id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_knbyqj6cxbfl7db22ktmkbbqyu	PAUL A. HOHENLOHE	Next-generation RAD sequencing identifies thousands of SNPs for assessing hybridization between rainbow and westslope cutthroat trout	2011.0	6	.pdf	application/pdf	4312	360	62	The increased numbers of genetic markers produced by genomic techniques have the potential to both identify hybrid We used restriction-site-associated DNA sequencing to identify a dense set of candidate SNP loci with fixed allelic differences between distinguished candidate SNPs from homeologs (paralogs resulting from whole-genome duplication) by detecting excessively high observed heterozygosity and deviations from Hardy–Weinberg proportions. We identified 2923 candidate species-specific SNPs from a single Illumina sequencing lane containing 24 barcode-labelled individuals. data and ongoing genome sequencing of rainbow trout will allow physical mapping of SNP loci for genome-wide scans and will also provide flanking sequence for design of qPCR-based TaqMan� assays for high-throughput, low-cost hybrid This study demonstrates that it is now feasible to identify thousands of informative SNPs in nonmodel species quickly and at reasonable cost, even if no prior genomic information is available. WCT to use in further RAD sequencing studies to examine the genomic patterns of selection and introgression	./cache/work_knbyqj6cxbfl7db22ktmkbbqyu.pdf	./txt/work_knbyqj6cxbfl7db22ktmkbbqyu.txt