101-2021_01_08_425864 871 35 treatment treatment NN 10_1101-2021_01_08_425864 871 36 . . . 10_1101-2021_01_08_425864 872 1 Stationary stationary JJ 10_1101-2021_01_08_425864 872 2 epimastigotes epimastigote NNS 10_1101-2021_01_08_425864 872 3 in in IN 10_1101-2021_01_08_425864 872 4 TAU-3AAG TAU-3AAG NNP 10_1101-2021_01_08_425864 872 5 media medium NNS 10_1101-2021_01_08_425864 872 6 were be VBD 10_1101-2021_01_08_425864 872 7 treated treat VBN 10_1101-2021_01_08_425864 872 8 with with IN 10_1101-2021_01_08_425864 872 9 different different JJ 10_1101-2021_01_08_425864 872 10 concentrations concentration NNS 10_1101-2021_01_08_425864 872 11 950 950 CD 10_1101-2021_01_08_425864 872 12 of of IN 10_1101-2021_01_08_425864 872 13 ETO ETO NNP 10_1101-2021_01_08_425864 872 14 ( ( -LRB- 10_1101-2021_01_08_425864 872 15 range range NNP 10_1101-2021_01_08_425864 872 16 5 5 CD 10_1101-2021_01_08_425864 872 17 to to IN 10_1101-2021_01_08_425864 872 18 500 500 CD 10_1101-2021_01_08_425864 872 19 μM μM NNS 10_1101-2021_01_08_425864 872 20 ) ) -RRB- 10_1101-2021_01_08_425864 872 21 during during IN 10_1101-2021_01_08_425864 872 22 24 24 CD 10_1101-2021_01_08_425864 872 23 h. h. NNS 10_1101-2021_01_08_425864 872 24 The the DT 10_1101-2021_01_08_425864 872 25 viability viability NN 10_1101-2021_01_08_425864 872 26 of of IN 10_1101-2021_01_08_425864 872 27 these these DT 10_1101-2021_01_08_425864 872 28 cells cell NNS 10_1101-2021_01_08_425864 872 29 was be VBD 10_1101-2021_01_08_425864 872 30 inferred infer VBN 10_1101-2021_01_08_425864 872 31 by by IN 10_1101-2021_01_08_425864 872 32 measuring measure VBG 10_1101-2021_01_08_425864 872 33 the the DT 10_1101-2021_01_08_425864 872 34 951 951 CD 10_1101-2021_01_08_425864 872 35 total total JJ 10_1101-2021_01_08_425864 872 36 reductive reductive JJ 10_1101-2021_01_08_425864 872 37 activity activity NN 10_1101-2021_01_08_425864 872 38 using use VBG 10_1101-2021_01_08_425864 872 39 an an DT 10_1101-2021_01_08_425864 872 40 Alamar Alamar NNP 10_1101-2021_01_08_425864 872 41 blue blue JJ 10_1101-2021_01_08_425864 872 42 assay assay NN 10_1101-2021_01_08_425864 872 43 [ [ -LRB- 10_1101-2021_01_08_425864 872 44 63 63 CD 10_1101-2021_01_08_425864 872 45 ] ] -RRB- 10_1101-2021_01_08_425864 872 46 . . . 10_1101-2021_01_08_425864 873 1 Briefly briefly RB 10_1101-2021_01_08_425864 873 2 , , , 10_1101-2021_01_08_425864 873 3 after after IN 10_1101-2021_01_08_425864 873 4 24 24 CD 10_1101-2021_01_08_425864 873 5 h h NN 10_1101-2021_01_08_425864 873 6 in in IN 10_1101-2021_01_08_425864 873 7 the the DT 10_1101-2021_01_08_425864 873 8 presence presence NN 10_1101-2021_01_08_425864 873 9 or or CC 10_1101-2021_01_08_425864 873 10 absence absence NN 10_1101-2021_01_08_425864 873 11 952 952 CD 10_1101-2021_01_08_425864 873 12 of of IN 10_1101-2021_01_08_425864 873 13 ETO ETO NNP 10_1101-2021_01_08_425864 873 14 , , , 10_1101-2021_01_08_425864 873 15 the the DT 10_1101-2021_01_08_425864 873 16 cells cell NNS 10_1101-2021_01_08_425864 873 17 were be VBD 10_1101-2021_01_08_425864 873 18 incubated incubate VBN 10_1101-2021_01_08_425864 873 19 with with IN 10_1101-2021_01_08_425864 873 20 0,125 0,125 CD 10_1101-2021_01_08_425864 873 21 μg.mL-1 μg.mL-1 NNS 10_1101-2021_01_08_425864 873 22 of of IN 10_1101-2021_01_08_425864 873 23 Alamar Alamar NNP 10_1101-2021_01_08_425864 873 24 blue blue JJ 10_1101-2021_01_08_425864 873 25 reagent reagent NN 10_1101-2021_01_08_425864 873 26 in in IN 10_1101-2021_01_08_425864 873 27 accordance accordance NN 10_1101-2021_01_08_425864 873 28 with with IN 10_1101-2021_01_08_425864 873 29 the the DT 10_1101-2021_01_08_425864 873 30 953 953 CD 10_1101-2021_01_08_425864 873 31 protocol protocol NN 10_1101-2021_01_08_425864 873 32 by by IN 10_1101-2021_01_08_425864 873 33 [ [ -LRB- 10_1101-2021_01_08_425864 873 34 17 17 CD 10_1101-2021_01_08_425864 873 35 ] ] -RRB- 10_1101-2021_01_08_425864 873 36 . . . 10_1101-2021_01_08_425864 874 1 Under under IN 10_1101-2021_01_08_425864 874 2 these these DT 10_1101-2021_01_08_425864 874 3 conditions condition NNS 10_1101-2021_01_08_425864 874 4 , , , 10_1101-2021_01_08_425864 874 5 the the DT 10_1101-2021_01_08_425864 874 6 parasites parasite NNS 10_1101-2021_01_08_425864 874 7 were be VBD 10_1101-2021_01_08_425864 874 8 10 10 CD 10_1101-2021_01_08_425864 874 9 times time NNS 10_1101-2021_01_08_425864 874 10 more more RBR 10_1101-2021_01_08_425864 874 11 sensitive sensitive JJ 10_1101-2021_01_08_425864 874 12 to to IN 10_1101-2021_01_08_425864 874 13 ETO ETO NNP 10_1101-2021_01_08_425864 874 14 954 954 CD 10_1101-2021_01_08_425864 874 15 treatment treatment NN 10_1101-2021_01_08_425864 874 16 , , , 10_1101-2021_01_08_425864 874 17 surviving survive VBG 10_1101-2021_01_08_425864 874 18 when when WRB 10_1101-2021_01_08_425864 874 19 subjected subject VBN 10_1101-2021_01_08_425864 874 20 to to IN 10_1101-2021_01_08_425864 874 21 ETO eto NN 10_1101-2021_01_08_425864 874 22 concentrations concentration NNS 10_1101-2021_01_08_425864 874 23 between between IN 10_1101-2021_01_08_425864 874 24 5 5 CD 10_1101-2021_01_08_425864 874 25 - - SYM 10_1101-2021_01_08_425864 874 26 50 50 CD 10_1101-2021_01_08_425864 874 27 µM µM NNP 10_1101-2021_01_08_425864 874 28 ( ( -LRB- 10_1101-2021_01_08_425864 874 29 Fig Fig NNP 10_1101-2021_01_08_425864 874 30 . . . 10_1101-2021_01_08_425864 875 1 S3 S3 NNP 10_1101-2021_01_08_425864 875 2 A A NNP 10_1101-2021_01_08_425864 875 3 ) ) -RRB- 10_1101-2021_01_08_425864 875 4 . . . 10_1101-2021_01_08_425864 876 1 This this DT 10_1101-2021_01_08_425864 876 2 range range NN 10_1101-2021_01_08_425864 876 3 955 955 CD 10_1101-2021_01_08_425864 876 4 of of IN 10_1101-2021_01_08_425864 876 5 concentrations concentration NNS 10_1101-2021_01_08_425864 876 6 used use VBN 10_1101-2021_01_08_425864 876 7 to to TO 10_1101-2021_01_08_425864 876 8 treat treat VB 10_1101-2021_01_08_425864 876 9 the the DT 10_1101-2021_01_08_425864 876 10 parasites parasite NNS 10_1101-2021_01_08_425864 876 11 was be VBD 10_1101-2021_01_08_425864 876 12 maintained maintain VBN 10_1101-2021_01_08_425864 876 13 in in IN 10_1101-2021_01_08_425864 876 14 TAU-3AAG TAU-3AAG NNP 10_1101-2021_01_08_425864 876 15 medium medium NNP 10_1101-2021_01_08_425864 876 16 and and CC 10_1101-2021_01_08_425864 876 17 to to TO 10_1101-2021_01_08_425864 876 18 follow follow VB 10_1101-2021_01_08_425864 876 19 956 956 CD 10_1101-2021_01_08_425864 876 20 the the DT 10_1101-2021_01_08_425864 876 21 differentiation differentiation NN 10_1101-2021_01_08_425864 876 22 by by IN 10_1101-2021_01_08_425864 876 23 daily daily JJ 10_1101-2021_01_08_425864 876 24 counts count NNS 10_1101-2021_01_08_425864 876 25 , , , 10_1101-2021_01_08_425864 876 26 based base VBN 10_1101-2021_01_08_425864 876 27 on on IN 10_1101-2021_01_08_425864 876 28 the the DT 10_1101-2021_01_08_425864 876 29 percentage percentage NN 10_1101-2021_01_08_425864 876 30 of of IN 10_1101-2021_01_08_425864 876 31 metacyclic metacyclic JJ 10_1101-2021_01_08_425864 876 32 trypomastigotes trypomastigote NNS 10_1101-2021_01_08_425864 876 33 collected collect VBD 10_1101-2021_01_08_425864 876 34 957 957 CD 10_1101-2021_01_08_425864 876 35 in in IN 10_1101-2021_01_08_425864 876 36 culture culture NN 10_1101-2021_01_08_425864 876 37 supernatant supernatant JJ 10_1101-2021_01_08_425864 876 38 . . . 10_1101-2021_01_08_425864 877 1 To to TO 10_1101-2021_01_08_425864 877 2 confirm confirm VB 10_1101-2021_01_08_425864 877 3 that that IN 10_1101-2021_01_08_425864 877 4 the the DT 10_1101-2021_01_08_425864 877 5 parasites parasite NNS 10_1101-2021_01_08_425864 877 6 were be VBD 10_1101-2021_01_08_425864 877 7 still still RB 10_1101-2021_01_08_425864 877 8 alive alive JJ 10_1101-2021_01_08_425864 877 9 after after IN 10_1101-2021_01_08_425864 877 10 5 5 CD 10_1101-2021_01_08_425864 877 11 days day NNS 10_1101-2021_01_08_425864 877 12 under under IN 10_1101-2021_01_08_425864 877 13 differentiation differentiation NN 10_1101-2021_01_08_425864 877 14 , , , 10_1101-2021_01_08_425864 877 15 958 958 CD 10_1101-2021_01_08_425864 877 16 we -PRON- PRP 10_1101-2021_01_08_425864 877 17 checked check VBD 10_1101-2021_01_08_425864 877 18 the the DT 10_1101-2021_01_08_425864 877 19 viability viability NN 10_1101-2021_01_08_425864 877 20 of of IN 10_1101-2021_01_08_425864 877 21 cells cell NNS 10_1101-2021_01_08_425864 877 22 that that WDT 10_1101-2021_01_08_425864 877 23 were be VBD 10_1101-2021_01_08_425864 877 24 treated treat VBN 10_1101-2021_01_08_425864 877 25 ( ( -LRB- 10_1101-2021_01_08_425864 877 26 or or CC 10_1101-2021_01_08_425864 877 27 not not RB 10_1101-2021_01_08_425864 877 28 , , , 10_1101-2021_01_08_425864 877 29 control control NN 10_1101-2021_01_08_425864 877 30 ) ) -RRB- 10_1101-2021_01_08_425864 877 31 using use VBG 10_1101-2021_01_08_425864 877 32 the the DT 10_1101-2021_01_08_425864 877 33 same same JJ 10_1101-2021_01_08_425864 877 34 assay assay NN 10_1101-2021_01_08_425864 877 35 . . . 10_1101-2021_01_08_425864 878 1 As as IN 10_1101-2021_01_08_425864 878 2 shown show VBN 10_1101-2021_01_08_425864 878 3 959 959 CD 10_1101-2021_01_08_425864 878 4 above above IN 10_1101-2021_01_08_425864 878 5 ( ( -LRB- 10_1101-2021_01_08_425864 878 6 Figure figure NN 10_1101-2021_01_08_425864 878 7 S3 S3 NNP 10_1101-2021_01_08_425864 878 8 B B NNP 10_1101-2021_01_08_425864 878 9 ) ) -RRB- 10_1101-2021_01_08_425864 878 10 , , , 10_1101-2021_01_08_425864 878 11 the the DT 10_1101-2021_01_08_425864 878 12 parasites parasite NNS 10_1101-2021_01_08_425864 878 13 were be VBD 10_1101-2021_01_08_425864 878 14 viable viable JJ 10_1101-2021_01_08_425864 878 15 under under IN 10_1101-2021_01_08_425864 878 16 all all PDT 10_1101-2021_01_08_425864 878 17 the the DT 10_1101-2021_01_08_425864 878 18 tested test VBN 10_1101-2021_01_08_425864 878 19 conditions condition NNS 10_1101-2021_01_08_425864 878 20 . . . 10_1101-2021_01_08_425864 879 1 Considering consider VBG 10_1101-2021_01_08_425864 879 2 that that IN 10_1101-2021_01_08_425864 879 3 TAU- tau- NN 10_1101-2021_01_08_425864 879 4 960 960 CD 10_1101-2021_01_08_425864 879 5 3AAG 3aag NN 10_1101-2021_01_08_425864 879 6 contains contain VBZ 10_1101-2021_01_08_425864 879 7 glucose glucose NN 10_1101-2021_01_08_425864 879 8 in in IN 10_1101-2021_01_08_425864 879 9 its -PRON- PRP$ 10_1101-2021_01_08_425864 879 10 composition composition NN 10_1101-2021_01_08_425864 879 11 , , , 10_1101-2021_01_08_425864 879 12 we -PRON- PRP 10_1101-2021_01_08_425864 879 13 performed perform VBD 10_1101-2021_01_08_425864 879 14 an an DT 10_1101-2021_01_08_425864 879 15 in in FW 10_1101-2021_01_08_425864 879 16 vitro vitro FW 10_1101-2021_01_08_425864 879 17 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 879 18 using use VBG 10_1101-2021_01_08_425864 879 19 only only RB 10_1101-2021_01_08_425864 879 20 961 961 CD 10_1101-2021_01_08_425864 879 21 proline proline NN 10_1101-2021_01_08_425864 879 22 as as IN 10_1101-2021_01_08_425864 879 23 a a DT 10_1101-2021_01_08_425864 879 24 metabolic metabolic JJ 10_1101-2021_01_08_425864 879 25 inducer inducer NN 10_1101-2021_01_08_425864 879 26 [ [ -LRB- 10_1101-2021_01_08_425864 879 27 64 64 CD 10_1101-2021_01_08_425864 879 28 ] ] -RRB- 10_1101-2021_01_08_425864 879 29 . . . 10_1101-2021_01_08_425864 880 1 As as IN 10_1101-2021_01_08_425864 880 2 observed observe VBN 10_1101-2021_01_08_425864 880 3 , , , 10_1101-2021_01_08_425864 880 4 even even RB 10_1101-2021_01_08_425864 880 5 in in IN 10_1101-2021_01_08_425864 880 6 the the DT 10_1101-2021_01_08_425864 880 7 absence absence NN 10_1101-2021_01_08_425864 880 8 of of IN 10_1101-2021_01_08_425864 880 9 glucose glucose NN 10_1101-2021_01_08_425864 880 10 , , , 10_1101-2021_01_08_425864 880 11 ETO ETO NNP 10_1101-2021_01_08_425864 880 12 treatment treatment NN 10_1101-2021_01_08_425864 880 13 962 962 CD 10_1101-2021_01_08_425864 880 14 affects affect NNS 10_1101-2021_01_08_425864 880 15 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 880 16 . . . 10_1101-2021_01_08_425864 881 1 963 963 CD 10_1101-2021_01_08_425864 881 2 964 964 CD 10_1101-2021_01_08_425864 881 3 965 965 CD 10_1101-2021_01_08_425864 881 4 966 966 CD 10_1101-2021_01_08_425864 881 5 Fig Fig NNP 10_1101-2021_01_08_425864 881 6 S5 s5 NN 10_1101-2021_01_08_425864 881 7 . . . 10_1101-2021_01_08_425864 882 1 Viability viability NN 10_1101-2021_01_08_425864 882 2 of of IN 10_1101-2021_01_08_425864 882 3 epimastigote epimastigote JJ 10_1101-2021_01_08_425864 882 4 forms form NNS 10_1101-2021_01_08_425864 882 5 subjected subject VBN 10_1101-2021_01_08_425864 882 6 to to IN 10_1101-2021_01_08_425864 882 7 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 882 8 under under IN 10_1101-2021_01_08_425864 882 9 different different JJ 10_1101-2021_01_08_425864 882 10 ETO ETO NNP 10_1101-2021_01_08_425864 882 11 967 967 CD 10_1101-2021_01_08_425864 882 12 concentrations concentration NNS 10_1101-2021_01_08_425864 882 13 . . . 10_1101-2021_01_08_425864 883 1 A a LS 10_1101-2021_01_08_425864 883 2 ) ) -RRB- 10_1101-2021_01_08_425864 883 3 Cell cell NN 10_1101-2021_01_08_425864 883 4 viability viability NN 10_1101-2021_01_08_425864 883 5 under under IN 10_1101-2021_01_08_425864 883 6 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 883 7 after after IN 10_1101-2021_01_08_425864 883 8 24 24 CD 10_1101-2021_01_08_425864 883 9 h h NN 10_1101-2021_01_08_425864 883 10 of of IN 10_1101-2021_01_08_425864 883 11 treatment treatment NN 10_1101-2021_01_08_425864 883 12 with with IN 10_1101-2021_01_08_425864 883 13 different different JJ 10_1101-2021_01_08_425864 883 14 ETO ETO NNP 10_1101-2021_01_08_425864 883 15 968 968 CD 10_1101-2021_01_08_425864 883 16 concentrations concentration NNS 10_1101-2021_01_08_425864 883 17 . . . 10_1101-2021_01_08_425864 884 1 B b NN 10_1101-2021_01_08_425864 884 2 ) ) -RRB- 10_1101-2021_01_08_425864 884 3 Cell cell NN 10_1101-2021_01_08_425864 884 4 viability viability NN 10_1101-2021_01_08_425864 884 5 under under IN 10_1101-2021_01_08_425864 884 6 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 884 7 after after IN 10_1101-2021_01_08_425864 884 8 5 5 CD 10_1101-2021_01_08_425864 884 9 days day NNS 10_1101-2021_01_08_425864 884 10 in in IN 10_1101-2021_01_08_425864 884 11 the the DT 10_1101-2021_01_08_425864 884 12 presence presence NN 10_1101-2021_01_08_425864 884 13 of of IN 10_1101-2021_01_08_425864 884 14 ETO ETO NNP 10_1101-2021_01_08_425864 884 15 . . . 10_1101-2021_01_08_425864 885 1 C C NNP 10_1101-2021_01_08_425864 885 2 ) ) -RRB- 10_1101-2021_01_08_425864 885 3 969 969 CD 10_1101-2021_01_08_425864 885 4 Effect effect NN 10_1101-2021_01_08_425864 885 5 of of IN 10_1101-2021_01_08_425864 885 6 ETO ETO NNP 10_1101-2021_01_08_425864 885 7 on on IN 10_1101-2021_01_08_425864 885 8 the the DT 10_1101-2021_01_08_425864 885 9 metacyclogenesis metacyclogenesis NN 10_1101-2021_01_08_425864 885 10 induced induce VBN 10_1101-2021_01_08_425864 885 11 by by IN 10_1101-2021_01_08_425864 885 12 proline proline NN 10_1101-2021_01_08_425864 885 13 . . . 10_1101-2021_01_08_425864 886 1 970 970 CD 10_1101-2021_01_08_425864 886 2 .CC .CC : 10_1101-2021_01_08_425864 886 3 - - : 10_1101-2021_01_08_425864 886 4 BY by IN 10_1101-2021_01_08_425864 886 5 4.0 4.0 CD 10_1101-2021_01_08_425864 886 6 International international JJ 10_1101-2021_01_08_425864 886 7 licensemade licensemade NN 10_1101-2021_01_08_425864 886 8 available available JJ 10_1101-2021_01_08_425864 886 9 under under IN 10_1101-2021_01_08_425864 886 10 a a DT 10_1101-2021_01_08_425864 886 11 ( ( -LRB- 10_1101-2021_01_08_425864 886 12 which which WDT 10_1101-2021_01_08_425864 886 13 was be VBD 10_1101-2021_01_08_425864 886 14 not not RB 10_1101-2021_01_08_425864 886 15 certified certify VBN 10_1101-2021_01_08_425864 886 16 by by IN 10_1101-2021_01_08_425864 886 17 peer peer NN 10_1101-2021_01_08_425864 886 18 review review NN 10_1101-2021_01_08_425864 886 19 ) ) -RRB- 10_1101-2021_01_08_425864 886 20 is be VBZ 10_1101-2021_01_08_425864 886 21 the the DT 10_1101-2021_01_08_425864 886 22 author author NN 10_1101-2021_01_08_425864 886 23 / / SYM 10_1101-2021_01_08_425864 886 24 funder funder NN 10_1101-2021_01_08_425864 886 25 , , , 10_1101-2021_01_08_425864 886 26 who who WP 10_1101-2021_01_08_425864 886 27 has have VBZ 10_1101-2021_01_08_425864 886 28 granted grant VBN 10_1101-2021_01_08_425864 886 29 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 886 30 a a DT 10_1101-2021_01_08_425864 886 31 license license NN 10_1101-2021_01_08_425864 886 32 to to TO 10_1101-2021_01_08_425864 886 33 display display VB 10_1101-2021_01_08_425864 886 34 the the DT 10_1101-2021_01_08_425864 886 35 preprint preprint NN 10_1101-2021_01_08_425864 886 36 in in IN 10_1101-2021_01_08_425864 886 37 perpetuity perpetuity NN 10_1101-2021_01_08_425864 886 38 . . . 10_1101-2021_01_08_425864 887 1 It -PRON- PRP 10_1101-2021_01_08_425864 887 2 is be VBZ 10_1101-2021_01_08_425864 887 3 The the DT 10_1101-2021_01_08_425864 887 4 copyright copyright NN 10_1101-2021_01_08_425864 887 5 holder holder NN 10_1101-2021_01_08_425864 887 6 for for IN 10_1101-2021_01_08_425864 887 7 this this DT 10_1101-2021_01_08_425864 887 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 887 9 version version NN 10_1101-2021_01_08_425864 887 10 posted post VBD 10_1101-2021_01_08_425864 887 11 January January NNP 10_1101-2021_01_08_425864 887 12 8 8 CD 10_1101-2021_01_08_425864 887 13 , , , 10_1101-2021_01_08_425864 887 14 2021 2021 CD 10_1101-2021_01_08_425864 887 15 . . . 10_1101-2021_01_08_425864 887 16 ; ; : 10_1101-2021_01_08_425864 887 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 887 18 : : : 10_1101-2021_01_08_425864 887 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 887 20 preprint preprint JJ 10_1101-2021_01_08_425864 887 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 887 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 887 23 .CC .CC NFP 10_1101-2021_01_08_425864 887 24 - - : 10_1101-2021_01_08_425864 887 25 BY by IN 10_1101-2021_01_08_425864 887 26 4.0 4.0 CD 10_1101-2021_01_08_425864 887 27 International international JJ 10_1101-2021_01_08_425864 887 28 licensemade licensemade NN 10_1101-2021_01_08_425864 887 29 available available JJ 10_1101-2021_01_08_425864 887 30 under under IN 10_1101-2021_01_08_425864 887 31 a a DT 10_1101-2021_01_08_425864 887 32 ( ( -LRB- 10_1101-2021_01_08_425864 887 33 which which WDT 10_1101-2021_01_08_425864 887 34 was be VBD 10_1101-2021_01_08_425864 887 35 not not RB 10_1101-2021_01_08_425864 887 36 certified certify VBN 10_1101-2021_01_08_425864 887 37 by by IN 10_1101-2021_01_08_425864 887 38 peer peer NN 10_1101-2021_01_08_425864 887 39 review review NN 10_1101-2021_01_08_425864 887 40 ) ) -RRB- 10_1101-2021_01_08_425864 887 41 is be VBZ 10_1101-2021_01_08_425864 887 42 the the DT 10_1101-2021_01_08_425864 887 43 author author NN 10_1101-2021_01_08_425864 887 44 / / SYM 10_1101-2021_01_08_425864 887 45 funder funder NN 10_1101-2021_01_08_425864 887 46 , , , 10_1101-2021_01_08_425864 887 47 who who WP 10_1101-2021_01_08_425864 887 48 has have VBZ 10_1101-2021_01_08_425864 887 49 granted grant VBN 10_1101-2021_01_08_425864 887 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 887 51 a a DT 10_1101-2021_01_08_425864 887 52 license license NN 10_1101-2021_01_08_425864 887 53 to to TO 10_1101-2021_01_08_425864 887 54 display display VB 10_1101-2021_01_08_425864 887 55 the the DT 10_1101-2021_01_08_425864 887 56 preprint preprint NN 10_1101-2021_01_08_425864 887 57 in in IN 10_1101-2021_01_08_425864 887 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 887 59 . . . 10_1101-2021_01_08_425864 888 1 It -PRON- PRP 10_1101-2021_01_08_425864 888 2 is be VBZ 10_1101-2021_01_08_425864 888 3 The the DT 10_1101-2021_01_08_425864 888 4 copyright copyright NN 10_1101-2021_01_08_425864 888 5 holder holder NN 10_1101-2021_01_08_425864 888 6 for for IN 10_1101-2021_01_08_425864 888 7 this this DT 10_1101-2021_01_08_425864 888 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 888 9 version version NN 10_1101-2021_01_08_425864 888 10 posted post VBD 10_1101-2021_01_08_425864 888 11 January January NNP 10_1101-2021_01_08_425864 888 12 8 8 CD 10_1101-2021_01_08_425864 888 13 , , , 10_1101-2021_01_08_425864 888 14 2021 2021 CD 10_1101-2021_01_08_425864 888 15 . . . 10_1101-2021_01_08_425864 888 16 ; ; : 10_1101-2021_01_08_425864 888 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 888 18 : : : 10_1101-2021_01_08_425864 888 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 888 20 preprint preprint JJ 10_1101-2021_01_08_425864 888 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 888 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 888 23 .CC .CC NFP 10_1101-2021_01_08_425864 888 24 - - : 10_1101-2021_01_08_425864 888 25 BY by IN 10_1101-2021_01_08_425864 888 26 4.0 4.0 CD 10_1101-2021_01_08_425864 888 27 International international JJ 10_1101-2021_01_08_425864 888 28 licensemade licensemade NN 10_1101-2021_01_08_425864 888 29 available available JJ 10_1101-2021_01_08_425864 888 30 under under IN 10_1101-2021_01_08_425864 888 31 a a DT 10_1101-2021_01_08_425864 888 32 ( ( -LRB- 10_1101-2021_01_08_425864 888 33 which which WDT 10_1101-2021_01_08_425864 888 34 was be VBD 10_1101-2021_01_08_425864 888 35 not not RB 10_1101-2021_01_08_425864 888 36 certified certify VBN 10_1101-2021_01_08_425864 888 37 by by IN 10_1101-2021_01_08_425864 888 38 peer peer NN 10_1101-2021_01_08_425864 888 39 review review NN 10_1101-2021_01_08_425864 888 40 ) ) -RRB- 10_1101-2021_01_08_425864 888 41 is be VBZ 10_1101-2021_01_08_425864 888 42 the the DT 10_1101-2021_01_08_425864 888 43 author author NN 10_1101-2021_01_08_425864 888 44 / / SYM 10_1101-2021_01_08_425864 888 45 funder funder NN 10_1101-2021_01_08_425864 888 46 , , , 10_1101-2021_01_08_425864 888 47 who who WP 10_1101-2021_01_08_425864 888 48 has have VBZ 10_1101-2021_01_08_425864 888 49 granted grant VBN 10_1101-2021_01_08_425864 888 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 888 51 a a DT 10_1101-2021_01_08_425864 888 52 license license NN 10_1101-2021_01_08_425864 888 53 to to TO 10_1101-2021_01_08_425864 888 54 display display VB 10_1101-2021_01_08_425864 888 55 the the DT 10_1101-2021_01_08_425864 888 56 preprint preprint NN 10_1101-2021_01_08_425864 888 57 in in IN 10_1101-2021_01_08_425864 888 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 888 59 . . . 10_1101-2021_01_08_425864 889 1 It -PRON- PRP 10_1101-2021_01_08_425864 889 2 is be VBZ 10_1101-2021_01_08_425864 889 3 The the DT 10_1101-2021_01_08_425864 889 4 copyright copyright NN 10_1101-2021_01_08_425864 889 5 holder holder NN 10_1101-2021_01_08_425864 889 6 for for IN 10_1101-2021_01_08_425864 889 7 this this DT 10_1101-2021_01_08_425864 889 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 889 9 version version NN 10_1101-2021_01_08_425864 889 10 posted post VBD 10_1101-2021_01_08_425864 889 11 January January NNP 10_1101-2021_01_08_425864 889 12 8 8 CD 10_1101-2021_01_08_425864 889 13 , , , 10_1101-2021_01_08_425864 889 14 2021 2021 CD 10_1101-2021_01_08_425864 889 15 . . . 10_1101-2021_01_08_425864 889 16 ; ; : 10_1101-2021_01_08_425864 889 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 889 18 : : : 10_1101-2021_01_08_425864 889 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 889 20 preprint preprint JJ 10_1101-2021_01_08_425864 889 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 889 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 889 23 .CC .CC NFP 10_1101-2021_01_08_425864 889 24 - - : 10_1101-2021_01_08_425864 889 25 BY by IN 10_1101-2021_01_08_425864 889 26 4.0 4.0 CD 10_1101-2021_01_08_425864 889 27 International international JJ 10_1101-2021_01_08_425864 889 28 licensemade licensemade NN 10_1101-2021_01_08_425864 889 29 available available JJ 10_1101-2021_01_08_425864 889 30 under under IN 10_1101-2021_01_08_425864 889 31 a a DT 10_1101-2021_01_08_425864 889 32 ( ( -LRB- 10_1101-2021_01_08_425864 889 33 which which WDT 10_1101-2021_01_08_425864 889 34 was be VBD 10_1101-2021_01_08_425864 889 35 not not RB 10_1101-2021_01_08_425864 889 36 certified certify VBN 10_1101-2021_01_08_425864 889 37 by by IN 10_1101-2021_01_08_425864 889 38 peer peer NN 10_1101-2021_01_08_425864 889 39 review review NN 10_1101-2021_01_08_425864 889 40 ) ) -RRB- 10_1101-2021_01_08_425864 889 41 is be VBZ 10_1101-2021_01_08_425864 889 42 the the DT 10_1101-2021_01_08_425864 889 43 author author NN 10_1101-2021_01_08_425864 889 44 / / SYM 10_1101-2021_01_08_425864 889 45 funder funder NN 10_1101-2021_01_08_425864 889 46 , , , 10_1101-2021_01_08_425864 889 47 who who WP 10_1101-2021_01_08_425864 889 48 has have VBZ 10_1101-2021_01_08_425864 889 49 granted grant VBN 10_1101-2021_01_08_425864 889 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 889 51 a a DT 10_1101-2021_01_08_425864 889 52 license license NN 10_1101-2021_01_08_425864 889 53 to to TO 10_1101-2021_01_08_425864 889 54 display display VB 10_1101-2021_01_08_425864 889 55 the the DT 10_1101-2021_01_08_425864 889 56 preprint preprint NN 10_1101-2021_01_08_425864 889 57 in in IN 10_1101-2021_01_08_425864 889 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 889 59 . . . 10_1101-2021_01_08_425864 890 1 It -PRON- PRP 10_1101-2021_01_08_425864 890 2 is be VBZ 10_1101-2021_01_08_425864 890 3 The the DT 10_1101-2021_01_08_425864 890 4 copyright copyright NN 10_1101-2021_01_08_425864 890 5 holder holder NN 10_1101-2021_01_08_425864 890 6 for for IN 10_1101-2021_01_08_425864 890 7 this this DT 10_1101-2021_01_08_425864 890 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 890 9 version version NN 10_1101-2021_01_08_425864 890 10 posted post VBD 10_1101-2021_01_08_425864 890 11 January January NNP 10_1101-2021_01_08_425864 890 12 8 8 CD 10_1101-2021_01_08_425864 890 13 , , , 10_1101-2021_01_08_425864 890 14 2021 2021 CD 10_1101-2021_01_08_425864 890 15 . . . 10_1101-2021_01_08_425864 890 16 ; ; : 10_1101-2021_01_08_425864 890 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 890 18 : : : 10_1101-2021_01_08_425864 890 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 890 20 preprint preprint JJ 10_1101-2021_01_08_425864 890 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 890 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 890 23 .CC .CC NFP 10_1101-2021_01_08_425864 890 24 - - : 10_1101-2021_01_08_425864 890 25 BY by IN 10_1101-2021_01_08_425864 890 26 4.0 4.0 CD 10_1101-2021_01_08_425864 890 27 International international JJ 10_1101-2021_01_08_425864 890 28 licensemade licensemade NN 10_1101-2021_01_08_425864 890 29 available available JJ 10_1101-2021_01_08_425864 890 30 under under IN 10_1101-2021_01_08_425864 890 31 a a DT 10_1101-2021_01_08_425864 890 32 ( ( -LRB- 10_1101-2021_01_08_425864 890 33 which which WDT 10_1101-2021_01_08_425864 890 34 was be VBD 10_1101-2021_01_08_425864 890 35 not not RB 10_1101-2021_01_08_425864 890 36 certified certify VBN 10_1101-2021_01_08_425864 890 37 by by IN 10_1101-2021_01_08_425864 890 38 peer peer NN 10_1101-2021_01_08_425864 890 39 review review NN 10_1101-2021_01_08_425864 890 40 ) ) -RRB- 10_1101-2021_01_08_425864 890 41 is be VBZ 10_1101-2021_01_08_425864 890 42 the the DT 10_1101-2021_01_08_425864 890 43 author author NN 10_1101-2021_01_08_425864 890 44 / / SYM 10_1101-2021_01_08_425864 890 45 funder funder NN 10_1101-2021_01_08_425864 890 46 , , , 10_1101-2021_01_08_425864 890 47 who who WP 10_1101-2021_01_08_425864 890 48 has have VBZ 10_1101-2021_01_08_425864 890 49 granted grant VBN 10_1101-2021_01_08_425864 890 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 890 51 a a DT 10_1101-2021_01_08_425864 890 52 license license NN 10_1101-2021_01_08_425864 890 53 to to TO 10_1101-2021_01_08_425864 890 54 display display VB 10_1101-2021_01_08_425864 890 55 the the DT 10_1101-2021_01_08_425864 890 56 preprint preprint NN 10_1101-2021_01_08_425864 890 57 in in IN 10_1101-2021_01_08_425864 890 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 890 59 . . . 10_1101-2021_01_08_425864 891 1 It -PRON- PRP 10_1101-2021_01_08_425864 891 2 is be VBZ 10_1101-2021_01_08_425864 891 3 The the DT 10_1101-2021_01_08_425864 891 4 copyright copyright NN 10_1101-2021_01_08_425864 891 5 holder holder NN 10_1101-2021_01_08_425864 891 6 for for IN 10_1101-2021_01_08_425864 891 7 this this DT 10_1101-2021_01_08_425864 891 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 891 9 version version NN 10_1101-2021_01_08_425864 891 10 posted post VBD 10_1101-2021_01_08_425864 891 11 January January NNP 10_1101-2021_01_08_425864 891 12 8 8 CD 10_1101-2021_01_08_425864 891 13 , , , 10_1101-2021_01_08_425864 891 14 2021 2021 CD 10_1101-2021_01_08_425864 891 15 . . . 10_1101-2021_01_08_425864 891 16 ; ; : 10_1101-2021_01_08_425864 891 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 891 18 : : : 10_1101-2021_01_08_425864 891 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 891 20 preprint preprint JJ 10_1101-2021_01_08_425864 891 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 891 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 891 23 .CC .CC NFP 10_1101-2021_01_08_425864 891 24 - - : 10_1101-2021_01_08_425864 891 25 BY by IN 10_1101-2021_01_08_425864 891 26 4.0 4.0 CD 10_1101-2021_01_08_425864 891 27 International international JJ 10_1101-2021_01_08_425864 891 28 licensemade licensemade NN 10_1101-2021_01_08_425864 891 29 available available JJ 10_1101-2021_01_08_425864 891 30 under under IN 10_1101-2021_01_08_425864 891 31 a a DT 10_1101-2021_01_08_425864 891 32 ( ( -LRB- 10_1101-2021_01_08_425864 891 33 which which WDT 10_1101-2021_01_08_425864 891 34 was be VBD 10_1101-2021_01_08_425864 891 35 not not RB 10_1101-2021_01_08_425864 891 36 certified certify VBN 10_1101-2021_01_08_425864 891 37 by by IN 10_1101-2021_01_08_425864 891 38 peer peer NN 10_1101-2021_01_08_425864 891 39 review review NN 10_1101-2021_01_08_425864 891 40 ) ) -RRB- 10_1101-2021_01_08_425864 891 41 is be VBZ 10_1101-2021_01_08_425864 891 42 the the DT 10_1101-2021_01_08_425864 891 43 author author NN 10_1101-2021_01_08_425864 891 44 / / SYM 10_1101-2021_01_08_425864 891 45 funder funder NN 10_1101-2021_01_08_425864 891 46 , , , 10_1101-2021_01_08_425864 891 47 who who WP 10_1101-2021_01_08_425864 891 48 has have VBZ 10_1101-2021_01_08_425864 891 49 granted grant VBN 10_1101-2021_01_08_425864 891 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 891 51 a a DT 10_1101-2021_01_08_425864 891 52 license license NN 10_1101-2021_01_08_425864 891 53 to to TO 10_1101-2021_01_08_425864 891 54 display display VB 10_1101-2021_01_08_425864 891 55 the the DT 10_1101-2021_01_08_425864 891 56 preprint preprint NN 10_1101-2021_01_08_425864 891 57 in in IN 10_1101-2021_01_08_425864 891 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 891 59 . . . 10_1101-2021_01_08_425864 892 1 It -PRON- PRP 10_1101-2021_01_08_425864 892 2 is be VBZ 10_1101-2021_01_08_425864 892 3 The the DT 10_1101-2021_01_08_425864 892 4 copyright copyright NN 10_1101-2021_01_08_425864 892 5 holder holder NN 10_1101-2021_01_08_425864 892 6 for for IN 10_1101-2021_01_08_425864 892 7 this this DT 10_1101-2021_01_08_425864 892 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 892 9 version version NN 10_1101-2021_01_08_425864 892 10 posted post VBD 10_1101-2021_01_08_425864 892 11 January January NNP 10_1101-2021_01_08_425864 892 12 8 8 CD 10_1101-2021_01_08_425864 892 13 , , , 10_1101-2021_01_08_425864 892 14 2021 2021 CD 10_1101-2021_01_08_425864 892 15 . . . 10_1101-2021_01_08_425864 892 16 ; ; : 10_1101-2021_01_08_425864 892 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 892 18 : : : 10_1101-2021_01_08_425864 892 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 892 20 preprint preprint JJ 10_1101-2021_01_08_425864 892 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 892 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 892 23 .CC .CC NFP 10_1101-2021_01_08_425864 892 24 - - : 10_1101-2021_01_08_425864 892 25 BY by IN 10_1101-2021_01_08_425864 892 26 4.0 4.0 CD 10_1101-2021_01_08_425864 892 27 International international JJ 10_1101-2021_01_08_425864 892 28 licensemade licensemade NN 10_1101-2021_01_08_425864 892 29 available available JJ 10_1101-2021_01_08_425864 892 30 under under IN 10_1101-2021_01_08_425864 892 31 a a DT 10_1101-2021_01_08_425864 892 32 ( ( -LRB- 10_1101-2021_01_08_425864 892 33 which which WDT 10_1101-2021_01_08_425864 892 34 was be VBD 10_1101-2021_01_08_425864 892 35 not not RB 10_1101-2021_01_08_425864 892 36 certified certify VBN 10_1101-2021_01_08_425864 892 37 by by IN 10_1101-2021_01_08_425864 892 38 peer peer NN 10_1101-2021_01_08_425864 892 39 review review NN 10_1101-2021_01_08_425864 892 40 ) ) -RRB- 10_1101-2021_01_08_425864 892 41 is be VBZ 10_1101-2021_01_08_425864 892 42 the the DT 10_1101-2021_01_08_425864 892 43 author author NN 10_1101-2021_01_08_425864 892 44 / / SYM 10_1101-2021_01_08_425864 892 45 funder funder NN 10_1101-2021_01_08_425864 892 46 , , , 10_1101-2021_01_08_425864 892 47 who who WP 10_1101-2021_01_08_425864 892 48 has have VBZ 10_1101-2021_01_08_425864 892 49 granted grant VBN 10_1101-2021_01_08_425864 892 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 892 51 a a DT 10_1101-2021_01_08_425864 892 52 license license NN 10_1101-2021_01_08_425864 892 53 to to TO 10_1101-2021_01_08_425864 892 54 display display VB 10_1101-2021_01_08_425864 892 55 the the DT 10_1101-2021_01_08_425864 892 56 preprint preprint NN 10_1101-2021_01_08_425864 892 57 in in IN 10_1101-2021_01_08_425864 892 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 892 59 . . . 10_1101-2021_01_08_425864 893 1 It -PRON- PRP 10_1101-2021_01_08_425864 893 2 is be VBZ 10_1101-2021_01_08_425864 893 3 The the DT 10_1101-2021_01_08_425864 893 4 copyright copyright NN 10_1101-2021_01_08_425864 893 5 holder holder NN 10_1101-2021_01_08_425864 893 6 for for IN 10_1101-2021_01_08_425864 893 7 this this DT 10_1101-2021_01_08_425864 893 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 893 9 version version NN 10_1101-2021_01_08_425864 893 10 posted post VBD 10_1101-2021_01_08_425864 893 11 January January NNP 10_1101-2021_01_08_425864 893 12 8 8 CD 10_1101-2021_01_08_425864 893 13 , , , 10_1101-2021_01_08_425864 893 14 2021 2021 CD 10_1101-2021_01_08_425864 893 15 . . . 10_1101-2021_01_08_425864 893 16 ; ; : 10_1101-2021_01_08_425864 893 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 893 18 : : : 10_1101-2021_01_08_425864 893 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 893 20 preprint preprint JJ 10_1101-2021_01_08_425864 893 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 893 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 893 23 .CC .CC NFP 10_1101-2021_01_08_425864 893 24 - - : 10_1101-2021_01_08_425864 893 25 BY by IN 10_1101-2021_01_08_425864 893 26 4.0 4.0 CD 10_1101-2021_01_08_425864 893 27 International international JJ 10_1101-2021_01_08_425864 893 28 licensemade licensemade NN 10_1101-2021_01_08_425864 893 29 available available JJ 10_1101-2021_01_08_425864 893 30 under under IN 10_1101-2021_01_08_425864 893 31 a a DT 10_1101-2021_01_08_425864 893 32 ( ( -LRB- 10_1101-2021_01_08_425864 893 33 which which WDT 10_1101-2021_01_08_425864 893 34 was be VBD 10_1101-2021_01_08_425864 893 35 not not RB 10_1101-2021_01_08_425864 893 36 certified certify VBN 10_1101-2021_01_08_425864 893 37 by by IN 10_1101-2021_01_08_425864 893 38 peer peer NN 10_1101-2021_01_08_425864 893 39 review review NN 10_1101-2021_01_08_425864 893 40 ) ) -RRB- 10_1101-2021_01_08_425864 893 41 is be VBZ 10_1101-2021_01_08_425864 893 42 the the DT 10_1101-2021_01_08_425864 893 43 author author NN 10_1101-2021_01_08_425864 893 44 / / SYM 10_1101-2021_01_08_425864 893 45 funder funder NN 10_1101-2021_01_08_425864 893 46 , , , 10_1101-2021_01_08_425864 893 47 who who WP 10_1101-2021_01_08_425864 893 48 has have VBZ 10_1101-2021_01_08_425864 893 49 granted grant VBN 10_1101-2021_01_08_425864 893 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 893 51 a a DT 10_1101-2021_01_08_425864 893 52 license license NN 10_1101-2021_01_08_425864 893 53 to to TO 10_1101-2021_01_08_425864 893 54 display display VB 10_1101-2021_01_08_425864 893 55 the the DT 10_1101-2021_01_08_425864 893 56 preprint preprint NN 10_1101-2021_01_08_425864 893 57 in in IN 10_1101-2021_01_08_425864 893 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 893 59 . . . 10_1101-2021_01_08_425864 894 1 It -PRON- PRP 10_1101-2021_01_08_425864 894 2 is be VBZ 10_1101-2021_01_08_425864 894 3 The the DT 10_1101-2021_01_08_425864 894 4 copyright copyright NN 10_1101-2021_01_08_425864 894 5 holder holder NN 10_1101-2021_01_08_425864 894 6 for for IN 10_1101-2021_01_08_425864 894 7 this this DT 10_1101-2021_01_08_425864 894 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 894 9 version version NN 10_1101-2021_01_08_425864 894 10 posted post VBD 10_1101-2021_01_08_425864 894 11 January January NNP 10_1101-2021_01_08_425864 894 12 8 8 CD 10_1101-2021_01_08_425864 894 13 , , , 10_1101-2021_01_08_425864 894 14 2021 2021 CD 10_1101-2021_01_08_425864 894 15 . . . 10_1101-2021_01_08_425864 894 16 ; ; : 10_1101-2021_01_08_425864 894 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 894 18 : : : 10_1101-2021_01_08_425864 894 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 894 20 preprint preprint JJ 10_1101-2021_01_08_425864 894 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 894 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 894 23 .CC .CC NFP 10_1101-2021_01_08_425864 894 24 - - : 10_1101-2021_01_08_425864 894 25 BY by IN 10_1101-2021_01_08_425864 894 26 4.0 4.0 CD 10_1101-2021_01_08_425864 894 27 International international JJ 10_1101-2021_01_08_425864 894 28 licensemade licensemade NN 10_1101-2021_01_08_425864 894 29 available available JJ 10_1101-2021_01_08_425864 894 30 under under IN 10_1101-2021_01_08_425864 894 31 a a DT 10_1101-2021_01_08_425864 894 32 ( ( -LRB- 10_1101-2021_01_08_425864 894 33 which which WDT 10_1101-2021_01_08_425864 894 34 was be VBD 10_1101-2021_01_08_425864 894 35 not not RB 10_1101-2021_01_08_425864 894 36 certified certify VBN 10_1101-2021_01_08_425864 894 37 by by IN 10_1101-2021_01_08_425864 894 38 peer peer NN 10_1101-2021_01_08_425864 894 39 review review NN 10_1101-2021_01_08_425864 894 40 ) ) -RRB- 10_1101-2021_01_08_425864 894 41 is be VBZ 10_1101-2021_01_08_425864 894 42 the the DT 10_1101-2021_01_08_425864 894 43 author author NN 10_1101-2021_01_08_425864 894 44 / / SYM 10_1101-2021_01_08_425864 894 45 funder funder NN 10_1101-2021_01_08_425864 894 46 , , , 10_1101-2021_01_08_425864 894 47 who who WP 10_1101-2021_01_08_425864 894 48 has have VBZ 10_1101-2021_01_08_425864 894 49 granted grant VBN 10_1101-2021_01_08_425864 894 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 894 51 a a DT 10_1101-2021_01_08_425864 894 52 license license NN 10_1101-2021_01_08_425864 894 53 to to TO 10_1101-2021_01_08_425864 894 54 display display VB 10_1101-2021_01_08_425864 894 55 the the DT 10_1101-2021_01_08_425864 894 56 preprint preprint NN 10_1101-2021_01_08_425864 894 57 in in IN 10_1101-2021_01_08_425864 894 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 894 59 . . . 10_1101-2021_01_08_425864 895 1 It -PRON- PRP 10_1101-2021_01_08_425864 895 2 is be VBZ 10_1101-2021_01_08_425864 895 3 The the DT 10_1101-2021_01_08_425864 895 4 copyright copyright NN 10_1101-2021_01_08_425864 895 5 holder holder NN 10_1101-2021_01_08_425864 895 6 for for IN 10_1101-2021_01_08_425864 895 7 this this DT 10_1101-2021_01_08_425864 895 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 895 9 version version NN 10_1101-2021_01_08_425864 895 10 posted post VBD 10_1101-2021_01_08_425864 895 11 January January NNP 10_1101-2021_01_08_425864 895 12 8 8 CD 10_1101-2021_01_08_425864 895 13 , , , 10_1101-2021_01_08_425864 895 14 2021 2021 CD 10_1101-2021_01_08_425864 895 15 . . . 10_1101-2021_01_08_425864 895 16 ; ; : 10_1101-2021_01_08_425864 895 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 895 18 : : : 10_1101-2021_01_08_425864 895 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 895 20 preprint preprint JJ 10_1101-2021_01_08_425864 895 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 895 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 895 23 .CC .CC NFP 10_1101-2021_01_08_425864 895 24 - - : 10_1101-2021_01_08_425864 895 25 BY by IN 10_1101-2021_01_08_425864 895 26 4.0 4.0 CD 10_1101-2021_01_08_425864 895 27 International international JJ 10_1101-2021_01_08_425864 895 28 licensemade licensemade NN 10_1101-2021_01_08_425864 895 29 available available JJ 10_1101-2021_01_08_425864 895 30 under under IN 10_1101-2021_01_08_425864 895 31 a a DT 10_1101-2021_01_08_425864 895 32 ( ( -LRB- 10_1101-2021_01_08_425864 895 33 which which WDT 10_1101-2021_01_08_425864 895 34 was be VBD 10_1101-2021_01_08_425864 895 35 not not RB 10_1101-2021_01_08_425864 895 36 certified certify VBN 10_1101-2021_01_08_425864 895 37 by by IN 10_1101-2021_01_08_425864 895 38 peer peer NN 10_1101-2021_01_08_425864 895 39 review review NN 10_1101-2021_01_08_425864 895 40 ) ) -RRB- 10_1101-2021_01_08_425864 895 41 is be VBZ 10_1101-2021_01_08_425864 895 42 the the DT 10_1101-2021_01_08_425864 895 43 author author NN 10_1101-2021_01_08_425864 895 44 / / SYM 10_1101-2021_01_08_425864 895 45 funder funder NN 10_1101-2021_01_08_425864 895 46 , , , 10_1101-2021_01_08_425864 895 47 who who WP 10_1101-2021_01_08_425864 895 48 has have VBZ 10_1101-2021_01_08_425864 895 49 granted grant VBN 10_1101-2021_01_08_425864 895 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 895 51 a a DT 10_1101-2021_01_08_425864 895 52 license license NN 10_1101-2021_01_08_425864 895 53 to to TO 10_1101-2021_01_08_425864 895 54 display display VB 10_1101-2021_01_08_425864 895 55 the the DT 10_1101-2021_01_08_425864 895 56 preprint preprint NN 10_1101-2021_01_08_425864 895 57 in in IN 10_1101-2021_01_08_425864 895 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 895 59 . . . 10_1101-2021_01_08_425864 896 1 It -PRON- PRP 10_1101-2021_01_08_425864 896 2 is be VBZ 10_1101-2021_01_08_425864 896 3 The the DT 10_1101-2021_01_08_425864 896 4 copyright copyright NN 10_1101-2021_01_08_425864 896 5 holder holder NN 10_1101-2021_01_08_425864 896 6 for for IN 10_1101-2021_01_08_425864 896 7 this this DT 10_1101-2021_01_08_425864 896 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 896 9 version version NN 10_1101-2021_01_08_425864 896 10 posted post VBD 10_1101-2021_01_08_425864 896 11 January January NNP 10_1101-2021_01_08_425864 896 12 8 8 CD 10_1101-2021_01_08_425864 896 13 , , , 10_1101-2021_01_08_425864 896 14 2021 2021 CD 10_1101-2021_01_08_425864 896 15 . . . 10_1101-2021_01_08_425864 896 16 ; ; : 10_1101-2021_01_08_425864 896 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 896 18 : : : 10_1101-2021_01_08_425864 896 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 896 20 preprint preprint JJ 10_1101-2021_01_08_425864 896 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 896 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 896 23 .CC .CC NFP 10_1101-2021_01_08_425864 896 24 - - : 10_1101-2021_01_08_425864 896 25 BY by IN 10_1101-2021_01_08_425864 896 26 4.0 4.0 CD 10_1101-2021_01_08_425864 896 27 International international JJ 10_1101-2021_01_08_425864 896 28 licensemade licensemade NN 10_1101-2021_01_08_425864 896 29 available available JJ 10_1101-2021_01_08_425864 896 30 under under IN 10_1101-2021_01_08_425864 896 31 a a DT 10_1101-2021_01_08_425864 896 32 ( ( -LRB- 10_1101-2021_01_08_425864 896 33 which which WDT 10_1101-2021_01_08_425864 896 34 was be VBD 10_1101-2021_01_08_425864 896 35 not not RB 10_1101-2021_01_08_425864 896 36 certified certify VBN 10_1101-2021_01_08_425864 896 37 by by IN 10_1101-2021_01_08_425864 896 38 peer peer NN 10_1101-2021_01_08_425864 896 39 review review NN 10_1101-2021_01_08_425864 896 40 ) ) -RRB- 10_1101-2021_01_08_425864 896 41 is be VBZ 10_1101-2021_01_08_425864 896 42 the the DT 10_1101-2021_01_08_425864 896 43 author author NN 10_1101-2021_01_08_425864 896 44 / / SYM 10_1101-2021_01_08_425864 896 45 funder funder NN 10_1101-2021_01_08_425864 896 46 , , , 10_1101-2021_01_08_425864 896 47 who who WP 10_1101-2021_01_08_425864 896 48 has have VBZ 10_1101-2021_01_08_425864 896 49 granted grant VBN 10_1101-2021_01_08_425864 896 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 896 51 a a DT 10_1101-2021_01_08_425864 896 52 license license NN 10_1101-2021_01_08_425864 896 53 to to TO 10_1101-2021_01_08_425864 896 54 display display VB 10_1101-2021_01_08_425864 896 55 the the DT 10_1101-2021_01_08_425864 896 56 preprint preprint NN 10_1101-2021_01_08_425864 896 57 in in IN 10_1101-2021_01_08_425864 896 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 896 59 . . . 10_1101-2021_01_08_425864 897 1 It -PRON- PRP 10_1101-2021_01_08_425864 897 2 is be VBZ 10_1101-2021_01_08_425864 897 3 The the DT 10_1101-2021_01_08_425864 897 4 copyright copyright NN 10_1101-2021_01_08_425864 897 5 holder holder NN 10_1101-2021_01_08_425864 897 6 for for IN 10_1101-2021_01_08_425864 897 7 this this DT 10_1101-2021_01_08_425864 897 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 897 9 version version NN 10_1101-2021_01_08_425864 897 10 posted post VBD 10_1101-2021_01_08_425864 897 11 January January NNP 10_1101-2021_01_08_425864 897 12 8 8 CD 10_1101-2021_01_08_425864 897 13 , , , 10_1101-2021_01_08_425864 897 14 2021 2021 CD 10_1101-2021_01_08_425864 897 15 . . . 10_1101-2021_01_08_425864 897 16 ; ; : 10_1101-2021_01_08_425864 897 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 897 18 : : : 10_1101-2021_01_08_425864 897 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 897 20 preprint preprint JJ 10_1101-2021_01_08_425864 897 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 897 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 897 23 .CC .CC NFP 10_1101-2021_01_08_425864 897 24 - - : 10_1101-2021_01_08_425864 897 25 BY by IN 10_1101-2021_01_08_425864 897 26 4.0 4.0 CD 10_1101-2021_01_08_425864 897 27 International international JJ 10_1101-2021_01_08_425864 897 28 licensemade licensemade NN 10_1101-2021_01_08_425864 897 29 available available JJ 10_1101-2021_01_08_425864 897 30 under under IN 10_1101-2021_01_08_425864 897 31 a a DT 10_1101-2021_01_08_425864 897 32 ( ( -LRB- 10_1101-2021_01_08_425864 897 33 which which WDT 10_1101-2021_01_08_425864 897 34 was be VBD 10_1101-2021_01_08_425864 897 35 not not RB 10_1101-2021_01_08_425864 897 36 certified certify VBN 10_1101-2021_01_08_425864 897 37 by by IN 10_1101-2021_01_08_425864 897 38 peer peer NN 10_1101-2021_01_08_425864 897 39 review review NN 10_1101-2021_01_08_425864 897 40 ) ) -RRB- 10_1101-2021_01_08_425864 897 41 is be VBZ 10_1101-2021_01_08_425864 897 42 the the DT 10_1101-2021_01_08_425864 897 43 author author NN 10_1101-2021_01_08_425864 897 44 / / SYM 10_1101-2021_01_08_425864 897 45 funder funder NN 10_1101-2021_01_08_425864 897 46 , , , 10_1101-2021_01_08_425864 897 47 who who WP 10_1101-2021_01_08_425864 897 48 has have VBZ 10_1101-2021_01_08_425864 897 49 granted grant VBN 10_1101-2021_01_08_425864 897 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 897 51 a a DT 10_1101-2021_01_08_425864 897 52 license license NN 10_1101-2021_01_08_425864 897 53 to to TO 10_1101-2021_01_08_425864 897 54 display display VB 10_1101-2021_01_08_425864 897 55 the the DT 10_1101-2021_01_08_425864 897 56 preprint preprint NN 10_1101-2021_01_08_425864 897 57 in in IN 10_1101-2021_01_08_425864 897 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 897 59 . . . 10_1101-2021_01_08_425864 898 1 It -PRON- PRP 10_1101-2021_01_08_425864 898 2 is be VBZ 10_1101-2021_01_08_425864 898 3 The the DT 10_1101-2021_01_08_425864 898 4 copyright copyright NN 10_1101-2021_01_08_425864 898 5 holder holder NN 10_1101-2021_01_08_425864 898 6 for for IN 10_1101-2021_01_08_425864 898 7 this this DT 10_1101-2021_01_08_425864 898 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 898 9 version version NN 10_1101-2021_01_08_425864 898 10 posted post VBD 10_1101-2021_01_08_425864 898 11 January January NNP 10_1101-2021_01_08_425864 898 12 8 8 CD 10_1101-2021_01_08_425864 898 13 , , , 10_1101-2021_01_08_425864 898 14 2021 2021 CD 10_1101-2021_01_08_425864 898 15 . . . 10_1101-2021_01_08_425864 898 16 ; ; : 10_1101-2021_01_08_425864 898 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 898 18 : : : 10_1101-2021_01_08_425864 898 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 898 20 preprint preprint JJ 10_1101-2021_01_08_425864 898 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 898 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD 10_1101-2021_01_08_425864 898 23 .CC .CC NFP 10_1101-2021_01_08_425864 898 24 - - : 10_1101-2021_01_08_425864 898 25 BY by IN 10_1101-2021_01_08_425864 898 26 4.0 4.0 CD 10_1101-2021_01_08_425864 898 27 International international JJ 10_1101-2021_01_08_425864 898 28 licensemade licensemade NN 10_1101-2021_01_08_425864 898 29 available available JJ 10_1101-2021_01_08_425864 898 30 under under IN 10_1101-2021_01_08_425864 898 31 a a DT 10_1101-2021_01_08_425864 898 32 ( ( -LRB- 10_1101-2021_01_08_425864 898 33 which which WDT 10_1101-2021_01_08_425864 898 34 was be VBD 10_1101-2021_01_08_425864 898 35 not not RB 10_1101-2021_01_08_425864 898 36 certified certify VBN 10_1101-2021_01_08_425864 898 37 by by IN 10_1101-2021_01_08_425864 898 38 peer peer NN 10_1101-2021_01_08_425864 898 39 review review NN 10_1101-2021_01_08_425864 898 40 ) ) -RRB- 10_1101-2021_01_08_425864 898 41 is be VBZ 10_1101-2021_01_08_425864 898 42 the the DT 10_1101-2021_01_08_425864 898 43 author author NN 10_1101-2021_01_08_425864 898 44 / / SYM 10_1101-2021_01_08_425864 898 45 funder funder NN 10_1101-2021_01_08_425864 898 46 , , , 10_1101-2021_01_08_425864 898 47 who who WP 10_1101-2021_01_08_425864 898 48 has have VBZ 10_1101-2021_01_08_425864 898 49 granted grant VBN 10_1101-2021_01_08_425864 898 50 bioRxiv biorxiv IN 10_1101-2021_01_08_425864 898 51 a a DT 10_1101-2021_01_08_425864 898 52 license license NN 10_1101-2021_01_08_425864 898 53 to to TO 10_1101-2021_01_08_425864 898 54 display display VB 10_1101-2021_01_08_425864 898 55 the the DT 10_1101-2021_01_08_425864 898 56 preprint preprint NN 10_1101-2021_01_08_425864 898 57 in in IN 10_1101-2021_01_08_425864 898 58 perpetuity perpetuity NN 10_1101-2021_01_08_425864 898 59 . . . 10_1101-2021_01_08_425864 899 1 It -PRON- PRP 10_1101-2021_01_08_425864 899 2 is be VBZ 10_1101-2021_01_08_425864 899 3 The the DT 10_1101-2021_01_08_425864 899 4 copyright copyright NN 10_1101-2021_01_08_425864 899 5 holder holder NN 10_1101-2021_01_08_425864 899 6 for for IN 10_1101-2021_01_08_425864 899 7 this this DT 10_1101-2021_01_08_425864 899 8 preprintthis preprintthis NN 10_1101-2021_01_08_425864 899 9 version version NN 10_1101-2021_01_08_425864 899 10 posted post VBD 10_1101-2021_01_08_425864 899 11 January January NNP 10_1101-2021_01_08_425864 899 12 8 8 CD 10_1101-2021_01_08_425864 899 13 , , , 10_1101-2021_01_08_425864 899 14 2021 2021 CD 10_1101-2021_01_08_425864 899 15 . . . 10_1101-2021_01_08_425864 899 16 ; ; : 10_1101-2021_01_08_425864 899 17 https://doi.org/10.1101/2021.01.08.425864doi https://doi.org/10.1101/2021.01.08.425864doi ADD 10_1101-2021_01_08_425864 899 18 : : : 10_1101-2021_01_08_425864 899 19 bioRxiv biorxiv VB 10_1101-2021_01_08_425864 899 20 preprint preprint NN 10_1101-2021_01_08_425864 899 21 https://doi.org/10.1101/2021.01.08.425864 https://doi.org/10.1101/2021.01.08.425864 ADD 10_1101-2021_01_08_425864 899 22 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ ADD