id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_meu327xhajc5lbq6mm4y5gmgae	STEPHEN J. AMISH	RAD sequencing yields a high success rate for westslope cutthroat and rainbow trout species-diagnostic SNP assays	2012.0	8	.pdf	application/pdf	11511	6733	102	cutthroat and rainbow trout species-diagnostic SNP assays the genetic effects of hybridization and introgression requires a large set of high-throughput, diagnostic genetic markers to Recently, we identified several thousand candidate single-nucleotide polymorphism (SNP) markers based on RAD sequencing of 11 westslope cutthroat trout and 13 rainbow trout individuals. we used flanking sequence for 56 of these candidate SNP markers to design high-throughput genotyping assays. Forty-six assays (82%) amplified consistently and allowed easy identification of westslope cutthroat and rainbow trout alleles as well as heterozygote The 46 SNPs will provide high power for early detection of population admixture and improved identification of method for developing and testing SNP markers for nonmodel organisms with limited genomic resources. Keywords: conservation genomics, hybridization, introgression, invasive species, microfluidic PCR, salmonids, SNP, Markers detecting low amounts of admixture in populations and individuals will provide an understanding of RAD sequencing identifies thousands of SNPs for assessing hybridization in rainbow and westslope cutthroat trout.	./cache/work_meu327xhajc5lbq6mm4y5gmgae.pdf	./txt/work_meu327xhajc5lbq6mm4y5gmgae.txt