id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_mqertslsgbblvje6u4r4phpzmq	N. Lisitsyn	Cloning the differences between two complex genomes	1993.0	7	.pdf	application/pdf	7550	786	67	A system was developed in which subtractive and kinetic enrichment was used to purify restriction endonuclease fragments present in one population of DNA fragments but not in another. resulted in the isolation of probes to viral genomes present as single copies in human DNA, In RDA, we lowered the DNA complexity of both tester and driver genomes by call "amplicons"; DNA cleaved with relatively infrequent cutting restriction endonucleases was ligated to oligonucleotide Add primer, PCR amplify Agarose gel electrophoresis of difference products of Bgl II amplicons, obtained after Unear amplification 5 ng of purified viral DNA ligated to adaptors (primer set 1; see Table 1). Tester DNA amplicons (lanes a, e, and i) and difference products, after the first (lanes b, Driver and tester amplicons were prepared from human lymphoblastoid cell cultures preparation of tester amplicons and DNA In preparation for the hybridization and amplification step, fragments of tester amplicons were 	./cache/work_mqertslsgbblvje6u4r4phpzmq.pdf	./txt/work_mqertslsgbblvje6u4r4phpzmq.txt