id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
10_1101-2021_01_04_425218	Masri, Rana El	Extracellular endosulfatase Sulf-2 harbours a chondroitin/dermatan sulfate chain that modulates its enzyme activity	2021	42	.pdf	application/pdf	12979	1074	58	Extracellular endosulfatase Sulf-2 harbours a chondroitin/dermatan sulfate chain that modulates its enzyme activity In conclusion, our results highlight HSulf-2 as a unique proteoglycan enzyme and its newlyidentified GAG chain as a critical non-catalytic modulator of the enzyme activity. also failed to detect the C-terminal chain containing the enzyme HD domain using PAGE analysis (Figure Nano-scale liquid chromatography MS/MS analysis of trypsinand chondroitinase ABC-digested PGs isolated from the culture medium of human neuroblastoma SHSY5Y cells led to the identification of a HSulf-2 specific, 21 amino acid long glycopeptide highlighting a HSulf-2 GAG chain modulates enzyme activity in vitro These data therefore suggest that HSulf-2 GAG chain may also influence enzyme HSulf-2 GAG chain modulates tumor growth and metastasis in vivo Hsulf-2 GAG chain could also modulate the enzyme function through other mechanisms. We thus next analyzed the effect of HSulf-2 GAG chain in an in vivo mouse xenograft model of cancer 	./cache/10_1101-2021_01_04_425218.pdf	./txt/10_1101-2021_01_04_425218.txt