New NSF grant to fund research on blinking molecules | News | Notre Dame News | University of Notre Dame Skip To Content Skip To Navigation Skip To Search University of Notre Dame Notre Dame News Experts ND in the News Subscribe About Us Home Contact Search Menu Home › News › New NSF grant to fund research on blinking molecules New NSF grant to fund research on blinking molecules Published: August 06, 2006 Author: William G. Gilroy A team of researchers led by University of Notre Dame physicist Boldizsár Jankó and backed by a new $1.2 million National Science Foundation Nanoscale Interdisciplinary Research Team (NIRT) grant is seeking to solve an enduring mystery in chemical physics. More than a century ago, at the dawn of modern quantum mechanics, the Nobel Prize-winning physicist Niels Bohr predicted so-calledquantum jumps.He predicted that these jumps would be due to electrons making transitions between discrete energy levels of individual atoms and molecules. Although controversial in Bohrs time, such quantum jumps were experimentally observed, and his prediction verified, in the 1980s. More recently, with the development of single molecule imaging techniques in the early 1990s, it has been possible to observe similar jumps in individual molecules. Experimentally, these quantum jumps translate to discrete interruptions of the continuous emission from single molecules, revealing a phenomenon known as florescent intermittency orblinking. However, while certain instances of blinking can be directly ascribed to Bohrs original quantum jumps, many more cases exist where the observed fluorescence intermittency does not follow his predictions. Specifically, in systems as diverse as fluorescent proteins, single-light harvesting complexes, single organic fluorophores, and, most recently, individual inorganic nanostructures, clear deviations from Bohrs predictions occur. As a consequence, virtually all know fluorophores, including fluorescent quantum dots and molecules, exhibit unexplainable episodes of intermittentblinkingin their emission. The underlying quantum mechanical process responsible for this phenomenon is an enduring mystery in modern chemical physics. Now, Jankó and his team of researchers hope to unlock the mysteries of these fluorescent molecules or flourophores. Their results could lead to powerful imaging probes that will enable future researchers to better track disease-related molecules within cells. Fluorescent molecules could be of fundamental importance in imaging biological systems and monitoring dynamic processes in vivo ,Jankó said.One of the most attractive type of flourophores today are semiconductor nanocrystal quantum dots (NQD). Their small size, brightness, photostability and highly tunable fluorescent color make them vastly superior to organic dyes. The blinking phenomenon, however, presents daunting difficulty is using these dots, especially for such applications as single-molecule biological imaging, where a single NQD is used as a fluorescent label. The NQD is fluorescent for some time, a so-called ‘on-time,and then becomes optically inactive, experiencing an ‘off-time,whereupon it turns on again,Jankó said. If the blinking process could be controlled, quantum dots could, for example, provide better, more stable, multi-color imaging of cancer cells or provide researchers with real-time images of a viral infection, such HIV, within a cell. It is very important to elucidate the origin of this phenomenon and to identify ways to control the blinking process,Jankó said. The team already has taken a strong first step toward understanding the phenomenon through research by group member Masaru Kuno, an assistant professor of chemistry and biochemistry at Notre Dame. Kuno has discovered that the on- and off-time intervals of intermittent nanocrystal quantum dots follow a universal power law distribution. This discovery has provided Notre Dame researchers and others with the first hints for developing a deeper insight into the physical mechanism behind the vast range of on- and off-times in the intermittency. James Merz, Frank M. Freimann Professor of Electrical Engineering at Notre Dame, will provide the research team with expertise in low temperature, high magnetic field experiments and innear-fieldscanning optical microscopy of single fluorescent dots. Gregory Snider, a Notre Dame associate professor of engineering, will provide key expertise in single-electron tunneling (SET) device fabrication and operation, necessary to manipulate electrons one-by-one, and to perform ultra-sensitive charge fluctuation measurements on individual fluorophores. And Rusty Landford of the Bioimaging Beckman Institute at the California Institute of Technology will collaborate with the Notre Dame researchers on how to optimize the quantum dots for medical imaging. The flourophore research effort also will feature a strong outreach component under which Notre Dame undergraduates and high school students will have the unique experience of witnessing first-hand single molecule imaging via a low-cost, single molecule-sensitive optical microscope we have developed from off-the-shelf components,Jankó said. The overall goal of the NIRT program is to encourage synergistic science and engineering research in emerging areas of nanoscale science and technology. The award process is extremely competitive with only 4 percent of submitted proposals receiving subsequent NSF funding. Jankó previously received a $1.8-million NIRT grant in 2002 to support research on the development and creation of man-made materials aimed at performing extremely fast functions in future generation computers. * Contact: * _Bolidizsár Jankó, associate professor of physics, 574-631-8049, janko.2@nd.edu _ TopicID: 18522 Home Experts ND in the News Subscribe About Us For the Media Contact Office of Public Affairs and Communications Notre Dame News 500 Grace Hall Notre Dame, IN 46556 USA Facebook Twitter Instagram YouTube Pinterest © 2022 University of Notre Dame Search Mobile App News Events Visit Accessibility Facebook Twitter Instagram YouTube LinkedIn