id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_qkfc6bjbbfaqjalmejcpx2p77u	Peter Brooks	Robust physical methods that enrich genomic regions identical by descent for linkage studies: confirmation of a locus for osteogenesis imperfecta	2009.0	14	.pdf	application/pdf	10375	1004	38	Results: We have improved methods for enriching regions of identity-by-descent (IBD) shared Mapping of regions identical-by-descent (IBD) is a powerful method for the identification of genetic loci shared mapping of IBD regions by hybridization to DNA arrays inherent in a multi-step procedure and the lack of appropriate means to map the IBD-enriched DNA. shared IBD locus that includes COL1A2 bearing the disease-causing mutation and additional loci that may be relevant to OI etiology. After tagging, the DNAs are mixed, denatured and reannealed to form hybrid fragments of different types as shown in Figure 1. The resulting IBD-enriched DNA is generically amplified, labelled and mapped by two-colour hybridization to genomic topographic arrays, using the reannealed Such experiments with pairs of known IBD status validated the enrichment process and the behaviour of immobilized DNA clones. addition, genome-wide mapping of IBD-enriched DNA	./cache/work_qkfc6bjbbfaqjalmejcpx2p77u.pdf	./txt/work_qkfc6bjbbfaqjalmejcpx2p77u.txt