id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_2mtnmzyb4nap7bpfbin3ahmori	O. A. Ali	RAD Capture (Rapture): Flexible and Efficient Sequence-Based Genotyping	2015.0	16	.pdf	application/pdf	7553	690	54	RAD Capture (Rapture): Flexible and Efficient Sequence-Based Genotyping improved restriction site-associated DNA (RAD) sequencing protocol and a new method called Rapture (RAD capture). protocol improves versatility by separating RAD tag isolation and sequencing library preparation into two distinct steps. in-solution capture of chosen RAD tags to target sequencing reads to desired loci. sequence capture, i.e., very inexpensive and rapid library preparation for many individuals as well as high specificity in the number and KEYWORDS massively parallel sequencing; restriction-site associated DNA (RAD); sequence capture; genotyping; population genetics; rainbow trout Sequence capture is one method to reduce genome complexity and thereby allow an increased number of individuals Here we develop a much improved RAD sequencing protocol and a new method called Rapture (RAD capture). Our new RAD protocol significantly improved the average number of mapped fragments, the coverage per locus, and the number of loci covered per barcoded	./cache/work_2mtnmzyb4nap7bpfbin3ahmori.pdf	./txt/work_2mtnmzyb4nap7bpfbin3ahmori.txt