id sid tid token lemma pos 10_1101-2021_01_07_425737 1 1 Isolation isolation NN 10_1101-2021_01_07_425737 1 2 of of IN 10_1101-2021_01_07_425737 1 3 the the DT 10_1101-2021_01_07_425737 1 4 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 1 5 aphidicola aphidicola NN 10_1101-2021_01_07_425737 1 6 flagellum flagellum NN 10_1101-2021_01_07_425737 1 7 basal basal NN 10_1101-2021_01_07_425737 1 8 body body NN 10_1101-2021_01_07_425737 1 9 from from IN 10_1101-2021_01_07_425737 1 10 the the DT 10_1101-2021_01_07_425737 1 11 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 1 12 membrane membrane NN 10_1101-2021_01_07_425737 1 13 Isolation isolation NN 10_1101-2021_01_07_425737 1 14 of of IN 10_1101-2021_01_07_425737 1 15 the the DT 10_1101-2021_01_07_425737 1 16 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 1 17 aphidicola aphidicola NN 10_1101-2021_01_07_425737 1 18 flagellum flagellum NN 10_1101-2021_01_07_425737 1 19 basal basal NN 10_1101-2021_01_07_425737 1 20 body body NN 10_1101-2021_01_07_425737 1 21 from from IN 10_1101-2021_01_07_425737 1 22 the the DT 10_1101-2021_01_07_425737 1 23 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 1 24 membrane membrane NN 10_1101-2021_01_07_425737 1 25 Matthew Matthew NNP 10_1101-2021_01_07_425737 1 26 J. J. NNP 10_1101-2021_01_07_425737 1 27 Schepers1 Schepers1 NNP 10_1101-2021_01_07_425737 1 28 , , , 10_1101-2021_01_07_425737 1 29 James James NNP 10_1101-2021_01_07_425737 1 30 N. N. NNP 10_1101-2021_01_07_425737 1 31 Yelland1 Yelland1 NNP 10_1101-2021_01_07_425737 1 32 , , , 10_1101-2021_01_07_425737 1 33 Nancy Nancy NNP 10_1101-2021_01_07_425737 1 34 A. A. NNP 10_1101-2021_01_07_425737 1 35 Moran2 Moran2 NNP 10_1101-2021_01_07_425737 1 36 * * NFP 10_1101-2021_01_07_425737 1 37 , , , 10_1101-2021_01_07_425737 1 38 David David NNP 10_1101-2021_01_07_425737 1 39 W. W. NNP 10_1101-2021_01_07_425737 1 40 Taylor1,3 taylor1,3 CD 10_1101-2021_01_07_425737 1 41 - - HYPH 10_1101-2021_01_07_425737 1 42 5 5 CD 10_1101-2021_01_07_425737 1 43 * * NFP 10_1101-2021_01_07_425737 1 44 1Institute 1institute CD 10_1101-2021_01_07_425737 1 45 for for IN 10_1101-2021_01_07_425737 1 46 Cell Cell NNP 10_1101-2021_01_07_425737 1 47 and and CC 10_1101-2021_01_07_425737 1 48 Molecular Molecular NNP 10_1101-2021_01_07_425737 1 49 Biology Biology NNP 10_1101-2021_01_07_425737 1 50 , , , 10_1101-2021_01_07_425737 1 51 University University NNP 10_1101-2021_01_07_425737 1 52 of of IN 10_1101-2021_01_07_425737 1 53 Texas Texas NNP 10_1101-2021_01_07_425737 1 54 at at IN 10_1101-2021_01_07_425737 1 55 Austin Austin NNP 10_1101-2021_01_07_425737 1 56 , , , 10_1101-2021_01_07_425737 1 57 Austin Austin NNP 10_1101-2021_01_07_425737 1 58 , , , 10_1101-2021_01_07_425737 1 59 TX TX NNP 10_1101-2021_01_07_425737 1 60 , , , 10_1101-2021_01_07_425737 1 61 78712 78712 CD 10_1101-2021_01_07_425737 1 62 2Department 2department CD 10_1101-2021_01_07_425737 1 63 of of IN 10_1101-2021_01_07_425737 1 64 Integrative Integrative NNP 10_1101-2021_01_07_425737 1 65 Biology Biology NNP 10_1101-2021_01_07_425737 1 66 , , , 10_1101-2021_01_07_425737 1 67 University University NNP 10_1101-2021_01_07_425737 1 68 of of IN 10_1101-2021_01_07_425737 1 69 Texas Texas NNP 10_1101-2021_01_07_425737 1 70 at at IN 10_1101-2021_01_07_425737 1 71 Austin Austin NNP 10_1101-2021_01_07_425737 1 72 , , , 10_1101-2021_01_07_425737 1 73 Austin Austin NNP 10_1101-2021_01_07_425737 1 74 , , , 10_1101-2021_01_07_425737 1 75 TX TX NNP 10_1101-2021_01_07_425737 1 76 , , , 10_1101-2021_01_07_425737 1 77 78712 78712 CD 10_1101-2021_01_07_425737 1 78 3Departmnet 3Departmnet NNP 10_1101-2021_01_07_425737 1 79 of of IN 10_1101-2021_01_07_425737 1 80 Molecular Molecular NNP 10_1101-2021_01_07_425737 1 81 Biosciences Biosciences NNPS 10_1101-2021_01_07_425737 1 82 , , , 10_1101-2021_01_07_425737 1 83 University University NNP 10_1101-2021_01_07_425737 1 84 of of IN 10_1101-2021_01_07_425737 1 85 Texas Texas NNP 10_1101-2021_01_07_425737 1 86 at at IN 10_1101-2021_01_07_425737 1 87 Austin Austin NNP 10_1101-2021_01_07_425737 1 88 , , , 10_1101-2021_01_07_425737 1 89 Austin Austin NNP 10_1101-2021_01_07_425737 1 90 , , , 10_1101-2021_01_07_425737 1 91 TX TX NNP 10_1101-2021_01_07_425737 1 92 , , , 10_1101-2021_01_07_425737 1 93 78712 78712 CD 10_1101-2021_01_07_425737 1 94 4Center 4Center NNP 10_1101-2021_01_07_425737 1 95 for for IN 10_1101-2021_01_07_425737 1 96 Systems Systems NNPS 10_1101-2021_01_07_425737 1 97 and and CC 10_1101-2021_01_07_425737 1 98 Synthetic Synthetic NNP 10_1101-2021_01_07_425737 1 99 Biology Biology NNP 10_1101-2021_01_07_425737 1 100 , , , 10_1101-2021_01_07_425737 1 101 University University NNP 10_1101-2021_01_07_425737 1 102 of of IN 10_1101-2021_01_07_425737 1 103 Texas Texas NNP 10_1101-2021_01_07_425737 1 104 at at IN 10_1101-2021_01_07_425737 1 105 Austin Austin NNP 10_1101-2021_01_07_425737 1 106 , , , 10_1101-2021_01_07_425737 1 107 Austin Austin NNP 10_1101-2021_01_07_425737 1 108 , , , 10_1101-2021_01_07_425737 1 109 TX TX NNP 10_1101-2021_01_07_425737 1 110 , , , 10_1101-2021_01_07_425737 1 111 78712 78712 CD 10_1101-2021_01_07_425737 1 112 5LIVESTRONG 5LIVESTRONG NNP 10_1101-2021_01_07_425737 1 113 Cancer Cancer NNP 10_1101-2021_01_07_425737 1 114 Institute Institute NNP 10_1101-2021_01_07_425737 1 115 , , , 10_1101-2021_01_07_425737 1 116 Dell Dell NNP 10_1101-2021_01_07_425737 1 117 Medical Medical NNP 10_1101-2021_01_07_425737 1 118 School School NNP 10_1101-2021_01_07_425737 1 119 , , , 10_1101-2021_01_07_425737 1 120 Austin Austin NNP 10_1101-2021_01_07_425737 1 121 , , , 10_1101-2021_01_07_425737 1 122 TX TX NNP 10_1101-2021_01_07_425737 1 123 , , , 10_1101-2021_01_07_425737 1 124 78712 78712 CD 10_1101-2021_01_07_425737 1 125 * * NFP 10_1101-2021_01_07_425737 1 126 Correspondence Correspondence NNP 10_1101-2021_01_07_425737 1 127 to to TO 10_1101-2021_01_07_425737 1 128 : : : 10_1101-2021_01_07_425737 1 129 dtaylor@utexas.edu dtaylor@utexas.edu NNP 10_1101-2021_01_07_425737 1 130 ( ( -LRB- 10_1101-2021_01_07_425737 1 131 D.W.T. D.W.T. NNP 10_1101-2021_01_07_425737 2 1 ) ) -RRB- 10_1101-2021_01_07_425737 2 2 ; ; : 10_1101-2021_01_07_425737 2 3 nancy.moran@austin.utexas.edu nancy.moran@austin.utexas.edu NNP 10_1101-2021_01_07_425737 2 4 ( ( -LRB- 10_1101-2021_01_07_425737 2 5 N.A.M. N.A.M. NNP 10_1101-2021_01_07_425737 2 6 ) ) -RRB- 10_1101-2021_01_07_425737 3 1 ( ( -LRB- 10_1101-2021_01_07_425737 3 2 which which WDT 10_1101-2021_01_07_425737 3 3 was be VBD 10_1101-2021_01_07_425737 3 4 not not RB 10_1101-2021_01_07_425737 3 5 certified certify VBN 10_1101-2021_01_07_425737 3 6 by by IN 10_1101-2021_01_07_425737 3 7 peer peer NN 10_1101-2021_01_07_425737 3 8 review review NN 10_1101-2021_01_07_425737 3 9 ) ) -RRB- 10_1101-2021_01_07_425737 3 10 is be VBZ 10_1101-2021_01_07_425737 3 11 the the DT 10_1101-2021_01_07_425737 3 12 author author NN 10_1101-2021_01_07_425737 3 13 / / SYM 10_1101-2021_01_07_425737 3 14 funder funder NN 10_1101-2021_01_07_425737 3 15 . . . 10_1101-2021_01_07_425737 4 1 All all DT 10_1101-2021_01_07_425737 4 2 rights right NNS 10_1101-2021_01_07_425737 4 3 reserved reserve VBD 10_1101-2021_01_07_425737 4 4 . . . 10_1101-2021_01_07_425737 5 1 No no DT 10_1101-2021_01_07_425737 5 2 reuse reuse NN 10_1101-2021_01_07_425737 5 3 allowed allow VBN 10_1101-2021_01_07_425737 5 4 without without IN 10_1101-2021_01_07_425737 5 5 permission permission NN 10_1101-2021_01_07_425737 5 6 . . . 10_1101-2021_01_07_425737 6 1 The the DT 10_1101-2021_01_07_425737 6 2 copyright copyright NN 10_1101-2021_01_07_425737 6 3 holder holder NN 10_1101-2021_01_07_425737 6 4 for for IN 10_1101-2021_01_07_425737 6 5 this this DT 10_1101-2021_01_07_425737 6 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 6 7 version version NN 10_1101-2021_01_07_425737 6 8 posted post VBD 10_1101-2021_01_07_425737 6 9 January January NNP 10_1101-2021_01_07_425737 6 10 8 8 CD 10_1101-2021_01_07_425737 6 11 , , , 10_1101-2021_01_07_425737 6 12 2021 2021 CD 10_1101-2021_01_07_425737 6 13 . . . 10_1101-2021_01_07_425737 6 14 ; ; : 10_1101-2021_01_07_425737 6 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 6 16 : : : 10_1101-2021_01_07_425737 6 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 6 18 preprint preprint NN 10_1101-2021_01_07_425737 6 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 6 20 Abstract Abstract NNP 10_1101-2021_01_07_425737 6 21 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 6 22 aphidicola aphidicola NN 10_1101-2021_01_07_425737 6 23 is be VBZ 10_1101-2021_01_07_425737 6 24 an an DT 10_1101-2021_01_07_425737 6 25 intracellular intracellular JJ 10_1101-2021_01_07_425737 6 26 bacterial bacterial JJ 10_1101-2021_01_07_425737 6 27 symbiont symbiont NN 10_1101-2021_01_07_425737 6 28 of of IN 10_1101-2021_01_07_425737 6 29 aphids aphid NNS 10_1101-2021_01_07_425737 6 30 and and CC 10_1101-2021_01_07_425737 6 31 maintains maintain VBZ 10_1101-2021_01_07_425737 6 32 a a DT 10_1101-2021_01_07_425737 6 33 small small JJ 10_1101-2021_01_07_425737 6 34 genome genome NN 10_1101-2021_01_07_425737 6 35 of of IN 10_1101-2021_01_07_425737 6 36 only only RB 10_1101-2021_01_07_425737 6 37 600 600 CD 10_1101-2021_01_07_425737 6 38 kbps kbp NNS 10_1101-2021_01_07_425737 6 39 . . . 10_1101-2021_01_07_425737 7 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 7 2 is be VBZ 10_1101-2021_01_07_425737 7 3 thought think VBN 10_1101-2021_01_07_425737 7 4 to to TO 10_1101-2021_01_07_425737 7 5 maintain maintain VB 10_1101-2021_01_07_425737 7 6 only only JJ 10_1101-2021_01_07_425737 7 7 genes gene NNS 10_1101-2021_01_07_425737 7 8 relevant relevant JJ 10_1101-2021_01_07_425737 7 9 to to IN 10_1101-2021_01_07_425737 7 10 the the DT 10_1101-2021_01_07_425737 7 11 symbiosis symbiosis NN 10_1101-2021_01_07_425737 7 12 with with IN 10_1101-2021_01_07_425737 7 13 its -PRON- PRP$ 10_1101-2021_01_07_425737 7 14 aphid aphid NNP 10_1101-2021_01_07_425737 7 15 host host NN 10_1101-2021_01_07_425737 7 16 . . . 10_1101-2021_01_07_425737 8 1 Curiously Curiously NNP 10_1101-2021_01_07_425737 8 2 , , , 10_1101-2021_01_07_425737 8 3 the the DT 10_1101-2021_01_07_425737 8 4 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 8 5 genome genome NN 10_1101-2021_01_07_425737 8 6 contains contain VBZ 10_1101-2021_01_07_425737 8 7 gene gene NN 10_1101-2021_01_07_425737 8 8 clusters cluster NNS 10_1101-2021_01_07_425737 8 9 coding code VBG 10_1101-2021_01_07_425737 8 10 for for IN 10_1101-2021_01_07_425737 8 11 flagellum flagellum JJ 10_1101-2021_01_07_425737 8 12 basal basal NNP 10_1101-2021_01_07_425737 8 13 body body NNP 10_1101-2021_01_07_425737 8 14 structural structural JJ 10_1101-2021_01_07_425737 8 15 proteins protein NNS 10_1101-2021_01_07_425737 8 16 and and CC 10_1101-2021_01_07_425737 8 17 for for IN 10_1101-2021_01_07_425737 8 18 flagellum flagellum NN 10_1101-2021_01_07_425737 8 19 type type NN 10_1101-2021_01_07_425737 8 20 III iii CD 10_1101-2021_01_07_425737 8 21 export export NN 10_1101-2021_01_07_425737 8 22 machinery machinery NN 10_1101-2021_01_07_425737 8 23 . . . 10_1101-2021_01_07_425737 9 1 These these DT 10_1101-2021_01_07_425737 9 2 structures structure NNS 10_1101-2021_01_07_425737 9 3 have have VBP 10_1101-2021_01_07_425737 9 4 been be VBN 10_1101-2021_01_07_425737 9 5 shown show VBN 10_1101-2021_01_07_425737 9 6 to to TO 10_1101-2021_01_07_425737 9 7 be be VB 10_1101-2021_01_07_425737 9 8 highly highly RB 10_1101-2021_01_07_425737 9 9 expressed express VBN 10_1101-2021_01_07_425737 9 10 and and CC 10_1101-2021_01_07_425737 9 11 present present JJ 10_1101-2021_01_07_425737 9 12 in in IN 10_1101-2021_01_07_425737 9 13 large large JJ 10_1101-2021_01_07_425737 9 14 numbers number NNS 10_1101-2021_01_07_425737 9 15 on on IN 10_1101-2021_01_07_425737 9 16 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 9 17 cells cell NNS 10_1101-2021_01_07_425737 9 18 . . . 10_1101-2021_01_07_425737 10 1 No no DT 10_1101-2021_01_07_425737 10 2 recognizable recognizable JJ 10_1101-2021_01_07_425737 10 3 pathogenicity pathogenicity NN 10_1101-2021_01_07_425737 10 4 factors factor NNS 10_1101-2021_01_07_425737 10 5 or or CC 10_1101-2021_01_07_425737 10 6 secreted secrete VBN 10_1101-2021_01_07_425737 10 7 proteins protein NNS 10_1101-2021_01_07_425737 10 8 have have VBP 10_1101-2021_01_07_425737 10 9 been be VBN 10_1101-2021_01_07_425737 10 10 identified identify VBN 10_1101-2021_01_07_425737 10 11 in in IN 10_1101-2021_01_07_425737 10 12 the the DT 10_1101-2021_01_07_425737 10 13 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 10 14 genome genome NN 10_1101-2021_01_07_425737 10 15 , , , 10_1101-2021_01_07_425737 10 16 and and CC 10_1101-2021_01_07_425737 10 17 the the DT 10_1101-2021_01_07_425737 10 18 relevance relevance NN 10_1101-2021_01_07_425737 10 19 of of IN 10_1101-2021_01_07_425737 10 20 this this DT 10_1101-2021_01_07_425737 10 21 protein protein NN 10_1101-2021_01_07_425737 10 22 complex complex NN 10_1101-2021_01_07_425737 10 23 to to IN 10_1101-2021_01_07_425737 10 24 the the DT 10_1101-2021_01_07_425737 10 25 symbiosis symbiosis NN 10_1101-2021_01_07_425737 10 26 is be VBZ 10_1101-2021_01_07_425737 10 27 unknown unknown JJ 10_1101-2021_01_07_425737 10 28 . . . 10_1101-2021_01_07_425737 11 1 Here here RB 10_1101-2021_01_07_425737 11 2 , , , 10_1101-2021_01_07_425737 11 3 we -PRON- PRP 10_1101-2021_01_07_425737 11 4 show show VBP 10_1101-2021_01_07_425737 11 5 isolation isolation NN 10_1101-2021_01_07_425737 11 6 of of IN 10_1101-2021_01_07_425737 11 7 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 11 8 flagella flagella NN 10_1101-2021_01_07_425737 11 9 from from IN 10_1101-2021_01_07_425737 11 10 the the DT 10_1101-2021_01_07_425737 11 11 cellular cellular JJ 10_1101-2021_01_07_425737 11 12 membrane membrane NN 10_1101-2021_01_07_425737 11 13 of of IN 10_1101-2021_01_07_425737 11 14 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 11 15 , , , 10_1101-2021_01_07_425737 11 16 confirming confirm VBG 10_1101-2021_01_07_425737 11 17 the the DT 10_1101-2021_01_07_425737 11 18 enrichment enrichment NN 10_1101-2021_01_07_425737 11 19 of of IN 10_1101-2021_01_07_425737 11 20 flagellum flagellum JJ 10_1101-2021_01_07_425737 11 21 proteins protein NNS 10_1101-2021_01_07_425737 11 22 relative relative JJ 10_1101-2021_01_07_425737 11 23 to to IN 10_1101-2021_01_07_425737 11 24 other other JJ 10_1101-2021_01_07_425737 11 25 proteins protein NNS 10_1101-2021_01_07_425737 11 26 in in IN 10_1101-2021_01_07_425737 11 27 the the DT 10_1101-2021_01_07_425737 11 28 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 11 29 proteome proteome NN 10_1101-2021_01_07_425737 11 30 . . . 10_1101-2021_01_07_425737 12 1 This this DT 10_1101-2021_01_07_425737 12 2 will will MD 10_1101-2021_01_07_425737 12 3 facilitate facilitate VB 10_1101-2021_01_07_425737 12 4 studies study NNS 10_1101-2021_01_07_425737 12 5 of of IN 10_1101-2021_01_07_425737 12 6 the the DT 10_1101-2021_01_07_425737 12 7 structure structure NN 10_1101-2021_01_07_425737 12 8 and and CC 10_1101-2021_01_07_425737 12 9 function function NN 10_1101-2021_01_07_425737 12 10 of of IN 10_1101-2021_01_07_425737 12 11 the the DT 10_1101-2021_01_07_425737 12 12 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 12 13 flagellum flagellum NN 10_1101-2021_01_07_425737 12 14 structure structure NN 10_1101-2021_01_07_425737 12 15 , , , 10_1101-2021_01_07_425737 12 16 and and CC 10_1101-2021_01_07_425737 12 17 its -PRON- PRP$ 10_1101-2021_01_07_425737 12 18 role role NN 10_1101-2021_01_07_425737 12 19 in in IN 10_1101-2021_01_07_425737 12 20 this this DT 10_1101-2021_01_07_425737 12 21 model model NN 10_1101-2021_01_07_425737 12 22 symbiosis symbiosis NN 10_1101-2021_01_07_425737 12 23 . . . 10_1101-2021_01_07_425737 13 1 ( ( -LRB- 10_1101-2021_01_07_425737 13 2 which which WDT 10_1101-2021_01_07_425737 13 3 was be VBD 10_1101-2021_01_07_425737 13 4 not not RB 10_1101-2021_01_07_425737 13 5 certified certify VBN 10_1101-2021_01_07_425737 13 6 by by IN 10_1101-2021_01_07_425737 13 7 peer peer NN 10_1101-2021_01_07_425737 13 8 review review NN 10_1101-2021_01_07_425737 13 9 ) ) -RRB- 10_1101-2021_01_07_425737 13 10 is be VBZ 10_1101-2021_01_07_425737 13 11 the the DT 10_1101-2021_01_07_425737 13 12 author author NN 10_1101-2021_01_07_425737 13 13 / / SYM 10_1101-2021_01_07_425737 13 14 funder funder NN 10_1101-2021_01_07_425737 13 15 . . . 10_1101-2021_01_07_425737 14 1 All all DT 10_1101-2021_01_07_425737 14 2 rights right NNS 10_1101-2021_01_07_425737 14 3 reserved reserve VBD 10_1101-2021_01_07_425737 14 4 . . . 10_1101-2021_01_07_425737 15 1 No no DT 10_1101-2021_01_07_425737 15 2 reuse reuse NN 10_1101-2021_01_07_425737 15 3 allowed allow VBN 10_1101-2021_01_07_425737 15 4 without without IN 10_1101-2021_01_07_425737 15 5 permission permission NN 10_1101-2021_01_07_425737 15 6 . . . 10_1101-2021_01_07_425737 16 1 The the DT 10_1101-2021_01_07_425737 16 2 copyright copyright NN 10_1101-2021_01_07_425737 16 3 holder holder NN 10_1101-2021_01_07_425737 16 4 for for IN 10_1101-2021_01_07_425737 16 5 this this DT 10_1101-2021_01_07_425737 16 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 16 7 version version NN 10_1101-2021_01_07_425737 16 8 posted post VBD 10_1101-2021_01_07_425737 16 9 January January NNP 10_1101-2021_01_07_425737 16 10 8 8 CD 10_1101-2021_01_07_425737 16 11 , , , 10_1101-2021_01_07_425737 16 12 2021 2021 CD 10_1101-2021_01_07_425737 16 13 . . . 10_1101-2021_01_07_425737 16 14 ; ; : 10_1101-2021_01_07_425737 16 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 16 16 : : : 10_1101-2021_01_07_425737 16 17 bioRxiv biorxiv VB 10_1101-2021_01_07_425737 16 18 preprint preprint NN 10_1101-2021_01_07_425737 16 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNS 10_1101-2021_01_07_425737 16 20 Introduction Introduction NNP 10_1101-2021_01_07_425737 16 21 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 16 22 aphidicola aphidicola NN 10_1101-2021_01_07_425737 16 23 is be VBZ 10_1101-2021_01_07_425737 16 24 an an DT 10_1101-2021_01_07_425737 16 25 obligate obligate NN 10_1101-2021_01_07_425737 16 26 endosymbiont endosymbiont NN 10_1101-2021_01_07_425737 16 27 of of IN 10_1101-2021_01_07_425737 16 28 aphid aphid NNP 10_1101-2021_01_07_425737 16 29 species specie NNS 10_1101-2021_01_07_425737 16 30 worldwide1 worldwide1 NNP 10_1101-2021_01_07_425737 16 31 and and CC 10_1101-2021_01_07_425737 16 32 is be VBZ 10_1101-2021_01_07_425737 16 33 a a DT 10_1101-2021_01_07_425737 16 34 model model NN 10_1101-2021_01_07_425737 16 35 for for IN 10_1101-2021_01_07_425737 16 36 bacterial bacterial JJ 10_1101-2021_01_07_425737 16 37 genome genome NN 10_1101-2021_01_07_425737 16 38 reduction reduction NN 10_1101-2021_01_07_425737 16 39 , , , 10_1101-2021_01_07_425737 16 40 maintaining maintain VBG 10_1101-2021_01_07_425737 16 41 one one CD 10_1101-2021_01_07_425737 16 42 of of IN 10_1101-2021_01_07_425737 16 43 the the DT 10_1101-2021_01_07_425737 16 44 smallest small JJS 10_1101-2021_01_07_425737 16 45 genomes genome NNS 10_1101-2021_01_07_425737 16 46 yet yet CC 10_1101-2021_01_07_425737 16 47 discovered discover VBN 10_1101-2021_01_07_425737 16 48 , , , 10_1101-2021_01_07_425737 16 49 only only RB 10_1101-2021_01_07_425737 16 50 600 600 CD 10_1101-2021_01_07_425737 16 51 kbps2,3 kbps2,3 CD 10_1101-2021_01_07_425737 16 52 . . . 10_1101-2021_01_07_425737 17 1 Though though IN 10_1101-2021_01_07_425737 17 2 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 17 3 has have VBZ 10_1101-2021_01_07_425737 17 4 lost lose VBN 10_1101-2021_01_07_425737 17 5 genes gene NNS 10_1101-2021_01_07_425737 17 6 not not RB 10_1101-2021_01_07_425737 17 7 essential essential JJ 10_1101-2021_01_07_425737 17 8 for for IN 10_1101-2021_01_07_425737 17 9 its -PRON- PRP$ 10_1101-2021_01_07_425737 17 10 symbiotic symbiotic JJ 10_1101-2021_01_07_425737 17 11 lifestyle2,4,5 lifestyle2,4,5 NNS 10_1101-2021_01_07_425737 17 12 it -PRON- PRP 10_1101-2021_01_07_425737 17 13 retains retain VBZ 10_1101-2021_01_07_425737 17 14 genes gene NNS 10_1101-2021_01_07_425737 17 15 associated associate VBN 10_1101-2021_01_07_425737 17 16 with with IN 10_1101-2021_01_07_425737 17 17 amino amino JJ 10_1101-2021_01_07_425737 17 18 acid acid NN 10_1101-2021_01_07_425737 17 19 biosynthesis biosynthesis NN 10_1101-2021_01_07_425737 17 20 , , , 10_1101-2021_01_07_425737 17 21 reflecting reflect VBG 10_1101-2021_01_07_425737 17 22 its -PRON- PRP$ 10_1101-2021_01_07_425737 17 23 participation participation NN 10_1101-2021_01_07_425737 17 24 in in IN 10_1101-2021_01_07_425737 17 25 a a DT 10_1101-2021_01_07_425737 17 26 nutritional nutritional JJ 10_1101-2021_01_07_425737 17 27 symbiosis2,6,7 symbiosis2,6,7 NN 10_1101-2021_01_07_425737 17 28 . . . 10_1101-2021_01_07_425737 18 1 Though though IN 10_1101-2021_01_07_425737 18 2 the the DT 10_1101-2021_01_07_425737 18 3 exchange exchange NN 10_1101-2021_01_07_425737 18 4 of of IN 10_1101-2021_01_07_425737 18 5 amino amino JJ 10_1101-2021_01_07_425737 18 6 acids acid NNS 10_1101-2021_01_07_425737 18 7 and and CC 10_1101-2021_01_07_425737 18 8 vitamins vitamin NNS 10_1101-2021_01_07_425737 18 9 between between IN 10_1101-2021_01_07_425737 18 10 the the DT 10_1101-2021_01_07_425737 18 11 aphid aphid NNP 10_1101-2021_01_07_425737 18 12 host host NN 10_1101-2021_01_07_425737 18 13 and and CC 10_1101-2021_01_07_425737 18 14 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 18 15 has have VBZ 10_1101-2021_01_07_425737 18 16 been be VBN 10_1101-2021_01_07_425737 18 17 well well RB 10_1101-2021_01_07_425737 18 18 - - HYPH 10_1101-2021_01_07_425737 18 19 documented6,8,9 documented6,8,9 NNP 10_1101-2021_01_07_425737 18 20 , , , 10_1101-2021_01_07_425737 18 21 the the DT 10_1101-2021_01_07_425737 18 22 molecular molecular JJ 10_1101-2021_01_07_425737 18 23 mechanism mechanism NN 10_1101-2021_01_07_425737 18 24 for for IN 10_1101-2021_01_07_425737 18 25 how how WRB 10_1101-2021_01_07_425737 18 26 these these DT 10_1101-2021_01_07_425737 18 27 metabolites metabolite NNS 10_1101-2021_01_07_425737 18 28 cross cross VBP 10_1101-2021_01_07_425737 18 29 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 18 30 membranes membrane NNS 10_1101-2021_01_07_425737 18 31 is be VBZ 10_1101-2021_01_07_425737 18 32 unknown unknown JJ 10_1101-2021_01_07_425737 18 33 : : : 10_1101-2021_01_07_425737 18 34 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 18 35 maintains maintain VBZ 10_1101-2021_01_07_425737 18 36 a a DT 10_1101-2021_01_07_425737 18 37 small small JJ 10_1101-2021_01_07_425737 18 38 number number NN 10_1101-2021_01_07_425737 18 39 of of IN 10_1101-2021_01_07_425737 18 40 genes gene NNS 10_1101-2021_01_07_425737 18 41 coding code VBG 10_1101-2021_01_07_425737 18 42 for for IN 10_1101-2021_01_07_425737 18 43 membrane membrane NN 10_1101-2021_01_07_425737 18 44 transport transport NN 10_1101-2021_01_07_425737 18 45 proteins protein NNS 10_1101-2021_01_07_425737 18 46 , , , 10_1101-2021_01_07_425737 18 47 most most JJS 10_1101-2021_01_07_425737 18 48 of of IN 10_1101-2021_01_07_425737 18 49 which which WDT 10_1101-2021_01_07_425737 18 50 are be VBP 10_1101-2021_01_07_425737 18 51 located locate VBN 10_1101-2021_01_07_425737 18 52 at at IN 10_1101-2021_01_07_425737 18 53 the the DT 10_1101-2021_01_07_425737 18 54 inner inner JJ 10_1101-2021_01_07_425737 18 55 membrane2,10 membrane2,10 NNP 10_1101-2021_01_07_425737 18 56 . . . 10_1101-2021_01_07_425737 19 1 The the DT 10_1101-2021_01_07_425737 19 2 permeability permeability NN 10_1101-2021_01_07_425737 19 3 of of IN 10_1101-2021_01_07_425737 19 4 the the DT 10_1101-2021_01_07_425737 19 5 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 19 6 outer outer JJ 10_1101-2021_01_07_425737 19 7 membrane membrane NN 10_1101-2021_01_07_425737 19 8 remains remain VBZ 10_1101-2021_01_07_425737 19 9 a a DT 10_1101-2021_01_07_425737 19 10 mystery mystery NN 10_1101-2021_01_07_425737 19 11 , , , 10_1101-2021_01_07_425737 19 12 considering consider VBG 10_1101-2021_01_07_425737 19 13 the the DT 10_1101-2021_01_07_425737 19 14 paucity paucity NN 10_1101-2021_01_07_425737 19 15 of of IN 10_1101-2021_01_07_425737 19 16 annotated annotate VBN 10_1101-2021_01_07_425737 19 17 transporter transporter NN 10_1101-2021_01_07_425737 19 18 genes gene NNS 10_1101-2021_01_07_425737 19 19 in in IN 10_1101-2021_01_07_425737 19 20 sequenced sequenced JJ 10_1101-2021_01_07_425737 19 21 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 19 22 genomes genome NNS 10_1101-2021_01_07_425737 19 23 . . . 10_1101-2021_01_07_425737 20 1 Genes gene NNS 10_1101-2021_01_07_425737 20 2 coding code VBG 10_1101-2021_01_07_425737 20 3 for for IN 10_1101-2021_01_07_425737 20 4 proteins protein NNS 10_1101-2021_01_07_425737 20 5 localizing localize VBG 10_1101-2021_01_07_425737 20 6 to to IN 10_1101-2021_01_07_425737 20 7 the the DT 10_1101-2021_01_07_425737 20 8 outer outer JJ 10_1101-2021_01_07_425737 20 9 membrane membrane NN 10_1101-2021_01_07_425737 20 10 of of IN 10_1101-2021_01_07_425737 20 11 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 20 12 include include VBP 10_1101-2021_01_07_425737 20 13 small small JJ 10_1101-2021_01_07_425737 20 14 β β NN 10_1101-2021_01_07_425737 20 15 - - HYPH 10_1101-2021_01_07_425737 20 16 barrel barrel NN 10_1101-2021_01_07_425737 20 17 aquaporins aquaporin NNS 10_1101-2021_01_07_425737 20 18 , , , 10_1101-2021_01_07_425737 20 19 which which WDT 10_1101-2021_01_07_425737 20 20 allow allow VBP 10_1101-2021_01_07_425737 20 21 passive passive JJ 10_1101-2021_01_07_425737 20 22 diffusion diffusion NN 10_1101-2021_01_07_425737 20 23 of of IN 10_1101-2021_01_07_425737 20 24 small small JJ 10_1101-2021_01_07_425737 20 25 molecules molecule NNS 10_1101-2021_01_07_425737 20 26 , , , 10_1101-2021_01_07_425737 20 27 and and CC 10_1101-2021_01_07_425737 20 28 flagellum flagellum JJ 10_1101-2021_01_07_425737 20 29 basal basal NN 10_1101-2021_01_07_425737 20 30 body body NN 10_1101-2021_01_07_425737 20 31 components2,9,10 components2,9,10 JJ 10_1101-2021_01_07_425737 20 32 . . . 10_1101-2021_01_07_425737 21 1 Investigation investigation NN 10_1101-2021_01_07_425737 21 2 into into IN 10_1101-2021_01_07_425737 21 3 protein protein NN 10_1101-2021_01_07_425737 21 4 expression expression NN 10_1101-2021_01_07_425737 21 5 by by IN 10_1101-2021_01_07_425737 21 6 these these DT 10_1101-2021_01_07_425737 21 7 symbiotic symbiotic JJ 10_1101-2021_01_07_425737 21 8 partners partner NNS 10_1101-2021_01_07_425737 21 9 has have VBZ 10_1101-2021_01_07_425737 21 10 shown show VBN 10_1101-2021_01_07_425737 21 11 that that IN 10_1101-2021_01_07_425737 21 12 flagellum flagellum JJ 10_1101-2021_01_07_425737 21 13 basal basal NN 10_1101-2021_01_07_425737 21 14 body body NN 10_1101-2021_01_07_425737 21 15 components component NNS 10_1101-2021_01_07_425737 21 16 are be VBP 10_1101-2021_01_07_425737 21 17 highly highly RB 10_1101-2021_01_07_425737 21 18 expressed express VBN 10_1101-2021_01_07_425737 21 19 by by IN 10_1101-2021_01_07_425737 21 20 Buchnera11 Buchnera11 NNP 10_1101-2021_01_07_425737 21 21 . . . 10_1101-2021_01_07_425737 22 1 Indeed indeed RB 10_1101-2021_01_07_425737 22 2 , , , 10_1101-2021_01_07_425737 22 3 transmission transmission NN 10_1101-2021_01_07_425737 22 4 electron electron NN 10_1101-2021_01_07_425737 22 5 microscopy microscopy JJ 10_1101-2021_01_07_425737 22 6 images image NNS 10_1101-2021_01_07_425737 22 7 of of IN 10_1101-2021_01_07_425737 22 8 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 22 9 reveal reveal VBP 10_1101-2021_01_07_425737 22 10 flagellum flagellum NN 10_1101-2021_01_07_425737 22 11 basal basal NN 10_1101-2021_01_07_425737 22 12 bodies body NNS 10_1101-2021_01_07_425737 22 13 studded stud VBD 10_1101-2021_01_07_425737 22 14 all all RB 10_1101-2021_01_07_425737 22 15 over over IN 10_1101-2021_01_07_425737 22 16 the the DT 10_1101-2021_01_07_425737 22 17 bacterial bacterial JJ 10_1101-2021_01_07_425737 22 18 outer outer NN 10_1101-2021_01_07_425737 22 19 membrane12 membrane12 . 10_1101-2021_01_07_425737 22 20 . . . 10_1101-2021_01_07_425737 23 1 Despite despite IN 10_1101-2021_01_07_425737 23 2 its -PRON- PRP$ 10_1101-2021_01_07_425737 23 3 abundance abundance NN 10_1101-2021_01_07_425737 23 4 on on IN 10_1101-2021_01_07_425737 23 5 the the DT 10_1101-2021_01_07_425737 23 6 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 23 7 cell cell NN 10_1101-2021_01_07_425737 23 8 surface surface NN 10_1101-2021_01_07_425737 23 9 , , , 10_1101-2021_01_07_425737 23 10 the the DT 10_1101-2021_01_07_425737 23 11 role role NN 10_1101-2021_01_07_425737 23 12 of of IN 10_1101-2021_01_07_425737 23 13 this this DT 10_1101-2021_01_07_425737 23 14 protein protein NN 10_1101-2021_01_07_425737 23 15 complex complex NN 10_1101-2021_01_07_425737 23 16 for for IN 10_1101-2021_01_07_425737 23 17 maintaining maintain VBG 10_1101-2021_01_07_425737 23 18 the the DT 10_1101-2021_01_07_425737 23 19 aphid aphid NNP 10_1101-2021_01_07_425737 23 20 - - HYPH 10_1101-2021_01_07_425737 23 21 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 23 22 symbiosis symbiosis NN 10_1101-2021_01_07_425737 23 23 is be VBZ 10_1101-2021_01_07_425737 23 24 unknown13 unknown13 CD 10_1101-2021_01_07_425737 23 25 . . . 10_1101-2021_01_07_425737 24 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 24 2 of of IN 10_1101-2021_01_07_425737 24 3 the the DT 10_1101-2021_01_07_425737 24 4 pea pea NN 10_1101-2021_01_07_425737 24 5 aphid aphid NN 10_1101-2021_01_07_425737 24 6 ( ( -LRB- 10_1101-2021_01_07_425737 24 7 Acyrthosiphon Acyrthosiphon NNP 10_1101-2021_01_07_425737 24 8 pisum pisum NN 10_1101-2021_01_07_425737 24 9 ) ) -RRB- 10_1101-2021_01_07_425737 24 10 maintains maintain VBZ 10_1101-2021_01_07_425737 24 11 26 26 CD 10_1101-2021_01_07_425737 24 12 genes gene NNS 10_1101-2021_01_07_425737 24 13 coding code VBG 10_1101-2021_01_07_425737 24 14 for for IN 10_1101-2021_01_07_425737 24 15 flagellum flagellum JJ 10_1101-2021_01_07_425737 24 16 proteins protein NNS 10_1101-2021_01_07_425737 24 17 in in IN 10_1101-2021_01_07_425737 24 18 three three CD 10_1101-2021_01_07_425737 24 19 discrete discrete JJ 10_1101-2021_01_07_425737 24 20 clusters cluster NNS 10_1101-2021_01_07_425737 24 21 . . . 10_1101-2021_01_07_425737 25 1 The the DT 10_1101-2021_01_07_425737 25 2 maintained maintain VBN 10_1101-2021_01_07_425737 25 3 genes gene NNS 10_1101-2021_01_07_425737 25 4 code code NN 10_1101-2021_01_07_425737 25 5 for for IN 10_1101-2021_01_07_425737 25 6 the the DT 10_1101-2021_01_07_425737 25 7 structural structural JJ 10_1101-2021_01_07_425737 25 8 proteins protein NNS 10_1101-2021_01_07_425737 25 9 required require VBN 10_1101-2021_01_07_425737 25 10 for for IN 10_1101-2021_01_07_425737 25 11 formation formation NN 10_1101-2021_01_07_425737 25 12 of of IN 10_1101-2021_01_07_425737 25 13 a a DT 10_1101-2021_01_07_425737 25 14 flagellum flagellum JJ 10_1101-2021_01_07_425737 25 15 basal basal NN 10_1101-2021_01_07_425737 25 16 body body NN 10_1101-2021_01_07_425737 25 17 , , , 10_1101-2021_01_07_425737 25 18 a a DT 10_1101-2021_01_07_425737 25 19 partial partial JJ 10_1101-2021_01_07_425737 25 20 flagellar flagellar JJ 10_1101-2021_01_07_425737 25 21 hook hook NN 10_1101-2021_01_07_425737 25 22 , , , 10_1101-2021_01_07_425737 25 23 as as RB 10_1101-2021_01_07_425737 25 24 well well RB 10_1101-2021_01_07_425737 25 25 as as IN 10_1101-2021_01_07_425737 25 26 the the DT 10_1101-2021_01_07_425737 25 27 Type Type NNP 10_1101-2021_01_07_425737 25 28 III iii CD 10_1101-2021_01_07_425737 25 29 cytoplasmic cytoplasmic JJ 10_1101-2021_01_07_425737 25 30 export export NN 10_1101-2021_01_07_425737 25 31 proteins protein NNS 10_1101-2021_01_07_425737 25 32 . . . 10_1101-2021_01_07_425737 26 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 26 2 lineages lineage NNS 10_1101-2021_01_07_425737 26 3 vary vary VBP 10_1101-2021_01_07_425737 26 4 in in IN 10_1101-2021_01_07_425737 26 5 the the DT 10_1101-2021_01_07_425737 26 6 set set NN 10_1101-2021_01_07_425737 26 7 of of IN 10_1101-2021_01_07_425737 26 8 flagellum flagellum JJ 10_1101-2021_01_07_425737 26 9 genes gene NNS 10_1101-2021_01_07_425737 26 10 retained retain VBN 10_1101-2021_01_07_425737 26 11 ( ( -LRB- 10_1101-2021_01_07_425737 26 12 Supplementary Supplementary NNP 10_1101-2021_01_07_425737 26 13 Table Table NNP 10_1101-2021_01_07_425737 26 14 1 1 CD 10_1101-2021_01_07_425737 26 15 ) ) -RRB- 10_1101-2021_01_07_425737 26 16 , , , 10_1101-2021_01_07_425737 26 17 but but CC 10_1101-2021_01_07_425737 26 18 all all DT 10_1101-2021_01_07_425737 26 19 have have VBP 10_1101-2021_01_07_425737 26 20 lost lose VBN 10_1101-2021_01_07_425737 26 21 genes gene NNS 10_1101-2021_01_07_425737 26 22 encoding encode VBG 10_1101-2021_01_07_425737 26 23 the the DT 10_1101-2021_01_07_425737 26 24 flagellin flagellin NNP 10_1101-2021_01_07_425737 26 25 and and CC 10_1101-2021_01_07_425737 26 26 motor motor NNP 10_1101-2021_01_07_425737 26 27 proteins14 proteins14 VBP 10_1101-2021_01_07_425737 26 28 , , , 10_1101-2021_01_07_425737 26 29 indicating indicate VBG 10_1101-2021_01_07_425737 26 30 a a DT 10_1101-2021_01_07_425737 26 31 functional functional JJ 10_1101-2021_01_07_425737 26 32 shift shift NN 10_1101-2021_01_07_425737 26 33 away away RB 10_1101-2021_01_07_425737 26 34 from from IN 10_1101-2021_01_07_425737 26 35 cell cell NN 10_1101-2021_01_07_425737 26 36 motility motility NN 10_1101-2021_01_07_425737 26 37 . . . 10_1101-2021_01_07_425737 27 1 The the DT 10_1101-2021_01_07_425737 27 2 bacterial bacterial JJ 10_1101-2021_01_07_425737 27 3 flagellum flagellum NN 10_1101-2021_01_07_425737 27 4 structure structure NN 10_1101-2021_01_07_425737 27 5 is be VBZ 10_1101-2021_01_07_425737 27 6 an an DT 10_1101-2021_01_07_425737 27 7 evolutionary evolutionary JJ 10_1101-2021_01_07_425737 27 8 homologue homologue NN 10_1101-2021_01_07_425737 27 9 to to IN 10_1101-2021_01_07_425737 27 10 the the DT 10_1101-2021_01_07_425737 27 11 injectisome injectisome NN 10_1101-2021_01_07_425737 27 12 ( ( -LRB- 10_1101-2021_01_07_425737 27 13 Type type NN 10_1101-2021_01_07_425737 27 14 III III NNP 10_1101-2021_01_07_425737 27 15 secretion secretion NN 10_1101-2021_01_07_425737 27 16 system system NN 10_1101-2021_01_07_425737 27 17 , , , 10_1101-2021_01_07_425737 27 18 or or CC 10_1101-2021_01_07_425737 27 19 T3SS t3ss NN 10_1101-2021_01_07_425737 27 20 ) ) -RRB- 10_1101-2021_01_07_425737 27 21 , , , 10_1101-2021_01_07_425737 27 22 a a DT 10_1101-2021_01_07_425737 27 23 macromolecular macromolecular NN 10_1101-2021_01_07_425737 27 24 protein protein NN 10_1101-2021_01_07_425737 27 25 complex complex NN 10_1101-2021_01_07_425737 27 26 used use VBN 10_1101-2021_01_07_425737 27 27 to to IN 10_1101-2021_01_07_425737 27 28 ( ( -LRB- 10_1101-2021_01_07_425737 27 29 which which WDT 10_1101-2021_01_07_425737 27 30 was be VBD 10_1101-2021_01_07_425737 27 31 not not RB 10_1101-2021_01_07_425737 27 32 certified certify VBN 10_1101-2021_01_07_425737 27 33 by by IN 10_1101-2021_01_07_425737 27 34 peer peer NN 10_1101-2021_01_07_425737 27 35 review review NN 10_1101-2021_01_07_425737 27 36 ) ) -RRB- 10_1101-2021_01_07_425737 27 37 is be VBZ 10_1101-2021_01_07_425737 27 38 the the DT 10_1101-2021_01_07_425737 27 39 author author NN 10_1101-2021_01_07_425737 27 40 / / SYM 10_1101-2021_01_07_425737 27 41 funder funder NN 10_1101-2021_01_07_425737 27 42 . . . 10_1101-2021_01_07_425737 28 1 All all DT 10_1101-2021_01_07_425737 28 2 rights right NNS 10_1101-2021_01_07_425737 28 3 reserved reserve VBD 10_1101-2021_01_07_425737 28 4 . . . 10_1101-2021_01_07_425737 29 1 No no DT 10_1101-2021_01_07_425737 29 2 reuse reuse NN 10_1101-2021_01_07_425737 29 3 allowed allow VBN 10_1101-2021_01_07_425737 29 4 without without IN 10_1101-2021_01_07_425737 29 5 permission permission NN 10_1101-2021_01_07_425737 29 6 . . . 10_1101-2021_01_07_425737 30 1 The the DT 10_1101-2021_01_07_425737 30 2 copyright copyright NN 10_1101-2021_01_07_425737 30 3 holder holder NN 10_1101-2021_01_07_425737 30 4 for for IN 10_1101-2021_01_07_425737 30 5 this this DT 10_1101-2021_01_07_425737 30 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 30 7 version version NN 10_1101-2021_01_07_425737 30 8 posted post VBD 10_1101-2021_01_07_425737 30 9 January January NNP 10_1101-2021_01_07_425737 30 10 8 8 CD 10_1101-2021_01_07_425737 30 11 , , , 10_1101-2021_01_07_425737 30 12 2021 2021 CD 10_1101-2021_01_07_425737 30 13 . . . 10_1101-2021_01_07_425737 30 14 ; ; : 10_1101-2021_01_07_425737 30 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 30 16 : : : 10_1101-2021_01_07_425737 30 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 30 18 preprint preprint NN 10_1101-2021_01_07_425737 30 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 30 20 deliver deliver VB 10_1101-2021_01_07_425737 30 21 secrete secrete JJ 10_1101-2021_01_07_425737 30 22 effector effector NN 10_1101-2021_01_07_425737 30 23 proteins protein NNS 10_1101-2021_01_07_425737 30 24 , , , 10_1101-2021_01_07_425737 30 25 often often RB 10_1101-2021_01_07_425737 30 26 to to IN 10_1101-2021_01_07_425737 30 27 a a DT 10_1101-2021_01_07_425737 30 28 eukaryotic eukaryotic JJ 10_1101-2021_01_07_425737 30 29 host15,16,17 host15,16,17 NN 10_1101-2021_01_07_425737 30 30 . . . 10_1101-2021_01_07_425737 31 1 Flagellum Flagellum NNP 10_1101-2021_01_07_425737 31 2 assembly assembly NN 10_1101-2021_01_07_425737 31 3 occurs occur VBZ 10_1101-2021_01_07_425737 31 4 in in IN 10_1101-2021_01_07_425737 31 5 a a DT 10_1101-2021_01_07_425737 31 6 stepwise stepwise NN 10_1101-2021_01_07_425737 31 7 , , , 10_1101-2021_01_07_425737 31 8 sequential sequential JJ 10_1101-2021_01_07_425737 31 9 manner manner NN 10_1101-2021_01_07_425737 31 10 beginning begin VBG 10_1101-2021_01_07_425737 31 11 from from IN 10_1101-2021_01_07_425737 31 12 the the DT 10_1101-2021_01_07_425737 31 13 bacterial bacterial JJ 10_1101-2021_01_07_425737 31 14 cytoplasm cytoplasm NN 10_1101-2021_01_07_425737 31 15 , , , 10_1101-2021_01_07_425737 31 16 identical identical JJ 10_1101-2021_01_07_425737 31 17 to to IN 10_1101-2021_01_07_425737 31 18 the the DT 10_1101-2021_01_07_425737 31 19 T3SS18,19,20 T3SS18,19,20 NNP 10_1101-2021_01_07_425737 31 20 . . . 10_1101-2021_01_07_425737 32 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 32 2 maintains maintain VBZ 10_1101-2021_01_07_425737 32 3 genes gene NNS 10_1101-2021_01_07_425737 32 4 coding code VBG 10_1101-2021_01_07_425737 32 5 for for IN 10_1101-2021_01_07_425737 32 6 the the DT 10_1101-2021_01_07_425737 32 7 proteins protein NNS 10_1101-2021_01_07_425737 32 8 required require VBN 10_1101-2021_01_07_425737 32 9 for for IN 10_1101-2021_01_07_425737 32 10 a a DT 10_1101-2021_01_07_425737 32 11 functional functional JJ 10_1101-2021_01_07_425737 32 12 T3SS2,12 T3SS2,12 NNP 10_1101-2021_01_07_425737 32 13 , , , 10_1101-2021_01_07_425737 32 14 as as IN 10_1101-2021_01_07_425737 32 15 shown show VBN 10_1101-2021_01_07_425737 32 16 in in IN 10_1101-2021_01_07_425737 32 17 studies study NNS 10_1101-2021_01_07_425737 32 18 of of IN 10_1101-2021_01_07_425737 32 19 Yersinia21 Yersinia21 NNP 10_1101-2021_01_07_425737 32 20 , , , 10_1101-2021_01_07_425737 32 21 and and CC 10_1101-2021_01_07_425737 32 22 Salmonella22,23 Salmonella22,23 NNP 10_1101-2021_01_07_425737 32 23 . . . 10_1101-2021_01_07_425737 33 1 Gram gram NN 10_1101-2021_01_07_425737 33 2 - - HYPH 10_1101-2021_01_07_425737 33 3 negative negative JJ 10_1101-2021_01_07_425737 33 4 bacteria bacteria NNS 10_1101-2021_01_07_425737 33 5 have have VBP 10_1101-2021_01_07_425737 33 6 also also RB 10_1101-2021_01_07_425737 33 7 been be VBN 10_1101-2021_01_07_425737 33 8 shown show VBN 10_1101-2021_01_07_425737 33 9 to to IN 10_1101-2021_01_07_425737 33 10 export export NN 10_1101-2021_01_07_425737 33 11 proteins protein NNS 10_1101-2021_01_07_425737 33 12 through through IN 10_1101-2021_01_07_425737 33 13 a a DT 10_1101-2021_01_07_425737 33 14 flagellum flagellum JJ 10_1101-2021_01_07_425737 33 15 basal basal NNP 10_1101-2021_01_07_425737 33 16 body21,24,25 body21,24,25 NNP 10_1101-2021_01_07_425737 33 17 . . . 10_1101-2021_01_07_425737 34 1 The the DT 10_1101-2021_01_07_425737 34 2 bacterial bacterial JJ 10_1101-2021_01_07_425737 34 3 flagellum flagellum NN 10_1101-2021_01_07_425737 34 4 could could MD 10_1101-2021_01_07_425737 34 5 be be VB 10_1101-2021_01_07_425737 34 6 repurposed repurpose VBN 10_1101-2021_01_07_425737 34 7 to to TO 10_1101-2021_01_07_425737 34 8 serve serve VB 10_1101-2021_01_07_425737 34 9 a a DT 10_1101-2021_01_07_425737 34 10 novel novel JJ 10_1101-2021_01_07_425737 34 11 function function NN 10_1101-2021_01_07_425737 34 12 for for IN 10_1101-2021_01_07_425737 34 13 the the DT 10_1101-2021_01_07_425737 34 14 aphid aphid NNP 10_1101-2021_01_07_425737 34 15 - - HYPH 10_1101-2021_01_07_425737 34 16 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 34 17 symbiosis symbiosis NN 10_1101-2021_01_07_425737 34 18 . . . 10_1101-2021_01_07_425737 35 1 The the DT 10_1101-2021_01_07_425737 35 2 basal basal NN 10_1101-2021_01_07_425737 35 3 body body NN 10_1101-2021_01_07_425737 35 4 could could MD 10_1101-2021_01_07_425737 35 5 serve serve VB 10_1101-2021_01_07_425737 35 6 as as IN 10_1101-2021_01_07_425737 35 7 a a DT 10_1101-2021_01_07_425737 35 8 type type NN 10_1101-2021_01_07_425737 35 9 III iii CD 10_1101-2021_01_07_425737 35 10 protein protein NN 10_1101-2021_01_07_425737 35 11 exporter exporter NN 10_1101-2021_01_07_425737 35 12 to to TO 10_1101-2021_01_07_425737 35 13 secrete secrete VB 10_1101-2021_01_07_425737 35 14 proteins protein NNS 10_1101-2021_01_07_425737 35 15 to to TO 10_1101-2021_01_07_425737 35 16 signal signal VB 10_1101-2021_01_07_425737 35 17 to to IN 10_1101-2021_01_07_425737 35 18 the the DT 10_1101-2021_01_07_425737 35 19 aphid aphid NNP 10_1101-2021_01_07_425737 35 20 host host NN 10_1101-2021_01_07_425737 35 21 or or CC 10_1101-2021_01_07_425737 35 22 as as IN 10_1101-2021_01_07_425737 35 23 an an DT 10_1101-2021_01_07_425737 35 24 surface surface NN 10_1101-2021_01_07_425737 35 25 signal signal NN 10_1101-2021_01_07_425737 35 26 molecule molecule NN 10_1101-2021_01_07_425737 35 27 for for IN 10_1101-2021_01_07_425737 35 28 host host NN 10_1101-2021_01_07_425737 35 29 recognition recognition NN 10_1101-2021_01_07_425737 35 30 during during IN 10_1101-2021_01_07_425737 35 31 infection infection NN 10_1101-2021_01_07_425737 35 32 of of IN 10_1101-2021_01_07_425737 35 33 new new JJ 10_1101-2021_01_07_425737 35 34 aphid aphid NNP 10_1101-2021_01_07_425737 35 35 embryos embryos NNP 10_1101-2021_01_07_425737 35 36 . . . 10_1101-2021_01_07_425737 36 1 Here here RB 10_1101-2021_01_07_425737 36 2 , , , 10_1101-2021_01_07_425737 36 3 we -PRON- PRP 10_1101-2021_01_07_425737 36 4 present present VBP 10_1101-2021_01_07_425737 36 5 a a DT 10_1101-2021_01_07_425737 36 6 procedure procedure NN 10_1101-2021_01_07_425737 36 7 for for IN 10_1101-2021_01_07_425737 36 8 isolation isolation NN 10_1101-2021_01_07_425737 36 9 of of IN 10_1101-2021_01_07_425737 36 10 flagellum flagellum JJ 10_1101-2021_01_07_425737 36 11 basal basal NN 10_1101-2021_01_07_425737 36 12 body body NN 10_1101-2021_01_07_425737 36 13 complexes complex NNS 10_1101-2021_01_07_425737 36 14 adapted adapt VBN 10_1101-2021_01_07_425737 36 15 for for IN 10_1101-2021_01_07_425737 36 16 an an DT 10_1101-2021_01_07_425737 36 17 endosymbiont26 endosymbiont26 NNP 10_1101-2021_01_07_425737 36 18 , , , 10_1101-2021_01_07_425737 36 19 allowing allow VBG 10_1101-2021_01_07_425737 36 20 for for IN 10_1101-2021_01_07_425737 36 21 removal removal NN 10_1101-2021_01_07_425737 36 22 of of IN 10_1101-2021_01_07_425737 36 23 these these DT 10_1101-2021_01_07_425737 36 24 structures structure NNS 10_1101-2021_01_07_425737 36 25 directly directly RB 10_1101-2021_01_07_425737 36 26 from from IN 10_1101-2021_01_07_425737 36 27 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 36 28 and and CC 10_1101-2021_01_07_425737 36 29 enrichment enrichment NN 10_1101-2021_01_07_425737 36 30 of of IN 10_1101-2021_01_07_425737 36 31 flagellum flagellum JJ 10_1101-2021_01_07_425737 36 32 basal basal NN 10_1101-2021_01_07_425737 36 33 body body NN 10_1101-2021_01_07_425737 36 34 complexes complex NNS 10_1101-2021_01_07_425737 36 35 after after IN 10_1101-2021_01_07_425737 36 36 isolation isolation NN 10_1101-2021_01_07_425737 36 37 . . . 10_1101-2021_01_07_425737 37 1 This this DT 10_1101-2021_01_07_425737 37 2 procedure procedure NN 10_1101-2021_01_07_425737 37 3 will will MD 10_1101-2021_01_07_425737 37 4 enable enable VB 10_1101-2021_01_07_425737 37 5 further further JJ 10_1101-2021_01_07_425737 37 6 characterization characterization NN 10_1101-2021_01_07_425737 37 7 of of IN 10_1101-2021_01_07_425737 37 8 the the DT 10_1101-2021_01_07_425737 37 9 basal basal NN 10_1101-2021_01_07_425737 37 10 bodies body NNS 10_1101-2021_01_07_425737 37 11 and and CC 10_1101-2021_01_07_425737 37 12 their -PRON- PRP$ 10_1101-2021_01_07_425737 37 13 modifications modification NNS 10_1101-2021_01_07_425737 37 14 for for IN 10_1101-2021_01_07_425737 37 15 a a DT 10_1101-2021_01_07_425737 37 16 role role NN 10_1101-2021_01_07_425737 37 17 in in IN 10_1101-2021_01_07_425737 37 18 symbiosis symbiosis NN 10_1101-2021_01_07_425737 37 19 . . . 10_1101-2021_01_07_425737 38 1 Results result NNS 10_1101-2021_01_07_425737 38 2 Isolation isolation NN 10_1101-2021_01_07_425737 38 3 of of IN 10_1101-2021_01_07_425737 38 4 hook hook NN 10_1101-2021_01_07_425737 38 5 basal basal NN 10_1101-2021_01_07_425737 38 6 bodies body NNS 10_1101-2021_01_07_425737 38 7 from from IN 10_1101-2021_01_07_425737 38 8 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 38 9 Purification Purification NNP 10_1101-2021_01_07_425737 38 10 of of IN 10_1101-2021_01_07_425737 38 11 the the DT 10_1101-2021_01_07_425737 38 12 complex complex NN 10_1101-2021_01_07_425737 38 13 was be VBD 10_1101-2021_01_07_425737 38 14 initially initially RB 10_1101-2021_01_07_425737 38 15 assessed assess VBN 10_1101-2021_01_07_425737 38 16 at at IN 10_1101-2021_01_07_425737 38 17 multiple multiple JJ 10_1101-2021_01_07_425737 38 18 timepoints timepoint NNS 10_1101-2021_01_07_425737 38 19 along along IN 10_1101-2021_01_07_425737 38 20 the the DT 10_1101-2021_01_07_425737 38 21 procedure procedure NN 10_1101-2021_01_07_425737 38 22 . . . 10_1101-2021_01_07_425737 39 1 Samples sample NNS 10_1101-2021_01_07_425737 39 2 were be VBD 10_1101-2021_01_07_425737 39 3 taken take VBN 10_1101-2021_01_07_425737 39 4 of of IN 10_1101-2021_01_07_425737 39 5 initial initial JJ 10_1101-2021_01_07_425737 39 6 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 39 7 cell cell NN 10_1101-2021_01_07_425737 39 8 lysate lysate NN 10_1101-2021_01_07_425737 39 9 , , , 10_1101-2021_01_07_425737 39 10 lysate lysate NN 10_1101-2021_01_07_425737 39 11 after after IN 10_1101-2021_01_07_425737 39 12 raising raise VBG 10_1101-2021_01_07_425737 39 13 the the DT 10_1101-2021_01_07_425737 39 14 pH pH NNP 10_1101-2021_01_07_425737 39 15 to to IN 10_1101-2021_01_07_425737 39 16 10 10 CD 10_1101-2021_01_07_425737 39 17 , , , 10_1101-2021_01_07_425737 39 18 protein protein NN 10_1101-2021_01_07_425737 39 19 suspension suspension NN 10_1101-2021_01_07_425737 39 20 after after IN 10_1101-2021_01_07_425737 39 21 the the DT 10_1101-2021_01_07_425737 39 22 first first JJ 10_1101-2021_01_07_425737 39 23 5000 5000 CD 10_1101-2021_01_07_425737 39 24 g g NN 10_1101-2021_01_07_425737 39 25 spin spin NN 10_1101-2021_01_07_425737 39 26 , , , 10_1101-2021_01_07_425737 39 27 the the DT 10_1101-2021_01_07_425737 39 28 third third JJ 10_1101-2021_01_07_425737 39 29 5000 5000 CD 10_1101-2021_01_07_425737 39 30 g g NNP 10_1101-2021_01_07_425737 39 31 spin spin NN 10_1101-2021_01_07_425737 39 32 , , , 10_1101-2021_01_07_425737 39 33 and and CC 10_1101-2021_01_07_425737 39 34 finally finally RB 10_1101-2021_01_07_425737 39 35 after after IN 10_1101-2021_01_07_425737 39 36 the the DT 10_1101-2021_01_07_425737 39 37 30,000 30,000 CD 10_1101-2021_01_07_425737 39 38 g g NN 10_1101-2021_01_07_425737 39 39 spin spin NN 10_1101-2021_01_07_425737 39 40 and and CC 10_1101-2021_01_07_425737 39 41 overnight overnight JJ 10_1101-2021_01_07_425737 39 42 incubation incubation NN 10_1101-2021_01_07_425737 39 43 in in IN 10_1101-2021_01_07_425737 39 44 TET TET NNP 10_1101-2021_01_07_425737 39 45 buffer buffer NN 10_1101-2021_01_07_425737 39 46 . . . 10_1101-2021_01_07_425737 40 1 SDS SDS NNP 10_1101-2021_01_07_425737 40 2 - - HYPH 10_1101-2021_01_07_425737 40 3 PAGE PAGE NNP 10_1101-2021_01_07_425737 40 4 showed show VBD 10_1101-2021_01_07_425737 40 5 sixteen sixteen CD 10_1101-2021_01_07_425737 40 6 bands band NNS 10_1101-2021_01_07_425737 40 7 were be VBD 10_1101-2021_01_07_425737 40 8 present present JJ 10_1101-2021_01_07_425737 40 9 after after IN 10_1101-2021_01_07_425737 40 10 the the DT 10_1101-2021_01_07_425737 40 11 staining stain VBG 10_1101-2021_01_07_425737 40 12 procedure procedure NN 10_1101-2021_01_07_425737 40 13 and and CC 10_1101-2021_01_07_425737 40 14 their -PRON- PRP$ 10_1101-2021_01_07_425737 40 15 sizes size NNS 10_1101-2021_01_07_425737 40 16 corresponded correspond VBD 10_1101-2021_01_07_425737 40 17 to to IN 10_1101-2021_01_07_425737 40 18 those those DT 10_1101-2021_01_07_425737 40 19 of of IN 10_1101-2021_01_07_425737 40 20 constituent constituent NN 10_1101-2021_01_07_425737 40 21 proteins protein NNS 10_1101-2021_01_07_425737 40 22 of of IN 10_1101-2021_01_07_425737 40 23 the the DT 10_1101-2021_01_07_425737 40 24 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 40 25 flagellum flagellum NN 10_1101-2021_01_07_425737 40 26 basal basal NN 10_1101-2021_01_07_425737 40 27 body body NN 10_1101-2021_01_07_425737 40 28 ( ( -LRB- 10_1101-2021_01_07_425737 40 29 Supplemental supplemental JJ 10_1101-2021_01_07_425737 40 30 figure figure NN 10_1101-2021_01_07_425737 40 31 1 1 CD 10_1101-2021_01_07_425737 40 32 ) ) -RRB- 10_1101-2021_01_07_425737 40 33 . . . 10_1101-2021_01_07_425737 41 1 Protein protein NN 10_1101-2021_01_07_425737 41 2 samples sample NNS 10_1101-2021_01_07_425737 41 3 were be VBD 10_1101-2021_01_07_425737 41 4 extracted extract VBN 10_1101-2021_01_07_425737 41 5 from from IN 10_1101-2021_01_07_425737 41 6 the the DT 10_1101-2021_01_07_425737 41 7 gel gel NN 10_1101-2021_01_07_425737 41 8 and and CC 10_1101-2021_01_07_425737 41 9 subjected subject VBN 10_1101-2021_01_07_425737 41 10 to to IN 10_1101-2021_01_07_425737 41 11 mass mass NNP 10_1101-2021_01_07_425737 41 12 spectrometry spectrometry NN 10_1101-2021_01_07_425737 41 13 analysis analysis NN 10_1101-2021_01_07_425737 41 14 . . . 10_1101-2021_01_07_425737 42 1 Mass mass NN 10_1101-2021_01_07_425737 42 2 spectrometry spectrometry NN 10_1101-2021_01_07_425737 42 3 analysis analysis NN 10_1101-2021_01_07_425737 42 4 of of IN 10_1101-2021_01_07_425737 42 5 isolated isolated JJ 10_1101-2021_01_07_425737 42 6 basal basal NN 10_1101-2021_01_07_425737 42 7 bodies body NNS 10_1101-2021_01_07_425737 42 8 Protein Protein NNP 10_1101-2021_01_07_425737 42 9 ID ID NNP 10_1101-2021_01_07_425737 42 10 LC LC NNP 10_1101-2021_01_07_425737 42 11 - - HYPH 10_1101-2021_01_07_425737 42 12 MS MS NNP 10_1101-2021_01_07_425737 42 13 / / SYM 10_1101-2021_01_07_425737 42 14 MS MS NNP 10_1101-2021_01_07_425737 42 15 spectral spectral NN 10_1101-2021_01_07_425737 42 16 counts count NNS 10_1101-2021_01_07_425737 42 17 were be VBD 10_1101-2021_01_07_425737 42 18 provided provide VBN 10_1101-2021_01_07_425737 42 19 by by IN 10_1101-2021_01_07_425737 42 20 the the DT 10_1101-2021_01_07_425737 42 21 University University NNP 10_1101-2021_01_07_425737 42 22 of of IN 10_1101-2021_01_07_425737 42 23 Texas Texas NNP 10_1101-2021_01_07_425737 42 24 at at IN 10_1101-2021_01_07_425737 42 25 Austin Austin NNP 10_1101-2021_01_07_425737 42 26 Proteomics Proteomics NNP 10_1101-2021_01_07_425737 42 27 Core Core NNP 10_1101-2021_01_07_425737 42 28 Facility Facility NNP 10_1101-2021_01_07_425737 42 29 . . . 10_1101-2021_01_07_425737 43 1 We -PRON- PRP 10_1101-2021_01_07_425737 43 2 compared compare VBD 10_1101-2021_01_07_425737 43 3 our -PRON- PRP$ 10_1101-2021_01_07_425737 43 4 samples sample NNS 10_1101-2021_01_07_425737 43 5 to to IN 10_1101-2021_01_07_425737 43 6 proteomic proteomic JJ 10_1101-2021_01_07_425737 43 7 datasets dataset NNS 10_1101-2021_01_07_425737 43 8 from from IN 10_1101-2021_01_07_425737 43 9 homogenized homogenized JJ 10_1101-2021_01_07_425737 43 10 whole whole JJ 10_1101-2021_01_07_425737 43 11 aphids aphid NNS 10_1101-2021_01_07_425737 43 12 , , , 10_1101-2021_01_07_425737 43 13 and and CC 10_1101-2021_01_07_425737 43 14 from from IN 10_1101-2021_01_07_425737 43 15 bacteriocytes bacteriocyte NNS 10_1101-2021_01_07_425737 43 16 purified purify VBN 10_1101-2021_01_07_425737 43 17 from from IN 10_1101-2021_01_07_425737 43 18 pea pea NNP 10_1101-2021_01_07_425737 43 19 aphids11 aphids11 NNP 10_1101-2021_01_07_425737 43 20 . . . 10_1101-2021_01_07_425737 44 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 44 2 flagellum flagellum NN 10_1101-2021_01_07_425737 44 3 proteins protein NNS 10_1101-2021_01_07_425737 44 4 ( ( -LRB- 10_1101-2021_01_07_425737 44 5 which which WDT 10_1101-2021_01_07_425737 44 6 was be VBD 10_1101-2021_01_07_425737 44 7 not not RB 10_1101-2021_01_07_425737 44 8 certified certify VBN 10_1101-2021_01_07_425737 44 9 by by IN 10_1101-2021_01_07_425737 44 10 peer peer NN 10_1101-2021_01_07_425737 44 11 review review NN 10_1101-2021_01_07_425737 44 12 ) ) -RRB- 10_1101-2021_01_07_425737 44 13 is be VBZ 10_1101-2021_01_07_425737 44 14 the the DT 10_1101-2021_01_07_425737 44 15 author author NN 10_1101-2021_01_07_425737 44 16 / / SYM 10_1101-2021_01_07_425737 44 17 funder funder NN 10_1101-2021_01_07_425737 44 18 . . . 10_1101-2021_01_07_425737 45 1 All all DT 10_1101-2021_01_07_425737 45 2 rights right NNS 10_1101-2021_01_07_425737 45 3 reserved reserve VBD 10_1101-2021_01_07_425737 45 4 . . . 10_1101-2021_01_07_425737 46 1 No no DT 10_1101-2021_01_07_425737 46 2 reuse reuse NN 10_1101-2021_01_07_425737 46 3 allowed allow VBN 10_1101-2021_01_07_425737 46 4 without without IN 10_1101-2021_01_07_425737 46 5 permission permission NN 10_1101-2021_01_07_425737 46 6 . . . 10_1101-2021_01_07_425737 47 1 The the DT 10_1101-2021_01_07_425737 47 2 copyright copyright NN 10_1101-2021_01_07_425737 47 3 holder holder NN 10_1101-2021_01_07_425737 47 4 for for IN 10_1101-2021_01_07_425737 47 5 this this DT 10_1101-2021_01_07_425737 47 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 47 7 version version NN 10_1101-2021_01_07_425737 47 8 posted post VBD 10_1101-2021_01_07_425737 47 9 January January NNP 10_1101-2021_01_07_425737 47 10 8 8 CD 10_1101-2021_01_07_425737 47 11 , , , 10_1101-2021_01_07_425737 47 12 2021 2021 CD 10_1101-2021_01_07_425737 47 13 . . . 10_1101-2021_01_07_425737 47 14 ; ; : 10_1101-2021_01_07_425737 47 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 47 16 : : : 10_1101-2021_01_07_425737 47 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 47 18 preprint preprint NN 10_1101-2021_01_07_425737 47 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 47 20 were be VBD 10_1101-2021_01_07_425737 47 21 highly highly RB 10_1101-2021_01_07_425737 47 22 enriched enrich VBN 10_1101-2021_01_07_425737 47 23 by by IN 10_1101-2021_01_07_425737 47 24 our -PRON- PRP$ 10_1101-2021_01_07_425737 47 25 isolation isolation NN 10_1101-2021_01_07_425737 47 26 procedure procedure NN 10_1101-2021_01_07_425737 47 27 , , , 10_1101-2021_01_07_425737 47 28 especially especially RB 10_1101-2021_01_07_425737 47 29 FliF FliF NNP 10_1101-2021_01_07_425737 47 30 , , , 10_1101-2021_01_07_425737 47 31 FlgI FlgI NNP 10_1101-2021_01_07_425737 47 32 , , , 10_1101-2021_01_07_425737 47 33 FlgE FlgE NNP 10_1101-2021_01_07_425737 47 34 , , , 10_1101-2021_01_07_425737 47 35 FlhA FlhA NNP 10_1101-2021_01_07_425737 47 36 , , , 10_1101-2021_01_07_425737 47 37 and and CC 10_1101-2021_01_07_425737 47 38 FlgF FlgF NNP 10_1101-2021_01_07_425737 47 39 ( ( -LRB- 10_1101-2021_01_07_425737 47 40 Figure Figure NNP 10_1101-2021_01_07_425737 47 41 1 1 CD 10_1101-2021_01_07_425737 47 42 . . . 10_1101-2021_01_07_425737 47 43 ) ) -RRB- 10_1101-2021_01_07_425737 47 44 . . . 10_1101-2021_01_07_425737 48 1 These these DT 10_1101-2021_01_07_425737 48 2 results result NNS 10_1101-2021_01_07_425737 48 3 indicate indicate VBP 10_1101-2021_01_07_425737 48 4 that that IN 10_1101-2021_01_07_425737 48 5 all all DT 10_1101-2021_01_07_425737 48 6 but but IN 10_1101-2021_01_07_425737 48 7 two two CD 10_1101-2021_01_07_425737 48 8 flagellum flagellum JJ 10_1101-2021_01_07_425737 48 9 proteins protein NNS 10_1101-2021_01_07_425737 48 10 present present JJ 10_1101-2021_01_07_425737 48 11 in in IN 10_1101-2021_01_07_425737 48 12 the the DT 10_1101-2021_01_07_425737 48 13 mass mass JJ 10_1101-2021_01_07_425737 48 14 spectrometry spectrometry NN 10_1101-2021_01_07_425737 48 15 samples sample NNS 10_1101-2021_01_07_425737 48 16 were be VBD 10_1101-2021_01_07_425737 48 17 enriched enrich VBN 10_1101-2021_01_07_425737 48 18 during during IN 10_1101-2021_01_07_425737 48 19 the the DT 10_1101-2021_01_07_425737 48 20 isolation isolation NN 10_1101-2021_01_07_425737 48 21 procedure procedure NN 10_1101-2021_01_07_425737 48 22 : : : 10_1101-2021_01_07_425737 48 23 structural structural JJ 10_1101-2021_01_07_425737 48 24 proteins protein NNS 10_1101-2021_01_07_425737 48 25 FilE FilE NNP 10_1101-2021_01_07_425737 48 26 , , , 10_1101-2021_01_07_425737 48 27 FliF FliF NNP 10_1101-2021_01_07_425737 48 28 , , , 10_1101-2021_01_07_425737 48 29 FlgI FlgI NNP 10_1101-2021_01_07_425737 48 30 , , , 10_1101-2021_01_07_425737 48 31 FlgE FlgE NNP 10_1101-2021_01_07_425737 48 32 , , , 10_1101-2021_01_07_425737 48 33 FlgF FlgF NNP 10_1101-2021_01_07_425737 48 34 , , , 10_1101-2021_01_07_425737 48 35 and and CC 10_1101-2021_01_07_425737 48 36 FlgH FlgH NNP 10_1101-2021_01_07_425737 48 37 were be VBD 10_1101-2021_01_07_425737 48 38 enriched enrich VBN 10_1101-2021_01_07_425737 48 39 threefold threefold JJ 10_1101-2021_01_07_425737 48 40 or or CC 10_1101-2021_01_07_425737 48 41 more more JJR 10_1101-2021_01_07_425737 48 42 from from IN 10_1101-2021_01_07_425737 48 43 the the DT 10_1101-2021_01_07_425737 48 44 start start NN 10_1101-2021_01_07_425737 48 45 to to IN 10_1101-2021_01_07_425737 48 46 the the DT 10_1101-2021_01_07_425737 48 47 finish finish NN 10_1101-2021_01_07_425737 48 48 of of IN 10_1101-2021_01_07_425737 48 49 the the DT 10_1101-2021_01_07_425737 48 50 procedure procedure NN 10_1101-2021_01_07_425737 48 51 . . . 10_1101-2021_01_07_425737 49 1 FlgB FlgB NNP 10_1101-2021_01_07_425737 49 2 , , , 10_1101-2021_01_07_425737 49 3 FlgC FlgC NNP 10_1101-2021_01_07_425737 49 4 , , , 10_1101-2021_01_07_425737 49 5 FlgG FlgG NNP 10_1101-2021_01_07_425737 49 6 , , , 10_1101-2021_01_07_425737 49 7 FliG FliG NNP 10_1101-2021_01_07_425737 49 8 , , , 10_1101-2021_01_07_425737 49 9 FliH FliH NNP 10_1101-2021_01_07_425737 49 10 , , , 10_1101-2021_01_07_425737 49 11 and and CC 10_1101-2021_01_07_425737 49 12 FliI FliI NNP 10_1101-2021_01_07_425737 49 13 were be VBD 10_1101-2021_01_07_425737 49 14 enriched enrich VBN 10_1101-2021_01_07_425737 49 15 , , , 10_1101-2021_01_07_425737 49 16 though though IN 10_1101-2021_01_07_425737 49 17 not not RB 10_1101-2021_01_07_425737 49 18 to to IN 10_1101-2021_01_07_425737 49 19 the the DT 10_1101-2021_01_07_425737 49 20 extent extent NN 10_1101-2021_01_07_425737 49 21 of of IN 10_1101-2021_01_07_425737 49 22 the the DT 10_1101-2021_01_07_425737 49 23 other other JJ 10_1101-2021_01_07_425737 49 24 structural structural JJ 10_1101-2021_01_07_425737 49 25 proteins protein NNS 10_1101-2021_01_07_425737 49 26 . . . 10_1101-2021_01_07_425737 50 1 Type type NN 10_1101-2021_01_07_425737 50 2 III iii CD 10_1101-2021_01_07_425737 50 3 secretion secretion NN 10_1101-2021_01_07_425737 50 4 proteins protein VBZ 10_1101-2021_01_07_425737 50 5 FlhA FlhA NNP 10_1101-2021_01_07_425737 50 6 and and CC 10_1101-2021_01_07_425737 50 7 FliP FliP NNP 10_1101-2021_01_07_425737 50 8 were be VBD 10_1101-2021_01_07_425737 50 9 shown show VBN 10_1101-2021_01_07_425737 50 10 to to TO 10_1101-2021_01_07_425737 50 11 be be VB 10_1101-2021_01_07_425737 50 12 enriched enrich VBN 10_1101-2021_01_07_425737 50 13 by by IN 10_1101-2021_01_07_425737 50 14 this this DT 10_1101-2021_01_07_425737 50 15 procedure procedure NN 10_1101-2021_01_07_425737 50 16 ( ( -LRB- 10_1101-2021_01_07_425737 50 17 Figure figure NN 10_1101-2021_01_07_425737 50 18 2 2 CD 10_1101-2021_01_07_425737 50 19 . . CD 10_1101-2021_01_07_425737 50 20 , , , 10_1101-2021_01_07_425737 50 21 Supplemental supplemental JJ 10_1101-2021_01_07_425737 50 22 figure figure NN 10_1101-2021_01_07_425737 50 23 2 2 CD 10_1101-2021_01_07_425737 50 24 . . . 10_1101-2021_01_07_425737 50 25 ) ) -RRB- 10_1101-2021_01_07_425737 50 26 . . . 10_1101-2021_01_07_425737 51 1 The the DT 10_1101-2021_01_07_425737 51 2 widespread widespread JJ 10_1101-2021_01_07_425737 51 3 enrichment enrichment NN 10_1101-2021_01_07_425737 51 4 of of IN 10_1101-2021_01_07_425737 51 5 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 51 6 flagellum flagellum NN 10_1101-2021_01_07_425737 51 7 proteins protein NNS 10_1101-2021_01_07_425737 51 8 indicates indicate VBZ 10_1101-2021_01_07_425737 51 9 that that IN 10_1101-2021_01_07_425737 51 10 our -PRON- PRP$ 10_1101-2021_01_07_425737 51 11 adapted adapt VBN 10_1101-2021_01_07_425737 51 12 procedure procedure NN 10_1101-2021_01_07_425737 51 13 for for IN 10_1101-2021_01_07_425737 51 14 isolating isolate VBG 10_1101-2021_01_07_425737 51 15 macromolecular macromolecular JJ 10_1101-2021_01_07_425737 51 16 protein protein NN 10_1101-2021_01_07_425737 51 17 complexes complex NNS 10_1101-2021_01_07_425737 51 18 from from IN 10_1101-2021_01_07_425737 51 19 the the DT 10_1101-2021_01_07_425737 51 20 membranes membrane NNS 10_1101-2021_01_07_425737 51 21 of of IN 10_1101-2021_01_07_425737 51 22 endosymbiotic endosymbiotic JJ 10_1101-2021_01_07_425737 51 23 bacteria bacteria NNS 10_1101-2021_01_07_425737 51 24 was be VBD 10_1101-2021_01_07_425737 51 25 successful successful JJ 10_1101-2021_01_07_425737 51 26 . . . 10_1101-2021_01_07_425737 52 1 Only only RB 10_1101-2021_01_07_425737 52 2 flagellum flagellum JJ 10_1101-2021_01_07_425737 52 3 proteins protein NNS 10_1101-2021_01_07_425737 52 4 FlgK FlgK NNP 10_1101-2021_01_07_425737 52 5 and and CC 10_1101-2021_01_07_425737 52 6 FliN FliN NNP 10_1101-2021_01_07_425737 52 7 were be VBD 10_1101-2021_01_07_425737 52 8 reduced reduce VBN 10_1101-2021_01_07_425737 52 9 by by IN 10_1101-2021_01_07_425737 52 10 the the DT 10_1101-2021_01_07_425737 52 11 isolation isolation NN 10_1101-2021_01_07_425737 52 12 procedure procedure NN 10_1101-2021_01_07_425737 52 13 , , , 10_1101-2021_01_07_425737 52 14 perhaps perhaps RB 10_1101-2021_01_07_425737 52 15 because because IN 10_1101-2021_01_07_425737 52 16 of of IN 10_1101-2021_01_07_425737 52 17 their -PRON- PRP$ 10_1101-2021_01_07_425737 52 18 localization localization NN 10_1101-2021_01_07_425737 52 19 to to IN 10_1101-2021_01_07_425737 52 20 the the DT 10_1101-2021_01_07_425737 52 21 periphery periphery NN 10_1101-2021_01_07_425737 52 22 of of IN 10_1101-2021_01_07_425737 52 23 the the DT 10_1101-2021_01_07_425737 52 24 flagellum flagellum NN 10_1101-2021_01_07_425737 52 25 . . . 10_1101-2021_01_07_425737 53 1 Basal Basal NNP 10_1101-2021_01_07_425737 53 2 bodies body NNS 10_1101-2021_01_07_425737 53 3 resemble resemble VBP 10_1101-2021_01_07_425737 53 4 top top JJ 10_1101-2021_01_07_425737 53 5 hats hat NNS 10_1101-2021_01_07_425737 53 6 via via IN 10_1101-2021_01_07_425737 53 7 electron electron NN 10_1101-2021_01_07_425737 53 8 microscopy microscopy JJ 10_1101-2021_01_07_425737 53 9 We -PRON- PRP 10_1101-2021_01_07_425737 53 10 analyzed analyze VBD 10_1101-2021_01_07_425737 53 11 the the DT 10_1101-2021_01_07_425737 53 12 isolated isolated JJ 10_1101-2021_01_07_425737 53 13 basal basal NN 10_1101-2021_01_07_425737 53 14 bodies body NNS 10_1101-2021_01_07_425737 53 15 by by IN 10_1101-2021_01_07_425737 53 16 negative negative JJ 10_1101-2021_01_07_425737 53 17 stain stain NNP 10_1101-2021_01_07_425737 53 18 electron electron NNP 10_1101-2021_01_07_425737 53 19 microscopy microscopy JJ 10_1101-2021_01_07_425737 53 20 . . . 10_1101-2021_01_07_425737 54 1 While while IN 10_1101-2021_01_07_425737 54 2 raw raw JJ 10_1101-2021_01_07_425737 54 3 micrographs micrograph NNS 10_1101-2021_01_07_425737 54 4 showed show VBD 10_1101-2021_01_07_425737 54 5 heterogenous heterogenous JJ 10_1101-2021_01_07_425737 54 6 particles particle NNS 10_1101-2021_01_07_425737 54 7 , , , 10_1101-2021_01_07_425737 54 8 likely likely RB 10_1101-2021_01_07_425737 54 9 due due IN 10_1101-2021_01_07_425737 54 10 to to IN 10_1101-2021_01_07_425737 54 11 disassembly disassembly RB 10_1101-2021_01_07_425737 54 12 of of IN 10_1101-2021_01_07_425737 54 13 the the DT 10_1101-2021_01_07_425737 54 14 complex complex JJ 10_1101-2021_01_07_425737 54 15 , , , 10_1101-2021_01_07_425737 54 16 detergent detergent NN 10_1101-2021_01_07_425737 54 17 micelles micelle NNS 10_1101-2021_01_07_425737 54 18 , , , 10_1101-2021_01_07_425737 54 19 and and CC 10_1101-2021_01_07_425737 54 20 contaminating contaminate VBG 10_1101-2021_01_07_425737 54 21 proteins protein NNS 10_1101-2021_01_07_425737 54 22 , , , 10_1101-2021_01_07_425737 54 23 there there EX 10_1101-2021_01_07_425737 54 24 were be VBD 10_1101-2021_01_07_425737 54 25 several several JJ 10_1101-2021_01_07_425737 54 26 particles particle NNS 10_1101-2021_01_07_425737 54 27 that that WDT 10_1101-2021_01_07_425737 54 28 appeared appear VBD 10_1101-2021_01_07_425737 54 29 regularly regularly RB 10_1101-2021_01_07_425737 54 30 . . . 10_1101-2021_01_07_425737 55 1 These these DT 10_1101-2021_01_07_425737 55 2 single single JJ 10_1101-2021_01_07_425737 55 3 particles particle NNS 10_1101-2021_01_07_425737 55 4 resembled resemble VBD 10_1101-2021_01_07_425737 55 5 a a DT 10_1101-2021_01_07_425737 55 6 top top JJ 10_1101-2021_01_07_425737 55 7 hat hat NN 10_1101-2021_01_07_425737 55 8 with with IN 10_1101-2021_01_07_425737 55 9 both both DT 10_1101-2021_01_07_425737 55 10 rod rod NN 10_1101-2021_01_07_425737 55 11 and and CC 10_1101-2021_01_07_425737 55 12 ring ring NN 10_1101-2021_01_07_425737 55 13 - - HYPH 10_1101-2021_01_07_425737 55 14 shaped shape VBN 10_1101-2021_01_07_425737 55 15 features feature NNS 10_1101-2021_01_07_425737 55 16 ( ( -LRB- 10_1101-2021_01_07_425737 55 17 Figure figure NN 10_1101-2021_01_07_425737 55 18 3 3 CD 10_1101-2021_01_07_425737 55 19 ) ) -RRB- 10_1101-2021_01_07_425737 55 20 , , , 10_1101-2021_01_07_425737 55 21 similar similar JJ 10_1101-2021_01_07_425737 55 22 in in IN 10_1101-2021_01_07_425737 55 23 size size NN 10_1101-2021_01_07_425737 55 24 and and CC 10_1101-2021_01_07_425737 55 25 shape shape NN 10_1101-2021_01_07_425737 55 26 to to IN 10_1101-2021_01_07_425737 55 27 those those DT 10_1101-2021_01_07_425737 55 28 observed observe VBN 10_1101-2021_01_07_425737 55 29 in in IN 10_1101-2021_01_07_425737 55 30 TEM tem JJ 10_1101-2021_01_07_425737 55 31 images image NNS 10_1101-2021_01_07_425737 55 32 of of IN 10_1101-2021_01_07_425737 55 33 whole whole JJ 10_1101-2021_01_07_425737 55 34 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 55 35 cells12 cells12 NNP 10_1101-2021_01_07_425737 55 36 . . . 10_1101-2021_01_07_425737 56 1 Discussion discussion NN 10_1101-2021_01_07_425737 56 2 Here here RB 10_1101-2021_01_07_425737 56 3 , , , 10_1101-2021_01_07_425737 56 4 we -PRON- PRP 10_1101-2021_01_07_425737 56 5 demonstrate demonstrate VBP 10_1101-2021_01_07_425737 56 6 a a DT 10_1101-2021_01_07_425737 56 7 procedure procedure NN 10_1101-2021_01_07_425737 56 8 for for IN 10_1101-2021_01_07_425737 56 9 isolating isolate VBG 10_1101-2021_01_07_425737 56 10 macromolecular macromolecular JJ 10_1101-2021_01_07_425737 56 11 protein protein NN 10_1101-2021_01_07_425737 56 12 complexes complex NNS 10_1101-2021_01_07_425737 56 13 from from IN 10_1101-2021_01_07_425737 56 14 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 56 15 aphidicola aphidicola NNP 10_1101-2021_01_07_425737 56 16 , , , 10_1101-2021_01_07_425737 56 17 an an DT 10_1101-2021_01_07_425737 56 18 obligate obligate JJ 10_1101-2021_01_07_425737 56 19 endosymbiotic endosymbiotic JJ 10_1101-2021_01_07_425737 56 20 bacterium bacterium NN 10_1101-2021_01_07_425737 56 21 that that WDT 10_1101-2021_01_07_425737 56 22 can can MD 10_1101-2021_01_07_425737 56 23 not not RB 10_1101-2021_01_07_425737 56 24 be be VB 10_1101-2021_01_07_425737 56 25 cultured cultured JJ 10_1101-2021_01_07_425737 56 26 or or CC 10_1101-2021_01_07_425737 56 27 genetically genetically RB 10_1101-2021_01_07_425737 56 28 manipulated manipulate VBN 10_1101-2021_01_07_425737 56 29 . . . 10_1101-2021_01_07_425737 57 1 Identifying identify VBG 10_1101-2021_01_07_425737 57 2 the the DT 10_1101-2021_01_07_425737 57 3 changes change NNS 10_1101-2021_01_07_425737 57 4 in in IN 10_1101-2021_01_07_425737 57 5 these these DT 10_1101-2021_01_07_425737 57 6 complexes complex NNS 10_1101-2021_01_07_425737 57 7 could could MD 10_1101-2021_01_07_425737 57 8 elucidate elucidate VB 10_1101-2021_01_07_425737 57 9 how how WRB 10_1101-2021_01_07_425737 57 10 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 57 11 ’s ’s POS 10_1101-2021_01_07_425737 57 12 adaptation adaptation NN 10_1101-2021_01_07_425737 57 13 over over IN 10_1101-2021_01_07_425737 57 14 millions million NNS 10_1101-2021_01_07_425737 57 15 of of IN 10_1101-2021_01_07_425737 57 16 years year NNS 10_1101-2021_01_07_425737 57 17 to to IN 10_1101-2021_01_07_425737 57 18 a a DT 10_1101-2021_01_07_425737 57 19 mutualistic mutualistic JJ 10_1101-2021_01_07_425737 57 20 lifestyle lifestyle NN 10_1101-2021_01_07_425737 57 21 has have VBZ 10_1101-2021_01_07_425737 57 22 affected affect VBN 10_1101-2021_01_07_425737 57 23 its -PRON- PRP$ 10_1101-2021_01_07_425737 57 24 proteome proteome NN 10_1101-2021_01_07_425737 57 25 . . . 10_1101-2021_01_07_425737 58 1 As as IN 10_1101-2021_01_07_425737 58 2 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 58 3 is be VBZ 10_1101-2021_01_07_425737 58 4 not not RB 10_1101-2021_01_07_425737 58 5 motile motile JJ 10_1101-2021_01_07_425737 58 6 and and CC 10_1101-2021_01_07_425737 58 7 is be VBZ 10_1101-2021_01_07_425737 58 8 confined confine VBN 10_1101-2021_01_07_425737 58 9 to to IN 10_1101-2021_01_07_425737 58 10 host host NN 10_1101-2021_01_07_425737 58 11 - - VBN 10_1101-2021_01_07_425737 58 12 derived derive VBN 10_1101-2021_01_07_425737 58 13 “ " `` 10_1101-2021_01_07_425737 58 14 symbiosomal symbiosomal JJ 10_1101-2021_01_07_425737 58 15 ” " '' 10_1101-2021_01_07_425737 58 16 vesicles vesicle NNS 10_1101-2021_01_07_425737 58 17 inside inside IN 10_1101-2021_01_07_425737 58 18 bacteriocytes28,29 bacteriocytes28,29 NNP 10_1101-2021_01_07_425737 58 19 , , , 10_1101-2021_01_07_425737 58 20 the the DT 10_1101-2021_01_07_425737 58 21 retention retention NN 10_1101-2021_01_07_425737 58 22 and and CC 10_1101-2021_01_07_425737 58 23 expression expression NN 10_1101-2021_01_07_425737 58 24 of of IN 10_1101-2021_01_07_425737 58 25 these these DT 10_1101-2021_01_07_425737 58 26 partial partial JJ 10_1101-2021_01_07_425737 58 27 flagella flagella NN 10_1101-2021_01_07_425737 58 28 indicates indicate VBZ 10_1101-2021_01_07_425737 58 29 that that IN 10_1101-2021_01_07_425737 58 30 they -PRON- PRP 10_1101-2021_01_07_425737 58 31 have have VBP 10_1101-2021_01_07_425737 58 32 become become VBN 10_1101-2021_01_07_425737 58 33 repurposed repurposed JJ 10_1101-2021_01_07_425737 58 34 . . . 10_1101-2021_01_07_425737 59 1 These these DT 10_1101-2021_01_07_425737 59 2 complexes complex NNS 10_1101-2021_01_07_425737 59 3 have have VBP 10_1101-2021_01_07_425737 59 4 previously previously RB 10_1101-2021_01_07_425737 59 5 been be VBN 10_1101-2021_01_07_425737 59 6 hypothesized hypothesize VBN 10_1101-2021_01_07_425737 59 7 to to TO 10_1101-2021_01_07_425737 59 8 be be VB 10_1101-2021_01_07_425737 59 9 acting act VBG 10_1101-2021_01_07_425737 59 10 as as IN 10_1101-2021_01_07_425737 59 11 type type NN 10_1101-2021_01_07_425737 59 12 ( ( -LRB- 10_1101-2021_01_07_425737 59 13 which which WDT 10_1101-2021_01_07_425737 59 14 was be VBD 10_1101-2021_01_07_425737 59 15 not not RB 10_1101-2021_01_07_425737 59 16 certified certify VBN 10_1101-2021_01_07_425737 59 17 by by IN 10_1101-2021_01_07_425737 59 18 peer peer NN 10_1101-2021_01_07_425737 59 19 review review NN 10_1101-2021_01_07_425737 59 20 ) ) -RRB- 10_1101-2021_01_07_425737 59 21 is be VBZ 10_1101-2021_01_07_425737 59 22 the the DT 10_1101-2021_01_07_425737 59 23 author author NN 10_1101-2021_01_07_425737 59 24 / / SYM 10_1101-2021_01_07_425737 59 25 funder funder NN 10_1101-2021_01_07_425737 59 26 . . . 10_1101-2021_01_07_425737 60 1 All all DT 10_1101-2021_01_07_425737 60 2 rights right NNS 10_1101-2021_01_07_425737 60 3 reserved reserve VBD 10_1101-2021_01_07_425737 60 4 . . . 10_1101-2021_01_07_425737 61 1 No no DT 10_1101-2021_01_07_425737 61 2 reuse reuse NN 10_1101-2021_01_07_425737 61 3 allowed allow VBN 10_1101-2021_01_07_425737 61 4 without without IN 10_1101-2021_01_07_425737 61 5 permission permission NN 10_1101-2021_01_07_425737 61 6 . . . 10_1101-2021_01_07_425737 62 1 The the DT 10_1101-2021_01_07_425737 62 2 copyright copyright NN 10_1101-2021_01_07_425737 62 3 holder holder NN 10_1101-2021_01_07_425737 62 4 for for IN 10_1101-2021_01_07_425737 62 5 this this DT 10_1101-2021_01_07_425737 62 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 62 7 version version NN 10_1101-2021_01_07_425737 62 8 posted post VBD 10_1101-2021_01_07_425737 62 9 January January NNP 10_1101-2021_01_07_425737 62 10 8 8 CD 10_1101-2021_01_07_425737 62 11 , , , 10_1101-2021_01_07_425737 62 12 2021 2021 CD 10_1101-2021_01_07_425737 62 13 . . . 10_1101-2021_01_07_425737 62 14 ; ; : 10_1101-2021_01_07_425737 62 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 62 16 : : : 10_1101-2021_01_07_425737 62 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 62 18 preprint preprint NN 10_1101-2021_01_07_425737 62 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 62 20 III iii CD 10_1101-2021_01_07_425737 62 21 secretion secretion NN 10_1101-2021_01_07_425737 62 22 systems system NNS 10_1101-2021_01_07_425737 62 23 for for IN 10_1101-2021_01_07_425737 62 24 provisioning provision VBG 10_1101-2021_01_07_425737 62 25 peptides peptide NNS 10_1101-2021_01_07_425737 62 26 or or CC 10_1101-2021_01_07_425737 62 27 signal signal NN 10_1101-2021_01_07_425737 62 28 factors factor NNS 10_1101-2021_01_07_425737 62 29 to to IN 10_1101-2021_01_07_425737 62 30 the the DT 10_1101-2021_01_07_425737 62 31 aphid aphid NNP 10_1101-2021_01_07_425737 62 32 host13 host13 NNP 10_1101-2021_01_07_425737 62 33 . . . 10_1101-2021_01_07_425737 63 1 Indeed indeed RB 10_1101-2021_01_07_425737 63 2 , , , 10_1101-2021_01_07_425737 63 3 the the DT 10_1101-2021_01_07_425737 63 4 proteins protein NNS 10_1101-2021_01_07_425737 63 5 retained retain VBN 10_1101-2021_01_07_425737 63 6 in in IN 10_1101-2021_01_07_425737 63 7 the the DT 10_1101-2021_01_07_425737 63 8 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 63 9 flagellum flagellum NN 10_1101-2021_01_07_425737 63 10 constitute constitute VBP 10_1101-2021_01_07_425737 63 11 the the DT 10_1101-2021_01_07_425737 63 12 structural structural JJ 10_1101-2021_01_07_425737 63 13 proteins protein NNS 10_1101-2021_01_07_425737 63 14 and and CC 10_1101-2021_01_07_425737 63 15 machinery machinery NN 10_1101-2021_01_07_425737 63 16 required require VBN 10_1101-2021_01_07_425737 63 17 for for IN 10_1101-2021_01_07_425737 63 18 a a DT 10_1101-2021_01_07_425737 63 19 functional functional JJ 10_1101-2021_01_07_425737 63 20 type type NN 10_1101-2021_01_07_425737 63 21 III iii CD 10_1101-2021_01_07_425737 63 22 secretion secretion NN 10_1101-2021_01_07_425737 63 23 system21 system21 NN 10_1101-2021_01_07_425737 63 24 . . . 10_1101-2021_01_07_425737 64 1 Transcriptome transcriptome DT 10_1101-2021_01_07_425737 64 2 analyses analysis NNS 10_1101-2021_01_07_425737 64 3 of of IN 10_1101-2021_01_07_425737 64 4 pea pea JJ 10_1101-2021_01_07_425737 64 5 aphid aphid NNP 10_1101-2021_01_07_425737 64 6 lines line NNS 10_1101-2021_01_07_425737 64 7 with with IN 10_1101-2021_01_07_425737 64 8 different different JJ 10_1101-2021_01_07_425737 64 9 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 64 10 titers titer NNS 10_1101-2021_01_07_425737 64 11 reveal reveal VBP 10_1101-2021_01_07_425737 64 12 differences difference NNS 10_1101-2021_01_07_425737 64 13 in in IN 10_1101-2021_01_07_425737 64 14 expression expression NN 10_1101-2021_01_07_425737 64 15 of of IN 10_1101-2021_01_07_425737 64 16 flagellar flagellar NNP 10_1101-2021_01_07_425737 64 17 genes30 genes30 NNP 10_1101-2021_01_07_425737 64 18 . . . 10_1101-2021_01_07_425737 65 1 In in IN 10_1101-2021_01_07_425737 65 2 aphid aphid NNP 10_1101-2021_01_07_425737 65 3 lines line NNS 10_1101-2021_01_07_425737 65 4 that that WDT 10_1101-2021_01_07_425737 65 5 harbor harbor VBP 10_1101-2021_01_07_425737 65 6 relatively relatively RB 10_1101-2021_01_07_425737 65 7 low low JJ 10_1101-2021_01_07_425737 65 8 numbers number NNS 10_1101-2021_01_07_425737 65 9 of of IN 10_1101-2021_01_07_425737 65 10 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 65 11 , , , 10_1101-2021_01_07_425737 65 12 the the DT 10_1101-2021_01_07_425737 65 13 endosymbionts endosymbiont NNS 10_1101-2021_01_07_425737 65 14 have have VBP 10_1101-2021_01_07_425737 65 15 elevated elevate VBN 10_1101-2021_01_07_425737 65 16 relative relative JJ 10_1101-2021_01_07_425737 65 17 expression expression NN 10_1101-2021_01_07_425737 65 18 of of IN 10_1101-2021_01_07_425737 65 19 mRNA mRNA NNS 10_1101-2021_01_07_425737 65 20 associated associate VBN 10_1101-2021_01_07_425737 65 21 with with IN 10_1101-2021_01_07_425737 65 22 flagellar flagellar JJ 10_1101-2021_01_07_425737 65 23 secretion secretion NN 10_1101-2021_01_07_425737 65 24 genes gene NNS 10_1101-2021_01_07_425737 65 25 ( ( -LRB- 10_1101-2021_01_07_425737 65 26 fliP fliP NNS 10_1101-2021_01_07_425737 65 27 , , , 10_1101-2021_01_07_425737 65 28 fliQ fliq NN 10_1101-2021_01_07_425737 65 29 , , , 10_1101-2021_01_07_425737 65 30 and and CC 10_1101-2021_01_07_425737 65 31 fliR flir CD 10_1101-2021_01_07_425737 65 32 ) ) -RRB- 10_1101-2021_01_07_425737 65 33 , , , 10_1101-2021_01_07_425737 65 34 while while IN 10_1101-2021_01_07_425737 65 35 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 65 36 in in IN 10_1101-2021_01_07_425737 65 37 aphid aphid NNP 10_1101-2021_01_07_425737 65 38 lines line NNS 10_1101-2021_01_07_425737 65 39 with with IN 10_1101-2021_01_07_425737 65 40 high high JJ 10_1101-2021_01_07_425737 65 41 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 65 42 numbers number NNS 10_1101-2021_01_07_425737 65 43 had have VBD 10_1101-2021_01_07_425737 65 44 elevated elevate VBN 10_1101-2021_01_07_425737 65 45 expression expression NN 10_1101-2021_01_07_425737 65 46 of of IN 10_1101-2021_01_07_425737 65 47 genes gene NNS 10_1101-2021_01_07_425737 65 48 for for IN 10_1101-2021_01_07_425737 65 49 flagellum flagellum JJ 10_1101-2021_01_07_425737 65 50 structural structural JJ 10_1101-2021_01_07_425737 65 51 proteins30 proteins30 NNP 10_1101-2021_01_07_425737 65 52 Though though IN 10_1101-2021_01_07_425737 65 53 heavily heavily RB 10_1101-2021_01_07_425737 65 54 expressed express VBN 10_1101-2021_01_07_425737 65 55 in in IN 10_1101-2021_01_07_425737 65 56 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 65 57 of of IN 10_1101-2021_01_07_425737 65 58 pea pea NNP 10_1101-2021_01_07_425737 65 59 aphids aphid NNS 10_1101-2021_01_07_425737 65 60 , , , 10_1101-2021_01_07_425737 65 61 components component NNS 10_1101-2021_01_07_425737 65 62 of of IN 10_1101-2021_01_07_425737 65 63 the the DT 10_1101-2021_01_07_425737 65 64 flagellum flagellum JJ 10_1101-2021_01_07_425737 65 65 basal basal NN 10_1101-2021_01_07_425737 65 66 body body NN 10_1101-2021_01_07_425737 65 67 are be VBP 10_1101-2021_01_07_425737 65 68 not not RB 10_1101-2021_01_07_425737 65 69 maintained maintain VBN 10_1101-2021_01_07_425737 65 70 equally equally RB 10_1101-2021_01_07_425737 65 71 among among IN 10_1101-2021_01_07_425737 65 72 lineages lineage NNS 10_1101-2021_01_07_425737 65 73 of of IN 10_1101-2021_01_07_425737 65 74 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 65 75 of of IN 10_1101-2021_01_07_425737 65 76 different different JJ 10_1101-2021_01_07_425737 65 77 aphid aphid NNP 10_1101-2021_01_07_425737 65 78 species specie NNS 10_1101-2021_01_07_425737 65 79 based base VBN 10_1101-2021_01_07_425737 65 80 on on IN 10_1101-2021_01_07_425737 65 81 available available JJ 10_1101-2021_01_07_425737 65 82 genomic genomic JJ 10_1101-2021_01_07_425737 65 83 sequences14 sequences14 NN 10_1101-2021_01_07_425737 65 84 ( ( -LRB- 10_1101-2021_01_07_425737 65 85 Supplementary Supplementary NNP 10_1101-2021_01_07_425737 65 86 Table Table NNP 10_1101-2021_01_07_425737 65 87 1 1 CD 10_1101-2021_01_07_425737 65 88 ) ) -RRB- 10_1101-2021_01_07_425737 65 89 . . . 10_1101-2021_01_07_425737 66 1 Genes gene NNS 10_1101-2021_01_07_425737 66 2 coding code VBG 10_1101-2021_01_07_425737 66 3 for for IN 10_1101-2021_01_07_425737 66 4 proteins protein NNS 10_1101-2021_01_07_425737 66 5 associated associate VBN 10_1101-2021_01_07_425737 66 6 with with IN 10_1101-2021_01_07_425737 66 7 type type NN 10_1101-2021_01_07_425737 66 8 III III NNP 10_1101-2021_01_07_425737 66 9 secretion secretion NN 10_1101-2021_01_07_425737 66 10 activity activity NN 10_1101-2021_01_07_425737 66 11 ( ( -LRB- 10_1101-2021_01_07_425737 66 12 flhA flha CD 10_1101-2021_01_07_425737 66 13 , , , 10_1101-2021_01_07_425737 66 14 flhB flhb CD 10_1101-2021_01_07_425737 66 15 , , , 10_1101-2021_01_07_425737 66 16 fliP flip CD 10_1101-2021_01_07_425737 66 17 , , , 10_1101-2021_01_07_425737 66 18 fliQ fliq NN 10_1101-2021_01_07_425737 66 19 , , , 10_1101-2021_01_07_425737 66 20 and and CC 10_1101-2021_01_07_425737 66 21 fliR flir CD 10_1101-2021_01_07_425737 66 22 ) ) -RRB- 10_1101-2021_01_07_425737 66 23 and and CC 10_1101-2021_01_07_425737 66 24 basal basal NN 10_1101-2021_01_07_425737 66 25 body body NNP 10_1101-2021_01_07_425737 66 26 structural structural JJ 10_1101-2021_01_07_425737 66 27 proteins protein NNS 10_1101-2021_01_07_425737 66 28 ( ( -LRB- 10_1101-2021_01_07_425737 66 29 fliE flie CD 10_1101-2021_01_07_425737 66 30 , , , 10_1101-2021_01_07_425737 66 31 fliF flif CD 10_1101-2021_01_07_425737 66 32 , , , 10_1101-2021_01_07_425737 66 33 flgB flgb CD 10_1101-2021_01_07_425737 66 34 , , , 10_1101-2021_01_07_425737 66 35 flgC flgc CD 10_1101-2021_01_07_425737 66 36 , , , 10_1101-2021_01_07_425737 66 37 flgF flgf CD 10_1101-2021_01_07_425737 66 38 , , , 10_1101-2021_01_07_425737 66 39 flgG flgg NN 10_1101-2021_01_07_425737 66 40 , , , 10_1101-2021_01_07_425737 66 41 and and CC 10_1101-2021_01_07_425737 66 42 flgH flgh CD 10_1101-2021_01_07_425737 66 43 ) ) -RRB- 10_1101-2021_01_07_425737 66 44 are be VBP 10_1101-2021_01_07_425737 66 45 well well RB 10_1101-2021_01_07_425737 66 46 maintained maintain VBN 10_1101-2021_01_07_425737 66 47 across across IN 10_1101-2021_01_07_425737 66 48 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 66 49 lineages lineage NNS 10_1101-2021_01_07_425737 66 50 , , , 10_1101-2021_01_07_425737 66 51 but but CC 10_1101-2021_01_07_425737 66 52 genes gene NNS 10_1101-2021_01_07_425737 66 53 coding code VBG 10_1101-2021_01_07_425737 66 54 for for IN 10_1101-2021_01_07_425737 66 55 hook hook NN 10_1101-2021_01_07_425737 66 56 proteins protein NNS 10_1101-2021_01_07_425737 66 57 ( ( -LRB- 10_1101-2021_01_07_425737 66 58 flgD flgd CD 10_1101-2021_01_07_425737 66 59 , , , 10_1101-2021_01_07_425737 66 60 flgE flge CD 10_1101-2021_01_07_425737 66 61 , , , 10_1101-2021_01_07_425737 66 62 and and CC 10_1101-2021_01_07_425737 66 63 flgK flgk CD 10_1101-2021_01_07_425737 66 64 ) ) -RRB- 10_1101-2021_01_07_425737 66 65 and and CC 10_1101-2021_01_07_425737 66 66 the the DT 10_1101-2021_01_07_425737 66 67 flagellum flagellum NN 10_1101-2021_01_07_425737 66 68 - - HYPH 10_1101-2021_01_07_425737 66 69 specific specific JJ 10_1101-2021_01_07_425737 66 70 ATPase ATPase NNP 10_1101-2021_01_07_425737 66 71 ( ( -LRB- 10_1101-2021_01_07_425737 66 72 fliI fliI NNP 10_1101-2021_01_07_425737 66 73 ) ) -RRB- 10_1101-2021_01_07_425737 66 74 are be VBP 10_1101-2021_01_07_425737 66 75 frequently frequently RB 10_1101-2021_01_07_425737 66 76 shed shed VBN 10_1101-2021_01_07_425737 66 77 . . . 10_1101-2021_01_07_425737 67 1 A a DT 10_1101-2021_01_07_425737 67 2 more more RBR 10_1101-2021_01_07_425737 67 3 extreme extreme JJ 10_1101-2021_01_07_425737 67 4 example example NN 10_1101-2021_01_07_425737 67 5 is be VBZ 10_1101-2021_01_07_425737 67 6 the the DT 10_1101-2021_01_07_425737 67 7 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 67 8 strain strain NN 10_1101-2021_01_07_425737 67 9 harbored harbor VBN 10_1101-2021_01_07_425737 67 10 by by IN 10_1101-2021_01_07_425737 67 11 aphids aphid NNS 10_1101-2021_01_07_425737 67 12 of of IN 10_1101-2021_01_07_425737 67 13 genus genus NNP 10_1101-2021_01_07_425737 67 14 Stegophylla Stegophylla NNP 10_1101-2021_01_07_425737 67 15 : : : 10_1101-2021_01_07_425737 67 16 having have VBG 10_1101-2021_01_07_425737 67 17 the the DT 10_1101-2021_01_07_425737 67 18 smallest small JJS 10_1101-2021_01_07_425737 67 19 sequenced sequence VBN 10_1101-2021_01_07_425737 67 20 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 67 21 genome genome NN 10_1101-2021_01_07_425737 67 22 discovered discover VBN 10_1101-2021_01_07_425737 67 23 thus thus RB 10_1101-2021_01_07_425737 67 24 far far RB 10_1101-2021_01_07_425737 67 25 ( ( -LRB- 10_1101-2021_01_07_425737 67 26 412 412 CD 10_1101-2021_01_07_425737 67 27 kbps kbp NNS 10_1101-2021_01_07_425737 67 28 ) ) -RRB- 10_1101-2021_01_07_425737 67 29 , , , 10_1101-2021_01_07_425737 67 30 these these DT 10_1101-2021_01_07_425737 67 31 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 67 32 have have VBP 10_1101-2021_01_07_425737 67 33 completely completely RB 10_1101-2021_01_07_425737 67 34 lost lose VBN 10_1101-2021_01_07_425737 67 35 genes gene NNS 10_1101-2021_01_07_425737 67 36 associated associate VBN 10_1101-2021_01_07_425737 67 37 with with IN 10_1101-2021_01_07_425737 67 38 flagellum flagellum NN 10_1101-2021_01_07_425737 67 39 structure structure NN 10_1101-2021_01_07_425737 67 40 and and CC 10_1101-2021_01_07_425737 67 41 Type type NN 10_1101-2021_01_07_425737 67 42 III III NNP 10_1101-2021_01_07_425737 67 43 secretion secretion NN 10_1101-2021_01_07_425737 67 44 activity activity NN 10_1101-2021_01_07_425737 67 45 . . . 10_1101-2021_01_07_425737 68 1 In in IN 10_1101-2021_01_07_425737 68 2 all all DT 10_1101-2021_01_07_425737 68 3 but but IN 10_1101-2021_01_07_425737 68 4 the the DT 10_1101-2021_01_07_425737 68 5 most most RBS 10_1101-2021_01_07_425737 68 6 extreme extreme JJ 10_1101-2021_01_07_425737 68 7 examples example NNS 10_1101-2021_01_07_425737 68 8 , , , 10_1101-2021_01_07_425737 68 9 the the DT 10_1101-2021_01_07_425737 68 10 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 68 11 flagellum flagellum NN 10_1101-2021_01_07_425737 68 12 is be VBZ 10_1101-2021_01_07_425737 68 13 well well RB 10_1101-2021_01_07_425737 68 14 maintained maintain VBN 10_1101-2021_01_07_425737 68 15 , , , 10_1101-2021_01_07_425737 68 16 pointing point VBG 10_1101-2021_01_07_425737 68 17 to to IN 10_1101-2021_01_07_425737 68 18 a a DT 10_1101-2021_01_07_425737 68 19 continuing continue VBG 10_1101-2021_01_07_425737 68 20 role role NN 10_1101-2021_01_07_425737 68 21 for for IN 10_1101-2021_01_07_425737 68 22 this this DT 10_1101-2021_01_07_425737 68 23 complex complex NN 10_1101-2021_01_07_425737 68 24 for for IN 10_1101-2021_01_07_425737 68 25 this this DT 10_1101-2021_01_07_425737 68 26 ancient ancient JJ 10_1101-2021_01_07_425737 68 27 symbiosis symbiosis NN 10_1101-2021_01_07_425737 68 28 . . . 10_1101-2021_01_07_425737 69 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 69 2 ’s ’s POS 10_1101-2021_01_07_425737 69 3 tiny tiny JJ 10_1101-2021_01_07_425737 69 4 genome genome NN 10_1101-2021_01_07_425737 69 5 contains contain VBZ 10_1101-2021_01_07_425737 69 6 no no DT 10_1101-2021_01_07_425737 69 7 known known JJ 10_1101-2021_01_07_425737 69 8 pathogenicity pathogenicity NN 10_1101-2021_01_07_425737 69 9 proteins protein NNS 10_1101-2021_01_07_425737 69 10 or or CC 10_1101-2021_01_07_425737 69 11 proteins protein NNS 10_1101-2021_01_07_425737 69 12 previously previously RB 10_1101-2021_01_07_425737 69 13 associated associate VBN 10_1101-2021_01_07_425737 69 14 with with IN 10_1101-2021_01_07_425737 69 15 type type NN 10_1101-2021_01_07_425737 69 16 III III NNP 10_1101-2021_01_07_425737 69 17 export2,31 export2,31 NN 10_1101-2021_01_07_425737 69 18 . . . 10_1101-2021_01_07_425737 70 1 Potentially potentially RB 10_1101-2021_01_07_425737 70 2 , , , 10_1101-2021_01_07_425737 70 3 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 70 4 flagellum flagellum NN 10_1101-2021_01_07_425737 70 5 basal basal NN 10_1101-2021_01_07_425737 70 6 bodies body NNS 10_1101-2021_01_07_425737 70 7 may may MD 10_1101-2021_01_07_425737 70 8 instead instead RB 10_1101-2021_01_07_425737 70 9 serve serve VB 10_1101-2021_01_07_425737 70 10 as as IN 10_1101-2021_01_07_425737 70 11 surface surface NN 10_1101-2021_01_07_425737 70 12 signals signal NNS 10_1101-2021_01_07_425737 70 13 for for IN 10_1101-2021_01_07_425737 70 14 recognition recognition NN 10_1101-2021_01_07_425737 70 15 by by IN 10_1101-2021_01_07_425737 70 16 the the DT 10_1101-2021_01_07_425737 70 17 host host NN 10_1101-2021_01_07_425737 70 18 . . . 10_1101-2021_01_07_425737 71 1 Vertical vertical JJ 10_1101-2021_01_07_425737 71 2 transfer transfer NN 10_1101-2021_01_07_425737 71 3 of of IN 10_1101-2021_01_07_425737 71 4 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 71 5 from from IN 10_1101-2021_01_07_425737 71 6 mother mother NN 10_1101-2021_01_07_425737 71 7 to to IN 10_1101-2021_01_07_425737 71 8 daughter daughter NN 10_1101-2021_01_07_425737 71 9 aphids aphids NNP 10_1101-2021_01_07_425737 71 10 shows show VBZ 10_1101-2021_01_07_425737 71 11 naked naked JJ 10_1101-2021_01_07_425737 71 12 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 71 13 cells cell NNS 10_1101-2021_01_07_425737 71 14 being be VBG 10_1101-2021_01_07_425737 71 15 exocytosed exocytose VBN 10_1101-2021_01_07_425737 71 16 from from IN 10_1101-2021_01_07_425737 71 17 maternal maternal JJ 10_1101-2021_01_07_425737 71 18 bacteriocytes bacteriocyte NNS 10_1101-2021_01_07_425737 71 19 and and CC 10_1101-2021_01_07_425737 71 20 moving move VBG 10_1101-2021_01_07_425737 71 21 in in IN 10_1101-2021_01_07_425737 71 22 aphid aphid NNP 10_1101-2021_01_07_425737 71 23 haemolymph haemolymph NNP 10_1101-2021_01_07_425737 71 24 to to TO 10_1101-2021_01_07_425737 71 25 infect infect VB 10_1101-2021_01_07_425737 71 26 a a DT 10_1101-2021_01_07_425737 71 27 nearby nearby JJ 10_1101-2021_01_07_425737 71 28 specialized specialized JJ 10_1101-2021_01_07_425737 71 29 syncytial syncytial JJ 10_1101-2021_01_07_425737 71 30 cell cell NN 10_1101-2021_01_07_425737 71 31 of of IN 10_1101-2021_01_07_425737 71 32 stage stage NN 10_1101-2021_01_07_425737 71 33 7 7 CD 10_1101-2021_01_07_425737 71 34 embryos32 embryos32 CD 10_1101-2021_01_07_425737 71 35 . . . 10_1101-2021_01_07_425737 72 1 The the DT 10_1101-2021_01_07_425737 72 2 purpose purpose NN 10_1101-2021_01_07_425737 72 3 of of IN 10_1101-2021_01_07_425737 72 4 the the DT 10_1101-2021_01_07_425737 72 5 flagellum flagellum NN 10_1101-2021_01_07_425737 72 6 in in IN 10_1101-2021_01_07_425737 72 7 the the DT 10_1101-2021_01_07_425737 72 8 context context NN 10_1101-2021_01_07_425737 72 9 of of IN 10_1101-2021_01_07_425737 72 10 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 72 11 ’s ’s POS 10_1101-2021_01_07_425737 72 12 symbiotic symbiotic JJ 10_1101-2021_01_07_425737 72 13 lifestyle lifestyle NN 10_1101-2021_01_07_425737 72 14 remains remain VBZ 10_1101-2021_01_07_425737 72 15 unknown unknown JJ 10_1101-2021_01_07_425737 72 16 . . . 10_1101-2021_01_07_425737 73 1 ( ( -LRB- 10_1101-2021_01_07_425737 73 2 which which WDT 10_1101-2021_01_07_425737 73 3 was be VBD 10_1101-2021_01_07_425737 73 4 not not RB 10_1101-2021_01_07_425737 73 5 certified certify VBN 10_1101-2021_01_07_425737 73 6 by by IN 10_1101-2021_01_07_425737 73 7 peer peer NN 10_1101-2021_01_07_425737 73 8 review review NN 10_1101-2021_01_07_425737 73 9 ) ) -RRB- 10_1101-2021_01_07_425737 73 10 is be VBZ 10_1101-2021_01_07_425737 73 11 the the DT 10_1101-2021_01_07_425737 73 12 author author NN 10_1101-2021_01_07_425737 73 13 / / SYM 10_1101-2021_01_07_425737 73 14 funder funder NN 10_1101-2021_01_07_425737 73 15 . . . 10_1101-2021_01_07_425737 74 1 All all DT 10_1101-2021_01_07_425737 74 2 rights right NNS 10_1101-2021_01_07_425737 74 3 reserved reserve VBD 10_1101-2021_01_07_425737 74 4 . . . 10_1101-2021_01_07_425737 75 1 No no DT 10_1101-2021_01_07_425737 75 2 reuse reuse NN 10_1101-2021_01_07_425737 75 3 allowed allow VBN 10_1101-2021_01_07_425737 75 4 without without IN 10_1101-2021_01_07_425737 75 5 permission permission NN 10_1101-2021_01_07_425737 75 6 . . . 10_1101-2021_01_07_425737 76 1 The the DT 10_1101-2021_01_07_425737 76 2 copyright copyright NN 10_1101-2021_01_07_425737 76 3 holder holder NN 10_1101-2021_01_07_425737 76 4 for for IN 10_1101-2021_01_07_425737 76 5 this this DT 10_1101-2021_01_07_425737 76 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 76 7 version version NN 10_1101-2021_01_07_425737 76 8 posted post VBD 10_1101-2021_01_07_425737 76 9 January January NNP 10_1101-2021_01_07_425737 76 10 8 8 CD 10_1101-2021_01_07_425737 76 11 , , , 10_1101-2021_01_07_425737 76 12 2021 2021 CD 10_1101-2021_01_07_425737 76 13 . . . 10_1101-2021_01_07_425737 76 14 ; ; : 10_1101-2021_01_07_425737 76 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 76 16 : : : 10_1101-2021_01_07_425737 76 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 76 18 preprint preprint NN 10_1101-2021_01_07_425737 76 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 76 20 Further further JJ 10_1101-2021_01_07_425737 76 21 inquiry inquiry NN 10_1101-2021_01_07_425737 76 22 into into IN 10_1101-2021_01_07_425737 76 23 this this DT 10_1101-2021_01_07_425737 76 24 protein protein NN 10_1101-2021_01_07_425737 76 25 complex complex NN 10_1101-2021_01_07_425737 76 26 could could MD 10_1101-2021_01_07_425737 76 27 reveal reveal VB 10_1101-2021_01_07_425737 76 28 how how WRB 10_1101-2021_01_07_425737 76 29 the the DT 10_1101-2021_01_07_425737 76 30 repurposing repurposing NN 10_1101-2021_01_07_425737 76 31 of of IN 10_1101-2021_01_07_425737 76 32 a a DT 10_1101-2021_01_07_425737 76 33 motility motility NN 10_1101-2021_01_07_425737 76 34 organelle organelle NNP 10_1101-2021_01_07_425737 76 35 facilitates facilitate VBZ 10_1101-2021_01_07_425737 76 36 this this DT 10_1101-2021_01_07_425737 76 37 ancient ancient JJ 10_1101-2021_01_07_425737 76 38 and and CC 10_1101-2021_01_07_425737 76 39 obligate obligate NN 10_1101-2021_01_07_425737 76 40 symbiosis symbiosis NN 10_1101-2021_01_07_425737 76 41 . . . 10_1101-2021_01_07_425737 77 1 Methods method NNS 10_1101-2021_01_07_425737 77 2 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 77 3 extraction extraction NN 10_1101-2021_01_07_425737 77 4 from from IN 10_1101-2021_01_07_425737 77 5 aphids aphids NNP 10_1101-2021_01_07_425737 77 6 Pea Pea NNP 10_1101-2021_01_07_425737 77 7 aphids aphid NNS 10_1101-2021_01_07_425737 77 8 ( ( -LRB- 10_1101-2021_01_07_425737 77 9 Acrythosiphon Acrythosiphon NNP 10_1101-2021_01_07_425737 77 10 pisum pisum NN 10_1101-2021_01_07_425737 77 11 strain strain VBP 10_1101-2021_01_07_425737 77 12 LSR1 LSR1 NNP 10_1101-2021_01_07_425737 77 13 ) ) -RRB- 10_1101-2021_01_07_425737 77 14 were be VBD 10_1101-2021_01_07_425737 77 15 placed place VBN 10_1101-2021_01_07_425737 77 16 as as IN 10_1101-2021_01_07_425737 77 17 all all DT 10_1101-2021_01_07_425737 77 18 - - HYPH 10_1101-2021_01_07_425737 77 19 female female JJ 10_1101-2021_01_07_425737 77 20 clones clone NNS 10_1101-2021_01_07_425737 77 21 on on IN 10_1101-2021_01_07_425737 77 22 Fava Fava NNP 10_1101-2021_01_07_425737 77 23 bean bean NN 10_1101-2021_01_07_425737 77 24 ( ( -LRB- 10_1101-2021_01_07_425737 77 25 Vicia Vicia NNP 10_1101-2021_01_07_425737 77 26 faba faba CC 10_1101-2021_01_07_425737 77 27 ) ) -RRB- 10_1101-2021_01_07_425737 77 28 seedlings seedling NNS 10_1101-2021_01_07_425737 77 29 on on IN 10_1101-2021_01_07_425737 77 30 16h/8h 16h/8h CD 10_1101-2021_01_07_425737 77 31 light light JJ 10_1101-2021_01_07_425737 77 32 / / SYM 10_1101-2021_01_07_425737 77 33 dark dark JJ 10_1101-2021_01_07_425737 77 34 cycles cycle NNS 10_1101-2021_01_07_425737 77 35 at at IN 10_1101-2021_01_07_425737 77 36 20ºC. 20ºc. CD 10_1101-2021_01_07_425737 78 1 Once once RB 10_1101-2021_01_07_425737 78 2 reaching reach VBG 10_1101-2021_01_07_425737 78 3 adulthood adulthood NN 10_1101-2021_01_07_425737 78 4 , , , 10_1101-2021_01_07_425737 78 5 apterous apterous JJ 10_1101-2021_01_07_425737 78 6 adults adult NNS 10_1101-2021_01_07_425737 78 7 were be VBD 10_1101-2021_01_07_425737 78 8 raised raise VBN 10_1101-2021_01_07_425737 78 9 on on IN 10_1101-2021_01_07_425737 78 10 Fava Fava NNP 10_1101-2021_01_07_425737 78 11 bean bean NN 10_1101-2021_01_07_425737 78 12 plants plant NNS 10_1101-2021_01_07_425737 78 13 on on IN 10_1101-2021_01_07_425737 78 14 16h/8h 16h/8h CD 10_1101-2021_01_07_425737 78 15 light light JJ 10_1101-2021_01_07_425737 78 16 cycles cycle NNS 10_1101-2021_01_07_425737 78 17 and and CC 10_1101-2021_01_07_425737 78 18 allowed allow VBN 10_1101-2021_01_07_425737 78 19 to to TO 10_1101-2021_01_07_425737 78 20 reproduce reproduce VB 10_1101-2021_01_07_425737 78 21 . . . 10_1101-2021_01_07_425737 79 1 After after IN 10_1101-2021_01_07_425737 79 2 seven seven CD 10_1101-2021_01_07_425737 79 3 days day NNS 10_1101-2021_01_07_425737 79 4 , , , 10_1101-2021_01_07_425737 79 5 all all DT 10_1101-2021_01_07_425737 79 6 aphids aphid NNS 10_1101-2021_01_07_425737 79 7 ( ( -LRB- 10_1101-2021_01_07_425737 79 8 fourth fourth JJ 10_1101-2021_01_07_425737 79 9 - - HYPH 10_1101-2021_01_07_425737 79 10 instar instar NN 10_1101-2021_01_07_425737 79 11 larvae larvae NNP 10_1101-2021_01_07_425737 79 12 , , , 10_1101-2021_01_07_425737 79 13 typically typically RB 10_1101-2021_01_07_425737 79 14 amounting amount VBG 10_1101-2021_01_07_425737 79 15 to to IN 10_1101-2021_01_07_425737 79 16 5 5 CD 10_1101-2021_01_07_425737 79 17 g g NN 10_1101-2021_01_07_425737 79 18 ) ) -RRB- 10_1101-2021_01_07_425737 79 19 were be VBD 10_1101-2021_01_07_425737 79 20 removed remove VBN 10_1101-2021_01_07_425737 79 21 from from IN 10_1101-2021_01_07_425737 79 22 the the DT 10_1101-2021_01_07_425737 79 23 Fava Fava NNP 10_1101-2021_01_07_425737 79 24 bean bean NN 10_1101-2021_01_07_425737 79 25 plants plant NNS 10_1101-2021_01_07_425737 79 26 . . . 10_1101-2021_01_07_425737 80 1 Aphids Aphids NNP 10_1101-2021_01_07_425737 80 2 were be VBD 10_1101-2021_01_07_425737 80 3 weighed weigh VBN 10_1101-2021_01_07_425737 80 4 and and CC 10_1101-2021_01_07_425737 80 5 surface surface NN 10_1101-2021_01_07_425737 80 6 - - HYPH 10_1101-2021_01_07_425737 80 7 sterilized sterilize VBN 10_1101-2021_01_07_425737 80 8 in in IN 10_1101-2021_01_07_425737 80 9 0.5 0.5 CD 10_1101-2021_01_07_425737 80 10 % % NN 10_1101-2021_01_07_425737 80 11 bleach bleach NN 10_1101-2021_01_07_425737 80 12 solution solution NN 10_1101-2021_01_07_425737 80 13 , , , 10_1101-2021_01_07_425737 80 14 then then RB 10_1101-2021_01_07_425737 80 15 rinsed rinse VBD 10_1101-2021_01_07_425737 80 16 twice twice RB 10_1101-2021_01_07_425737 80 17 in in IN 10_1101-2021_01_07_425737 80 18 Ultrapure Ultrapure NNP 10_1101-2021_01_07_425737 80 19 water water NN 10_1101-2021_01_07_425737 80 20 ( ( -LRB- 10_1101-2021_01_07_425737 80 21 MilliporeSigma MilliporeSigma NNP 10_1101-2021_01_07_425737 80 22 ) ) -RRB- 10_1101-2021_01_07_425737 80 23 , , , 10_1101-2021_01_07_425737 80 24 each each DT 10_1101-2021_01_07_425737 80 25 30 30 CD 10_1101-2021_01_07_425737 80 26 seconds second NNS 10_1101-2021_01_07_425737 80 27 . . . 10_1101-2021_01_07_425737 81 1 Aphids aphid NNS 10_1101-2021_01_07_425737 81 2 were be VBD 10_1101-2021_01_07_425737 81 3 gently gently RB 10_1101-2021_01_07_425737 81 4 ground grind VBN 10_1101-2021_01_07_425737 81 5 in in IN 10_1101-2021_01_07_425737 81 6 a a DT 10_1101-2021_01_07_425737 81 7 mortar mortar NN 10_1101-2021_01_07_425737 81 8 and and CC 10_1101-2021_01_07_425737 81 9 pestle pestle NN 10_1101-2021_01_07_425737 81 10 in in IN 10_1101-2021_01_07_425737 81 11 40mL 40ml CD 10_1101-2021_01_07_425737 81 12 sterile sterile JJ 10_1101-2021_01_07_425737 81 13 Buffer Buffer NNP 10_1101-2021_01_07_425737 81 14 A a NN 10_1101-2021_01_07_425737 81 15 ( ( -LRB- 10_1101-2021_01_07_425737 81 16 25mM 25mm CD 10_1101-2021_01_07_425737 81 17 KCl KCl NNS 10_1101-2021_01_07_425737 81 18 ( ( -LRB- 10_1101-2021_01_07_425737 81 19 Sigma Sigma NNP 10_1101-2021_01_07_425737 81 20 - - HYPH 10_1101-2021_01_07_425737 81 21 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 81 22 ) ) -RRB- 10_1101-2021_01_07_425737 81 23 , , , 10_1101-2021_01_07_425737 81 24 35mM 35mm CD 10_1101-2021_01_07_425737 81 25 Tris Tris NNP 10_1101-2021_01_07_425737 81 26 base base NN 10_1101-2021_01_07_425737 81 27 ( ( -LRB- 10_1101-2021_01_07_425737 81 28 Sigma- Sigma- NNP 10_1101-2021_01_07_425737 81 29 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 81 30 ) ) -RRB- 10_1101-2021_01_07_425737 81 31 , , , 10_1101-2021_01_07_425737 81 32 10mM 10mm CD 10_1101-2021_01_07_425737 81 33 MgCl2 mgcl2 NN 10_1101-2021_01_07_425737 81 34 ( ( -LRB- 10_1101-2021_01_07_425737 81 35 Sigma Sigma NNP 10_1101-2021_01_07_425737 81 36 - - HYPH 10_1101-2021_01_07_425737 81 37 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 81 38 ) ) -RRB- 10_1101-2021_01_07_425737 81 39 , , , 10_1101-2021_01_07_425737 81 40 250mM 250mm CD 10_1101-2021_01_07_425737 81 41 anhydrous anhydrous JJ 10_1101-2021_01_07_425737 81 42 EDTA EDTA NNP 10_1101-2021_01_07_425737 81 43 ( ( -LRB- 10_1101-2021_01_07_425737 81 44 Sigma Sigma NNP 10_1101-2021_01_07_425737 81 45 - - HYPH 10_1101-2021_01_07_425737 81 46 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 81 47 ) ) -RRB- 10_1101-2021_01_07_425737 81 48 , , , 10_1101-2021_01_07_425737 81 49 and and CC 10_1101-2021_01_07_425737 81 50 500mM 500mm NN 10_1101-2021_01_07_425737 81 51 Sucrose Sucrose NNP 10_1101-2021_01_07_425737 81 52 ( ( -LRB- 10_1101-2021_01_07_425737 81 53 Sigma Sigma NNP 10_1101-2021_01_07_425737 81 54 - - HYPH 10_1101-2021_01_07_425737 81 55 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 81 56 ) ) -RRB- 10_1101-2021_01_07_425737 81 57 at at IN 10_1101-2021_01_07_425737 81 58 pH pH NNP 10_1101-2021_01_07_425737 81 59 7.5 7.5 CD 10_1101-2021_01_07_425737 81 60 ) ) -RRB- 10_1101-2021_01_07_425737 81 61 . . . 10_1101-2021_01_07_425737 82 1 Aphid Aphid NNP 10_1101-2021_01_07_425737 82 2 homogenate homogenate NN 10_1101-2021_01_07_425737 82 3 was be VBD 10_1101-2021_01_07_425737 82 4 vacuum vacuum NN 10_1101-2021_01_07_425737 82 5 filtered filter VBN 10_1101-2021_01_07_425737 82 6 to to IN 10_1101-2021_01_07_425737 82 7 100μm 100μm NNP 10_1101-2021_01_07_425737 82 8 , , , 10_1101-2021_01_07_425737 82 9 then then RB 10_1101-2021_01_07_425737 82 10 centrifuged centrifuge VBD 10_1101-2021_01_07_425737 82 11 at at IN 10_1101-2021_01_07_425737 82 12 1500 1500 CD 10_1101-2021_01_07_425737 82 13 g g NNP 10_1101-2021_01_07_425737 82 14 for for IN 10_1101-2021_01_07_425737 82 15 10 10 CD 10_1101-2021_01_07_425737 82 16 minutes minute NNS 10_1101-2021_01_07_425737 82 17 at at IN 10_1101-2021_01_07_425737 82 18 4C. 4c. CD 10_1101-2021_01_07_425737 83 1 Supernatant Supernatant NNP 10_1101-2021_01_07_425737 83 2 was be VBD 10_1101-2021_01_07_425737 83 3 discarded discard VBN 10_1101-2021_01_07_425737 83 4 , , , 10_1101-2021_01_07_425737 83 5 and and CC 10_1101-2021_01_07_425737 83 6 the the DT 10_1101-2021_01_07_425737 83 7 resulting result VBG 10_1101-2021_01_07_425737 83 8 pellet pellet NN 10_1101-2021_01_07_425737 83 9 was be VBD 10_1101-2021_01_07_425737 83 10 resuspended resuspend VBN 10_1101-2021_01_07_425737 83 11 in in IN 10_1101-2021_01_07_425737 83 12 20mL 20ml CD 10_1101-2021_01_07_425737 83 13 Buffer Buffer NNP 10_1101-2021_01_07_425737 83 14 A A NNP 10_1101-2021_01_07_425737 83 15 and and CC 10_1101-2021_01_07_425737 83 16 vacuum vacuum NN 10_1101-2021_01_07_425737 83 17 - - HYPH 10_1101-2021_01_07_425737 83 18 filtered filter VBN 10_1101-2021_01_07_425737 83 19 three three CD 10_1101-2021_01_07_425737 83 20 times time NNS 10_1101-2021_01_07_425737 83 21 from from IN 10_1101-2021_01_07_425737 83 22 20μm 20μm NN 10_1101-2021_01_07_425737 83 23 , , , 10_1101-2021_01_07_425737 83 24 to to IN 10_1101-2021_01_07_425737 83 25 10μm 10μm JJ 10_1101-2021_01_07_425737 83 26 , , , 10_1101-2021_01_07_425737 83 27 and and CC 10_1101-2021_01_07_425737 83 28 finally finally RB 10_1101-2021_01_07_425737 83 29 to to IN 10_1101-2021_01_07_425737 83 30 5μm 5μm NN 10_1101-2021_01_07_425737 83 31 . . . 10_1101-2021_01_07_425737 84 1 The the DT 10_1101-2021_01_07_425737 84 2 resulting result VBG 10_1101-2021_01_07_425737 84 3 filtrate filtrate NN 10_1101-2021_01_07_425737 84 4 was be VBD 10_1101-2021_01_07_425737 84 5 spun spin VBN 10_1101-2021_01_07_425737 84 6 at at IN 10_1101-2021_01_07_425737 84 7 1500 1500 CD 10_1101-2021_01_07_425737 84 8 g g NNP 10_1101-2021_01_07_425737 84 9 for for IN 10_1101-2021_01_07_425737 84 10 30 30 CD 10_1101-2021_01_07_425737 84 11 m m NN 10_1101-2021_01_07_425737 84 12 at at IN 10_1101-2021_01_07_425737 84 13 4C 4c JJ 10_1101-2021_01_07_425737 84 14 and and CC 10_1101-2021_01_07_425737 84 15 supernatant supernatant JJ 10_1101-2021_01_07_425737 84 16 discarded discard VBN 10_1101-2021_01_07_425737 84 17 . . . 10_1101-2021_01_07_425737 85 1 The the DT 10_1101-2021_01_07_425737 85 2 resulting result VBG 10_1101-2021_01_07_425737 85 3 pellet pellet NN 10_1101-2021_01_07_425737 85 4 was be VBD 10_1101-2021_01_07_425737 85 5 resuspended resuspend VBN 10_1101-2021_01_07_425737 85 6 in in IN 10_1101-2021_01_07_425737 85 7 10mL 10ml CD 10_1101-2021_01_07_425737 85 8 Sucrose Sucrose NNP 10_1101-2021_01_07_425737 85 9 solution solution NN 10_1101-2021_01_07_425737 85 10 ( ( -LRB- 10_1101-2021_01_07_425737 85 11 300mM 300mm NN 10_1101-2021_01_07_425737 85 12 sucrose sucrose NN 10_1101-2021_01_07_425737 85 13 ( ( -LRB- 10_1101-2021_01_07_425737 85 14 Sigma Sigma NNP 10_1101-2021_01_07_425737 85 15 - - HYPH 10_1101-2021_01_07_425737 85 16 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 85 17 ) ) -RRB- 10_1101-2021_01_07_425737 85 18 and and CC 10_1101-2021_01_07_425737 85 19 100mM 100mM NNP 10_1101-2021_01_07_425737 85 20 Tris Tris NNP 10_1101-2021_01_07_425737 85 21 base base NN 10_1101-2021_01_07_425737 85 22 ( ( -LRB- 10_1101-2021_01_07_425737 85 23 Sigma Sigma NNP 10_1101-2021_01_07_425737 85 24 - - HYPH 10_1101-2021_01_07_425737 85 25 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 85 26 ) ) -RRB- 10_1101-2021_01_07_425737 85 27 then then RB 10_1101-2021_01_07_425737 85 28 checked check VBD 10_1101-2021_01_07_425737 85 29 on on IN 10_1101-2021_01_07_425737 85 30 a a DT 10_1101-2021_01_07_425737 85 31 brightfield brightfield NN 10_1101-2021_01_07_425737 85 32 microscope microscope NN 10_1101-2021_01_07_425737 85 33 for for IN 10_1101-2021_01_07_425737 85 34 intact intact JJ 10_1101-2021_01_07_425737 85 35 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 85 36 cells cell NNS 10_1101-2021_01_07_425737 85 37 . . . 10_1101-2021_01_07_425737 86 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 86 2 cells cell NNS 10_1101-2021_01_07_425737 86 3 remain remain VBP 10_1101-2021_01_07_425737 86 4 alive alive JJ 10_1101-2021_01_07_425737 86 5 while while IN 10_1101-2021_01_07_425737 86 6 at at IN 10_1101-2021_01_07_425737 86 7 4C 4c NN 10_1101-2021_01_07_425737 86 8 for for IN 10_1101-2021_01_07_425737 86 9 a a DT 10_1101-2021_01_07_425737 86 10 maximum maximum NN 10_1101-2021_01_07_425737 86 11 of of IN 10_1101-2021_01_07_425737 86 12 24h 24h NNS 10_1101-2021_01_07_425737 86 13 . . . 10_1101-2021_01_07_425737 87 1 Isolation isolation NN 10_1101-2021_01_07_425737 87 2 of of IN 10_1101-2021_01_07_425737 87 3 flagellum flagellum JJ 10_1101-2021_01_07_425737 87 4 basal basal NN 10_1101-2021_01_07_425737 87 5 bodies body NNS 10_1101-2021_01_07_425737 87 6 from from IN 10_1101-2021_01_07_425737 87 7 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 87 8 cells cell NNS 10_1101-2021_01_07_425737 87 9 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 87 10 was be VBD 10_1101-2021_01_07_425737 87 11 incubated incubate VBN 10_1101-2021_01_07_425737 87 12 with with IN 10_1101-2021_01_07_425737 87 13 gentle gentle JJ 10_1101-2021_01_07_425737 87 14 spinning spinning NN 10_1101-2021_01_07_425737 87 15 on on IN 10_1101-2021_01_07_425737 87 16 ice ice NN 10_1101-2021_01_07_425737 87 17 with with IN 10_1101-2021_01_07_425737 87 18 egg egg NN 10_1101-2021_01_07_425737 87 19 white white JJ 10_1101-2021_01_07_425737 87 20 lysozyme lysozyme NNS 10_1101-2021_01_07_425737 87 21 ( ( -LRB- 10_1101-2021_01_07_425737 87 22 0.1mg 0.1mg NNP 10_1101-2021_01_07_425737 87 23 / / SYM 10_1101-2021_01_07_425737 87 24 mL mL NNP 10_1101-2021_01_07_425737 87 25 , , , 10_1101-2021_01_07_425737 87 26 Sigma Sigma NNP 10_1101-2021_01_07_425737 87 27 - - HYPH 10_1101-2021_01_07_425737 87 28 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 87 29 ) ) -RRB- 10_1101-2021_01_07_425737 87 30 for for IN 10_1101-2021_01_07_425737 87 31 30 30 CD 10_1101-2021_01_07_425737 87 32 m m NN 10_1101-2021_01_07_425737 87 33 . . . 10_1101-2021_01_07_425737 88 1 100mM 100mm LS 10_1101-2021_01_07_425737 88 2 Anhydrous anhydrous JJ 10_1101-2021_01_07_425737 88 3 EDTA edta NN 10_1101-2021_01_07_425737 88 4 solution solution NN 10_1101-2021_01_07_425737 88 5 , , , 10_1101-2021_01_07_425737 88 6 pH pH NNP 10_1101-2021_01_07_425737 88 7 7.5 7.5 CD 10_1101-2021_01_07_425737 88 8 ( ( -LRB- 10_1101-2021_01_07_425737 88 9 Sigma Sigma NNP 10_1101-2021_01_07_425737 88 10 - - HYPH 10_1101-2021_01_07_425737 88 11 Aldrich Aldrich NNP 10_1101-2021_01_07_425737 88 12 ) ) -RRB- 10_1101-2021_01_07_425737 88 13 was be VBD 10_1101-2021_01_07_425737 88 14 added add VBN 10_1101-2021_01_07_425737 88 15 to to IN 10_1101-2021_01_07_425737 88 16 final final JJ 10_1101-2021_01_07_425737 88 17 concentration concentration NN 10_1101-2021_01_07_425737 88 18 10mM. 10mm. CD 10_1101-2021_01_07_425737 89 1 The the DT 10_1101-2021_01_07_425737 89 2 pellet pellet NN 10_1101-2021_01_07_425737 89 3 was be VBD 10_1101-2021_01_07_425737 89 4 taken take VBN 10_1101-2021_01_07_425737 89 5 off off RP 10_1101-2021_01_07_425737 89 6 ice ice NN 10_1101-2021_01_07_425737 89 7 , , , 10_1101-2021_01_07_425737 89 8 and and CC 10_1101-2021_01_07_425737 89 9 gradually gradually RB 10_1101-2021_01_07_425737 89 10 raised raise VBN 10_1101-2021_01_07_425737 89 11 to to TO 10_1101-2021_01_07_425737 89 12 room room NN 10_1101-2021_01_07_425737 89 13 temperature temperature NN 10_1101-2021_01_07_425737 89 14 with with IN 10_1101-2021_01_07_425737 89 15 gentle gentle JJ 10_1101-2021_01_07_425737 89 16 spinning spinning NN 10_1101-2021_01_07_425737 89 17 for for IN 10_1101-2021_01_07_425737 89 18 30 30 CD 10_1101-2021_01_07_425737 89 19 m m NN 10_1101-2021_01_07_425737 89 20 . . . 10_1101-2021_01_07_425737 90 1 Triton Triton NNP 10_1101-2021_01_07_425737 90 2 X-100 X-100 NNP 10_1101-2021_01_07_425737 90 3 ( ( -LRB- 10_1101-2021_01_07_425737 90 4 Acros Acros NNP 10_1101-2021_01_07_425737 90 5 Organics Organics NNP 10_1101-2021_01_07_425737 90 6 ) ) -RRB- 10_1101-2021_01_07_425737 90 7 was be VBD 10_1101-2021_01_07_425737 90 8 added add VBN 10_1101-2021_01_07_425737 90 9 to to IN 10_1101-2021_01_07_425737 90 10 1 1 CD 10_1101-2021_01_07_425737 90 11 % % NN 10_1101-2021_01_07_425737 90 12 w w NN 10_1101-2021_01_07_425737 90 13 / / SYM 10_1101-2021_01_07_425737 90 14 v v NNP 10_1101-2021_01_07_425737 90 15 , , , 10_1101-2021_01_07_425737 90 16 along along IN 10_1101-2021_01_07_425737 90 17 with with IN 10_1101-2021_01_07_425737 90 18 1mg 1mg NN 10_1101-2021_01_07_425737 90 19 / / SYM 10_1101-2021_01_07_425737 90 20 mL mL NNP 10_1101-2021_01_07_425737 90 21 RNase rnase RB 10_1101-2021_01_07_425737 90 22 - - HYPH 10_1101-2021_01_07_425737 90 23 free free JJ 10_1101-2021_01_07_425737 90 24 DNase dnase IN 10_1101-2021_01_07_425737 90 25 I -PRON- PRP 10_1101-2021_01_07_425737 90 26 ( ( -LRB- 10_1101-2021_01_07_425737 90 27 Bovine Bovine NNP 10_1101-2021_01_07_425737 90 28 Pancreas Pancreas NNP 10_1101-2021_01_07_425737 90 29 , , , 10_1101-2021_01_07_425737 90 30 Sigma Sigma NNP 10_1101-2021_01_07_425737 90 31 - - HYPH 10_1101-2021_01_07_425737 90 32 Alrich Alrich NNP 10_1101-2021_01_07_425737 90 33 ) ) -RRB- 10_1101-2021_01_07_425737 90 34 and and CC 10_1101-2021_01_07_425737 90 35 allowed allow VBN 10_1101-2021_01_07_425737 90 36 to to TO 10_1101-2021_01_07_425737 90 37 stir stir VB 10_1101-2021_01_07_425737 90 38 for for IN 10_1101-2021_01_07_425737 90 39 1/2 1/2 CD 10_1101-2021_01_07_425737 90 40 hour hour NN 10_1101-2021_01_07_425737 90 41 . . . 10_1101-2021_01_07_425737 91 1 After after IN 10_1101-2021_01_07_425737 91 2 incubation incubation NN 10_1101-2021_01_07_425737 91 3 , , , 10_1101-2021_01_07_425737 91 4 cell cell NN 10_1101-2021_01_07_425737 91 5 lysate lysate NN 10_1101-2021_01_07_425737 91 6 was be VBD 10_1101-2021_01_07_425737 91 7 kept keep VBN 10_1101-2021_01_07_425737 91 8 at at IN 10_1101-2021_01_07_425737 91 9 4C 4c NN 10_1101-2021_01_07_425737 91 10 or or CC 10_1101-2021_01_07_425737 91 11 on on IN 10_1101-2021_01_07_425737 91 12 ice ice NN 10_1101-2021_01_07_425737 91 13 until until IN 10_1101-2021_01_07_425737 91 14 use use NN 10_1101-2021_01_07_425737 91 15 . . . 10_1101-2021_01_07_425737 92 1 The the DT 10_1101-2021_01_07_425737 92 2 lysate lysate NN 10_1101-2021_01_07_425737 92 3 was be VBD 10_1101-2021_01_07_425737 92 4 raised raise VBN 10_1101-2021_01_07_425737 92 5 to to IN 10_1101-2021_01_07_425737 92 6 pH pH NNP 10_1101-2021_01_07_425737 92 7 10 10 CD 10_1101-2021_01_07_425737 92 8 ( ( -LRB- 10_1101-2021_01_07_425737 92 9 which which WDT 10_1101-2021_01_07_425737 92 10 was be VBD 10_1101-2021_01_07_425737 92 11 not not RB 10_1101-2021_01_07_425737 92 12 certified certify VBN 10_1101-2021_01_07_425737 92 13 by by IN 10_1101-2021_01_07_425737 92 14 peer peer NN 10_1101-2021_01_07_425737 92 15 review review NN 10_1101-2021_01_07_425737 92 16 ) ) -RRB- 10_1101-2021_01_07_425737 92 17 is be VBZ 10_1101-2021_01_07_425737 92 18 the the DT 10_1101-2021_01_07_425737 92 19 author author NN 10_1101-2021_01_07_425737 92 20 / / SYM 10_1101-2021_01_07_425737 92 21 funder funder NN 10_1101-2021_01_07_425737 92 22 . . . 10_1101-2021_01_07_425737 93 1 All all DT 10_1101-2021_01_07_425737 93 2 rights right NNS 10_1101-2021_01_07_425737 93 3 reserved reserve VBD 10_1101-2021_01_07_425737 93 4 . . . 10_1101-2021_01_07_425737 94 1 No no DT 10_1101-2021_01_07_425737 94 2 reuse reuse NN 10_1101-2021_01_07_425737 94 3 allowed allow VBN 10_1101-2021_01_07_425737 94 4 without without IN 10_1101-2021_01_07_425737 94 5 permission permission NN 10_1101-2021_01_07_425737 94 6 . . . 10_1101-2021_01_07_425737 95 1 The the DT 10_1101-2021_01_07_425737 95 2 copyright copyright NN 10_1101-2021_01_07_425737 95 3 holder holder NN 10_1101-2021_01_07_425737 95 4 for for IN 10_1101-2021_01_07_425737 95 5 this this DT 10_1101-2021_01_07_425737 95 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 95 7 version version NN 10_1101-2021_01_07_425737 95 8 posted post VBD 10_1101-2021_01_07_425737 95 9 January January NNP 10_1101-2021_01_07_425737 95 10 8 8 CD 10_1101-2021_01_07_425737 95 11 , , , 10_1101-2021_01_07_425737 95 12 2021 2021 CD 10_1101-2021_01_07_425737 95 13 . . . 10_1101-2021_01_07_425737 95 14 ; ; : 10_1101-2021_01_07_425737 95 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 95 16 : : : 10_1101-2021_01_07_425737 95 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 95 18 preprint preprint NN 10_1101-2021_01_07_425737 95 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 95 20 using use VBG 10_1101-2021_01_07_425737 95 21 1N 1N NNP 10_1101-2021_01_07_425737 95 22 NaOH NaOH NNP 10_1101-2021_01_07_425737 95 23 ( ( -LRB- 10_1101-2021_01_07_425737 95 24 Macron Macron NNP 10_1101-2021_01_07_425737 95 25 Fine Fine NNP 10_1101-2021_01_07_425737 95 26 Chemicals Chemicals NNP 10_1101-2021_01_07_425737 95 27 ) ) -RRB- 10_1101-2021_01_07_425737 95 28 to to TO 10_1101-2021_01_07_425737 95 29 attempt attempt VB 10_1101-2021_01_07_425737 95 30 to to TO 10_1101-2021_01_07_425737 95 31 denature denature VB 10_1101-2021_01_07_425737 95 32 host host NN 10_1101-2021_01_07_425737 95 33 and and CC 10_1101-2021_01_07_425737 95 34 bacterial bacterial JJ 10_1101-2021_01_07_425737 95 35 cytoplasmic cytoplasmic JJ 10_1101-2021_01_07_425737 95 36 proteins protein NNS 10_1101-2021_01_07_425737 95 37 . . . 10_1101-2021_01_07_425737 96 1 The the DT 10_1101-2021_01_07_425737 96 2 solution solution NN 10_1101-2021_01_07_425737 96 3 was be VBD 10_1101-2021_01_07_425737 96 4 spun spin VBN 10_1101-2021_01_07_425737 96 5 at at IN 10_1101-2021_01_07_425737 96 6 5000 5000 CD 10_1101-2021_01_07_425737 96 7 g g NN 10_1101-2021_01_07_425737 96 8 for for IN 10_1101-2021_01_07_425737 96 9 10 10 CD 10_1101-2021_01_07_425737 96 10 m m NN 10_1101-2021_01_07_425737 96 11 at at IN 10_1101-2021_01_07_425737 96 12 4C 4C NNP 10_1101-2021_01_07_425737 96 13 three three CD 10_1101-2021_01_07_425737 96 14 times time NNS 10_1101-2021_01_07_425737 96 15 , , , 10_1101-2021_01_07_425737 96 16 each each DT 10_1101-2021_01_07_425737 96 17 time time NN 10_1101-2021_01_07_425737 96 18 decanting decant VBG 10_1101-2021_01_07_425737 96 19 the the DT 10_1101-2021_01_07_425737 96 20 supernatant supernatant JJ 10_1101-2021_01_07_425737 96 21 to to IN 10_1101-2021_01_07_425737 96 22 a a DT 10_1101-2021_01_07_425737 96 23 new new JJ 10_1101-2021_01_07_425737 96 24 tube tube NN 10_1101-2021_01_07_425737 96 25 . . . 10_1101-2021_01_07_425737 97 1 After after IN 10_1101-2021_01_07_425737 97 2 three three CD 10_1101-2021_01_07_425737 97 3 spins spin NNS 10_1101-2021_01_07_425737 97 4 , , , 10_1101-2021_01_07_425737 97 5 the the DT 10_1101-2021_01_07_425737 97 6 supernatant supernatant JJ 10_1101-2021_01_07_425737 97 7 was be VBD 10_1101-2021_01_07_425737 97 8 transferred transfer VBN 10_1101-2021_01_07_425737 97 9 to to IN 10_1101-2021_01_07_425737 97 10 a a DT 10_1101-2021_01_07_425737 97 11 Nalgene Nalgene NNP 10_1101-2021_01_07_425737 97 12 Oak Oak NNP 10_1101-2021_01_07_425737 97 13 Ridge Ridge NNP 10_1101-2021_01_07_425737 97 14 polyallomer polyallomer NN 10_1101-2021_01_07_425737 97 15 centrifuge centrifuge NN 10_1101-2021_01_07_425737 97 16 tube tube NN 10_1101-2021_01_07_425737 97 17 ( ( -LRB- 10_1101-2021_01_07_425737 97 18 Thermo Thermo NNP 10_1101-2021_01_07_425737 97 19 - - HYPH 10_1101-2021_01_07_425737 97 20 Fisher Fisher NNP 10_1101-2021_01_07_425737 97 21 ) ) -RRB- 10_1101-2021_01_07_425737 97 22 and and CC 10_1101-2021_01_07_425737 97 23 spun spin VBD 10_1101-2021_01_07_425737 97 24 at at IN 10_1101-2021_01_07_425737 97 25 30,000 30,000 CD 10_1101-2021_01_07_425737 97 26 g g NN 10_1101-2021_01_07_425737 97 27 for for IN 10_1101-2021_01_07_425737 97 28 1h 1h CD 10_1101-2021_01_07_425737 97 29 at at IN 10_1101-2021_01_07_425737 97 30 4C. 4c. CD 10_1101-2021_01_07_425737 98 1 Supernatant Supernatant NNP 10_1101-2021_01_07_425737 98 2 was be VBD 10_1101-2021_01_07_425737 98 3 gently gently RB 10_1101-2021_01_07_425737 98 4 decanted decant VBN 10_1101-2021_01_07_425737 98 5 and and CC 10_1101-2021_01_07_425737 98 6 pellet pellet JJ 10_1101-2021_01_07_425737 98 7 covered cover VBN 10_1101-2021_01_07_425737 98 8 with with IN 10_1101-2021_01_07_425737 98 9 TET TET NNP 10_1101-2021_01_07_425737 98 10 buffer buffer NN 10_1101-2021_01_07_425737 98 11 ( ( -LRB- 10_1101-2021_01_07_425737 98 12 10mM 10mm CD 10_1101-2021_01_07_425737 98 13 Tris Tris NNP 10_1101-2021_01_07_425737 98 14 - - HYPH 10_1101-2021_01_07_425737 98 15 HCl HCl NNP 10_1101-2021_01_07_425737 98 16 , , , 10_1101-2021_01_07_425737 98 17 5mM 5mm CD 10_1101-2021_01_07_425737 98 18 EDTA EDTA NNP 10_1101-2021_01_07_425737 98 19 , , , 10_1101-2021_01_07_425737 98 20 0.1 0.1 CD 10_1101-2021_01_07_425737 98 21 % % NN 10_1101-2021_01_07_425737 98 22 X- X- NNP 10_1101-2021_01_07_425737 98 23 100 100 CD 10_1101-2021_01_07_425737 98 24 , , , 10_1101-2021_01_07_425737 98 25 pH pH NNP 10_1101-2021_01_07_425737 98 26 8.0 8.0 CD 10_1101-2021_01_07_425737 98 27 ) ) -RRB- 10_1101-2021_01_07_425737 98 28 and and CC 10_1101-2021_01_07_425737 98 29 left leave VBD 10_1101-2021_01_07_425737 98 30 overnight overnight RB 10_1101-2021_01_07_425737 98 31 at at IN 10_1101-2021_01_07_425737 98 32 4C 4c NN 10_1101-2021_01_07_425737 98 33 to to TO 10_1101-2021_01_07_425737 98 34 soften soften VB 10_1101-2021_01_07_425737 98 35 and and CC 10_1101-2021_01_07_425737 98 36 dissolve dissolve VB 10_1101-2021_01_07_425737 98 37 . . . 10_1101-2021_01_07_425737 99 1 Submission submission NN 10_1101-2021_01_07_425737 99 2 of of IN 10_1101-2021_01_07_425737 99 3 protein protein NN 10_1101-2021_01_07_425737 99 4 for for IN 10_1101-2021_01_07_425737 99 5 mass mass NN 10_1101-2021_01_07_425737 99 6 spectrometry spectrometry NN 10_1101-2021_01_07_425737 99 7 Solubilized solubilized JJ 10_1101-2021_01_07_425737 99 8 protein protein NN 10_1101-2021_01_07_425737 99 9 concentration concentration NN 10_1101-2021_01_07_425737 99 10 was be VBD 10_1101-2021_01_07_425737 99 11 determined determine VBN 10_1101-2021_01_07_425737 99 12 using use VBG 10_1101-2021_01_07_425737 99 13 an an DT 10_1101-2021_01_07_425737 99 14 Eppendorf Eppendorf NNP 10_1101-2021_01_07_425737 99 15 Biophotometer Biophotometer NNP 10_1101-2021_01_07_425737 99 16 . . . 10_1101-2021_01_07_425737 100 1 1.5 1.5 CD 10_1101-2021_01_07_425737 100 2 mg mg NNP 10_1101-2021_01_07_425737 100 3 protein protein NN 10_1101-2021_01_07_425737 100 4 was be VBD 10_1101-2021_01_07_425737 100 5 run run VBN 10_1101-2021_01_07_425737 100 6 on on IN 10_1101-2021_01_07_425737 100 7 premade premade NN 10_1101-2021_01_07_425737 100 8 4 4 CD 10_1101-2021_01_07_425737 100 9 - - SYM 10_1101-2021_01_07_425737 100 10 12 12 CD 10_1101-2021_01_07_425737 100 11 % % NN 10_1101-2021_01_07_425737 100 12 Tris Tris NNP 10_1101-2021_01_07_425737 100 13 - - HYPH 10_1101-2021_01_07_425737 100 14 Glycine Glycine NNP 10_1101-2021_01_07_425737 100 15 SDS SDS NNP 10_1101-2021_01_07_425737 100 16 - - HYPH 10_1101-2021_01_07_425737 100 17 PAGE PAGE NNP 10_1101-2021_01_07_425737 100 18 gels gel NNS 10_1101-2021_01_07_425737 100 19 ( ( -LRB- 10_1101-2021_01_07_425737 100 20 Thermo Thermo NNP 10_1101-2021_01_07_425737 100 21 - - HYPH 10_1101-2021_01_07_425737 100 22 Fisher Fisher NNP 10_1101-2021_01_07_425737 100 23 ) ) -RRB- 10_1101-2021_01_07_425737 100 24 at at IN 10_1101-2021_01_07_425737 100 25 120V 120v CD 10_1101-2021_01_07_425737 100 26 for for IN 10_1101-2021_01_07_425737 100 27 10 10 CD 10_1101-2021_01_07_425737 100 28 m m NN 10_1101-2021_01_07_425737 100 29 . . . 10_1101-2021_01_07_425737 101 1 Gels gel NNS 10_1101-2021_01_07_425737 101 2 were be VBD 10_1101-2021_01_07_425737 101 3 stained stain VBN 10_1101-2021_01_07_425737 101 4 in in IN 10_1101-2021_01_07_425737 101 5 Coomassie Coomassie NNP 10_1101-2021_01_07_425737 101 6 Brilliant Brilliant NNP 10_1101-2021_01_07_425737 101 7 Blue Blue NNP 10_1101-2021_01_07_425737 101 8 ( ( -LRB- 10_1101-2021_01_07_425737 101 9 Bio Bio NNP 10_1101-2021_01_07_425737 101 10 - - HYPH 10_1101-2021_01_07_425737 101 11 Rad Rad NNP 10_1101-2021_01_07_425737 101 12 ) ) -RRB- 10_1101-2021_01_07_425737 101 13 for for IN 10_1101-2021_01_07_425737 101 14 30 30 CD 10_1101-2021_01_07_425737 101 15 m m CD 10_1101-2021_01_07_425737 101 16 , , , 10_1101-2021_01_07_425737 101 17 then then RB 10_1101-2021_01_07_425737 101 18 destained destain VBD 10_1101-2021_01_07_425737 101 19 in in IN 10_1101-2021_01_07_425737 101 20 20 20 CD 10_1101-2021_01_07_425737 101 21 % % NN 10_1101-2021_01_07_425737 101 22 Acetic acetic JJ 10_1101-2021_01_07_425737 101 23 acid acid NN 10_1101-2021_01_07_425737 101 24 ( ( -LRB- 10_1101-2021_01_07_425737 101 25 Thermo Thermo NNP 10_1101-2021_01_07_425737 101 26 - - HYPH 10_1101-2021_01_07_425737 101 27 Fisher Fisher NNP 10_1101-2021_01_07_425737 101 28 ) ) -RRB- 10_1101-2021_01_07_425737 101 29 for for IN 10_1101-2021_01_07_425737 101 30 30 30 CD 10_1101-2021_01_07_425737 101 31 m m NN 10_1101-2021_01_07_425737 101 32 . . . 10_1101-2021_01_07_425737 102 1 Gel Gel NNP 10_1101-2021_01_07_425737 102 2 bands band NNS 10_1101-2021_01_07_425737 102 3 corresponding correspond VBG 10_1101-2021_01_07_425737 102 4 to to IN 10_1101-2021_01_07_425737 102 5 the the DT 10_1101-2021_01_07_425737 102 6 step step NN 10_1101-2021_01_07_425737 102 7 in in IN 10_1101-2021_01_07_425737 102 8 the the DT 10_1101-2021_01_07_425737 102 9 procedure procedure NN 10_1101-2021_01_07_425737 102 10 sampled sample VBN 10_1101-2021_01_07_425737 102 11 ( ( -LRB- 10_1101-2021_01_07_425737 102 12 “ " `` 10_1101-2021_01_07_425737 102 13 Lysate Lysate NNP 10_1101-2021_01_07_425737 102 14 , , , 10_1101-2021_01_07_425737 102 15 ” " '' 10_1101-2021_01_07_425737 102 16 “ " `` 10_1101-2021_01_07_425737 102 17 pH pH NNP 10_1101-2021_01_07_425737 102 18 10 10 CD 10_1101-2021_01_07_425737 102 19 , , , 10_1101-2021_01_07_425737 102 20 ” " '' 10_1101-2021_01_07_425737 102 21 “ " `` 10_1101-2021_01_07_425737 102 22 Spin spin NN 10_1101-2021_01_07_425737 102 23 1 1 CD 10_1101-2021_01_07_425737 102 24 , , , 10_1101-2021_01_07_425737 102 25 ” " '' 10_1101-2021_01_07_425737 102 26 “ " `` 10_1101-2021_01_07_425737 102 27 Spin Spin NNP 10_1101-2021_01_07_425737 102 28 3 3 CD 10_1101-2021_01_07_425737 102 29 , , , 10_1101-2021_01_07_425737 102 30 ” " '' 10_1101-2021_01_07_425737 102 31 “ " `` 10_1101-2021_01_07_425737 102 32 Final final JJ 10_1101-2021_01_07_425737 102 33 ” " '' 10_1101-2021_01_07_425737 102 34 ) ) -RRB- 10_1101-2021_01_07_425737 102 35 were be VBD 10_1101-2021_01_07_425737 102 36 cut cut VBN 10_1101-2021_01_07_425737 102 37 out out RP 10_1101-2021_01_07_425737 102 38 and and CC 10_1101-2021_01_07_425737 102 39 submitted submit VBN 10_1101-2021_01_07_425737 102 40 to to IN 10_1101-2021_01_07_425737 102 41 the the DT 10_1101-2021_01_07_425737 102 42 University University NNP 10_1101-2021_01_07_425737 102 43 of of IN 10_1101-2021_01_07_425737 102 44 Texas Texas NNP 10_1101-2021_01_07_425737 102 45 at at IN 10_1101-2021_01_07_425737 102 46 Austin Austin NNP 10_1101-2021_01_07_425737 102 47 CBRS CBRS NNP 10_1101-2021_01_07_425737 102 48 Biological Biological NNP 10_1101-2021_01_07_425737 102 49 Mass Mass NNP 10_1101-2021_01_07_425737 102 50 Spectrometry Spectrometry NNP 10_1101-2021_01_07_425737 102 51 Facility Facility NNP 10_1101-2021_01_07_425737 102 52 for for IN 10_1101-2021_01_07_425737 102 53 LC LC NNP 10_1101-2021_01_07_425737 102 54 - - HYPH 10_1101-2021_01_07_425737 102 55 MS MS NNP 10_1101-2021_01_07_425737 102 56 / / SYM 10_1101-2021_01_07_425737 102 57 MS MS NNP 10_1101-2021_01_07_425737 102 58 using use VBG 10_1101-2021_01_07_425737 102 59 a a DT 10_1101-2021_01_07_425737 102 60 Dionex Dionex NNP 10_1101-2021_01_07_425737 102 61 Ultimate Ultimate NNP 10_1101-2021_01_07_425737 102 62 3000 3000 CD 10_1101-2021_01_07_425737 102 63 RSLCnano rslcnano NN 10_1101-2021_01_07_425737 102 64 LC LC NNP 10_1101-2021_01_07_425737 102 65 coupled couple VBN 10_1101-2021_01_07_425737 102 66 to to IN 10_1101-2021_01_07_425737 102 67 a a DT 10_1101-2021_01_07_425737 102 68 Thermo Thermo NNP 10_1101-2021_01_07_425737 102 69 Orbitrap Orbitrap NNP 10_1101-2021_01_07_425737 102 70 Fusion Fusion NNP 10_1101-2021_01_07_425737 102 71 ( ( -LRB- 10_1101-2021_01_07_425737 102 72 Thermo Thermo NNP 10_1101-2021_01_07_425737 102 73 - - HYPH 10_1101-2021_01_07_425737 102 74 Fisher Fisher NNP 10_1101-2021_01_07_425737 102 75 ) ) -RRB- 10_1101-2021_01_07_425737 102 76 . . . 10_1101-2021_01_07_425737 103 1 Samples sample NNS 10_1101-2021_01_07_425737 103 2 were be VBD 10_1101-2021_01_07_425737 103 3 submitted submit VBN 10_1101-2021_01_07_425737 103 4 in in IN 10_1101-2021_01_07_425737 103 5 50mL 50mL NNP 10_1101-2021_01_07_425737 103 6 destain destain NN 10_1101-2021_01_07_425737 103 7 with with IN 10_1101-2021_01_07_425737 103 8 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 103 9 aphidicola aphidicola NNP 10_1101-2021_01_07_425737 103 10 str str NN 10_1101-2021_01_07_425737 103 11 . . . 10_1101-2021_01_07_425737 104 1 APS APS NNP 10_1101-2021_01_07_425737 104 2 provided provide VBD 10_1101-2021_01_07_425737 104 3 as as IN 10_1101-2021_01_07_425737 104 4 the the DT 10_1101-2021_01_07_425737 104 5 reference reference NN 10_1101-2021_01_07_425737 104 6 organism organism NN 10_1101-2021_01_07_425737 104 7 ( ( -LRB- 10_1101-2021_01_07_425737 104 8 ASM960v1 asm960v1 CD 10_1101-2021_01_07_425737 104 9 ) ) -RRB- 10_1101-2021_01_07_425737 104 10 . . . 10_1101-2021_01_07_425737 105 1 Prior prior RB 10_1101-2021_01_07_425737 105 2 to to IN 10_1101-2021_01_07_425737 105 3 HPLC hplc NN 10_1101-2021_01_07_425737 105 4 separation separation NN 10_1101-2021_01_07_425737 105 5 , , , 10_1101-2021_01_07_425737 105 6 peptides peptide NNS 10_1101-2021_01_07_425737 105 7 were be VBD 10_1101-2021_01_07_425737 105 8 desalted desalt VBN 10_1101-2021_01_07_425737 105 9 using use VBG 10_1101-2021_01_07_425737 105 10 Millipore Millipore NNP 10_1101-2021_01_07_425737 105 11 U U NNP 10_1101-2021_01_07_425737 105 12 - - HYPH 10_1101-2021_01_07_425737 105 13 C18 C18 NNP 10_1101-2021_01_07_425737 105 14 ZipTip ZipTip NNP 10_1101-2021_01_07_425737 105 15 Pipette Pipette NNP 10_1101-2021_01_07_425737 105 16 Tips Tips NNP 10_1101-2021_01_07_425737 105 17 ( ( -LRB- 10_1101-2021_01_07_425737 105 18 Millipore Millipore NNP 10_1101-2021_01_07_425737 105 19 - - HYPH 10_1101-2021_01_07_425737 105 20 Sigma Sigma NNP 10_1101-2021_01_07_425737 105 21 ) ) -RRB- 10_1101-2021_01_07_425737 105 22 . . . 10_1101-2021_01_07_425737 106 1 A a DT 10_1101-2021_01_07_425737 106 2 2 2 CD 10_1101-2021_01_07_425737 106 3 cm cm NN 10_1101-2021_01_07_425737 106 4 long long JJ 10_1101-2021_01_07_425737 106 5 x x SYM 10_1101-2021_01_07_425737 106 6 75μm 75μm JJ 10_1101-2021_01_07_425737 106 7 ID ID NNP 10_1101-2021_01_07_425737 106 8 C18 C18 NNP 10_1101-2021_01_07_425737 106 9 trap trap NN 10_1101-2021_01_07_425737 106 10 column column NN 10_1101-2021_01_07_425737 106 11 was be VBD 10_1101-2021_01_07_425737 106 12 followed follow VBN 10_1101-2021_01_07_425737 106 13 by by IN 10_1101-2021_01_07_425737 106 14 a a DT 10_1101-2021_01_07_425737 106 15 25 25 CD 10_1101-2021_01_07_425737 106 16 cm cm NNS 10_1101-2021_01_07_425737 106 17 long long JJ 10_1101-2021_01_07_425737 106 18 x x SYM 10_1101-2021_01_07_425737 106 19 75μm 75μm JJ 10_1101-2021_01_07_425737 106 20 analytical analytical JJ 10_1101-2021_01_07_425737 106 21 columns column NNS 10_1101-2021_01_07_425737 106 22 packed pack VBN 10_1101-2021_01_07_425737 106 23 with with IN 10_1101-2021_01_07_425737 106 24 C18 C18 NNP 10_1101-2021_01_07_425737 106 25 3μm 3μm NN 10_1101-2021_01_07_425737 106 26 material material NN 10_1101-2021_01_07_425737 106 27 ( ( -LRB- 10_1101-2021_01_07_425737 106 28 Thermo Thermo NNP 10_1101-2021_01_07_425737 106 29 Acclaim Acclaim NNP 10_1101-2021_01_07_425737 106 30 PepMap PepMap NNP 10_1101-2021_01_07_425737 106 31 100 100 CD 10_1101-2021_01_07_425737 106 32 , , , 10_1101-2021_01_07_425737 106 33 Thermo Thermo NNP 10_1101-2021_01_07_425737 106 34 - - HYPH 10_1101-2021_01_07_425737 106 35 Fisher Fisher NNP 10_1101-2021_01_07_425737 106 36 ) ) -RRB- 10_1101-2021_01_07_425737 106 37 running run VBG 10_1101-2021_01_07_425737 106 38 a a DT 10_1101-2021_01_07_425737 106 39 gradient gradient NN 10_1101-2021_01_07_425737 106 40 from from IN 10_1101-2021_01_07_425737 106 41 5 5 CD 10_1101-2021_01_07_425737 106 42 - - SYM 10_1101-2021_01_07_425737 106 43 35 35 CD 10_1101-2021_01_07_425737 106 44 % % NN 10_1101-2021_01_07_425737 106 45 . . . 10_1101-2021_01_07_425737 107 1 The the DT 10_1101-2021_01_07_425737 107 2 FT FT NNP 10_1101-2021_01_07_425737 107 3 - - HYPH 10_1101-2021_01_07_425737 107 4 MS MS NNP 10_1101-2021_01_07_425737 107 5 resolution resolution NN 10_1101-2021_01_07_425737 107 6 was be VBD 10_1101-2021_01_07_425737 107 7 set set VBN 10_1101-2021_01_07_425737 107 8 to to IN 10_1101-2021_01_07_425737 107 9 120,000 120,000 CD 10_1101-2021_01_07_425737 107 10 , , , 10_1101-2021_01_07_425737 107 11 with with IN 10_1101-2021_01_07_425737 107 12 an an DT 10_1101-2021_01_07_425737 107 13 MS MS NNP 10_1101-2021_01_07_425737 107 14 / / SYM 10_1101-2021_01_07_425737 107 15 MS MS NNP 10_1101-2021_01_07_425737 107 16 cycle cycle NN 10_1101-2021_01_07_425737 107 17 time time NN 10_1101-2021_01_07_425737 107 18 of of IN 10_1101-2021_01_07_425737 107 19 3 3 CD 10_1101-2021_01_07_425737 107 20 seconds second NNS 10_1101-2021_01_07_425737 107 21 and and CC 10_1101-2021_01_07_425737 107 22 acquisition acquisition NN 10_1101-2021_01_07_425737 107 23 in in IN 10_1101-2021_01_07_425737 107 24 HCD HCD NNP 10_1101-2021_01_07_425737 107 25 ion ion NN 10_1101-2021_01_07_425737 107 26 trap trap NN 10_1101-2021_01_07_425737 107 27 mode mode NN 10_1101-2021_01_07_425737 107 28 . . . 10_1101-2021_01_07_425737 108 1 Raw raw JJ 10_1101-2021_01_07_425737 108 2 data datum NNS 10_1101-2021_01_07_425737 108 3 was be VBD 10_1101-2021_01_07_425737 108 4 processed process VBN 10_1101-2021_01_07_425737 108 5 using use VBG 10_1101-2021_01_07_425737 108 6 SEQUEST SEQUEST NNP 10_1101-2021_01_07_425737 108 7 HT HT NNP 10_1101-2021_01_07_425737 108 8 embedded embed VBN 10_1101-2021_01_07_425737 108 9 in in IN 10_1101-2021_01_07_425737 108 10 Proteome Proteome NNP 10_1101-2021_01_07_425737 108 11 Discoverer Discoverer NNP 10_1101-2021_01_07_425737 108 12 ( ( -LRB- 10_1101-2021_01_07_425737 108 13 Thermo Thermo NNP 10_1101-2021_01_07_425737 108 14 - - HYPH 10_1101-2021_01_07_425737 108 15 Fisher Fisher NNP 10_1101-2021_01_07_425737 108 16 ) ) -RRB- 10_1101-2021_01_07_425737 108 17 . . . 10_1101-2021_01_07_425737 109 1 Scaffold Scaffold NNP 10_1101-2021_01_07_425737 109 2 4 4 CD 10_1101-2021_01_07_425737 109 3 ( ( -LRB- 10_1101-2021_01_07_425737 109 4 Proteome Proteome NNP 10_1101-2021_01_07_425737 109 5 software software NN 10_1101-2021_01_07_425737 109 6 ) ) -RRB- 10_1101-2021_01_07_425737 109 7 was be VBD 10_1101-2021_01_07_425737 109 8 used use VBN 10_1101-2021_01_07_425737 109 9 for for IN 10_1101-2021_01_07_425737 109 10 validation validation NN 10_1101-2021_01_07_425737 109 11 of of IN 10_1101-2021_01_07_425737 109 12 peptide peptide NN 10_1101-2021_01_07_425737 109 13 and and CC 10_1101-2021_01_07_425737 109 14 protein protein NN 10_1101-2021_01_07_425737 109 15 IDs IDs NNP 10_1101-2021_01_07_425737 109 16 . . . 10_1101-2021_01_07_425737 110 1 EM em NN 10_1101-2021_01_07_425737 110 2 and and CC 10_1101-2021_01_07_425737 110 3 data datum NNS 10_1101-2021_01_07_425737 110 4 collection collection NN 10_1101-2021_01_07_425737 110 5 Protein protein NN 10_1101-2021_01_07_425737 110 6 from from IN 10_1101-2021_01_07_425737 110 7 the the DT 10_1101-2021_01_07_425737 110 8 final final JJ 10_1101-2021_01_07_425737 110 9 step step NN 10_1101-2021_01_07_425737 110 10 of of IN 10_1101-2021_01_07_425737 110 11 this this DT 10_1101-2021_01_07_425737 110 12 procedure procedure NN 10_1101-2021_01_07_425737 110 13 was be VBD 10_1101-2021_01_07_425737 110 14 stained stain VBN 10_1101-2021_01_07_425737 110 15 using use VBG 10_1101-2021_01_07_425737 110 16 3 3 CD 10_1101-2021_01_07_425737 110 17 % % NN 10_1101-2021_01_07_425737 110 18 Uranyl Uranyl NNP 10_1101-2021_01_07_425737 110 19 Acetate Acetate NNP 10_1101-2021_01_07_425737 110 20 on on IN 10_1101-2021_01_07_425737 110 21 a a DT 10_1101-2021_01_07_425737 110 22 400-mesh 400-mesh CD 10_1101-2021_01_07_425737 110 23 continuous continuous JJ 10_1101-2021_01_07_425737 110 24 carbon carbon NN 10_1101-2021_01_07_425737 110 25 grid grid NN 10_1101-2021_01_07_425737 110 26 . . . 10_1101-2021_01_07_425737 111 1 Images image NNS 10_1101-2021_01_07_425737 111 2 were be VBD 10_1101-2021_01_07_425737 111 3 acquired acquire VBN 10_1101-2021_01_07_425737 111 4 using use VBG 10_1101-2021_01_07_425737 111 5 an an DT 10_1101-2021_01_07_425737 111 6 FEI FEI NNP 10_1101-2021_01_07_425737 111 7 Talos Talos NNP 10_1101-2021_01_07_425737 111 8 transmission transmission NN 10_1101-2021_01_07_425737 111 9 ( ( -LRB- 10_1101-2021_01_07_425737 111 10 which which WDT 10_1101-2021_01_07_425737 111 11 was be VBD 10_1101-2021_01_07_425737 111 12 not not RB 10_1101-2021_01_07_425737 111 13 certified certify VBN 10_1101-2021_01_07_425737 111 14 by by IN 10_1101-2021_01_07_425737 111 15 peer peer NN 10_1101-2021_01_07_425737 111 16 review review NN 10_1101-2021_01_07_425737 111 17 ) ) -RRB- 10_1101-2021_01_07_425737 111 18 is be VBZ 10_1101-2021_01_07_425737 111 19 the the DT 10_1101-2021_01_07_425737 111 20 author author NN 10_1101-2021_01_07_425737 111 21 / / SYM 10_1101-2021_01_07_425737 111 22 funder funder NN 10_1101-2021_01_07_425737 111 23 . . . 10_1101-2021_01_07_425737 112 1 All all DT 10_1101-2021_01_07_425737 112 2 rights right NNS 10_1101-2021_01_07_425737 112 3 reserved reserve VBD 10_1101-2021_01_07_425737 112 4 . . . 10_1101-2021_01_07_425737 113 1 No no DT 10_1101-2021_01_07_425737 113 2 reuse reuse NN 10_1101-2021_01_07_425737 113 3 allowed allow VBN 10_1101-2021_01_07_425737 113 4 without without IN 10_1101-2021_01_07_425737 113 5 permission permission NN 10_1101-2021_01_07_425737 113 6 . . . 10_1101-2021_01_07_425737 114 1 The the DT 10_1101-2021_01_07_425737 114 2 copyright copyright NN 10_1101-2021_01_07_425737 114 3 holder holder NN 10_1101-2021_01_07_425737 114 4 for for IN 10_1101-2021_01_07_425737 114 5 this this DT 10_1101-2021_01_07_425737 114 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 114 7 version version NN 10_1101-2021_01_07_425737 114 8 posted post VBD 10_1101-2021_01_07_425737 114 9 January January NNP 10_1101-2021_01_07_425737 114 10 8 8 CD 10_1101-2021_01_07_425737 114 11 , , , 10_1101-2021_01_07_425737 114 12 2021 2021 CD 10_1101-2021_01_07_425737 114 13 . . . 10_1101-2021_01_07_425737 114 14 ; ; : 10_1101-2021_01_07_425737 114 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 114 16 : : : 10_1101-2021_01_07_425737 114 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 114 18 preprint preprint NN 10_1101-2021_01_07_425737 114 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 114 20 electron electron NNP 10_1101-2021_01_07_425737 114 21 microscope microscope NN 10_1101-2021_01_07_425737 114 22 operating operate VBG 10_1101-2021_01_07_425737 114 23 at at IN 10_1101-2021_01_07_425737 114 24 200 200 CD 10_1101-2021_01_07_425737 114 25 kV kV NNP 10_1101-2021_01_07_425737 114 26 , , , 10_1101-2021_01_07_425737 114 27 with with IN 10_1101-2021_01_07_425737 114 28 1.25 1.25 CD 10_1101-2021_01_07_425737 114 29 second second JJ 10_1101-2021_01_07_425737 114 30 exposures exposure NNS 10_1101-2021_01_07_425737 114 31 , , , 10_1101-2021_01_07_425737 114 32 a a DT 10_1101-2021_01_07_425737 114 33 dose dose NN 10_1101-2021_01_07_425737 114 34 rate rate NN 10_1101-2021_01_07_425737 114 35 of of IN 10_1101-2021_01_07_425737 114 36 19 19 CD 10_1101-2021_01_07_425737 114 37 e e CD 10_1101-2021_01_07_425737 114 38 - - HYPH 10_1101-2021_01_07_425737 114 39 Å-2 Å-2 NNP 10_1101-2021_01_07_425737 114 40 , , , 10_1101-2021_01_07_425737 114 41 and and CC 10_1101-2021_01_07_425737 114 42 a a DT 10_1101-2021_01_07_425737 114 43 nominal nominal JJ 10_1101-2021_01_07_425737 114 44 magnification magnification NN 10_1101-2021_01_07_425737 114 45 of of IN 10_1101-2021_01_07_425737 114 46 57,000X. 57,000X. NNP 10_1101-2021_01_07_425737 115 1 Whole whole JJ 10_1101-2021_01_07_425737 115 2 aphid aphid NN 10_1101-2021_01_07_425737 115 3 proteomic proteomic NN 10_1101-2021_01_07_425737 115 4 samples sample NNS 10_1101-2021_01_07_425737 115 5 For for IN 10_1101-2021_01_07_425737 115 6 controls control NNS 10_1101-2021_01_07_425737 115 7 , , , 10_1101-2021_01_07_425737 115 8 proteomes proteome NNS 10_1101-2021_01_07_425737 115 9 were be VBD 10_1101-2021_01_07_425737 115 10 profiled profile VBN 10_1101-2021_01_07_425737 115 11 for for IN 10_1101-2021_01_07_425737 115 12 whole whole JJ 10_1101-2021_01_07_425737 115 13 aphids aphid NNS 10_1101-2021_01_07_425737 115 14 , , , 10_1101-2021_01_07_425737 115 15 including include VBG 10_1101-2021_01_07_425737 115 16 both both CC 10_1101-2021_01_07_425737 115 17 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 115 18 and and CC 10_1101-2021_01_07_425737 115 19 aphid aphid NN 10_1101-2021_01_07_425737 115 20 cells cell NNS 10_1101-2021_01_07_425737 115 21 . . . 10_1101-2021_01_07_425737 116 1 Aphids aphid NNS 10_1101-2021_01_07_425737 116 2 were be VBD 10_1101-2021_01_07_425737 116 3 mixed mix VBN 10_1101-2021_01_07_425737 116 4 - - HYPH 10_1101-2021_01_07_425737 116 5 aged aged JJ 10_1101-2021_01_07_425737 116 6 populations population NNS 10_1101-2021_01_07_425737 116 7 grown grow VBN 10_1101-2021_01_07_425737 116 8 at at IN 10_1101-2021_01_07_425737 116 9 20ºC 20ºc CD 10_1101-2021_01_07_425737 116 10 in in IN 10_1101-2021_01_07_425737 116 11 30 30 CD 10_1101-2021_01_07_425737 116 12 cup cup NN 10_1101-2021_01_07_425737 116 13 cages cage NNS 10_1101-2021_01_07_425737 116 14 and and CC 10_1101-2021_01_07_425737 116 15 pooled pool VBD 10_1101-2021_01_07_425737 116 16 into into IN 10_1101-2021_01_07_425737 116 17 three three CD 10_1101-2021_01_07_425737 116 18 replicate replicate NN 10_1101-2021_01_07_425737 116 19 samples sample NNS 10_1101-2021_01_07_425737 116 20 . . . 10_1101-2021_01_07_425737 117 1 Aphids aphid NNS 10_1101-2021_01_07_425737 117 2 were be VBD 10_1101-2021_01_07_425737 117 3 washed wash VBN 10_1101-2021_01_07_425737 117 4 and and CC 10_1101-2021_01_07_425737 117 5 homogenized homogenize VBN 10_1101-2021_01_07_425737 117 6 in in IN 10_1101-2021_01_07_425737 117 7 buffer buffer NN 10_1101-2021_01_07_425737 117 8 as as IN 10_1101-2021_01_07_425737 117 9 described describe VBN 10_1101-2021_01_07_425737 117 10 above above RB 10_1101-2021_01_07_425737 117 11 . . . 10_1101-2021_01_07_425737 118 1 The the DT 10_1101-2021_01_07_425737 118 2 homogenate homogenate NN 10_1101-2021_01_07_425737 118 3 was be VBD 10_1101-2021_01_07_425737 118 4 centrifuged centrifuge VBN 10_1101-2021_01_07_425737 118 5 at at IN 10_1101-2021_01_07_425737 118 6 4000 4000 CD 10_1101-2021_01_07_425737 118 7 g g NNP 10_1101-2021_01_07_425737 118 8 for for IN 10_1101-2021_01_07_425737 118 9 15min 15min NNS 10_1101-2021_01_07_425737 118 10 at at IN 10_1101-2021_01_07_425737 118 11 4ºC 4ºc CD 10_1101-2021_01_07_425737 118 12 , , , 10_1101-2021_01_07_425737 118 13 Supernatant Supernatant NNP 10_1101-2021_01_07_425737 118 14 was be VBD 10_1101-2021_01_07_425737 118 15 removed remove VBN 10_1101-2021_01_07_425737 118 16 , , , 10_1101-2021_01_07_425737 118 17 and and CC 10_1101-2021_01_07_425737 118 18 pellet pellet NNP 10_1101-2021_01_07_425737 118 19 was be VBD 10_1101-2021_01_07_425737 118 20 suspended suspend VBN 10_1101-2021_01_07_425737 118 21 with with IN 10_1101-2021_01_07_425737 118 22 2 2 CD 10_1101-2021_01_07_425737 118 23 % % NN 10_1101-2021_01_07_425737 118 24 SDS sds NN 10_1101-2021_01_07_425737 118 25 , , , 10_1101-2021_01_07_425737 118 26 0.1 0.1 CD 10_1101-2021_01_07_425737 118 27 M M NNP 10_1101-2021_01_07_425737 118 28 Tris Tris NNP 10_1101-2021_01_07_425737 118 29 - - HYPH 10_1101-2021_01_07_425737 118 30 HCl HCl NNP 10_1101-2021_01_07_425737 118 31 , , , 10_1101-2021_01_07_425737 118 32 0.1 0.1 CD 10_1101-2021_01_07_425737 118 33 M M NNP 10_1101-2021_01_07_425737 118 34 DTT DTT NNP 10_1101-2021_01_07_425737 118 35 at at IN 10_1101-2021_01_07_425737 118 36 100oC 100oc NN 10_1101-2021_01_07_425737 118 37 for for IN 10_1101-2021_01_07_425737 118 38 10 10 CD 10_1101-2021_01_07_425737 118 39 min min NN 10_1101-2021_01_07_425737 118 40 , , , 10_1101-2021_01_07_425737 118 41 then then RB 10_1101-2021_01_07_425737 118 42 centrifuged centrifuge VBD 10_1101-2021_01_07_425737 118 43 at at IN 10_1101-2021_01_07_425737 118 44 14,000 14,000 CD 10_1101-2021_01_07_425737 118 45 g g NN 10_1101-2021_01_07_425737 118 46 for for IN 10_1101-2021_01_07_425737 118 47 20min 20min NNP 10_1101-2021_01_07_425737 118 48 at at IN 10_1101-2021_01_07_425737 118 49 4C 4C NNP 10_1101-2021_01_07_425737 118 50 to to TO 10_1101-2021_01_07_425737 118 51 remove remove VB 10_1101-2021_01_07_425737 118 52 non non JJ 10_1101-2021_01_07_425737 118 53 - - JJ 10_1101-2021_01_07_425737 118 54 soluble soluble JJ 10_1101-2021_01_07_425737 118 55 material material NN 10_1101-2021_01_07_425737 118 56 after after IN 10_1101-2021_01_07_425737 118 57 adding add VBG 10_1101-2021_01_07_425737 118 58 same same JJ 10_1101-2021_01_07_425737 118 59 volume volume NN 10_1101-2021_01_07_425737 118 60 of of IN 10_1101-2021_01_07_425737 118 61 8 8 CD 10_1101-2021_01_07_425737 118 62 M M NNP 10_1101-2021_01_07_425737 118 63 Urea Urea NNP 10_1101-2021_01_07_425737 118 64 . . . 10_1101-2021_01_07_425737 119 1 Protein protein NN 10_1101-2021_01_07_425737 119 2 concentration concentration NN 10_1101-2021_01_07_425737 119 3 was be VBD 10_1101-2021_01_07_425737 119 4 determined determine VBN 10_1101-2021_01_07_425737 119 5 on on IN 10_1101-2021_01_07_425737 119 6 an an DT 10_1101-2021_01_07_425737 119 7 Eppendorf Eppendorf NNP 10_1101-2021_01_07_425737 119 8 BioPhotometer biophotometer NN 10_1101-2021_01_07_425737 119 9 . . . 10_1101-2021_01_07_425737 120 1 5 5 CD 10_1101-2021_01_07_425737 120 2 mg mg NNP 10_1101-2021_01_07_425737 120 3 total total JJ 10_1101-2021_01_07_425737 120 4 protein protein NN 10_1101-2021_01_07_425737 120 5 was be VBD 10_1101-2021_01_07_425737 120 6 run run VBN 10_1101-2021_01_07_425737 120 7 on on IN 10_1101-2021_01_07_425737 120 8 a a DT 10_1101-2021_01_07_425737 120 9 Bis Bis NNP 10_1101-2021_01_07_425737 120 10 - - HYPH 10_1101-2021_01_07_425737 120 11 Tris Tris NNP 10_1101-2021_01_07_425737 120 12 gel gel NN 10_1101-2021_01_07_425737 120 13 for for IN 10_1101-2021_01_07_425737 120 14 less less JJR 10_1101-2021_01_07_425737 120 15 than than IN 10_1101-2021_01_07_425737 120 16 1 1 CD 10_1101-2021_01_07_425737 120 17 cm cm NNS 10_1101-2021_01_07_425737 120 18 , , , 10_1101-2021_01_07_425737 120 19 and and CC 10_1101-2021_01_07_425737 120 20 the the DT 10_1101-2021_01_07_425737 120 21 band band NN 10_1101-2021_01_07_425737 120 22 was be VBD 10_1101-2021_01_07_425737 120 23 excised excise VBN 10_1101-2021_01_07_425737 120 24 and and CC 10_1101-2021_01_07_425737 120 25 and and CC 10_1101-2021_01_07_425737 120 26 sent send VBN 10_1101-2021_01_07_425737 120 27 to to IN 10_1101-2021_01_07_425737 120 28 the the DT 10_1101-2021_01_07_425737 120 29 UT UT NNP 10_1101-2021_01_07_425737 120 30 Proteomics Proteomics NNP 10_1101-2021_01_07_425737 120 31 Core Core NNP 10_1101-2021_01_07_425737 120 32 for for IN 10_1101-2021_01_07_425737 120 33 LC LC NNP 10_1101-2021_01_07_425737 120 34 - - HYPH 10_1101-2021_01_07_425737 120 35 MS MS NNP 10_1101-2021_01_07_425737 120 36 / / SYM 10_1101-2021_01_07_425737 120 37 MS MS NNP 10_1101-2021_01_07_425737 120 38 protein protein NN 10_1101-2021_01_07_425737 120 39 ID ID NNP 10_1101-2021_01_07_425737 120 40 . . . 10_1101-2021_01_07_425737 121 1 Protein Protein NNP 10_1101-2021_01_07_425737 121 2 ID ID NNP 10_1101-2021_01_07_425737 121 3 methods method NNS 10_1101-2021_01_07_425737 121 4 were be VBD 10_1101-2021_01_07_425737 121 5 identical identical JJ 10_1101-2021_01_07_425737 121 6 as as IN 10_1101-2021_01_07_425737 121 7 detailed detail VBN 10_1101-2021_01_07_425737 121 8 above above RB 10_1101-2021_01_07_425737 121 9 . . . 10_1101-2021_01_07_425737 122 1 Author author NN 10_1101-2021_01_07_425737 122 2 contributions contribution NNS 10_1101-2021_01_07_425737 122 3 M.J.S. M.J.S. NNP 10_1101-2021_01_07_425737 123 1 raised raise VBD 10_1101-2021_01_07_425737 123 2 aphids aphids NNP 10_1101-2021_01_07_425737 123 3 and and CC 10_1101-2021_01_07_425737 123 4 prepared prepare VBD 10_1101-2021_01_07_425737 123 5 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 123 6 protein protein NN 10_1101-2021_01_07_425737 123 7 extracts extract NNS 10_1101-2021_01_07_425737 123 8 . . . 10_1101-2021_01_07_425737 124 1 J.N.Y. J.N.Y. NNP 10_1101-2021_01_07_425737 125 1 performed perform VBN 10_1101-2021_01_07_425737 125 2 electron electron NNP 10_1101-2021_01_07_425737 125 3 microscopy microscopy NNP 10_1101-2021_01_07_425737 125 4 . . . 10_1101-2021_01_07_425737 126 1 N.A.M. N.A.M. NNP 10_1101-2021_01_07_425737 127 1 and and CC 10_1101-2021_01_07_425737 127 2 D.W.T. D.W.T. NNP 10_1101-2021_01_07_425737 128 1 analyzed analyze VBN 10_1101-2021_01_07_425737 128 2 data datum NNS 10_1101-2021_01_07_425737 128 3 and and CC 10_1101-2021_01_07_425737 128 4 supervised supervise VBD 10_1101-2021_01_07_425737 128 5 and and CC 10_1101-2021_01_07_425737 128 6 secured secured JJ 10_1101-2021_01_07_425737 128 7 funding funding NN 10_1101-2021_01_07_425737 128 8 for for IN 10_1101-2021_01_07_425737 128 9 this this DT 10_1101-2021_01_07_425737 128 10 work work NN 10_1101-2021_01_07_425737 128 11 . . . 10_1101-2021_01_07_425737 129 1 All all DT 10_1101-2021_01_07_425737 129 2 authors author NNS 10_1101-2021_01_07_425737 129 3 reviewed review VBD 10_1101-2021_01_07_425737 129 4 the the DT 10_1101-2021_01_07_425737 129 5 final final JJ 10_1101-2021_01_07_425737 129 6 manuscript manuscript NN 10_1101-2021_01_07_425737 129 7 . . . 10_1101-2021_01_07_425737 130 1 Acknowledgements acknowledgement NNS 10_1101-2021_01_07_425737 130 2 We -PRON- PRP 10_1101-2021_01_07_425737 130 3 thank thank VBP 10_1101-2021_01_07_425737 130 4 Eric Eric NNP 10_1101-2021_01_07_425737 130 5 Verbeke Verbeke NNP 10_1101-2021_01_07_425737 130 6 , , , 10_1101-2021_01_07_425737 130 7 Jack Jack NNP 10_1101-2021_01_07_425737 130 8 Bravo Bravo NNP 10_1101-2021_01_07_425737 130 9 , , , 10_1101-2021_01_07_425737 130 10 and and CC 10_1101-2021_01_07_425737 130 11 Evan Evan NNP 10_1101-2021_01_07_425737 130 12 Schwartz Schwartz NNP 10_1101-2021_01_07_425737 130 13 for for IN 10_1101-2021_01_07_425737 130 14 their -PRON- PRP$ 10_1101-2021_01_07_425737 130 15 advice advice NN 10_1101-2021_01_07_425737 130 16 and and CC 10_1101-2021_01_07_425737 130 17 ideas idea NNS 10_1101-2021_01_07_425737 130 18 for for IN 10_1101-2021_01_07_425737 130 19 isolating isolate VBG 10_1101-2021_01_07_425737 130 20 and and CC 10_1101-2021_01_07_425737 130 21 imaging image VBG 10_1101-2021_01_07_425737 130 22 proteins protein NNS 10_1101-2021_01_07_425737 130 23 from from IN 10_1101-2021_01_07_425737 130 24 native native JJ 10_1101-2021_01_07_425737 130 25 cells cell NNS 10_1101-2021_01_07_425737 130 26 ; ; : 10_1101-2021_01_07_425737 130 27 Julie Julie NNP 10_1101-2021_01_07_425737 130 28 Perreau Perreau NNP 10_1101-2021_01_07_425737 130 29 , , , 10_1101-2021_01_07_425737 130 30 Margaret Margaret NNP 10_1101-2021_01_07_425737 130 31 Steele Steele NNP 10_1101-2021_01_07_425737 130 32 , , , 10_1101-2021_01_07_425737 130 33 and and CC 10_1101-2021_01_07_425737 130 34 Serena Serena NNP 10_1101-2021_01_07_425737 130 35 Zhao Zhao NNP 10_1101-2021_01_07_425737 130 36 for for IN 10_1101-2021_01_07_425737 130 37 creating create VBG 10_1101-2021_01_07_425737 130 38 a a DT 10_1101-2021_01_07_425737 130 39 space space NN 10_1101-2021_01_07_425737 130 40 in in IN 10_1101-2021_01_07_425737 130 41 which which WDT 10_1101-2021_01_07_425737 130 42 ideas idea NNS 10_1101-2021_01_07_425737 130 43 and and CC 10_1101-2021_01_07_425737 130 44 techniques technique NNS 10_1101-2021_01_07_425737 130 45 could could MD 10_1101-2021_01_07_425737 130 46 be be VB 10_1101-2021_01_07_425737 130 47 shared share VBN 10_1101-2021_01_07_425737 130 48 freely freely RB 10_1101-2021_01_07_425737 130 49 ; ; : 10_1101-2021_01_07_425737 130 50 Kim Kim NNP 10_1101-2021_01_07_425737 130 51 Hammond Hammond NNP 10_1101-2021_01_07_425737 130 52 for for IN 10_1101-2021_01_07_425737 130 53 help help NN 10_1101-2021_01_07_425737 130 54 with with IN 10_1101-2021_01_07_425737 130 55 aphid aphid NNP 10_1101-2021_01_07_425737 130 56 raising raising NN 10_1101-2021_01_07_425737 130 57 and and CC 10_1101-2021_01_07_425737 130 58 organization organization NN 10_1101-2021_01_07_425737 130 59 . . . 10_1101-2021_01_07_425737 131 1 This this DT 10_1101-2021_01_07_425737 131 2 work work NN 10_1101-2021_01_07_425737 131 3 was be VBD 10_1101-2021_01_07_425737 131 4 supported support VBN 10_1101-2021_01_07_425737 131 5 by by IN 10_1101-2021_01_07_425737 131 6 the the DT 10_1101-2021_01_07_425737 131 7 National National NNP 10_1101-2021_01_07_425737 131 8 Science Science NNP 10_1101-2021_01_07_425737 131 9 Foundation Foundation NNP 10_1101-2021_01_07_425737 131 10 1551092 1551092 CD 10_1101-2021_01_07_425737 131 11 ( ( -LRB- 10_1101-2021_01_07_425737 131 12 to to IN 10_1101-2021_01_07_425737 131 13 N.A.M N.A.M NNP 10_1101-2021_01_07_425737 131 14 ) ) -RRB- 10_1101-2021_01_07_425737 131 15 , , , 10_1101-2021_01_07_425737 131 16 a a DT 10_1101-2021_01_07_425737 131 17 Welch Welch NNP 10_1101-2021_01_07_425737 131 18 Foundation Foundation NNP 10_1101-2021_01_07_425737 131 19 grant grant NN 10_1101-2021_01_07_425737 131 20 F-1938 f-1938 NN 10_1101-2021_01_07_425737 131 21 ( ( -LRB- 10_1101-2021_01_07_425737 131 22 which which WDT 10_1101-2021_01_07_425737 131 23 was be VBD 10_1101-2021_01_07_425737 131 24 not not RB 10_1101-2021_01_07_425737 131 25 certified certify VBN 10_1101-2021_01_07_425737 131 26 by by IN 10_1101-2021_01_07_425737 131 27 peer peer NN 10_1101-2021_01_07_425737 131 28 review review NN 10_1101-2021_01_07_425737 131 29 ) ) -RRB- 10_1101-2021_01_07_425737 131 30 is be VBZ 10_1101-2021_01_07_425737 131 31 the the DT 10_1101-2021_01_07_425737 131 32 author author NN 10_1101-2021_01_07_425737 131 33 / / SYM 10_1101-2021_01_07_425737 131 34 funder funder NN 10_1101-2021_01_07_425737 131 35 . . . 10_1101-2021_01_07_425737 132 1 All all DT 10_1101-2021_01_07_425737 132 2 rights right NNS 10_1101-2021_01_07_425737 132 3 reserved reserve VBD 10_1101-2021_01_07_425737 132 4 . . . 10_1101-2021_01_07_425737 133 1 No no DT 10_1101-2021_01_07_425737 133 2 reuse reuse NN 10_1101-2021_01_07_425737 133 3 allowed allow VBN 10_1101-2021_01_07_425737 133 4 without without IN 10_1101-2021_01_07_425737 133 5 permission permission NN 10_1101-2021_01_07_425737 133 6 . . . 10_1101-2021_01_07_425737 134 1 The the DT 10_1101-2021_01_07_425737 134 2 copyright copyright NN 10_1101-2021_01_07_425737 134 3 holder holder NN 10_1101-2021_01_07_425737 134 4 for for IN 10_1101-2021_01_07_425737 134 5 this this DT 10_1101-2021_01_07_425737 134 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 134 7 version version NN 10_1101-2021_01_07_425737 134 8 posted post VBD 10_1101-2021_01_07_425737 134 9 January January NNP 10_1101-2021_01_07_425737 134 10 8 8 CD 10_1101-2021_01_07_425737 134 11 , , , 10_1101-2021_01_07_425737 134 12 2021 2021 CD 10_1101-2021_01_07_425737 134 13 . . . 10_1101-2021_01_07_425737 134 14 ; ; : 10_1101-2021_01_07_425737 134 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 134 16 : : : 10_1101-2021_01_07_425737 134 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 134 18 preprint preprint NN 10_1101-2021_01_07_425737 134 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 134 20 ( ( -LRB- 10_1101-2021_01_07_425737 134 21 to to IN 10_1101-2021_01_07_425737 134 22 D.W.T. D.W.T. NNP 10_1101-2021_01_07_425737 135 1 ) ) -RRB- 10_1101-2021_01_07_425737 135 2 , , , 10_1101-2021_01_07_425737 135 3 National National NNP 10_1101-2021_01_07_425737 135 4 Institute Institute NNP 10_1101-2021_01_07_425737 135 5 of of IN 10_1101-2021_01_07_425737 135 6 General General NNP 10_1101-2021_01_07_425737 135 7 Medical Medical NNP 10_1101-2021_01_07_425737 135 8 Sciences Sciences NNPS 10_1101-2021_01_07_425737 135 9 ( ( -LRB- 10_1101-2021_01_07_425737 135 10 NIGMS NIGMS NNP 10_1101-2021_01_07_425737 135 11 ) ) -RRB- 10_1101-2021_01_07_425737 135 12 of of IN 10_1101-2021_01_07_425737 135 13 the the DT 10_1101-2021_01_07_425737 135 14 National National NNP 10_1101-2021_01_07_425737 135 15 Institutes Institutes NNPS 10_1101-2021_01_07_425737 135 16 of of IN 10_1101-2021_01_07_425737 135 17 Health Health NNP 10_1101-2021_01_07_425737 135 18 ( ( -LRB- 10_1101-2021_01_07_425737 135 19 NIH NIH NNP 10_1101-2021_01_07_425737 135 20 ) ) -RRB- 10_1101-2021_01_07_425737 135 21 R35GM138348 R35GM138348 NNP 10_1101-2021_01_07_425737 135 22 ( ( -LRB- 10_1101-2021_01_07_425737 135 23 to to IN 10_1101-2021_01_07_425737 135 24 D.W.T. D.W.T. NNP 10_1101-2021_01_07_425737 136 1 ) ) -RRB- 10_1101-2021_01_07_425737 136 2 , , , 10_1101-2021_01_07_425737 136 3 Army Army NNP 10_1101-2021_01_07_425737 136 4 Research Research NNP 10_1101-2021_01_07_425737 136 5 Office Office NNP 10_1101-2021_01_07_425737 136 6 Grant Grant NNP 10_1101-2021_01_07_425737 136 7 W911NF-15 W911NF-15 NNP 10_1101-2021_01_07_425737 136 8 - - HYPH 10_1101-2021_01_07_425737 136 9 1 1 CD 10_1101-2021_01_07_425737 136 10 - - HYPH 10_1101-2021_01_07_425737 136 11 0120 0120 CD 10_1101-2021_01_07_425737 136 12 ( ( -LRB- 10_1101-2021_01_07_425737 136 13 to to IN 10_1101-2021_01_07_425737 136 14 D.W.T. D.W.T. NNP 10_1101-2021_01_07_425737 137 1 ) ) -RRB- 10_1101-2021_01_07_425737 137 2 , , , 10_1101-2021_01_07_425737 137 3 and and CC 10_1101-2021_01_07_425737 137 4 a a DT 10_1101-2021_01_07_425737 137 5 Robert Robert NNP 10_1101-2021_01_07_425737 137 6 J. J. NNP 10_1101-2021_01_07_425737 137 7 Kleberg Kleberg NNP 10_1101-2021_01_07_425737 137 8 , , , 10_1101-2021_01_07_425737 137 9 Jr. Jr. NNP 10_1101-2021_01_07_425737 137 10 and and CC 10_1101-2021_01_07_425737 137 11 Helen Helen NNP 10_1101-2021_01_07_425737 137 12 C. C. NNP 10_1101-2021_01_07_425737 137 13 Kleberg Kleberg NNP 10_1101-2021_01_07_425737 137 14 Foundation Foundation NNP 10_1101-2021_01_07_425737 137 15 Medical Medical NNP 10_1101-2021_01_07_425737 137 16 Research Research NNP 10_1101-2021_01_07_425737 137 17 Award Award NNP 10_1101-2021_01_07_425737 137 18 ( ( -LRB- 10_1101-2021_01_07_425737 137 19 to to IN 10_1101-2021_01_07_425737 137 20 D.W.T. D.W.T. NNP 10_1101-2021_01_07_425737 137 21 ) ) -RRB- 10_1101-2021_01_07_425737 137 22 . . . 10_1101-2021_01_07_425737 138 1 D.W.T D.W.T NNP 10_1101-2021_01_07_425737 138 2 is be VBZ 10_1101-2021_01_07_425737 138 3 a a DT 10_1101-2021_01_07_425737 138 4 CPRIT CPRIT NNP 10_1101-2021_01_07_425737 138 5 Scholar scholar NN 10_1101-2021_01_07_425737 138 6 supported support VBN 10_1101-2021_01_07_425737 138 7 by by IN 10_1101-2021_01_07_425737 138 8 the the DT 10_1101-2021_01_07_425737 138 9 Cancer Cancer NNP 10_1101-2021_01_07_425737 138 10 Prevention Prevention NNP 10_1101-2021_01_07_425737 138 11 and and CC 10_1101-2021_01_07_425737 138 12 Research Research NNP 10_1101-2021_01_07_425737 138 13 Institute Institute NNP 10_1101-2021_01_07_425737 138 14 of of IN 10_1101-2021_01_07_425737 138 15 Texas Texas NNP 10_1101-2021_01_07_425737 138 16 ( ( -LRB- 10_1101-2021_01_07_425737 138 17 RR160088 RR160088 NNP 10_1101-2021_01_07_425737 138 18 ) ) -RRB- 10_1101-2021_01_07_425737 138 19 and and CC 10_1101-2021_01_07_425737 138 20 an an DT 10_1101-2021_01_07_425737 138 21 Army Army NNP 10_1101-2021_01_07_425737 138 22 Young Young NNP 10_1101-2021_01_07_425737 138 23 Investigator Investigator NNP 10_1101-2021_01_07_425737 138 24 supported support VBN 10_1101-2021_01_07_425737 138 25 by by IN 10_1101-2021_01_07_425737 138 26 the the DT 10_1101-2021_01_07_425737 138 27 Army Army NNP 10_1101-2021_01_07_425737 138 28 Research Research NNP 10_1101-2021_01_07_425737 138 29 Office Office NNP 10_1101-2021_01_07_425737 138 30 ( ( -LRB- 10_1101-2021_01_07_425737 138 31 W911NF-19 W911NF-19 NNP 10_1101-2021_01_07_425737 138 32 - - HYPH 10_1101-2021_01_07_425737 138 33 1 1 CD 10_1101-2021_01_07_425737 138 34 - - HYPH 10_1101-2021_01_07_425737 138 35 0021 0021 CD 10_1101-2021_01_07_425737 138 36 ) ) -RRB- 10_1101-2021_01_07_425737 138 37 . . . 10_1101-2021_01_07_425737 139 1 Competing compete VBG 10_1101-2021_01_07_425737 139 2 interests interest NNS 10_1101-2021_01_07_425737 139 3 The the DT 10_1101-2021_01_07_425737 139 4 authors author NNS 10_1101-2021_01_07_425737 139 5 declare declare VBP 10_1101-2021_01_07_425737 139 6 no no DT 10_1101-2021_01_07_425737 139 7 competing compete VBG 10_1101-2021_01_07_425737 139 8 interests interest NNS 10_1101-2021_01_07_425737 139 9 . . . 10_1101-2021_01_07_425737 140 1 ( ( -LRB- 10_1101-2021_01_07_425737 140 2 which which WDT 10_1101-2021_01_07_425737 140 3 was be VBD 10_1101-2021_01_07_425737 140 4 not not RB 10_1101-2021_01_07_425737 140 5 certified certify VBN 10_1101-2021_01_07_425737 140 6 by by IN 10_1101-2021_01_07_425737 140 7 peer peer NN 10_1101-2021_01_07_425737 140 8 review review NN 10_1101-2021_01_07_425737 140 9 ) ) -RRB- 10_1101-2021_01_07_425737 140 10 is be VBZ 10_1101-2021_01_07_425737 140 11 the the DT 10_1101-2021_01_07_425737 140 12 author author NN 10_1101-2021_01_07_425737 140 13 / / SYM 10_1101-2021_01_07_425737 140 14 funder funder NN 10_1101-2021_01_07_425737 140 15 . . . 10_1101-2021_01_07_425737 141 1 All all DT 10_1101-2021_01_07_425737 141 2 rights right NNS 10_1101-2021_01_07_425737 141 3 reserved reserve VBD 10_1101-2021_01_07_425737 141 4 . . . 10_1101-2021_01_07_425737 142 1 No no DT 10_1101-2021_01_07_425737 142 2 reuse reuse NN 10_1101-2021_01_07_425737 142 3 allowed allow VBN 10_1101-2021_01_07_425737 142 4 without without IN 10_1101-2021_01_07_425737 142 5 permission permission NN 10_1101-2021_01_07_425737 142 6 . . . 10_1101-2021_01_07_425737 143 1 The the DT 10_1101-2021_01_07_425737 143 2 copyright copyright NN 10_1101-2021_01_07_425737 143 3 holder holder NN 10_1101-2021_01_07_425737 143 4 for for IN 10_1101-2021_01_07_425737 143 5 this this DT 10_1101-2021_01_07_425737 143 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 143 7 version version NN 10_1101-2021_01_07_425737 143 8 posted post VBD 10_1101-2021_01_07_425737 143 9 January January NNP 10_1101-2021_01_07_425737 143 10 8 8 CD 10_1101-2021_01_07_425737 143 11 , , , 10_1101-2021_01_07_425737 143 12 2021 2021 CD 10_1101-2021_01_07_425737 143 13 . . . 10_1101-2021_01_07_425737 143 14 ; ; : 10_1101-2021_01_07_425737 143 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 143 16 : : : 10_1101-2021_01_07_425737 143 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 143 18 preprint preprint NN 10_1101-2021_01_07_425737 143 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 143 20 Figures Figures NNP 10_1101-2021_01_07_425737 143 21 Figure Figure NNP 10_1101-2021_01_07_425737 143 22 1 1 CD 10_1101-2021_01_07_425737 143 23 : : : 10_1101-2021_01_07_425737 143 24 Barplot Barplot NNP 10_1101-2021_01_07_425737 143 25 showing show VBG 10_1101-2021_01_07_425737 143 26 flagellum flagellum JJ 10_1101-2021_01_07_425737 143 27 protein protein NN 10_1101-2021_01_07_425737 143 28 enrichment enrichment NN 10_1101-2021_01_07_425737 143 29 before before IN 10_1101-2021_01_07_425737 143 30 ( ( -LRB- 10_1101-2021_01_07_425737 143 31 Lysate Lysate NNP 10_1101-2021_01_07_425737 143 32 ) ) -RRB- 10_1101-2021_01_07_425737 143 33 and and CC 10_1101-2021_01_07_425737 143 34 after after IN 10_1101-2021_01_07_425737 143 35 ( ( -LRB- 10_1101-2021_01_07_425737 143 36 Final Final NNP 10_1101-2021_01_07_425737 143 37 ) ) -RRB- 10_1101-2021_01_07_425737 143 38 the the DT 10_1101-2021_01_07_425737 143 39 isolation isolation NN 10_1101-2021_01_07_425737 143 40 procedure procedure NN 10_1101-2021_01_07_425737 143 41 compared compare VBN 10_1101-2021_01_07_425737 143 42 to to IN 10_1101-2021_01_07_425737 143 43 proteomic proteomic JJ 10_1101-2021_01_07_425737 143 44 datasets dataset NNS 10_1101-2021_01_07_425737 143 45 generated generate VBN 10_1101-2021_01_07_425737 143 46 with with IN 10_1101-2021_01_07_425737 143 47 whole whole JJ 10_1101-2021_01_07_425737 143 48 aphids aphid NNS 10_1101-2021_01_07_425737 143 49 and and CC 10_1101-2021_01_07_425737 143 50 dissected dissect VBN 10_1101-2021_01_07_425737 143 51 bacteriocytes bacteriocyte NNS 10_1101-2021_01_07_425737 143 52 . . . 10_1101-2021_01_07_425737 144 1 Blue Blue NNP 10_1101-2021_01_07_425737 144 2 indicates indicate VBZ 10_1101-2021_01_07_425737 144 3 “ " `` 10_1101-2021_01_07_425737 144 4 core core NN 10_1101-2021_01_07_425737 144 5 ” " '' 10_1101-2021_01_07_425737 144 6 proteins protein NNS 10_1101-2021_01_07_425737 144 7 required require VBN 10_1101-2021_01_07_425737 144 8 for for IN 10_1101-2021_01_07_425737 144 9 secretion secretion NN 10_1101-2021_01_07_425737 144 10 activity activity NN 10_1101-2021_01_07_425737 144 11 and and CC 10_1101-2021_01_07_425737 144 12 red red NN 10_1101-2021_01_07_425737 144 13 indicates indicate VBZ 10_1101-2021_01_07_425737 144 14 accessory accessory JJ 10_1101-2021_01_07_425737 144 15 proteins protein NNS 10_1101-2021_01_07_425737 144 16 maintained maintain VBN 10_1101-2021_01_07_425737 144 17 by by IN 10_1101-2021_01_07_425737 144 18 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 144 19 aphidicola aphidicola NNP 10_1101-2021_01_07_425737 144 20 in in IN 10_1101-2021_01_07_425737 144 21 pea pea NNP 10_1101-2021_01_07_425737 144 22 aphids aphids NNP 10_1101-2021_01_07_425737 144 23 . . . 10_1101-2021_01_07_425737 145 1 ( ( -LRB- 10_1101-2021_01_07_425737 145 2 which which WDT 10_1101-2021_01_07_425737 145 3 was be VBD 10_1101-2021_01_07_425737 145 4 not not RB 10_1101-2021_01_07_425737 145 5 certified certify VBN 10_1101-2021_01_07_425737 145 6 by by IN 10_1101-2021_01_07_425737 145 7 peer peer NN 10_1101-2021_01_07_425737 145 8 review review NN 10_1101-2021_01_07_425737 145 9 ) ) -RRB- 10_1101-2021_01_07_425737 145 10 is be VBZ 10_1101-2021_01_07_425737 145 11 the the DT 10_1101-2021_01_07_425737 145 12 author author NN 10_1101-2021_01_07_425737 145 13 / / SYM 10_1101-2021_01_07_425737 145 14 funder funder NN 10_1101-2021_01_07_425737 145 15 . . . 10_1101-2021_01_07_425737 146 1 All all DT 10_1101-2021_01_07_425737 146 2 rights right NNS 10_1101-2021_01_07_425737 146 3 reserved reserve VBD 10_1101-2021_01_07_425737 146 4 . . . 10_1101-2021_01_07_425737 147 1 No no DT 10_1101-2021_01_07_425737 147 2 reuse reuse NN 10_1101-2021_01_07_425737 147 3 allowed allow VBN 10_1101-2021_01_07_425737 147 4 without without IN 10_1101-2021_01_07_425737 147 5 permission permission NN 10_1101-2021_01_07_425737 147 6 . . . 10_1101-2021_01_07_425737 148 1 The the DT 10_1101-2021_01_07_425737 148 2 copyright copyright NN 10_1101-2021_01_07_425737 148 3 holder holder NN 10_1101-2021_01_07_425737 148 4 for for IN 10_1101-2021_01_07_425737 148 5 this this DT 10_1101-2021_01_07_425737 148 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 148 7 version version NN 10_1101-2021_01_07_425737 148 8 posted post VBD 10_1101-2021_01_07_425737 148 9 January January NNP 10_1101-2021_01_07_425737 148 10 8 8 CD 10_1101-2021_01_07_425737 148 11 , , , 10_1101-2021_01_07_425737 148 12 2021 2021 CD 10_1101-2021_01_07_425737 148 13 . . . 10_1101-2021_01_07_425737 148 14 ; ; : 10_1101-2021_01_07_425737 148 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 148 16 : : : 10_1101-2021_01_07_425737 148 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 148 18 preprint preprint NN 10_1101-2021_01_07_425737 148 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 148 20 Figure figure NN 10_1101-2021_01_07_425737 148 21 2 2 CD 10_1101-2021_01_07_425737 148 22 : : : 10_1101-2021_01_07_425737 148 23 Cartoon cartoon NN 10_1101-2021_01_07_425737 148 24 diagram diagram NN 10_1101-2021_01_07_425737 148 25 of of IN 10_1101-2021_01_07_425737 148 26 the the DT 10_1101-2021_01_07_425737 148 27 reduced reduce VBN 10_1101-2021_01_07_425737 148 28 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 148 29 aphidicola aphidicola NN 10_1101-2021_01_07_425737 148 30 ( ( -LRB- 10_1101-2021_01_07_425737 148 31 pea pea NNP 10_1101-2021_01_07_425737 148 32 aphid aphid NNP 10_1101-2021_01_07_425737 148 33 ) ) -RRB- 10_1101-2021_01_07_425737 148 34 flagellum flagellum NNP 10_1101-2021_01_07_425737 148 35 . . . 10_1101-2021_01_07_425737 149 1 Colors color NNS 10_1101-2021_01_07_425737 149 2 indicate indicate VBP 10_1101-2021_01_07_425737 149 3 enrichment enrichment NN 10_1101-2021_01_07_425737 149 4 status status NN 10_1101-2021_01_07_425737 149 5 of of IN 10_1101-2021_01_07_425737 149 6 individual individual JJ 10_1101-2021_01_07_425737 149 7 proteins protein NNS 10_1101-2021_01_07_425737 149 8 at at IN 10_1101-2021_01_07_425737 149 9 the the DT 10_1101-2021_01_07_425737 149 10 final final JJ 10_1101-2021_01_07_425737 149 11 step step NN 10_1101-2021_01_07_425737 149 12 of of IN 10_1101-2021_01_07_425737 149 13 the the DT 10_1101-2021_01_07_425737 149 14 procedure procedure NN 10_1101-2021_01_07_425737 149 15 , , , 10_1101-2021_01_07_425737 149 16 corresponding correspond VBG 10_1101-2021_01_07_425737 149 17 to to IN 10_1101-2021_01_07_425737 149 18 Figure figure NN 10_1101-2021_01_07_425737 149 19 1 1 CD 10_1101-2021_01_07_425737 149 20 . . . 10_1101-2021_01_07_425737 150 1 ( ( -LRB- 10_1101-2021_01_07_425737 150 2 which which WDT 10_1101-2021_01_07_425737 150 3 was be VBD 10_1101-2021_01_07_425737 150 4 not not RB 10_1101-2021_01_07_425737 150 5 certified certify VBN 10_1101-2021_01_07_425737 150 6 by by IN 10_1101-2021_01_07_425737 150 7 peer peer NN 10_1101-2021_01_07_425737 150 8 review review NN 10_1101-2021_01_07_425737 150 9 ) ) -RRB- 10_1101-2021_01_07_425737 150 10 is be VBZ 10_1101-2021_01_07_425737 150 11 the the DT 10_1101-2021_01_07_425737 150 12 author author NN 10_1101-2021_01_07_425737 150 13 / / SYM 10_1101-2021_01_07_425737 150 14 funder funder NN 10_1101-2021_01_07_425737 150 15 . . . 10_1101-2021_01_07_425737 151 1 All all DT 10_1101-2021_01_07_425737 151 2 rights right NNS 10_1101-2021_01_07_425737 151 3 reserved reserve VBD 10_1101-2021_01_07_425737 151 4 . . . 10_1101-2021_01_07_425737 152 1 No no DT 10_1101-2021_01_07_425737 152 2 reuse reuse NN 10_1101-2021_01_07_425737 152 3 allowed allow VBN 10_1101-2021_01_07_425737 152 4 without without IN 10_1101-2021_01_07_425737 152 5 permission permission NN 10_1101-2021_01_07_425737 152 6 . . . 10_1101-2021_01_07_425737 153 1 The the DT 10_1101-2021_01_07_425737 153 2 copyright copyright NN 10_1101-2021_01_07_425737 153 3 holder holder NN 10_1101-2021_01_07_425737 153 4 for for IN 10_1101-2021_01_07_425737 153 5 this this DT 10_1101-2021_01_07_425737 153 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 153 7 version version NN 10_1101-2021_01_07_425737 153 8 posted post VBD 10_1101-2021_01_07_425737 153 9 January January NNP 10_1101-2021_01_07_425737 153 10 8 8 CD 10_1101-2021_01_07_425737 153 11 , , , 10_1101-2021_01_07_425737 153 12 2021 2021 CD 10_1101-2021_01_07_425737 153 13 . . . 10_1101-2021_01_07_425737 153 14 ; ; : 10_1101-2021_01_07_425737 153 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 153 16 : : : 10_1101-2021_01_07_425737 153 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 153 18 preprint preprint NN 10_1101-2021_01_07_425737 153 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 153 20 Figure figure NN 10_1101-2021_01_07_425737 153 21 3 3 CD 10_1101-2021_01_07_425737 153 22 : : : 10_1101-2021_01_07_425737 153 23 Single single JJ 10_1101-2021_01_07_425737 153 24 particles particle NNS 10_1101-2021_01_07_425737 153 25 of of IN 10_1101-2021_01_07_425737 153 26 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 153 27 flagellum flagellum NN 10_1101-2021_01_07_425737 153 28 complexes complex NNS 10_1101-2021_01_07_425737 153 29 after after IN 10_1101-2021_01_07_425737 153 30 the the DT 10_1101-2021_01_07_425737 153 31 isolation isolation NN 10_1101-2021_01_07_425737 153 32 procedure procedure NN 10_1101-2021_01_07_425737 153 33 . . . 10_1101-2021_01_07_425737 154 1 Scale scale NN 10_1101-2021_01_07_425737 154 2 bars bar NNS 10_1101-2021_01_07_425737 154 3 represent represent VBP 10_1101-2021_01_07_425737 154 4 50 50 CD 10_1101-2021_01_07_425737 154 5 nm nm NN 10_1101-2021_01_07_425737 154 6 . . . 10_1101-2021_01_07_425737 155 1 ( ( -LRB- 10_1101-2021_01_07_425737 155 2 which which WDT 10_1101-2021_01_07_425737 155 3 was be VBD 10_1101-2021_01_07_425737 155 4 not not RB 10_1101-2021_01_07_425737 155 5 certified certify VBN 10_1101-2021_01_07_425737 155 6 by by IN 10_1101-2021_01_07_425737 155 7 peer peer NN 10_1101-2021_01_07_425737 155 8 review review NN 10_1101-2021_01_07_425737 155 9 ) ) -RRB- 10_1101-2021_01_07_425737 155 10 is be VBZ 10_1101-2021_01_07_425737 155 11 the the DT 10_1101-2021_01_07_425737 155 12 author author NN 10_1101-2021_01_07_425737 155 13 / / SYM 10_1101-2021_01_07_425737 155 14 funder funder NN 10_1101-2021_01_07_425737 155 15 . . . 10_1101-2021_01_07_425737 156 1 All all DT 10_1101-2021_01_07_425737 156 2 rights right NNS 10_1101-2021_01_07_425737 156 3 reserved reserve VBD 10_1101-2021_01_07_425737 156 4 . . . 10_1101-2021_01_07_425737 157 1 No no DT 10_1101-2021_01_07_425737 157 2 reuse reuse NN 10_1101-2021_01_07_425737 157 3 allowed allow VBN 10_1101-2021_01_07_425737 157 4 without without IN 10_1101-2021_01_07_425737 157 5 permission permission NN 10_1101-2021_01_07_425737 157 6 . . . 10_1101-2021_01_07_425737 158 1 The the DT 10_1101-2021_01_07_425737 158 2 copyright copyright NN 10_1101-2021_01_07_425737 158 3 holder holder NN 10_1101-2021_01_07_425737 158 4 for for IN 10_1101-2021_01_07_425737 158 5 this this DT 10_1101-2021_01_07_425737 158 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 158 7 version version NN 10_1101-2021_01_07_425737 158 8 posted post VBD 10_1101-2021_01_07_425737 158 9 January January NNP 10_1101-2021_01_07_425737 158 10 8 8 CD 10_1101-2021_01_07_425737 158 11 , , , 10_1101-2021_01_07_425737 158 12 2021 2021 CD 10_1101-2021_01_07_425737 158 13 . . . 10_1101-2021_01_07_425737 158 14 ; ; : 10_1101-2021_01_07_425737 158 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 158 16 : : : 10_1101-2021_01_07_425737 158 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 158 18 preprint preprint NN 10_1101-2021_01_07_425737 158 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 158 20 Supplementary Supplementary NNP 10_1101-2021_01_07_425737 158 21 Figure Figure NNP 10_1101-2021_01_07_425737 158 22 1 1 CD 10_1101-2021_01_07_425737 158 23 : : : 10_1101-2021_01_07_425737 158 24 Silver silver NN 10_1101-2021_01_07_425737 158 25 stained stain VBN 10_1101-2021_01_07_425737 158 26 SDS sds NN 10_1101-2021_01_07_425737 158 27 gel gel NN 10_1101-2021_01_07_425737 158 28 created create VBN 10_1101-2021_01_07_425737 158 29 after after IN 10_1101-2021_01_07_425737 158 30 the the DT 10_1101-2021_01_07_425737 158 31 enrichment enrichment NN 10_1101-2021_01_07_425737 158 32 procedure procedure NN 10_1101-2021_01_07_425737 158 33 was be VBD 10_1101-2021_01_07_425737 158 34 performed perform VBN 10_1101-2021_01_07_425737 158 35 . . . 10_1101-2021_01_07_425737 159 1 The the DT 10_1101-2021_01_07_425737 159 2 first first JJ 10_1101-2021_01_07_425737 159 3 lane lane NN 10_1101-2021_01_07_425737 159 4 is be VBZ 10_1101-2021_01_07_425737 159 5 taken take VBN 10_1101-2021_01_07_425737 159 6 directly directly RB 10_1101-2021_01_07_425737 159 7 from from IN 10_1101-2021_01_07_425737 159 8 the the DT 10_1101-2021_01_07_425737 159 9 enrichment enrichment NN 10_1101-2021_01_07_425737 159 10 preparation preparation NN 10_1101-2021_01_07_425737 159 11 after after IN 10_1101-2021_01_07_425737 159 12 overnight overnight JJ 10_1101-2021_01_07_425737 159 13 incubation incubation NN 10_1101-2021_01_07_425737 159 14 with with IN 10_1101-2021_01_07_425737 159 15 TET TET NNP 10_1101-2021_01_07_425737 159 16 buffer buffer NN 10_1101-2021_01_07_425737 159 17 . . . 10_1101-2021_01_07_425737 160 1 The the DT 10_1101-2021_01_07_425737 160 2 second second JJ 10_1101-2021_01_07_425737 160 3 lane lane NN 10_1101-2021_01_07_425737 160 4 is be VBZ 10_1101-2021_01_07_425737 160 5 after after IN 10_1101-2021_01_07_425737 160 6 concentrating concentrate VBG 10_1101-2021_01_07_425737 160 7 the the DT 10_1101-2021_01_07_425737 160 8 enriched enriched JJ 10_1101-2021_01_07_425737 160 9 proteins protein NNS 10_1101-2021_01_07_425737 160 10 to to IN 10_1101-2021_01_07_425737 160 11 1 1 CD 10_1101-2021_01_07_425737 160 12 mg mg NNP 10_1101-2021_01_07_425737 160 13 / / SYM 10_1101-2021_01_07_425737 160 14 mL. mL. NNP 10_1101-2021_01_07_425737 161 1 The the DT 10_1101-2021_01_07_425737 161 2 third third JJ 10_1101-2021_01_07_425737 161 3 lane lane NN 10_1101-2021_01_07_425737 161 4 is be VBZ 10_1101-2021_01_07_425737 161 5 concentrated concentrated JJ 10_1101-2021_01_07_425737 161 6 protein protein NN 10_1101-2021_01_07_425737 161 7 diluted dilute VBN 10_1101-2021_01_07_425737 161 8 to to IN 10_1101-2021_01_07_425737 161 9 0.5 0.5 CD 10_1101-2021_01_07_425737 161 10 mg mg CD 10_1101-2021_01_07_425737 161 11 / / SYM 10_1101-2021_01_07_425737 161 12 mL. mL. NNP 10_1101-2021_01_07_425737 162 1 Ladder ladder NN 10_1101-2021_01_07_425737 162 2 values value NNS 10_1101-2021_01_07_425737 162 3 represent represent VBP 10_1101-2021_01_07_425737 162 4 molecular molecular JJ 10_1101-2021_01_07_425737 162 5 weight weight NN 10_1101-2021_01_07_425737 162 6 in in IN 10_1101-2021_01_07_425737 162 7 kDa kDa NNS 10_1101-2021_01_07_425737 162 8 . . . 10_1101-2021_01_07_425737 163 1 Symbols symbol NNS 10_1101-2021_01_07_425737 163 2 correspond correspond VBP 10_1101-2021_01_07_425737 163 3 to to IN 10_1101-2021_01_07_425737 163 4 flagellar flagellar JJ 10_1101-2021_01_07_425737 163 5 protein protein NN 10_1101-2021_01_07_425737 163 6 molecular molecular JJ 10_1101-2021_01_07_425737 163 7 weight weight NN 10_1101-2021_01_07_425737 163 8 : : : 10_1101-2021_01_07_425737 163 9 * * NFP 10_1101-2021_01_07_425737 163 10 corresponds correspond VBZ 10_1101-2021_01_07_425737 163 11 to to IN 10_1101-2021_01_07_425737 163 12 FlhA FlhA NNP 10_1101-2021_01_07_425737 163 13 ( ( -LRB- 10_1101-2021_01_07_425737 163 14 78 78 CD 10_1101-2021_01_07_425737 163 15 kDa kDa NNS 10_1101-2021_01_07_425737 163 16 ) ) -RRB- 10_1101-2021_01_07_425737 163 17 . . . 10_1101-2021_01_07_425737 164 1 † † PRP 10_1101-2021_01_07_425737 164 2 corresponds correspond VBZ 10_1101-2021_01_07_425737 164 3 to to IN 10_1101-2021_01_07_425737 164 4 FliF FliF NNP 10_1101-2021_01_07_425737 164 5 ( ( -LRB- 10_1101-2021_01_07_425737 164 6 63 63 CD 10_1101-2021_01_07_425737 164 7 kDa kDa NNS 10_1101-2021_01_07_425737 164 8 ) ) -RRB- 10_1101-2021_01_07_425737 164 9 and and CC 10_1101-2021_01_07_425737 164 10 FlgK FlgK NNP 10_1101-2021_01_07_425737 164 11 ( ( -LRB- 10_1101-2021_01_07_425737 164 12 63 63 CD 10_1101-2021_01_07_425737 164 13 kDa kDa NNS 10_1101-2021_01_07_425737 164 14 ) ) -RRB- 10_1101-2021_01_07_425737 164 15 . . . 10_1101-2021_01_07_425737 165 1 º º LS 10_1101-2021_01_07_425737 165 2 corresponds correspond NNS 10_1101-2021_01_07_425737 165 3 to to IN 10_1101-2021_01_07_425737 165 4 FlgE FlgE NNP 10_1101-2021_01_07_425737 165 5 ( ( -LRB- 10_1101-2021_01_07_425737 165 6 45 45 CD 10_1101-2021_01_07_425737 165 7 kDa kDa NNS 10_1101-2021_01_07_425737 165 8 ) ) -RRB- 10_1101-2021_01_07_425737 165 9 , , , 10_1101-2021_01_07_425737 165 10 FliP FliP NNP 10_1101-2021_01_07_425737 165 11 ( ( -LRB- 10_1101-2021_01_07_425737 165 12 43kDa 43kda CD 10_1101-2021_01_07_425737 165 13 ) ) -RRB- 10_1101-2021_01_07_425737 165 14 , , , 10_1101-2021_01_07_425737 165 15 and and CC 10_1101-2021_01_07_425737 165 16 FlgI FlgI NNP 10_1101-2021_01_07_425737 165 17 ( ( -LRB- 10_1101-2021_01_07_425737 165 18 41 41 CD 10_1101-2021_01_07_425737 165 19 kDa kDa NNS 10_1101-2021_01_07_425737 165 20 ) ) -RRB- 10_1101-2021_01_07_425737 165 21 . . . 10_1101-2021_01_07_425737 166 1 ‡ ‡ NN 10_1101-2021_01_07_425737 166 2 corresponds correspond VBZ 10_1101-2021_01_07_425737 166 3 to to IN 10_1101-2021_01_07_425737 166 4 FliG FliG NNP 10_1101-2021_01_07_425737 166 5 ( ( -LRB- 10_1101-2021_01_07_425737 166 6 38kDa 38kda CD 10_1101-2021_01_07_425737 166 7 ) ) -RRB- 10_1101-2021_01_07_425737 166 8 and and CC 10_1101-2021_01_07_425737 166 9 FliM FliM NNP 10_1101-2021_01_07_425737 166 10 ( ( -LRB- 10_1101-2021_01_07_425737 166 11 37 37 CD 10_1101-2021_01_07_425737 166 12 kDa kDa NNS 10_1101-2021_01_07_425737 166 13 ) ) -RRB- 10_1101-2021_01_07_425737 166 14 . . . 10_1101-2021_01_07_425737 167 1 ∆ ∆ NN 10_1101-2021_01_07_425737 167 2 corresponds correspond VBZ 10_1101-2021_01_07_425737 167 3 to to IN 10_1101-2021_01_07_425737 167 4 FlgG FlgG NNP 10_1101-2021_01_07_425737 167 5 ( ( -LRB- 10_1101-2021_01_07_425737 167 6 28 28 CD 10_1101-2021_01_07_425737 167 7 kDa kDa NNS 10_1101-2021_01_07_425737 167 8 ) ) -RRB- 10_1101-2021_01_07_425737 167 9 , , , 10_1101-2021_01_07_425737 167 10 FlgF FlgF NNP 10_1101-2021_01_07_425737 167 11 ( ( -LRB- 10_1101-2021_01_07_425737 167 12 28 28 CD 10_1101-2021_01_07_425737 167 13 kDa kDa NNS 10_1101-2021_01_07_425737 167 14 ) ) -RRB- 10_1101-2021_01_07_425737 167 15 , , , 10_1101-2021_01_07_425737 167 16 FlgH FlgH NNP 10_1101-2021_01_07_425737 167 17 ( ( -LRB- 10_1101-2021_01_07_425737 167 18 26 26 CD 10_1101-2021_01_07_425737 167 19 kDa kDa NNS 10_1101-2021_01_07_425737 167 20 ) ) -RRB- 10_1101-2021_01_07_425737 167 21 , , , 10_1101-2021_01_07_425737 167 22 and and CC 10_1101-2021_01_07_425737 167 23 FliH FliH NNP 10_1101-2021_01_07_425737 167 24 ( ( -LRB- 10_1101-2021_01_07_425737 167 25 26kDa 26kda CD 10_1101-2021_01_07_425737 167 26 ) ) -RRB- 10_1101-2021_01_07_425737 167 27 . . . 10_1101-2021_01_07_425737 168 1 Ø Ø NNP 10_1101-2021_01_07_425737 168 2 corresponds correspond VBZ 10_1101-2021_01_07_425737 168 3 to to IN 10_1101-2021_01_07_425737 168 4 FlgB FlgB NNP 10_1101-2021_01_07_425737 168 5 ( ( -LRB- 10_1101-2021_01_07_425737 168 6 16 16 CD 10_1101-2021_01_07_425737 168 7 kDa kDa NNS 10_1101-2021_01_07_425737 168 8 ) ) -RRB- 10_1101-2021_01_07_425737 168 9 , , , 10_1101-2021_01_07_425737 168 10 FliN FliN NNP 10_1101-2021_01_07_425737 168 11 ( ( -LRB- 10_1101-2021_01_07_425737 168 12 15 15 CD 10_1101-2021_01_07_425737 168 13 kDa kDa NNS 10_1101-2021_01_07_425737 168 14 ) ) -RRB- 10_1101-2021_01_07_425737 168 15 , , , 10_1101-2021_01_07_425737 168 16 FlgC FlgC NNP 10_1101-2021_01_07_425737 168 17 ( ( -LRB- 10_1101-2021_01_07_425737 168 18 15 15 CD 10_1101-2021_01_07_425737 168 19 kDa kDa NNS 10_1101-2021_01_07_425737 168 20 ) ) -RRB- 10_1101-2021_01_07_425737 168 21 , , , 10_1101-2021_01_07_425737 168 22 and and CC 10_1101-2021_01_07_425737 168 23 FliE FliE NNP 10_1101-2021_01_07_425737 168 24 ( ( -LRB- 10_1101-2021_01_07_425737 168 25 11 11 CD 10_1101-2021_01_07_425737 168 26 kDa kDa NNS 10_1101-2021_01_07_425737 168 27 ) ) -RRB- 10_1101-2021_01_07_425737 168 28 . . . 10_1101-2021_01_07_425737 169 1 ( ( -LRB- 10_1101-2021_01_07_425737 169 2 which which WDT 10_1101-2021_01_07_425737 169 3 was be VBD 10_1101-2021_01_07_425737 169 4 not not RB 10_1101-2021_01_07_425737 169 5 certified certify VBN 10_1101-2021_01_07_425737 169 6 by by IN 10_1101-2021_01_07_425737 169 7 peer peer NN 10_1101-2021_01_07_425737 169 8 review review NN 10_1101-2021_01_07_425737 169 9 ) ) -RRB- 10_1101-2021_01_07_425737 169 10 is be VBZ 10_1101-2021_01_07_425737 169 11 the the DT 10_1101-2021_01_07_425737 169 12 author author NN 10_1101-2021_01_07_425737 169 13 / / SYM 10_1101-2021_01_07_425737 169 14 funder funder NN 10_1101-2021_01_07_425737 169 15 . . . 10_1101-2021_01_07_425737 170 1 All all DT 10_1101-2021_01_07_425737 170 2 rights right NNS 10_1101-2021_01_07_425737 170 3 reserved reserve VBD 10_1101-2021_01_07_425737 170 4 . . . 10_1101-2021_01_07_425737 171 1 No no DT 10_1101-2021_01_07_425737 171 2 reuse reuse NN 10_1101-2021_01_07_425737 171 3 allowed allow VBN 10_1101-2021_01_07_425737 171 4 without without IN 10_1101-2021_01_07_425737 171 5 permission permission NN 10_1101-2021_01_07_425737 171 6 . . . 10_1101-2021_01_07_425737 172 1 The the DT 10_1101-2021_01_07_425737 172 2 copyright copyright NN 10_1101-2021_01_07_425737 172 3 holder holder NN 10_1101-2021_01_07_425737 172 4 for for IN 10_1101-2021_01_07_425737 172 5 this this DT 10_1101-2021_01_07_425737 172 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 172 7 version version NN 10_1101-2021_01_07_425737 172 8 posted post VBD 10_1101-2021_01_07_425737 172 9 January January NNP 10_1101-2021_01_07_425737 172 10 8 8 CD 10_1101-2021_01_07_425737 172 11 , , , 10_1101-2021_01_07_425737 172 12 2021 2021 CD 10_1101-2021_01_07_425737 172 13 . . . 10_1101-2021_01_07_425737 172 14 ; ; : 10_1101-2021_01_07_425737 172 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 172 16 : : : 10_1101-2021_01_07_425737 172 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 172 18 preprint preprint NN 10_1101-2021_01_07_425737 172 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 172 20 Supplementary Supplementary NNP 10_1101-2021_01_07_425737 172 21 Figure Figure NNP 10_1101-2021_01_07_425737 172 22 2 2 CD 10_1101-2021_01_07_425737 172 23 : : : 10_1101-2021_01_07_425737 172 24 Dotplot Dotplot NNP 10_1101-2021_01_07_425737 172 25 of of IN 10_1101-2021_01_07_425737 172 26 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 172 27 aphidicola aphidicola NNP 10_1101-2021_01_07_425737 172 28 flagellum flagellum NN 10_1101-2021_01_07_425737 172 29 proteins protein NNS 10_1101-2021_01_07_425737 172 30 found find VBN 10_1101-2021_01_07_425737 172 31 after after IN 10_1101-2021_01_07_425737 172 32 LC LC NNP 10_1101-2021_01_07_425737 172 33 / / SYM 10_1101-2021_01_07_425737 172 34 MS- MS- NNP 10_1101-2021_01_07_425737 172 35 MS MS NNP 10_1101-2021_01_07_425737 172 36 analysis analysis NN 10_1101-2021_01_07_425737 172 37 . . . 10_1101-2021_01_07_425737 173 1 The the DT 10_1101-2021_01_07_425737 173 2 enrichment enrichment NN 10_1101-2021_01_07_425737 173 3 score score NN 10_1101-2021_01_07_425737 173 4 for for IN 10_1101-2021_01_07_425737 173 5 each each DT 10_1101-2021_01_07_425737 173 6 protein protein NN 10_1101-2021_01_07_425737 173 7 is be VBZ 10_1101-2021_01_07_425737 173 8 indicated indicate VBN 10_1101-2021_01_07_425737 173 9 on on IN 10_1101-2021_01_07_425737 173 10 the the DT 10_1101-2021_01_07_425737 173 11 x x NN 10_1101-2021_01_07_425737 173 12 axis axis NN 10_1101-2021_01_07_425737 173 13 . . . 10_1101-2021_01_07_425737 174 1 Enrichment enrichment NN 10_1101-2021_01_07_425737 174 2 scores score NNS 10_1101-2021_01_07_425737 174 3 are be VBP 10_1101-2021_01_07_425737 174 4 calculated calculate VBN 10_1101-2021_01_07_425737 174 5 by by IN 10_1101-2021_01_07_425737 174 6 dividing divide VBG 10_1101-2021_01_07_425737 174 7 unique unique JJ 10_1101-2021_01_07_425737 174 8 spectral spectral NN 10_1101-2021_01_07_425737 174 9 counts count NNS 10_1101-2021_01_07_425737 174 10 for for IN 10_1101-2021_01_07_425737 174 11 each each DT 10_1101-2021_01_07_425737 174 12 protein protein NN 10_1101-2021_01_07_425737 174 13 in in IN 10_1101-2021_01_07_425737 174 14 the the DT 10_1101-2021_01_07_425737 174 15 final final JJ 10_1101-2021_01_07_425737 174 16 step step NN 10_1101-2021_01_07_425737 174 17 by by IN 10_1101-2021_01_07_425737 174 18 each each DT 10_1101-2021_01_07_425737 174 19 protein protein NN 10_1101-2021_01_07_425737 174 20 present present JJ 10_1101-2021_01_07_425737 174 21 in in IN 10_1101-2021_01_07_425737 174 22 the the DT 10_1101-2021_01_07_425737 174 23 cell cell NN 10_1101-2021_01_07_425737 174 24 lysate lysate NN 10_1101-2021_01_07_425737 174 25 . . . 10_1101-2021_01_07_425737 175 1 Core core NN 10_1101-2021_01_07_425737 175 2 flagellum flagellum NN 10_1101-2021_01_07_425737 175 3 proteins protein NNS 10_1101-2021_01_07_425737 175 4 ( ( -LRB- 10_1101-2021_01_07_425737 175 5 defined define VBN 10_1101-2021_01_07_425737 175 6 by by IN 10_1101-2021_01_07_425737 175 7 proteins protein NNS 10_1101-2021_01_07_425737 175 8 required require VBN 10_1101-2021_01_07_425737 175 9 for for IN 10_1101-2021_01_07_425737 175 10 type type NN 10_1101-2021_01_07_425737 175 11 III iii CD 10_1101-2021_01_07_425737 175 12 secretion secretion NN 10_1101-2021_01_07_425737 175 13 activity activity NN 10_1101-2021_01_07_425737 175 14 and and CC 10_1101-2021_01_07_425737 175 15 flagellum flagellum NN 10_1101-2021_01_07_425737 175 16 structure structure NN 10_1101-2021_01_07_425737 175 17 ) ) -RRB- 10_1101-2021_01_07_425737 175 18 are be VBP 10_1101-2021_01_07_425737 175 19 filled fill VBN 10_1101-2021_01_07_425737 175 20 in in IN 10_1101-2021_01_07_425737 175 21 green green JJ 10_1101-2021_01_07_425737 175 22 , , , 10_1101-2021_01_07_425737 175 23 accessory accessory JJ 10_1101-2021_01_07_425737 175 24 proteins protein NNS 10_1101-2021_01_07_425737 175 25 are be VBP 10_1101-2021_01_07_425737 175 26 filled fill VBN 10_1101-2021_01_07_425737 175 27 in in IN 10_1101-2021_01_07_425737 175 28 white white NNP 10_1101-2021_01_07_425737 175 29 . . . 10_1101-2021_01_07_425737 176 1 flgB flgb CD 10_1101-2021_01_07_425737 176 2 flgK flgk CD 10_1101-2021_01_07_425737 176 3 fliE flie CD 10_1101-2021_01_07_425737 176 4 fliN flin JJ 10_1101-2021_01_07_425737 176 5 fliP flip CD 10_1101-2021_01_07_425737 176 6 fliI fliI NNP 10_1101-2021_01_07_425737 176 7 fliG flig CD 10_1101-2021_01_07_425737 176 8 flgC flgc CD 10_1101-2021_01_07_425737 176 9 flhA flha CD 10_1101-2021_01_07_425737 176 10 fliM flim CD 10_1101-2021_01_07_425737 176 11 fliH flih CD 10_1101-2021_01_07_425737 176 12 flgE flge CD 10_1101-2021_01_07_425737 176 13 flgI flgi CD 10_1101-2021_01_07_425737 176 14 fliF flif CD 10_1101-2021_01_07_425737 176 15 flgH flgh CD 10_1101-2021_01_07_425737 176 16 flgF flgf CD 10_1101-2021_01_07_425737 176 17 flgG flgg CD 10_1101-2021_01_07_425737 176 18 0 0 CD 10_1101-2021_01_07_425737 176 19 10 10 CD 10_1101-2021_01_07_425737 176 20 20 20 CD 10_1101-2021_01_07_425737 176 21 30 30 CD 10_1101-2021_01_07_425737 176 22 Enrichment enrichment NN 10_1101-2021_01_07_425737 176 23 score score NN 10_1101-2021_01_07_425737 176 24 after after IN 10_1101-2021_01_07_425737 176 25 isolation isolation NN 10_1101-2021_01_07_425737 176 26 procedure procedure NN 10_1101-2021_01_07_425737 176 27 P p NN 10_1101-2021_01_07_425737 176 28 ro ro NN 10_1101-2021_01_07_425737 176 29 te te NNP 10_1101-2021_01_07_425737 176 30 in in IN 10_1101-2021_01_07_425737 176 31 type type NN 10_1101-2021_01_07_425737 176 32 accessory accessory NN 10_1101-2021_01_07_425737 176 33 core core NN 10_1101-2021_01_07_425737 176 34 ( ( -LRB- 10_1101-2021_01_07_425737 176 35 which which WDT 10_1101-2021_01_07_425737 176 36 was be VBD 10_1101-2021_01_07_425737 176 37 not not RB 10_1101-2021_01_07_425737 176 38 certified certify VBN 10_1101-2021_01_07_425737 176 39 by by IN 10_1101-2021_01_07_425737 176 40 peer peer NN 10_1101-2021_01_07_425737 176 41 review review NN 10_1101-2021_01_07_425737 176 42 ) ) -RRB- 10_1101-2021_01_07_425737 176 43 is be VBZ 10_1101-2021_01_07_425737 176 44 the the DT 10_1101-2021_01_07_425737 176 45 author author NN 10_1101-2021_01_07_425737 176 46 / / SYM 10_1101-2021_01_07_425737 176 47 funder funder NN 10_1101-2021_01_07_425737 176 48 . . . 10_1101-2021_01_07_425737 177 1 All all DT 10_1101-2021_01_07_425737 177 2 rights right NNS 10_1101-2021_01_07_425737 177 3 reserved reserve VBD 10_1101-2021_01_07_425737 177 4 . . . 10_1101-2021_01_07_425737 178 1 No no DT 10_1101-2021_01_07_425737 178 2 reuse reuse NN 10_1101-2021_01_07_425737 178 3 allowed allow VBN 10_1101-2021_01_07_425737 178 4 without without IN 10_1101-2021_01_07_425737 178 5 permission permission NN 10_1101-2021_01_07_425737 178 6 . . . 10_1101-2021_01_07_425737 179 1 The the DT 10_1101-2021_01_07_425737 179 2 copyright copyright NN 10_1101-2021_01_07_425737 179 3 holder holder NN 10_1101-2021_01_07_425737 179 4 for for IN 10_1101-2021_01_07_425737 179 5 this this DT 10_1101-2021_01_07_425737 179 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 179 7 version version NN 10_1101-2021_01_07_425737 179 8 posted post VBD 10_1101-2021_01_07_425737 179 9 January January NNP 10_1101-2021_01_07_425737 179 10 8 8 CD 10_1101-2021_01_07_425737 179 11 , , , 10_1101-2021_01_07_425737 179 12 2021 2021 CD 10_1101-2021_01_07_425737 179 13 . . . 10_1101-2021_01_07_425737 179 14 ; ; : 10_1101-2021_01_07_425737 179 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 179 16 : : : 10_1101-2021_01_07_425737 179 17 bioRxiv biorxiv VB 10_1101-2021_01_07_425737 179 18 preprint preprint NN 10_1101-2021_01_07_425737 179 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 179 20 References References NNP 10_1101-2021_01_07_425737 179 21 1 1 CD 10_1101-2021_01_07_425737 179 22 . . . 10_1101-2021_01_07_425737 180 1 Munson Munson NNP 10_1101-2021_01_07_425737 180 2 , , , 10_1101-2021_01_07_425737 180 3 M.A. M.A. NNP 10_1101-2021_01_07_425737 180 4 , , , 10_1101-2021_01_07_425737 180 5 Baumann Baumann NNP 10_1101-2021_01_07_425737 180 6 , , , 10_1101-2021_01_07_425737 180 7 P. P. NNP 10_1101-2021_01_07_425737 180 8 , , , 10_1101-2021_01_07_425737 180 9 Kinsey Kinsey NNP 10_1101-2021_01_07_425737 180 10 , , , 10_1101-2021_01_07_425737 180 11 M.G. M.G. NNP 10_1101-2021_01_07_425737 181 1 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 181 2 gen gen NNP 10_1101-2021_01_07_425737 181 3 . . NNP 10_1101-2021_01_07_425737 181 4 nov nov NNP 10_1101-2021_01_07_425737 181 5 . . NNP 10_1101-2021_01_07_425737 181 6 and and CC 10_1101-2021_01_07_425737 181 7 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 181 8 aphidicola aphidicola NNP 10_1101-2021_01_07_425737 181 9 sp sp NN 10_1101-2021_01_07_425737 181 10 . . . 10_1101-2021_01_07_425737 182 1 nov nov NNP 10_1101-2021_01_07_425737 182 2 . . NNP 10_1101-2021_01_07_425737 182 3 , , , 10_1101-2021_01_07_425737 182 4 a a DT 10_1101-2021_01_07_425737 182 5 taxon taxon NN 10_1101-2021_01_07_425737 182 6 consisting consist VBG 10_1101-2021_01_07_425737 182 7 of of IN 10_1101-2021_01_07_425737 182 8 the the DT 10_1101-2021_01_07_425737 182 9 mycetocyte mycetocyte NN 10_1101-2021_01_07_425737 182 10 - - HYPH 10_1101-2021_01_07_425737 182 11 associated associate VBN 10_1101-2021_01_07_425737 182 12 , , , 10_1101-2021_01_07_425737 182 13 primary primary JJ 10_1101-2021_01_07_425737 182 14 endosymbionts endosymbiont NNS 10_1101-2021_01_07_425737 182 15 of of IN 10_1101-2021_01_07_425737 182 16 aphids aphid NNS 10_1101-2021_01_07_425737 182 17 . . . 10_1101-2021_01_07_425737 183 1 Int Int NNP 10_1101-2021_01_07_425737 183 2 . . . 10_1101-2021_01_07_425737 184 1 J. J. NNP 10_1101-2021_01_07_425737 184 2 Syst Syst NNP 10_1101-2021_01_07_425737 184 3 . . . 10_1101-2021_01_07_425737 185 1 Bacteriol Bacteriol NNP 10_1101-2021_01_07_425737 185 2 . . . 10_1101-2021_01_07_425737 186 1 1991 1991 CD 10_1101-2021_01_07_425737 186 2 ; ; : 10_1101-2021_01_07_425737 186 3 41:566 41:566 CD 10_1101-2021_01_07_425737 186 4 - - HYPH 10_1101-2021_01_07_425737 186 5 568 568 CD 10_1101-2021_01_07_425737 186 6 2 2 CD 10_1101-2021_01_07_425737 186 7 . . . 10_1101-2021_01_07_425737 187 1 Shigenobu Shigenobu NNP 10_1101-2021_01_07_425737 187 2 , , , 10_1101-2021_01_07_425737 187 3 S. S. NNP 10_1101-2021_01_07_425737 187 4 , , , 10_1101-2021_01_07_425737 187 5 Watanabe Watanabe NNP 10_1101-2021_01_07_425737 187 6 , , , 10_1101-2021_01_07_425737 187 7 H. H. NNP 10_1101-2021_01_07_425737 187 8 , , , 10_1101-2021_01_07_425737 187 9 Hattori Hattori NNP 10_1101-2021_01_07_425737 187 10 , , , 10_1101-2021_01_07_425737 187 11 M. M. NNP 10_1101-2021_01_07_425737 187 12 , , , 10_1101-2021_01_07_425737 187 13 Sakaki Sakaki NNP 10_1101-2021_01_07_425737 187 14 , , , 10_1101-2021_01_07_425737 187 15 Y. Y. NNP 10_1101-2021_01_07_425737 188 1 & & CC 10_1101-2021_01_07_425737 188 2 Ishikawa Ishikawa NNP 10_1101-2021_01_07_425737 188 3 , , , 10_1101-2021_01_07_425737 188 4 H. H. NNP 10_1101-2021_01_07_425737 188 5 Genome Genome NNP 10_1101-2021_01_07_425737 188 6 sequence sequence NN 10_1101-2021_01_07_425737 188 7 of of IN 10_1101-2021_01_07_425737 188 8 the the DT 10_1101-2021_01_07_425737 188 9 endocellular endocellular JJ 10_1101-2021_01_07_425737 188 10 bacterial bacterial JJ 10_1101-2021_01_07_425737 188 11 symbiont symbiont NN 10_1101-2021_01_07_425737 188 12 of of IN 10_1101-2021_01_07_425737 188 13 aphids aphids NNP 10_1101-2021_01_07_425737 188 14 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 188 15 sp sp NNP 10_1101-2021_01_07_425737 188 16 . . . 10_1101-2021_01_07_425737 189 1 APS APS NNP 10_1101-2021_01_07_425737 189 2 . . . 10_1101-2021_01_07_425737 190 1 Nature nature NN 10_1101-2021_01_07_425737 190 2 407 407 CD 10_1101-2021_01_07_425737 190 3 , , , 10_1101-2021_01_07_425737 190 4 81 81 CD 10_1101-2021_01_07_425737 190 5 - - SYM 10_1101-2021_01_07_425737 190 6 86 86 CD 10_1101-2021_01_07_425737 190 7 ( ( -LRB- 10_1101-2021_01_07_425737 190 8 2000 2000 CD 10_1101-2021_01_07_425737 190 9 ) ) -RRB- 10_1101-2021_01_07_425737 190 10 . . . 10_1101-2021_01_07_425737 191 1 3 3 LS 10_1101-2021_01_07_425737 191 2 . . . 10_1101-2021_01_07_425737 192 1 Moran Moran NNP 10_1101-2021_01_07_425737 192 2 , , , 10_1101-2021_01_07_425737 192 3 N. N. NNP 10_1101-2021_01_07_425737 192 4 A. a. NN 10_1101-2021_01_07_425737 193 1 & & CC 10_1101-2021_01_07_425737 193 2 Bennett Bennett NNP 10_1101-2021_01_07_425737 193 3 , , , 10_1101-2021_01_07_425737 193 4 G. G. NNP 10_1101-2021_01_07_425737 193 5 M. M. NNP 10_1101-2021_01_07_425737 193 6 The the DT 10_1101-2021_01_07_425737 193 7 tiniest tiny JJS 10_1101-2021_01_07_425737 193 8 tiny tiny JJ 10_1101-2021_01_07_425737 193 9 genomes genome NNS 10_1101-2021_01_07_425737 193 10 . . . 10_1101-2021_01_07_425737 194 1 Annu Annu NNP 10_1101-2021_01_07_425737 194 2 Rev Rev NNP 10_1101-2021_01_07_425737 194 3 Microbiol Microbiol NNP 10_1101-2021_01_07_425737 194 4 68 68 CD 10_1101-2021_01_07_425737 194 5 , , , 10_1101-2021_01_07_425737 194 6 195 195 CD 10_1101-2021_01_07_425737 194 7 - - SYM 10_1101-2021_01_07_425737 194 8 215 215 CD 10_1101-2021_01_07_425737 194 9 ( ( -LRB- 10_1101-2021_01_07_425737 194 10 2014 2014 CD 10_1101-2021_01_07_425737 194 11 ) ) -RRB- 10_1101-2021_01_07_425737 194 12 . . . 10_1101-2021_01_07_425737 195 1 4 4 LS 10_1101-2021_01_07_425737 195 2 . . . 10_1101-2021_01_07_425737 196 1 Tamas Tamas NNP 10_1101-2021_01_07_425737 196 2 , , , 10_1101-2021_01_07_425737 196 3 I. I. NNP 10_1101-2021_01_07_425737 196 4 et et NNP 10_1101-2021_01_07_425737 196 5 al al NNP 10_1101-2021_01_07_425737 196 6 . . . 10_1101-2021_01_07_425737 197 1 50 50 CD 10_1101-2021_01_07_425737 197 2 million million CD 10_1101-2021_01_07_425737 197 3 years year NNS 10_1101-2021_01_07_425737 197 4 of of IN 10_1101-2021_01_07_425737 197 5 genomic genomic JJ 10_1101-2021_01_07_425737 197 6 stasis stasis NN 10_1101-2021_01_07_425737 197 7 in in IN 10_1101-2021_01_07_425737 197 8 endosymbiotic endosymbiotic JJ 10_1101-2021_01_07_425737 197 9 bacteria bacteria NNS 10_1101-2021_01_07_425737 197 10 . . . 10_1101-2021_01_07_425737 198 1 Science science NN 10_1101-2021_01_07_425737 198 2 296 296 CD 10_1101-2021_01_07_425737 198 3 , , , 10_1101-2021_01_07_425737 198 4 2376 2376 CD 10_1101-2021_01_07_425737 198 5 - - SYM 10_1101-2021_01_07_425737 198 6 2379 2379 CD 10_1101-2021_01_07_425737 198 7 ( ( -LRB- 10_1101-2021_01_07_425737 198 8 2002 2002 CD 10_1101-2021_01_07_425737 198 9 ) ) -RRB- 10_1101-2021_01_07_425737 198 10 . . . 10_1101-2021_01_07_425737 199 1 5 5 CD 10_1101-2021_01_07_425737 199 2 . . . 10_1101-2021_01_07_425737 200 1 Wernegreen Wernegreen NNP 10_1101-2021_01_07_425737 200 2 , , , 10_1101-2021_01_07_425737 200 3 J. J. NNP 10_1101-2021_01_07_425737 200 4 J. J. NNP 10_1101-2021_01_07_425737 200 5 Genome Genome NNP 10_1101-2021_01_07_425737 200 6 evolution evolution NN 10_1101-2021_01_07_425737 200 7 in in IN 10_1101-2021_01_07_425737 200 8 bacterial bacterial JJ 10_1101-2021_01_07_425737 200 9 endosymbionts endosymbiont NNS 10_1101-2021_01_07_425737 200 10 of of IN 10_1101-2021_01_07_425737 200 11 insects insect NNS 10_1101-2021_01_07_425737 200 12 . . . 10_1101-2021_01_07_425737 201 1 Nature Nature NNP 10_1101-2021_01_07_425737 201 2 Reviews Reviews NNPS 10_1101-2021_01_07_425737 201 3 Genetics Genetics NNP 10_1101-2021_01_07_425737 201 4 3 3 CD 10_1101-2021_01_07_425737 201 5 , , , 10_1101-2021_01_07_425737 201 6 850 850 CD 10_1101-2021_01_07_425737 201 7 - - SYM 10_1101-2021_01_07_425737 201 8 861 861 CD 10_1101-2021_01_07_425737 201 9 ( ( -LRB- 10_1101-2021_01_07_425737 201 10 2002 2002 CD 10_1101-2021_01_07_425737 201 11 ) ) -RRB- 10_1101-2021_01_07_425737 201 12 . . . 10_1101-2021_01_07_425737 202 1 6 6 CD 10_1101-2021_01_07_425737 202 2 . . . 10_1101-2021_01_07_425737 203 1 Douglas Douglas NNP 10_1101-2021_01_07_425737 203 2 , , , 10_1101-2021_01_07_425737 203 3 A. a. NN 10_1101-2021_01_07_425737 203 4 E. E. NNP 10_1101-2021_01_07_425737 203 5 Nutritional Nutritional NNP 10_1101-2021_01_07_425737 203 6 interactions interaction NNS 10_1101-2021_01_07_425737 203 7 in in IN 10_1101-2021_01_07_425737 203 8 insect insect JJ 10_1101-2021_01_07_425737 203 9 - - HYPH 10_1101-2021_01_07_425737 203 10 microbial microbial NN 10_1101-2021_01_07_425737 203 11 symbioses symbiose NNS 10_1101-2021_01_07_425737 203 12 : : : 10_1101-2021_01_07_425737 203 13 aphids aphid NNS 10_1101-2021_01_07_425737 203 14 and and CC 10_1101-2021_01_07_425737 203 15 their -PRON- PRP$ 10_1101-2021_01_07_425737 203 16 symbiotic symbiotic JJ 10_1101-2021_01_07_425737 203 17 bacteria bacteria NNS 10_1101-2021_01_07_425737 203 18 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 203 19 . . . 10_1101-2021_01_07_425737 204 1 Annu Annu NNP 10_1101-2021_01_07_425737 204 2 Rev Rev NNP 10_1101-2021_01_07_425737 204 3 Entomol Entomol NNP 10_1101-2021_01_07_425737 204 4 43 43 CD 10_1101-2021_01_07_425737 204 5 , , , 10_1101-2021_01_07_425737 204 6 17 17 CD 10_1101-2021_01_07_425737 204 7 - - SYM 10_1101-2021_01_07_425737 204 8 37 37 CD 10_1101-2021_01_07_425737 204 9 ( ( -LRB- 10_1101-2021_01_07_425737 204 10 1998 1998 CD 10_1101-2021_01_07_425737 204 11 ) ) -RRB- 10_1101-2021_01_07_425737 204 12 . . . 10_1101-2021_01_07_425737 205 1 7 7 LS 10_1101-2021_01_07_425737 205 2 . . . 10_1101-2021_01_07_425737 206 1 Akman Akman NNP 10_1101-2021_01_07_425737 206 2 Gündüz Gündüz NNP 10_1101-2021_01_07_425737 206 3 , , , 10_1101-2021_01_07_425737 206 4 E. E. NNP 10_1101-2021_01_07_425737 206 5 & & CC 10_1101-2021_01_07_425737 206 6 Douglas Douglas NNP 10_1101-2021_01_07_425737 206 7 , , , 10_1101-2021_01_07_425737 206 8 A. a. NN 10_1101-2021_01_07_425737 206 9 E. E. NNP 10_1101-2021_01_07_425737 206 10 Symbiotic Symbiotic NNP 10_1101-2021_01_07_425737 206 11 bacteria bacteria NNS 10_1101-2021_01_07_425737 206 12 enable enable VBP 10_1101-2021_01_07_425737 206 13 insect insect NN 10_1101-2021_01_07_425737 206 14 to to TO 10_1101-2021_01_07_425737 206 15 use use VB 10_1101-2021_01_07_425737 206 16 a a DT 10_1101-2021_01_07_425737 206 17 nutritionally nutritionally RB 10_1101-2021_01_07_425737 206 18 inadequate inadequate JJ 10_1101-2021_01_07_425737 206 19 diet diet NN 10_1101-2021_01_07_425737 206 20 . . . 10_1101-2021_01_07_425737 207 1 Proc Proc NNP 10_1101-2021_01_07_425737 207 2 Biol Biol NNP 10_1101-2021_01_07_425737 207 3 Sci Sci NNP 10_1101-2021_01_07_425737 207 4 276 276 CD 10_1101-2021_01_07_425737 207 5 , , , 10_1101-2021_01_07_425737 207 6 987 987 CD 10_1101-2021_01_07_425737 207 7 - - SYM 10_1101-2021_01_07_425737 207 8 991 991 CD 10_1101-2021_01_07_425737 207 9 ( ( -LRB- 10_1101-2021_01_07_425737 207 10 2009 2009 CD 10_1101-2021_01_07_425737 207 11 ) ) -RRB- 10_1101-2021_01_07_425737 207 12 . . . 10_1101-2021_01_07_425737 208 1 8 8 LS 10_1101-2021_01_07_425737 208 2 . . . 10_1101-2021_01_07_425737 209 1 Nakabachi Nakabachi NNP 10_1101-2021_01_07_425737 209 2 , , , 10_1101-2021_01_07_425737 209 3 A. a. NN 10_1101-2021_01_07_425737 210 1 & & CC 10_1101-2021_01_07_425737 210 2 Ishikawa Ishikawa NNP 10_1101-2021_01_07_425737 210 3 , , , 10_1101-2021_01_07_425737 210 4 H. H. NNP 10_1101-2021_01_07_425737 210 5 Provision Provision NNP 10_1101-2021_01_07_425737 210 6 of of IN 10_1101-2021_01_07_425737 210 7 riboflavin riboflavin RB 10_1101-2021_01_07_425737 210 8 to to IN 10_1101-2021_01_07_425737 210 9 the the DT 10_1101-2021_01_07_425737 210 10 host host NN 10_1101-2021_01_07_425737 210 11 aphid aphid NN 10_1101-2021_01_07_425737 210 12 , , , 10_1101-2021_01_07_425737 210 13 Acyrthosiphon Acyrthosiphon NNP 10_1101-2021_01_07_425737 210 14 pisum pisum NN 10_1101-2021_01_07_425737 210 15 , , , 10_1101-2021_01_07_425737 210 16 by by IN 10_1101-2021_01_07_425737 210 17 endosymbiotic endosymbiotic JJ 10_1101-2021_01_07_425737 210 18 bacteria bacteria NNS 10_1101-2021_01_07_425737 210 19 , , , 10_1101-2021_01_07_425737 210 20 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 210 21 . . . 10_1101-2021_01_07_425737 211 1 J J NNP 10_1101-2021_01_07_425737 211 2 Insect Insect NNP 10_1101-2021_01_07_425737 211 3 Physiol Physiol NNP 10_1101-2021_01_07_425737 211 4 45 45 CD 10_1101-2021_01_07_425737 211 5 , , , 10_1101-2021_01_07_425737 211 6 1 1 CD 10_1101-2021_01_07_425737 211 7 - - SYM 10_1101-2021_01_07_425737 211 8 6 6 CD 10_1101-2021_01_07_425737 211 9 ( ( -LRB- 10_1101-2021_01_07_425737 211 10 1999 1999 CD 10_1101-2021_01_07_425737 211 11 ) ) -RRB- 10_1101-2021_01_07_425737 211 12 . . . 10_1101-2021_01_07_425737 212 1 9 9 CD 10_1101-2021_01_07_425737 212 2 . . . 10_1101-2021_01_07_425737 213 1 Charles Charles NNP 10_1101-2021_01_07_425737 213 2 , , , 10_1101-2021_01_07_425737 213 3 H. H. NNP 10_1101-2021_01_07_425737 213 4 , , , 10_1101-2021_01_07_425737 213 5 Calevro Calevro NNP 10_1101-2021_01_07_425737 213 6 , , , 10_1101-2021_01_07_425737 213 7 F. F. NNP 10_1101-2021_01_07_425737 213 8 , , , 10_1101-2021_01_07_425737 213 9 Vinuelas Vinuelas NNP 10_1101-2021_01_07_425737 213 10 , , , 10_1101-2021_01_07_425737 213 11 J. J. NNP 10_1101-2021_01_07_425737 213 12 , , , 10_1101-2021_01_07_425737 213 13 Fayard Fayard NNP 10_1101-2021_01_07_425737 213 14 , , , 10_1101-2021_01_07_425737 213 15 J. J. NNP 10_1101-2021_01_07_425737 213 16 M. M. NNP 10_1101-2021_01_07_425737 213 17 & & CC 10_1101-2021_01_07_425737 213 18 Rahbe Rahbe NNP 10_1101-2021_01_07_425737 213 19 , , , 10_1101-2021_01_07_425737 213 20 Y. Y. NNP 10_1101-2021_01_07_425737 213 21 Codon Codon NNP 10_1101-2021_01_07_425737 213 22 usage usage NN 10_1101-2021_01_07_425737 213 23 bias bias NN 10_1101-2021_01_07_425737 213 24 and and CC 10_1101-2021_01_07_425737 213 25 tRNA trna NN 10_1101-2021_01_07_425737 213 26 over over IN 10_1101-2021_01_07_425737 213 27 - - HYPH 10_1101-2021_01_07_425737 213 28 expression expression NN 10_1101-2021_01_07_425737 213 29 in in IN 10_1101-2021_01_07_425737 213 30 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 213 31 aphidicola aphidicola NN 10_1101-2021_01_07_425737 213 32 after after IN 10_1101-2021_01_07_425737 213 33 aromatic aromatic NNP 10_1101-2021_01_07_425737 213 34 amino amino NNP 10_1101-2021_01_07_425737 213 35 acid acid NNP 10_1101-2021_01_07_425737 213 36 nutritional nutritional JJ 10_1101-2021_01_07_425737 213 37 stress stress NN 10_1101-2021_01_07_425737 213 38 on on IN 10_1101-2021_01_07_425737 213 39 its -PRON- PRP$ 10_1101-2021_01_07_425737 213 40 host host NN 10_1101-2021_01_07_425737 213 41 Acyrthosiphon Acyrthosiphon NNP 10_1101-2021_01_07_425737 213 42 pisum pisum NN 10_1101-2021_01_07_425737 213 43 . . . 10_1101-2021_01_07_425737 214 1 Nucleic Nucleic NNP 10_1101-2021_01_07_425737 214 2 Acids Acids NNPS 10_1101-2021_01_07_425737 214 3 Res Res NNP 10_1101-2021_01_07_425737 214 4 34 34 CD 10_1101-2021_01_07_425737 214 5 , , , 10_1101-2021_01_07_425737 214 6 4583 4583 CD 10_1101-2021_01_07_425737 214 7 - - SYM 10_1101-2021_01_07_425737 214 8 4592 4592 CD 10_1101-2021_01_07_425737 214 9 ( ( -LRB- 10_1101-2021_01_07_425737 214 10 2006 2006 CD 10_1101-2021_01_07_425737 214 11 ) ) -RRB- 10_1101-2021_01_07_425737 214 12 . . . 10_1101-2021_01_07_425737 215 1 10 10 CD 10_1101-2021_01_07_425737 215 2 . . . 10_1101-2021_01_07_425737 216 1 Charles Charles NNP 10_1101-2021_01_07_425737 216 2 , , , 10_1101-2021_01_07_425737 216 3 H. H. NNP 10_1101-2021_01_07_425737 216 4 et et NNP 10_1101-2021_01_07_425737 216 5 al al NNP 10_1101-2021_01_07_425737 216 6 . . . 10_1101-2021_01_07_425737 217 1 A a DT 10_1101-2021_01_07_425737 217 2 genomic genomic JJ 10_1101-2021_01_07_425737 217 3 reappraisal reappraisal NN 10_1101-2021_01_07_425737 217 4 of of IN 10_1101-2021_01_07_425737 217 5 symbiotic symbiotic JJ 10_1101-2021_01_07_425737 217 6 function function NN 10_1101-2021_01_07_425737 217 7 in in IN 10_1101-2021_01_07_425737 217 8 the the DT 10_1101-2021_01_07_425737 217 9 aphid aphid NNP 10_1101-2021_01_07_425737 217 10 / / SYM 10_1101-2021_01_07_425737 217 11 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 217 12 symbiosis symbiosis NN 10_1101-2021_01_07_425737 217 13 : : : 10_1101-2021_01_07_425737 217 14 reduced reduce VBN 10_1101-2021_01_07_425737 217 15 transporter transporter NN 10_1101-2021_01_07_425737 217 16 sets set NNS 10_1101-2021_01_07_425737 217 17 and and CC 10_1101-2021_01_07_425737 217 18 variable variable JJ 10_1101-2021_01_07_425737 217 19 membrane membrane NN 10_1101-2021_01_07_425737 217 20 organisations organisation NNS 10_1101-2021_01_07_425737 217 21 . . . 10_1101-2021_01_07_425737 218 1 PLoS plos ADD 10_1101-2021_01_07_425737 218 2 One one CD 10_1101-2021_01_07_425737 218 3 6 6 CD 10_1101-2021_01_07_425737 218 4 , , , 10_1101-2021_01_07_425737 218 5 e29096 e29096 NNP 10_1101-2021_01_07_425737 218 6 ( ( -LRB- 10_1101-2021_01_07_425737 218 7 2011 2011 CD 10_1101-2021_01_07_425737 218 8 ) ) -RRB- 10_1101-2021_01_07_425737 218 9 . . . 10_1101-2021_01_07_425737 219 1 11 11 CD 10_1101-2021_01_07_425737 219 2 . . . 10_1101-2021_01_07_425737 220 1 Poliakov Poliakov NNP 10_1101-2021_01_07_425737 220 2 , , , 10_1101-2021_01_07_425737 220 3 A. A. NNP 10_1101-2021_01_07_425737 220 4 et et FW 10_1101-2021_01_07_425737 220 5 al al NNP 10_1101-2021_01_07_425737 220 6 . . . 10_1101-2021_01_07_425737 221 1 Large large JJ 10_1101-2021_01_07_425737 221 2 - - HYPH 10_1101-2021_01_07_425737 221 3 scale scale NN 10_1101-2021_01_07_425737 221 4 label label NN 10_1101-2021_01_07_425737 221 5 - - HYPH 10_1101-2021_01_07_425737 221 6 free free JJ 10_1101-2021_01_07_425737 221 7 quantitative quantitative JJ 10_1101-2021_01_07_425737 221 8 proteomics proteomic NNS 10_1101-2021_01_07_425737 221 9 of of IN 10_1101-2021_01_07_425737 221 10 the the DT 10_1101-2021_01_07_425737 221 11 pea pea NN 10_1101-2021_01_07_425737 221 12 aphid- aphid- NNP 10_1101-2021_01_07_425737 221 13 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 221 14 symbiosis symbiosis NN 10_1101-2021_01_07_425737 221 15 . . . 10_1101-2021_01_07_425737 222 1 Mol Mol NNP 10_1101-2021_01_07_425737 222 2 Cell Cell NNP 10_1101-2021_01_07_425737 222 3 Proteomics Proteomics NNP 10_1101-2021_01_07_425737 222 4 10 10 CD 10_1101-2021_01_07_425737 222 5 , , , 10_1101-2021_01_07_425737 222 6 M110.007039 M110.007039 NFP 10_1101-2021_01_07_425737 222 7 ( ( -LRB- 10_1101-2021_01_07_425737 222 8 2011 2011 CD 10_1101-2021_01_07_425737 222 9 ) ) -RRB- 10_1101-2021_01_07_425737 222 10 . . . 10_1101-2021_01_07_425737 223 1 12 12 CD 10_1101-2021_01_07_425737 223 2 . . . 10_1101-2021_01_07_425737 224 1 Maezawa Maezawa NNP 10_1101-2021_01_07_425737 224 2 , , , 10_1101-2021_01_07_425737 224 3 K. K. NNP 10_1101-2021_01_07_425737 224 4 et et NNP 10_1101-2021_01_07_425737 224 5 al al NNP 10_1101-2021_01_07_425737 224 6 . . . 10_1101-2021_01_07_425737 225 1 Hundreds hundred NNS 10_1101-2021_01_07_425737 225 2 of of IN 10_1101-2021_01_07_425737 225 3 flagellar flagellar JJ 10_1101-2021_01_07_425737 225 4 basal basal NN 10_1101-2021_01_07_425737 225 5 bodies body NNS 10_1101-2021_01_07_425737 225 6 cover cover VBP 10_1101-2021_01_07_425737 225 7 the the DT 10_1101-2021_01_07_425737 225 8 cell cell NN 10_1101-2021_01_07_425737 225 9 surface surface NN 10_1101-2021_01_07_425737 225 10 of of IN 10_1101-2021_01_07_425737 225 11 the the DT 10_1101-2021_01_07_425737 225 12 endosymbiotic endosymbiotic JJ 10_1101-2021_01_07_425737 225 13 bacterium bacterium NN 10_1101-2021_01_07_425737 225 14 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 225 15 aphidicola aphidicola NNP 10_1101-2021_01_07_425737 225 16 sp sp NN 10_1101-2021_01_07_425737 225 17 . . . 10_1101-2021_01_07_425737 226 1 strain strain VB 10_1101-2021_01_07_425737 226 2 APS APS NNP 10_1101-2021_01_07_425737 226 3 . . . 10_1101-2021_01_07_425737 227 1 J J NNP 10_1101-2021_01_07_425737 227 2 Bacteriol Bacteriol NNP 10_1101-2021_01_07_425737 227 3 188 188 CD 10_1101-2021_01_07_425737 227 4 , , , 10_1101-2021_01_07_425737 227 5 6539 6539 CD 10_1101-2021_01_07_425737 227 6 - - SYM 10_1101-2021_01_07_425737 227 7 6543 6543 CD 10_1101-2021_01_07_425737 227 8 ( ( -LRB- 10_1101-2021_01_07_425737 227 9 2006 2006 CD 10_1101-2021_01_07_425737 227 10 ) ) -RRB- 10_1101-2021_01_07_425737 227 11 . . . 10_1101-2021_01_07_425737 228 1 13 13 CD 10_1101-2021_01_07_425737 228 2 . . . 10_1101-2021_01_07_425737 229 1 Denise Denise NNP 10_1101-2021_01_07_425737 229 2 , , , 10_1101-2021_01_07_425737 229 3 R. R. NNP 10_1101-2021_01_07_425737 229 4 , , , 10_1101-2021_01_07_425737 229 5 Abby Abby NNP 10_1101-2021_01_07_425737 229 6 , , , 10_1101-2021_01_07_425737 229 7 S. S. NNP 10_1101-2021_01_07_425737 229 8 S. S. NNP 10_1101-2021_01_07_425737 229 9 & & CC 10_1101-2021_01_07_425737 229 10 Rocha Rocha NNP 10_1101-2021_01_07_425737 229 11 , , , 10_1101-2021_01_07_425737 229 12 E. E. NNP 10_1101-2021_01_07_425737 229 13 P. P. NNP 10_1101-2021_01_07_425737 229 14 C. C. NNP 10_1101-2021_01_07_425737 229 15 The The NNP 10_1101-2021_01_07_425737 229 16 Evolution Evolution NNP 10_1101-2021_01_07_425737 229 17 of of IN 10_1101-2021_01_07_425737 229 18 Protein Protein NNP 10_1101-2021_01_07_425737 229 19 Secretion Secretion NNP 10_1101-2021_01_07_425737 229 20 Systems Systems NNPS 10_1101-2021_01_07_425737 229 21 by by IN 10_1101-2021_01_07_425737 229 22 Co co NN 10_1101-2021_01_07_425737 229 23 - - NN 10_1101-2021_01_07_425737 229 24 option option NN 10_1101-2021_01_07_425737 229 25 and and CC 10_1101-2021_01_07_425737 229 26 Tinkering Tinkering NNP 10_1101-2021_01_07_425737 229 27 of of IN 10_1101-2021_01_07_425737 229 28 Cellular Cellular NNP 10_1101-2021_01_07_425737 229 29 Machineries Machineries NNPS 10_1101-2021_01_07_425737 229 30 . . . 10_1101-2021_01_07_425737 230 1 Trends trend NNS 10_1101-2021_01_07_425737 230 2 in in IN 10_1101-2021_01_07_425737 230 3 Microbiology Microbiology NNP 10_1101-2021_01_07_425737 230 4 ( ( -LRB- 10_1101-2021_01_07_425737 230 5 2020 2020 CD 10_1101-2021_01_07_425737 230 6 ) ) -RRB- 10_1101-2021_01_07_425737 230 7 . . . 10_1101-2021_01_07_425737 231 1 14 14 CD 10_1101-2021_01_07_425737 231 2 . . . 10_1101-2021_01_07_425737 232 1 Chong Chong NNP 10_1101-2021_01_07_425737 232 2 , , , 10_1101-2021_01_07_425737 232 3 R. R. NNP 10_1101-2021_01_07_425737 232 4 A. A. NNP 10_1101-2021_01_07_425737 232 5 , , , 10_1101-2021_01_07_425737 232 6 Park Park NNP 10_1101-2021_01_07_425737 232 7 , , , 10_1101-2021_01_07_425737 232 8 H. H. NNP 10_1101-2021_01_07_425737 232 9 & & CC 10_1101-2021_01_07_425737 232 10 Moran Moran NNP 10_1101-2021_01_07_425737 232 11 , , , 10_1101-2021_01_07_425737 232 12 N. N. NNP 10_1101-2021_01_07_425737 232 13 A. a. NN 10_1101-2021_01_07_425737 232 14 Genome Genome NNP 10_1101-2021_01_07_425737 232 15 evolution evolution NN 10_1101-2021_01_07_425737 232 16 of of IN 10_1101-2021_01_07_425737 232 17 the the DT 10_1101-2021_01_07_425737 232 18 obligate obligate NN 10_1101-2021_01_07_425737 232 19 endosymbiont endosymbiont NNP 10_1101-2021_01_07_425737 232 20 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 232 21 aphidicola aphidicola NN 10_1101-2021_01_07_425737 232 22 . . . 10_1101-2021_01_07_425737 233 1 Mol Mol NNP 10_1101-2021_01_07_425737 233 2 Biol Biol NNP 10_1101-2021_01_07_425737 233 3 Evol Evol NNP 10_1101-2021_01_07_425737 233 4 ( ( -LRB- 10_1101-2021_01_07_425737 233 5 2019 2019 CD 10_1101-2021_01_07_425737 233 6 ) ) -RRB- 10_1101-2021_01_07_425737 233 7 . . . 10_1101-2021_01_07_425737 234 1 15 15 CD 10_1101-2021_01_07_425737 234 2 . . . 10_1101-2021_01_07_425737 235 1 Cornelis Cornelis NNP 10_1101-2021_01_07_425737 235 2 , , , 10_1101-2021_01_07_425737 235 3 G. G. NNP 10_1101-2021_01_07_425737 235 4 R. R. NNP 10_1101-2021_01_07_425737 235 5 & & CC 10_1101-2021_01_07_425737 235 6 Van Van NNP 10_1101-2021_01_07_425737 235 7 Gijsegem Gijsegem NNP 10_1101-2021_01_07_425737 235 8 , , , 10_1101-2021_01_07_425737 235 9 F. F. NNP 10_1101-2021_01_07_425737 235 10 Assembly Assembly NNP 10_1101-2021_01_07_425737 235 11 and and CC 10_1101-2021_01_07_425737 235 12 function function NN 10_1101-2021_01_07_425737 235 13 of of IN 10_1101-2021_01_07_425737 235 14 type type NN 10_1101-2021_01_07_425737 235 15 III III NNP 10_1101-2021_01_07_425737 235 16 secretory secretory NN 10_1101-2021_01_07_425737 235 17 systems system NNS 10_1101-2021_01_07_425737 235 18 . . . 10_1101-2021_01_07_425737 236 1 Annu Annu NNP 10_1101-2021_01_07_425737 236 2 Rev Rev NNP 10_1101-2021_01_07_425737 236 3 Microbiol Microbiol NNP 10_1101-2021_01_07_425737 236 4 54 54 CD 10_1101-2021_01_07_425737 236 5 , , , 10_1101-2021_01_07_425737 236 6 735 735 CD 10_1101-2021_01_07_425737 236 7 - - HYPH 10_1101-2021_01_07_425737 236 8 774 774 CD 10_1101-2021_01_07_425737 236 9 ( ( -LRB- 10_1101-2021_01_07_425737 236 10 2000 2000 CD 10_1101-2021_01_07_425737 236 11 ) ) -RRB- 10_1101-2021_01_07_425737 236 12 . . . 10_1101-2021_01_07_425737 237 1 16 16 CD 10_1101-2021_01_07_425737 237 2 . . . 10_1101-2021_01_07_425737 238 1 Moya Moya NNP 10_1101-2021_01_07_425737 238 2 , , , 10_1101-2021_01_07_425737 238 3 A. A. NNP 10_1101-2021_01_07_425737 238 4 , , , 10_1101-2021_01_07_425737 238 5 Peretó Peretó NNP 10_1101-2021_01_07_425737 238 6 , , , 10_1101-2021_01_07_425737 238 7 J. J. NNP 10_1101-2021_01_07_425737 238 8 , , , 10_1101-2021_01_07_425737 238 9 Gil Gil NNP 10_1101-2021_01_07_425737 238 10 , , , 10_1101-2021_01_07_425737 238 11 R. R. NNP 10_1101-2021_01_07_425737 238 12 & & CC 10_1101-2021_01_07_425737 238 13 Latorre Latorre NNP 10_1101-2021_01_07_425737 238 14 , , , 10_1101-2021_01_07_425737 238 15 A. a. NN 10_1101-2021_01_07_425737 239 1 Learning learn VBG 10_1101-2021_01_07_425737 239 2 how how WRB 10_1101-2021_01_07_425737 239 3 to to TO 10_1101-2021_01_07_425737 239 4 live live VB 10_1101-2021_01_07_425737 239 5 together together RB 10_1101-2021_01_07_425737 239 6 : : : 10_1101-2021_01_07_425737 239 7 genomic genomic JJ 10_1101-2021_01_07_425737 239 8 insights insight NNS 10_1101-2021_01_07_425737 239 9 into into IN 10_1101-2021_01_07_425737 239 10 prokaryote prokaryote JJ 10_1101-2021_01_07_425737 239 11 - - HYPH 10_1101-2021_01_07_425737 239 12 animal animal NN 10_1101-2021_01_07_425737 239 13 symbioses symbiose NNS 10_1101-2021_01_07_425737 239 14 . . . 10_1101-2021_01_07_425737 240 1 Nat Nat NNP 10_1101-2021_01_07_425737 240 2 Rev Rev NNP 10_1101-2021_01_07_425737 240 3 Genet Genet NNP 10_1101-2021_01_07_425737 240 4 9 9 CD 10_1101-2021_01_07_425737 240 5 , , , 10_1101-2021_01_07_425737 240 6 218 218 CD 10_1101-2021_01_07_425737 240 7 - - HYPH 10_1101-2021_01_07_425737 240 8 229 229 CD 10_1101-2021_01_07_425737 240 9 ( ( -LRB- 10_1101-2021_01_07_425737 240 10 2008 2008 CD 10_1101-2021_01_07_425737 240 11 ) ) -RRB- 10_1101-2021_01_07_425737 240 12 . . . 10_1101-2021_01_07_425737 241 1 17 17 CD 10_1101-2021_01_07_425737 241 2 . . . 10_1101-2021_01_07_425737 242 1 Abby Abby NNP 10_1101-2021_01_07_425737 242 2 , , , 10_1101-2021_01_07_425737 242 3 S. S. NNP 10_1101-2021_01_07_425737 242 4 S. S. NNP 10_1101-2021_01_07_425737 242 5 & & CC 10_1101-2021_01_07_425737 242 6 Rocha Rocha NNP 10_1101-2021_01_07_425737 242 7 , , , 10_1101-2021_01_07_425737 242 8 E. E. NNP 10_1101-2021_01_07_425737 242 9 P. P. NNP 10_1101-2021_01_07_425737 242 10 The the DT 10_1101-2021_01_07_425737 242 11 non non JJ 10_1101-2021_01_07_425737 242 12 - - JJ 10_1101-2021_01_07_425737 242 13 flagellar flagellar JJ 10_1101-2021_01_07_425737 242 14 type type NN 10_1101-2021_01_07_425737 242 15 III iii CD 10_1101-2021_01_07_425737 242 16 secretion secretion NN 10_1101-2021_01_07_425737 242 17 system system NN 10_1101-2021_01_07_425737 242 18 evolved evolve VBD 10_1101-2021_01_07_425737 242 19 from from IN 10_1101-2021_01_07_425737 242 20 the the DT 10_1101-2021_01_07_425737 242 21 bacterial bacterial JJ 10_1101-2021_01_07_425737 242 22 flagellum flagellum NN 10_1101-2021_01_07_425737 242 23 and and CC 10_1101-2021_01_07_425737 242 24 diversified diversify VBN 10_1101-2021_01_07_425737 242 25 into into IN 10_1101-2021_01_07_425737 242 26 host host NN 10_1101-2021_01_07_425737 242 27 - - HYPH 10_1101-2021_01_07_425737 242 28 cell cell NN 10_1101-2021_01_07_425737 242 29 adapted adapt VBN 10_1101-2021_01_07_425737 242 30 systems system NNS 10_1101-2021_01_07_425737 242 31 . . . 10_1101-2021_01_07_425737 243 1 PLoS PLoS NNP 10_1101-2021_01_07_425737 243 2 Genet Genet NNP 10_1101-2021_01_07_425737 243 3 8 8 CD 10_1101-2021_01_07_425737 243 4 , , , 10_1101-2021_01_07_425737 243 5 e1002983 e1002983 NNP 10_1101-2021_01_07_425737 243 6 ( ( -LRB- 10_1101-2021_01_07_425737 243 7 2012 2012 CD 10_1101-2021_01_07_425737 243 8 ) ) -RRB- 10_1101-2021_01_07_425737 243 9 . . . 10_1101-2021_01_07_425737 244 1 18 18 CD 10_1101-2021_01_07_425737 244 2 . . . 10_1101-2021_01_07_425737 245 1 Marlovits Marlovits NNP 10_1101-2021_01_07_425737 245 2 , , , 10_1101-2021_01_07_425737 245 3 T. T. NNP 10_1101-2021_01_07_425737 245 4 C. C. NNP 10_1101-2021_01_07_425737 245 5 et et NNP 10_1101-2021_01_07_425737 245 6 al al NNP 10_1101-2021_01_07_425737 245 7 . . . 10_1101-2021_01_07_425737 246 1 Structural structural JJ 10_1101-2021_01_07_425737 246 2 insights insight NNS 10_1101-2021_01_07_425737 246 3 into into IN 10_1101-2021_01_07_425737 246 4 the the DT 10_1101-2021_01_07_425737 246 5 assembly assembly NN 10_1101-2021_01_07_425737 246 6 of of IN 10_1101-2021_01_07_425737 246 7 the the DT 10_1101-2021_01_07_425737 246 8 type type NN 10_1101-2021_01_07_425737 246 9 III III NNP 10_1101-2021_01_07_425737 246 10 secretion secretion NN 10_1101-2021_01_07_425737 246 11 needle needle NN 10_1101-2021_01_07_425737 246 12 complex complex NN 10_1101-2021_01_07_425737 246 13 . . . 10_1101-2021_01_07_425737 247 1 Science science NN 10_1101-2021_01_07_425737 247 2 306 306 CD 10_1101-2021_01_07_425737 247 3 , , , 10_1101-2021_01_07_425737 247 4 1040 1040 CD 10_1101-2021_01_07_425737 247 5 - - SYM 10_1101-2021_01_07_425737 247 6 1042 1042 CD 10_1101-2021_01_07_425737 247 7 ( ( -LRB- 10_1101-2021_01_07_425737 247 8 2004 2004 CD 10_1101-2021_01_07_425737 247 9 ) ) -RRB- 10_1101-2021_01_07_425737 247 10 . . . 10_1101-2021_01_07_425737 248 1 19 19 CD 10_1101-2021_01_07_425737 248 2 . . . 10_1101-2021_01_07_425737 249 1 Liu Liu NNP 10_1101-2021_01_07_425737 249 2 , , , 10_1101-2021_01_07_425737 249 3 R. R. NNP 10_1101-2021_01_07_425737 249 4 & & CC 10_1101-2021_01_07_425737 249 5 Ochman Ochman NNP 10_1101-2021_01_07_425737 249 6 , , , 10_1101-2021_01_07_425737 249 7 H. H. NNP 10_1101-2021_01_07_425737 249 8 Stepwise Stepwise NNP 10_1101-2021_01_07_425737 249 9 formation formation NN 10_1101-2021_01_07_425737 249 10 of of IN 10_1101-2021_01_07_425737 249 11 the the DT 10_1101-2021_01_07_425737 249 12 bacterial bacterial JJ 10_1101-2021_01_07_425737 249 13 flagellar flagellar JJ 10_1101-2021_01_07_425737 249 14 system system NN 10_1101-2021_01_07_425737 249 15 . . . 10_1101-2021_01_07_425737 250 1 Proc Proc NNP 10_1101-2021_01_07_425737 250 2 Natl Natl NNP 10_1101-2021_01_07_425737 250 3 Acad Acad NNP 10_1101-2021_01_07_425737 250 4 Sci Sci NNP 10_1101-2021_01_07_425737 250 5 U U NNP 10_1101-2021_01_07_425737 250 6 S S NNP 10_1101-2021_01_07_425737 250 7 A A NNP 10_1101-2021_01_07_425737 250 8 104 104 CD 10_1101-2021_01_07_425737 250 9 , , , 10_1101-2021_01_07_425737 250 10 7116 7116 CD 10_1101-2021_01_07_425737 250 11 - - SYM 10_1101-2021_01_07_425737 250 12 7121 7121 CD 10_1101-2021_01_07_425737 250 13 ( ( -LRB- 10_1101-2021_01_07_425737 250 14 2007 2007 CD 10_1101-2021_01_07_425737 250 15 ) ) -RRB- 10_1101-2021_01_07_425737 250 16 . . . 10_1101-2021_01_07_425737 251 1 20 20 CD 10_1101-2021_01_07_425737 251 2 . . . 10_1101-2021_01_07_425737 252 1 Ince Ince NNP 10_1101-2021_01_07_425737 252 2 , , , 10_1101-2021_01_07_425737 252 3 D. D. NNP 10_1101-2021_01_07_425737 252 4 , , , 10_1101-2021_01_07_425737 252 5 Sutterwala Sutterwala NNP 10_1101-2021_01_07_425737 252 6 , , , 10_1101-2021_01_07_425737 252 7 F. F. NNP 10_1101-2021_01_07_425737 252 8 S. S. NNP 10_1101-2021_01_07_425737 252 9 & & CC 10_1101-2021_01_07_425737 252 10 Yahr Yahr NNP 10_1101-2021_01_07_425737 252 11 , , , 10_1101-2021_01_07_425737 252 12 T. T. NNP 10_1101-2021_01_07_425737 252 13 L. L. NNP 10_1101-2021_01_07_425737 252 14 Secretion Secretion NNP 10_1101-2021_01_07_425737 252 15 of of IN 10_1101-2021_01_07_425737 252 16 Flagellar Flagellar NNP 10_1101-2021_01_07_425737 252 17 Proteins Proteins NNPS 10_1101-2021_01_07_425737 252 18 by by IN 10_1101-2021_01_07_425737 252 19 the the DT 10_1101-2021_01_07_425737 252 20 Pseudomonas Pseudomonas NNP 10_1101-2021_01_07_425737 252 21 aeruginosa aeruginosa NNP 10_1101-2021_01_07_425737 252 22 Type Type NNP 10_1101-2021_01_07_425737 252 23 III III NNP 10_1101-2021_01_07_425737 252 24 Secretion Secretion NNP 10_1101-2021_01_07_425737 252 25 - - HYPH 10_1101-2021_01_07_425737 252 26 Injectisome Injectisome NNP 10_1101-2021_01_07_425737 252 27 System System NNP 10_1101-2021_01_07_425737 252 28 . . . 10_1101-2021_01_07_425737 253 1 Journal Journal NNP 10_1101-2021_01_07_425737 253 2 of of IN 10_1101-2021_01_07_425737 253 3 Bacteriology Bacteriology NNP 10_1101-2021_01_07_425737 253 4 197 197 CD 10_1101-2021_01_07_425737 253 5 , , , 10_1101-2021_01_07_425737 253 6 2003 2003 CD 10_1101-2021_01_07_425737 253 7 - - SYM 10_1101-2021_01_07_425737 253 8 2011 2011 CD 10_1101-2021_01_07_425737 253 9 ( ( -LRB- 10_1101-2021_01_07_425737 253 10 2015 2015 CD 10_1101-2021_01_07_425737 253 11 ) ) -RRB- 10_1101-2021_01_07_425737 253 12 . . . 10_1101-2021_01_07_425737 254 1 ( ( -LRB- 10_1101-2021_01_07_425737 254 2 which which WDT 10_1101-2021_01_07_425737 254 3 was be VBD 10_1101-2021_01_07_425737 254 4 not not RB 10_1101-2021_01_07_425737 254 5 certified certify VBN 10_1101-2021_01_07_425737 254 6 by by IN 10_1101-2021_01_07_425737 254 7 peer peer NN 10_1101-2021_01_07_425737 254 8 review review NN 10_1101-2021_01_07_425737 254 9 ) ) -RRB- 10_1101-2021_01_07_425737 254 10 is be VBZ 10_1101-2021_01_07_425737 254 11 the the DT 10_1101-2021_01_07_425737 254 12 author author NN 10_1101-2021_01_07_425737 254 13 / / SYM 10_1101-2021_01_07_425737 254 14 funder funder NN 10_1101-2021_01_07_425737 254 15 . . . 10_1101-2021_01_07_425737 255 1 All all DT 10_1101-2021_01_07_425737 255 2 rights right NNS 10_1101-2021_01_07_425737 255 3 reserved reserve VBD 10_1101-2021_01_07_425737 255 4 . . . 10_1101-2021_01_07_425737 256 1 No no DT 10_1101-2021_01_07_425737 256 2 reuse reuse NN 10_1101-2021_01_07_425737 256 3 allowed allow VBN 10_1101-2021_01_07_425737 256 4 without without IN 10_1101-2021_01_07_425737 256 5 permission permission NN 10_1101-2021_01_07_425737 256 6 . . . 10_1101-2021_01_07_425737 257 1 The the DT 10_1101-2021_01_07_425737 257 2 copyright copyright NN 10_1101-2021_01_07_425737 257 3 holder holder NN 10_1101-2021_01_07_425737 257 4 for for IN 10_1101-2021_01_07_425737 257 5 this this DT 10_1101-2021_01_07_425737 257 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 257 7 version version NN 10_1101-2021_01_07_425737 257 8 posted post VBD 10_1101-2021_01_07_425737 257 9 January January NNP 10_1101-2021_01_07_425737 257 10 8 8 CD 10_1101-2021_01_07_425737 257 11 , , , 10_1101-2021_01_07_425737 257 12 2021 2021 CD 10_1101-2021_01_07_425737 257 13 . . . 10_1101-2021_01_07_425737 257 14 ; ; : 10_1101-2021_01_07_425737 257 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 257 16 : : : 10_1101-2021_01_07_425737 257 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 257 18 preprint preprint NN 10_1101-2021_01_07_425737 257 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 NNP 10_1101-2021_01_07_425737 257 20 21 21 CD 10_1101-2021_01_07_425737 257 21 . . . 10_1101-2021_01_07_425737 258 1 Young Young NNP 10_1101-2021_01_07_425737 258 2 , , , 10_1101-2021_01_07_425737 258 3 G. G. NNP 10_1101-2021_01_07_425737 258 4 M. M. NNP 10_1101-2021_01_07_425737 258 5 , , , 10_1101-2021_01_07_425737 258 6 Schmiel Schmiel NNP 10_1101-2021_01_07_425737 258 7 , , , 10_1101-2021_01_07_425737 258 8 D. D. NNP 10_1101-2021_01_07_425737 258 9 H. H. NNP 10_1101-2021_01_07_425737 258 10 & & CC 10_1101-2021_01_07_425737 258 11 Miller Miller NNP 10_1101-2021_01_07_425737 258 12 , , , 10_1101-2021_01_07_425737 258 13 V. V. NNP 10_1101-2021_01_07_425737 258 14 L. L. NNP 10_1101-2021_01_07_425737 258 15 A a DT 10_1101-2021_01_07_425737 258 16 new new JJ 10_1101-2021_01_07_425737 258 17 pathway pathway NN 10_1101-2021_01_07_425737 258 18 for for IN 10_1101-2021_01_07_425737 258 19 the the DT 10_1101-2021_01_07_425737 258 20 secretion secretion NN 10_1101-2021_01_07_425737 258 21 of of IN 10_1101-2021_01_07_425737 258 22 virulence virulence NN 10_1101-2021_01_07_425737 258 23 factors factor NNS 10_1101-2021_01_07_425737 258 24 by by IN 10_1101-2021_01_07_425737 258 25 bacteria bacteria NNS 10_1101-2021_01_07_425737 258 26 : : : 10_1101-2021_01_07_425737 258 27 the the DT 10_1101-2021_01_07_425737 258 28 flagellar flagellar NNP 10_1101-2021_01_07_425737 258 29 export export NN 10_1101-2021_01_07_425737 258 30 apparatus apparatus NN 10_1101-2021_01_07_425737 258 31 functions function NNS 10_1101-2021_01_07_425737 258 32 as as IN 10_1101-2021_01_07_425737 258 33 a a DT 10_1101-2021_01_07_425737 258 34 protein protein NN 10_1101-2021_01_07_425737 258 35 - - HYPH 10_1101-2021_01_07_425737 258 36 secretion secretion NN 10_1101-2021_01_07_425737 258 37 system system NN 10_1101-2021_01_07_425737 258 38 . . . 10_1101-2021_01_07_425737 259 1 Proc Proc NNP 10_1101-2021_01_07_425737 259 2 Natl Natl NNP 10_1101-2021_01_07_425737 259 3 Acad Acad NNP 10_1101-2021_01_07_425737 259 4 Sci Sci NNP 10_1101-2021_01_07_425737 259 5 U U NNP 10_1101-2021_01_07_425737 259 6 S S NNP 10_1101-2021_01_07_425737 259 7 A A NNP 10_1101-2021_01_07_425737 259 8 96 96 CD 10_1101-2021_01_07_425737 259 9 , , , 10_1101-2021_01_07_425737 259 10 6456 6456 CD 10_1101-2021_01_07_425737 259 11 - - SYM 10_1101-2021_01_07_425737 259 12 6461 6461 CD 10_1101-2021_01_07_425737 259 13 ( ( -LRB- 10_1101-2021_01_07_425737 259 14 1999 1999 CD 10_1101-2021_01_07_425737 259 15 ) ) -RRB- 10_1101-2021_01_07_425737 259 16 . . . 10_1101-2021_01_07_425737 260 1 22 22 CD 10_1101-2021_01_07_425737 260 2 . . . 10_1101-2021_01_07_425737 261 1 Irikura Irikura NNP 10_1101-2021_01_07_425737 261 2 , , , 10_1101-2021_01_07_425737 261 3 V. V. NNP 10_1101-2021_01_07_425737 261 4 M. M. NNP 10_1101-2021_01_07_425737 261 5 , , , 10_1101-2021_01_07_425737 261 6 Kihara Kihara NNP 10_1101-2021_01_07_425737 261 7 , , , 10_1101-2021_01_07_425737 261 8 M. M. NNP 10_1101-2021_01_07_425737 261 9 , , , 10_1101-2021_01_07_425737 261 10 Yamaguchi Yamaguchi NNP 10_1101-2021_01_07_425737 261 11 , , , 10_1101-2021_01_07_425737 261 12 S. S. NNP 10_1101-2021_01_07_425737 261 13 , , , 10_1101-2021_01_07_425737 261 14 Sockett Sockett NNP 10_1101-2021_01_07_425737 261 15 , , , 10_1101-2021_01_07_425737 261 16 H. H. NNP 10_1101-2021_01_07_425737 261 17 & & CC 10_1101-2021_01_07_425737 261 18 Macnab Macnab NNP 10_1101-2021_01_07_425737 261 19 , , , 10_1101-2021_01_07_425737 261 20 R. R. NNP 10_1101-2021_01_07_425737 261 21 M. M. NNP 10_1101-2021_01_07_425737 261 22 Salmonella Salmonella NNP 10_1101-2021_01_07_425737 261 23 typhimurium typhimurium NN 10_1101-2021_01_07_425737 261 24 fliG fliG NNS 10_1101-2021_01_07_425737 261 25 and and CC 10_1101-2021_01_07_425737 261 26 fliN flin JJ 10_1101-2021_01_07_425737 261 27 mutations mutation NNS 10_1101-2021_01_07_425737 261 28 causing cause VBG 10_1101-2021_01_07_425737 261 29 defects defect NNS 10_1101-2021_01_07_425737 261 30 in in IN 10_1101-2021_01_07_425737 261 31 assembly assembly NN 10_1101-2021_01_07_425737 261 32 , , , 10_1101-2021_01_07_425737 261 33 rotation rotation NN 10_1101-2021_01_07_425737 261 34 , , , 10_1101-2021_01_07_425737 261 35 and and CC 10_1101-2021_01_07_425737 261 36 switching switching NN 10_1101-2021_01_07_425737 261 37 of of IN 10_1101-2021_01_07_425737 261 38 the the DT 10_1101-2021_01_07_425737 261 39 flagellar flagellar JJ 10_1101-2021_01_07_425737 261 40 motor motor NN 10_1101-2021_01_07_425737 261 41 . . . 10_1101-2021_01_07_425737 262 1 J J NNP 10_1101-2021_01_07_425737 262 2 Bacteriol Bacteriol NNP 10_1101-2021_01_07_425737 262 3 175 175 CD 10_1101-2021_01_07_425737 262 4 , , , 10_1101-2021_01_07_425737 262 5 802 802 CD 10_1101-2021_01_07_425737 262 6 - - HYPH 10_1101-2021_01_07_425737 262 7 810 810 CD 10_1101-2021_01_07_425737 262 8 ( ( -LRB- 10_1101-2021_01_07_425737 262 9 1993 1993 CD 10_1101-2021_01_07_425737 262 10 ) ) -RRB- 10_1101-2021_01_07_425737 262 11 . . . 10_1101-2021_01_07_425737 263 1 23 23 CD 10_1101-2021_01_07_425737 263 2 . . . 10_1101-2021_01_07_425737 264 1 Minamino Minamino NNP 10_1101-2021_01_07_425737 264 2 , , , 10_1101-2021_01_07_425737 264 3 T. T. NNP 10_1101-2021_01_07_425737 264 4 & & CC 10_1101-2021_01_07_425737 264 5 Macnab Macnab NNP 10_1101-2021_01_07_425737 264 6 , , , 10_1101-2021_01_07_425737 264 7 R. R. NNP 10_1101-2021_01_07_425737 264 8 M. M. NNP 10_1101-2021_01_07_425737 264 9 Components Components NNP 10_1101-2021_01_07_425737 264 10 of of IN 10_1101-2021_01_07_425737 264 11 the the DT 10_1101-2021_01_07_425737 264 12 Salmonella Salmonella NNP 10_1101-2021_01_07_425737 264 13 flagellar flagellar JJ 10_1101-2021_01_07_425737 264 14 export export NN 10_1101-2021_01_07_425737 264 15 apparatus apparatus NN 10_1101-2021_01_07_425737 264 16 and and CC 10_1101-2021_01_07_425737 264 17 classification classification NN 10_1101-2021_01_07_425737 264 18 of of IN 10_1101-2021_01_07_425737 264 19 export export NN 10_1101-2021_01_07_425737 264 20 substrates substrate NNS 10_1101-2021_01_07_425737 264 21 . . . 10_1101-2021_01_07_425737 265 1 J J NNP 10_1101-2021_01_07_425737 265 2 Bacteriol Bacteriol NNP 10_1101-2021_01_07_425737 265 3 181 181 CD 10_1101-2021_01_07_425737 265 4 , , , 10_1101-2021_01_07_425737 265 5 1388 1388 CD 10_1101-2021_01_07_425737 265 6 - - SYM 10_1101-2021_01_07_425737 265 7 1394 1394 CD 10_1101-2021_01_07_425737 265 8 ( ( -LRB- 10_1101-2021_01_07_425737 265 9 1999 1999 CD 10_1101-2021_01_07_425737 265 10 ) ) -RRB- 10_1101-2021_01_07_425737 265 11 . . . 10_1101-2021_01_07_425737 266 1 24 24 CD 10_1101-2021_01_07_425737 266 2 . . . 10_1101-2021_01_07_425737 267 1 Konkel Konkel NNP 10_1101-2021_01_07_425737 267 2 , , , 10_1101-2021_01_07_425737 267 3 M. M. NNP 10_1101-2021_01_07_425737 267 4 E. E. NNP 10_1101-2021_01_07_425737 267 5 et et NNP 10_1101-2021_01_07_425737 267 6 al al NNP 10_1101-2021_01_07_425737 267 7 . . . 10_1101-2021_01_07_425737 268 1 Secretion secretion NN 10_1101-2021_01_07_425737 268 2 of of IN 10_1101-2021_01_07_425737 268 3 virulence virulence NN 10_1101-2021_01_07_425737 268 4 proteins protein NNS 10_1101-2021_01_07_425737 268 5 from from IN 10_1101-2021_01_07_425737 268 6 Campylobacter Campylobacter NNP 10_1101-2021_01_07_425737 268 7 jejuni jejuni NN 10_1101-2021_01_07_425737 268 8 is be VBZ 10_1101-2021_01_07_425737 268 9 dependent dependent JJ 10_1101-2021_01_07_425737 268 10 on on IN 10_1101-2021_01_07_425737 268 11 a a DT 10_1101-2021_01_07_425737 268 12 functional functional JJ 10_1101-2021_01_07_425737 268 13 flagellar flagellar JJ 10_1101-2021_01_07_425737 268 14 export export NN 10_1101-2021_01_07_425737 268 15 apparatus apparatus NN 10_1101-2021_01_07_425737 268 16 . . . 10_1101-2021_01_07_425737 269 1 J J NNP 10_1101-2021_01_07_425737 269 2 Bacteriol Bacteriol NNP 10_1101-2021_01_07_425737 269 3 186 186 CD 10_1101-2021_01_07_425737 269 4 , , , 10_1101-2021_01_07_425737 269 5 3296 3296 CD 10_1101-2021_01_07_425737 269 6 - - SYM 10_1101-2021_01_07_425737 269 7 3303 3303 CD 10_1101-2021_01_07_425737 269 8 ( ( -LRB- 10_1101-2021_01_07_425737 269 9 2004 2004 CD 10_1101-2021_01_07_425737 269 10 ) ) -RRB- 10_1101-2021_01_07_425737 269 11 . . . 10_1101-2021_01_07_425737 270 1 25 25 CD 10_1101-2021_01_07_425737 270 2 . . . 10_1101-2021_01_07_425737 271 1 Scanlan Scanlan NNP 10_1101-2021_01_07_425737 271 2 , , , 10_1101-2021_01_07_425737 271 3 E. E. NNP 10_1101-2021_01_07_425737 271 4 , , , 10_1101-2021_01_07_425737 271 5 Yu Yu NNP 10_1101-2021_01_07_425737 271 6 , , , 10_1101-2021_01_07_425737 271 7 L. L. NNP 10_1101-2021_01_07_425737 271 8 , , , 10_1101-2021_01_07_425737 271 9 Maskell Maskell NNP 10_1101-2021_01_07_425737 271 10 , , , 10_1101-2021_01_07_425737 271 11 D. D. NNP 10_1101-2021_01_07_425737 271 12 , , , 10_1101-2021_01_07_425737 271 13 Choudhary Choudhary NNP 10_1101-2021_01_07_425737 271 14 , , , 10_1101-2021_01_07_425737 271 15 J. J. NNP 10_1101-2021_01_07_425737 272 1 & & CC 10_1101-2021_01_07_425737 272 2 Grant Grant NNP 10_1101-2021_01_07_425737 272 3 , , , 10_1101-2021_01_07_425737 272 4 A. a. NN 10_1101-2021_01_07_425737 273 1 A a DT 10_1101-2021_01_07_425737 273 2 quantitative quantitative JJ 10_1101-2021_01_07_425737 273 3 proteomic proteomic JJ 10_1101-2021_01_07_425737 273 4 screen screen NN 10_1101-2021_01_07_425737 273 5 of of IN 10_1101-2021_01_07_425737 273 6 the the DT 10_1101-2021_01_07_425737 273 7 Campylobacter Campylobacter NNP 10_1101-2021_01_07_425737 273 8 jejuni jejuni NNP 10_1101-2021_01_07_425737 273 9 flagellar flagellar NN 10_1101-2021_01_07_425737 273 10 - - HYPH 10_1101-2021_01_07_425737 273 11 dependent dependent JJ 10_1101-2021_01_07_425737 273 12 secretome secretome NN 10_1101-2021_01_07_425737 273 13 . . . 10_1101-2021_01_07_425737 274 1 J J NNP 10_1101-2021_01_07_425737 274 2 Proteomics Proteomics NNP 10_1101-2021_01_07_425737 274 3 152 152 CD 10_1101-2021_01_07_425737 274 4 , , , 10_1101-2021_01_07_425737 274 5 181 181 CD 10_1101-2021_01_07_425737 274 6 - - SYM 10_1101-2021_01_07_425737 274 7 187 187 CD 10_1101-2021_01_07_425737 274 8 ( ( -LRB- 10_1101-2021_01_07_425737 274 9 2017 2017 CD 10_1101-2021_01_07_425737 274 10 ) ) -RRB- 10_1101-2021_01_07_425737 274 11 . . . 10_1101-2021_01_07_425737 275 1 26 26 CD 10_1101-2021_01_07_425737 275 2 . . . 10_1101-2021_01_07_425737 276 1 López López NNP 10_1101-2021_01_07_425737 276 2 - - HYPH 10_1101-2021_01_07_425737 276 3 Sánchez Sánchez NNP 10_1101-2021_01_07_425737 276 4 , , , 10_1101-2021_01_07_425737 276 5 M. M. NNP 10_1101-2021_01_07_425737 276 6 J. J. NNP 10_1101-2021_01_07_425737 276 7 et et NNP 10_1101-2021_01_07_425737 276 8 al al NNP 10_1101-2021_01_07_425737 276 9 . . . 10_1101-2021_01_07_425737 277 1 Evolutionary evolutionary JJ 10_1101-2021_01_07_425737 277 2 convergence convergence NN 10_1101-2021_01_07_425737 277 3 and and CC 10_1101-2021_01_07_425737 277 4 nitrogen nitrogen NN 10_1101-2021_01_07_425737 277 5 metabolism metabolism NN 10_1101-2021_01_07_425737 277 6 in in IN 10_1101-2021_01_07_425737 277 7 Blattabacterium Blattabacterium NNP 10_1101-2021_01_07_425737 277 8 strain strain NN 10_1101-2021_01_07_425737 277 9 Bge Bge NNP 10_1101-2021_01_07_425737 277 10 , , , 10_1101-2021_01_07_425737 277 11 primary primary JJ 10_1101-2021_01_07_425737 277 12 endosymbiont endosymbiont NN 10_1101-2021_01_07_425737 277 13 of of IN 10_1101-2021_01_07_425737 277 14 the the DT 10_1101-2021_01_07_425737 277 15 cockroach cockroach NN 10_1101-2021_01_07_425737 277 16 Blattella Blattella NNP 10_1101-2021_01_07_425737 277 17 germanica germanica NNP 10_1101-2021_01_07_425737 277 18 . . . 10_1101-2021_01_07_425737 278 1 PLoS PLoS : 10_1101-2021_01_07_425737 278 2 Genet Genet NNP 10_1101-2021_01_07_425737 278 3 5 5 CD 10_1101-2021_01_07_425737 278 4 , , , 10_1101-2021_01_07_425737 278 5 e1000721 e1000721 NNP 10_1101-2021_01_07_425737 278 6 ( ( -LRB- 10_1101-2021_01_07_425737 278 7 2009 2009 CD 10_1101-2021_01_07_425737 278 8 ) ) -RRB- 10_1101-2021_01_07_425737 278 9 . . . 10_1101-2021_01_07_425737 279 1 27 27 CD 10_1101-2021_01_07_425737 279 2 . . . 10_1101-2021_01_07_425737 280 1 Tegunov Tegunov NNP 10_1101-2021_01_07_425737 280 2 , , , 10_1101-2021_01_07_425737 280 3 D. D. NNP 10_1101-2021_01_07_425737 280 4 & & CC 10_1101-2021_01_07_425737 280 5 Cramer Cramer NNP 10_1101-2021_01_07_425737 280 6 , , , 10_1101-2021_01_07_425737 280 7 P. P. NNP 10_1101-2021_01_07_425737 280 8 Real real JJ 10_1101-2021_01_07_425737 280 9 - - HYPH 10_1101-2021_01_07_425737 280 10 time time NN 10_1101-2021_01_07_425737 280 11 cryo cryo NN 10_1101-2021_01_07_425737 280 12 - - HYPH 10_1101-2021_01_07_425737 280 13 electron electron NN 10_1101-2021_01_07_425737 280 14 microscopy microscopy JJ 10_1101-2021_01_07_425737 280 15 data datum NNS 10_1101-2021_01_07_425737 280 16 preprocessing preprocesse VBG 10_1101-2021_01_07_425737 280 17 with with IN 10_1101-2021_01_07_425737 280 18 Warp Warp NNP 10_1101-2021_01_07_425737 280 19 . . . 10_1101-2021_01_07_425737 281 1 Nat Nat NNP 10_1101-2021_01_07_425737 281 2 Methods Methods NNP 10_1101-2021_01_07_425737 281 3 16 16 CD 10_1101-2021_01_07_425737 281 4 , , , 10_1101-2021_01_07_425737 281 5 1146 1146 CD 10_1101-2021_01_07_425737 281 6 - - SYM 10_1101-2021_01_07_425737 281 7 1152 1152 CD 10_1101-2021_01_07_425737 281 8 ( ( -LRB- 10_1101-2021_01_07_425737 281 9 2019 2019 CD 10_1101-2021_01_07_425737 281 10 ) ) -RRB- 10_1101-2021_01_07_425737 281 11 . . . 10_1101-2021_01_07_425737 282 1 28 28 CD 10_1101-2021_01_07_425737 282 2 . . . 10_1101-2021_01_07_425737 283 1 Braendle Braendle NNP 10_1101-2021_01_07_425737 283 2 , , , 10_1101-2021_01_07_425737 283 3 C. C. NNP 10_1101-2021_01_07_425737 283 4 et et NNP 10_1101-2021_01_07_425737 283 5 al al NNP 10_1101-2021_01_07_425737 283 6 . . . 10_1101-2021_01_07_425737 284 1 Developmental developmental JJ 10_1101-2021_01_07_425737 284 2 origin origin NN 10_1101-2021_01_07_425737 284 3 and and CC 10_1101-2021_01_07_425737 284 4 evolution evolution NN 10_1101-2021_01_07_425737 284 5 of of IN 10_1101-2021_01_07_425737 284 6 bacteriocytes bacteriocyte NNS 10_1101-2021_01_07_425737 284 7 in in IN 10_1101-2021_01_07_425737 284 8 the the DT 10_1101-2021_01_07_425737 284 9 aphid- aphid- NN 10_1101-2021_01_07_425737 284 10 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 284 11 symbiosis symbiosis NN 10_1101-2021_01_07_425737 284 12 . . . 10_1101-2021_01_07_425737 285 1 PLoS PLoS NNP 10_1101-2021_01_07_425737 285 2 Biol Biol NNP 10_1101-2021_01_07_425737 285 3 1 1 CD 10_1101-2021_01_07_425737 285 4 , , , 10_1101-2021_01_07_425737 285 5 E21 E21 NNP 10_1101-2021_01_07_425737 285 6 ( ( -LRB- 10_1101-2021_01_07_425737 285 7 2003 2003 CD 10_1101-2021_01_07_425737 285 8 ) ) -RRB- 10_1101-2021_01_07_425737 285 9 . . . 10_1101-2021_01_07_425737 286 1 29 29 CD 10_1101-2021_01_07_425737 286 2 . . . 10_1101-2021_01_07_425737 287 1 Miura Miura NNP 10_1101-2021_01_07_425737 287 2 , , , 10_1101-2021_01_07_425737 287 3 T. T. NNP 10_1101-2021_01_07_425737 287 4 et et NNP 10_1101-2021_01_07_425737 287 5 al al NNP 10_1101-2021_01_07_425737 287 6 . . . 10_1101-2021_01_07_425737 288 1 A a DT 10_1101-2021_01_07_425737 288 2 comparison comparison NN 10_1101-2021_01_07_425737 288 3 of of IN 10_1101-2021_01_07_425737 288 4 parthenogenetic parthenogenetic JJ 10_1101-2021_01_07_425737 288 5 and and CC 10_1101-2021_01_07_425737 288 6 sexual sexual JJ 10_1101-2021_01_07_425737 288 7 embryogenesis embryogenesis NN 10_1101-2021_01_07_425737 288 8 of of IN 10_1101-2021_01_07_425737 288 9 the the DT 10_1101-2021_01_07_425737 288 10 pea pea NN 10_1101-2021_01_07_425737 288 11 aphid aphid NNP 10_1101-2021_01_07_425737 288 12 Acyrthosiphon Acyrthosiphon NNP 10_1101-2021_01_07_425737 288 13 pisum pisum NN 10_1101-2021_01_07_425737 288 14 ( ( -LRB- 10_1101-2021_01_07_425737 288 15 Hemiptera Hemiptera NNP 10_1101-2021_01_07_425737 288 16 : : : 10_1101-2021_01_07_425737 288 17 Aphidoidea Aphidoidea NNP 10_1101-2021_01_07_425737 288 18 ) ) -RRB- 10_1101-2021_01_07_425737 288 19 . . . 10_1101-2021_01_07_425737 289 1 J J NNP 10_1101-2021_01_07_425737 289 2 Exp Exp NNP 10_1101-2021_01_07_425737 289 3 Zool Zool NNP 10_1101-2021_01_07_425737 289 4 B B NNP 10_1101-2021_01_07_425737 289 5 Mol Mol NNP 10_1101-2021_01_07_425737 289 6 Dev Dev NNP 10_1101-2021_01_07_425737 289 7 Evol Evol NNP 10_1101-2021_01_07_425737 289 8 295 295 CD 10_1101-2021_01_07_425737 289 9 , , , 10_1101-2021_01_07_425737 289 10 59- 59- NNP 10_1101-2021_01_07_425737 289 11 81 81 CD 10_1101-2021_01_07_425737 289 12 ( ( -LRB- 10_1101-2021_01_07_425737 289 13 2003 2003 CD 10_1101-2021_01_07_425737 289 14 ) ) -RRB- 10_1101-2021_01_07_425737 289 15 . . . 10_1101-2021_01_07_425737 290 1 30 30 CD 10_1101-2021_01_07_425737 290 2 . . . 10_1101-2021_01_07_425737 291 1 Smith Smith NNP 10_1101-2021_01_07_425737 291 2 , , , 10_1101-2021_01_07_425737 291 3 T. T. NNP 10_1101-2021_01_07_425737 291 4 E. E. NNP 10_1101-2021_01_07_425737 291 5 & & CC 10_1101-2021_01_07_425737 291 6 Moran Moran NNP 10_1101-2021_01_07_425737 291 7 , , , 10_1101-2021_01_07_425737 291 8 N. N. NNP 10_1101-2021_01_07_425737 291 9 A. a. NN 10_1101-2021_01_07_425737 292 1 Coordination coordination NN 10_1101-2021_01_07_425737 292 2 of of IN 10_1101-2021_01_07_425737 292 3 host host NN 10_1101-2021_01_07_425737 292 4 and and CC 10_1101-2021_01_07_425737 292 5 symbiont symbiont JJ 10_1101-2021_01_07_425737 292 6 gene gene NN 10_1101-2021_01_07_425737 292 7 expression expression NN 10_1101-2021_01_07_425737 292 8 reveals reveal VBZ 10_1101-2021_01_07_425737 292 9 a a DT 10_1101-2021_01_07_425737 292 10 metabolic metabolic JJ 10_1101-2021_01_07_425737 292 11 tug tug NN 10_1101-2021_01_07_425737 292 12 - - HYPH 10_1101-2021_01_07_425737 292 13 of of IN 10_1101-2021_01_07_425737 292 14 - - HYPH 10_1101-2021_01_07_425737 292 15 war war NN 10_1101-2021_01_07_425737 292 16 between between IN 10_1101-2021_01_07_425737 292 17 aphids aphids NNP 10_1101-2021_01_07_425737 292 18 and and CC 10_1101-2021_01_07_425737 292 19 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 292 20 . . . 10_1101-2021_01_07_425737 293 1 Proc Proc NNP 10_1101-2021_01_07_425737 293 2 Natl Natl NNP 10_1101-2021_01_07_425737 293 3 Acad Acad NNP 10_1101-2021_01_07_425737 293 4 Sci Sci NNP 10_1101-2021_01_07_425737 293 5 U U NNP 10_1101-2021_01_07_425737 293 6 S S NNP 10_1101-2021_01_07_425737 293 7 A A NNP 10_1101-2021_01_07_425737 293 8 117 117 CD 10_1101-2021_01_07_425737 293 9 , , , 10_1101-2021_01_07_425737 293 10 2113- 2113- CD 10_1101-2021_01_07_425737 293 11 2121 2121 CD 10_1101-2021_01_07_425737 293 12 ( ( -LRB- 10_1101-2021_01_07_425737 293 13 2020 2020 CD 10_1101-2021_01_07_425737 293 14 ) ) -RRB- 10_1101-2021_01_07_425737 293 15 . . . 10_1101-2021_01_07_425737 294 1 31 31 CD 10_1101-2021_01_07_425737 294 2 . . . 10_1101-2021_01_07_425737 295 1 Shimomura Shimomura NNP 10_1101-2021_01_07_425737 295 2 , , , 10_1101-2021_01_07_425737 295 3 S. S. NNP 10_1101-2021_01_07_425737 295 4 , , , 10_1101-2021_01_07_425737 295 5 Shigenobu Shigenobu NNP 10_1101-2021_01_07_425737 295 6 , , , 10_1101-2021_01_07_425737 295 7 S. S. NNP 10_1101-2021_01_07_425737 295 8 , , , 10_1101-2021_01_07_425737 295 9 Morioka Morioka NNP 10_1101-2021_01_07_425737 295 10 , , , 10_1101-2021_01_07_425737 295 11 M. M. NNP 10_1101-2021_01_07_425737 295 12 & & CC 10_1101-2021_01_07_425737 295 13 Ishikawa Ishikawa NNP 10_1101-2021_01_07_425737 295 14 , , , 10_1101-2021_01_07_425737 295 15 H. H. NNP 10_1101-2021_01_07_425737 295 16 An an DT 10_1101-2021_01_07_425737 295 17 experimental experimental JJ 10_1101-2021_01_07_425737 295 18 validation validation NN 10_1101-2021_01_07_425737 295 19 of of IN 10_1101-2021_01_07_425737 295 20 orphan orphan NN 10_1101-2021_01_07_425737 295 21 genes gene NNS 10_1101-2021_01_07_425737 295 22 of of IN 10_1101-2021_01_07_425737 295 23 Buchnera Buchnera NNP 10_1101-2021_01_07_425737 295 24 , , , 10_1101-2021_01_07_425737 295 25 a a DT 10_1101-2021_01_07_425737 295 26 symbiont symbiont NN 10_1101-2021_01_07_425737 295 27 of of IN 10_1101-2021_01_07_425737 295 28 aphids aphid NNS 10_1101-2021_01_07_425737 295 29 . . . 10_1101-2021_01_07_425737 296 1 Biochem Biochem NNP 10_1101-2021_01_07_425737 296 2 Biophys Biophys NNP 10_1101-2021_01_07_425737 296 3 Res Res NNP 10_1101-2021_01_07_425737 296 4 Commun Commun NNP 10_1101-2021_01_07_425737 296 5 292 292 CD 10_1101-2021_01_07_425737 296 6 , , , 10_1101-2021_01_07_425737 296 7 263 263 CD 10_1101-2021_01_07_425737 296 8 - - SYM 10_1101-2021_01_07_425737 296 9 267 267 CD 10_1101-2021_01_07_425737 296 10 ( ( -LRB- 10_1101-2021_01_07_425737 296 11 2002 2002 CD 10_1101-2021_01_07_425737 296 12 ) ) -RRB- 10_1101-2021_01_07_425737 296 13 . . . 10_1101-2021_01_07_425737 297 1 32 32 CD 10_1101-2021_01_07_425737 297 2 . . . 10_1101-2021_01_07_425737 298 1 Koga Koga NNP 10_1101-2021_01_07_425737 298 2 , , , 10_1101-2021_01_07_425737 298 3 R. R. NNP 10_1101-2021_01_07_425737 298 4 , , , 10_1101-2021_01_07_425737 298 5 Meng Meng NNP 10_1101-2021_01_07_425737 298 6 , , , 10_1101-2021_01_07_425737 298 7 X. X. NNP 10_1101-2021_01_07_425737 298 8 Y. Y. NNP 10_1101-2021_01_07_425737 298 9 , , , 10_1101-2021_01_07_425737 298 10 Tsuchida Tsuchida NNP 10_1101-2021_01_07_425737 298 11 , , , 10_1101-2021_01_07_425737 298 12 T. T. NNP 10_1101-2021_01_07_425737 298 13 & & CC 10_1101-2021_01_07_425737 298 14 Fukatsu Fukatsu NNP 10_1101-2021_01_07_425737 298 15 , , , 10_1101-2021_01_07_425737 298 16 T. T. NNP 10_1101-2021_01_07_425737 298 17 Cellular cellular JJ 10_1101-2021_01_07_425737 298 18 mechanism mechanism NN 10_1101-2021_01_07_425737 298 19 for for IN 10_1101-2021_01_07_425737 298 20 selective selective JJ 10_1101-2021_01_07_425737 298 21 vertical vertical JJ 10_1101-2021_01_07_425737 298 22 transmission transmission NN 10_1101-2021_01_07_425737 298 23 of of IN 10_1101-2021_01_07_425737 298 24 an an DT 10_1101-2021_01_07_425737 298 25 obligate obligate NN 10_1101-2021_01_07_425737 298 26 insect insect NN 10_1101-2021_01_07_425737 298 27 symbiont symbiont NN 10_1101-2021_01_07_425737 298 28 at at IN 10_1101-2021_01_07_425737 298 29 the the DT 10_1101-2021_01_07_425737 298 30 bacteriocyte bacteriocyte NN 10_1101-2021_01_07_425737 298 31 - - HYPH 10_1101-2021_01_07_425737 298 32 embryo embryo NN 10_1101-2021_01_07_425737 298 33 interface interface NN 10_1101-2021_01_07_425737 298 34 . . . 10_1101-2021_01_07_425737 299 1 Proc Proc NNP 10_1101-2021_01_07_425737 299 2 Natl Natl NNP 10_1101-2021_01_07_425737 299 3 Acad Acad NNP 10_1101-2021_01_07_425737 299 4 Sci Sci NNP 10_1101-2021_01_07_425737 299 5 U U NNP 10_1101-2021_01_07_425737 299 6 S S NNP 10_1101-2021_01_07_425737 299 7 A A NNP 10_1101-2021_01_07_425737 299 8 109 109 CD 10_1101-2021_01_07_425737 299 9 , , , 10_1101-2021_01_07_425737 299 10 E1230 E1230 NNP 10_1101-2021_01_07_425737 299 11 - - HYPH 10_1101-2021_01_07_425737 299 12 7 7 CD 10_1101-2021_01_07_425737 299 13 ( ( -LRB- 10_1101-2021_01_07_425737 299 14 2012 2012 CD 10_1101-2021_01_07_425737 299 15 ) ) -RRB- 10_1101-2021_01_07_425737 299 16 . . . 10_1101-2021_01_07_425737 300 1 ( ( -LRB- 10_1101-2021_01_07_425737 300 2 which which WDT 10_1101-2021_01_07_425737 300 3 was be VBD 10_1101-2021_01_07_425737 300 4 not not RB 10_1101-2021_01_07_425737 300 5 certified certify VBN 10_1101-2021_01_07_425737 300 6 by by IN 10_1101-2021_01_07_425737 300 7 peer peer NN 10_1101-2021_01_07_425737 300 8 review review NN 10_1101-2021_01_07_425737 300 9 ) ) -RRB- 10_1101-2021_01_07_425737 300 10 is be VBZ 10_1101-2021_01_07_425737 300 11 the the DT 10_1101-2021_01_07_425737 300 12 author author NN 10_1101-2021_01_07_425737 300 13 / / SYM 10_1101-2021_01_07_425737 300 14 funder funder NN 10_1101-2021_01_07_425737 300 15 . . . 10_1101-2021_01_07_425737 301 1 All all DT 10_1101-2021_01_07_425737 301 2 rights right NNS 10_1101-2021_01_07_425737 301 3 reserved reserve VBD 10_1101-2021_01_07_425737 301 4 . . . 10_1101-2021_01_07_425737 302 1 No no DT 10_1101-2021_01_07_425737 302 2 reuse reuse NN 10_1101-2021_01_07_425737 302 3 allowed allow VBN 10_1101-2021_01_07_425737 302 4 without without IN 10_1101-2021_01_07_425737 302 5 permission permission NN 10_1101-2021_01_07_425737 302 6 . . . 10_1101-2021_01_07_425737 303 1 The the DT 10_1101-2021_01_07_425737 303 2 copyright copyright NN 10_1101-2021_01_07_425737 303 3 holder holder NN 10_1101-2021_01_07_425737 303 4 for for IN 10_1101-2021_01_07_425737 303 5 this this DT 10_1101-2021_01_07_425737 303 6 preprintthis preprintthis NN 10_1101-2021_01_07_425737 303 7 version version NN 10_1101-2021_01_07_425737 303 8 posted post VBD 10_1101-2021_01_07_425737 303 9 January January NNP 10_1101-2021_01_07_425737 303 10 8 8 CD 10_1101-2021_01_07_425737 303 11 , , , 10_1101-2021_01_07_425737 303 12 2021 2021 CD 10_1101-2021_01_07_425737 303 13 . . . 10_1101-2021_01_07_425737 303 14 ; ; : 10_1101-2021_01_07_425737 303 15 https://doi.org/10.1101/2021.01.07.425737doi https://doi.org/10.1101/2021.01.07.425737doi ADD 10_1101-2021_01_07_425737 303 16 : : : 10_1101-2021_01_07_425737 303 17 bioRxiv biorxiv IN 10_1101-2021_01_07_425737 303 18 preprint preprint NN 10_1101-2021_01_07_425737 303 19 https://doi.org/10.1101/2021.01.07.425737 https://doi.org/10.1101/2021.01.07.425737 XX